Rh5BG563000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000990
Physical Location & Seq
Reverse (-)
89202980 .. 89206447
3468 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG563000.1

Sequence Viewer

Length: 1224 bp
ATGGACCTGTTTTGCAAATACTCGCCGGTTTCCCTCCCTATTTCGCCTTCTCTTTTGCAGATGGCTAGATGGGATGAGATTCTGTCTCTTCCTGTACAGAATCCTCCGACTTTGGAGTTTTCTGCTGCTGATCTTGTGTGGTCGAAGGTGGAAGGTTGGCGTGACAAGAAAGATAGAGTTGCTGTGATCCCCTATGCCAGAGTTGATGCTTTTGTGAGGGGTGAATCTTCCAACAAAGAGTGTCCGACGAGATTTCATGTTGAAGCGAGGCGGCGGCGGCAGGCAAAGACGAATTGCAAACCAAAGGTTGATGGCATCCTTGAATATATTCTATATTGGTGTTCCTTTGGTCCTGATGACCACAGGATGGGCGGCTCCATACGACCCAGTAGGACTAATTATGTCCCAAAGAAGAAAAATTCTGGTCGACCAAACACCAAGAGAGGTTGTACCTGCCACTTTATGGTGAAACGCTTAATTGTTGAACCGTCAGTAGCGCTTCTTGTATATAATTCTGTTAAGCATGTGGATAAGAAAGGGTTGCCATGCCATGGCCCTGAAGATAAGATGGCTTCTGGAACGCGCGCTATGTTTGCCCCATACATCTCTGAGGATCTACGCCTTCGTGTGCTATCTTTGCTATATGTGGGGGTCTCCGTGGAGACCATAATGCAGAGACATGCTGAATCTGTAGAGAGACAGGGTGGTCCATGTAATCGTGATGACCTCTTAACCCATAGGTACGTTCGCAGACAGGAGAGGATCATTCGACGTTCTACATATGAATTGGATGCGGATGATGCAGTTAGCATCGGCATGTGGGTGGAAAACCATCAGAATATCATTTTCTTCTATGAGGATTTCTCTGAGACGGACCCTTTCACTTTGGGCATTCAAACAGACTGGCAGCTGCAACAGATGATTCGCTTTGGCAATCACAGCCTTATAGCCTCTGATTCAAGGTTTGGAACAAACAAATTAAAGTATCCTGTTCACAGTCTCCTTGTATTTAACCAAGACAACAAGGCCATTCCGGTAGCATGGATAGTGAGTCCAAAATTTGCAAGTTCTGATACCCATAAATGGATGAGAGCTCTTTACAATAGAGTTCAGACGAAAGATACTTCATGGAAGTTGGCTGGCTTCATAGTGGATGATCCTCAGGCTGATGTACACACTATCAGGGAAGTGGACTACTGCATTACAAAGTCTTCCTCTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

407

Amino Acids

46.81

Weight (kDa)

9.14

Isoelectric Point (pI)

46.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 705
Acc36I ACCTGC 1 cut(s) 461
AccB7I CCANNNNNTGG 3 cut(s) 367, 463, 551
AccI GTMKAC 1 cut(s) 427
AccII CGCG 2 cut(s) 583, 585
AciI CCGC 5 cut(s) 271, 274, 277, 372, 794
AclWI GGATC 4 cut(s) 181, 621, 770, 1151
AcsI RAATTY 2 cut(s) 418, 1058
AcuI CTGAAG 1 cut(s) 579
AfaI GTAC 4 cut(s) 96, 451, 743, 1173
AfeI AGCGCT 1 cut(s) 498
AfiI CCNNNNNNNGG 4 cut(s) 367, 463, 551, 1083
AgsI TTSAA 5 cut(s) 263, 323, 485, 896, 960
AluBI AGCT 2 cut(s) 910, 1094
AluI AGCT 2 cut(s) 910, 1094
Alw21I GWGCWC 1 cut(s) 1096
Alw26I GTCTC 7 cut(s) 90, 656, 658, 670, 691, 863, 1004
AlwI GGATC 4 cut(s) 181, 621, 770, 1151
Aor51HI AGCGCT 1 cut(s) 498
AoxI GGCC 2 cut(s) 553, 1026
ApeKI GCWGC 3 cut(s) 125, 907, 910
ApoI RAATTY 2 cut(s) 418, 1058
Asp700I GAANNNNTTC 1 cut(s) 327
AspLEI GCGC 3 cut(s) 499, 585, 587
AspS9I GGNCC 5 cut(s) 4, 350, 554, 707, 874
AsuHPI GGTGA 2 cut(s) 233, 478
AvaII GGWCC 4 cut(s) 4, 350, 707, 874
AxyI CCTNAGG 1 cut(s) 1161
BanII GRGCYC 1 cut(s) 1096
BbsI GAAGAC 1 cut(s) 1203
Bbv12I GWGCWC 1 cut(s) 1096
BbvI GCAGC 3 cut(s) 112, 897, 919
BccI CCATC 6 cut(s) 55, 63, 305, 361, 562, 840
BciVI GTATCC 1 cut(s) 996
BcoDI GTCTC 7 cut(s) 90, 656, 658, 670, 691, 863, 1004
BfaI CTAG 2 cut(s) 66, 1222
BfmI CTRYAG 1 cut(s) 690
BfoI RGCGCY 1 cut(s) 500
BfuAI ACCTGC 1 cut(s) 461
BfuI GTATCC 1 cut(s) 996
BisI GCNGC 7 cut(s) 126, 272, 275, 278, 373, 908, 911
BlsI GCNGC 7 cut(s) 127, 273, 276, 279, 374, 909, 912
Bme18I GGWCC 4 cut(s) 4, 350, 707, 874
BmgT120I GGNCC 5 cut(s) 4, 350, 554, 707, 874
BmiI GGNNCC 2 cut(s) 376, 876
BmrI ACTGGG 1 cut(s) 381
BmsI GCATC 5 cut(s) 196, 324, 781, 790, 819
BmuI ACTGGG 1 cut(s) 381
BpiI GAAGAC 1 cut(s) 1203
BplI GAGNNNNNCTC 2 cut(s) 848, 880
BsaI GGTCTC 2 cut(s) 656, 658
BsaJI CCNNGG 2 cut(s) 550, 657
BsaWI WCCGGW 1 cut(s) 1033
Bsc4I CCNNNNNNNGG 4 cut(s) 367, 463, 551, 1083
Bse118I RCCGGY 1 cut(s) 25
Bse1I ACTGG 2 cut(s) 387, 908
Bse21I CCTNAGG 1 cut(s) 1161
BseDI CCNNGG 2 cut(s) 550, 657
BseGI GGATG 7 cut(s) 79, 315, 372, 796, 802, 1092, 1159
BseLI CCNNNNNNNGG 4 cut(s) 367, 463, 551, 1083
BseMII CTCAG 3 cut(s) 600, 858, 1175
BseNI ACTGG 2 cut(s) 387, 908
BsePI GCGCGC 1 cut(s) 583
BseXI GCAGC 3 cut(s) 112, 897, 919
Bsh1236I CGCG 2 cut(s) 583, 585
BshFI GGCC 2 cut(s) 555, 1028
BsiHKAI GWGCWC 1 cut(s) 1096
BsiSI CCGG 2 cut(s) 26, 1034
BslFI GGGAC 1 cut(s) 389
BslI CCNNNNNNNGG 4 cut(s) 367, 463, 551, 1083
BsmAI GTCTC 7 cut(s) 90, 656, 658, 670, 691, 863, 1004
BsmBI CGTCTC 1 cut(s) 863
BsmFI GGGAC 1 cut(s) 389
BsmI GAATGC 1 cut(s) 891
BsnI GGCC 2 cut(s) 555, 1028
Bso31I GGTCTC 2 cut(s) 656, 658
Bsp1286I GDGCHC 1 cut(s) 1096
Bsp1407I TGTACA 2 cut(s) 94, 1171
Bsp143I GATC 5 cut(s) 130, 186, 613, 762, 1156
Bsp19I CCATGG 1 cut(s) 550
BspACI CCGC 5 cut(s) 271, 274, 277, 372, 794
BspANI GGCC 2 cut(s) 555, 1028
BspCNI CTCAG 3 cut(s) 601, 859, 1174
BspFNI CGCG 2 cut(s) 583, 585
BspLI GGNNCC 2 cut(s) 376, 876
BspMI ACCTGC 1 cut(s) 461
BspPI GGATC 4 cut(s) 181, 621, 770, 1151
BspTNI GGTCTC 2 cut(s) 656, 658
BsrFI RCCGGY 1 cut(s) 25
BsrGI TGTACA 2 cut(s) 94, 1171
BsrI ACTGG 2 cut(s) 387, 908
BssAI RCCGGY 1 cut(s) 25
BssECI CCNNGG 2 cut(s) 550, 657
BssHII GCGCGC 1 cut(s) 583
BssMI GATC 5 cut(s) 130, 186, 613, 762, 1156
BssT1I CCWWGG 1 cut(s) 550
Bst4CI ACNGT 2 cut(s) 489, 998
Bst6I CTCTTC 1 cut(s) 93
BstAUI TGTACA 2 cut(s) 94, 1171
BstC8I GCNNGC 3 cut(s) 282, 585, 1141
BstDEI CTNAG 3 cut(s) 609, 867, 1161
BstDSI CCRYGG 2 cut(s) 550, 657
BstF5I GGATG 7 cut(s) 79, 315, 372, 796, 802, 1092, 1159
BstFNI CGCG 2 cut(s) 583, 585
BstH2I RGCGCY 1 cut(s) 500
BstHHI GCGC 3 cut(s) 499, 585, 587
BstKTI GATC 5 cut(s) 133, 189, 616, 765, 1159
BstMAI GTCTC 7 cut(s) 90, 656, 658, 670, 691, 863, 1004
BstMBI GATC 5 cut(s) 130, 186, 613, 762, 1156
BstMWI GCNNNNNNNGC 5 cut(s) 277, 593, 637, 800, 939
BstNSI RCATGY 3 cut(s) 527, 683, 820
BstSFI CTRYAG 1 cut(s) 690
BstUI CGCG 2 cut(s) 583, 585
BstV1I GCAGC 3 cut(s) 112, 897, 919
BstV2I GAAGAC 1 cut(s) 1203
BstX2I RGATCY 1 cut(s) 613
BstYI RGATCY 1 cut(s) 613
Bsu36I CCTNAGG 1 cut(s) 1161
BsuI GTATCC 1 cut(s) 996
BsuRI GGCC 2 cut(s) 555, 1028
BtgI CCRYGG 2 cut(s) 550, 657
BtsCI GGATG 7 cut(s) 79, 315, 372, 796, 802, 1092, 1159
BveI ACCTGC 1 cut(s) 461
Cac8I GCNNGC 3 cut(s) 282, 585, 1141
CfoI GCGC 3 cut(s) 499, 585, 587
Cfr10I RCCGGY 1 cut(s) 25
Cfr13I GGNCC 5 cut(s) 4, 350, 554, 707, 874
Csp6I GTAC 4 cut(s) 95, 450, 742, 1172
CviAII CATG 9 cut(s) 257, 524, 546, 551, 680, 711, 817, 1041, 1128
CviQI GTAC 4 cut(s) 95, 450, 742, 1172
DdeI CTNAG 3 cut(s) 609, 867, 1161
DpnI GATC 5 cut(s) 132, 188, 615, 764, 1158
DpnII GATC 5 cut(s) 130, 186, 613, 762, 1156
DrdI GACNNNNNNGTC 1 cut(s) 705
DseDI GACNNNNNNGTC 1 cut(s) 705
Eam1104I CTCTTC 1 cut(s) 93
EarI CTCTTC 1 cut(s) 93
Ecl136II GAGCTC 1 cut(s) 1094
Eco130I CCWWGG 1 cut(s) 550
Eco24I GRGCYC 1 cut(s) 1096
Eco31I GGTCTC 2 cut(s) 656, 658
Eco47I GGWCC 4 cut(s) 4, 350, 707, 874
Eco47III AGCGCT 1 cut(s) 498
Eco53kI GAGCTC 1 cut(s) 1094
Eco57I CTGAAG 1 cut(s) 579
Eco81I CCTNAGG 1 cut(s) 1161
EcoICRI GAGCTC 1 cut(s) 1094
EcoT14I CCWWGG 1 cut(s) 550
EcoT38I GRGCYC 1 cut(s) 1096
ErhI CCWWGG 1 cut(s) 550
Esp3I CGTCTC 1 cut(s) 863
FaeI CATG 9 cut(s) 260, 527, 549, 554, 683, 714, 820, 1044, 1131
FaqI GGGAC 1 cut(s) 389
FatI CATG 9 cut(s) 256, 523, 545, 550, 679, 710, 816, 1040, 1127
FauNDI CATATG 1 cut(s) 781
FblI GTMKAC 1 cut(s) 427
Fnu4HI GCNGC 7 cut(s) 126, 272, 275, 278, 373, 908, 911
FokI GGATG 7 cut(s) 86, 302, 379, 803, 809, 1099, 1166
FriOI GRGCYC 1 cut(s) 1096
Fsp4HI GCNGC 7 cut(s) 126, 272, 275, 278, 373, 908, 911
FspBI CTAG 2 cut(s) 66, 1222
GlaI GCGC 3 cut(s) 498, 584, 586
GluI GCNGC 7 cut(s) 126, 272, 275, 278, 373, 908, 911
HaeII RGCGCY 1 cut(s) 500
HaeIII GGCC 2 cut(s) 555, 1028
HapII CCGG 2 cut(s) 26, 1034
HhaI GCGC 3 cut(s) 499, 585, 587
Hin1II CATG 9 cut(s) 260, 527, 549, 554, 683, 714, 820, 1044, 1131
Hin6I GCGC 3 cut(s) 497, 583, 585
HinP1I GCGC 3 cut(s) 497, 583, 585
HincII GTYRAC 1 cut(s) 428
HindII GTYRAC 1 cut(s) 428
HinfI GANTC 7 cut(s) 79, 100, 224, 686, 922, 956, 1051
HpaII CCGG 2 cut(s) 26, 1034
HphI GGTGA 2 cut(s) 233, 478
Hpy166II GTNNAC 4 cut(s) 428, 994, 1174, 1192
Hpy188I TCNGA 8 cut(s) 108, 246, 610, 837, 868, 955, 1072, 1113
Hpy188III TCNNGA 3 cut(s) 353, 576, 719
Hpy8I GTNNAC 4 cut(s) 428, 994, 1174, 1192
Hpy99I CGWCG 2 cut(s) 250, 774
HpyAV CCTTC 4 cut(s) 57, 139, 146, 632
HpyCH4III ACNGT 2 cut(s) 489, 998
HpyCH4IV ACGT 2 cut(s) 744, 772
HpyCH4V TGCA 8 cut(s) 15, 58, 297, 673, 803, 913, 1064, 1200
HpyF10VI GCNNNNNNNGC 5 cut(s) 277, 593, 637, 800, 939
HpyF3I CTNAG 3 cut(s) 609, 867, 1161
HpySE526I ACGT 2 cut(s) 744, 772
Hsp92II CATG 9 cut(s) 260, 527, 549, 554, 683, 714, 820, 1044, 1131
HspAI GCGC 3 cut(s) 497, 583, 585
Kzo9I GATC 5 cut(s) 130, 186, 613, 762, 1156
LmnI GCTCC 1 cut(s) 380
Lsp1109I GCAGC 3 cut(s) 112, 897, 919
LweI GCATC 5 cut(s) 196, 324, 781, 790, 819
MaeI CTAG 2 cut(s) 66, 1222
MaeII ACGT 2 cut(s) 744, 772
MaeIII GTNAC 1 cut(s) 161
MalI GATC 5 cut(s) 132, 188, 615, 764, 1158
MboI GATC 5 cut(s) 130, 186, 613, 762, 1156
MboII GAAGA 6 cut(s) 80, 219, 424, 572, 841, 1203
MflI RGATCY 1 cut(s) 613
MhlI GDGCHC 1 cut(s) 1096
MluCI AATT 8 cut(s) 292, 397, 418, 477, 511, 785, 977, 1058
MlyI GAGTC 1 cut(s) 1060
MmeI TCCRAC 3 cut(s) 131, 255, 269
MroXI GAANNNNTTC 1 cut(s) 327
MseI TTAA 5 cut(s) 476, 519, 731, 980, 1011
MslI CAYNNNNRTG 2 cut(s) 815, 821
MspA1I CMGCKG 1 cut(s) 910
MspI CCGG 2 cut(s) 26, 1034
Mva1269I GAATGC 1 cut(s) 891
MvnI CGCG 2 cut(s) 583, 585
MwoI GCNNNNNNNGC 5 cut(s) 277, 593, 637, 800, 939
NcoI CCATGG 1 cut(s) 550
NdeI CATATG 1 cut(s) 781
NdeII GATC 5 cut(s) 130, 186, 613, 762, 1156
NlaIII CATG 9 cut(s) 260, 527, 549, 554, 683, 714, 820, 1044, 1131
NlaIV GGNNCC 2 cut(s) 376, 876
NmuCI GTSAC 1 cut(s) 161
NspI RCATGY 3 cut(s) 527, 683, 820
PauI GCGCGC 1 cut(s) 583
PctI GAATGC 1 cut(s) 891
PdmI GAANNNNTTC 1 cut(s) 327
PfeI GAWTC 6 cut(s) 79, 100, 224, 686, 922, 956
PflMI CCANNNNNTGG 3 cut(s) 367, 463, 551
PkrI GCNGC 7 cut(s) 127, 273, 276, 279, 374, 909, 912
PleI GAGTC 1 cut(s) 1059
PpsI GAGTC 1 cut(s) 1059
Psp124BI GAGCTC 1 cut(s) 1096
PspN4I GGNNCC 2 cut(s) 376, 876
PspPI GGNCC 5 cut(s) 4, 350, 554, 707, 874
PsuI RGATCY 1 cut(s) 613
PteI GCGCGC 1 cut(s) 583
PvuII CAGCTG 1 cut(s) 910
RsaI GTAC 4 cut(s) 96, 451, 743, 1173
RsaNI GTAC 4 cut(s) 95, 450, 742, 1172
RseI CAYNNNNRTG 2 cut(s) 815, 821
SacI GAGCTC 1 cut(s) 1096
SalI GTCGAC 1 cut(s) 426
SaqAI TTAA 5 cut(s) 476, 519, 731, 980, 1011
SatI GCNGC 7 cut(s) 126, 272, 275, 278, 373, 908, 911
Sau3AI GATC 5 cut(s) 130, 186, 613, 762, 1156
Sau96I GGNCC 5 cut(s) 4, 350, 554, 707, 874
SchI GAGTC 1 cut(s) 1060
SduI GDGCHC 1 cut(s) 1096
SfaNI GCATC 5 cut(s) 196, 324, 781, 790, 819
SfcI CTRYAG 1 cut(s) 690
SinI GGWCC 4 cut(s) 4, 350, 707, 874
SmiMI CAYNNNNRTG 2 cut(s) 815, 821
Sse9I AATT 8 cut(s) 292, 397, 418, 477, 511, 785, 977, 1058
SsiI CCGC 5 cut(s) 271, 274, 277, 372, 794
SspMI CTAG 2 cut(s) 66, 1222
SstI GAGCTC 1 cut(s) 1096
StyI CCWWGG 1 cut(s) 550
TaaI ACNGT 2 cut(s) 489, 998
TaiI ACGT 2 cut(s) 747, 775
TaqI TCGA 3 cut(s) 143, 427, 769
TasI AATT 8 cut(s) 292, 397, 418, 477, 511, 785, 977, 1058
TatI WGTACW 2 cut(s) 94, 1171
TauI GCSGC 4 cut(s) 274, 277, 280, 375
TfiI GAWTC 6 cut(s) 79, 100, 224, 686, 922, 956
Tru1I TTAA 5 cut(s) 476, 519, 731, 980, 1011
Tru9I TTAA 5 cut(s) 476, 519, 731, 980, 1011
TseFI GTSAC 1 cut(s) 161
TseI GCWGC 3 cut(s) 125, 907, 910
Tsp45I GTSAC 1 cut(s) 161
TspDTI ATGAA 4 cut(s) 245, 798, 1116, 1135
TspGWI ACGGA 2 cut(s) 646, 887
Van91I CCANNNNNTGG 3 cut(s) 367, 463, 551
VpaK11BI GGWCC 4 cut(s) 4, 350, 707, 874
XapI RAATTY 2 cut(s) 418, 1058
XceI RCATGY 3 cut(s) 527, 683, 820
XmiI GTMKAC 1 cut(s) 427
XmnI GAANNNNTTC 1 cut(s) 327
XspI CTAG 2 cut(s) 66, 1222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.