Rh5BG562900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000990
Physical Location & Seq
Reverse (-)
89201305 .. 89202960
1656 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG562900.1

Sequence Viewer

Length: 1656 bp
ATGGAATTCTACCACAACCAACTCAAGCTTAGGTTATTAAATGAGAAGAAACCCTGTGTCTACAAAAGAGTTGATTGGCTAGTTGATAAGCTGGGTACCCAAGTGCATTCCTATTTCTGGCTTGATGAATACACTGAGAAGGATGATTTTGCACGATACTGGAAAAATGAGTGGGTGAGCGGTTTAACATCTTGGCGAACAGCTTTGGAGATTCCAGACTCTAATGTTGTCATTGAAGGTACATGTGCAAAAGTAACGGACCGGGTTGATCAAGAAAAGGCTTATGTTGTTTGGAACCCCGGTTCACAATTCAGTATTTGCAACTGCAGTTGGGCAGAAATGGGGAACCTGTGTGAGCATATACTCAAGGTTATTAGTGTCTGCAGGAAAAGAAGTCATCCCGTGCCATCTGTCAGCATATTGCAGTTCCACCAGGCCTTGCTTGATATGCTCCATTGCCCACCTCATGATTCTTTAATTCGTGATCACGCTGTTTCTTTAGCGGTCTTTGTACAGAATCAGTTAAATGGACCTAATTTGGAAAGCAGCAATAGCACAACGGATCAGGCAGCTTTCGCAGATAAAGATCTAGAATTAGTAAATGAGGGCCCAGTAATTGAAGAAGTATTATCTCATAATGAGAATAGTTGTGCGGATGAGCATGTTGCTGTGGGTACTGAAACAAGTAGTCTGGTTGCATGTGGAAATGGTATATATAATGCGGGTTGTGGAGATGAAATCACTTCTTCTGAGATGGATGTTGACCCATCATCCATCTGTATTTCTCCACCTGGGTTACATTCTGTTGATGAGGTTGTGTCTGGTGGTGTCCTCTCACGAAGCAGACAGAGGCCCTTGTTTAACACAGAAACTGAAGATCTGCTTCCCGCAGATGATCCTCTCGCTAAGTCAAGTGAATATGAGGATGATATGTTAGATGAAAATTGTGAAAAAAATGTGATGAGTACGGATCCACTCTCCCTTCATATCCCTTGTTCAGCAATGGAGTTTGTGGAGCAATACACAGTACCCCATTCTAATGATCTCCACAGCCATGAAGTCAATCCCGCCATTATTTCAAAAGTATGTGATGACAATACAGTATATATCAATACCTCCCCATCTTCATCTGTGCCTGTTGAACCACAAGCTTCAGGTGTCATAGAAAATGAGAGCATGGAAATTGAAAGTGTGGACGGAATGGACTTTGTGGAGCATTGCCCATTAACCAATCCTAATGGTAATCACAGCCATGATATCGATATTGCACCTACTGTTATTTCCAAAAGATCCCCTGACAATACCGTTTATAACAAGACCTCTGCATCTGCATCTATACCTGTTGAATCAGAAATGGTTGTACTGGCTGAAACCTCAGGTATCATTTTAGAAAATGGTAGAATGGAAATCGGAAGTGAGAATGGAAACACAAGTAAGCACAATCTGTCTGCTGATAATCCTTCCACCTTGGACGGCATTCGTGGTTGTATGATAAATGATTCAGATTTTAGTCAGGATTCAGAGTCAGTTGGCAATGTGATGGTTGTGTACCCAGAACCATTGCTCAAGTTCTCATCAACATCAAGTTGCAACAAGGAAGAGCTGCCTGTTGAGAATGGGGCCACTGAAACTGGGTCTAGTGAGAAGCCATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

551

Amino Acids

60.52

Weight (kDa)

4.47

Isoelectric Point (pI)

42.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1311
Acc65I GGTACC 1 cut(s) 95
AccB1I GGYRCC 1 cut(s) 95
AccBSI CCGCTC 1 cut(s) 180
AccI GTMKAC 1 cut(s) 60
AciI CCGC 6 cut(s) 180, 503, 653, 722, 888, 1068
AclWI GGATC 5 cut(s) 570, 890, 965, 978, 1284
AcsI RAATTY 1 cut(s) 5
AcuI CTGAAG 2 cut(s) 894, 1137
AfaI GTAC 8 cut(s) 97, 241, 513, 676, 967, 1029, 1362, 1550
AfiI CCNNNNNNNGG 1 cut(s) 117
AflIII ACRYGT 1 cut(s) 242
AgsI TTSAA 6 cut(s) 236, 620, 1080, 1142, 1187, 1346
AjnI CCWGG 2 cut(s) 432, 790
AloI GAACNNNNNNTCC 2 cut(s) 286, 318
AluBI AGCT 6 cut(s) 28, 91, 203, 572, 1151, 1603
AluI AGCT 6 cut(s) 28, 91, 203, 572, 1151, 1603
AlwI GGATC 5 cut(s) 570, 890, 965, 978, 1284
AlwNI CAGNNNCTG 1 cut(s) 872
AoxI GGCC 4 cut(s) 435, 607, 851, 1620
ApaI GGGCCC 1 cut(s) 611
ApeKI GCWGC 3 cut(s) 546, 569, 1603
ApoI RAATTY 1 cut(s) 5
Asp718I GGTACC 1 cut(s) 95
AspS9I GGNCC 6 cut(s) 259, 530, 607, 608, 852, 1620
AsuC2I CCSGG 2 cut(s) 263, 300
AsuHPI GGTGA 1 cut(s) 187
AvaII GGWCC 2 cut(s) 259, 530
AxyI CCTNAGG 1 cut(s) 1375
BaeGI GKGCMC 1 cut(s) 611
BaeI ACNNNNGTAYC 2 cut(s) 87, 120
BamHI GGATCC 1 cut(s) 970
BanI GGYRCC 1 cut(s) 95
BanII GRGCYC 1 cut(s) 611
BbvI GCAGC 3 cut(s) 558, 581, 1590
BccI CCATC 6 cut(s) 415, 748, 775, 782, 1129, 1534
BceAI ACGGC 1 cut(s) 1489
BciT130I CCWGG 2 cut(s) 434, 792
BclI TGATCA 2 cut(s) 268, 484
BcnI CCSGG 2 cut(s) 263, 300
BfaI CTAG 3 cut(s) 80, 590, 1638
BfmI CTRYAG 2 cut(s) 325, 382
BglII AGATCT 2 cut(s) 586, 877
BisI GCNGC 3 cut(s) 547, 570, 1604
BlsI GCNGC 3 cut(s) 548, 571, 1605
Bme1390I CCNGG 4 cut(s) 263, 300, 434, 792
Bme18I GGWCC 2 cut(s) 259, 530
BmgT120I GGNCC 6 cut(s) 259, 530, 607, 608, 852, 1620
BmiI GGNNCC 6 cut(s) 97, 296, 347, 609, 972, 1621
BmrFI CCNGG 4 cut(s) 263, 300, 434, 792
BmrI ACTGGG 2 cut(s) 605, 1641
BmsI GCATC 2 cut(s) 1334, 1340
BmuI ACTGGG 2 cut(s) 605, 1641
Bpu10I CCTNAGC 1 cut(s) 29
BpuEI CTTGAG 3 cut(s) 8, 350, 1550
BpuMI CCSGG 2 cut(s) 263, 300
Bsa29I ATCGAT 1 cut(s) 1260
BsaBI GATNNNNATC 1 cut(s) 585
BsaJI CCNNGG 3 cut(s) 298, 791, 1467
Bsc4I CCNNNNNNNGG 1 cut(s) 117
Bse1I ACTGG 4 cut(s) 164, 611, 1368, 1636
Bse21I CCTNAGG 1 cut(s) 1375
Bse3DI GCAATG 5 cut(s) 454, 1008, 1216, 1540, 1559
Bse8I GATNNNNATC 1 cut(s) 585
BseBI CCWGG 2 cut(s) 434, 792
BseCI ATCGAT 1 cut(s) 1260
BseDI CCNNGG 3 cut(s) 298, 791, 1467
BseGI GGATG 6 cut(s) 148, 397, 661, 763, 770, 931
BseJI GATNNNNATC 1 cut(s) 585
BseLI CCNNNNNNNGG 1 cut(s) 117
BseMI GCAATG 5 cut(s) 454, 1008, 1216, 1540, 1559
BseMII CTCAG 3 cut(s) 126, 741, 1389
BseNI ACTGG 4 cut(s) 164, 611, 1368, 1636
BseSI GKGCMC 1 cut(s) 611
BseXI GCAGC 3 cut(s) 558, 581, 1590
BseYI CCCAGC 1 cut(s) 91
BshFI GGCC 4 cut(s) 437, 609, 853, 1622
BshNI GGYRCC 1 cut(s) 95
BshVI ATCGAT 1 cut(s) 1260
BsiSI CCGG 2 cut(s) 262, 300
BslI CCNNNNNNNGG 1 cut(s) 117
BsmI GAATGC 2 cut(s) 106, 1476
BsnI GGCC 4 cut(s) 437, 609, 853, 1622
Bsp120I GGGCCC 1 cut(s) 607
Bsp1286I GDGCHC 1 cut(s) 611
Bsp1407I TGTACA 1 cut(s) 511
Bsp143I GATC 9 cut(s) 268, 484, 562, 586, 877, 895, 970, 1042, 1289
BspACI CCGC 6 cut(s) 180, 503, 653, 722, 888, 1068
BspANI GGCC 4 cut(s) 437, 609, 853, 1622
BspCNI CTCAG 3 cut(s) 127, 742, 1388
BspDI ATCGAT 1 cut(s) 1260
BspHI TCATGA 1 cut(s) 466
BspLI GGNNCC 6 cut(s) 97, 296, 347, 609, 972, 1621
BspMAI CTGCAG 2 cut(s) 329, 386
BspPI GGATC 5 cut(s) 570, 890, 965, 978, 1284
BspQI GCTCTTC 1 cut(s) 1593
BspT107I GGYRCC 1 cut(s) 95
BsrBI CCGCTC 1 cut(s) 180
BsrDI GCAATG 5 cut(s) 454, 1008, 1216, 1540, 1559
BsrGI TGTACA 1 cut(s) 511
BsrI ACTGG 4 cut(s) 164, 611, 1368, 1636
BssECI CCNNGG 3 cut(s) 298, 791, 1467
BssMI GATC 9 cut(s) 268, 484, 562, 586, 877, 895, 970, 1042, 1289
BssT1I CCWWGG 1 cut(s) 1467
Bst2UI CCWGG 2 cut(s) 434, 792
Bst4CI ACNGT 4 cut(s) 1027, 1102, 1276, 1306
Bst6I CTCTTC 1 cut(s) 1593
BstAUI TGTACA 1 cut(s) 511
BstDEI CTNAG 5 cut(s) 29, 135, 750, 906, 1375
BstF5I GGATG 6 cut(s) 148, 397, 661, 763, 770, 931
BstKTI GATC 9 cut(s) 271, 487, 565, 589, 880, 898, 973, 1045, 1292
BstMBI GATC 9 cut(s) 268, 484, 562, 586, 877, 895, 970, 1042, 1289
BstMWI GCNNNNNNNGC 3 cut(s) 448, 552, 575
BstNI CCWGG 2 cut(s) 434, 792
BstNSI RCATGY 3 cut(s) 246, 665, 702
BstSCI CCNGG 4 cut(s) 261, 298, 432, 790
BstSFI CTRYAG 2 cut(s) 325, 382
BstSLI GKGCMC 1 cut(s) 611
BstV1I GCAGC 3 cut(s) 558, 581, 1590
BstX2I RGATCY 4 cut(s) 586, 877, 970, 1289
BstYI RGATCY 4 cut(s) 586, 877, 970, 1289
Bsu15I ATCGAT 1 cut(s) 1260
Bsu36I CCTNAGG 1 cut(s) 1375
BsuRI GGCC 4 cut(s) 437, 609, 853, 1622
BsuTUI ATCGAT 1 cut(s) 1260
BtsCI GGATG 6 cut(s) 148, 397, 661, 763, 770, 931
BtsIMutI CAGTG 2 cut(s) 132, 1623
CaiI CAGNNNCTG 1 cut(s) 872
CciI TCATGA 1 cut(s) 466
Cfr13I GGNCC 6 cut(s) 259, 530, 607, 608, 852, 1620
ClaI ATCGAT 1 cut(s) 1260
CpoI CGGWCCG 1 cut(s) 259
Csp6I GTAC 8 cut(s) 96, 240, 512, 675, 966, 1028, 1361, 1549
CspI CGGWCCG 1 cut(s) 259
CviAII CATG 7 cut(s) 243, 467, 662, 699, 1055, 1177, 1253
CviQI GTAC 8 cut(s) 96, 240, 512, 675, 966, 1028, 1361, 1549
DdeI CTNAG 5 cut(s) 29, 135, 750, 906, 1375
DpnI GATC 9 cut(s) 270, 486, 564, 588, 879, 897, 972, 1044, 1291
DpnII GATC 9 cut(s) 268, 484, 562, 586, 877, 895, 970, 1042, 1289
Eam1104I CTCTTC 1 cut(s) 1593
EarI CTCTTC 1 cut(s) 1593
Eco130I CCWWGG 1 cut(s) 1467
Eco147I AGGCCT 1 cut(s) 437
Eco24I GRGCYC 1 cut(s) 611
Eco32I GATATC 1 cut(s) 1258
Eco47I GGWCC 2 cut(s) 259, 530
Eco57I CTGAAG 2 cut(s) 894, 1137
Eco81I CCTNAGG 1 cut(s) 1375
EcoO109I RGGNCCY 2 cut(s) 607, 852
EcoRI GAATTC 1 cut(s) 5
EcoRII CCWGG 2 cut(s) 432, 790
EcoRV GATATC 1 cut(s) 1258
EcoT14I CCWWGG 1 cut(s) 1467
EcoT38I GRGCYC 1 cut(s) 611
ErhI CCWWGG 1 cut(s) 1467
FaeI CATG 7 cut(s) 246, 470, 665, 702, 1058, 1180, 1256
FalI AAGNNNNNCTT 2 cut(s) 867, 899
FatI CATG 7 cut(s) 242, 466, 661, 698, 1054, 1176, 1252
FauI CCCGC 3 cut(s) 715, 895, 1075
FbaI TGATCA 2 cut(s) 268, 484
FblI GTMKAC 1 cut(s) 60
Fnu4HI GCNGC 3 cut(s) 547, 570, 1604
FokI GGATG 6 cut(s) 155, 384, 668, 757, 770, 938
FriOI GRGCYC 1 cut(s) 611
Fsp4HI GCNGC 3 cut(s) 547, 570, 1604
FspBI CTAG 3 cut(s) 80, 590, 1638
GluI GCNGC 3 cut(s) 547, 570, 1604
GsaI CCCAGC 1 cut(s) 95
HaeIII GGCC 4 cut(s) 437, 609, 853, 1622
HapII CCGG 2 cut(s) 262, 300
Hin1II CATG 7 cut(s) 246, 470, 665, 702, 1058, 1180, 1256
HincII GTYRAC 1 cut(s) 763
HindII GTYRAC 1 cut(s) 763
HindIII AAGCTT 2 cut(s) 26, 1149
HinfI GANTC 8 cut(s) 211, 218, 470, 517, 1346, 1499, 1517, 1523
HpaII CCGG 2 cut(s) 262, 300
HphI GGTGA 1 cut(s) 187
Hpy166II GTNNAC 5 cut(s) 61, 305, 763, 1195, 1549
Hpy188I TCNGA 5 cut(s) 751, 1351, 1412, 1504, 1522
Hpy188III TCNNGA 8 cut(s) 215, 272, 467, 482, 590, 837, 1514, 1653
Hpy8I GTNNAC 5 cut(s) 61, 305, 763, 1195, 1549
HpyAV CCTTC 4 cut(s) 133, 230, 992, 1470
HpyCH4III ACNGT 4 cut(s) 1027, 1102, 1276, 1306
HpyF10VI GCNNNNNNNGC 3 cut(s) 448, 552, 575
HpyF3I CTNAG 5 cut(s) 29, 135, 750, 906, 1375
Hsp92II CATG 7 cut(s) 246, 470, 665, 702, 1058, 1180, 1256
KpnI GGTACC 1 cut(s) 99
Ksp22I TGATCA 2 cut(s) 268, 484
Kzo9I GATC 9 cut(s) 268, 484, 562, 586, 877, 895, 970, 1042, 1289
LguI GCTCTTC 1 cut(s) 1593
LmnI GCTCC 3 cut(s) 456, 1015, 1213
Lsp1109I GCAGC 3 cut(s) 558, 581, 1590
LweI GCATC 2 cut(s) 1334, 1340
MaeI CTAG 3 cut(s) 80, 590, 1638
MaeIII GTNAC 2 cut(s) 253, 795
MalI GATC 9 cut(s) 270, 486, 564, 588, 879, 897, 972, 1044, 1291
MbiI CCGCTC 1 cut(s) 180
MboI GATC 9 cut(s) 268, 484, 562, 586, 877, 895, 970, 1042, 1289
MboII GAAGA 6 cut(s) 58, 632, 738, 887, 1116, 1610
MflI RGATCY 4 cut(s) 586, 877, 970, 1289
MhlI GDGCHC 1 cut(s) 611
MluCI AATT 8 cut(s) 5, 308, 477, 535, 593, 615, 943, 1182
MlyI GAGTC 2 cut(s) 212, 1532
MseI TTAA 6 cut(s) 38, 185, 476, 524, 861, 1226
MslI CAYNNNNRTG 3 cut(s) 1038, 1053, 1251
MspI CCGG 2 cut(s) 262, 300
MspR9I CCNGG 4 cut(s) 263, 300, 434, 792
Mva1269I GAATGC 2 cut(s) 106, 1476
MvaI CCWGG 2 cut(s) 434, 792
MwoI GCNNNNNNNGC 3 cut(s) 448, 552, 575
NciI CCSGG 2 cut(s) 263, 300
NdeII GATC 9 cut(s) 268, 484, 562, 586, 877, 895, 970, 1042, 1289
NlaIII CATG 7 cut(s) 246, 470, 665, 702, 1058, 1180, 1256
NlaIV GGNNCC 6 cut(s) 97, 296, 347, 609, 972, 1621
NspI RCATGY 3 cut(s) 246, 665, 702
PagI TCATGA 1 cut(s) 466
PceI AGGCCT 1 cut(s) 437
PciI ACATGT 1 cut(s) 242
PciSI GCTCTTC 1 cut(s) 1593
PctI GAATGC 2 cut(s) 106, 1476
PfeI GAWTC 6 cut(s) 211, 470, 517, 1346, 1499, 1517
PkrI GCNGC 3 cut(s) 548, 571, 1605
PleI GAGTC 2 cut(s) 212, 1531
PpsI GAGTC 2 cut(s) 212, 1531
PscI ACATGT 1 cut(s) 242
PsiI TTATAA 1 cut(s) 1311
Psp6I CCWGG 2 cut(s) 432, 790
PspFI CCCAGC 1 cut(s) 91
PspGI CCWGG 2 cut(s) 432, 790
PspN4I GGNNCC 6 cut(s) 97, 296, 347, 609, 972, 1621
PspOMI GGGCCC 1 cut(s) 607
PspPI GGNCC 6 cut(s) 259, 530, 607, 608, 852, 1620
PstI CTGCAG 2 cut(s) 329, 386
PstNI CAGNNNCTG 1 cut(s) 872
PsuI RGATCY 4 cut(s) 586, 877, 970, 1289
RsaI GTAC 8 cut(s) 97, 241, 513, 676, 967, 1029, 1362, 1550
RsaNI GTAC 8 cut(s) 96, 240, 512, 675, 966, 1028, 1361, 1549
RseI CAYNNNNRTG 3 cut(s) 1038, 1053, 1251
Rsr2I CGGWCCG 1 cut(s) 259
RsrII CGGWCCG 1 cut(s) 259
SapI GCTCTTC 1 cut(s) 1593
SaqAI TTAA 6 cut(s) 38, 185, 476, 524, 861, 1226
SatI GCNGC 3 cut(s) 547, 570, 1604
Sau3AI GATC 9 cut(s) 268, 484, 562, 586, 877, 895, 970, 1042, 1289
Sau96I GGNCC 6 cut(s) 259, 530, 607, 608, 852, 1620
SchI GAGTC 2 cut(s) 212, 1532
ScrFI CCNGG 4 cut(s) 263, 300, 434, 792
SduI GDGCHC 1 cut(s) 611
SfaNI GCATC 2 cut(s) 1334, 1340
SfcI CTRYAG 2 cut(s) 325, 382
SinI GGWCC 2 cut(s) 259, 530
SmiMI CAYNNNNRTG 3 cut(s) 1038, 1053, 1251
SmlI CTYRAG 3 cut(s) 23, 365, 1565
SmoI CTYRAG 3 cut(s) 23, 365, 1565
Sse9I AATT 8 cut(s) 5, 308, 477, 535, 593, 615, 943, 1182
SseBI AGGCCT 1 cut(s) 437
SsiI CCGC 6 cut(s) 180, 503, 653, 722, 888, 1068
SspMI CTAG 3 cut(s) 80, 590, 1638
StuI AGGCCT 1 cut(s) 437
StyD4I CCNGG 4 cut(s) 261, 298, 432, 790
StyI CCWWGG 1 cut(s) 1467
TaaI ACNGT 4 cut(s) 1027, 1102, 1276, 1306
TaqI TCGA 1 cut(s) 1260
TasI AATT 8 cut(s) 5, 308, 477, 535, 593, 615, 943, 1182
TatI WGTACW 2 cut(s) 511, 1360
TfiI GAWTC 6 cut(s) 211, 470, 517, 1346, 1499, 1517
Tru1I TTAA 6 cut(s) 38, 185, 476, 524, 861, 1226
Tru9I TTAA 6 cut(s) 38, 185, 476, 524, 861, 1226
TscAI CASTG 2 cut(s) 139, 1630
TseI GCWGC 3 cut(s) 546, 569, 1603
TspDTI ATGAA 6 cut(s) 141, 750, 954, 974, 1071, 1116
TspGWI ACGGA 4 cut(s) 272, 575, 983, 1212
TspRI CASTG 2 cut(s) 139, 1630
VpaK11BI GGWCC 2 cut(s) 259, 530
XapI RAATTY 1 cut(s) 5
XbaI TCTAGA 1 cut(s) 589
XceI RCATGY 3 cut(s) 246, 665, 702
XmiI GTMKAC 1 cut(s) 60
XspI CTAG 3 cut(s) 80, 590, 1638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.