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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 2727 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 645 |
| Acc36I | ACCTGC | 1 cut(s) | 401 |
| Acc65I | GGTACC | 1 cut(s) | 1523 |
| AccB1I | GGYRCC | 1 cut(s) | 1523 |
| AccB7I | CCANNNNNTGG | 3 cut(s) | 307, 403, 491 |
| AccBSI | CCGCTC | 1 cut(s) | 1608 |
| AccI | GTMKAC | 2 cut(s) | 367, 1488 |
| AccII | CGCG | 3 cut(s) | 523, 525, 1219 |
| AclWI | GGATC | 8 cut(s) | 561, 710, 1091, 1998, 2318, 2393, 2406, 2700 |
| AcsI | RAATTY | 3 cut(s) | 358, 998, 1433 |
| AcuI | CTGAAG | 3 cut(s) | 519, 2322, 2553 |
| AfeI | AGCGCT | 1 cut(s) | 438 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 307, 403, 491, 1023, 1367, 1545 |
| AflIII | ACRYGT | 2 cut(s) | 1418, 1670 |
| AjnI | CCWGG | 4 cut(s) | 1352, 1410, 1860, 2218 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1714, 1746 |
| Alw21I | GWGCWC | 2 cut(s) | 1036, 1142 |
| Alw26I | GTCTC | 8 cut(s) | 30, 596, 598, 610, 631, 803, 944, 1208 |
| AlwI | GGATC | 8 cut(s) | 561, 710, 1091, 1998, 2318, 2393, 2406, 2700 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 2300 |
| Aor51HI | AGCGCT | 1 cut(s) | 438 |
| AoxI | GGCC | 6 cut(s) | 493, 966, 1863, 2035, 2279, 3036 |
| ApaI | GGGCCC | 1 cut(s) | 2039 |
| ApeKI | GCWGC | 7 cut(s) | 65, 847, 850, 1424, 1974, 1997, 3019 |
| ApoI | RAATTY | 3 cut(s) | 358, 998, 1433 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 267 |
| Asp718I | GGTACC | 1 cut(s) | 1523 |
| AspLEI | GCGC | 3 cut(s) | 439, 525, 527 |
| AsuC2I | CCSGG | 2 cut(s) | 1691, 1728 |
| AsuHPI | GGTGA | 3 cut(s) | 173, 418, 1615 |
| AvaII | GGWCC | 5 cut(s) | 290, 647, 814, 1687, 1958 |
| AxyI | CCTNAGG | 2 cut(s) | 1101, 2791 |
| BaeGI | GKGCMC | 1 cut(s) | 2039 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1515, 1548 |
| BamHI | GGATCC | 1 cut(s) | 2398 |
| BanI | GGYRCC | 1 cut(s) | 1523 |
| BanII | GRGCYC | 2 cut(s) | 1036, 2039 |
| BbsI | GAAGAC | 1 cut(s) | 1388 |
| Bbv12I | GWGCWC | 2 cut(s) | 1036, 1142 |
| BbvI | GCAGC | 7 cut(s) | 52, 837, 859, 1436, 1986, 2009, 3006 |
| BceAI | ACGGC | 2 cut(s) | 2471, 2905 |
| BciT130I | CCWGG | 4 cut(s) | 1354, 1412, 1862, 2220 |
| BciVI | GTATCC | 2 cut(s) | 936, 1368 |
| BclI | TGATCA | 2 cut(s) | 1696, 1912 |
| BcnI | CCSGG | 2 cut(s) | 1691, 1728 |
| BcoDI | GTCTC | 8 cut(s) | 30, 596, 598, 610, 631, 803, 944, 1208 |
| BfaI | CTAG | 7 cut(s) | 6, 887, 1362, 1407, 1508, 2018, 3054 |
| BfmI | CTRYAG | 3 cut(s) | 630, 1753, 1810 |
| BfoI | RGCGCY | 1 cut(s) | 440 |
| BfuAI | ACCTGC | 1 cut(s) | 401 |
| BfuI | GTATCC | 2 cut(s) | 936, 1368 |
| BglII | AGATCT | 2 cut(s) | 2014, 2305 |
| Bme1390I | CCNGG | 6 cut(s) | 1354, 1412, 1691, 1728, 1862, 2220 |
| Bme18I | GGWCC | 5 cut(s) | 290, 647, 814, 1687, 1958 |
| BmiI | GGNNCC | 8 cut(s) | 316, 816, 1525, 1724, 1775, 2037, 2400, 3037 |
| BmrFI | CCNGG | 6 cut(s) | 1354, 1412, 1691, 1728, 1862, 2220 |
| BmrI | ACTGGG | 3 cut(s) | 321, 2033, 3057 |
| BmsI | GCATC | 9 cut(s) | 136, 264, 721, 730, 759, 1309, 1331, 2750, 2756 |
| BmuI | ACTGGG | 3 cut(s) | 321, 2033, 3057 |
| BpiI | GAAGAC | 1 cut(s) | 1388 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 788, 820 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1457 |
| BpuEI | CTTGAG | 3 cut(s) | 1436, 1778, 2966 |
| BpuMI | CCSGG | 2 cut(s) | 1691, 1728 |
| Bsa29I | ATCGAT | 1 cut(s) | 2676 |
| BsaBI | GATNNNNATC | 1 cut(s) | 2013 |
| BsaI | GGTCTC | 2 cut(s) | 596, 598 |
| BsaJI | CCNNGG | 5 cut(s) | 490, 597, 1726, 2219, 2883 |
| BsaWI | WCCGGW | 1 cut(s) | 973 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 2516, 2546 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 307, 403, 491, 1023, 1367, 1545 |
| Bse1I | ACTGG | 6 cut(s) | 327, 848, 1592, 2039, 2784, 3052 |
| Bse21I | CCTNAGG | 2 cut(s) | 1101, 2791 |
| Bse3DI | GCAATG | 5 cut(s) | 1434, 1882, 2632, 2956, 2975 |
| Bse8I | GATNNNNATC | 1 cut(s) | 2013 |
| BseBI | CCWGG | 4 cut(s) | 1354, 1412, 1862, 2220 |
| BseCI | ATCGAT | 1 cut(s) | 2676 |
| BseDI | CCNNGG | 5 cut(s) | 490, 597, 1726, 2219, 2883 |
| BseJI | GATNNNNATC | 1 cut(s) | 2013 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 307, 403, 491, 1023, 1367, 1545 |
| BseMI | GCAATG | 5 cut(s) | 1434, 1882, 2632, 2956, 2975 |
| BseMII | CTCAG | 6 cut(s) | 540, 798, 1115, 1554, 2169, 2805 |
| BseNI | ACTGG | 6 cut(s) | 327, 848, 1592, 2039, 2784, 3052 |
| BsePI | GCGCGC | 1 cut(s) | 523 |
| BseSI | GKGCMC | 1 cut(s) | 2039 |
| BseXI | GCAGC | 7 cut(s) | 52, 837, 859, 1436, 1986, 2009, 3006 |
| BseYI | CCCAGC | 1 cut(s) | 1519 |
| BsgI | GTGCAG | 1 cut(s) | 1443 |
| Bsh1236I | CGCG | 3 cut(s) | 523, 525, 1219 |
| BshFI | GGCC | 6 cut(s) | 495, 968, 1865, 2037, 2281, 3038 |
| BshNI | GGYRCC | 1 cut(s) | 1523 |
| BshVI | ATCGAT | 1 cut(s) | 2676 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1036, 1142 |
| BsiSI | CCGG | 4 cut(s) | 47, 974, 1690, 1728 |
| BslFI | GGGAC | 1 cut(s) | 329 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 307, 403, 491, 1023, 1367, 1545 |
| BsmAI | GTCTC | 8 cut(s) | 30, 596, 598, 610, 631, 803, 944, 1208 |
| BsmBI | CGTCTC | 2 cut(s) | 803, 1208 |
| BsmFI | GGGAC | 1 cut(s) | 329 |
| BsmI | GAATGC | 4 cut(s) | 831, 1534, 1562, 2892 |
| BsnI | GGCC | 6 cut(s) | 495, 968, 1865, 2037, 2281, 3038 |
| Bso31I | GGTCTC | 2 cut(s) | 596, 598 |
| Bsp120I | GGGCCC | 1 cut(s) | 2035 |
| Bsp1286I | GDGCHC | 3 cut(s) | 1036, 1142, 2039 |
| Bsp1407I | TGTACA | 3 cut(s) | 34, 1111, 1939 |
| Bsp19I | CCATGG | 1 cut(s) | 490 |
| BspANI | GGCC | 6 cut(s) | 495, 968, 1865, 2037, 2281, 3038 |
| BspCNI | CTCAG | 6 cut(s) | 541, 799, 1114, 1555, 2170, 2804 |
| BspDI | ATCGAT | 1 cut(s) | 2676 |
| BspFNI | CGCG | 3 cut(s) | 523, 525, 1219 |
| BspHI | TCATGA | 1 cut(s) | 1894 |
| BspLI | GGNNCC | 8 cut(s) | 316, 816, 1525, 1724, 1775, 2037, 2400, 3037 |
| BspMAI | CTGCAG | 2 cut(s) | 1757, 1814 |
| BspMI | ACCTGC | 1 cut(s) | 401 |
| BspPI | GGATC | 8 cut(s) | 561, 710, 1091, 1998, 2318, 2393, 2406, 2700 |
| BspQI | GCTCTTC | 1 cut(s) | 3009 |
| BspT107I | GGYRCC | 1 cut(s) | 1523 |
| BspTNI | GGTCTC | 2 cut(s) | 596, 598 |
| BsrBI | CCGCTC | 1 cut(s) | 1608 |
| BsrDI | GCAATG | 5 cut(s) | 1434, 1882, 2632, 2956, 2975 |
| BsrGI | TGTACA | 3 cut(s) | 34, 1111, 1939 |
| BsrI | ACTGG | 6 cut(s) | 327, 848, 1592, 2039, 2784, 3052 |
| BssECI | CCNNGG | 5 cut(s) | 490, 597, 1726, 2219, 2883 |
| BssHII | GCGCGC | 1 cut(s) | 523 |
| BssT1I | CCWWGG | 2 cut(s) | 490, 2883 |
| Bst2UI | CCWGG | 4 cut(s) | 1354, 1412, 1862, 2220 |
| Bst4CI | ACNGT | 6 cut(s) | 429, 938, 2443, 2518, 2692, 2722 |
| Bst6I | CTCTTC | 2 cut(s) | 33, 3009 |
| BstAUI | TGTACA | 3 cut(s) | 34, 1111, 1939 |
| BstC8I | GCNNGC | 4 cut(s) | 222, 525, 1081, 1221 |
| BstDEI | CTNAG | 8 cut(s) | 549, 807, 1101, 1457, 1563, 2178, 2334, 2791 |
| BstDSI | CCRYGG | 2 cut(s) | 490, 597 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1365 |
| BstFNI | CGCG | 3 cut(s) | 523, 525, 1219 |
| BstH2I | RGCGCY | 1 cut(s) | 440 |
| BstHHI | GCGC | 3 cut(s) | 439, 525, 527 |
| BstMAI | GTCTC | 8 cut(s) | 30, 596, 598, 610, 631, 803, 944, 1208 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 217, 533, 577, 740, 879, 1876, 1980, 2003 |
| BstNI | CCWGG | 4 cut(s) | 1354, 1412, 1862, 2220 |
| BstNSI | RCATGY | 7 cut(s) | 467, 623, 760, 1422, 1674, 2093, 2130 |
| BstSCI | CCNGG | 6 cut(s) | 1352, 1410, 1689, 1726, 1860, 2218 |
| BstSFI | CTRYAG | 3 cut(s) | 630, 1753, 1810 |
| BstSLI | GKGCMC | 1 cut(s) | 2039 |
| BstUI | CGCG | 3 cut(s) | 523, 525, 1219 |
| BstV1I | GCAGC | 7 cut(s) | 52, 837, 859, 1436, 1986, 2009, 3006 |
| BstV2I | GAAGAC | 1 cut(s) | 1388 |
| BstX2I | RGATCY | 5 cut(s) | 553, 2014, 2305, 2398, 2705 |
| BstYI | RGATCY | 5 cut(s) | 553, 2014, 2305, 2398, 2705 |
| Bsu15I | ATCGAT | 1 cut(s) | 2676 |
| Bsu36I | CCTNAGG | 2 cut(s) | 1101, 2791 |
| BsuI | GTATCC | 2 cut(s) | 936, 1368 |
| BsuRI | GGCC | 6 cut(s) | 495, 968, 1865, 2037, 2281, 3038 |
| BsuTUI | ATCGAT | 1 cut(s) | 2676 |
| BtgI | CCRYGG | 2 cut(s) | 490, 597 |
| BtgZI | GCGATG | 1 cut(s) | 2969 |
| BtsIMutI | CAGTG | 3 cut(s) | 1142, 2433, 3039 |
| BveI | ACCTGC | 1 cut(s) | 401 |
| Cac8I | GCNNGC | 4 cut(s) | 222, 525, 1081, 1221 |
| CaiI | CAGNNNCTG | 1 cut(s) | 2300 |
| CciI | TCATGA | 1 cut(s) | 1894 |
| CfoI | GCGC | 3 cut(s) | 439, 525, 527 |
| ClaI | ATCGAT | 1 cut(s) | 2676 |
| CpoI | CGGWCCG | 1 cut(s) | 1687 |
| CseI | GACGC | 1 cut(s) | 1225 |
| CsiI | ACCWGGT | 1 cut(s) | 1352 |
| CspI | CGGWCCG | 1 cut(s) | 1687 |
| DdeI | CTNAG | 8 cut(s) | 549, 807, 1101, 1457, 1563, 2178, 2334, 2791 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 645 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 645 |
| Eam1104I | CTCTTC | 2 cut(s) | 33, 3009 |
| EarI | CTCTTC | 2 cut(s) | 33, 3009 |
| Ecl136II | GAGCTC | 1 cut(s) | 1034 |
| Eco130I | CCWWGG | 2 cut(s) | 490, 2883 |
| Eco147I | AGGCCT | 1 cut(s) | 1865 |
| Eco24I | GRGCYC | 2 cut(s) | 1036, 2039 |
| Eco31I | GGTCTC | 2 cut(s) | 596, 598 |
| Eco32I | GATATC | 1 cut(s) | 2674 |
| Eco47I | GGWCC | 5 cut(s) | 290, 647, 814, 1687, 1958 |
| Eco47III | AGCGCT | 1 cut(s) | 438 |
| Eco53kI | GAGCTC | 1 cut(s) | 1034 |
| Eco57I | CTGAAG | 3 cut(s) | 519, 2322, 2553 |
| Eco81I | CCTNAGG | 2 cut(s) | 1101, 2791 |
| EcoICRI | GAGCTC | 1 cut(s) | 1034 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1365 |
| EcoO109I | RGGNCCY | 2 cut(s) | 2035, 2280 |
| EcoRI | GAATTC | 1 cut(s) | 1433 |
| EcoRII | CCWGG | 4 cut(s) | 1352, 1410, 1860, 2218 |
| EcoRV | GATATC | 1 cut(s) | 2674 |
| EcoT14I | CCWWGG | 2 cut(s) | 490, 2883 |
| EcoT22I | ATGCAT | 1 cut(s) | 1324 |
| EcoT38I | GRGCYC | 2 cut(s) | 1036, 2039 |
| ErhI | CCWWGG | 2 cut(s) | 490, 2883 |
| Esp3I | CGTCTC | 2 cut(s) | 803, 1208 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 2295, 2327 |
| FaqI | GGGAC | 1 cut(s) | 329 |
| FauI | CCCGC | 4 cut(s) | 1155, 2143, 2323, 2491 |
| FauNDI | CATATG | 2 cut(s) | 721, 1262 |
| FbaI | TGATCA | 2 cut(s) | 1696, 1912 |
| FblI | GTMKAC | 2 cut(s) | 367, 1488 |
| FriOI | GRGCYC | 2 cut(s) | 1036, 2039 |
| FspBI | CTAG | 7 cut(s) | 6, 887, 1362, 1407, 1508, 2018, 3054 |
| GlaI | GCGC | 3 cut(s) | 438, 524, 526 |
| GsaI | CCCAGC | 1 cut(s) | 1523 |
| HaeII | RGCGCY | 1 cut(s) | 440 |
| HaeIII | GGCC | 6 cut(s) | 495, 968, 1865, 2037, 2281, 3038 |
| HapII | CCGG | 4 cut(s) | 47, 974, 1690, 1728 |
| HgaI | GACGC | 1 cut(s) | 1225 |
| HhaI | GCGC | 3 cut(s) | 439, 525, 527 |
| Hin6I | GCGC | 3 cut(s) | 437, 523, 525 |
| HinP1I | GCGC | 3 cut(s) | 437, 523, 525 |
| HincII | GTYRAC | 3 cut(s) | 368, 1268, 2191 |
| HindII | GTYRAC | 3 cut(s) | 368, 1268, 2191 |
| HindIII | AAGCTT | 3 cut(s) | 1153, 1454, 2565 |
| HpaII | CCGG | 4 cut(s) | 47, 974, 1690, 1728 |
| HphI | GGTGA | 3 cut(s) | 173, 418, 1615 |
| Hpy99I | CGWCG | 2 cut(s) | 190, 714 |
| HpyAV | CCTTC | 9 cut(s) | 79, 86, 572, 893, 1230, 1561, 1658, 2420, 2886 |
| HpyCH4III | ACNGT | 6 cut(s) | 429, 938, 2443, 2518, 2692, 2722 |
| HpyCH4IV | ACGT | 2 cut(s) | 684, 712 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 217, 533, 577, 740, 879, 1876, 1980, 2003 |
| HpyF3I | CTNAG | 8 cut(s) | 549, 807, 1101, 1457, 1563, 2178, 2334, 2791 |
| HpySE526I | ACGT | 2 cut(s) | 684, 712 |
| HspAI | GCGC | 3 cut(s) | 437, 523, 525 |
| KpnI | GGTACC | 1 cut(s) | 1527 |
| Ksp22I | TGATCA | 2 cut(s) | 1696, 1912 |
| LguI | GCTCTTC | 1 cut(s) | 3009 |
| LmnI | GCTCC | 4 cut(s) | 320, 1884, 2431, 2629 |
| Lsp1109I | GCAGC | 7 cut(s) | 52, 837, 859, 1436, 1986, 2009, 3006 |
| LweI | GCATC | 9 cut(s) | 136, 264, 721, 730, 759, 1309, 1331, 2750, 2756 |
| MabI | ACCWGGT | 1 cut(s) | 1352 |
| MaeI | CTAG | 7 cut(s) | 6, 887, 1362, 1407, 1508, 2018, 3054 |
| MaeII | ACGT | 2 cut(s) | 684, 712 |
| MaeIII | GTNAC | 4 cut(s) | 101, 1681, 2223, 2361 |
| MbiI | CCGCTC | 1 cut(s) | 1608 |
| MflI | RGATCY | 5 cut(s) | 553, 2014, 2305, 2398, 2705 |
| MhlI | GDGCHC | 3 cut(s) | 1036, 1142, 2039 |
| MlyI | GAGTC | 3 cut(s) | 1000, 1640, 2948 |
| MmeI | TCCRAC | 2 cut(s) | 195, 209 |
| Mph1103I | ATGCAT | 1 cut(s) | 1324 |
| MroXI | GAANNNNTTC | 1 cut(s) | 267 |
| MslI | CAYNNNNRTG | 5 cut(s) | 755, 761, 2454, 2469, 2667 |
| MspA1I | CMGCKG | 1 cut(s) | 850 |
| MspI | CCGG | 4 cut(s) | 47, 974, 1690, 1728 |
| MspR9I | CCNGG | 6 cut(s) | 1354, 1412, 1691, 1728, 1862, 2220 |
| Mva1269I | GAATGC | 4 cut(s) | 831, 1534, 1562, 2892 |
| MvaI | CCWGG | 4 cut(s) | 1354, 1412, 1862, 2220 |
| MvnI | CGCG | 3 cut(s) | 523, 525, 1219 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 217, 533, 577, 740, 879, 1876, 1980, 2003 |
| NciI | CCSGG | 2 cut(s) | 1691, 1728 |
| NcoI | CCATGG | 1 cut(s) | 490 |
| NdeI | CATATG | 2 cut(s) | 721, 1262 |
| NlaIV | GGNNCC | 8 cut(s) | 316, 816, 1525, 1724, 1775, 2037, 2400, 3037 |
| NmuCI | GTSAC | 1 cut(s) | 101 |
| NsiI | ATGCAT | 1 cut(s) | 1324 |
| NspI | RCATGY | 7 cut(s) | 467, 623, 760, 1422, 1674, 2093, 2130 |
| PagI | TCATGA | 1 cut(s) | 1894 |
| PauI | GCGCGC | 1 cut(s) | 523 |
| PceI | AGGCCT | 1 cut(s) | 1865 |
| PciI | ACATGT | 2 cut(s) | 1418, 1670 |
| PciSI | GCTCTTC | 1 cut(s) | 3009 |
| PctI | GAATGC | 4 cut(s) | 831, 1534, 1562, 2892 |
| PdmI | GAANNNNTTC | 1 cut(s) | 267 |
| PflMI | CCANNNNNTGG | 3 cut(s) | 307, 403, 491 |
| PleI | GAGTC | 3 cut(s) | 999, 1640, 2947 |
| PpsI | GAGTC | 3 cut(s) | 999, 1640, 2947 |
| PscI | ACATGT | 2 cut(s) | 1418, 1670 |
| PsiI | TTATAA | 1 cut(s) | 2727 |
| Psp124BI | GAGCTC | 1 cut(s) | 1036 |
| Psp6I | CCWGG | 4 cut(s) | 1352, 1410, 1860, 2218 |
| PspFI | CCCAGC | 1 cut(s) | 1519 |
| PspGI | CCWGG | 4 cut(s) | 1352, 1410, 1860, 2218 |
| PspN4I | GGNNCC | 8 cut(s) | 316, 816, 1525, 1724, 1775, 2037, 2400, 3037 |
| PspOMI | GGGCCC | 1 cut(s) | 2035 |
| PstI | CTGCAG | 2 cut(s) | 1757, 1814 |
| PstNI | CAGNNNCTG | 1 cut(s) | 2300 |
| PsuI | RGATCY | 5 cut(s) | 553, 2014, 2305, 2398, 2705 |
| PteI | GCGCGC | 1 cut(s) | 523 |
| PvuII | CAGCTG | 1 cut(s) | 850 |
| RseI | CAYNNNNRTG | 5 cut(s) | 755, 761, 2454, 2469, 2667 |
| Rsr2I | CGGWCCG | 1 cut(s) | 1687 |
| RsrII | CGGWCCG | 1 cut(s) | 1687 |
| SacI | GAGCTC | 1 cut(s) | 1036 |
| SalI | GTCGAC | 1 cut(s) | 366 |
| SapI | GCTCTTC | 1 cut(s) | 3009 |
| SchI | GAGTC | 3 cut(s) | 1000, 1640, 2948 |
| ScrFI | CCNGG | 6 cut(s) | 1354, 1412, 1691, 1728, 1862, 2220 |
| SduI | GDGCHC | 3 cut(s) | 1036, 1142, 2039 |
| SexAI | ACCWGGT | 1 cut(s) | 1352 |
| SfaNI | GCATC | 9 cut(s) | 136, 264, 721, 730, 759, 1309, 1331, 2750, 2756 |
| SfcI | CTRYAG | 3 cut(s) | 630, 1753, 1810 |
| SinI | GGWCC | 5 cut(s) | 290, 647, 814, 1687, 1958 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 755, 761, 2454, 2469, 2667 |
| SmlI | CTYRAG | 3 cut(s) | 1451, 1793, 2981 |
| SmoI | CTYRAG | 3 cut(s) | 1451, 1793, 2981 |
| SseBI | AGGCCT | 1 cut(s) | 1865 |
| SspMI | CTAG | 7 cut(s) | 6, 887, 1362, 1407, 1508, 2018, 3054 |
| SstI | GAGCTC | 1 cut(s) | 1036 |
| StuI | AGGCCT | 1 cut(s) | 1865 |
| StyD4I | CCNGG | 6 cut(s) | 1352, 1410, 1689, 1726, 1860, 2218 |
| StyI | CCWWGG | 2 cut(s) | 490, 2883 |
| TaaI | ACNGT | 6 cut(s) | 429, 938, 2443, 2518, 2692, 2722 |
| TaiI | ACGT | 2 cut(s) | 687, 715 |
| TaqI | TCGA | 4 cut(s) | 83, 367, 709, 2676 |
| TatI | WGTACW | 4 cut(s) | 34, 1111, 1939, 2776 |
| TauI | GCSGC | 4 cut(s) | 214, 217, 220, 315 |
| TscAI | CASTG | 3 cut(s) | 1142, 2433, 3046 |
| TseFI | GTSAC | 1 cut(s) | 101 |
| TseI | GCWGC | 7 cut(s) | 65, 847, 850, 1424, 1974, 1997, 3019 |
| Tsp45I | GTSAC | 1 cut(s) | 101 |
| TspGWI | ACGGA | 6 cut(s) | 586, 827, 1700, 2003, 2411, 2628 |
| TspRI | CASTG | 3 cut(s) | 1142, 2433, 3046 |
| Van91I | CCANNNNNTGG | 3 cut(s) | 307, 403, 491 |
| VpaK11BI | GGWCC | 5 cut(s) | 290, 647, 814, 1687, 1958 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1365 |
| XapI | RAATTY | 3 cut(s) | 358, 998, 1433 |
| XbaI | TCTAGA | 1 cut(s) | 2017 |
| XceI | RCATGY | 7 cut(s) | 467, 623, 760, 1422, 1674, 2093, 2130 |
| XmiI | GTMKAC | 2 cut(s) | 367, 1488 |
| XmnI | GAANNNNTTC | 1 cut(s) | 267 |
| XspI | CTAG | 7 cut(s) | 6, 887, 1362, 1407, 1508, 2018, 3054 |
| Zsp2I | ATGCAT | 1 cut(s) | 1324 |