Rh5BG562400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000990
Physical Location & Seq
Reverse (-)
89160190 .. 89161977
1788 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG562400.1

Sequence Viewer

Length: 1788 bp
ATGGAATTCTACCACAACCAACTCAAGCTTAGGTTATTGAATGAGAAGAAACCCAGTGTCTACAAAAGAGTTGATTGGCTGGTTGATAAGCTGGGTACCCAAGTGCATTCCTATTTCTGGCTTGATGAATACTCTGAGAAGGATGATTTTGCACGATACTGGAAAGATGAGTGGGTGAACGGTTTAACAGCTTGGCGAACAGCTTTGGAGATTCCAGACTCTAATGTTGTCATTGAAGGTACACGTGCAAAAGTAACAGACCAGCTTGATCAAGAAAAGGCTTATGTTTTTTGGAACCCTGGTTCACAATTCAATATTTGCAACTGCAGTTGGGCAGAAATGGGGAACCTGTGTGAGCATATACTCAAGGTTATTAGTGTCTGCAGGAAAAGAAGTCATCCTGTGCCATCTGTCAGCTTATTGCAGTTCCACCAGGCCTTGCTTGATATGCTCCATTGCCCACCTCATGATTCTTTAATTCGTGATCATGCTGTTTCTTTAGCGGTCTTTGTACAGAACCAGTTAAGTGGACCTAATTTGGAAAGCAGCAATAGCACAATGGATCAGGCAGCTTTAGCCGATAAAGATCTAGAAATAGTAAATGAGGGCCCAGTAAGTGAAGAAGTATTATCTCATAATGAGAATAGTTGTGCGGATGAGCATGTTGCTGTGGGTGCTGAAACGAGTAGTCTAGTTGCATGTGGAAATGGCATATATAATGAGGGTTGTGGAGAAGAAATCACTTCTTCTGAGATGGATGTTGATTCATCATCCATCTGTATTTCTCCACCTGGGTTACATTCTGTTGATGAGGTTGTGTCTAGTGGTGTCCTCTCAGGAAGCAGACAGAGGTCCTTGTTTAACAGAGAAACTGAAGATCTGCTTTCCACAGATGATCCTCTCACTAATCCAAGTGAATATGTGGATGATATTTTAGATGAAAATTGCGAAAAAAATGTGATGAGTACGGATCCACTCTCCTTTCATATCCCTTGTTTAACAATGGAGTTTGTGGAGCAATGCACGGTACCCCATCCTAATGATCTCCACAGCCATGAAGTCAATCCCACTGTTATTTCGAAAGTATGTGATGACAATACTGTTAATAACAATACCTCCCCATCTTCGTCTGTGCCTCATGAACCACAAGCTTCAGGTGTCATAGAAAAAGAGAACATGGAAATTGAAAGTATGGACGGAATGGACTTTGTGGAGCATTGCGCATTAACCAATCCCAACAGTAACGACAGCCATGATATCGATATTGAGCCTACTGTTATTGCCAAAAGATCTCCGGACAATACTGTTTTTAACAAGACCTCTGCATCTTCATTTATGCCTGTTGAATCAGAAATGGTTGAAGTGGCTGAAACTTCAGGTTTCATTTTAGAAAATGATAGAATGGAAATTGGAAGTGAGAATGGTAGCACAAGCAAGCACAATCTGTCTGCTAATAATCCTTCCACCTTGGACGGCATTCGTGGTTGTATGATAAATGGTTCAGATTGTTGTCGGGATTCTGAGTCAGTTGGCAATGCGATGGTTGTGTACTCAGAACCATTGCTCAAGTTCTCATCAACATCAAGTTGCAACAAAGAAGAGCTGCCTGTTGAGAATGGGGCTACTGAAACTGGGTCTGGTGAGAAGCCATCTTCAATCACTGAATCTTATCTTAAAAAGTGTAAAGTTCAAGAGGGTGCCGAAATCATCAACAACAATGAGGTGATCCACCAGCCAGTTATCAGTGACATCAATGTTTACTTCTGTAAAGGGAAATGCTCGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

595

Amino Acids

65.72

Weight (kDa)

4.55

Isoelectric Point (pI)

43.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1222
Acc65I GGTACC 2 cut(s) 95, 1027
AccB1I GGYRCC 3 cut(s) 95, 1027, 1697
AccI GTMKAC 1 cut(s) 60
AccIII TCCGGA 1 cut(s) 1294
AciI CCGC 2 cut(s) 503, 653
AclWI GGATC 5 cut(s) 570, 890, 965, 978, 1720
AcsI RAATTY 1 cut(s) 5
AcuI CTGAAG 3 cut(s) 894, 1137, 1359
AcvI CACGTG 1 cut(s) 245
AfaI GTAC 6 cut(s) 97, 241, 513, 967, 1029, 1550
AfiI CCNNNNNNNGG 1 cut(s) 117
AflIII ACRYGT 1 cut(s) 242
AgsI TTSAA 8 cut(s) 40, 236, 313, 1187, 1346, 1361, 1656, 1691
AjnI CCWGG 3 cut(s) 298, 432, 790
AloI GAACNNNNNNTCC 2 cut(s) 286, 318
AluBI AGCT 9 cut(s) 28, 91, 191, 203, 265, 417, 572, 1151, 1603
AluI AGCT 9 cut(s) 28, 91, 191, 203, 265, 417, 572, 1151, 1603
AlwI GGATC 5 cut(s) 570, 890, 965, 978, 1720
Ama87I CYCGRG 1 cut(s) 1780
Aor13HI TCCGGA 1 cut(s) 1294
AoxI GGCC 2 cut(s) 435, 607
ApaI GGGCCC 1 cut(s) 611
ApeKI GCWGC 3 cut(s) 546, 569, 1603
ApoI RAATTY 1 cut(s) 5
Asp718I GGTACC 2 cut(s) 95, 1027
AspLEI GCGC 1 cut(s) 1223
AspS9I GGNCC 4 cut(s) 530, 607, 608, 852
AsuHPI GGTGA 3 cut(s) 187, 1652, 1735
AsuII TTCGAA 1 cut(s) 1079
AvaI CYCGRG 1 cut(s) 1780
AvaII GGWCC 2 cut(s) 530, 852
BaeGI GKGCMC 1 cut(s) 611
BaeI ACNNNNGTAYC 2 cut(s) 87, 120
BamHI GGATCC 1 cut(s) 970
BanI GGYRCC 3 cut(s) 95, 1027, 1697
BanII GRGCYC 1 cut(s) 611
BbrPI CACGTG 1 cut(s) 245
BbvI GCAGC 3 cut(s) 558, 581, 1590
BccI CCATC 7 cut(s) 415, 748, 782, 1041, 1129, 1534, 1657
BceAI ACGGC 1 cut(s) 1489
BciT130I CCWGG 3 cut(s) 300, 434, 792
BclI TGATCA 2 cut(s) 268, 484
BfaI CTAG 3 cut(s) 590, 692, 822
BfmI CTRYAG 2 cut(s) 325, 382
BglII AGATCT 3 cut(s) 586, 877, 1289
BisI GCNGC 3 cut(s) 547, 570, 1604
BlsI GCNGC 3 cut(s) 548, 571, 1605
Bme1390I CCNGG 3 cut(s) 300, 434, 792
Bme18I GGWCC 2 cut(s) 530, 852
BmeT110I CYCGRG 1 cut(s) 1780
BmgT120I GGNCC 4 cut(s) 530, 607, 608, 852
BmiI GGNNCC 7 cut(s) 97, 296, 347, 609, 972, 1029, 1699
BmrFI CCNGG 3 cut(s) 300, 434, 792
BmrI ACTGGG 3 cut(s) 48, 605, 1641
BmsI GCATC 1 cut(s) 1334
BmuI ACTGGG 3 cut(s) 48, 605, 1641
BoxI GACNNNNGTC 1 cut(s) 850
Bpu10I CCTNAGC 1 cut(s) 29
Bpu14I TTCGAA 1 cut(s) 1079
BpuEI CTTGAG 3 cut(s) 8, 350, 1550
Bsa29I ATCGAT 1 cut(s) 1260
BsaAI YACGTR 1 cut(s) 245
BsaBI GATNNNNATC 1 cut(s) 585
BsaJI CCNNGG 3 cut(s) 298, 791, 1467
BsaWI WCCGGW 1 cut(s) 1294
BsaXI ACNNNNNCTCC 2 cut(s) 1100, 1130
Bsc4I CCNNNNNNNGG 1 cut(s) 117
Bse1I ACTGG 6 cut(s) 54, 164, 520, 611, 1636, 1736
Bse3DI GCAATG 5 cut(s) 454, 1025, 1216, 1540, 1559
Bse8I GATNNNNATC 1 cut(s) 585
BseAI TCCGGA 1 cut(s) 1294
BseBI CCWGG 3 cut(s) 300, 434, 792
BseCI ATCGAT 1 cut(s) 1260
BseDI CCNNGG 3 cut(s) 298, 791, 1467
BseGI GGATG 7 cut(s) 148, 397, 661, 763, 770, 931, 1033
BseJI GATNNNNATC 1 cut(s) 585
BseLI CCNNNNNNNGG 1 cut(s) 117
BseMI GCAATG 5 cut(s) 454, 1025, 1216, 1540, 1559
BseMII CTCAG 5 cut(s) 126, 741, 849, 1512, 1566
BseNI ACTGG 6 cut(s) 54, 164, 520, 611, 1636, 1736
BseSI GKGCMC 1 cut(s) 611
BseXI GCAGC 3 cut(s) 558, 581, 1590
BseYI CCCAGC 1 cut(s) 91
BshFI GGCC 2 cut(s) 437, 609
BshNI GGYRCC 3 cut(s) 95, 1027, 1697
BshVI ATCGAT 1 cut(s) 1260
BsiHKCI CYCGRG 1 cut(s) 1780
BsiSI CCGG 1 cut(s) 1295
BslI CCNNNNNNNGG 1 cut(s) 117
BsmI GAATGC 2 cut(s) 106, 1476
BsnI GGCC 2 cut(s) 437, 609
BsoBI CYCGRG 1 cut(s) 1780
Bsp119I TTCGAA 1 cut(s) 1079
Bsp120I GGGCCC 1 cut(s) 607
Bsp1286I GDGCHC 1 cut(s) 611
Bsp13I TCCGGA 1 cut(s) 1294
Bsp1407I TGTACA 1 cut(s) 511
BspACI CCGC 2 cut(s) 503, 653
BspANI GGCC 2 cut(s) 437, 609
BspCNI CTCAG 5 cut(s) 127, 742, 848, 1513, 1565
BspDI ATCGAT 1 cut(s) 1260
BspEI TCCGGA 1 cut(s) 1294
BspHI TCATGA 2 cut(s) 466, 1138
BspLI GGNNCC 7 cut(s) 97, 296, 347, 609, 972, 1029, 1699
BspMAI CTGCAG 2 cut(s) 329, 386
BspPI GGATC 5 cut(s) 570, 890, 965, 978, 1720
BspQI GCTCTTC 1 cut(s) 1593
BspT104I TTCGAA 1 cut(s) 1079
BspT107I GGYRCC 3 cut(s) 95, 1027, 1697
BsrDI GCAATG 5 cut(s) 454, 1025, 1216, 1540, 1559
BsrGI TGTACA 1 cut(s) 511
BsrI ACTGG 6 cut(s) 54, 164, 520, 611, 1636, 1736
BssECI CCNNGG 3 cut(s) 298, 791, 1467
BssT1I CCWWGG 1 cut(s) 1467
Bst2UI CCWGG 3 cut(s) 300, 434, 792
Bst4CI ACNGT 7 cut(s) 182, 1027, 1072, 1102, 1241, 1276, 1306
Bst6I CTCTTC 1 cut(s) 1593
BstAUI TGTACA 1 cut(s) 511
BstBAI YACGTR 1 cut(s) 245
BstBI TTCGAA 1 cut(s) 1079
BstC8I GCNNGC 1 cut(s) 1436
BstDEI CTNAG 6 cut(s) 29, 135, 750, 835, 1521, 1552
BstF5I GGATG 7 cut(s) 148, 397, 661, 763, 770, 931, 1033
BstHHI GCGC 1 cut(s) 1223
BstMWI GCNNNNNNNGC 4 cut(s) 448, 552, 575, 674
BstNI CCWGG 3 cut(s) 300, 434, 792
BstNSI RCATGY 2 cut(s) 665, 702
BstPAI GACNNNNGTC 1 cut(s) 850
BstSCI CCNGG 3 cut(s) 298, 432, 790
BstSFI CTRYAG 2 cut(s) 325, 382
BstSLI GKGCMC 1 cut(s) 611
BstV1I GCAGC 3 cut(s) 558, 581, 1590
BstX2I RGATCY 4 cut(s) 586, 877, 970, 1289
BstXI CCANNNNNNTGG 1 cut(s) 527
BstYI RGATCY 4 cut(s) 586, 877, 970, 1289
Bsu15I ATCGAT 1 cut(s) 1260
BsuRI GGCC 2 cut(s) 437, 609
BsuTUI ATCGAT 1 cut(s) 1260
BtgZI GCGATG 1 cut(s) 1553
BtsCI GGATG 7 cut(s) 148, 397, 661, 763, 770, 931, 1033
BtsIMutI CAGTG 4 cut(s) 61, 1068, 1659, 1750
Cac8I GCNNGC 1 cut(s) 1436
CciI TCATGA 2 cut(s) 466, 1138
CfoI GCGC 1 cut(s) 1223
Cfr13I GGNCC 4 cut(s) 530, 607, 608, 852
ClaI ATCGAT 1 cut(s) 1260
Csp6I GTAC 6 cut(s) 96, 240, 512, 966, 1028, 1549
CviAII CATG 8 cut(s) 467, 488, 662, 699, 1055, 1139, 1177, 1253
CviQI GTAC 6 cut(s) 96, 240, 512, 966, 1028, 1549
DdeI CTNAG 6 cut(s) 29, 135, 750, 835, 1521, 1552
Eam1104I CTCTTC 1 cut(s) 1593
EarI CTCTTC 1 cut(s) 1593
Eco130I CCWWGG 1 cut(s) 1467
Eco147I AGGCCT 1 cut(s) 437
Eco24I GRGCYC 1 cut(s) 611
Eco32I GATATC 1 cut(s) 1258
Eco47I GGWCC 2 cut(s) 530, 852
Eco57I CTGAAG 3 cut(s) 894, 1137, 1359
Eco72I CACGTG 1 cut(s) 245
Eco88I CYCGRG 1 cut(s) 1780
EcoO109I RGGNCCY 2 cut(s) 607, 852
EcoRI GAATTC 1 cut(s) 5
EcoRII CCWGG 3 cut(s) 298, 432, 790
EcoRV GATATC 1 cut(s) 1258
EcoT14I CCWWGG 1 cut(s) 1467
EcoT38I GRGCYC 1 cut(s) 611
ErhI CCWWGG 1 cut(s) 1467
FaeI CATG 8 cut(s) 470, 491, 665, 702, 1058, 1142, 1180, 1256
FalI AAGNNNNNCTT 2 cut(s) 867, 899
FatI CATG 8 cut(s) 466, 487, 661, 698, 1054, 1138, 1176, 1252
FbaI TGATCA 2 cut(s) 268, 484
FblI GTMKAC 1 cut(s) 60
Fnu4HI GCNGC 3 cut(s) 547, 570, 1604
FokI GGATG 7 cut(s) 155, 384, 668, 757, 770, 938, 1020
FriOI GRGCYC 1 cut(s) 611
Fsp4HI GCNGC 3 cut(s) 547, 570, 1604
FspBI CTAG 3 cut(s) 590, 692, 822
FspI TGCGCA 1 cut(s) 1222
GlaI GCGC 1 cut(s) 1222
GluI GCNGC 3 cut(s) 547, 570, 1604
GsaI CCCAGC 1 cut(s) 95
HaeIII GGCC 2 cut(s) 437, 609
HapII CCGG 1 cut(s) 1295
HhaI GCGC 1 cut(s) 1223
Hin1II CATG 8 cut(s) 470, 491, 665, 702, 1058, 1142, 1180, 1256
Hin6I GCGC 1 cut(s) 1221
HinP1I GCGC 1 cut(s) 1221
HindIII AAGCTT 2 cut(s) 26, 1149
HinfI GANTC 8 cut(s) 211, 218, 470, 764, 1346, 1517, 1523, 1664
HpaII CCGG 1 cut(s) 1295
HphI GGTGA 3 cut(s) 187, 1652, 1735
Hpy166II GTNNAC 7 cut(s) 61, 178, 242, 305, 530, 1549, 1759
Hpy188I TCNGA 6 cut(s) 136, 751, 1351, 1504, 1522, 1555
Hpy8I GTNNAC 7 cut(s) 61, 178, 242, 305, 530, 1549, 1759
HpyAV CCTTC 3 cut(s) 133, 230, 1470
HpyCH4III ACNGT 7 cut(s) 182, 1027, 1072, 1102, 1241, 1276, 1306
HpyCH4IV ACGT 1 cut(s) 244
HpyF10VI GCNNNNNNNGC 4 cut(s) 448, 552, 575, 674
HpyF3I CTNAG 6 cut(s) 29, 135, 750, 835, 1521, 1552
HpySE526I ACGT 1 cut(s) 244
Hsp92II CATG 8 cut(s) 470, 491, 665, 702, 1058, 1142, 1180, 1256
HspAI GCGC 1 cut(s) 1221
Kpn2I TCCGGA 1 cut(s) 1294
KpnI GGTACC 2 cut(s) 99, 1031
Ksp22I TGATCA 2 cut(s) 268, 484
LguI GCTCTTC 1 cut(s) 1593
LmnI GCTCC 3 cut(s) 456, 1015, 1213
Lsp1109I GCAGC 3 cut(s) 558, 581, 1590
LweI GCATC 1 cut(s) 1334
MaeI CTAG 3 cut(s) 590, 692, 822
MaeII ACGT 1 cut(s) 244
MaeIII GTNAC 4 cut(s) 253, 795, 1241, 1745
MboII GAAGA 9 cut(s) 58, 632, 738, 746, 887, 1116, 1320, 1610, 1644
MflI RGATCY 4 cut(s) 586, 877, 970, 1289
MhlI GDGCHC 1 cut(s) 611
MluCI AATT 7 cut(s) 5, 308, 477, 535, 943, 1182, 1407
MlyI GAGTC 2 cut(s) 212, 1532
MroI TCCGGA 1 cut(s) 1294
MseI TTAA 9 cut(s) 185, 476, 524, 861, 998, 1104, 1226, 1311, 1674
MslI CAYNNNNRTG 2 cut(s) 1038, 1053
MspI CCGG 1 cut(s) 1295
MspR9I CCNGG 3 cut(s) 300, 434, 792
Mva1269I GAATGC 2 cut(s) 106, 1476
MvaI CCWGG 3 cut(s) 300, 434, 792
MwoI GCNNNNNNNGC 4 cut(s) 448, 552, 575, 674
NlaIII CATG 8 cut(s) 470, 491, 665, 702, 1058, 1142, 1180, 1256
NlaIV GGNNCC 7 cut(s) 97, 296, 347, 609, 972, 1029, 1699
NmuCI GTSAC 1 cut(s) 1745
NsbI TGCGCA 1 cut(s) 1222
NspI RCATGY 2 cut(s) 665, 702
NspV TTCGAA 1 cut(s) 1079
PaeR7I CTCGAG 1 cut(s) 1780
PagI TCATGA 2 cut(s) 466, 1138
PceI AGGCCT 1 cut(s) 437
PciSI GCTCTTC 1 cut(s) 1593
PctI GAATGC 2 cut(s) 106, 1476
PfeI GAWTC 6 cut(s) 211, 470, 764, 1346, 1517, 1664
PkrI GCNGC 3 cut(s) 548, 571, 1605
PleI GAGTC 2 cut(s) 212, 1531
PmaCI CACGTG 1 cut(s) 245
PmlI CACGTG 1 cut(s) 245
PpsI GAGTC 2 cut(s) 212, 1531
Ppu21I YACGTR 1 cut(s) 245
PpuMI RGGWCCY 1 cut(s) 852
PshAI GACNNNNGTC 1 cut(s) 850
Psp5II RGGWCCY 1 cut(s) 852
Psp6I CCWGG 3 cut(s) 298, 432, 790
PspCI CACGTG 1 cut(s) 245
PspFI CCCAGC 1 cut(s) 91
PspGI CCWGG 3 cut(s) 298, 432, 790
PspN4I GGNNCC 7 cut(s) 97, 296, 347, 609, 972, 1029, 1699
PspOMI GGGCCC 1 cut(s) 607
PspPI GGNCC 4 cut(s) 530, 607, 608, 852
PspPPI RGGWCCY 1 cut(s) 852
PspXI VCTCGAGB 1 cut(s) 1780
PstI CTGCAG 2 cut(s) 329, 386
PsuI RGATCY 4 cut(s) 586, 877, 970, 1289
RsaI GTAC 6 cut(s) 97, 241, 513, 967, 1029, 1550
RsaNI GTAC 6 cut(s) 96, 240, 512, 966, 1028, 1549
RseI CAYNNNNRTG 2 cut(s) 1038, 1053
SapI GCTCTTC 1 cut(s) 1593
SaqAI TTAA 9 cut(s) 185, 476, 524, 861, 998, 1104, 1226, 1311, 1674
SatI GCNGC 3 cut(s) 547, 570, 1604
Sau96I GGNCC 4 cut(s) 530, 607, 608, 852
SchI GAGTC 2 cut(s) 212, 1532
ScrFI CCNGG 3 cut(s) 300, 434, 792
SduI GDGCHC 1 cut(s) 611
SfaNI GCATC 1 cut(s) 1334
SfcI CTRYAG 2 cut(s) 325, 382
Sfr274I CTCGAG 1 cut(s) 1780
SfuI TTCGAA 1 cut(s) 1079
SinI GGWCC 2 cut(s) 530, 852
SlaI CTCGAG 1 cut(s) 1780
SmiMI CAYNNNNRTG 2 cut(s) 1038, 1053
SmlI CTYRAG 4 cut(s) 23, 365, 1565, 1780
SmoI CTYRAG 4 cut(s) 23, 365, 1565, 1780
Sse9I AATT 7 cut(s) 5, 308, 477, 535, 943, 1182, 1407
SseBI AGGCCT 1 cut(s) 437
SsiI CCGC 2 cut(s) 503, 653
SspI AATATT 1 cut(s) 316
SspMI CTAG 3 cut(s) 590, 692, 822
StuI AGGCCT 1 cut(s) 437
StyD4I CCNGG 3 cut(s) 298, 432, 790
StyI CCWWGG 1 cut(s) 1467
TaaI ACNGT 7 cut(s) 182, 1027, 1072, 1102, 1241, 1276, 1306
TaiI ACGT 1 cut(s) 247
TaqI TCGA 3 cut(s) 1079, 1260, 1781
TasI AATT 7 cut(s) 5, 308, 477, 535, 943, 1182, 1407
TatI WGTACW 2 cut(s) 511, 1548
TfiI GAWTC 6 cut(s) 211, 470, 764, 1346, 1517, 1664
Tru1I TTAA 9 cut(s) 185, 476, 524, 861, 998, 1104, 1226, 1311, 1674
Tru9I TTAA 9 cut(s) 185, 476, 524, 861, 998, 1104, 1226, 1311, 1674
TscAI CASTG 4 cut(s) 61, 1075, 1666, 1750
TseFI GTSAC 1 cut(s) 1745
TseI GCWGC 3 cut(s) 546, 569, 1603
Tsp45I GTSAC 1 cut(s) 1745
TspDTI ATGAA 8 cut(s) 141, 756, 954, 974, 1071, 1155, 1320, 1372
TspGWI ACGGA 2 cut(s) 983, 1212
TspRI CASTG 4 cut(s) 61, 1075, 1666, 1750
VpaK11BI GGWCC 2 cut(s) 530, 852
XapI RAATTY 1 cut(s) 5
XbaI TCTAGA 1 cut(s) 589
XceI RCATGY 2 cut(s) 665, 702
XhoI CTCGAG 1 cut(s) 1780
XmiI GTMKAC 1 cut(s) 60
XspI CTAG 3 cut(s) 590, 692, 822
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.