pycom09g19150

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0000990
Physical Location & Seq
Reverse (-)
19930608 .. 19932831
2224 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g19150.1

Sequence Viewer

Length: 1698 bp
ATGCGAGCTACAATATCAAGAAGGCTTCATCAGGCAATGGATAACATCTGTCAACAACGAGGAACCGAGCGTTTGTTTGAGGACTTCATAGAAGATTTTCTTGATGAATCAGATTTTATGGATTACTTTAAGGCAACCTGGTATCCTAGAACTGGGATGTGGATCTCTGCACTACAAAATCTTCCTCTTGCTAGCCAGGAAACCTCTGCGGCAATGGAGTTCTACCACAACCAACTAAAGCTTCGGTTATTGAACGAGAAGAAACCTAGCGTGTACAAACGAGTTGATTGGTTGGTTGATAAGCTGGGTACAAAAGTGCATTCCTACTTCTGGCTTGATGAATATTCTGAGAAGGATGATTTTGCACGATATTGGAAAGATGAGTGGGCGAGTGGTTTAACATCTTGGCGTAAGGCTTTGAAGATTCCTGACCGCAATATTGTCATAGAAGGTACACGTGCAAAAGTTATTGACGAGCTCGACCAAGACAGGGTTTATGTTGTTTGGAACCCTGGTTCTCAGTTCGGTATTTGCAACTGCCGTTGGGCAGAAATGGGCAACCTGTGTGAACATATACTCAAAGTTATTAATGTTTGCCGGAAAAGGTCGTCTACGCCATCTATCAGCCTTTTGCAGTACCACAAGGCCTTGATTGACATGCTGCACTGCCCACCTCATGATTCTTTGATTCGTGATCATGCTGTTTCTTTAGCAGTATTTGTACAGAAGCAATTAAGTGGACTAGTTAATTTGGAAAGCAACGATACCGCAATGGATGTGGCATTTTTTGCCAATCGAGATCAAGAATCAGTAAATGAGGAAGTATTATCTCTAAATGAGAATAACTGTGGGGATGGAGATGTAGCTGCTGAGAGAACCAAGGGTAAGGTGGCTACTGAATCTAGTAATTTGGTAGCACGTGGAGATGACATATGTAATGAGAGTTATGGAGAAGAAATTACTGGCGATGAGATGGATGTCGACCCGTCATCCATCTGCATTTCCCCACCCGGGTTGCATTCTGTTGACGAGGTTGTGTCTAGCAGTGTCTTCCCCGAAAGCAGACAGAGGTCATTGTTTAACAGAGAGCTTGAAGACCTGCCTTCTGCTGATGATGCCCGCACTAATCCAACCGGATTTGAGGATGACATCTTAAATAGAAATAGCCAAGAAAATACGATGGATGAGGACATGAATATCCCTTCCTCAACAATGGAGTTTGTGGAGCAATGCACAGTAACTCATCCTGATGATCACCACAGCCACGATATTGAACCCGCTGTTATTTGCAAGACATCTGAGGACAATACTGTGTATACAAAGACTGCTCCATCTGCATCTATGCCTGTTGAATCACAAGTGGTCGAAGTGGCTGAAGTTCCAGGTGTCATTTCAGAAAGTGATAGAATGGAAACTGAAAACAAGAATGGAAGCACGAGTAATCACCCATCCTCCGCTGATGACGCTGCCAGTATTGACGGGCCTTGTGAGAACTTGGTAAATGATTCTGATTGTAGTCAGGACTCAAAAGCAGTTGGCAATATGATGGTTGTACAACCAGAAGCACTGATTAAATGCTCGTCAAGATCGAGGTACAACGAGGAACAACTGCCTATTGAGAATGGAGATACTGGAACTGGATCTTCTGAGAACCCATCTTCATTTGCTGAACCTCGTCTTACTAAGAGTGAAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

566

Amino Acids

63.42

Weight (kDa)

4.58

Isoelectric Point (pI)

44.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1107
AccI GTMKAC 3 cut(s) 611, 981, 1316
AciI CCGC 6 cut(s) 209, 433, 768, 1120, 1278, 1455
AclWI GGATC 2 cut(s) 170, 1648
AcuI CTGAAG 1 cut(s) 1395
AcvI CACGTG 2 cut(s) 458, 920
AfaI GTAC 7 cut(s) 275, 310, 454, 638, 723, 1554, 1595
AfiI CCNNNNNNNGG 4 cut(s) 152, 330, 490, 1011
AflIII ACRYGT 1 cut(s) 455
AgsI TTSAA 5 cut(s) 253, 421, 1094, 1274, 1352
AhlI ACTAGT 1 cut(s) 742
AjnI CCWGG 4 cut(s) 137, 195, 511, 1381
AjuI GAANNNNNNNTTGG 2 cut(s) 225, 257
AloI GAACNNNNNNTCC 2 cut(s) 499, 531
AluBI AGCT 6 cut(s) 8, 241, 304, 478, 866, 1090
AluI AGCT 6 cut(s) 8, 241, 304, 478, 866, 1090
Alw21I GWGCWC 1 cut(s) 480
AlwI GGATC 2 cut(s) 170, 1648
Ama87I CYCGRG 1 cut(s) 1010
AoxI GGCC 2 cut(s) 645, 1481
ApeKI GCWGC 3 cut(s) 661, 866, 1466
AseI ATTAAT 1 cut(s) 588
Asp700I GAANNNNTTC 1 cut(s) 96
AspS9I GGNCC 1 cut(s) 1481
AsuC2I CCSGG 2 cut(s) 1011, 1012
AsuHPI GGTGA 2 cut(s) 1247, 1436
AsuNHI GCTAGC 1 cut(s) 191
AvaI CYCGRG 1 cut(s) 1010
BaeI ACNNNNGTAYC 2 cut(s) 300, 333
BanII GRGCYC 1 cut(s) 480
BauI CACGAG 1 cut(s) 1435
BbrPI CACGTG 2 cut(s) 458, 920
BbsI GAAGAC 2 cut(s) 1042, 1101
Bbv12I GWGCWC 1 cut(s) 480
BbvI GCAGC 3 cut(s) 648, 853, 1453
BccI CCATC 9 cut(s) 625, 848, 967, 1001, 1174, 1339, 1456, 1540, 1663
BceAI ACGGC 1 cut(s) 525
BciT130I CCWGG 4 cut(s) 139, 197, 513, 1383
BciVI GTATCC 1 cut(s) 153
BclI TGATCA 2 cut(s) 694, 1252
BcnI CCSGG 2 cut(s) 1011, 1012
BcuI ACTAGT 1 cut(s) 742
BfaI CTAG 6 cut(s) 147, 192, 267, 743, 903, 1041
BfuAI ACCTGC 1 cut(s) 1107
BfuI GTATCC 1 cut(s) 153
BisI GCNGC 4 cut(s) 210, 662, 867, 1467
BlsI GCNGC 4 cut(s) 211, 663, 868, 1468
Bme1390I CCNGG 6 cut(s) 139, 197, 513, 1011, 1012, 1383
BmeT110I CYCGRG 1 cut(s) 1010
BmgT120I GGNCC 1 cut(s) 1481
BmiI GGNNCC 2 cut(s) 64, 509
BmrFI CCNGG 6 cut(s) 139, 197, 513, 1011, 1012, 1383
BmrI ACTGGG 1 cut(s) 162
BmsI GCATC 2 cut(s) 1105, 1346
BmtI GCTAGC 1 cut(s) 195
BmuI ACTGGG 1 cut(s) 162
BoxI GACNNNNGTC 1 cut(s) 1069
BpiI GAAGAC 2 cut(s) 1042, 1101
BpuMI CCSGG 2 cut(s) 1011, 1012
BsaAI YACGTR 2 cut(s) 458, 920
BsaBI GATNNNNATC 1 cut(s) 161
BsaJI CCNNGG 3 cut(s) 511, 879, 1010
BsaWI WCCGGW 1 cut(s) 1133
BsaXI ACNNNNNCTCC 2 cut(s) 1436, 1466
Bsc4I CCNNNNNNNGG 4 cut(s) 152, 330, 490, 1011
Bse1I ACTGG 5 cut(s) 157, 967, 1470, 1636, 1642
Bse3DI GCAATG 4 cut(s) 42, 219, 777, 1235
Bse8I GATNNNNATC 1 cut(s) 161
BseBI CCWGG 4 cut(s) 139, 197, 513, 1383
BseDI CCNNGG 3 cut(s) 511, 879, 1010
BseJI GATNNNNATC 1 cut(s) 161
BseLI CCNNNNNNNGG 4 cut(s) 152, 330, 490, 1011
BseMI GCAATG 4 cut(s) 42, 219, 777, 1235
BseMII CTCAG 5 cut(s) 339, 533, 861, 1290, 1638
BseNI ACTGG 5 cut(s) 157, 967, 1470, 1636, 1642
BseXI GCAGC 3 cut(s) 648, 853, 1453
BseYI CCCAGC 1 cut(s) 304
BsgI GTGCAG 2 cut(s) 153, 647
BshFI GGCC 2 cut(s) 647, 1483
BsiHKAI GWGCWC 1 cut(s) 480
BsiHKCI CYCGRG 1 cut(s) 1010
BsiSI CCGG 3 cut(s) 598, 1011, 1134
BslI CCNNNNNNNGG 4 cut(s) 152, 330, 490, 1011
BsmI GAATGC 2 cut(s) 319, 1018
BsnI GGCC 2 cut(s) 647, 1483
BsoBI CYCGRG 1 cut(s) 1010
Bsp1286I GDGCHC 1 cut(s) 480
Bsp1407I TGTACA 3 cut(s) 273, 721, 1552
Bsp143I GATC 6 cut(s) 162, 694, 799, 1252, 1586, 1640
BspACI CCGC 6 cut(s) 209, 433, 768, 1120, 1278, 1455
BspANI GGCC 2 cut(s) 647, 1483
BspCNI CTCAG 5 cut(s) 340, 532, 862, 1291, 1639
BspHI TCATGA 1 cut(s) 676
BspLI GGNNCC 2 cut(s) 64, 509
BspMI ACCTGC 1 cut(s) 1107
BspOI GCTAGC 1 cut(s) 195
BspPI GGATC 2 cut(s) 170, 1648
BsrDI GCAATG 4 cut(s) 42, 219, 777, 1235
BsrGI TGTACA 3 cut(s) 273, 721, 1552
BsrI ACTGG 5 cut(s) 157, 967, 1470, 1636, 1642
BssECI CCNNGG 3 cut(s) 511, 879, 1010
BssMI GATC 6 cut(s) 162, 694, 799, 1252, 1586, 1640
BssNAI GTATAC 1 cut(s) 1317
BssSI CACGAG 1 cut(s) 1435
BssT1I CCWWGG 1 cut(s) 879
Bst1107I GTATAC 1 cut(s) 1317
Bst2BI CACGAG 1 cut(s) 1435
Bst2UI CCWGG 4 cut(s) 139, 197, 513, 1383
Bst4CI ACNGT 3 cut(s) 848, 1237, 1312
BstAPI GCANNNNNTGC 1 cut(s) 788
BstAUI TGTACA 3 cut(s) 273, 721, 1552
BstBAI YACGTR 2 cut(s) 458, 920
BstC8I GCNNGC 3 cut(s) 6, 193, 1120
BstDEI CTNAG 6 cut(s) 348, 519, 870, 1299, 1647, 1683
BstKTI GATC 6 cut(s) 165, 697, 802, 1255, 1589, 1643
BstMBI GATC 6 cut(s) 162, 694, 799, 1252, 1586, 1640
BstMWI GCNNNNNNNGC 4 cut(s) 788, 1115, 1334, 1463
BstNI CCWGG 4 cut(s) 139, 197, 513, 1383
BstNSI RCATGY 1 cut(s) 661
BstPAI GACNNNNGTC 1 cut(s) 1069
BstSCI CCNGG 6 cut(s) 137, 195, 511, 1009, 1010, 1381
BstV1I GCAGC 3 cut(s) 648, 853, 1453
BstV2I GAAGAC 2 cut(s) 1042, 1101
BstX2I RGATCY 2 cut(s) 162, 1640
BstYI RGATCY 2 cut(s) 162, 1640
BstZ17I GTATAC 1 cut(s) 1317
BsuI GTATCC 1 cut(s) 153
BsuRI GGCC 2 cut(s) 647, 1483
BtgZI GCGATG 1 cut(s) 981
BtsI GCAGTG 2 cut(s) 664, 1051
BtsIMutI CAGTG 3 cut(s) 664, 1051, 1565
BveI ACCTGC 1 cut(s) 1107
Cac8I GCNNGC 3 cut(s) 6, 193, 1120
CciI TCATGA 1 cut(s) 676
Cfr13I GGNCC 1 cut(s) 1481
Cfr9I CCCGGG 1 cut(s) 1010
CseI GACGC 1 cut(s) 1472
CsiI ACCWGGT 1 cut(s) 137
Csp6I GTAC 7 cut(s) 274, 309, 453, 637, 722, 1553, 1594
CspCI CAANNNNNGTGG 4 cut(s) 759, 794, 996, 1031
CviAII CATG 4 cut(s) 658, 677, 698, 1192
CviQI GTAC 7 cut(s) 274, 309, 453, 637, 722, 1553, 1594
DdeI CTNAG 6 cut(s) 348, 519, 870, 1299, 1647, 1683
DpnI GATC 6 cut(s) 164, 696, 801, 1254, 1588, 1642
DpnII GATC 6 cut(s) 162, 694, 799, 1252, 1586, 1640
Ecl136II GAGCTC 1 cut(s) 478
Eco130I CCWWGG 1 cut(s) 879
Eco147I AGGCCT 1 cut(s) 647
Eco24I GRGCYC 1 cut(s) 480
Eco53kI GAGCTC 1 cut(s) 478
Eco57I CTGAAG 1 cut(s) 1395
Eco72I CACGTG 2 cut(s) 458, 920
Eco88I CYCGRG 1 cut(s) 1010
EcoICRI GAGCTC 1 cut(s) 478
EcoRII CCWGG 4 cut(s) 137, 195, 511, 1381
EcoT14I CCWWGG 1 cut(s) 879
EcoT38I GRGCYC 1 cut(s) 480
ErhI CCWWGG 1 cut(s) 879
FaeI CATG 4 cut(s) 661, 680, 701, 1195
FalI AAGNNNNNCTT 2 cut(s) 84, 116
FatI CATG 4 cut(s) 657, 676, 697, 1191
FauI CCCGC 2 cut(s) 1127, 1285
FauNDI CATATG 1 cut(s) 932
FbaI TGATCA 2 cut(s) 694, 1252
FblI GTMKAC 3 cut(s) 611, 981, 1316
Fnu4HI GCNGC 4 cut(s) 210, 662, 867, 1467
FriOI GRGCYC 1 cut(s) 480
Fsp4HI GCNGC 4 cut(s) 210, 662, 867, 1467
FspBI CTAG 6 cut(s) 147, 192, 267, 743, 903, 1041
GluI GCNGC 4 cut(s) 210, 662, 867, 1467
GsaI CCCAGC 1 cut(s) 308
HaeIII GGCC 2 cut(s) 647, 1483
HapII CCGG 3 cut(s) 598, 1011, 1134
HgaI GACGC 1 cut(s) 1472
Hin1II CATG 4 cut(s) 661, 680, 701, 1195
HincII GTYRAC 3 cut(s) 53, 982, 1027
HindII GTYRAC 3 cut(s) 53, 982, 1027
HindIII AAGCTT 1 cut(s) 239
HinfI GANTC 9 cut(s) 107, 424, 680, 688, 806, 899, 1352, 1505, 1523
HpaII CCGG 3 cut(s) 598, 1011, 1134
HphI GGTGA 2 cut(s) 1247, 1436
Hpy166II GTNNAC 9 cut(s) 53, 274, 455, 569, 612, 740, 982, 1027, 1317
Hpy188I TCNGA 6 cut(s) 112, 349, 1300, 1396, 1510, 1648
Hpy8I GTNNAC 9 cut(s) 53, 274, 455, 569, 612, 740, 982, 1027, 1317
HpyAV CCTTC 5 cut(s) 15, 346, 443, 1113, 1212
HpyCH4III ACNGT 3 cut(s) 848, 1237, 1312
HpyCH4IV ACGT 2 cut(s) 457, 919
HpyF10VI GCNNNNNNNGC 4 cut(s) 788, 1115, 1334, 1463
HpyF3I CTNAG 6 cut(s) 348, 519, 870, 1299, 1647, 1683
HpySE526I ACGT 2 cut(s) 457, 919
Hsp92II CATG 4 cut(s) 661, 680, 701, 1195
Ksp22I TGATCA 2 cut(s) 694, 1252
Kzo9I GATC 6 cut(s) 162, 694, 799, 1252, 1586, 1640
LmnI GCTCC 2 cut(s) 1225, 1333
Lsp1109I GCAGC 3 cut(s) 648, 853, 1453
LweI GCATC 2 cut(s) 1105, 1346
MabI ACCWGGT 1 cut(s) 137
MaeI CTAG 6 cut(s) 147, 192, 267, 743, 903, 1041
MaeII ACGT 2 cut(s) 457, 919
MaeIII GTNAC 1 cut(s) 1237
MalI GATC 6 cut(s) 164, 696, 801, 1254, 1588, 1642
MboI GATC 6 cut(s) 162, 694, 799, 1252, 1586, 1640
MboII GAAGA 9 cut(s) 104, 173, 271, 433, 965, 1042, 1106, 1635, 1650
MflI RGATCY 2 cut(s) 162, 1640
MhlI GDGCHC 1 cut(s) 480
MluCI AATT 4 cut(s) 731, 748, 907, 957
MlyI GAGTC 1 cut(s) 1517
MmeI TCCRAC 1 cut(s) 1154
MroXI GAANNNNTTC 1 cut(s) 96
MseI TTAA 8 cut(s) 129, 398, 588, 734, 747, 1080, 1154, 1572
MslI CAYNNNNRTG 1 cut(s) 1248
MspA1I CMGCKG 2 cut(s) 1280, 1457
MspI CCGG 3 cut(s) 598, 1011, 1134
MspR9I CCNGG 6 cut(s) 139, 197, 513, 1011, 1012, 1383
Mva1269I GAATGC 2 cut(s) 319, 1018
MvaI CCWGG 4 cut(s) 139, 197, 513, 1383
MwoI GCNNNNNNNGC 4 cut(s) 788, 1115, 1334, 1463
NciI CCSGG 2 cut(s) 1011, 1012
NdeI CATATG 1 cut(s) 932
NdeII GATC 6 cut(s) 162, 694, 799, 1252, 1586, 1640
NheI GCTAGC 1 cut(s) 191
NlaIII CATG 4 cut(s) 661, 680, 701, 1195
NlaIV GGNNCC 2 cut(s) 64, 509
NspI RCATGY 1 cut(s) 661
PagI TCATGA 1 cut(s) 676
PceI AGGCCT 1 cut(s) 647
PcsI WCGNNNNNNNCGW 1 cut(s) 1586
PctI GAATGC 2 cut(s) 319, 1018
PdmI GAANNNNTTC 1 cut(s) 96
PfeI GAWTC 8 cut(s) 107, 424, 680, 688, 806, 899, 1352, 1505
PkrI GCNGC 4 cut(s) 211, 663, 868, 1468
PleI GAGTC 1 cut(s) 1517
PmaCI CACGTG 2 cut(s) 458, 920
PmlI CACGTG 2 cut(s) 458, 920
PpsI GAGTC 1 cut(s) 1517
Ppu21I YACGTR 2 cut(s) 458, 920
PshAI GACNNNNGTC 1 cut(s) 1069
PshBI ATTAAT 1 cut(s) 588
Psp124BI GAGCTC 1 cut(s) 480
Psp6I CCWGG 4 cut(s) 137, 195, 511, 1381
PspCI CACGTG 2 cut(s) 458, 920
PspFI CCCAGC 1 cut(s) 304
PspGI CCWGG 4 cut(s) 137, 195, 511, 1381
PspN4I GGNNCC 2 cut(s) 64, 509
PspPI GGNCC 1 cut(s) 1481
PsuI RGATCY 2 cut(s) 162, 1640
RsaI GTAC 7 cut(s) 275, 310, 454, 638, 723, 1554, 1595
RsaNI GTAC 7 cut(s) 274, 309, 453, 637, 722, 1553, 1594
RseI CAYNNNNRTG 1 cut(s) 1248
SacI GAGCTC 1 cut(s) 480
SalI GTCGAC 1 cut(s) 980
SaqAI TTAA 8 cut(s) 129, 398, 588, 734, 747, 1080, 1154, 1572
SatI GCNGC 4 cut(s) 210, 662, 867, 1467
Sau3AI GATC 6 cut(s) 162, 694, 799, 1252, 1586, 1640
Sau96I GGNCC 1 cut(s) 1481
SchI GAGTC 1 cut(s) 1517
ScrFI CCNGG 6 cut(s) 139, 197, 513, 1011, 1012, 1383
SduI GDGCHC 1 cut(s) 480
SexAI ACCWGGT 1 cut(s) 137
SfaNI GCATC 2 cut(s) 1105, 1346
SmaI CCCGGG 1 cut(s) 1012
SmiMI CAYNNNNRTG 1 cut(s) 1248
SpeI ACTAGT 1 cut(s) 742
Sse9I AATT 4 cut(s) 731, 748, 907, 957
SseBI AGGCCT 1 cut(s) 647
SsiI CCGC 6 cut(s) 209, 433, 768, 1120, 1278, 1455
SspI AATATT 2 cut(s) 344, 439
SspMI CTAG 6 cut(s) 147, 192, 267, 743, 903, 1041
SstI GAGCTC 1 cut(s) 480
StuI AGGCCT 1 cut(s) 647
StyD4I CCNGG 6 cut(s) 137, 195, 511, 1009, 1010, 1381
StyI CCWWGG 1 cut(s) 879
TaaI ACNGT 3 cut(s) 848, 1237, 1312
TaiI ACGT 2 cut(s) 460, 922
TaqI TCGA 5 cut(s) 480, 796, 981, 1365, 1589
TasI AATT 4 cut(s) 731, 748, 907, 957
TatI WGTACW 3 cut(s) 273, 721, 1552
TauI GCSGC 1 cut(s) 212
TfiI GAWTC 8 cut(s) 107, 424, 680, 688, 806, 899, 1352, 1505
Tru1I TTAA 8 cut(s) 129, 398, 588, 734, 747, 1080, 1154, 1572
Tru9I TTAA 8 cut(s) 129, 398, 588, 734, 747, 1080, 1154, 1572
TscAI CASTG 3 cut(s) 671, 1051, 1572
TseI GCWGC 3 cut(s) 661, 866, 1466
TspDTI ATGAA 6 cut(s) 17, 76, 120, 354, 1208, 1650
TspMI CCCGGG 1 cut(s) 1010
TspRI CASTG 3 cut(s) 671, 1051, 1572
VspI ATTAAT 1 cut(s) 588
XceI RCATGY 1 cut(s) 661
XcmI CCANNNNNNNNNTGG 1 cut(s) 886
XmaI CCCGGG 1 cut(s) 1010
XmiI GTMKAC 3 cut(s) 611, 981, 1316
XmnI GAANNNNTTC 1 cut(s) 96
XspI CTAG 6 cut(s) 147, 192, 267, 743, 903, 1041
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.