Rh5AG538000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000990
Physical Location & Seq
Reverse (-)
92562962 .. 92566595
3634 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG538000.1

Sequence Viewer

Length: 1281 bp
ATGGTTGCCAATTGGTCTAGTCCAGTTTCGGCAGCGCCGGTTTCCCTCCCTATTTCGCCTTCTCTTTTGCAGATGGCTAGATGGGATGAGATTCTGTCTCTTCCTGTACAGAATCCTCCGGCTTTGGAGTTTTCTGCTGCTGATCTTGTGTGGTCGAAGGTGGAAGGTTGGCGTGACAAGAAAGATAGAGTTGCTGTGATCCCCTATGCCAGAGTTGATGCTTTTGTGAGGGGTGAATCTTCGAACAAAGAGTGTCCGACGAGATTTCATGTTGAAGCGAGGCGGCGGCGGCAGGCAAAGACGAATTGCAAACCAAAGGTTGATGGCATCCTTGAATATATTCTATATTGGTGTTCCTTTGGTCCTGATGACCACAGGATGGGCGGCTCCACACGACCCAGTAGGACTAATTATGTCCCAAAGAAGAAAAATTCTGGTCGACCAAACACCAAGAGAGGTTGTACCTGCCACTTTATGGTGAAACGCTTAATTGCTGAACCGTCAGTAGCGCTTCTTGTATATAATTCTGTTAAGCATGTGGATAAGAAAGGGTTGCCATGCCATGGCCCTGAAGATAAGATGGCTTCTGGAACGCGCGCTATGTTTGCCCCATACATCTCTGAGGATCTACGCCTTCGTGTGCTATCTTTGCTATATGTGGGGGTCTCCGTGGAGACCATAATGCAGAGACATGCTGAATCTGTAGAGAGACAGGGTGGTCCATGTAATCGTGATGACCTCTTAACCCATAGGTACGTTCGCAGACAGGAGAGGATCATTCGACGTTCTACATATGAATTGGATGCGGATGATGCAGTTAGCATCGGCATGTGGGTGGAAAACCATCAGAATATCATTTTCTTCTATGAGGATTTCTCCGAGACGGACCCTTTCACTTTGGGCATTCAAACAGACTGGCAGCTGCAACAGATGATTCGCTTTGGCAATCACAGCCTTATAGCCTCTGATTCAAGGTTCGGAACAAACAAATTAAAGTATCCTGTTCACAGTCTCCTTGTATTTAACCAAGACAACAAGGCCATTCCGGTAGCATGGATAGTGAGTCCAAAATTTGCAAGTTCTGATACCCATAAATGGATGAGAGCTCTTTACAATAGAGTTCAGACGAAAGATCCTTCATGGAAGTTGGCTGGCTTCATAGTGGATGATCCTCAGGCTGATGGAAGTGGACTACTGCATTACAAAGTCTTCCTCTCACTAGCCAGGAAACATGTGCAGCAATGGAATTCTACCACAACCAACTCAAGCTTAGGTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

426

Amino Acids

48.57

Weight (kDa)

9.41

Isoelectric Point (pI)

48.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 717
Acc36I ACCTGC 1 cut(s) 473
AccB7I CCANNNNNTGG 3 cut(s) 379, 475, 563
AccI GTMKAC 1 cut(s) 439
AccII CGCG 2 cut(s) 595, 597
AciI CCGC 5 cut(s) 283, 286, 289, 384, 806
AclWI GGATC 5 cut(s) 193, 633, 782, 1127, 1163
AcsI RAATTY 3 cut(s) 430, 1070, 1246
AcuI CTGAAG 1 cut(s) 591
AfaI GTAC 3 cut(s) 108, 463, 755
AfeI AGCGCT 1 cut(s) 510
AfiI CCNNNNNNNGG 4 cut(s) 379, 475, 563, 1095
AflIII ACRYGT 1 cut(s) 1231
AgsI TTSAA 4 cut(s) 275, 335, 908, 972
AjnI CCWGG 1 cut(s) 1223
AluBI AGCT 3 cut(s) 922, 1106, 1269
AluI AGCT 3 cut(s) 922, 1106, 1269
Alw21I GWGCWC 1 cut(s) 1108
Alw26I GTCTC 7 cut(s) 102, 668, 670, 682, 703, 875, 1016
AlwI GGATC 5 cut(s) 193, 633, 782, 1127, 1163
Aor51HI AGCGCT 1 cut(s) 510
AoxI GGCC 2 cut(s) 565, 1038
ApeKI GCWGC 5 cut(s) 32, 137, 919, 922, 1237
ApoI RAATTY 3 cut(s) 430, 1070, 1246
Asp700I GAANNNNTTC 1 cut(s) 339
AspLEI GCGC 4 cut(s) 37, 511, 597, 599
AspS9I GGNCC 4 cut(s) 362, 566, 719, 886
AsuHPI GGTGA 2 cut(s) 245, 490
AsuII TTCGAA 1 cut(s) 242
AvaII GGWCC 3 cut(s) 362, 719, 886
AxyI CCTNAGG 1 cut(s) 1173
BanII GRGCYC 1 cut(s) 1108
BbsI GAAGAC 1 cut(s) 1201
Bbv12I GWGCWC 1 cut(s) 1108
BbvI GCAGC 5 cut(s) 44, 124, 909, 931, 1249
BccI CCATC 7 cut(s) 67, 75, 317, 373, 574, 852, 1175
BciT130I CCWGG 1 cut(s) 1225
BciVI GTATCC 1 cut(s) 1008
BcoDI GTCTC 7 cut(s) 102, 668, 670, 682, 703, 875, 1016
BfaI CTAG 3 cut(s) 18, 78, 1220
BfmI CTRYAG 1 cut(s) 702
BfoI RGCGCY 2 cut(s) 38, 512
BfuAI ACCTGC 1 cut(s) 473
BfuI GTATCC 1 cut(s) 1008
BisI GCNGC 9 cut(s) 33, 138, 284, 287, 290, 385, 920, 923, 1238
BlsI GCNGC 9 cut(s) 34, 139, 285, 288, 291, 386, 921, 924, 1239
Bme1390I CCNGG 1 cut(s) 1225
Bme18I GGWCC 3 cut(s) 362, 719, 886
BmgT120I GGNCC 4 cut(s) 362, 566, 719, 886
BmiI GGNNCC 2 cut(s) 388, 888
BmrFI CCNGG 1 cut(s) 1225
BmrI ACTGGG 1 cut(s) 393
BmsI GCATC 5 cut(s) 208, 336, 793, 802, 831
BmuI ACTGGG 1 cut(s) 393
BpiI GAAGAC 1 cut(s) 1201
BplI GAGNNNNNCTC 2 cut(s) 860, 892
Bpu10I CCTNAGC 1 cut(s) 1270
Bpu14I TTCGAA 1 cut(s) 242
BpuEI CTTGAG 1 cut(s) 1249
BsaI GGTCTC 2 cut(s) 668, 670
BsaJI CCNNGG 2 cut(s) 562, 669
BsaWI WCCGGW 1 cut(s) 1045
Bsc4I CCNNNNNNNGG 4 cut(s) 379, 475, 563, 1095
Bse118I RCCGGY 1 cut(s) 37
Bse1I ACTGG 3 cut(s) 23, 399, 920
Bse21I CCTNAGG 1 cut(s) 1173
Bse3DI GCAATG 1 cut(s) 1247
BseBI CCWGG 1 cut(s) 1225
BseDI CCNNGG 2 cut(s) 562, 669
BseGI GGATG 7 cut(s) 91, 327, 384, 808, 814, 1104, 1171
BseLI CCNNNNNNNGG 4 cut(s) 379, 475, 563, 1095
BseMI GCAATG 1 cut(s) 1247
BseMII CTCAG 2 cut(s) 612, 1187
BseNI ACTGG 3 cut(s) 23, 399, 920
BsePI GCGCGC 1 cut(s) 595
BseXI GCAGC 5 cut(s) 44, 124, 909, 931, 1249
BsgI GTGCAG 1 cut(s) 1256
Bsh1236I CGCG 2 cut(s) 595, 597
BshFI GGCC 2 cut(s) 567, 1040
BsiHKAI GWGCWC 1 cut(s) 1108
BsiSI CCGG 3 cut(s) 38, 119, 1046
BslFI GGGAC 1 cut(s) 401
BslI CCNNNNNNNGG 4 cut(s) 379, 475, 563, 1095
BsmAI GTCTC 7 cut(s) 102, 668, 670, 682, 703, 875, 1016
BsmBI CGTCTC 1 cut(s) 875
BsmFI GGGAC 1 cut(s) 401
BsmI GAATGC 1 cut(s) 903
BsnI GGCC 2 cut(s) 567, 1040
Bso31I GGTCTC 2 cut(s) 668, 670
Bsp119I TTCGAA 1 cut(s) 242
Bsp1286I GDGCHC 1 cut(s) 1108
Bsp1407I TGTACA 1 cut(s) 106
Bsp143I GATC 6 cut(s) 142, 198, 625, 774, 1132, 1168
Bsp19I CCATGG 1 cut(s) 562
BspACI CCGC 5 cut(s) 283, 286, 289, 384, 806
BspANI GGCC 2 cut(s) 567, 1040
BspCNI CTCAG 2 cut(s) 613, 1186
BspFNI CGCG 2 cut(s) 595, 597
BspLI GGNNCC 2 cut(s) 388, 888
BspMI ACCTGC 1 cut(s) 473
BspPI GGATC 5 cut(s) 193, 633, 782, 1127, 1163
BspT104I TTCGAA 1 cut(s) 242
BspTNI GGTCTC 2 cut(s) 668, 670
BsrDI GCAATG 1 cut(s) 1247
BsrFI RCCGGY 1 cut(s) 37
BsrGI TGTACA 1 cut(s) 106
BsrI ACTGG 3 cut(s) 23, 399, 920
BssAI RCCGGY 1 cut(s) 37
BssECI CCNNGG 2 cut(s) 562, 669
BssHII GCGCGC 1 cut(s) 595
BssMI GATC 6 cut(s) 142, 198, 625, 774, 1132, 1168
BssT1I CCWWGG 1 cut(s) 562
Bst2UI CCWGG 1 cut(s) 1225
Bst4CI ACNGT 2 cut(s) 501, 1010
Bst6I CTCTTC 1 cut(s) 105
BstAUI TGTACA 1 cut(s) 106
BstBI TTCGAA 1 cut(s) 242
BstC8I GCNNGC 3 cut(s) 294, 597, 1153
BstDEI CTNAG 3 cut(s) 621, 1173, 1270
BstDSI CCRYGG 2 cut(s) 562, 669
BstF5I GGATG 7 cut(s) 91, 327, 384, 808, 814, 1104, 1171
BstFNI CGCG 2 cut(s) 595, 597
BstH2I RGCGCY 2 cut(s) 38, 512
BstHHI GCGC 4 cut(s) 37, 511, 597, 599
BstKTI GATC 6 cut(s) 145, 201, 628, 777, 1135, 1171
BstMAI GTCTC 7 cut(s) 102, 668, 670, 682, 703, 875, 1016
BstMBI GATC 6 cut(s) 142, 198, 625, 774, 1132, 1168
BstMWI GCNNNNNNNGC 5 cut(s) 289, 605, 649, 812, 951
BstNI CCWGG 1 cut(s) 1225
BstNSI RCATGY 4 cut(s) 539, 695, 832, 1235
BstSCI CCNGG 1 cut(s) 1223
BstSFI CTRYAG 1 cut(s) 702
BstUI CGCG 2 cut(s) 595, 597
BstV1I GCAGC 5 cut(s) 44, 124, 909, 931, 1249
BstV2I GAAGAC 1 cut(s) 1201
BstX2I RGATCY 2 cut(s) 625, 1132
BstYI RGATCY 2 cut(s) 625, 1132
Bsu36I CCTNAGG 1 cut(s) 1173
BsuI GTATCC 1 cut(s) 1008
BsuRI GGCC 2 cut(s) 567, 1040
BtgI CCRYGG 2 cut(s) 562, 669
BtsCI GGATG 7 cut(s) 91, 327, 384, 808, 814, 1104, 1171
BveI ACCTGC 1 cut(s) 473
Cac8I GCNNGC 3 cut(s) 294, 597, 1153
CfoI GCGC 4 cut(s) 37, 511, 597, 599
Cfr10I RCCGGY 1 cut(s) 37
Cfr13I GGNCC 4 cut(s) 362, 566, 719, 886
Csp6I GTAC 3 cut(s) 107, 462, 754
CviQI GTAC 3 cut(s) 107, 462, 754
DdeI CTNAG 3 cut(s) 621, 1173, 1270
DpnI GATC 6 cut(s) 144, 200, 627, 776, 1134, 1170
DpnII GATC 6 cut(s) 142, 198, 625, 774, 1132, 1168
DrdI GACNNNNNNGTC 1 cut(s) 717
DseDI GACNNNNNNGTC 1 cut(s) 717
Eam1104I CTCTTC 1 cut(s) 105
EarI CTCTTC 1 cut(s) 105
Ecl136II GAGCTC 1 cut(s) 1106
Eco130I CCWWGG 1 cut(s) 562
Eco24I GRGCYC 1 cut(s) 1108
Eco31I GGTCTC 2 cut(s) 668, 670
Eco47I GGWCC 3 cut(s) 362, 719, 886
Eco47III AGCGCT 1 cut(s) 510
Eco53kI GAGCTC 1 cut(s) 1106
Eco57I CTGAAG 1 cut(s) 591
Eco81I CCTNAGG 1 cut(s) 1173
EcoICRI GAGCTC 1 cut(s) 1106
EcoRI GAATTC 1 cut(s) 1246
EcoRII CCWGG 1 cut(s) 1223
EcoT14I CCWWGG 1 cut(s) 562
EcoT38I GRGCYC 1 cut(s) 1108
ErhI CCWWGG 1 cut(s) 562
Esp3I CGTCTC 1 cut(s) 875
FaqI GGGAC 1 cut(s) 401
FauNDI CATATG 1 cut(s) 793
FblI GTMKAC 1 cut(s) 439
Fnu4HI GCNGC 9 cut(s) 33, 138, 284, 287, 290, 385, 920, 923, 1238
FokI GGATG 7 cut(s) 98, 314, 391, 815, 821, 1111, 1178
FriOI GRGCYC 1 cut(s) 1108
Fsp4HI GCNGC 9 cut(s) 33, 138, 284, 287, 290, 385, 920, 923, 1238
FspBI CTAG 3 cut(s) 18, 78, 1220
GlaI GCGC 4 cut(s) 36, 510, 596, 598
GluI GCNGC 9 cut(s) 33, 138, 284, 287, 290, 385, 920, 923, 1238
HaeII RGCGCY 2 cut(s) 38, 512
HaeIII GGCC 2 cut(s) 567, 1040
HapII CCGG 3 cut(s) 38, 119, 1046
HhaI GCGC 4 cut(s) 37, 511, 597, 599
Hin6I GCGC 4 cut(s) 35, 509, 595, 597
HinP1I GCGC 4 cut(s) 35, 509, 595, 597
HincII GTYRAC 1 cut(s) 440
HindII GTYRAC 1 cut(s) 440
HindIII AAGCTT 1 cut(s) 1267
HinfI GANTC 7 cut(s) 91, 112, 236, 698, 934, 968, 1063
HpaII CCGG 3 cut(s) 38, 119, 1046
HphI GGTGA 2 cut(s) 245, 490
Hpy166II GTNNAC 3 cut(s) 440, 1006, 1190
Hpy188I TCNGA 8 cut(s) 258, 622, 849, 880, 967, 980, 1084, 1125
Hpy188III TCNNGA 3 cut(s) 365, 588, 731
Hpy8I GTNNAC 3 cut(s) 440, 1006, 1190
Hpy99I CGWCG 2 cut(s) 262, 786
HpyAV CCTTC 5 cut(s) 69, 151, 158, 644, 1146
HpyCH4III ACNGT 2 cut(s) 501, 1010
HpyCH4IV ACGT 2 cut(s) 756, 784
HpyCH4V TGCA 8 cut(s) 70, 309, 685, 815, 925, 1076, 1198, 1237
HpyF10VI GCNNNNNNNGC 5 cut(s) 289, 605, 649, 812, 951
HpyF3I CTNAG 3 cut(s) 621, 1173, 1270
HpySE526I ACGT 2 cut(s) 756, 784
HspAI GCGC 4 cut(s) 35, 509, 595, 597
Kzo9I GATC 6 cut(s) 142, 198, 625, 774, 1132, 1168
LmnI GCTCC 1 cut(s) 392
Lsp1109I GCAGC 5 cut(s) 44, 124, 909, 931, 1249
LweI GCATC 5 cut(s) 208, 336, 793, 802, 831
MaeI CTAG 3 cut(s) 18, 78, 1220
MaeII ACGT 2 cut(s) 756, 784
MaeIII GTNAC 1 cut(s) 173
MalI GATC 6 cut(s) 144, 200, 627, 776, 1134, 1170
MboI GATC 6 cut(s) 142, 198, 625, 774, 1132, 1168
MboII GAAGA 6 cut(s) 92, 231, 436, 584, 853, 1201
MfeI CAATTG 1 cut(s) 10
MflI RGATCY 2 cut(s) 625, 1132
MhlI GDGCHC 1 cut(s) 1108
MlyI GAGTC 1 cut(s) 1072
MmeI TCCRAC 1 cut(s) 281
MroXI GAANNNNTTC 1 cut(s) 339
MseI TTAA 6 cut(s) 488, 531, 743, 992, 1023, 1279
MslI CAYNNNNRTG 2 cut(s) 827, 833
MspA1I CMGCKG 1 cut(s) 922
MspI CCGG 3 cut(s) 38, 119, 1046
MspR9I CCNGG 1 cut(s) 1225
MunI CAATTG 1 cut(s) 10
Mva1269I GAATGC 1 cut(s) 903
MvaI CCWGG 1 cut(s) 1225
MvnI CGCG 2 cut(s) 595, 597
MwoI GCNNNNNNNGC 5 cut(s) 289, 605, 649, 812, 951
NcoI CCATGG 1 cut(s) 562
NdeI CATATG 1 cut(s) 793
NdeII GATC 6 cut(s) 142, 198, 625, 774, 1132, 1168
NlaIV GGNNCC 2 cut(s) 388, 888
NmuCI GTSAC 1 cut(s) 173
NspI RCATGY 4 cut(s) 539, 695, 832, 1235
NspV TTCGAA 1 cut(s) 242
PauI GCGCGC 1 cut(s) 595
PciI ACATGT 1 cut(s) 1231
PctI GAATGC 1 cut(s) 903
PdmI GAANNNNTTC 1 cut(s) 339
PfeI GAWTC 6 cut(s) 91, 112, 236, 698, 934, 968
PflMI CCANNNNNTGG 3 cut(s) 379, 475, 563
PkrI GCNGC 9 cut(s) 34, 139, 285, 288, 291, 386, 921, 924, 1239
PleI GAGTC 1 cut(s) 1071
PpsI GAGTC 1 cut(s) 1071
PscI ACATGT 1 cut(s) 1231
Psp124BI GAGCTC 1 cut(s) 1108
Psp6I CCWGG 1 cut(s) 1223
PspGI CCWGG 1 cut(s) 1223
PspN4I GGNNCC 2 cut(s) 388, 888
PspPI GGNCC 4 cut(s) 362, 566, 719, 886
PsuI RGATCY 2 cut(s) 625, 1132
PteI GCGCGC 1 cut(s) 595
PvuII CAGCTG 1 cut(s) 922
RsaI GTAC 3 cut(s) 108, 463, 755
RsaNI GTAC 3 cut(s) 107, 462, 754
RseI CAYNNNNRTG 2 cut(s) 827, 833
SacI GAGCTC 1 cut(s) 1108
SalI GTCGAC 1 cut(s) 438
SaqAI TTAA 6 cut(s) 488, 531, 743, 992, 1023, 1279
SatI GCNGC 9 cut(s) 33, 138, 284, 287, 290, 385, 920, 923, 1238
Sau3AI GATC 6 cut(s) 142, 198, 625, 774, 1132, 1168
Sau96I GGNCC 4 cut(s) 362, 566, 719, 886
SchI GAGTC 1 cut(s) 1072
ScrFI CCNGG 1 cut(s) 1225
SduI GDGCHC 1 cut(s) 1108
SfaNI GCATC 5 cut(s) 208, 336, 793, 802, 831
SfcI CTRYAG 1 cut(s) 702
SfuI TTCGAA 1 cut(s) 242
SinI GGWCC 3 cut(s) 362, 719, 886
SmiMI CAYNNNNRTG 2 cut(s) 827, 833
SmlI CTYRAG 1 cut(s) 1264
SmoI CTYRAG 1 cut(s) 1264
SsiI CCGC 5 cut(s) 283, 286, 289, 384, 806
SspMI CTAG 3 cut(s) 18, 78, 1220
SstI GAGCTC 1 cut(s) 1108
StyD4I CCNGG 1 cut(s) 1223
StyI CCWWGG 1 cut(s) 562
TaaI ACNGT 2 cut(s) 501, 1010
TaiI ACGT 2 cut(s) 759, 787
TaqI TCGA 4 cut(s) 155, 242, 439, 781
TatI WGTACW 1 cut(s) 106
TauI GCSGC 4 cut(s) 286, 289, 292, 387
TfiI GAWTC 6 cut(s) 91, 112, 236, 698, 934, 968
Tru1I TTAA 6 cut(s) 488, 531, 743, 992, 1023, 1279
Tru9I TTAA 6 cut(s) 488, 531, 743, 992, 1023, 1279
TseFI GTSAC 1 cut(s) 173
TseI GCWGC 5 cut(s) 32, 137, 919, 922, 1237
Tsp45I GTSAC 1 cut(s) 173
TspDTI ATGAA 4 cut(s) 257, 810, 1128, 1147
TspGWI ACGGA 2 cut(s) 658, 899
Van91I CCANNNNNTGG 3 cut(s) 379, 475, 563
VpaK11BI GGWCC 3 cut(s) 362, 719, 886
XapI RAATTY 3 cut(s) 430, 1070, 1246
XceI RCATGY 4 cut(s) 539, 695, 832, 1235
XmiI GTMKAC 1 cut(s) 439
XmnI GAANNNNTTC 1 cut(s) 339
XspI CTAG 3 cut(s) 18, 78, 1220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.