Rmu_sc0006217.1_g000003

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006217.1
OG0000990
Physical Location & Seq
Forward (+)
13876 .. 15194
1319 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006217.1_g000003.1.cds

Sequence Viewer

Length: 1143 bp
atggctagatgggatgagattctgtctcttcctgtacagaatcctccggctttggagttttctgctgctgatcttgtgtggtcgaaggtgaaaggttggtgtgacaagaaagatggagttgctgtaatcccctatgccagagttgatgcttttgtgaggggtgaatcttcgaacaaagagtgtccgacgagatttcatgttgaagcgaggcggcggcggcaggcaaagacgaattgcaaaccaaaggttgatggcatccttgaatatattctatattggtgttcctttggtcctgatgaccacaggatgggcggctccatacgacccagtaggactaattatgtcccaaagaagaaaaattctggtcgaccaaacaccaagagaggttgtacctgccactttatggtgaaacgcttaattgctgaaccgtcagtagcacttcttgtatataattctgttaagcatgtggataagaaagggttgccatgccatggccctgaagataagatgtcttctggaacgcgcgctatgtttgccccatacatctctgaggatcaacgccttcgtgtgctatctttgctatatgtgggggtctccgtggagaccataatgcagagacatgctgaatctgtagagagacagggtggtccatgtaatcgtgatgacctcttaacccataggtacgttcgcagacaggagaggatcattcgacgttctacatatgaattggatgcggatgatgcagttagcatcagcatgtgggtggaaaaccatcagaatagcattttcttctatgagcatttctccgagacggaccctttcactttgggcattcaaacagactggcagctgcaacagatgattcgctttggcaatcacagccttatagcctctgattcaaggttcggaacaaacaaattaaagtatcctgttcacagtctccttgtatttaaccaagacaacaaggccattccggtagcatggatagtgagtccaaaatttgcaagttctgatacccataaatggatgagagctctttacaatagagttcagacgaaagatacttcatggaagttggctggcttcatagtggatgatcctcaggctgatgtacacactatcaggtgctcagtcattgtttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

380

Amino Acids

43.59

Weight (kDa)

9.3

Isoelectric Point (pI)

43.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 645
Acc36I ACCTGC 1 cut(s) 401
AccB7I CCANNNNNTGG 3 cut(s) 307, 403, 491
AccI GTMKAC 1 cut(s) 367
AccII CGCG 2 cut(s) 523, 525
AciI CCGC 5 cut(s) 211, 214, 217, 312, 734
AclWI GGATC 3 cut(s) 561, 710, 1091
AcsI RAATTY 2 cut(s) 358, 998
AcuI CTGAAG 1 cut(s) 519
AfaI GTAC 4 cut(s) 36, 391, 683, 1113
AfiI CCNNNNNNNGG 4 cut(s) 307, 403, 491, 1023
AgsI TTSAA 4 cut(s) 203, 263, 836, 900
AluBI AGCT 2 cut(s) 850, 1034
AluI AGCT 2 cut(s) 850, 1034
Alw21I GWGCWC 2 cut(s) 1036, 1130
Alw26I GTCTC 7 cut(s) 30, 596, 598, 610, 631, 803, 944
AlwI GGATC 3 cut(s) 561, 710, 1091
AoxI GGCC 2 cut(s) 493, 966
ApeKI GCWGC 3 cut(s) 65, 847, 850
ApoI RAATTY 2 cut(s) 358, 998
Asp700I GAANNNNTTC 1 cut(s) 267
AspLEI GCGC 2 cut(s) 525, 527
AspS9I GGNCC 4 cut(s) 290, 494, 647, 814
AsuHPI GGTGA 3 cut(s) 100, 173, 418
AsuII TTCGAA 1 cut(s) 170
AvaII GGWCC 3 cut(s) 290, 647, 814
AxyI CCTNAGG 1 cut(s) 1101
BanII GRGCYC 1 cut(s) 1036
BbsI GAAGAC 1 cut(s) 504
Bbv12I GWGCWC 2 cut(s) 1036, 1130
BbvI GCAGC 3 cut(s) 52, 837, 859
BccI CCATC 5 cut(s) 3, 107, 245, 301, 780
BciVI GTATCC 1 cut(s) 936
BcoDI GTCTC 7 cut(s) 30, 596, 598, 610, 631, 803, 944
BfaI CTAG 1 cut(s) 6
BfmI CTRYAG 1 cut(s) 630
BfuAI ACCTGC 1 cut(s) 401
BfuI GTATCC 1 cut(s) 936
BisI GCNGC 7 cut(s) 66, 212, 215, 218, 313, 848, 851
BlsI GCNGC 7 cut(s) 67, 213, 216, 219, 314, 849, 852
Bme18I GGWCC 3 cut(s) 290, 647, 814
BmgT120I GGNCC 4 cut(s) 290, 494, 647, 814
BmiI GGNNCC 2 cut(s) 316, 816
BmrI ACTGGG 1 cut(s) 321
BmsI GCATC 5 cut(s) 136, 264, 721, 730, 759
BmuI ACTGGG 1 cut(s) 321
BpiI GAAGAC 1 cut(s) 504
BplI GAGNNNNNCTC 2 cut(s) 788, 820
Bpu14I TTCGAA 1 cut(s) 170
BsaI GGTCTC 2 cut(s) 596, 598
BsaJI CCNNGG 2 cut(s) 490, 597
BsaWI WCCGGW 1 cut(s) 973
BsaXI ACNNNNNCTCC 2 cut(s) 108, 138
Bsc4I CCNNNNNNNGG 4 cut(s) 307, 403, 491, 1023
Bse1I ACTGG 2 cut(s) 327, 848
Bse21I CCTNAGG 1 cut(s) 1101
BseDI CCNNGG 2 cut(s) 490, 597
BseGI GGATG 7 cut(s) 19, 255, 312, 736, 742, 1032, 1099
BseLI CCNNNNNNNGG 4 cut(s) 307, 403, 491, 1023
BseMII CTCAG 3 cut(s) 540, 1115, 1143
BseNI ACTGG 2 cut(s) 327, 848
BsePI GCGCGC 1 cut(s) 523
BseXI GCAGC 3 cut(s) 52, 837, 859
Bsh1236I CGCG 2 cut(s) 523, 525
BshFI GGCC 2 cut(s) 495, 968
BsiHKAI GWGCWC 2 cut(s) 1036, 1130
BsiSI CCGG 2 cut(s) 47, 974
BslFI GGGAC 1 cut(s) 329
BslI CCNNNNNNNGG 4 cut(s) 307, 403, 491, 1023
BsmAI GTCTC 7 cut(s) 30, 596, 598, 610, 631, 803, 944
BsmBI CGTCTC 1 cut(s) 803
BsmFI GGGAC 1 cut(s) 329
BsmI GAATGC 1 cut(s) 831
BsnI GGCC 2 cut(s) 495, 968
Bso31I GGTCTC 2 cut(s) 596, 598
Bsp119I TTCGAA 1 cut(s) 170
Bsp1286I GDGCHC 2 cut(s) 1036, 1130
Bsp1407I TGTACA 2 cut(s) 34, 1111
Bsp143I GATC 4 cut(s) 70, 553, 702, 1096
Bsp19I CCATGG 1 cut(s) 490
BspACI CCGC 5 cut(s) 211, 214, 217, 312, 734
BspANI GGCC 2 cut(s) 495, 968
BspCNI CTCAG 3 cut(s) 541, 1114, 1142
BspFNI CGCG 2 cut(s) 523, 525
BspLI GGNNCC 2 cut(s) 316, 816
BspMI ACCTGC 1 cut(s) 401
BspPI GGATC 3 cut(s) 561, 710, 1091
BspT104I TTCGAA 1 cut(s) 170
BspTNI GGTCTC 2 cut(s) 596, 598
BsrGI TGTACA 2 cut(s) 34, 1111
BsrI ACTGG 2 cut(s) 327, 848
BssECI CCNNGG 2 cut(s) 490, 597
BssHII GCGCGC 1 cut(s) 523
BssMI GATC 4 cut(s) 70, 553, 702, 1096
BssT1I CCWWGG 1 cut(s) 490
Bst4CI ACNGT 2 cut(s) 429, 938
Bst6I CTCTTC 1 cut(s) 33
BstAUI TGTACA 2 cut(s) 34, 1111
BstBI TTCGAA 1 cut(s) 170
BstC8I GCNNGC 3 cut(s) 222, 525, 1081
BstDEI CTNAG 3 cut(s) 549, 1101, 1129
BstDSI CCRYGG 2 cut(s) 490, 597
BstF5I GGATG 7 cut(s) 19, 255, 312, 736, 742, 1032, 1099
BstFNI CGCG 2 cut(s) 523, 525
BstHHI GCGC 2 cut(s) 525, 527
BstKTI GATC 4 cut(s) 73, 556, 705, 1099
BstMAI GTCTC 7 cut(s) 30, 596, 598, 610, 631, 803, 944
BstMBI GATC 4 cut(s) 70, 553, 702, 1096
BstMWI GCNNNNNNNGC 5 cut(s) 217, 533, 577, 740, 879
BstNSI RCATGY 3 cut(s) 467, 623, 760
BstSFI CTRYAG 1 cut(s) 630
BstUI CGCG 2 cut(s) 523, 525
BstV1I GCAGC 3 cut(s) 52, 837, 859
BstV2I GAAGAC 1 cut(s) 504
Bsu36I CCTNAGG 1 cut(s) 1101
BsuI GTATCC 1 cut(s) 936
BsuRI GGCC 2 cut(s) 495, 968
BtgI CCRYGG 2 cut(s) 490, 597
BtsCI GGATG 7 cut(s) 19, 255, 312, 736, 742, 1032, 1099
BveI ACCTGC 1 cut(s) 401
Cac8I GCNNGC 3 cut(s) 222, 525, 1081
CfoI GCGC 2 cut(s) 525, 527
Cfr13I GGNCC 4 cut(s) 290, 494, 647, 814
Csp6I GTAC 4 cut(s) 35, 390, 682, 1112
CviAII CATG 9 cut(s) 197, 464, 486, 491, 620, 651, 757, 981, 1068
CviQI GTAC 4 cut(s) 35, 390, 682, 1112
DdeI CTNAG 3 cut(s) 549, 1101, 1129
DpnI GATC 4 cut(s) 72, 555, 704, 1098
DpnII GATC 4 cut(s) 70, 553, 702, 1096
DrdI GACNNNNNNGTC 1 cut(s) 645
DseDI GACNNNNNNGTC 1 cut(s) 645
Eam1104I CTCTTC 1 cut(s) 33
EarI CTCTTC 1 cut(s) 33
Ecl136II GAGCTC 1 cut(s) 1034
Eco130I CCWWGG 1 cut(s) 490
Eco24I GRGCYC 1 cut(s) 1036
Eco31I GGTCTC 2 cut(s) 596, 598
Eco47I GGWCC 3 cut(s) 290, 647, 814
Eco53kI GAGCTC 1 cut(s) 1034
Eco57I CTGAAG 1 cut(s) 519
Eco81I CCTNAGG 1 cut(s) 1101
EcoICRI GAGCTC 1 cut(s) 1034
EcoT14I CCWWGG 1 cut(s) 490
EcoT38I GRGCYC 1 cut(s) 1036
ErhI CCWWGG 1 cut(s) 490
Esp3I CGTCTC 1 cut(s) 803
FaeI CATG 9 cut(s) 200, 467, 489, 494, 623, 654, 760, 984, 1071
FaqI GGGAC 1 cut(s) 329
FatI CATG 9 cut(s) 196, 463, 485, 490, 619, 650, 756, 980, 1067
FauNDI CATATG 1 cut(s) 721
FblI GTMKAC 1 cut(s) 367
Fnu4HI GCNGC 7 cut(s) 66, 212, 215, 218, 313, 848, 851
FokI GGATG 7 cut(s) 26, 242, 319, 743, 749, 1039, 1106
FriOI GRGCYC 1 cut(s) 1036
Fsp4HI GCNGC 7 cut(s) 66, 212, 215, 218, 313, 848, 851
FspBI CTAG 1 cut(s) 6
GlaI GCGC 2 cut(s) 524, 526
GluI GCNGC 7 cut(s) 66, 212, 215, 218, 313, 848, 851
HaeIII GGCC 2 cut(s) 495, 968
HapII CCGG 2 cut(s) 47, 974
HhaI GCGC 2 cut(s) 525, 527
Hin1II CATG 9 cut(s) 200, 467, 489, 494, 623, 654, 760, 984, 1071
Hin6I GCGC 2 cut(s) 523, 525
HinP1I GCGC 2 cut(s) 523, 525
HincII GTYRAC 1 cut(s) 368
HindII GTYRAC 1 cut(s) 368
HinfI GANTC 7 cut(s) 19, 40, 164, 626, 862, 896, 991
HpaII CCGG 2 cut(s) 47, 974
HphI GGTGA 3 cut(s) 100, 173, 418
Hpy166II GTNNAC 3 cut(s) 368, 934, 1114
Hpy188I TCNGA 8 cut(s) 186, 550, 777, 808, 895, 908, 1012, 1053
Hpy188III TCNNGA 3 cut(s) 293, 516, 659
Hpy8I GTNNAC 3 cut(s) 368, 934, 1114
Hpy99I CGWCG 2 cut(s) 190, 714
HpyAV CCTTC 2 cut(s) 79, 572
HpyCH4III ACNGT 2 cut(s) 429, 938
HpyCH4IV ACGT 2 cut(s) 684, 712
HpyCH4V TGCA 5 cut(s) 237, 613, 743, 853, 1004
HpyF10VI GCNNNNNNNGC 5 cut(s) 217, 533, 577, 740, 879
HpyF3I CTNAG 3 cut(s) 549, 1101, 1129
HpySE526I ACGT 2 cut(s) 684, 712
Hsp92II CATG 9 cut(s) 200, 467, 489, 494, 623, 654, 760, 984, 1071
HspAI GCGC 2 cut(s) 523, 525
Kzo9I GATC 4 cut(s) 70, 553, 702, 1096
LmnI GCTCC 1 cut(s) 320
Lsp1109I GCAGC 3 cut(s) 52, 837, 859
LweI GCATC 5 cut(s) 136, 264, 721, 730, 759
MaeI CTAG 1 cut(s) 6
MaeII ACGT 2 cut(s) 684, 712
MaeIII GTNAC 1 cut(s) 101
MalI GATC 4 cut(s) 72, 555, 704, 1098
MboI GATC 4 cut(s) 70, 553, 702, 1096
MboII GAAGA 6 cut(s) 20, 159, 364, 504, 512, 781
MhlI GDGCHC 2 cut(s) 1036, 1130
MluCI AATT 8 cut(s) 232, 337, 358, 417, 451, 725, 917, 998
MlyI GAGTC 1 cut(s) 1000
MmeI TCCRAC 1 cut(s) 209
MnlI CCTC 9 cut(s) 54, 150, 201, 377, 544, 677, 693, 901, 1110
MroXI GAANNNNTTC 1 cut(s) 267
MseI TTAA 6 cut(s) 416, 459, 671, 920, 951, 1141
MslI CAYNNNNRTG 2 cut(s) 755, 761
MspA1I CMGCKG 1 cut(s) 850
MspI CCGG 2 cut(s) 47, 974
Mva1269I GAATGC 1 cut(s) 831
MvnI CGCG 2 cut(s) 523, 525
MwoI GCNNNNNNNGC 5 cut(s) 217, 533, 577, 740, 879
NcoI CCATGG 1 cut(s) 490
NdeI CATATG 1 cut(s) 721
NdeII GATC 4 cut(s) 70, 553, 702, 1096
NlaIII CATG 9 cut(s) 200, 467, 489, 494, 623, 654, 760, 984, 1071
NlaIV GGNNCC 2 cut(s) 316, 816
NmuCI GTSAC 1 cut(s) 101
NspI RCATGY 3 cut(s) 467, 623, 760
NspV TTCGAA 1 cut(s) 170
PauI GCGCGC 1 cut(s) 523
PctI GAATGC 1 cut(s) 831
PdmI GAANNNNTTC 1 cut(s) 267
PfeI GAWTC 6 cut(s) 19, 40, 164, 626, 862, 896
PflMI CCANNNNNTGG 3 cut(s) 307, 403, 491
PkrI GCNGC 7 cut(s) 67, 213, 216, 219, 314, 849, 852
PleI GAGTC 1 cut(s) 999
PpsI GAGTC 1 cut(s) 999
Psp124BI GAGCTC 1 cut(s) 1036
PspN4I GGNNCC 2 cut(s) 316, 816
PspPI GGNCC 4 cut(s) 290, 494, 647, 814
PteI GCGCGC 1 cut(s) 523
PvuII CAGCTG 1 cut(s) 850
RsaI GTAC 4 cut(s) 36, 391, 683, 1113
RsaNI GTAC 4 cut(s) 35, 390, 682, 1112
RseI CAYNNNNRTG 2 cut(s) 755, 761
SacI GAGCTC 1 cut(s) 1036
SalI GTCGAC 1 cut(s) 366
SaqAI TTAA 6 cut(s) 416, 459, 671, 920, 951, 1141
SatI GCNGC 7 cut(s) 66, 212, 215, 218, 313, 848, 851
Sau3AI GATC 4 cut(s) 70, 553, 702, 1096
Sau96I GGNCC 4 cut(s) 290, 494, 647, 814
SchI GAGTC 1 cut(s) 1000
SduI GDGCHC 2 cut(s) 1036, 1130
SfaNI GCATC 5 cut(s) 136, 264, 721, 730, 759
SfcI CTRYAG 1 cut(s) 630
SfuI TTCGAA 1 cut(s) 170
SinI GGWCC 3 cut(s) 290, 647, 814
SmiMI CAYNNNNRTG 2 cut(s) 755, 761
Sse9I AATT 8 cut(s) 232, 337, 358, 417, 451, 725, 917, 998
SsiI CCGC 5 cut(s) 211, 214, 217, 312, 734
SspMI CTAG 1 cut(s) 6
SstI GAGCTC 1 cut(s) 1036
StyI CCWWGG 1 cut(s) 490
TaaI ACNGT 2 cut(s) 429, 938
TaiI ACGT 2 cut(s) 687, 715
TaqI TCGA 4 cut(s) 83, 170, 367, 709
TasI AATT 8 cut(s) 232, 337, 358, 417, 451, 725, 917, 998
TatI WGTACW 2 cut(s) 34, 1111
TauI GCSGC 4 cut(s) 214, 217, 220, 315
TfiI GAWTC 6 cut(s) 19, 40, 164, 626, 862, 896
Tru1I TTAA 6 cut(s) 416, 459, 671, 920, 951, 1141
Tru9I TTAA 6 cut(s) 416, 459, 671, 920, 951, 1141
TseFI GTSAC 1 cut(s) 101
TseI GCWGC 3 cut(s) 65, 847, 850
Tsp45I GTSAC 1 cut(s) 101
TspDTI ATGAA 4 cut(s) 185, 738, 1056, 1075
TspGWI ACGGA 2 cut(s) 586, 827
Van91I CCANNNNNTGG 3 cut(s) 307, 403, 491
VpaK11BI GGWCC 3 cut(s) 290, 647, 814
XapI RAATTY 2 cut(s) 358, 998
XceI RCATGY 3 cut(s) 467, 623, 760
XmiI GTMKAC 1 cut(s) 367
XmnI GAANNNNTTC 1 cut(s) 267
XspI CTAG 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.