Rroxscaffold_1G00001100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000990
Physical Location & Seq
Forward (+)
1544524 .. 1558805
14282 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00001100.1

Sequence Viewer

Length: 3234 bp
ATGGCTAGATGGGATGAGATTCTGTCTCTTCCTGTACAGAATCCTCCGACATTGGAGTTTTCTGCTGCTGATCTTGTGTGGTCGAAGGTGGAAGGTTGGCGTGACAAGAAAGATAGAGTTGCTGTAATCCCCTATGCCAGAGTTGATGCTTTTGTGAGGGGTGAATCTTCGAACAAAGAGTGTCTGACGAGATTTCATGTTGAAGCGAGGCGGCGGCGGCAGGCAAAGACGGATTGCAAACCAAAGGTTGATGGCATCCTTGAATATATTCTATATTGGTGTTCCTTTGGTCCTGATGACCACAGGATGGGTGGCTCTATACGACCCAGTAGGACTAATTATGTCCCAAAGAAGAAAAATTCTGGTCGACCAAACACCAAGAGAGGTTGTACCTGCCACTTTATGGTGAAACGCTTAATTGCAGAACCGTCAGTAGCCCTTCTTGTATATAATTCTGTTAAGCATGTGGATAAGAAAGGACTGCCATGCCATGGGCCAGAAGATAAGATGGCTGCTGGAACGCGCGCTATGTTTGCCCCATACATCTCTGAGGATCTACGCCTTCGTGTGCTATCTTTGCTATATGTGGGGGTCTCCGTGGAGACCATAATGCAGAGACACACTGAATCCGTAGAGAGACATGGTGGTCCATGTAATCGTGATGACCTCTTAACCCATAGGTACGTTCGCAGACAGGAGAGGATCATTCGACGTTCTACATATGAATTGGATGAGGATGATGCAGTTAGCATCAGCATGTGGGTGGAAAACCATCAGAATAGCATTTTCTTCTATGAGGATTTCTCCGAGACGGACCCTTTCACTTTGGGCATTCAAACAGACTGGCAGCTGCAACAGATGATTCGCTTTGGCAATCACAGCCTTATAGCCTCTGATTCAAGGTTCGGAACAAACAAATTAAAGTATCCTGTTTGCAGTCTCCTTGTATTTAACCAAGACAACAAGGCCATTCCGGTGGCATGGATAGTAAGTCCAAAATTTGCAAGTTCTGATACCCATAAATGGATGAGAGCTCTTTACAATAGAGTTCAGACAAAAGATCCTTCATGGAAGTTAGCTGGGTTCATAGTGGATGATCCGCAGGCTGATGTACACACTATCAGGGATGTATTTCAGTGCTCAGTGCTGATAAGCTTCTGGCGGGTTCGTCATGCATGGCATAAAAACTTGGTAAAGAAATGTTTGGACGACGAGATGCGAGCTACAATATCAAGAAGGCTTCATCAGGCAGTAGATGACATATGTCAACAACGAGGAACAGATGGTTCGTTTGCGGATTTCATGGAAGATTTTGTTGATGCATCAGATTTTGTGGATTACTTTAAGGCAACCTGGTATCCTAGAATAGGGAAGTGGACTACTGCATTAGAAAGTCTTCCTCTCGCTAGCCAGGAAACATGTGCAGCAATGGAATTCTACCACAACCAACTCAAGCTTAGGTTATTGAATGAGAAGAAACCCAGTGTCTGCAAAAGAGTTGATTGGCTGGTTGATAAGCTGGGTACCCAAGTGCATTCCTATTTCTGGCTTGATGAATACTCTGAGAAGGATGATTTTGCACGATACTGGAAAGATGAGTGGGTGAACGGTTTAACATCTTGGCGAACAGCTTTGGAGATTCCAGACTCTAATGTTGTCATTGAAGGTACACGTGCAAAAGTAACAGACCAGCTTGATCAAGAAAAGGCTTTTGTTGTTTGGAACCCTGGTTCACAATTCAGTATTTGCAACTGCAGTTGGGCAGAAATGGGGAACCTGTGTGAGCATATACTCAAGGTTATTAGTGTCTGCAGGAAAAGAAGTCATCCTGTGCCATCTGTCAGCTTATTGCAGTTCCATCAGGCCTTGCTTGATATGCTCCATTGCCCACCTCATGATTCTTTAATTCGTGATCACGCTGTTTCTTTAGCGGTCTTTGTACAGAACCAGTTAAGTGGACCTAATTTGGAAGGCAGCAATAGCACAATGGATCAGGCAGCTTTAGCCGATAAAGATCTAGAAATAGTAAATGAGGGCCCAGTAAGTGAAGAAGTATTATCTCATAATGAGAATAGTTGTGCGGATGACCATGTTGCTGTGGGTGCTGAAACTAGTAGTCCAGTTGCATGTGGAAATGACATATATAATGAGGGTTGTGGAGAAGAAATCACTTCTTCTGAGATGGATGTTGATCCATCATCCATCTGTATTTCTCCACCTGGGTTACATTCTGTTGATGAGGTTGTGTCTAGTGGTGTCCTCTCAGGAAGCAGACAGAGGCCCTTGTTTAACACAGAAACTGAAGATCTGCTTTCCACAGATGATCCTCTCACTAATCCAAGTGAATATGAGGATGATATTTTAGATGAAAATTGCGAAAAAAATGTGATGAGTATGGATCCACTCTCCTTTCATATCCCTTGTTTAACAATGGAGTTTGTGGAGCAATGCACGGTACCCCATCCTAATGATCTCCACAGCCATGAAGTCAATCCCACCATTATTTCGAAAGTATGTGATGACAGTACAGTTAACAACAATACCTCCTCATCTTCATCTGTGCCTCTTGAACCACAAGCTTCAGGTGTCATAGAAAAAGAGAACATGGAAATTGAAAGTATGGATGGAATGGACTTTGTGGAGCATTGCGCATTAACCAATCCCAACCGTAACCACAGCCGTGATATCGATATTGAGCCTACTGTTATTTCCAAAAGATCTCCGGACAATACTGTTTTTAACAAGACCTCTGCATCTTCATCTATGCCTGTTGAATCAGAAATGGTTGAAGTGGCTGAAACTTCAGGTTTCATTTTAGAAAATGATAGAATGGAAATTGGAAGTGAGAATGGAAGCACAAGTAAGCACAATCTGTCTGCTAATAATCCTTCCACCTTGGACGGCGTTCGTGGTTGTATGATAAATGGTTCAGATTGTAGTCAGGATTCTGAGTCAGTTGGCAATGCAATGGTTGTGTACTCAGAACCATTGCTCAAGTTCTCATCAACATCAAGTTGCAACAAGGAAGAGCTGCCTGTTGAGAATAGGGCTACTGAAAGTGGGTCTGGTGAGAAGCCATCTTCAATCACTGAATCTCATCTTAAAAAGTGTAGACTTCGAGAGGGTGCCAAAATCATCAACAACAATGAGGTGATCCACCAGTCAGTTATCAGTGACATCAATGTTACTTCTGTAAAGGGAAATGCTCGAGGTGAATCTGCTAATGCCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1077

Amino Acids

121.05

Weight (kDa)

5.23

Isoelectric Point (pI)

43.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 645
Acc16I TGCGCA 1 cut(s) 2650
Acc36I ACCTGC 1 cut(s) 401
Acc65I GGTACC 2 cut(s) 1523, 2455
AccB1I GGYRCC 3 cut(s) 1523, 2455, 3125
AccB7I CCANNNNNTGG 3 cut(s) 307, 403, 491
AccI GTMKAC 2 cut(s) 367, 3112
AccII CGCG 2 cut(s) 523, 525
AccIII TCCGGA 1 cut(s) 2722
AciI CCGC 8 cut(s) 211, 214, 217, 1100, 1162, 1295, 1931, 2081
AcsI RAATTY 3 cut(s) 358, 998, 1433
AcuI CTGAAG 3 cut(s) 2322, 2565, 2787
AcvI CACGTG 1 cut(s) 1673
AfiI CCNNNNNNNGG 6 cut(s) 307, 403, 491, 1023, 1367, 1545
AflIII ACRYGT 2 cut(s) 1418, 1670
AhlI ACTAGT 1 cut(s) 2111
AjnI CCWGG 4 cut(s) 1352, 1410, 1726, 2218
AleI CACNNNNGTG 1 cut(s) 2679
AloI GAACNNNNNNTCC 2 cut(s) 1714, 1746
Alw21I GWGCWC 2 cut(s) 1036, 1142
Alw26I GTCTC 7 cut(s) 30, 596, 598, 610, 631, 803, 944
AlwNI CAGNNNCTG 2 cut(s) 1488, 2300
Ama87I CYCGRG 1 cut(s) 3207
Aor13HI TCCGGA 1 cut(s) 2722
AoxI GGCC 5 cut(s) 494, 966, 1863, 2035, 2279
ApaI GGGCCC 1 cut(s) 2039
ApeKI GCWGC 8 cut(s) 65, 512, 847, 850, 1424, 1974, 1997, 3031
ApoI RAATTY 3 cut(s) 358, 998, 1433
Asp700I GAANNNNTTC 2 cut(s) 267, 1395
Asp718I GGTACC 2 cut(s) 1523, 2455
AspLEI GCGC 3 cut(s) 525, 527, 2651
AspS9I GGNCC 8 cut(s) 290, 494, 647, 814, 1958, 2035, 2036, 2280
AsuHPI GGTGA 6 cut(s) 173, 418, 1615, 3080, 3163, 3224
AsuII TTCGAA 2 cut(s) 170, 2507
AsuNHI GCTAGC 1 cut(s) 1406
AvaI CYCGRG 1 cut(s) 3207
AvaII GGWCC 4 cut(s) 290, 647, 814, 1958
BaeGI GKGCMC 1 cut(s) 2039
BaeI ACNNNNGTAYC 2 cut(s) 1515, 1548
BamHI GGATCC 1 cut(s) 2398
BanI GGYRCC 3 cut(s) 1523, 2455, 3125
BanII GRGCYC 2 cut(s) 1036, 2039
BbrPI CACGTG 1 cut(s) 1673
BbsI GAAGAC 1 cut(s) 1388
Bbv12I GWGCWC 2 cut(s) 1036, 1142
BbvI GCAGC 8 cut(s) 52, 499, 837, 859, 1436, 1986, 2009, 3018
BceAI ACGGC 2 cut(s) 2664, 2917
BciT130I CCWGG 4 cut(s) 1354, 1412, 1728, 2220
BciVI GTATCC 2 cut(s) 936, 1368
BclI TGATCA 2 cut(s) 1696, 1912
BcoDI GTCTC 7 cut(s) 30, 596, 598, 610, 631, 803, 944
BcuI ACTAGT 1 cut(s) 2111
BfaI CTAG 6 cut(s) 6, 1362, 1407, 2018, 2112, 2250
BfmI CTRYAG 2 cut(s) 1753, 1810
BfuAI ACCTGC 1 cut(s) 401
BfuI GTATCC 2 cut(s) 936, 1368
BglII AGATCT 3 cut(s) 2014, 2305, 2717
Bme1390I CCNGG 4 cut(s) 1354, 1412, 1728, 2220
Bme18I GGWCC 4 cut(s) 290, 647, 814, 1958
BmeT110I CYCGRG 1 cut(s) 3207
BmgT120I GGNCC 8 cut(s) 290, 494, 647, 814, 1958, 2035, 2036, 2280
BmiI GGNNCC 8 cut(s) 816, 1525, 1724, 1775, 2037, 2400, 2457, 3127
BmrFI CCNGG 4 cut(s) 1354, 1412, 1728, 2220
BmrI ACTGGG 3 cut(s) 321, 1476, 2033
BmsI GCATC 8 cut(s) 136, 264, 730, 759, 1206, 1309, 1331, 2762
BmtI GCTAGC 1 cut(s) 1410
BmuI ACTGGG 3 cut(s) 321, 1476, 2033
BoxI GACNNNNGTC 1 cut(s) 1263
BpiI GAAGAC 1 cut(s) 1388
BplI GAGNNNNNCTC 2 cut(s) 788, 820
Bpu10I CCTNAGC 1 cut(s) 1457
Bpu14I TTCGAA 2 cut(s) 170, 2507
BpuEI CTTGAG 3 cut(s) 1436, 1778, 2978
Bsa29I ATCGAT 1 cut(s) 2688
BsaAI YACGTR 1 cut(s) 1673
BsaBI GATNNNNATC 2 cut(s) 2013, 2190
BsaI GGTCTC 2 cut(s) 596, 598
BsaJI CCNNGG 5 cut(s) 490, 597, 1726, 2219, 2895
BsaWI WCCGGW 2 cut(s) 973, 2722
BsaXI ACNNNNNCTCC 2 cut(s) 2528, 2558
Bsc4I CCNNNNNNNGG 6 cut(s) 307, 403, 491, 1023, 1367, 1545
Bse1I ACTGG 8 cut(s) 327, 848, 1482, 1592, 1948, 2039, 2120, 3160
Bse3DI GCAATG 7 cut(s) 1434, 1882, 2453, 2644, 2968, 2973, 2987
Bse8I GATNNNNATC 2 cut(s) 2013, 2190
BseAI TCCGGA 1 cut(s) 2722
BseBI CCWGG 4 cut(s) 1354, 1412, 1728, 2220
BseCI ATCGAT 1 cut(s) 2688
BseDI CCNNGG 5 cut(s) 490, 597, 1726, 2219, 2895
BseJI GATNNNNATC 2 cut(s) 2013, 2190
BseLI CCNNNNNNNGG 6 cut(s) 307, 403, 491, 1023, 1367, 1545
BseMI GCAATG 7 cut(s) 1434, 1882, 2453, 2644, 2968, 2973, 2987
BseMII CTCAG 7 cut(s) 540, 1155, 1554, 2169, 2277, 2940, 2994
BseNI ACTGG 8 cut(s) 327, 848, 1482, 1592, 1948, 2039, 2120, 3160
BsePI GCGCGC 1 cut(s) 523
BseRI GAGGAG 1 cut(s) 2536
BseSI GKGCMC 1 cut(s) 2039
BseXI GCAGC 8 cut(s) 52, 499, 837, 859, 1436, 1986, 2009, 3018
BseYI CCCAGC 2 cut(s) 1079, 1519
BsgI GTGCAG 1 cut(s) 1443
Bsh1236I CGCG 2 cut(s) 523, 525
BshFI GGCC 5 cut(s) 496, 968, 1865, 2037, 2281
BshNI GGYRCC 3 cut(s) 1523, 2455, 3125
BshVI ATCGAT 1 cut(s) 2688
BsiHKAI GWGCWC 2 cut(s) 1036, 1142
BsiHKCI CYCGRG 1 cut(s) 3207
BsiSI CCGG 2 cut(s) 974, 2723
BslFI GGGAC 1 cut(s) 329
BslI CCNNNNNNNGG 6 cut(s) 307, 403, 491, 1023, 1367, 1545
BsmAI GTCTC 7 cut(s) 30, 596, 598, 610, 631, 803, 944
BsmBI CGTCTC 1 cut(s) 803
BsmFI GGGAC 1 cut(s) 329
BsmI GAATGC 2 cut(s) 831, 1534
BsnI GGCC 5 cut(s) 496, 968, 1865, 2037, 2281
Bso31I GGTCTC 2 cut(s) 596, 598
BsoBI CYCGRG 1 cut(s) 3207
Bsp119I TTCGAA 2 cut(s) 170, 2507
Bsp120I GGGCCC 1 cut(s) 2035
Bsp1286I GDGCHC 3 cut(s) 1036, 1142, 2039
Bsp13I TCCGGA 1 cut(s) 2722
Bsp1407I TGTACA 3 cut(s) 34, 1111, 1939
Bsp19I CCATGG 1 cut(s) 490
BspACI CCGC 8 cut(s) 211, 214, 217, 1100, 1162, 1295, 1931, 2081
BspANI GGCC 5 cut(s) 496, 968, 1865, 2037, 2281
BspCNI CTCAG 7 cut(s) 541, 1154, 1555, 2170, 2276, 2941, 2993
BspDI ATCGAT 1 cut(s) 2688
BspEI TCCGGA 1 cut(s) 2722
BspFNI CGCG 2 cut(s) 523, 525
BspHI TCATGA 1 cut(s) 1894
BspLI GGNNCC 8 cut(s) 816, 1525, 1724, 1775, 2037, 2400, 2457, 3127
BspMAI CTGCAG 2 cut(s) 1757, 1814
BspMI ACCTGC 1 cut(s) 401
BspOI GCTAGC 1 cut(s) 1410
BspQI GCTCTTC 1 cut(s) 3021
BspT104I TTCGAA 2 cut(s) 170, 2507
BspT107I GGYRCC 3 cut(s) 1523, 2455, 3125
BspTNI GGTCTC 2 cut(s) 596, 598
BsrDI GCAATG 7 cut(s) 1434, 1882, 2453, 2644, 2968, 2973, 2987
BsrGI TGTACA 3 cut(s) 34, 1111, 1939
BsrI ACTGG 8 cut(s) 327, 848, 1482, 1592, 1948, 2039, 2120, 3160
BssECI CCNNGG 5 cut(s) 490, 597, 1726, 2219, 2895
BssHII GCGCGC 1 cut(s) 523
BssT1I CCWWGG 2 cut(s) 490, 2895
Bst2UI CCWGG 4 cut(s) 1354, 1412, 1728, 2220
Bst4CI ACNGT 8 cut(s) 429, 1610, 2455, 2525, 2530, 2669, 2704, 2734
Bst6I CTCTTC 2 cut(s) 33, 3021
BstAUI TGTACA 3 cut(s) 34, 1111, 1939
BstBAI YACGTR 1 cut(s) 1673
BstBI TTCGAA 2 cut(s) 170, 2507
BstC8I GCNNGC 5 cut(s) 222, 525, 1104, 1221, 1408
BstDEI CTNAG 8 cut(s) 549, 1141, 1457, 1563, 2178, 2263, 2949, 2980
BstDSI CCRYGG 2 cut(s) 490, 597
BstENI CCTNNNNNAGG 1 cut(s) 1365
BstFNI CGCG 2 cut(s) 523, 525
BstHHI GCGC 3 cut(s) 525, 527, 2651
BstMAI GTCTC 7 cut(s) 30, 596, 598, 610, 631, 803, 944
BstMWI GCNNNNNNNGC 8 cut(s) 217, 533, 577, 879, 1876, 1980, 2003, 2102
BstNI CCWGG 4 cut(s) 1354, 1412, 1728, 2220
BstNSI RCATGY 4 cut(s) 467, 760, 1422, 2130
BstPAI GACNNNNGTC 1 cut(s) 1263
BstSCI CCNGG 4 cut(s) 1352, 1410, 1726, 2218
BstSFI CTRYAG 2 cut(s) 1753, 1810
BstSLI GKGCMC 1 cut(s) 2039
BstUI CGCG 2 cut(s) 523, 525
BstV1I GCAGC 8 cut(s) 52, 499, 837, 859, 1436, 1986, 2009, 3018
BstV2I GAAGAC 1 cut(s) 1388
BstX2I RGATCY 6 cut(s) 553, 1060, 2014, 2305, 2398, 2717
BstXI CCANNNNNNTGG 2 cut(s) 976, 1955
BstYI RGATCY 6 cut(s) 553, 1060, 2014, 2305, 2398, 2717
Bsu15I ATCGAT 1 cut(s) 2688
BsuI GTATCC 2 cut(s) 936, 1368
BsuRI GGCC 5 cut(s) 496, 968, 1865, 2037, 2281
BsuTUI ATCGAT 1 cut(s) 2688
BtgI CCRYGG 2 cut(s) 490, 597
BtsIMutI CAGTG 6 cut(s) 621, 1142, 1149, 1489, 3087, 3178
BveI ACCTGC 1 cut(s) 401
Cac8I GCNNGC 5 cut(s) 222, 525, 1104, 1221, 1408
CaiI CAGNNNCTG 2 cut(s) 1488, 2300
CciI TCATGA 1 cut(s) 1894
CfoI GCGC 3 cut(s) 525, 527, 2651
Cfr13I GGNCC 8 cut(s) 290, 494, 647, 814, 1958, 2035, 2036, 2280
ClaI ATCGAT 1 cut(s) 2688
CsiI ACCWGGT 1 cut(s) 1352
DdeI CTNAG 8 cut(s) 549, 1141, 1457, 1563, 2178, 2263, 2949, 2980
DrdI GACNNNNNNGTC 1 cut(s) 645
DseDI GACNNNNNNGTC 1 cut(s) 645
Eam1104I CTCTTC 2 cut(s) 33, 3021
EarI CTCTTC 2 cut(s) 33, 3021
Ecl136II GAGCTC 1 cut(s) 1034
Eco130I CCWWGG 2 cut(s) 490, 2895
Eco147I AGGCCT 1 cut(s) 1865
Eco24I GRGCYC 2 cut(s) 1036, 2039
Eco31I GGTCTC 2 cut(s) 596, 598
Eco32I GATATC 1 cut(s) 2686
Eco47I GGWCC 4 cut(s) 290, 647, 814, 1958
Eco53kI GAGCTC 1 cut(s) 1034
Eco57I CTGAAG 3 cut(s) 2322, 2565, 2787
Eco72I CACGTG 1 cut(s) 1673
Eco88I CYCGRG 1 cut(s) 3207
EcoICRI GAGCTC 1 cut(s) 1034
EcoNI CCTNNNNNAGG 1 cut(s) 1365
EcoO109I RGGNCCY 2 cut(s) 2035, 2280
EcoRI GAATTC 1 cut(s) 1433
EcoRII CCWGG 4 cut(s) 1352, 1410, 1726, 2218
EcoRV GATATC 1 cut(s) 2686
EcoT14I CCWWGG 2 cut(s) 490, 2895
EcoT22I ATGCAT 2 cut(s) 1177, 1324
EcoT38I GRGCYC 2 cut(s) 1036, 2039
ErhI CCWWGG 2 cut(s) 490, 2895
Esp3I CGTCTC 1 cut(s) 803
FalI AAGNNNNNCTT 2 cut(s) 2295, 2327
FaqI GGGAC 1 cut(s) 329
FauI CCCGC 1 cut(s) 1155
FauNDI CATATG 2 cut(s) 721, 1262
FbaI TGATCA 2 cut(s) 1696, 1912
FblI GTMKAC 2 cut(s) 367, 3112
FriOI GRGCYC 2 cut(s) 1036, 2039
FspBI CTAG 6 cut(s) 6, 1362, 1407, 2018, 2112, 2250
FspI TGCGCA 1 cut(s) 2650
GlaI GCGC 3 cut(s) 524, 526, 2650
GsaI CCCAGC 2 cut(s) 1083, 1523
HaeIII GGCC 5 cut(s) 496, 968, 1865, 2037, 2281
HapII CCGG 2 cut(s) 974, 2723
HhaI GCGC 3 cut(s) 525, 527, 2651
Hin6I GCGC 3 cut(s) 523, 525, 2649
HinP1I GCGC 3 cut(s) 523, 525, 2649
HincII GTYRAC 3 cut(s) 368, 1268, 2533
HindII GTYRAC 3 cut(s) 368, 1268, 2533
HindIII AAGCTT 3 cut(s) 1153, 1454, 2577
HpaI GTTAAC 1 cut(s) 2533
HpaII CCGG 2 cut(s) 974, 2723
HphI GGTGA 6 cut(s) 173, 418, 1615, 3080, 3163, 3224
Hpy99I CGWCG 2 cut(s) 714, 1214
HpyCH4III ACNGT 8 cut(s) 429, 1610, 2455, 2525, 2530, 2669, 2704, 2734
HpyCH4IV ACGT 3 cut(s) 684, 712, 1672
HpyF10VI GCNNNNNNNGC 8 cut(s) 217, 533, 577, 879, 1876, 1980, 2003, 2102
HpyF3I CTNAG 8 cut(s) 549, 1141, 1457, 1563, 2178, 2263, 2949, 2980
HpySE526I ACGT 3 cut(s) 684, 712, 1672
HspAI GCGC 3 cut(s) 523, 525, 2649
Kpn2I TCCGGA 1 cut(s) 2722
KpnI GGTACC 2 cut(s) 1527, 2459
Ksp22I TGATCA 2 cut(s) 1696, 1912
KspAI GTTAAC 1 cut(s) 2533
LguI GCTCTTC 1 cut(s) 3021
LmnI GCTCC 3 cut(s) 1884, 2443, 2641
Lsp1109I GCAGC 8 cut(s) 52, 499, 837, 859, 1436, 1986, 2009, 3018
LweI GCATC 8 cut(s) 136, 264, 730, 759, 1206, 1309, 1331, 2762
MabI ACCWGGT 1 cut(s) 1352
MaeI CTAG 6 cut(s) 6, 1362, 1407, 2018, 2112, 2250
MaeII ACGT 3 cut(s) 684, 712, 1672
MaeIII GTNAC 6 cut(s) 101, 1681, 2223, 2669, 3173, 3184
MflI RGATCY 6 cut(s) 553, 1060, 2014, 2305, 2398, 2717
MhlI GDGCHC 3 cut(s) 1036, 1142, 2039
MlyI GAGTC 2 cut(s) 1640, 2960
MmeI TCCRAC 1 cut(s) 71
Mph1103I ATGCAT 2 cut(s) 1177, 1324
MroI TCCGGA 1 cut(s) 2722
MroXI GAANNNNTTC 2 cut(s) 267, 1395
MslI CAYNNNNRTG 6 cut(s) 755, 761, 974, 2466, 2481, 2679
MspA1I CMGCKG 1 cut(s) 850
MspI CCGG 2 cut(s) 974, 2723
MspR9I CCNGG 4 cut(s) 1354, 1412, 1728, 2220
Mva1269I GAATGC 2 cut(s) 831, 1534
MvaI CCWGG 4 cut(s) 1354, 1412, 1728, 2220
MvnI CGCG 2 cut(s) 523, 525
MwoI GCNNNNNNNGC 8 cut(s) 217, 533, 577, 879, 1876, 1980, 2003, 2102
NcoI CCATGG 1 cut(s) 490
NdeI CATATG 2 cut(s) 721, 1262
NheI GCTAGC 1 cut(s) 1406
NlaIV GGNNCC 8 cut(s) 816, 1525, 1724, 1775, 2037, 2400, 2457, 3127
NmuCI GTSAC 2 cut(s) 101, 3173
NsbI TGCGCA 1 cut(s) 2650
NsiI ATGCAT 2 cut(s) 1177, 1324
NspI RCATGY 4 cut(s) 467, 760, 1422, 2130
NspV TTCGAA 2 cut(s) 170, 2507
OliI CACNNNNGTG 1 cut(s) 2679
PaeR7I CTCGAG 1 cut(s) 3207
PagI TCATGA 1 cut(s) 1894
PauI GCGCGC 1 cut(s) 523
PceI AGGCCT 1 cut(s) 1865
PciI ACATGT 1 cut(s) 1418
PciSI GCTCTTC 1 cut(s) 3021
PctI GAATGC 2 cut(s) 831, 1534
PdmI GAANNNNTTC 2 cut(s) 267, 1395
PflMI CCANNNNNTGG 3 cut(s) 307, 403, 491
PleI GAGTC 2 cut(s) 1640, 2959
PmaCI CACGTG 1 cut(s) 1673
PmlI CACGTG 1 cut(s) 1673
PpsI GAGTC 2 cut(s) 1640, 2959
Ppu21I YACGTR 1 cut(s) 1673
PscI ACATGT 1 cut(s) 1418
PshAI GACNNNNGTC 1 cut(s) 1263
Psp124BI GAGCTC 1 cut(s) 1036
Psp6I CCWGG 4 cut(s) 1352, 1410, 1726, 2218
PspCI CACGTG 1 cut(s) 1673
PspFI CCCAGC 2 cut(s) 1079, 1519
PspGI CCWGG 4 cut(s) 1352, 1410, 1726, 2218
PspN4I GGNNCC 8 cut(s) 816, 1525, 1724, 1775, 2037, 2400, 2457, 3127
PspOMI GGGCCC 1 cut(s) 2035
PspPI GGNCC 8 cut(s) 290, 494, 647, 814, 1958, 2035, 2036, 2280
PspXI VCTCGAGB 1 cut(s) 3207
PstI CTGCAG 2 cut(s) 1757, 1814
PstNI CAGNNNCTG 2 cut(s) 1488, 2300
PsuI RGATCY 6 cut(s) 553, 1060, 2014, 2305, 2398, 2717
PteI GCGCGC 1 cut(s) 523
PvuII CAGCTG 1 cut(s) 850
RseI CAYNNNNRTG 6 cut(s) 755, 761, 974, 2466, 2481, 2679
SacI GAGCTC 1 cut(s) 1036
SalI GTCGAC 1 cut(s) 366
SapI GCTCTTC 1 cut(s) 3021
Sau96I GGNCC 8 cut(s) 290, 494, 647, 814, 1958, 2035, 2036, 2280
SchI GAGTC 2 cut(s) 1640, 2960
ScrFI CCNGG 4 cut(s) 1354, 1412, 1728, 2220
SduI GDGCHC 3 cut(s) 1036, 1142, 2039
SexAI ACCWGGT 1 cut(s) 1352
SfaNI GCATC 8 cut(s) 136, 264, 730, 759, 1206, 1309, 1331, 2762
SfcI CTRYAG 2 cut(s) 1753, 1810
Sfr274I CTCGAG 1 cut(s) 3207
SfuI TTCGAA 2 cut(s) 170, 2507
SinI GGWCC 4 cut(s) 290, 647, 814, 1958
SlaI CTCGAG 1 cut(s) 3207
SmiMI CAYNNNNRTG 6 cut(s) 755, 761, 974, 2466, 2481, 2679
SmlI CTYRAG 4 cut(s) 1451, 1793, 2993, 3207
SmoI CTYRAG 4 cut(s) 1451, 1793, 2993, 3207
SpeI ACTAGT 1 cut(s) 2111
SseBI AGGCCT 1 cut(s) 1865
SsiI CCGC 8 cut(s) 211, 214, 217, 1100, 1162, 1295, 1931, 2081
SspMI CTAG 6 cut(s) 6, 1362, 1407, 2018, 2112, 2250
SstI GAGCTC 1 cut(s) 1036
StuI AGGCCT 1 cut(s) 1865
StyD4I CCNGG 4 cut(s) 1352, 1410, 1726, 2218
StyI CCWWGG 2 cut(s) 490, 2895
TaaI ACNGT 8 cut(s) 429, 1610, 2455, 2525, 2530, 2669, 2704, 2734
TaiI ACGT 3 cut(s) 687, 715, 1675
TaqI TCGA 8 cut(s) 83, 170, 367, 709, 2507, 2688, 3118, 3208
TatI WGTACW 5 cut(s) 34, 1111, 1939, 2525, 2976
TauI GCSGC 3 cut(s) 214, 217, 220
TscAI CASTG 6 cut(s) 628, 1142, 1149, 1489, 3094, 3178
TseFI GTSAC 2 cut(s) 101, 3173
TseI GCWGC 8 cut(s) 65, 512, 847, 850, 1424, 1974, 1997, 3031
Tsp45I GTSAC 2 cut(s) 101, 3173
TspGWI ACGGA 4 cut(s) 245, 586, 619, 827
TspRI CASTG 6 cut(s) 628, 1142, 1149, 1489, 3094, 3178
Van91I CCANNNNNTGG 3 cut(s) 307, 403, 491
VpaK11BI GGWCC 4 cut(s) 290, 647, 814, 1958
XagI CCTNNNNNAGG 1 cut(s) 1365
XapI RAATTY 3 cut(s) 358, 998, 1433
XbaI TCTAGA 1 cut(s) 2017
XceI RCATGY 4 cut(s) 467, 760, 1422, 2130
XcmI CCANNNNNNNNNTGG 1 cut(s) 308
XhoI CTCGAG 1 cut(s) 3207
XmiI GTMKAC 2 cut(s) 367, 3112
XmnI GAANNNNTTC 2 cut(s) 267, 1395
XspI CTAG 6 cut(s) 6, 1362, 1407, 2018, 2112, 2250
Zsp2I ATGCAT 2 cut(s) 1177, 1324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.