Rorug04G0062900

UBA-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001428
Physical Location & Seq
Forward (+)
9840455 .. 9840961
507 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0062900.1

Sequence Viewer

Length: 507 bp
ATGGAATACCTCACTAACCTTCGTCAGCTGGGGATTAGACACTCTGGAAACCTGGAGTCCTTGCCACCTAACATGAAGAACTTGACTGCATTACATACTTTGGTGATCGATGATTGTAAGAAGCTTGAGTTAATAAGGTCGGGAGAAGGCATTAAAGGTCTTCAATCATTTCTTATCCACGGTTGTTCAAATTTGGAGGCTTATTTGCCTAATTGGCTTCAAGAGTCTGTAGACACTTTACAGAGCATGCTAGTCACGCAATGTGATAATCTCACGGCACTTCCATATTGGTTGCAAAATCTCACCTTGCTGGAGCAACTGATGATTGGGGACTGCCCCAAATTGTCTGCTTTGCCGGAAGGGATGCATCACCTCACTGCGTTAAGAGACTTGCGGATTTGCGAGTGTCCTGAGTTGAGCAAAAGATGCAAGAGGGAAATAGGAGAGGATTGGTCCAAGATCAAACATGTCCCAAAAATTGTACTCGACGACTCTGACGATGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UBA_4 UBX UIM

Protein Analysis

168

Amino Acids

19.02

Weight (kDa)

5.47

Isoelectric Point (pI)

50.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 3 - 156 1.4e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 231
AciI CCGC 1 cut(s) 394
AcsI RAATTY 1 cut(s) 190
AfaI GTAC 1 cut(s) 483
AflIII ACRYGT 1 cut(s) 466
AgsI TTSAA 3 cut(s) 164, 189, 221
AjnI CCWGG 1 cut(s) 51
AluBI AGCT 2 cut(s) 28, 124
AluI AGCT 2 cut(s) 28, 124
Alw26I GTCTC 1 cut(s) 381
ApoI RAATTY 1 cut(s) 190
AspS9I GGNCC 1 cut(s) 453
AsuHPI GGTGA 3 cut(s) 115, 295, 362
AvaII GGWCC 1 cut(s) 453
BbsI GAAGAC 1 cut(s) 152
BccI CCATC 1 cut(s) 494
BceAI ACGGC 1 cut(s) 291
BciT130I CCWGG 1 cut(s) 53
BcoDI GTCTC 1 cut(s) 381
BfaI CTAG 1 cut(s) 251
BfmI CTRYAG 1 cut(s) 228
BglI GCCNNNNNGGC 1 cut(s) 214
Bme1390I CCNGG 1 cut(s) 53
Bme18I GGWCC 1 cut(s) 453
BmgT120I GGNCC 1 cut(s) 453
BmrFI CCNGG 1 cut(s) 53
BmsI GCATC 3 cut(s) 354, 376, 416
BpiI GAAGAC 1 cut(s) 152
BpmI CTGGAG 2 cut(s) 74, 332
BpuEI CTTGAG 1 cut(s) 146
Bsa29I ATCGAT 1 cut(s) 108
BsaJI CCNNGG 1 cut(s) 178
Bse3DI GCAATG 1 cut(s) 266
BseBI CCWGG 1 cut(s) 53
BseCI ATCGAT 1 cut(s) 108
BseDI CCNNGG 1 cut(s) 178
BseGI GGATG 1 cut(s) 369
BseMI GCAATG 1 cut(s) 266
BseMII CTCAG 1 cut(s) 402
BseYI CCCAGC 1 cut(s) 28
BshVI ATCGAT 1 cut(s) 108
BsiSI CCGG 1 cut(s) 356
BslFI GGGAC 2 cut(s) 344, 455
BsmAI GTCTC 1 cut(s) 381
BsmFI GGGAC 2 cut(s) 344, 455
Bsp143I GATC 2 cut(s) 105, 459
BspACI CCGC 1 cut(s) 394
BspCNI CTCAG 1 cut(s) 403
BspDI ATCGAT 1 cut(s) 108
BsrDI GCAATG 1 cut(s) 266
BssECI CCNNGG 1 cut(s) 178
BssMI GATC 2 cut(s) 105, 459
Bst2UI CCWGG 1 cut(s) 53
Bst4CI ACNGT 1 cut(s) 182
BstAPI GCANNNNNTGC 1 cut(s) 426
BstC8I GCNNGC 1 cut(s) 248
BstDEI CTNAG 1 cut(s) 411
BstDSI CCRYGG 1 cut(s) 178
BstF5I GGATG 1 cut(s) 369
BstKTI GATC 2 cut(s) 108, 462
BstMAI GTCTC 1 cut(s) 381
BstMBI GATC 2 cut(s) 105, 459
BstMWI GCNNNNNNNGC 3 cut(s) 214, 256, 426
BstNI CCWGG 1 cut(s) 53
BstNSI RCATGY 2 cut(s) 250, 470
BstSCI CCNGG 1 cut(s) 51
BstSFI CTRYAG 1 cut(s) 228
BstV2I GAAGAC 1 cut(s) 152
Bsu15I ATCGAT 1 cut(s) 108
BsuTUI ATCGAT 1 cut(s) 108
BtgI CCRYGG 1 cut(s) 178
BtsCI GGATG 1 cut(s) 369
BtsI GCAGTG 1 cut(s) 375
BtsIMutI CAGTG 1 cut(s) 375
Cac8I GCNNGC 1 cut(s) 248
Cfr13I GGNCC 1 cut(s) 453
ClaI ATCGAT 1 cut(s) 108
Csp6I GTAC 1 cut(s) 482
CviAII CATG 3 cut(s) 73, 247, 467
CviJI RGCY 4 cut(s) 28, 124, 200, 217
CviKI_1 RGCY 4 cut(s) 28, 124, 200, 217
CviQI GTAC 1 cut(s) 482
DdeI CTNAG 1 cut(s) 411
DpnI GATC 2 cut(s) 107, 461
DpnII GATC 2 cut(s) 105, 459
Eco47I GGWCC 1 cut(s) 453
EcoRII CCWGG 1 cut(s) 51
EcoT22I ATGCAT 1 cut(s) 369
FaeI CATG 3 cut(s) 76, 250, 470
FaiI YATR 5 cut(s) 74, 96, 248, 286, 468
FaqI GGGAC 2 cut(s) 344, 455
FatI CATG 3 cut(s) 72, 246, 466
FblI GTMKAC 1 cut(s) 231
FokI GGATG 1 cut(s) 376
FspBI CTAG 1 cut(s) 251
GsaI CCCAGC 1 cut(s) 32
GsuI CTGGAG 2 cut(s) 74, 332
HapII CCGG 1 cut(s) 356
Hin1II CATG 3 cut(s) 76, 250, 470
HindIII AAGCTT 1 cut(s) 122
HinfI GANTC 3 cut(s) 56, 224, 491
HpaII CCGG 1 cut(s) 356
HphI GGTGA 3 cut(s) 115, 295, 362
Hpy166II GTNNAC 1 cut(s) 232
Hpy188I TCNGA 1 cut(s) 496
Hpy188III TCNNGA 4 cut(s) 45, 141, 221, 410
Hpy8I GTNNAC 1 cut(s) 232
Hpy99I CGWCG 1 cut(s) 491
HpyAV CCTTC 3 cut(s) 29, 140, 353
HpyCH4III ACNGT 1 cut(s) 182
HpyCH4V TGCA 4 cut(s) 89, 295, 367, 429
HpyF10VI GCNNNNNNNGC 3 cut(s) 214, 256, 426
HpyF3I CTNAG 1 cut(s) 411
Hsp92II CATG 3 cut(s) 76, 250, 470
Kzo9I GATC 2 cut(s) 105, 459
LmnI GCTCC 1 cut(s) 313
LpnPI CCDG 7 cut(s) 14, 30, 38, 65, 296, 369, 423
LweI GCATC 3 cut(s) 354, 376, 416
MaeI CTAG 1 cut(s) 251
MaeIII GTNAC 1 cut(s) 253
MalI GATC 2 cut(s) 107, 461
MboI GATC 2 cut(s) 105, 459
MboII GAAGA 2 cut(s) 88, 152
MluCI AATT 4 cut(s) 190, 211, 341, 477
MlyI GAGTC 3 cut(s) 65, 233, 485
MnlI CCTC 5 cut(s) 20, 190, 383, 426, 439
Mph1103I ATGCAT 1 cut(s) 369
MseI TTAA 3 cut(s) 131, 153, 383
MspA1I CMGCKG 1 cut(s) 28
MspI CCGG 1 cut(s) 356
MspR9I CCNGG 1 cut(s) 53
MvaI CCWGG 1 cut(s) 53
MwoI GCNNNNNNNGC 3 cut(s) 214, 256, 426
NdeII GATC 2 cut(s) 105, 459
NlaIII CATG 3 cut(s) 76, 250, 470
NmuCI GTSAC 1 cut(s) 253
NsiI ATGCAT 1 cut(s) 369
NspI RCATGY 2 cut(s) 250, 470
PaeI GCATGC 1 cut(s) 250
PciI ACATGT 1 cut(s) 466
PcsI WCGNNNNNNNCGW 1 cut(s) 495
PleI GAGTC 3 cut(s) 64, 232, 485
PpsI GAGTC 3 cut(s) 64, 232, 485
PscI ACATGT 1 cut(s) 466
Psp6I CCWGG 1 cut(s) 51
PspFI CCCAGC 1 cut(s) 28
PspGI CCWGG 1 cut(s) 51
PspPI GGNCC 1 cut(s) 453
PvuII CAGCTG 1 cut(s) 28
RsaI GTAC 1 cut(s) 483
RsaNI GTAC 1 cut(s) 482
SaqAI TTAA 3 cut(s) 131, 153, 383
Sau3AI GATC 2 cut(s) 105, 459
Sau96I GGNCC 1 cut(s) 453
SchI GAGTC 3 cut(s) 65, 233, 485
ScrFI CCNGG 1 cut(s) 53
SfaNI GCATC 3 cut(s) 354, 376, 416
SfcI CTRYAG 1 cut(s) 228
SinI GGWCC 1 cut(s) 453
SmlI CTYRAG 1 cut(s) 125
SmoI CTYRAG 1 cut(s) 125
SphI GCATGC 1 cut(s) 250
Sse9I AATT 4 cut(s) 190, 211, 341, 477
SsiI CCGC 1 cut(s) 394
SspMI CTAG 1 cut(s) 251
StyD4I CCNGG 1 cut(s) 51
TaaI ACNGT 1 cut(s) 182
TaqI TCGA 2 cut(s) 108, 486
TasI AATT 4 cut(s) 190, 211, 341, 477
TatI WGTACW 1 cut(s) 481
Tru1I TTAA 3 cut(s) 131, 153, 383
Tru9I TTAA 3 cut(s) 131, 153, 383
TscAI CASTG 1 cut(s) 382
TseFI GTSAC 1 cut(s) 253
Tsp45I GTSAC 1 cut(s) 253
TspDTI ATGAA 1 cut(s) 89
TspRI CASTG 1 cut(s) 382
VpaK11BI GGWCC 1 cut(s) 453
XapI RAATTY 1 cut(s) 190
XceI RCATGY 2 cut(s) 250, 470
XmiI GTMKAC 1 cut(s) 231
XspI CTAG 1 cut(s) 251
Zsp2I ATGCAT 1 cut(s) 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.