RchiOBHm_Chr1g0329331

UBA-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001428
Physical Location & Seq
Reverse (-)
18821235 .. 18824506
3272 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55866

Sequence Viewer

Length: 516 bp
ATGAGTAATCAGAATCAAGTAGCTCCACAACAAGGACTTATGCCACTTCTCTCTGTTGCCAGGAGTTTCAAGCCTTCATTACTACTTGACCGTAGTTATAGTAGAGATCTCTACAATCGGATTGGTTCTGCATTAACTGGTCGGGCACCATCACCTTCACACCCAGGAGAGGTAACTGGGGTTCCTGTAGGTTTCAATGCCAGCAATGAGCAGCCTTATCTCTCAGGACAGATGCCTACAATTCCGGATGTGGCAAGAACTGACTTGGACGCCTCAAGATATGGCAATGATGTGGAGGAAGAAATGATTCAAGCTGCTATTGAAGCTTCAAAACGAGAAGCTGAAATGGGTTCTCCGAATGTGCAAAACAGTACTCCCAATGTATCTCCTGGTAATGGGCTTCCGGTTGAGAAAATTCCTGAAGAAAATTCAGATTTTGATCGTGCAGTTTCGTTGTCCTTGAAGGTTCTTAATCTGTCTTTCTTTTTCTGGGAGGTGGCTGCTTCCTTATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UBA_4 UBX UIM

Protein Analysis

171

Amino Acids

18.54

Weight (kDa)

4.68

Isoelectric Point (pI)

47.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 145
AccIII TCCGGA 1 cut(s) 244
AcsI RAATTY 2 cut(s) 414, 427
AcuI CTGAAG 1 cut(s) 441
AcyI GRCGYC 1 cut(s) 270
AfaI GTAC 1 cut(s) 373
AfiI CCNNNNNNNGG 3 cut(s) 32, 169, 395
AgsI TTSAA 6 cut(s) 70, 196, 311, 323, 330, 463
AjnI CCWGG 3 cut(s) 59, 163, 388
AluBI AGCT 4 cut(s) 23, 314, 326, 341
AluI AGCT 4 cut(s) 23, 314, 326, 341
Aor13HI TCCGGA 1 cut(s) 244
ApeKI GCWGC 3 cut(s) 211, 314, 500
ApoI RAATTY 2 cut(s) 414, 427
Asp700I GAANNNNTTC 1 cut(s) 306
AsuHPI GGTGA 1 cut(s) 144
BaeGI GKGCMC 1 cut(s) 148
BanI GGYRCC 1 cut(s) 145
BbvI GCAGC 3 cut(s) 223, 301, 487
BccI CCATC 1 cut(s) 157
BciT130I CCWGG 3 cut(s) 61, 165, 390
BfmI CTRYAG 1 cut(s) 186
BglII AGATCT 1 cut(s) 106
BisI GCNGC 3 cut(s) 212, 315, 501
BlsI GCNGC 3 cut(s) 213, 316, 502
BmcAI AGTACT 1 cut(s) 373
Bme1390I CCNGG 3 cut(s) 61, 165, 390
BmiI GGNNCC 2 cut(s) 147, 183
BmrFI CCNGG 3 cut(s) 61, 165, 390
BmrI ACTGGG 1 cut(s) 186
BmsI GCATC 1 cut(s) 222
BmuI ACTGGG 1 cut(s) 186
BpuEI CTTGAG 1 cut(s) 259
BsaBI GATNNNNATC 1 cut(s) 438
BsaHI GRCGYC 1 cut(s) 270
BsaJI CCNNGG 1 cut(s) 163
BsaWI WCCGGW 2 cut(s) 244, 403
Bsc4I CCNNNNNNNGG 3 cut(s) 32, 169, 395
Bse1I ACTGG 2 cut(s) 142, 181
Bse3DI GCAATG 2 cut(s) 211, 292
Bse8I GATNNNNATC 1 cut(s) 438
BseAI TCCGGA 1 cut(s) 244
BseBI CCWGG 3 cut(s) 61, 165, 390
BseDI CCNNGG 1 cut(s) 163
BseGI GGATG 1 cut(s) 253
BseJI GATNNNNATC 1 cut(s) 438
BseLI CCNNNNNNNGG 3 cut(s) 32, 169, 395
BseMI GCAATG 2 cut(s) 211, 292
BseMII CTCAG 1 cut(s) 237
BseNI ACTGG 2 cut(s) 142, 181
BseSI GKGCMC 1 cut(s) 148
BseXI GCAGC 3 cut(s) 223, 301, 487
BsgI GTGCAG 1 cut(s) 465
BshNI GGYRCC 1 cut(s) 145
BsiSI CCGG 2 cut(s) 245, 404
BslI CCNNNNNNNGG 3 cut(s) 32, 169, 395
Bsp1286I GDGCHC 1 cut(s) 148
Bsp13I TCCGGA 1 cut(s) 244
Bsp143I GATC 2 cut(s) 106, 439
BspCNI CTCAG 1 cut(s) 236
BspEI TCCGGA 1 cut(s) 244
BspLI GGNNCC 2 cut(s) 147, 183
BspT107I GGYRCC 1 cut(s) 145
BsrDI GCAATG 2 cut(s) 211, 292
BsrI ACTGG 2 cut(s) 142, 181
BssECI CCNNGG 1 cut(s) 163
BssMI GATC 2 cut(s) 106, 439
BssNI GRCGYC 1 cut(s) 270
Bst2UI CCWGG 3 cut(s) 61, 165, 390
Bst4CI ACNGT 2 cut(s) 92, 371
BstACI GRCGYC 1 cut(s) 270
BstC8I GCNNGC 1 cut(s) 202
BstDEI CTNAG 1 cut(s) 223
BstF5I GGATG 1 cut(s) 253
BstKTI GATC 2 cut(s) 109, 442
BstMBI GATC 2 cut(s) 106, 439
BstMWI GCNNNNNNNGC 1 cut(s) 323
BstNI CCWGG 3 cut(s) 61, 165, 390
BstSCI CCNGG 3 cut(s) 59, 163, 388
BstSFI CTRYAG 1 cut(s) 186
BstSLI GKGCMC 1 cut(s) 148
BstV1I GCAGC 3 cut(s) 223, 301, 487
BstX2I RGATCY 1 cut(s) 106
BstYI RGATCY 1 cut(s) 106
BtsCI GGATG 1 cut(s) 253
Cac8I GCNNGC 1 cut(s) 202
CseI GACGC 1 cut(s) 278
Csp6I GTAC 1 cut(s) 372
CviJI RGCY 8 cut(s) 23, 73, 214, 314, 326, 341, 400, 500
CviKI_1 RGCY 8 cut(s) 23, 73, 214, 314, 326, 341, 400, 500
CviQI GTAC 1 cut(s) 372
DdeI CTNAG 1 cut(s) 223
DpnI GATC 2 cut(s) 108, 441
DpnII GATC 2 cut(s) 106, 439
Eco57I CTGAAG 1 cut(s) 441
EcoRII CCWGG 3 cut(s) 59, 163, 388
FaiI YATR 3 cut(s) 41, 99, 282
Fnu4HI GCNGC 3 cut(s) 212, 315, 501
FokI GGATG 1 cut(s) 260
Fsp4HI GCNGC 3 cut(s) 212, 315, 501
GluI GCNGC 3 cut(s) 212, 315, 501
HapII CCGG 2 cut(s) 245, 404
HgaI GACGC 1 cut(s) 278
Hin1I GRCGYC 1 cut(s) 270
HindIII AAGCTT 1 cut(s) 324
HinfI GANTC 2 cut(s) 13, 307
HpaII CCGG 2 cut(s) 245, 404
HphI GGTGA 1 cut(s) 144
Hpy188I TCNGA 4 cut(s) 12, 120, 357, 433
Hpy188III TCNNGA 4 cut(s) 225, 245, 276, 419
HpyAV CCTTC 3 cut(s) 84, 165, 457
HpyCH4III ACNGT 2 cut(s) 92, 371
HpyCH4V TGCA 3 cut(s) 131, 364, 446
HpyF10VI GCNNNNNNNGC 1 cut(s) 323
HpyF3I CTNAG 1 cut(s) 223
Hsp92I GRCGYC 1 cut(s) 270
Kpn2I TCCGGA 1 cut(s) 244
Kzo9I GATC 2 cut(s) 106, 439
LmnI GCTCC 1 cut(s) 28
Lsp1109I GCAGC 3 cut(s) 223, 301, 487
LweI GCATC 1 cut(s) 222
MaeIII GTNAC 1 cut(s) 172
MalI GATC 2 cut(s) 108, 441
MboI GATC 2 cut(s) 106, 439
MboII GAAGA 2 cut(s) 311, 434
MflI RGATCY 1 cut(s) 106
MhlI GDGCHC 1 cut(s) 148
MluCI AATT 3 cut(s) 240, 414, 427
MnlI CCTC 4 cut(s) 163, 283, 289, 487
MroI TCCGGA 1 cut(s) 244
MroXI GAANNNNTTC 1 cut(s) 306
MseI TTAA 2 cut(s) 134, 471
MspI CCGG 2 cut(s) 245, 404
MspR9I CCNGG 3 cut(s) 61, 165, 390
MvaI CCWGG 3 cut(s) 61, 165, 390
MwoI GCNNNNNNNGC 1 cut(s) 323
NdeII GATC 2 cut(s) 106, 439
NlaIV GGNNCC 2 cut(s) 147, 183
PdmI GAANNNNTTC 1 cut(s) 306
PfeI GAWTC 2 cut(s) 13, 307
PkrI GCNGC 3 cut(s) 213, 316, 502
Psp6I CCWGG 3 cut(s) 59, 163, 388
PspGI CCWGG 3 cut(s) 59, 163, 388
PspN4I GGNNCC 2 cut(s) 147, 183
PsrI GAACNNNNNNTAC 2 cut(s) 165, 197
PsuI RGATCY 1 cut(s) 106
RsaI GTAC 1 cut(s) 373
RsaNI GTAC 1 cut(s) 372
SaqAI TTAA 2 cut(s) 134, 471
SatI GCNGC 3 cut(s) 212, 315, 501
Sau3AI GATC 2 cut(s) 106, 439
ScaI AGTACT 1 cut(s) 373
ScrFI CCNGG 3 cut(s) 61, 165, 390
SduI GDGCHC 1 cut(s) 148
SetI ASST 9 cut(s) 25, 157, 174, 193, 316, 328, 343, 468, 498
SfaNI GCATC 1 cut(s) 222
SfcI CTRYAG 1 cut(s) 186
SmlI CTYRAG 1 cut(s) 274
SmoI CTYRAG 1 cut(s) 274
Sse9I AATT 3 cut(s) 240, 414, 427
StyD4I CCNGG 3 cut(s) 59, 163, 388
TaaI ACNGT 2 cut(s) 92, 371
TasI AATT 3 cut(s) 240, 414, 427
TatI WGTACW 1 cut(s) 371
TfiI GAWTC 2 cut(s) 13, 307
Tru1I TTAA 2 cut(s) 134, 471
Tru9I TTAA 2 cut(s) 134, 471
TseI GCWGC 3 cut(s) 211, 314, 500
TspDTI ATGAA 1 cut(s) 66
XapI RAATTY 2 cut(s) 414, 427
XmnI GAANNNNTTC 1 cut(s) 306
ZrmI AGTACT 1 cut(s) 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.