Rh3DG289800

UBA-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0001428
Physical Location & Seq
Forward (+)
29957177 .. 29961794
4618 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG289800.1

Sequence Viewer

Length: 756 bp
ATGAGTGCTAGGCTTCCTATAATCAAGCTTGTAGACTGTGGAACTGGAATTGAGTGTGATTTATCAATTGAGAACAGGGATGGCATTGTAAAATCCCAGATTGTCTACATAATATCTAGAATTGACGGAAGGTTTCAGAAACTGAGTTTTATGATATCTACAGTACAACTTGCTGGAAGGCGCTATACCACCAACCTTGGGTTCACTAGTGAATCTTCGGCAACTGAACATGGAGGCGATCTCAATGAAGCCGTGAATGCACATTTTAGGGAAGGAGATGTACACAGGAGTTTCAAGCCTTCATTACTACTTGACCGTAGTTATAGTAGAGATCTCTACAATCGGATTGGTTCTGCATTAACTGGTCGGGCACCATCACCTTCACACCCAGGAGAGGTAACTGGGGTTCCTGTAGGTTTCAATGCCAGCAATGAGCAGCCTTATCTCTCAGGACAGATGCCTACAATTCCGGATGTGGCGAGAACTGACTTGGACGCCTCAAGATATGGCAATGATGTGGAGGAAGAAATGATTCAAGCTGCTATTGAAGCTTCAAAACGAGAAGCTGAAATGGGTTCTCCGAATGTGCAAAACAGTACTCCCAATGTATCTCCTGGTAATGGGCTTCCGGTTGAGAAAATTCCTGAAGAAAATTCAGATTTTGATCGTGCAGTTTCGTTGTCCTTGAAGGTTCTTAATCTGTCTTTCTTTTTCTGGGAGGGATACTGGTATTGGGATGAAGGACGAACTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UBA_4 UBX UIM

Protein Analysis

251

Amino Acids

27.75

Weight (kDa)

5.02

Isoelectric Point (pI)

38.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTPAP-like_central PF22600 2 - 39 1.5e-07 Poly(A) RNA polymerase, mitochondrial-like, central palm domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 370
AccI GTMKAC 2 cut(s) 33, 105
AccIII TCCGGA 1 cut(s) 469
AcsI RAATTY 2 cut(s) 639, 652
AcuI CTGAAG 1 cut(s) 666
AcyI GRCGYC 1 cut(s) 495
AfaI GTAC 3 cut(s) 165, 282, 598
AfiI CCNNNNNNNGG 3 cut(s) 198, 394, 620
AgsI TTSAA 6 cut(s) 295, 421, 536, 548, 555, 688
AhlI ACTAGT 1 cut(s) 206
AjnI CCWGG 2 cut(s) 388, 613
AjuI GAANNNNNNNTTGG 2 cut(s) 185, 217
AluBI AGCT 4 cut(s) 28, 539, 551, 566
AluI AGCT 4 cut(s) 28, 539, 551, 566
AlwNI CAGNNNCTG 1 cut(s) 142
Aor13HI TCCGGA 1 cut(s) 469
ApeKI GCWGC 2 cut(s) 436, 539
ApoI RAATTY 2 cut(s) 639, 652
Asp700I GAANNNNTTC 1 cut(s) 531
AspLEI GCGC 1 cut(s) 183
AsuHPI GGTGA 1 cut(s) 369
BaeGI GKGCMC 1 cut(s) 373
BanI GGYRCC 1 cut(s) 370
BarI GAAGNNNNNNTAC 4 cut(s) 169, 201, 264, 296
BbvI GCAGC 2 cut(s) 448, 526
BccI CCATC 2 cut(s) 74, 382
BceAI ACGGC 1 cut(s) 236
BciT130I CCWGG 2 cut(s) 390, 615
BciVI GTATCC 1 cut(s) 716
BcuI ACTAGT 1 cut(s) 206
BfaI CTAG 3 cut(s) 9, 117, 207
BfmI CTRYAG 2 cut(s) 159, 411
BfoI RGCGCY 1 cut(s) 184
BfuI GTATCC 1 cut(s) 716
BglII AGATCT 1 cut(s) 331
BisI GCNGC 2 cut(s) 437, 540
BlsI GCNGC 2 cut(s) 438, 541
BmcAI AGTACT 1 cut(s) 598
Bme1390I CCNGG 2 cut(s) 390, 615
BmiI GGNNCC 2 cut(s) 372, 408
BmrFI CCNGG 2 cut(s) 390, 615
BmrI ACTGGG 1 cut(s) 411
BmsI GCATC 1 cut(s) 447
BmuI ACTGGG 1 cut(s) 411
BplI GAGNNNNNCTC 2 cut(s) 225, 257
BpuEI CTTGAG 1 cut(s) 484
BsaBI GATNNNNATC 1 cut(s) 663
BsaHI GRCGYC 1 cut(s) 495
BsaJI CCNNGG 2 cut(s) 196, 388
BsaWI WCCGGW 2 cut(s) 469, 628
Bsc4I CCNNNNNNNGG 3 cut(s) 198, 394, 620
Bse1I ACTGG 4 cut(s) 49, 367, 406, 731
Bse3DI GCAATG 2 cut(s) 436, 517
Bse8I GATNNNNATC 1 cut(s) 663
BseAI TCCGGA 1 cut(s) 469
BseBI CCWGG 2 cut(s) 390, 615
BseDI CCNNGG 2 cut(s) 196, 388
BseGI GGATG 3 cut(s) 85, 478, 742
BseJI GATNNNNATC 1 cut(s) 663
BseLI CCNNNNNNNGG 3 cut(s) 198, 394, 620
BseMI GCAATG 2 cut(s) 436, 517
BseMII CTCAG 2 cut(s) 134, 462
BseNI ACTGG 4 cut(s) 49, 367, 406, 731
BseSI GKGCMC 1 cut(s) 373
BseXI GCAGC 2 cut(s) 448, 526
BsgI GTGCAG 1 cut(s) 690
BshNI GGYRCC 1 cut(s) 370
BsiSI CCGG 2 cut(s) 470, 629
BslI CCNNNNNNNGG 3 cut(s) 198, 394, 620
BsmI GAATGC 1 cut(s) 262
Bsp1286I GDGCHC 1 cut(s) 373
Bsp13I TCCGGA 1 cut(s) 469
Bsp1407I TGTACA 1 cut(s) 280
Bsp143I GATC 3 cut(s) 238, 331, 664
BspCNI CTCAG 2 cut(s) 135, 461
BspEI TCCGGA 1 cut(s) 469
BspLI GGNNCC 2 cut(s) 372, 408
BspT107I GGYRCC 1 cut(s) 370
BsrDI GCAATG 2 cut(s) 436, 517
BsrGI TGTACA 1 cut(s) 280
BsrI ACTGG 4 cut(s) 49, 367, 406, 731
BssECI CCNNGG 2 cut(s) 196, 388
BssMI GATC 3 cut(s) 238, 331, 664
BssNI GRCGYC 1 cut(s) 495
BssT1I CCWWGG 1 cut(s) 196
Bst2UI CCWGG 2 cut(s) 390, 615
Bst4CI ACNGT 4 cut(s) 38, 163, 317, 596
BstACI GRCGYC 1 cut(s) 495
BstAUI TGTACA 1 cut(s) 280
BstC8I GCNNGC 1 cut(s) 427
BstDEI CTNAG 2 cut(s) 143, 448
BstF5I GGATG 3 cut(s) 85, 478, 742
BstH2I RGCGCY 1 cut(s) 184
BstHHI GCGC 1 cut(s) 183
BstKTI GATC 3 cut(s) 241, 334, 667
BstMBI GATC 3 cut(s) 238, 331, 664
BstMWI GCNNNNNNNGC 2 cut(s) 257, 548
BstNI CCWGG 2 cut(s) 390, 615
BstSCI CCNGG 2 cut(s) 388, 613
BstSFI CTRYAG 2 cut(s) 159, 411
BstSLI GKGCMC 1 cut(s) 373
BstV1I GCAGC 2 cut(s) 448, 526
BstX2I RGATCY 1 cut(s) 331
BstYI RGATCY 1 cut(s) 331
BsuI GTATCC 1 cut(s) 716
BtsCI GGATG 3 cut(s) 85, 478, 742
Cac8I GCNNGC 1 cut(s) 427
CaiI CAGNNNCTG 1 cut(s) 142
CfoI GCGC 1 cut(s) 183
CseI GACGC 1 cut(s) 503
Csp6I GTAC 3 cut(s) 164, 281, 597
CspCI CAANNNNNGTGG 2 cut(s) 178, 213
CviAII CATG 1 cut(s) 230
CviJI RGCY 9 cut(s) 13, 28, 251, 298, 439, 539, 551, 566, 625
CviKI_1 RGCY 9 cut(s) 13, 28, 251, 298, 439, 539, 551, 566, 625
CviQI GTAC 3 cut(s) 164, 281, 597
DdeI CTNAG 2 cut(s) 143, 448
DpnI GATC 3 cut(s) 240, 333, 666
DpnII GATC 3 cut(s) 238, 331, 664
Eco130I CCWWGG 1 cut(s) 196
Eco32I GATATC 1 cut(s) 156
Eco57I CTGAAG 1 cut(s) 666
EcoRII CCWGG 2 cut(s) 388, 613
EcoRV GATATC 1 cut(s) 156
EcoT14I CCWWGG 1 cut(s) 196
ErhI CCWWGG 1 cut(s) 196
FaeI CATG 1 cut(s) 233
FaiI YATR 7 cut(s) 20, 110, 152, 186, 231, 324, 507
FatI CATG 1 cut(s) 229
FblI GTMKAC 2 cut(s) 33, 105
Fnu4HI GCNGC 2 cut(s) 437, 540
FokI GGATG 3 cut(s) 92, 485, 749
Fsp4HI GCNGC 2 cut(s) 437, 540
FspBI CTAG 3 cut(s) 9, 117, 207
GlaI GCGC 1 cut(s) 182
GluI GCNGC 2 cut(s) 437, 540
HaeII RGCGCY 1 cut(s) 184
HapII CCGG 2 cut(s) 470, 629
HgaI GACGC 1 cut(s) 503
HhaI GCGC 1 cut(s) 183
Hin1I GRCGYC 1 cut(s) 495
Hin1II CATG 1 cut(s) 233
Hin6I GCGC 1 cut(s) 181
HinP1I GCGC 1 cut(s) 181
HindIII AAGCTT 2 cut(s) 26, 549
HinfI GANTC 2 cut(s) 212, 532
HpaII CCGG 2 cut(s) 470, 629
HphI GGTGA 1 cut(s) 369
Hpy166II GTNNAC 4 cut(s) 34, 106, 204, 283
Hpy188I TCNGA 4 cut(s) 138, 345, 582, 658
Hpy188III TCNNGA 5 cut(s) 117, 450, 470, 501, 644
Hpy8I GTNNAC 4 cut(s) 34, 106, 204, 283
HpyAV CCTTC 7 cut(s) 123, 171, 266, 309, 390, 682, 734
HpyCH4III ACNGT 4 cut(s) 38, 163, 317, 596
HpyCH4V TGCA 4 cut(s) 260, 356, 589, 671
HpyF10VI GCNNNNNNNGC 2 cut(s) 257, 548
HpyF3I CTNAG 2 cut(s) 143, 448
Hsp92I GRCGYC 1 cut(s) 495
Hsp92II CATG 1 cut(s) 233
HspAI GCGC 1 cut(s) 181
Kpn2I TCCGGA 1 cut(s) 469
Kzo9I GATC 3 cut(s) 238, 331, 664
Lsp1109I GCAGC 2 cut(s) 448, 526
LweI GCATC 1 cut(s) 447
MaeI CTAG 3 cut(s) 9, 117, 207
MaeIII GTNAC 1 cut(s) 397
MalI GATC 3 cut(s) 240, 333, 666
MboI GATC 3 cut(s) 238, 331, 664
MboII GAAGA 3 cut(s) 207, 536, 659
MfeI CAATTG 1 cut(s) 66
MflI RGATCY 1 cut(s) 331
MhlI GDGCHC 1 cut(s) 373
MluCI AATT 6 cut(s) 48, 66, 120, 465, 639, 652
MnlI CCTC 5 cut(s) 227, 388, 508, 514, 712
MroI TCCGGA 1 cut(s) 469
MroXI GAANNNNTTC 1 cut(s) 531
MseI TTAA 2 cut(s) 359, 696
MspI CCGG 2 cut(s) 470, 629
MspR9I CCNGG 2 cut(s) 390, 615
MunI CAATTG 1 cut(s) 66
Mva1269I GAATGC 1 cut(s) 262
MvaI CCWGG 2 cut(s) 390, 615
MwoI GCNNNNNNNGC 2 cut(s) 257, 548
NdeII GATC 3 cut(s) 238, 331, 664
NlaIII CATG 1 cut(s) 233
NlaIV GGNNCC 2 cut(s) 372, 408
PctI GAATGC 1 cut(s) 262
PdmI GAANNNNTTC 1 cut(s) 531
PfeI GAWTC 2 cut(s) 212, 532
PkrI GCNGC 2 cut(s) 438, 541
Psp6I CCWGG 2 cut(s) 388, 613
PspGI CCWGG 2 cut(s) 388, 613
PspN4I GGNNCC 2 cut(s) 372, 408
PsrI GAACNNNNNNTAC 2 cut(s) 390, 422
PstNI CAGNNNCTG 1 cut(s) 142
PsuI RGATCY 1 cut(s) 331
RsaI GTAC 3 cut(s) 165, 282, 598
RsaNI GTAC 3 cut(s) 164, 281, 597
SaqAI TTAA 2 cut(s) 359, 696
SatI GCNGC 2 cut(s) 437, 540
Sau3AI GATC 3 cut(s) 238, 331, 664
ScaI AGTACT 1 cut(s) 598
ScrFI CCNGG 2 cut(s) 390, 615
SduI GDGCHC 1 cut(s) 373
SfaNI GCATC 1 cut(s) 447
SfcI CTRYAG 2 cut(s) 159, 411
SmlI CTYRAG 1 cut(s) 499
SmoI CTYRAG 1 cut(s) 499
SpeI ACTAGT 1 cut(s) 206
Sse9I AATT 6 cut(s) 48, 66, 120, 465, 639, 652
SspMI CTAG 3 cut(s) 9, 117, 207
StyD4I CCNGG 2 cut(s) 388, 613
StyI CCWWGG 1 cut(s) 196
TaaI ACNGT 4 cut(s) 38, 163, 317, 596
TasI AATT 6 cut(s) 48, 66, 120, 465, 639, 652
TatI WGTACW 3 cut(s) 163, 280, 596
TfiI GAWTC 2 cut(s) 212, 532
Tru1I TTAA 2 cut(s) 359, 696
Tru9I TTAA 2 cut(s) 359, 696
TseI GCWGC 2 cut(s) 436, 539
TspDTI ATGAA 3 cut(s) 261, 291, 753
TspGWI ACGGA 1 cut(s) 141
XapI RAATTY 2 cut(s) 639, 652
XbaI TCTAGA 1 cut(s) 116
XmiI GTMKAC 2 cut(s) 33, 105
XmnI GAANNNNTTC 1 cut(s) 531
XspI CTAG 3 cut(s) 9, 117, 207
ZrmI AGTACT 1 cut(s) 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.