Rroxscaffold_3G00218200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000990
Physical Location & Seq
Forward (+)
260283 .. 269303
9021 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00218200.1

Sequence Viewer

Length: 1389 bp
ATGGCTAGATGGGATGAGATTCTGTCTCTTCCTGTACAGAATCCTCCGACATTGGAGTTTTCTGCTGCTGATCTTGTGTGGTCGAAGGTGGAAGGTTGGCGTGACAAGAAAGATAGAGTTGCTGTAATCCCCTATGCCAGAGTTGATGCTTTTGTGAGGGGTGAATCTTCGAACAAAGAGTGTCTGACGAGATTTCATGTTGAAGCGAGGCGGCGGCGGCAGGCAAAGACGGATTGCAAACCAAAGGTTGATGGCATCCTTGAATATATTCTATATTGGTGTTCTTTTGGTCCTGATGACCACAGGATGGGCGGCTCTATACGACCCAGTAGGACTAATTATGTCCCAAAGAAGAAAAATTCTGGTCGACCAAACACCAAGAGAGGTTGTACCTGCCACTTTATGGTGAAACGCTTAATTGCTGAACCGTCAGTAGCACTTCTTGTATATAATTCTGTTAAGCATGTGGATAAGAAAGGGTTGCCATGCCATGGCCCTGAAGATAAGATGGCTGCTGGAACGCGCGCTATGTTTGCCCCATACATCTCTGAGGATCTACGCCTTCGTGTGCTATCTTTGCTATATGTGGGGGTCTCCGTGGAGACCATAATGCAGAGACACACTGAATCTGTAGAGAGACAGGGTGGTCCATGTAATCGTGATGACCTCTTAACCCATAGGTACGTTCGCAGACAGGAGAGGATCATTCGACGTTCTACATATGAATTGGATGCGGATGATGCAGTTAGCATCAGCACGTGGGTGGAAAACCATCAGAATAACATTTTCTTCTATGAGGATTTCTCCGATACGGACCCTTTCACTTTGGGCATTCAAACAGACTGGCAGCTGCAACAGATGATTCGCTTTGGCAATCACAGCCTTATAGCCTCTGATTCAAGGTTCGGAACAAACAAATTAAAGTATCCTGTTCACAGTCTCCTTGTATTTAACCAAGACAACAAGGCCATTCCGGTAGCATGGATAGTGAGTCCAAAATTTGCAAGTTCTGATACCCATAAATGGATGAGAGCTCTTTACAATAGAGTTCAGACGAAAGATCCTTCATGGAAGTTGGCTGGGTTCATAGTGGATGATCCTCAGGCTGATGTACACACTATCAGGGATGTATTTCAGTGCTCAGTGCTGATAAGCTTCTGGCGGGTTCGTCATGCATGGCATAAAAACTTGGTAAAGAAATGTTTGGACGACGAGATGCGAGCTACAATATCAAGAAGGCTTCATCAGGCAGTAGATAACATATGTCAACAACCAGGAACAGATGGTTTGTTTGCGGATTTCATGGAAGATTTTGTTGATGCATCAGATTTTGTGGATTACTTTAAGGCAACCTGGTATCCGAGAATAGGTTGGTCACTATCATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

462

Amino Acids

53.44

Weight (kDa)

8.94

Isoelectric Point (pI)

41.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 645
Acc36I ACCTGC 1 cut(s) 401
AccB7I CCANNNNNTGG 3 cut(s) 307, 403, 491
AccI GTMKAC 1 cut(s) 367
AccII CGCG 2 cut(s) 523, 525
AciI CCGC 7 cut(s) 211, 214, 217, 312, 734, 1162, 1295
AclWI GGATC 4 cut(s) 561, 710, 1055, 1091
AcsI RAATTY 2 cut(s) 358, 998
AcuI CTGAAG 1 cut(s) 519
AcvI CACGTG 1 cut(s) 759
AfaI GTAC 4 cut(s) 36, 391, 683, 1113
AfiI CCNNNNNNNGG 5 cut(s) 307, 403, 491, 1023, 1367
AgsI TTSAA 4 cut(s) 203, 263, 836, 900
AjnI CCWGG 2 cut(s) 1273, 1352
AleI CACNNNNGTG 1 cut(s) 761
AluBI AGCT 4 cut(s) 850, 1034, 1155, 1223
AluI AGCT 4 cut(s) 850, 1034, 1155, 1223
Alw21I GWGCWC 2 cut(s) 1036, 1142
Alw26I GTCTC 6 cut(s) 30, 596, 598, 610, 631, 944
AlwI GGATC 4 cut(s) 561, 710, 1055, 1091
AoxI GGCC 2 cut(s) 493, 966
ApeKI GCWGC 4 cut(s) 65, 512, 847, 850
ApoI RAATTY 2 cut(s) 358, 998
Asp700I GAANNNNTTC 1 cut(s) 267
AspLEI GCGC 2 cut(s) 525, 527
AspS9I GGNCC 4 cut(s) 290, 494, 647, 814
AsuHPI GGTGA 2 cut(s) 173, 418
AsuII TTCGAA 1 cut(s) 170
AvaII GGWCC 3 cut(s) 290, 647, 814
AxyI CCTNAGG 1 cut(s) 1101
BanII GRGCYC 1 cut(s) 1036
BbrPI CACGTG 1 cut(s) 759
Bbv12I GWGCWC 2 cut(s) 1036, 1142
BbvI GCAGC 4 cut(s) 52, 499, 837, 859
BccI CCATC 6 cut(s) 3, 245, 301, 502, 780, 1277
BciT130I CCWGG 2 cut(s) 1275, 1354
BciVI GTATCC 2 cut(s) 936, 1368
BcoDI GTCTC 6 cut(s) 30, 596, 598, 610, 631, 944
BfaI CTAG 1 cut(s) 6
BfmI CTRYAG 1 cut(s) 630
BfuAI ACCTGC 1 cut(s) 401
BfuI GTATCC 2 cut(s) 936, 1368
BisI GCNGC 8 cut(s) 66, 212, 215, 218, 313, 513, 848, 851
BlsI GCNGC 8 cut(s) 67, 213, 216, 219, 314, 514, 849, 852
Bme1390I CCNGG 2 cut(s) 1275, 1354
Bme18I GGWCC 3 cut(s) 290, 647, 814
BmgT120I GGNCC 4 cut(s) 290, 494, 647, 814
BmiI GGNNCC 1 cut(s) 816
BmrFI CCNGG 2 cut(s) 1275, 1354
BmrI ACTGGG 1 cut(s) 321
BmsI GCATC 8 cut(s) 136, 264, 721, 730, 759, 1206, 1309, 1331
BmuI ACTGGG 1 cut(s) 321
BplI GAGNNNNNCTC 2 cut(s) 788, 820
Bpu14I TTCGAA 1 cut(s) 170
BsaAI YACGTR 1 cut(s) 759
BsaI GGTCTC 2 cut(s) 596, 598
BsaJI CCNNGG 2 cut(s) 490, 597
BsaWI WCCGGW 1 cut(s) 973
Bsc4I CCNNNNNNNGG 5 cut(s) 307, 403, 491, 1023, 1367
Bse1I ACTGG 2 cut(s) 327, 848
Bse21I CCTNAGG 1 cut(s) 1101
BseBI CCWGG 2 cut(s) 1275, 1354
BseDI CCNNGG 2 cut(s) 490, 597
BseGI GGATG 8 cut(s) 19, 255, 312, 736, 742, 1032, 1099, 1132
BseLI CCNNNNNNNGG 5 cut(s) 307, 403, 491, 1023, 1367
BseMII CTCAG 3 cut(s) 540, 1115, 1155
BseNI ACTGG 2 cut(s) 327, 848
BsePI GCGCGC 1 cut(s) 523
BseXI GCAGC 4 cut(s) 52, 499, 837, 859
BseYI CCCAGC 1 cut(s) 1079
Bsh1236I CGCG 2 cut(s) 523, 525
BshFI GGCC 2 cut(s) 495, 968
BsiHKAI GWGCWC 2 cut(s) 1036, 1142
BsiSI CCGG 1 cut(s) 974
BslFI GGGAC 1 cut(s) 329
BslI CCNNNNNNNGG 5 cut(s) 307, 403, 491, 1023, 1367
BsmAI GTCTC 6 cut(s) 30, 596, 598, 610, 631, 944
BsmFI GGGAC 1 cut(s) 329
BsmI GAATGC 1 cut(s) 831
BsnI GGCC 2 cut(s) 495, 968
Bso31I GGTCTC 2 cut(s) 596, 598
Bsp119I TTCGAA 1 cut(s) 170
Bsp1286I GDGCHC 2 cut(s) 1036, 1142
Bsp1407I TGTACA 2 cut(s) 34, 1111
Bsp143I GATC 5 cut(s) 70, 553, 702, 1060, 1096
Bsp19I CCATGG 1 cut(s) 490
BspACI CCGC 7 cut(s) 211, 214, 217, 312, 734, 1162, 1295
BspANI GGCC 2 cut(s) 495, 968
BspCNI CTCAG 3 cut(s) 541, 1114, 1154
BspFNI CGCG 2 cut(s) 523, 525
BspLI GGNNCC 1 cut(s) 816
BspMI ACCTGC 1 cut(s) 401
BspPI GGATC 4 cut(s) 561, 710, 1055, 1091
BspT104I TTCGAA 1 cut(s) 170
BspTNI GGTCTC 2 cut(s) 596, 598
BsrGI TGTACA 2 cut(s) 34, 1111
BsrI ACTGG 2 cut(s) 327, 848
BssECI CCNNGG 2 cut(s) 490, 597
BssHII GCGCGC 1 cut(s) 523
BssMI GATC 5 cut(s) 70, 553, 702, 1060, 1096
BssT1I CCWWGG 1 cut(s) 490
Bst2UI CCWGG 2 cut(s) 1275, 1354
Bst4CI ACNGT 2 cut(s) 429, 938
Bst6I CTCTTC 1 cut(s) 33
BstAUI TGTACA 2 cut(s) 34, 1111
BstBAI YACGTR 1 cut(s) 759
BstBI TTCGAA 1 cut(s) 170
BstC8I GCNNGC 3 cut(s) 222, 525, 1221
BstDEI CTNAG 3 cut(s) 549, 1101, 1141
BstDSI CCRYGG 2 cut(s) 490, 597
BstF5I GGATG 8 cut(s) 19, 255, 312, 736, 742, 1032, 1099, 1132
BstFNI CGCG 2 cut(s) 523, 525
BstHHI GCGC 2 cut(s) 525, 527
BstKTI GATC 5 cut(s) 73, 556, 705, 1063, 1099
BstMAI GTCTC 6 cut(s) 30, 596, 598, 610, 631, 944
BstMBI GATC 5 cut(s) 70, 553, 702, 1060, 1096
BstMWI GCNNNNNNNGC 5 cut(s) 217, 533, 577, 740, 879
BstNI CCWGG 2 cut(s) 1275, 1354
BstNSI RCATGY 1 cut(s) 467
BstSCI CCNGG 2 cut(s) 1273, 1352
BstSFI CTRYAG 1 cut(s) 630
BstUI CGCG 2 cut(s) 523, 525
BstV1I GCAGC 4 cut(s) 52, 499, 837, 859
BstX2I RGATCY 2 cut(s) 553, 1060
BstYI RGATCY 2 cut(s) 553, 1060
Bsu36I CCTNAGG 1 cut(s) 1101
BsuI GTATCC 2 cut(s) 936, 1368
BsuRI GGCC 2 cut(s) 495, 968
BtgI CCRYGG 2 cut(s) 490, 597
BtsCI GGATG 8 cut(s) 19, 255, 312, 736, 742, 1032, 1099, 1132
BtsIMutI CAGTG 3 cut(s) 621, 1142, 1149
BveI ACCTGC 1 cut(s) 401
Cac8I GCNNGC 3 cut(s) 222, 525, 1221
CfoI GCGC 2 cut(s) 525, 527
Cfr13I GGNCC 4 cut(s) 290, 494, 647, 814
CsiI ACCWGGT 1 cut(s) 1352
Csp6I GTAC 4 cut(s) 35, 390, 682, 1112
CviQI GTAC 4 cut(s) 35, 390, 682, 1112
DdeI CTNAG 3 cut(s) 549, 1101, 1141
DpnI GATC 5 cut(s) 72, 555, 704, 1062, 1098
DpnII GATC 5 cut(s) 70, 553, 702, 1060, 1096
DrdI GACNNNNNNGTC 1 cut(s) 645
DseDI GACNNNNNNGTC 1 cut(s) 645
Eam1104I CTCTTC 1 cut(s) 33
EarI CTCTTC 1 cut(s) 33
Ecl136II GAGCTC 1 cut(s) 1034
Eco130I CCWWGG 1 cut(s) 490
Eco24I GRGCYC 1 cut(s) 1036
Eco31I GGTCTC 2 cut(s) 596, 598
Eco47I GGWCC 3 cut(s) 290, 647, 814
Eco53kI GAGCTC 1 cut(s) 1034
Eco57I CTGAAG 1 cut(s) 519
Eco72I CACGTG 1 cut(s) 759
Eco81I CCTNAGG 1 cut(s) 1101
EcoICRI GAGCTC 1 cut(s) 1034
EcoRII CCWGG 2 cut(s) 1273, 1352
EcoT14I CCWWGG 1 cut(s) 490
EcoT22I ATGCAT 2 cut(s) 1177, 1324
EcoT38I GRGCYC 1 cut(s) 1036
ErhI CCWWGG 1 cut(s) 490
FaqI GGGAC 1 cut(s) 329
FauI CCCGC 1 cut(s) 1155
FauNDI CATATG 2 cut(s) 721, 1262
FblI GTMKAC 1 cut(s) 367
Fnu4HI GCNGC 8 cut(s) 66, 212, 215, 218, 313, 513, 848, 851
FokI GGATG 8 cut(s) 26, 242, 319, 743, 749, 1039, 1106, 1139
FriOI GRGCYC 1 cut(s) 1036
Fsp4HI GCNGC 8 cut(s) 66, 212, 215, 218, 313, 513, 848, 851
FspBI CTAG 1 cut(s) 6
GlaI GCGC 2 cut(s) 524, 526
GluI GCNGC 8 cut(s) 66, 212, 215, 218, 313, 513, 848, 851
GsaI CCCAGC 1 cut(s) 1083
HaeIII GGCC 2 cut(s) 495, 968
HapII CCGG 1 cut(s) 974
HhaI GCGC 2 cut(s) 525, 527
Hin6I GCGC 2 cut(s) 523, 525
HinP1I GCGC 2 cut(s) 523, 525
HincII GTYRAC 2 cut(s) 368, 1268
HindII GTYRAC 2 cut(s) 368, 1268
HindIII AAGCTT 1 cut(s) 1153
HinfI GANTC 7 cut(s) 19, 40, 164, 626, 862, 896, 991
HpaII CCGG 1 cut(s) 974
HphI GGTGA 2 cut(s) 173, 418
Hpy166II GTNNAC 4 cut(s) 368, 934, 1114, 1268
Hpy188III TCNNGA 3 cut(s) 293, 659, 1233
Hpy8I GTNNAC 4 cut(s) 368, 934, 1114, 1268
Hpy99I CGWCG 2 cut(s) 714, 1214
HpyAV CCTTC 5 cut(s) 79, 86, 572, 1074, 1230
HpyCH4III ACNGT 2 cut(s) 429, 938
HpyCH4IV ACGT 3 cut(s) 684, 712, 758
HpyCH4V TGCA 7 cut(s) 237, 613, 743, 853, 1004, 1175, 1322
HpyF10VI GCNNNNNNNGC 5 cut(s) 217, 533, 577, 740, 879
HpyF3I CTNAG 3 cut(s) 549, 1101, 1141
HpySE526I ACGT 3 cut(s) 684, 712, 758
HspAI GCGC 2 cut(s) 523, 525
Kzo9I GATC 5 cut(s) 70, 553, 702, 1060, 1096
Lsp1109I GCAGC 4 cut(s) 52, 499, 837, 859
LweI GCATC 8 cut(s) 136, 264, 721, 730, 759, 1206, 1309, 1331
MabI ACCWGGT 1 cut(s) 1352
MaeI CTAG 1 cut(s) 6
MaeII ACGT 3 cut(s) 684, 712, 758
MaeIII GTNAC 2 cut(s) 101, 1374
MalI GATC 5 cut(s) 72, 555, 704, 1062, 1098
MboI GATC 5 cut(s) 70, 553, 702, 1060, 1096
MboII GAAGA 6 cut(s) 20, 159, 364, 512, 781, 1319
MflI RGATCY 2 cut(s) 553, 1060
MhlI GDGCHC 2 cut(s) 1036, 1142
MluCI AATT 7 cut(s) 337, 358, 417, 451, 725, 917, 998
MlyI GAGTC 1 cut(s) 1000
MmeI TCCRAC 1 cut(s) 71
Mph1103I ATGCAT 2 cut(s) 1177, 1324
MroXI GAANNNNTTC 1 cut(s) 267
MseI TTAA 7 cut(s) 416, 459, 671, 920, 951, 1344, 1387
MslI CAYNNNNRTG 1 cut(s) 761
MspA1I CMGCKG 1 cut(s) 850
MspI CCGG 1 cut(s) 974
MspR9I CCNGG 2 cut(s) 1275, 1354
Mva1269I GAATGC 1 cut(s) 831
MvaI CCWGG 2 cut(s) 1275, 1354
MvnI CGCG 2 cut(s) 523, 525
MwoI GCNNNNNNNGC 5 cut(s) 217, 533, 577, 740, 879
NcoI CCATGG 1 cut(s) 490
NdeI CATATG 2 cut(s) 721, 1262
NdeII GATC 5 cut(s) 70, 553, 702, 1060, 1096
NlaIV GGNNCC 1 cut(s) 816
NmuCI GTSAC 2 cut(s) 101, 1374
NsiI ATGCAT 2 cut(s) 1177, 1324
NspI RCATGY 1 cut(s) 467
NspV TTCGAA 1 cut(s) 170
OliI CACNNNNGTG 1 cut(s) 761
PauI GCGCGC 1 cut(s) 523
PctI GAATGC 1 cut(s) 831
PdmI GAANNNNTTC 1 cut(s) 267
PfeI GAWTC 6 cut(s) 19, 40, 164, 626, 862, 896
PflMI CCANNNNNTGG 3 cut(s) 307, 403, 491
PkrI GCNGC 8 cut(s) 67, 213, 216, 219, 314, 514, 849, 852
PleI GAGTC 1 cut(s) 999
PmaCI CACGTG 1 cut(s) 759
PmlI CACGTG 1 cut(s) 759
PpsI GAGTC 1 cut(s) 999
Ppu21I YACGTR 1 cut(s) 759
Psp124BI GAGCTC 1 cut(s) 1036
Psp6I CCWGG 2 cut(s) 1273, 1352
PspCI CACGTG 1 cut(s) 759
PspFI CCCAGC 1 cut(s) 1079
PspGI CCWGG 2 cut(s) 1273, 1352
PspN4I GGNNCC 1 cut(s) 816
PspPI GGNCC 4 cut(s) 290, 494, 647, 814
PsuI RGATCY 2 cut(s) 553, 1060
PteI GCGCGC 1 cut(s) 523
PvuII CAGCTG 1 cut(s) 850
RsaI GTAC 4 cut(s) 36, 391, 683, 1113
RsaNI GTAC 4 cut(s) 35, 390, 682, 1112
RseI CAYNNNNRTG 1 cut(s) 761
SacI GAGCTC 1 cut(s) 1036
SalI GTCGAC 1 cut(s) 366
SaqAI TTAA 7 cut(s) 416, 459, 671, 920, 951, 1344, 1387
SatI GCNGC 8 cut(s) 66, 212, 215, 218, 313, 513, 848, 851
Sau3AI GATC 5 cut(s) 70, 553, 702, 1060, 1096
Sau96I GGNCC 4 cut(s) 290, 494, 647, 814
SchI GAGTC 1 cut(s) 1000
ScrFI CCNGG 2 cut(s) 1275, 1354
SduI GDGCHC 2 cut(s) 1036, 1142
SexAI ACCWGGT 1 cut(s) 1352
SfaNI GCATC 8 cut(s) 136, 264, 721, 730, 759, 1206, 1309, 1331
SfcI CTRYAG 1 cut(s) 630
SfuI TTCGAA 1 cut(s) 170
SinI GGWCC 3 cut(s) 290, 647, 814
SmiMI CAYNNNNRTG 1 cut(s) 761
Sse9I AATT 7 cut(s) 337, 358, 417, 451, 725, 917, 998
SsiI CCGC 7 cut(s) 211, 214, 217, 312, 734, 1162, 1295
SspMI CTAG 1 cut(s) 6
SstI GAGCTC 1 cut(s) 1036
StyD4I CCNGG 2 cut(s) 1273, 1352
StyI CCWWGG 1 cut(s) 490
TaaI ACNGT 2 cut(s) 429, 938
TaiI ACGT 3 cut(s) 687, 715, 761
TaqI TCGA 4 cut(s) 83, 170, 367, 709
TasI AATT 7 cut(s) 337, 358, 417, 451, 725, 917, 998
TatI WGTACW 2 cut(s) 34, 1111
TauI GCSGC 4 cut(s) 214, 217, 220, 315
TfiI GAWTC 6 cut(s) 19, 40, 164, 626, 862, 896
Tru1I TTAA 7 cut(s) 416, 459, 671, 920, 951, 1344, 1387
Tru9I TTAA 7 cut(s) 416, 459, 671, 920, 951, 1344, 1387
TscAI CASTG 3 cut(s) 628, 1142, 1149
TseFI GTSAC 2 cut(s) 101, 1374
TseI GCWGC 4 cut(s) 65, 512, 847, 850
Tsp45I GTSAC 2 cut(s) 101, 1374
TspDTI ATGAA 6 cut(s) 185, 738, 1056, 1075, 1232, 1291
TspGWI ACGGA 3 cut(s) 245, 586, 827
TspRI CASTG 3 cut(s) 628, 1142, 1149
Van91I CCANNNNNTGG 3 cut(s) 307, 403, 491
VpaK11BI GGWCC 3 cut(s) 290, 647, 814
XapI RAATTY 2 cut(s) 358, 998
XceI RCATGY 1 cut(s) 467
XmiI GTMKAC 1 cut(s) 367
XmnI GAANNNNTTC 1 cut(s) 267
XspI CTAG 1 cut(s) 6
Zsp2I ATGCAT 2 cut(s) 1177, 1324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.