Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr2

Orthogroup ID

OG0001615

Physical Location & Seq

Genomic Coordinates Forward (+)

5981852 .. 5987092

Gene Structure 5241 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3663 bp

ATGGTTTTTTTTTTTTTTTTTGAAAGGGGTGTGATATCCACACACCCCATTTTACTTTTCATACACCTTTTTAATTTTCAGCCTATCAAGGAGTGTGTGAGAAGTAAAATGGGGTGTGTGGATAGCACACCCCTTTTGAAAATTATAAAAACCCTAAATCCATTACTAATTTTTATTTTAAAAAATTATTTTGATCAAAATCTAAAAACCAATAAGGGATTAGATACTAATTTTTCCTCTAAGTTATTAATTTATTTGCTGATATTTTTGAAATTTTCAGTGCATTTGGAGCAAAATAGGAAGATCACTTTCCAGGTAAAAGTTGTACCCTTTTTAGAGACGGTTGTTCACAAAGCGGAACTGACAAGTACACAAGTAACTTGTTTTGGTTTGAATTGGATTTTCAGGTACATATGTAAGCATGCCTGTGCTTATTTTCCCGTGACCGCTCTTATGGTATCTTCTATTTTCATCGTTGATACTAGCATATGTATATGTGCACACACAAAGTGTGTGAGAAAATTTTTTAATTTTGTTTTTGAAATAGAAGGAGAGAGGAAGAGACTGATAGAGATTATATATGTTATGATAGGTTTTGAAAAAAAAAAACAACAAAATTTGATTATTAATTTCATAAAGTTTTGGTTGACAATGAATATTATATTAATTAGTAGGGATATAAAAGTAAAACTGGGATATAAACCACGGTTTAGTGTCCTTCCAGTTCGATTTTTGAGTAAAAGATTTTTAAAATATATTTTCTTTCAGGTCGCAGGTAAGGTCATAATTACTCGTTATTTTAACGAGTATTACACCACACGACCCGTTAATATATCGGATATGACACGAAAACAATATAAACACGGAAAACACGACACGACACGACTGCTAGGTCTAATTGTGACTCGTCAAATTTGTAATATGAGTGGCATTTGGCTCTCTGTGGTTCATGCAGTCTTTGGTTATGAACCACATTCGGTTTTAGCAATTGGTGTTGTCGCACTTGCTGACCTTACCGGGTTCTTGCCTCTCACCAAAATCAAGACCCTTGCGAGTAGCGCGGTAAAATTTCTTTGCTTCGTTGGCACAATCCTTTCTTCTTTTTATATCATGTCTCGACCTTCTTTCTTACAAATTTTTAGCTATAATTTACATATATGGACATGGTTGGGTTTTTCACCGGCCACAAGGAGTAATTTTATCTCCCACTTATCCGCCGGTTATAGTTGCATCATTGTGCCTGGTGGAGTGCGGGAGACATTTTACATGGAGCCTGGCACTGAGGTTTACATCTACAAAATCTTTCATGTGCTCTTGCTGTACCTTTTCATCAAGTCATCTGTTGCTATATGCACTAATCTGACAATCAGCTGGTGTCTGTTTTCTCAATTGATTTGTTTGCATAGCCATGGAGATGGGCCATCCCCTGGTTCCAGTTTTCTGCTTTGGTCAGGTATCACCATTTTCTATCTAGTTTTTGCACCCATTAAGTCATCTACAAAGTTAGCCAGTTGCATAATATCTGTTTTGAATCTGTTAATTACTGGCTTTTCTGGTCCAAACCTTTTTGTGTGGTTTCACGCATCTAATTTCGGGATTATTAAAAGGGCCGTTACGTTTATTATATCTTTAGTTGTTGATTGTACTTTTCTTTTTTCTCTCATGCATGTATATCATCTAGCAATGTTGCAGTCAAATGTATACAAATGGTGGAGGCCAAGTCGGGATTTAGTTTGGAAGATTGCTAGAGCTCTCAAGTTCCCCCCAGTTTTCTTCTGGGGAATATTTGGGTATCTTTGGAAAGTATGTCTGCAGAAACTGAAAGGAAAAACTATTCCGCTGAAAAACGTTCCCGTTAGATTGAGAAGAAATTATACTACTATTAAAGCATTATTTACTTTTACTAACCAAGCAAGGAATTTTTTTTTTAATTCAAATTTTGCGTTCGACCTACTCTCAGCACTACGGTTTCAACTGTTAATGGTTTATATCATGCTTCTCCGCTGCAGAACTCCTGTACCCTACAGTCATCCAATGCACGTGGTGGTCGGAAAACCAATCGATCTCAAGAAAACTCCGCAACCCACCATGGAAGAGTCCCCTGTGAACATTCAATCATGGAGAACCGGTGCTTGGACCGTATTCATATTACTTTATTGCACTCTTTCTGCTGCCCTGGACTTCATCTTCTATGCCTTTATCCCACTAAGTGAAGTCGGTTATATGAATCCTAGAACGTCACCGCACTTGGTTTTGCACCATGTCTTCTGTAAGCTCCGAGTCCTCCTCCACCATTTTTGCTCTGGCCTCCGTCTCATAGTCGCATCTTCTAACAGGAGCATCTGTAGGTTTTCCGTTTCTGTTACAAAAAACTACCTCAACTATTTGGGTTACGACAGTTTCATACCTCATCTTTTAAAATTAACAATGTCATACCGCATCCTACGAATTTGTGACAATGTCATACCTCTGTCAATTTTTCTGTTAAGTGCTGACGTGGCAAGAGACAGGGACCACTCACTAATCCAAACAAAAAAATTTCCTAGAGGTGCGGCATCCCCTTTCCCCTCTCCTTCTTCTTCTCCTTCTTCTTCTTCTCCTCCTTCTTCCTTCTTCTGGTTTTTTTTTTTTTTTTTCTGGGTTGCAGTTTCCATATTTTTTTCCTCCTCCTCTTCTTCCTCCTTCTTCTTCTTCTTCGTTTTTAATACAAAGTGGTCCCAGACTCTTGCCACGTCAGCACTTAACAGAAAAACTAACAGAAAAATTGACGGAGGTATGACATTGTCACAAATTCGTAAGATGTGGTATGACATTTTTTTGTACTTTACCCTATCTTTAATTGCGACCACTTCTACGCTACCTCGGATATCCTCGTTGGTTTTGTTTGTGCAATTGAACCTCCATTCGATTCACATCTGGATTCACATCTCTTATCTTCTACAGAGCAGTAGCATGTGGTTTTACATATTTATAAAGCTTTTTCATAGCGAAATACATGCAAGTGAAGTTTTAAATGTACCGGAAATAAAAAAGTTTTATCTGGTGTTGACTGGGTGCTGCAATCGGTGCTGCTGGATTTCTGAGTTCATACTTTTTTCTTTTCCAGGTGAACGAAGTGCACGGTCAGTTTGTTGCAGCATTGCAGGATCTTTTCGAAAGGCACAAGGAACGGGTTGGTCACGCCAACCTTCAGTTAAAAATTATTTGATCAGACACTATTTTTTTTGTTTTCCCTATTCATTTTCTGGGAAAGTTAGTGTTAACTTGTTTTGCCTTTGGTATATAGGCTCATTTGTAATTTTGGAATCAATCTTTAATAAAATTCAAATGAATCCTAAAAAAGCGCTTGAATGTTTTTTTTCAGGAATCACTTCAAGTGCTTTGAAATTCAATTTTTCTTCTTTTCTAAAAGTGTTTTTAATTATTCTAAAAGTACTTTTAGATGACGCGCTCTCTAAATTTATTCATGCGTTCTCAATAAAATTAGTGATTATACACAGTAATAAAGACTATTTCATTTTTCAATTAATTGTTTCTCTCATTAGTCCCCACTTGATTGCCTACACAAAATTATTATGCGATTCTAAAATATGGAAAGAGATCATATCTGGATCTCTTTCACTAAATCTACACAATCAATTAATCCAGATTCTTGAAATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004144 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005811 GO:0006629 GO:0006638 GO:0006639 GO:0006641 GO:0006996 GO:0008150 GO:0008152 GO:0008374 GO:0008610 GO:0009058 GO:0009987 GO:0012505 GO:0016020 GO:0016043 GO:0016411 GO:0016740 GO:0016746 GO:0016747 GO:0019432 GO:0031984 GO:0034389 GO:0042175 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045017 GO:0046460 GO:0046463 GO:0046486 GO:0071704 GO:0071840 GO:0098827 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00561 ko04975 map00561 map04975

Pfam Domains

Protein Families

DAGAT

Protein Analysis

1221

Amino Acids

140.77

Weight (kDa)

9.78

Isoelectric Point (pI)

40.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DAGAT	PF03982	319 - 429	9.1e-08	Diacylglycerol acyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001615)

Species	Orthologous Gene IDs
malus_domestica	MD00G1174900.v1.1 MD01G1174900.v1.1
pyrus_communis	pycom01g02540 pycom02g08640 pycom02g21750 pycom05g02950 pycom05g07810 pycom07g08030 pycom11g21700 pycom13g23410 pycom16g13490 pycom17g16180
rosa_chinensis	RchiOBHm_Chr6g0247261 RchiOBHm_Chr7g0204481
rosa_laevigata	RLG00000007852 RLG00000013807 RLG00000018815 RLG00000023656 RLG00000036145 RLG00000036151
rosa_multiflora	Rmu_sc0000367.1_g000212
rosa_roxburghii	Rroxscaffold_1G00054610 Rroxscaffold_3G00223650 Rroxscaffold_4G00310010 Rroxscaffold_4G00326510 Rroxscaffold_5G00347060 Rroxscaffold_7G00200030
rosa_rugosa	Rorug02G0466400 Rorug04G0195600 Rorug06G0200000
rosa_samantha	Rh2DG335000 Rh4BG049400 Rh4CG056000 Rh4CG286000 Rh4DG269000 Rh6AG158900 Rh6AG260100 Rh6BG163100 Rh6BG263200 Rh6CG155300 Rh6CG155400 Rh6CG262200 Rh6CG325100 Rh6DG138200 Rh6DG148900 Rh6DG254100 Rh6DG254400 Rh6DG310900 Rh7DG185300
rosa_wichuraiana	Rw1G006920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	146, 2972
AasI	GACNNNNNNGTC	1 cut(s)	891
Acc36I	ACCTGC	1 cut(s)	764
AccB7I	CCANNNNNTGG	1 cut(s)	1427
AccBSI	CCGCTC	1 cut(s)	449
AccI	GTMKAC	1 cut(s)	1701
AccII	CGCG	2 cut(s)	1061, 3449
AclI	AACGTT	1 cut(s)	1848
AclWI	GGATC	2 cut(s)	3154, 3619
AcoI	YGGCCR	1 cut(s)	1182
AcuI	CTGAAG	1 cut(s)	3174
AcvI	CACGTG	1 cut(s)	2041
AdeI	CACNNNGTG	3 cut(s)	510, 2044, 2210
AfaI	GTAC	9 cut(s)	327, 370, 410, 1322, 1645, 2019, 2822, 3018, 3435
AfeI	AGCGCT	1 cut(s)	3345
AfiI	CCNNNNNNNGG	3 cut(s)	1427, 2133, 2616
AgeI	ACCGGT	1 cut(s)	2126
AjiI	CACGTC	2 cut(s)	2497, 2733
AjnI	CCWGG	6 cut(s)	312, 1240, 1273, 1426, 2175, 3103
AjuI	GAANNNNNNNTTGG	2 cut(s)	2116, 2148
AluBI	AGCT	5 cut(s)	1143, 1371, 1751, 2275, 2977
AluI	AGCT	5 cut(s)	1143, 1371, 1751, 2275, 2977
Alw21I	GWGCWC	4 cut(s)	502, 1314, 1753, 3121
Alw26I	GTCTC	6 cut(s)	332, 556, 1119, 1250, 2318, 2499
Alw44I	GTGCAC	2 cut(s)	498, 3117
AlwI	GGATC	2 cut(s)	3154, 3619
AlwNI	CAGNNNCTG	1 cut(s)	1819
Aor51HI	AGCGCT	1 cut(s)	3345
AoxI	GGCC	5 cut(s)	1182, 1418, 1608, 1715, 2305
ApaLI	GTGCAC	2 cut(s)	498, 3117
ApeKI	GCWGC	5 cut(s)	2004, 2171, 3057, 3069, 3135
AseI	ATTAAT	5 cut(s)	248, 627, 665, 3527, 3641
AsiGI	ACCGGT	1 cut(s)	2126
Asp700I	GAANNNNTTC	1 cut(s)	3370
AspLEI	GCGC	3 cut(s)	1061, 3346, 3451
AspS9I	GGNCC	6 cut(s)	1418, 1556, 1608, 2136, 2512, 2715
AsuC2I	CCSGG	1 cut(s)	1018
AsuHPI	GGTGA	5 cut(s)	1024, 1170, 1450, 2232, 3119
AsuII	TTCGAA	1 cut(s)	3154
AvaII	GGWCC	4 cut(s)	1556, 2136, 2512, 2715
BaeGI	GKGCMC	2 cut(s)	502, 3121
BaeI	ACNNNNGTAYC	4 cut(s)	400, 433, 2001, 2034
BanII	GRGCYC	1 cut(s)	1753
BarI	GAAGNNNNNNTAC	2 cut(s)	1858, 1890
BbrPI	CACGTG	1 cut(s)	2041
BbsI	GAAGAC	1 cut(s)	2257
Bbv12I	GWGCWC	4 cut(s)	502, 1314, 1753, 3121
BbvI	GCAGC	5 cut(s)	1991, 2158, 3044, 3056, 3147
BccI	CCATC	2 cut(s)	1409, 1429
BceAI	ACGGC	1 cut(s)	1595
BcgI	CGANNNNNNTGC	4 cut(s)	868, 902, 2978, 3012
BciT130I	CCWGG	6 cut(s)	314, 1242, 1275, 1428, 2177, 3105
BclI	TGATCA	2 cut(s)	193, 3207
BcnI	CCSGG	1 cut(s)	1018
BcoDI	GTCTC	6 cut(s)	332, 556, 1119, 1250, 2318, 2499
BfaI	CTAG	7 cut(s)	483, 890, 1472, 1679, 1746, 2232, 2544
BfmI	CTRYAG	5 cut(s)	1811, 2005, 2023, 2344, 2939
BfoI	RGCGCY	1 cut(s)	3347
BfuAI	ACCTGC	1 cut(s)	764
BisI	GCNGC	6 cut(s)	2005, 2172, 2553, 3058, 3070, 3136
BlsI	GCNGC	6 cut(s)	2006, 2173, 2554, 3059, 3071, 3137
BmcAI	AGTACT	1 cut(s)	3435
Bme1390I	CCNGG	7 cut(s)	314, 1018, 1242, 1275, 1428, 2177, 3105
Bme18I	GGWCC	4 cut(s)	1556, 2136, 2512, 2715
BmgBI	CACGTC	2 cut(s)	2497, 2733
BmgT120I	GGNCC	6 cut(s)	1418, 1556, 1608, 2136, 2512, 2715
BmiI	GGNNCC	4 cut(s)	1272, 1432, 2513, 2717
BmrFI	CCNGG	7 cut(s)	314, 1018, 1242, 1275, 1428, 2177, 3105
BmrI	ACTGGG	3 cut(s)	701, 1760, 3060
BmsI	GCATC	6 cut(s)	1239, 1592, 2333, 2349, 2448, 2564
BmuI	ACTGGG	3 cut(s)	701, 1760, 3060
BpiI	GAAGAC	1 cut(s)	2257
BplI	GAGNNNNNCTC	2 cut(s)	2271, 2303
Bpu14I	TTCGAA	1 cut(s)	3154
BpuEI	CTTGAG	2 cut(s)	1739, 2051
BpuMI	CCSGG	1 cut(s)	1018
Bsa29I	ATCGAT	1 cut(s)	2061
BsaAI	YACGTR	1 cut(s)	2041
BsaBI	GATNNNNATC	3 cut(s)	198, 2912, 2924
BsaJI	CCNNGG	6 cut(s)	704, 1408, 1426, 2088, 2175, 2861
BsaWI	WCCGGW	2 cut(s)	2126, 3019
BsaXI	ACNNNNNCTCC	6 cut(s)	1705, 1735, 2113, 2143, 2329, 2359
Bsc4I	CCNNNNNNNGG	3 cut(s)	1427, 2133, 2616
Bse118I	RCCGGY	3 cut(s)	1180, 1217, 2126
Bse1I	ACTGG	7 cut(s)	696, 722, 1434, 1509, 1549, 1766, 3055
Bse3DI	GCAATG	2 cut(s)	1689, 3138
Bse8I	GATNNNNATC	3 cut(s)	198, 2912, 2924
BseBI	CCWGG	6 cut(s)	314, 1242, 1275, 1428, 2177, 3105
BseCI	ATCGAT	1 cut(s)	2061
BseDI	CCNNGG	6 cut(s)	704, 1408, 1426, 2088, 2175, 2861
BseGI	GGATG	4 cut(s)	1421, 2029, 2439, 2555
BseJI	GATNNNNATC	3 cut(s)	198, 2912, 2924
BseLI	CCNNNNNNNGG	3 cut(s)	1427, 2133, 2616
BseMI	GCAATG	2 cut(s)	1689, 3138
BseMII	CTCAG	3 cut(s)	1272, 1971, 3072
BseNI	ACTGG	7 cut(s)	696, 722, 1434, 1509, 1549, 1766, 3055
BseRI	GAGGAG	4 cut(s)	2276, 2589, 2656, 2659
BseSI	GKGCMC	2 cut(s)	502, 3121
BseXI	GCAGC	5 cut(s)	1991, 2158, 3044, 3056, 3147
Bsh1236I	CGCG	2 cut(s)	1061, 3449
BshFI	GGCC	5 cut(s)	1184, 1420, 1610, 1717, 2307
BshTI	ACCGGT	1 cut(s)	2126
BshVI	ATCGAT	1 cut(s)	2061
BsiHKAI	GWGCWC	4 cut(s)	502, 1314, 1753, 3121
BsiSI	CCGG	5 cut(s)	1017, 1181, 1218, 2127, 3020
BslFI	GGGAC	4 cut(s)	2083, 2525, 2701, 3531
BslI	CCNNNNNNNGG	3 cut(s)	1427, 2133, 2616
BsmAI	GTCTC	6 cut(s)	332, 556, 1119, 1250, 2318, 2499
BsmBI	CGTCTC	2 cut(s)	332, 2318
BsmFI	GGGAC	4 cut(s)	2083, 2525, 2701, 3531
BsnI	GGCC	5 cut(s)	1184, 1420, 1610, 1717, 2307
Bsp119I	TTCGAA	1 cut(s)	3154
Bsp1286I	GDGCHC	4 cut(s)	502, 1314, 1753, 3121
Bsp143I	GATC	7 cut(s)	193, 303, 2062, 3146, 3207, 3600, 3611
Bsp19I	CCATGG	2 cut(s)	1408, 2088
BspANI	GGCC	5 cut(s)	1184, 1420, 1610, 1717, 2307
BspCNI	CTCAG	3 cut(s)	1273, 1970, 3073
BspDI	ATCGAT	1 cut(s)	2061
BspFNI	CGCG	2 cut(s)	1061, 3449
BspLI	GGNNCC	4 cut(s)	1272, 1432, 2513, 2717
BspMAI	CTGCAG	2 cut(s)	1815, 2009
BspMI	ACCTGC	1 cut(s)	764
BspPI	GGATC	2 cut(s)	3154, 3619
BspT104I	TTCGAA	1 cut(s)	3154
BsrBI	CCGCTC	1 cut(s)	449
BsrDI	GCAATG	2 cut(s)	1689, 3138
BsrFI	RCCGGY	3 cut(s)	1180, 1217, 2126
BsrI	ACTGG	7 cut(s)	696, 722, 1434, 1509, 1549, 1766, 3055
BssAI	RCCGGY	3 cut(s)	1180, 1217, 2126
BssECI	CCNNGG	6 cut(s)	704, 1408, 1426, 2088, 2175, 2861
BssMI	GATC	7 cut(s)	193, 303, 2062, 3146, 3207, 3600, 3611
BssNAI	GTATAC	1 cut(s)	1702
BssT1I	CCWWGG	2 cut(s)	1408, 2088
Bst1107I	GTATAC	1 cut(s)	1702
Bst2UI	CCWGG	6 cut(s)	314, 1242, 1275, 1428, 2177, 3105
Bst4CI	ACNGT	9 cut(s)	343, 708, 1968, 1977, 2027, 2140, 2399, 3123, 3500
Bst6I	CTCTTC	3 cut(s)	554, 2088, 2677
BstAPI	GCANNNNNTGC	1 cut(s)	3066
BstBAI	YACGTR	1 cut(s)	2041
BstBI	TTCGAA	1 cut(s)	3154
BstC8I	GCNNGC	1 cut(s)	423
BstDEI	CTNAG	5 cut(s)	240, 1281, 1957, 2207, 3081
BstDSI	CCRYGG	3 cut(s)	704, 1408, 2088
BstF5I	GGATG	4 cut(s)	1421, 2029, 2439, 2555
BstFNI	CGCG	2 cut(s)	1061, 3449
BstH2I	RGCGCY	1 cut(s)	3347
BstHHI	GCGC	3 cut(s)	1061, 3346, 3451
BstKTI	GATC	7 cut(s)	196, 306, 2065, 3149, 3210, 3603, 3614
BstMAI	GTCTC	6 cut(s)	332, 556, 1119, 1250, 2318, 2499
BstMBI	GATC	7 cut(s)	193, 303, 2062, 3146, 3207, 3600, 3611
BstMWI	GCNNNNNNNGC	6 cut(s)	289, 1058, 1083, 2498, 2735, 3066
BstNI	CCWGG	6 cut(s)	314, 1242, 1275, 1428, 2177, 3105
BstNSI	RCATGY	4 cut(s)	425, 1670, 2956, 2999
BstSCI	CCNGG	7 cut(s)	312, 1016, 1240, 1273, 1426, 2175, 3103
BstSFI	CTRYAG	5 cut(s)	1811, 2005, 2023, 2344, 2939
BstSLI	GKGCMC	2 cut(s)	502, 3121
BstUI	CGCG	2 cut(s)	1061, 3449
BstV1I	GCAGC	5 cut(s)	1991, 2158, 3044, 3056, 3147
BstV2I	GAAGAC	1 cut(s)	2257
BstX2I	RGATCY	2 cut(s)	3146, 3611
BstXI	CCANNNNNNTGG	1 cut(s)	1415
BstYI	RGATCY	2 cut(s)	3146, 3611
BstZ17I	GTATAC	1 cut(s)	1702
Bsu15I	ATCGAT	1 cut(s)	2061
BsuRI	GGCC	5 cut(s)	1184, 1420, 1610, 1717, 2307
BsuTUI	ATCGAT	1 cut(s)	2061
BtgI	CCRYGG	3 cut(s)	704, 1408, 2088
BtrI	CACGTC	2 cut(s)	2497, 2733
BtsCI	GGATG	4 cut(s)	1421, 2029, 2439, 2555
BtsIMutI	CAGTG	2 cut(s)	285, 1278
BveI	ACCTGC	1 cut(s)	764
Cac8I	GCNNGC	1 cut(s)	423
CaiI	CAGNNNCTG	1 cut(s)	1819
CfoI	GCGC	3 cut(s)	1061, 3346, 3451
Cfr10I	RCCGGY	3 cut(s)	1180, 1217, 2126
Cfr13I	GGNCC	6 cut(s)	1418, 1556, 1608, 2136, 2512, 2715
ClaI	ATCGAT	1 cut(s)	2061
CseI	GACGC	1 cut(s)	3455
Csp6I	GTAC	9 cut(s)	326, 369, 409, 1321, 1644, 2018, 2821, 3017, 3434
CspAI	ACCGGT	1 cut(s)	2126
CspCI	CAANNNNNGTGG	4 cut(s)	2022, 2057, 2279, 2314
CviQI	GTAC	9 cut(s)	326, 369, 409, 1321, 1644, 2018, 2821, 3017, 3434
DdeI	CTNAG	5 cut(s)	240, 1281, 1957, 2207, 3081
DpnI	GATC	7 cut(s)	195, 305, 2064, 3148, 3209, 3602, 3613
DpnII	GATC	7 cut(s)	193, 303, 2062, 3146, 3207, 3600, 3611
DraI	TTTAAA	4 cut(s)	180, 750, 2418, 3012
DraIII	CACNNNGTG	3 cut(s)	510, 2044, 2210
DrdI	GACNNNNNNGTC	1 cut(s)	891
DseDI	GACNNNNNNGTC	1 cut(s)	891
EaeI	YGGCCR	1 cut(s)	1182
Eam1104I	CTCTTC	3 cut(s)	554, 2088, 2677
EarI	CTCTTC	3 cut(s)	554, 2088, 2677
EciI	GGCGGA	1 cut(s)	1204
Ecl136II	GAGCTC	1 cut(s)	1751
Eco130I	CCWWGG	2 cut(s)	1408, 2088
Eco24I	GRGCYC	1 cut(s)	1753
Eco32I	GATATC	2 cut(s)	36, 2868
Eco47I	GGWCC	4 cut(s)	1556, 2136, 2512, 2715
Eco47III	AGCGCT	1 cut(s)	3345
Eco53kI	GAGCTC	1 cut(s)	1751
Eco57I	CTGAAG	1 cut(s)	3174
Eco72I	CACGTG	1 cut(s)	2041
EcoICRI	GAGCTC	1 cut(s)	1751
EcoRII	CCWGG	6 cut(s)	312, 1240, 1273, 1426, 2175, 3103
EcoRV	GATATC	2 cut(s)	36, 2868
EcoT14I	CCWWGG	2 cut(s)	1408, 2088
EcoT22I	ATGCAT	1 cut(s)	1668
EcoT38I	GRGCYC	1 cut(s)	1753
ErhI	CCWWGG	2 cut(s)	1408, 2088
Esp3I	CGTCTC	2 cut(s)	332, 2318
FaqI	GGGAC	4 cut(s)	2083, 2525, 2701, 3531
FauI	CCCGC	1 cut(s)	1245
FauNDI	CATATG	2 cut(s)	413, 488
FbaI	TGATCA	2 cut(s)	193, 3207
FblI	GTMKAC	1 cut(s)	1701
Fnu4HI	GCNGC	6 cut(s)	2005, 2172, 2553, 3058, 3070, 3136
FokI	GGATG	4 cut(s)	1408, 2016, 2426, 2542
FriOI	GRGCYC	1 cut(s)	1753
Fsp4HI	GCNGC	6 cut(s)	2005, 2172, 2553, 3058, 3070, 3136
FspBI	CTAG	7 cut(s)	483, 890, 1472, 1679, 1746, 2232, 2544
GlaI	GCGC	3 cut(s)	1060, 3345, 3450
GluI	GCNGC	6 cut(s)	2005, 2172, 2553, 3058, 3070, 3136
HaeII	RGCGCY	1 cut(s)	3347
HaeIII	GGCC	5 cut(s)	1184, 1420, 1610, 1717, 2307
HapII	CCGG	5 cut(s)	1017, 1181, 1218, 2127, 3020
HgaI	GACGC	1 cut(s)	3455
HhaI	GCGC	3 cut(s)	1061, 3346, 3451
Hin6I	GCGC	3 cut(s)	1059, 3344, 3449
HinP1I	GCGC	3 cut(s)	1059, 3344, 3449
HincII	GTYRAC	3 cut(s)	648, 3048, 3262
HindII	GTYRAC	3 cut(s)	648, 3048, 3262
HindIII	AAGCTT	1 cut(s)	2975
HpaI	GTTAAC	1 cut(s)	3262
HpaII	CCGG	5 cut(s)	1017, 1181, 1218, 2127, 3020
HphI	GGTGA	5 cut(s)	1024, 1170, 1450, 2232, 3119
Hpy188I	TCNGA	7 cut(s)	838, 1362, 2051, 2279, 2865, 3082, 3212
HpyAV	CCTTC	9 cut(s)	542, 728, 1131, 2583, 2595, 2613, 2620, 2692, 3198
HpyCH4III	ACNGT	9 cut(s)	343, 708, 1968, 1977, 2027, 2140, 2399, 3123, 3500
HpyCH4IV	ACGT	6 cut(s)	1616, 1848, 2040, 2237, 2496, 2732
HpyF10VI	GCNNNNNNNGC	6 cut(s)	289, 1058, 1083, 2498, 2735, 3066
HpyF3I	CTNAG	5 cut(s)	240, 1281, 1957, 2207, 3081
HpySE526I	ACGT	6 cut(s)	1616, 1848, 2040, 2237, 2496, 2732
HspAI	GCGC	3 cut(s)	1059, 3344, 3449
Ksp22I	TGATCA	2 cut(s)	193, 3207
KspAI	GTTAAC	1 cut(s)	3262
Kzo9I	GATC	7 cut(s)	193, 303, 2062, 3146, 3207, 3600, 3611
LmnI	GCTCC	4 cut(s)	289, 1270, 2280, 2337
Lsp1109I	GCAGC	5 cut(s)	1991, 2158, 3044, 3056, 3147
LweI	GCATC	6 cut(s)	1239, 1592, 2333, 2349, 2448, 2564
MaeI	CTAG	7 cut(s)	483, 890, 1472, 1679, 1746, 2232, 2544
MaeII	ACGT	6 cut(s)	1616, 1848, 2040, 2237, 2496, 2732
MalI	GATC	7 cut(s)	195, 305, 2064, 3148, 3209, 3602, 3613
MbiI	CCGCTC	1 cut(s)	449
MboI	GATC	7 cut(s)	193, 303, 2062, 3146, 3207, 3600, 3611
MfeI	CAATTG	3 cut(s)	987, 1388, 2891
MflI	RGATCY	2 cut(s)	3146, 3611
MhlI	GDGCHC	4 cut(s)	502, 1314, 1753, 3121
MlyI	GAGTC	4 cut(s)	898, 2105, 2289, 2716
MmeI	TCCRAC	1 cut(s)	2029
Mph1103I	ATGCAT	1 cut(s)	1668
MroXI	GAANNNNTTC	1 cut(s)	3370
MslI	CAYNNNNRTG	5 cut(s)	426, 1235, 1407, 1413, 3000
MspA1I	CMGCKG	3 cut(s)	1371, 1840, 2004
MspI	CCGG	5 cut(s)	1017, 1181, 1218, 2127, 3020
MspR9I	CCNGG	7 cut(s)	314, 1018, 1242, 1275, 1428, 2177, 3105
MunI	CAATTG	3 cut(s)	987, 1388, 2891
MvaI	CCWGG	6 cut(s)	314, 1242, 1275, 1428, 2177, 3105
MvnI	CGCG	2 cut(s)	1061, 3449
MwoI	GCNNNNNNNGC	6 cut(s)	289, 1058, 1083, 2498, 2735, 3066
NciI	CCSGG	1 cut(s)	1018
NcoI	CCATGG	2 cut(s)	1408, 2088
NdeI	CATATG	2 cut(s)	413, 488
NdeII	GATC	7 cut(s)	193, 303, 2062, 3146, 3207, 3600, 3611
NlaIV	GGNNCC	4 cut(s)	1272, 1432, 2513, 2717
NmuCI	GTSAC	6 cut(s)	442, 901, 2238, 2453, 2784, 3177
NsiI	ATGCAT	1 cut(s)	1668
NspI	RCATGY	4 cut(s)	425, 1670, 2956, 2999
NspV	TTCGAA	1 cut(s)	3154
PaeI	GCATGC	1 cut(s)	425
PcsI	WCGNNNNNNNCGW	1 cut(s)	870
PdmI	GAANNNNTTC	1 cut(s)	3370
PfeI	GAWTC	9 cut(s)	1531, 2227, 2908, 2920, 3305, 3331, 3366, 3581, 3649
PflFI	GACNNNGTC	2 cut(s)	2459, 2782
PflMI	CCANNNNNTGG	1 cut(s)	1427
PinAI	ACCGGT	1 cut(s)	2126
PkrI	GCNGC	6 cut(s)	2006, 2173, 2554, 3059, 3071, 3137
PleI	GAGTC	4 cut(s)	898, 2104, 2288, 2716
PmaCI	CACGTG	1 cut(s)	2041
PmlI	CACGTG	1 cut(s)	2041
PpsI	GAGTC	4 cut(s)	898, 2104, 2288, 2716
Ppu21I	YACGTR	1 cut(s)	2041
PshBI	ATTAAT	5 cut(s)	248, 627, 665, 3527, 3641
PsiI	TTATAA	2 cut(s)	146, 2972
Psp124BI	GAGCTC	1 cut(s)	1753
Psp1406I	AACGTT	1 cut(s)	1848
Psp6I	CCWGG	6 cut(s)	312, 1240, 1273, 1426, 2175, 3103
PspCI	CACGTG	1 cut(s)	2041
PspGI	CCWGG	6 cut(s)	312, 1240, 1273, 1426, 2175, 3103
PspN4I	GGNNCC	4 cut(s)	1272, 1432, 2513, 2717
PspPI	GGNCC	6 cut(s)	1418, 1556, 1608, 2136, 2512, 2715
PstI	CTGCAG	2 cut(s)	1815, 2009
PstNI	CAGNNNCTG	1 cut(s)	1819
PsuI	RGATCY	2 cut(s)	3146, 3611
PsyI	GACNNNGTC	2 cut(s)	2459, 2782
PvuII	CAGCTG	1 cut(s)	1371
RsaI	GTAC	9 cut(s)	327, 370, 410, 1322, 1645, 2019, 2822, 3018, 3435
RsaNI	GTAC	9 cut(s)	326, 369, 409, 1321, 1644, 2018, 2821, 3017, 3434
RseI	CAYNNNNRTG	5 cut(s)	426, 1235, 1407, 1413, 3000
SacI	GAGCTC	1 cut(s)	1753
SatI	GCNGC	6 cut(s)	2005, 2172, 2553, 3058, 3070, 3136
Sau3AI	GATC	7 cut(s)	193, 303, 2062, 3146, 3207, 3600, 3611
Sau96I	GGNCC	6 cut(s)	1418, 1556, 1608, 2136, 2512, 2715
ScaI	AGTACT	1 cut(s)	3435
SchI	GAGTC	4 cut(s)	898, 2105, 2289, 2716
ScrFI	CCNGG	7 cut(s)	314, 1018, 1242, 1275, 1428, 2177, 3105
SduI	GDGCHC	4 cut(s)	502, 1314, 1753, 3121
SfaNI	GCATC	6 cut(s)	1239, 1592, 2333, 2349, 2448, 2564
SfcI	CTRYAG	5 cut(s)	1811, 2005, 2023, 2344, 2939
SfuI	TTCGAA	1 cut(s)	3154
SinI	GGWCC	4 cut(s)	1556, 2136, 2512, 2715
SmiMI	CAYNNNNRTG	5 cut(s)	426, 1235, 1407, 1413, 3000
SmlI	CTYRAG	2 cut(s)	1754, 2066
SmoI	CTYRAG	2 cut(s)	1754, 2066
SphI	GCATGC	1 cut(s)	425
SspI	AATATT	2 cut(s)	658, 1785
SspMI	CTAG	7 cut(s)	483, 890, 1472, 1679, 1746, 2232, 2544
SstI	GAGCTC	1 cut(s)	1753
StyD4I	CCNGG	7 cut(s)	312, 1016, 1240, 1273, 1426, 2175, 3103
StyI	CCWWGG	2 cut(s)	1408, 2088
TaaI	ACNGT	9 cut(s)	343, 708, 1968, 1977, 2027, 2140, 2399, 3123, 3500
TaiI	ACGT	6 cut(s)	1619, 1851, 2043, 2240, 2499, 2735
TaqI	TCGA	6 cut(s)	727, 1117, 1947, 2061, 2906, 3154
TatI	WGTACW	4 cut(s)	368, 1643, 2820, 3433
TauI	GCSGC	1 cut(s)	2555
TfiI	GAWTC	9 cut(s)	1531, 2227, 2908, 2920, 3305, 3331, 3366, 3581, 3649
TscAI	CASTG	2 cut(s)	285, 1285
TseFI	GTSAC	6 cut(s)	442, 901, 2238, 2453, 2784, 3177
TseI	GCWGC	5 cut(s)	2004, 2171, 3057, 3069, 3135
Tsp45I	GTSAC	6 cut(s)	442, 901, 2238, 2453, 2784, 3177
TspGWI	ACGGA	4 cut(s)	879, 2300, 2344, 2784
TspRI	CASTG	2 cut(s)	285, 1285
Tth111I	GACNNNGTC	2 cut(s)	2459, 2782
Van91I	CCANNNNNTGG	1 cut(s)	1427
VneI	GTGCAC	2 cut(s)	498, 3117
VpaK11BI	GGWCC	4 cut(s)	1556, 2136, 2512, 2715
VspI	ATTAAT	5 cut(s)	248, 627, 665, 3527, 3641
XceI	RCATGY	4 cut(s)	425, 1670, 2956, 2999
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1773, 2300
XmiI	GTMKAC	1 cut(s)	1701
XmnI	GAANNNNTTC	1 cut(s)	3370
XspI	CTAG	7 cut(s)	483, 890, 1472, 1679, 1746, 2232, 2544
ZrmI	AGTACT	1 cut(s)	3435
Zsp2I	ATGCAT	1 cut(s)	1668

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.