Rw5G050540

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000990
Physical Location & Seq
Forward (+)
87296587 .. 87297249
663 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G050540.1

Sequence Viewer

Length: 663 bp
ATGGATGTTGACCCATCATCCATCTGTATTTCTCCACCTGGGTTACATTCTGTTGATGAAGTTGTGTCTAGTCATGTCCTCTCACGAAGCAGACAGAGGCCCTTGTTTAGCACAGAAACTGAAGATTTGCTTTCTGCAGATGATCCTATCGCTAATTCAAGTGAATATGAGGATGATATGTTAGATGAAAATTGTGAAAAAAATGTGATGAGTACGGATCCACTCTCCCTTCACATCCCTTGTTCAACAATGGAGTTTGTGGAGCAATGCACAGTACGCCATTCTAATGATCTCCACAGCCATGAAGTCAATCCCGCCGTTGTTTCAAAAGTACATAATGACATTACAGTATATAATAATACCTCCCCATCTTCATCTGTGCCCGTTGAACCACAAGCTTCAGGTGTCATAGAAAATGAGAGCATGGAAATTGAAAGTGTGGACGGAGTGGACTTTGTGGAGCATTGCGCATCAACCAATCCTAATGGTAACCACAGCCATGATATCGATATTGCACCTACTGTTATTTCCAAAAGATCCCCTGACAATACTGTTTATAACAAGACCTCTGCATCTGCATCTATGCCTGTTGAATCAGAAATGGTTGTAGTGGCCGAAACTTCAGGTATCATTTTAGAAAATGATAGAATGGAAATGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

220

Amino Acids

24.01

Weight (kDa)

4.33

Isoelectric Point (pI)

55.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 558
Acc16I TGCGCA 1 cut(s) 469
AciI CCGC 1 cut(s) 315
AclWI GGATC 4 cut(s) 137, 212, 225, 531
AcoI YGGCCR 1 cut(s) 612
AcuI CTGAAG 3 cut(s) 141, 384, 606
AfaI GTAC 3 cut(s) 214, 276, 333
AgsI TTSAA 6 cut(s) 159, 246, 327, 389, 434, 593
AjnI CCWGG 1 cut(s) 37
AluBI AGCT 1 cut(s) 398
AluI AGCT 1 cut(s) 398
AlwI GGATC 4 cut(s) 137, 212, 225, 531
AlwNI CAGNNNCTG 1 cut(s) 119
AoxI GGCC 2 cut(s) 98, 612
AspLEI GCGC 1 cut(s) 470
AspS9I GGNCC 1 cut(s) 99
BaeGI GKGCMC 1 cut(s) 384
BamHI GGATCC 1 cut(s) 217
BccI CCATC 3 cut(s) 22, 29, 376
BceAI ACGGC 1 cut(s) 302
BciT130I CCWGG 1 cut(s) 39
BfaI CTAG 1 cut(s) 69
BfmI CTRYAG 1 cut(s) 135
Bme1390I CCNGG 1 cut(s) 39
BmgT120I GGNCC 1 cut(s) 99
BmiI GGNNCC 1 cut(s) 219
BmrFI CCNGG 1 cut(s) 39
BmsI GCATC 3 cut(s) 479, 581, 587
Bsa29I ATCGAT 1 cut(s) 507
BsaJI CCNNGG 1 cut(s) 38
Bse3DI GCAATG 2 cut(s) 272, 463
BseBI CCWGG 1 cut(s) 39
BseCI ATCGAT 1 cut(s) 507
BseDI CCNNGG 1 cut(s) 38
BseGI GGATG 4 cut(s) 10, 17, 178, 234
BseMI GCAATG 2 cut(s) 272, 463
BseSI GKGCMC 1 cut(s) 384
BshFI GGCC 2 cut(s) 100, 614
BshVI ATCGAT 1 cut(s) 507
BsnI GGCC 2 cut(s) 100, 614
Bsp1286I GDGCHC 1 cut(s) 384
Bsp143I GATC 4 cut(s) 142, 217, 289, 536
BspACI CCGC 1 cut(s) 315
BspANI GGCC 2 cut(s) 100, 614
BspDI ATCGAT 1 cut(s) 507
BspLI GGNNCC 1 cut(s) 219
BspMAI CTGCAG 1 cut(s) 139
BspPI GGATC 4 cut(s) 137, 212, 225, 531
BsrDI GCAATG 2 cut(s) 272, 463
BssECI CCNNGG 1 cut(s) 38
BssMI GATC 4 cut(s) 142, 217, 289, 536
Bst2UI CCWGG 1 cut(s) 39
Bst4CI ACNGT 4 cut(s) 274, 349, 523, 553
BstEII GGTNACC 1 cut(s) 488
BstF5I GGATG 4 cut(s) 10, 17, 178, 234
BstHHI GCGC 1 cut(s) 470
BstKTI GATC 4 cut(s) 145, 220, 292, 539
BstMBI GATC 4 cut(s) 142, 217, 289, 536
BstMWI GCNNNNNNNGC 1 cut(s) 276
BstNI CCWGG 1 cut(s) 39
BstPI GGTNACC 1 cut(s) 488
BstSCI CCNGG 1 cut(s) 37
BstSFI CTRYAG 1 cut(s) 135
BstSLI GKGCMC 1 cut(s) 384
BstX2I RGATCY 2 cut(s) 217, 536
BstYI RGATCY 2 cut(s) 217, 536
Bsu15I ATCGAT 1 cut(s) 507
BsuRI GGCC 2 cut(s) 100, 614
BsuTUI ATCGAT 1 cut(s) 507
BtsCI GGATG 4 cut(s) 10, 17, 178, 234
CaiI CAGNNNCTG 1 cut(s) 119
CfoI GCGC 1 cut(s) 470
Cfr13I GGNCC 1 cut(s) 99
ClaI ATCGAT 1 cut(s) 507
Csp6I GTAC 3 cut(s) 213, 275, 332
CviAII CATG 4 cut(s) 74, 302, 424, 500
CviJI RGCY 5 cut(s) 100, 300, 398, 498, 614
CviKI_1 RGCY 5 cut(s) 100, 300, 398, 498, 614
CviQI GTAC 3 cut(s) 213, 275, 332
DpnI GATC 4 cut(s) 144, 219, 291, 538
DpnII GATC 4 cut(s) 142, 217, 289, 536
EaeI YGGCCR 1 cut(s) 612
Eco32I GATATC 1 cut(s) 505
Eco57I CTGAAG 3 cut(s) 141, 384, 606
Eco91I GGTNACC 1 cut(s) 488
EcoO109I RGGNCCY 1 cut(s) 99
EcoO65I GGTNACC 1 cut(s) 488
EcoRII CCWGG 1 cut(s) 37
EcoRV GATATC 1 cut(s) 505
EcoT22I ATGCAT 1 cut(s) 660
FaeI CATG 4 cut(s) 77, 305, 427, 503
FalI AAGNNNNNCTT 2 cut(s) 114, 146
FatI CATG 4 cut(s) 73, 301, 423, 499
FauI CCCGC 1 cut(s) 322
FokI GGATG 4 cut(s) 4, 17, 185, 221
FspBI CTAG 1 cut(s) 69
FspI TGCGCA 1 cut(s) 469
GlaI GCGC 1 cut(s) 469
HaeIII GGCC 2 cut(s) 100, 614
HhaI GCGC 1 cut(s) 470
Hin1II CATG 4 cut(s) 77, 305, 427, 503
Hin6I GCGC 1 cut(s) 468
HinP1I GCGC 1 cut(s) 468
HincII GTYRAC 1 cut(s) 10
HindII GTYRAC 1 cut(s) 10
HindIII AAGCTT 1 cut(s) 396
HinfI GANTC 1 cut(s) 593
Hpy166II GTNNAC 3 cut(s) 10, 442, 451
Hpy188I TCNGA 1 cut(s) 598
Hpy188III TCNNGA 1 cut(s) 84
Hpy8I GTNNAC 3 cut(s) 10, 442, 451
HpyAV CCTTC 1 cut(s) 239
HpyCH4III ACNGT 4 cut(s) 274, 349, 523, 553
HpyCH4V TGCA 6 cut(s) 137, 270, 515, 572, 578, 658
HpyF10VI GCNNNNNNNGC 1 cut(s) 276
Hsp92II CATG 4 cut(s) 77, 305, 427, 503
HspAI GCGC 1 cut(s) 468
Kzo9I GATC 4 cut(s) 142, 217, 289, 536
LmnI GCTCC 2 cut(s) 262, 460
LpnPI CCDG 6 cut(s) 24, 51, 387, 555, 600, 609
LweI GCATC 3 cut(s) 479, 581, 587
MaeI CTAG 1 cut(s) 69
MaeIII GTNAC 2 cut(s) 42, 488
MalI GATC 4 cut(s) 144, 219, 291, 538
MboI GATC 4 cut(s) 142, 217, 289, 536
MboII GAAGA 2 cut(s) 134, 363
MflI RGATCY 2 cut(s) 217, 536
MhlI GDGCHC 1 cut(s) 384
MluCI AATT 3 cut(s) 154, 190, 429
MnlI CCTC 5 cut(s) 89, 90, 163, 373, 577
Mph1103I ATGCAT 1 cut(s) 660
MseI TTAA 1 cut(s) 661
MslI CAYNNNNRTG 3 cut(s) 285, 300, 498
MspR9I CCNGG 1 cut(s) 39
MvaI CCWGG 1 cut(s) 39
MwoI GCNNNNNNNGC 1 cut(s) 276
NdeII GATC 4 cut(s) 142, 217, 289, 536
NlaIII CATG 4 cut(s) 77, 305, 427, 503
NlaIV GGNNCC 1 cut(s) 219
NsbI TGCGCA 1 cut(s) 469
NsiI ATGCAT 1 cut(s) 660
PfeI GAWTC 1 cut(s) 593
PsiI TTATAA 1 cut(s) 558
Psp6I CCWGG 1 cut(s) 37
PspEI GGTNACC 1 cut(s) 488
PspGI CCWGG 1 cut(s) 37
PspN4I GGNNCC 1 cut(s) 219
PspPI GGNCC 1 cut(s) 99
PstI CTGCAG 1 cut(s) 139
PstNI CAGNNNCTG 1 cut(s) 119
PsuI RGATCY 2 cut(s) 217, 536
RsaI GTAC 3 cut(s) 214, 276, 333
RsaNI GTAC 3 cut(s) 213, 275, 332
RseI CAYNNNNRTG 3 cut(s) 285, 300, 498
SaqAI TTAA 1 cut(s) 661
Sau3AI GATC 4 cut(s) 142, 217, 289, 536
Sau96I GGNCC 1 cut(s) 99
ScrFI CCNGG 1 cut(s) 39
SduI GDGCHC 1 cut(s) 384
SetI ASST 7 cut(s) 40, 365, 400, 406, 520, 569, 628
SfaNI GCATC 3 cut(s) 479, 581, 587
SfcI CTRYAG 1 cut(s) 135
SmiMI CAYNNNNRTG 3 cut(s) 285, 300, 498
Sse9I AATT 3 cut(s) 154, 190, 429
SsiI CCGC 1 cut(s) 315
SspMI CTAG 1 cut(s) 69
StyD4I CCNGG 1 cut(s) 37
TaaI ACNGT 4 cut(s) 274, 349, 523, 553
TaqI TCGA 1 cut(s) 507
TasI AATT 3 cut(s) 154, 190, 429
TatI WGTACW 1 cut(s) 331
TfiI GAWTC 1 cut(s) 593
Tru1I TTAA 1 cut(s) 661
Tru9I TTAA 1 cut(s) 661
TspDTI ATGAA 4 cut(s) 72, 201, 318, 363
TspGWI ACGGA 2 cut(s) 230, 459
XspI CTAG 1 cut(s) 69
Zsp2I ATGCAT 1 cut(s) 660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.