RLG00000007852

Ribosome production factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0001615
Physical Location & Seq
Reverse (-)
21971233 .. 21984552
13320 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007852

Sequence Viewer

Length: 1068 bp
ATGGGAGTCAAGGACAAGAAGAAGAACAAGGAGCTTGCCCAGAGAAATTTGGAGCCTGTTGAAAAGGATAAAAGTTCAGGACCTTCAGAGATGAGAAAGAGAAAAAGAGAGAAGAAAACAATGGTGATTCGAGAAAAGTTGTTCAGATCTCTATTGAAGACGATGAAGCCGCAGATGATCAGAGCTAATTCGAAGTTCAGGGAGAGACTGGAAGTGCTGAAATGGTGCTCAATGAAGATGATTAAAGCTAGGAAAAAGAAAAAGAAGAATCGTGATTCCTTCGAAGGTGGGAAGTTACTGGAAAAGAGAGTGGAAGCTGTTCAAGATGAACCTTGTCATTTTATCTGTATGTATTGGTCTTTTACTTGTTTTCAATGTCATTTTAGCTTTTGTTGGGGTCCTGCTTTTATAGAGGAACTACTTTTGGTGATCCCAAATGCACAGTACTATAAAAGAGGCACTTATGACTTGAAAAAGATTATAGAGTATGCAAATAAAAAGGAATTCACTTCTTTAATTGTTGTTCATACCTATTTCCGGGAACCAGATGCTCTCCTAATTATTGGCTTACCTAATGGACCTACAGCCCATTTTAAGCTCTCAAAGCTTGTTTTACGGAAGGATATCAAGTACCATCTTTCTACTGTATTAAACAACTTTACAACACGCCTTGCTTTTTGTTTACTGATGATCATTAATTTCTATGTGTTCAGGTACATTTTTGAAACCAAAGAGACTAAACAGGCTGAAATAAAGGGCAAAAAGACCAAGGATTCCAAAGCTGACGCTATTACTGAAGCAGACACTATTACTGAAGAATGTGGTCCTCGTTTTACCCTTAAATTGATCAGTTTGCAGCACGGGACATTTGATCCTAGAGGCGGGGAGTTTGAGTGGGTTCACAAGAGATCAATGGAAACTTTGATTGATTGGATTGATAGTGAAGTCAACATGAGCAATACTTATGTACTTTTTCGAACTTATGCTCCTGTGCATTTCAGGATTTTGAATGGTGAAGATGTAGATATAGAGGAAACGGTGCTTCGCACTCCAATATCCAAATCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Brix

Protein Analysis

356

Amino Acids

41.79

Weight (kDa)

9.56

Isoelectric Point (pI)

38.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Brix PF04427 136 - 215 3.9e-09 Brix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 170, 882
AclWI GGATC 2 cut(s) 424, 866
AcsI RAATTY 2 cut(s) 46, 503
AcuI CTGAAG 3 cut(s) 69, 816, 834
AfaI GTAC 4 cut(s) 446, 632, 716, 969
AfiI CCNNNNNNNGG 2 cut(s) 537, 881
AgsI TTSAA 7 cut(s) 62, 157, 323, 374, 472, 725, 1009
AloI GAACNNNNNNTCC 2 cut(s) 970, 1002
AluBI AGCT 8 cut(s) 34, 185, 248, 317, 387, 598, 607, 782
AluI AGCT 8 cut(s) 34, 185, 248, 317, 387, 598, 607, 782
Alw21I GWGCWC 1 cut(s) 230
Alw26I GTCTC 2 cut(s) 199, 728
AlwI GGATC 2 cut(s) 424, 866
ApeKI GCWGC 1 cut(s) 856
ApoI RAATTY 2 cut(s) 46, 503
AseI ATTAAT 1 cut(s) 696
Asp700I GAANNNNTTC 1 cut(s) 318
AspS9I GGNCC 4 cut(s) 80, 398, 578, 824
AsuC2I CCSGG 1 cut(s) 539
AsuHPI GGTGA 3 cut(s) 136, 439, 1025
AsuII TTCGAA 3 cut(s) 191, 282, 976
AvaII GGWCC 4 cut(s) 80, 398, 578, 824
BbsI GAAGAC 1 cut(s) 164
Bbv12I GWGCWC 1 cut(s) 230
BbvI GCAGC 1 cut(s) 868
BccI CCATC 1 cut(s) 642
BclI TGATCA 3 cut(s) 177, 690, 846
BcnI CCSGG 1 cut(s) 539
BcoDI GTCTC 2 cut(s) 199, 728
BfaI CTAG 3 cut(s) 249, 876, 1066
BfmI CTRYAG 1 cut(s) 582
BglII AGATCT 1 cut(s) 146
BisI GCNGC 2 cut(s) 170, 857
BlsI GCNGC 2 cut(s) 171, 858
BmcAI AGTACT 1 cut(s) 446
Bme1390I CCNGG 1 cut(s) 539
Bme18I GGWCC 4 cut(s) 80, 398, 578, 824
BmgT120I GGNCC 4 cut(s) 80, 398, 578, 824
BmiI GGNNCC 3 cut(s) 54, 399, 543
BmrFI CCNGG 1 cut(s) 539
BmsI GCATC 1 cut(s) 538
BpiI GAAGAC 1 cut(s) 164
Bpu14I TTCGAA 3 cut(s) 191, 282, 976
BpuMI CCSGG 1 cut(s) 539
BsaJI CCNNGG 1 cut(s) 768
BsaXI ACNNNNNCTCC 4 cut(s) 878, 908, 970, 1000
Bsc4I CCNNNNNNNGG 2 cut(s) 537, 881
Bse1I ACTGG 2 cut(s) 213, 303
BseDI CCNNGG 1 cut(s) 768
BseLI CCNNNNNNNGG 2 cut(s) 537, 881
BseNI ACTGG 2 cut(s) 213, 303
BseXI GCAGC 1 cut(s) 868
BsiHKAI GWGCWC 1 cut(s) 230
BsiSI CCGG 1 cut(s) 538
BslFI GGGAC 1 cut(s) 877
BslI CCNNNNNNNGG 2 cut(s) 537, 881
BsmAI GTCTC 2 cut(s) 199, 728
BsmFI GGGAC 1 cut(s) 877
Bsp119I TTCGAA 3 cut(s) 191, 282, 976
Bsp1286I GDGCHC 1 cut(s) 230
Bsp143I GATC 7 cut(s) 146, 177, 429, 690, 846, 871, 908
BspACI CCGC 2 cut(s) 170, 882
BspLI GGNNCC 3 cut(s) 54, 399, 543
BspPI GGATC 2 cut(s) 424, 866
BspT104I TTCGAA 3 cut(s) 191, 282, 976
BsrI ACTGG 2 cut(s) 213, 303
BssECI CCNNGG 1 cut(s) 768
BssMI GATC 7 cut(s) 146, 177, 429, 690, 846, 871, 908
BssT1I CCWWGG 1 cut(s) 768
Bst4CI ACNGT 3 cut(s) 444, 646, 1039
BstBI TTCGAA 3 cut(s) 191, 282, 976
BstC8I GCNNGC 1 cut(s) 36
BstKTI GATC 7 cut(s) 149, 180, 432, 693, 849, 874, 911
BstMAI GTCTC 2 cut(s) 199, 728
BstMBI GATC 7 cut(s) 146, 177, 429, 690, 846, 871, 908
BstMWI GCNNNNNNNGC 1 cut(s) 604
BstSCI CCNGG 1 cut(s) 537
BstSFI CTRYAG 1 cut(s) 582
BstV1I GCAGC 1 cut(s) 868
BstV2I GAAGAC 1 cut(s) 164
BstX2I RGATCY 1 cut(s) 146
BstYI RGATCY 1 cut(s) 146
Cac8I GCNNGC 1 cut(s) 36
Cfr13I GGNCC 4 cut(s) 80, 398, 578, 824
CseI GACGC 1 cut(s) 794
Csp6I GTAC 4 cut(s) 445, 631, 715, 968
CviAII CATG 1 cut(s) 952
CviQI GTAC 4 cut(s) 445, 631, 715, 968
DpnI GATC 7 cut(s) 148, 179, 431, 692, 848, 873, 910
DpnII GATC 7 cut(s) 146, 177, 429, 690, 846, 871, 908
Eco130I CCWWGG 1 cut(s) 768
Eco32I GATATC 1 cut(s) 625
Eco47I GGWCC 4 cut(s) 80, 398, 578, 824
Eco57I CTGAAG 3 cut(s) 69, 816, 834
EcoO109I RGGNCCY 2 cut(s) 80, 398
EcoRI GAATTC 1 cut(s) 503
EcoRV GATATC 1 cut(s) 625
EcoT14I CCWWGG 1 cut(s) 768
ErhI CCWWGG 1 cut(s) 768
FaeI CATG 1 cut(s) 955
FalI AAGNNNNNCTT 2 cut(s) 445, 477
FaqI GGGAC 1 cut(s) 877
FatI CATG 1 cut(s) 951
FauI CCCGC 1 cut(s) 875
FbaI TGATCA 3 cut(s) 177, 690, 846
Fnu4HI GCNGC 2 cut(s) 170, 857
Fsp4HI GCNGC 2 cut(s) 170, 857
FspBI CTAG 3 cut(s) 249, 876, 1066
GluI GCNGC 2 cut(s) 170, 857
HapII CCGG 1 cut(s) 538
HgaI GACGC 1 cut(s) 794
Hin1II CATG 1 cut(s) 955
HincII GTYRAC 1 cut(s) 949
HindII GTYRAC 1 cut(s) 949
HindIII AAGCTT 1 cut(s) 605
HinfI GANTC 5 cut(s) 6, 127, 268, 275, 773
HpaII CCGG 1 cut(s) 538
HphI GGTGA 3 cut(s) 136, 439, 1025
Hpy166II GTNNAC 3 cut(s) 683, 901, 949
Hpy188I TCNGA 3 cut(s) 88, 146, 182
Hpy188III TCNNGA 5 cut(s) 78, 131, 272, 323, 1000
Hpy8I GTNNAC 3 cut(s) 683, 901, 949
HpyAV CCTTC 4 cut(s) 93, 278, 289, 613
HpyCH4III ACNGT 3 cut(s) 444, 646, 1039
HpyCH4V TGCA 4 cut(s) 440, 491, 856, 994
HpyF10VI GCNNNNNNNGC 1 cut(s) 604
Hsp92II CATG 1 cut(s) 955
Ksp22I TGATCA 3 cut(s) 177, 690, 846
Kzo9I GATC 7 cut(s) 146, 177, 429, 690, 846, 871, 908
LmnI GCTCC 3 cut(s) 31, 52, 991
Lsp1109I GCAGC 1 cut(s) 868
LweI GCATC 1 cut(s) 538
MaeI CTAG 3 cut(s) 249, 876, 1066
MaeIII GTNAC 1 cut(s) 294
MalI GATC 7 cut(s) 148, 179, 431, 692, 848, 873, 910
MboI GATC 7 cut(s) 146, 177, 429, 690, 846, 871, 908
MboII GAAGA 8 cut(s) 31, 34, 124, 169, 247, 277, 827, 1028
MflI RGATCY 1 cut(s) 146
MhlI GDGCHC 1 cut(s) 230
MluCI AATT 7 cut(s) 46, 187, 503, 516, 558, 697, 842
MlyI GAGTC 1 cut(s) 15
MnlI CCTC 5 cut(s) 406, 449, 837, 872, 1024
MroXI GAANNNNTTC 1 cut(s) 318
MseI TTAA 6 cut(s) 243, 515, 594, 650, 696, 840
MspI CCGG 1 cut(s) 538
MspR9I CCNGG 1 cut(s) 539
MwoI GCNNNNNNNGC 1 cut(s) 604
NciI CCSGG 1 cut(s) 539
NdeII GATC 7 cut(s) 146, 177, 429, 690, 846, 871, 908
NlaIII CATG 1 cut(s) 955
NlaIV GGNNCC 3 cut(s) 54, 399, 543
NspV TTCGAA 3 cut(s) 191, 282, 976
PdmI GAANNNNTTC 1 cut(s) 318
PfeI GAWTC 4 cut(s) 127, 268, 275, 773
PfoI TCCNGGA 1 cut(s) 537
PkrI GCNGC 2 cut(s) 171, 858
PleI GAGTC 1 cut(s) 14
PpsI GAGTC 1 cut(s) 14
PpuMI RGGWCCY 2 cut(s) 80, 398
PshBI ATTAAT 1 cut(s) 696
Psp5II RGGWCCY 2 cut(s) 80, 398
PspN4I GGNNCC 3 cut(s) 54, 399, 543
PspPI GGNCC 4 cut(s) 80, 398, 578, 824
PspPPI RGGWCCY 2 cut(s) 80, 398
PsuI RGATCY 1 cut(s) 146
RsaI GTAC 4 cut(s) 446, 632, 716, 969
RsaNI GTAC 4 cut(s) 445, 631, 715, 968
SaqAI TTAA 6 cut(s) 243, 515, 594, 650, 696, 840
SatI GCNGC 2 cut(s) 170, 857
Sau3AI GATC 7 cut(s) 146, 177, 429, 690, 846, 871, 908
Sau96I GGNCC 4 cut(s) 80, 398, 578, 824
ScaI AGTACT 1 cut(s) 446
SchI GAGTC 1 cut(s) 15
ScrFI CCNGG 1 cut(s) 539
SduI GDGCHC 1 cut(s) 230
SfaNI GCATC 1 cut(s) 538
SfcI CTRYAG 1 cut(s) 582
SfuI TTCGAA 3 cut(s) 191, 282, 976
SinI GGWCC 4 cut(s) 80, 398, 578, 824
Sse9I AATT 7 cut(s) 46, 187, 503, 516, 558, 697, 842
SsiI CCGC 2 cut(s) 170, 882
SspMI CTAG 3 cut(s) 249, 876, 1066
StyD4I CCNGG 1 cut(s) 537
StyI CCWWGG 1 cut(s) 768
TaaI ACNGT 3 cut(s) 444, 646, 1039
TaqI TCGA 4 cut(s) 130, 191, 282, 976
TasI AATT 7 cut(s) 46, 187, 503, 516, 558, 697, 842
TatI WGTACW 2 cut(s) 444, 967
TauI GCSGC 1 cut(s) 172
TfiI GAWTC 4 cut(s) 127, 268, 275, 773
Tru1I TTAA 6 cut(s) 243, 515, 594, 650, 696, 840
Tru9I TTAA 6 cut(s) 243, 515, 594, 650, 696, 840
TseI GCWGC 1 cut(s) 856
TspDTI ATGAA 4 cut(s) 179, 248, 342, 515
TspGWI ACGGA 1 cut(s) 631
VpaK11BI GGWCC 4 cut(s) 80, 398, 578, 824
VspI ATTAAT 1 cut(s) 696
XapI RAATTY 2 cut(s) 46, 503
XmnI GAANNNNTTC 1 cut(s) 318
XspI CTAG 3 cut(s) 249, 876, 1066
ZrmI AGTACT 1 cut(s) 446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.