Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0001070

Physical Location & Seq

Genomic Coordinates Reverse (-)

12678634 .. 12683490

Gene Structure 4857 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2520 bp

ATGCTTGCATCTGGAGGAAAAGATAATGACATTATATTATGGGATGTGGTTGGTGAGACCGGCCTATATCGCCTCCGTGGCCATCGAGATCAGGTGACTGACCTCCTTTTCCTGGATTCTGGTAAAAAACTCGTCAGTTCCTCAAAAGATAAATTTTTGAGGGTATGGGATCTTGATACCCAGCACTGCATGCAGACAATTAGTGGCCACCATAGCGAAATTTGGTCCATAGACAGTGATGCAGAAGAAAGATATTTGGTCACTGGGTCTTCGGATCCAGAACTTAGATTTTACACAATTAAGCATGAAGTGATACATGAGGAATCCATTTCTAATATAGGCGAAAATAAAATTGTGAATGATGGAGAGTCATTGACCAAAAATAAATGGGAAGTTTTGAAACTGTTTGGTGAAGTTCAGCGACAAAGCAAGGATAGAGTTGCAACTGTGAGATTCAACAAGTCTGGTAATTTGTTGGCTTGTCAAGTGGCAGGTAAGACAGTAGATGTATTCCAGGTACTAGATGAGGCTGAATCAAAGCGTAAGGCAAAACGTAGGCTTCACCGGAAGAAAGAAAAAAAATCTGCAAAAGGGGCAACTGAGGTGATGGAAAATGGCGACACAAATCACGGAGCTGGAGAAGATGGAAATAGTCCTGTGGTTACGGTTCCTGATGTCTTCAAGCTTCTTCAAACTATTCGAGCTAGCAAAAAGATATGCTCCATCTCTTTCTGTCCTAACACTCCAAAGAGTTTGCTAGCTACAATAGCTTTGTCTTTGAACAATAATTCATTGGAATTTTATTCGATTGAAAGCAAAGCAACAACAAAAACACATGCTATAGAGCTCCAAGGACACCGGTCTGATGTCAGAAGTGTTACACTTAGCTCAGACAACACGCTCTTGATGTCAACTAGTCACAATGCGGTAAAGATTTGGAATCCAAGTACTGGTTCCTGCCTTCGAACTATTGATTCTGGATATGGCATGTGTGGTTTTATCGTTCCTAATAGCAAGTATGCACTTGTCGGAACTAAAGGCGGAACAGTGGAAATTATTGACATTGGAAGTGGCACTTGCATTGAAGTAGTGGAAGCTCATGGTGGCACTGTTGGCTCCATTGCAGCCATTCCTAATGAAAATGGTTTTGTCACAGGAAGTGCAGATCACGATGTGAAGTTTTGGGAATACCAAGTTAAACAAAAGTCTCCCCAAGACTCTAAGCACCTAACGGTGTCAAATGTAAGGACTATGAAGATGAATGATGATGTCCTCGTGGTTGCTGTCAGCCCTGATGCAAAATATGTTTTGGTTGCTCTGTTGGACTGCACAGTGAAGGTTTACTATATGGATACATTGAAACCTTTCCTTTCCTTATATGGTCACAAGCTGCCTGTTCTGTGTATGGATTTATCATCTGATGGCGATCTATTAGTGACTGGATCTGCTGACAAAAATTTGAAGATTTGGGGTTTAGATTTCGGTGATTGTCATAAATCCATATTTGCTCACGCTGACAGTGTCATGGCGGTGCAATTTGTACGAAATACACATTACATGTTTAGTGTCGGGAAAGATCGCCTAGTCAAATACTGGGATGCTGATAAATTTGAGTTGCTTTTAACTCTTGAGGGACACCATGCTGATGTTTGGTGCCTTGGAATTAGTAACCGTGGTGATTTCATTGTTACGGGATCTCATGACCGATCAATACGTCGTTGGGATCGTACTGAAGAGCCCTTTTTTATCGAGGAAGAGAAAGAGAAAAGGTTGGAAGAGATGTTCGAGTCTGACCTCGATAATGCATTTGAGAACAAGTATGCACCCACGGAAGAAATTCCTGAAGAGGGAGCTGTGGCCTTAGCAGGGAAGAAATCAAAAGAAACAATTACAGCTACTGATTCAATTATTGACGCACTAGATATAGCAGAGGTAGAATTGAAGCGAATCGCCGAACATGAGGAGGAGAAAAGCAAGGGGAAAGTTGCTGAGTTACAACCAAATGTTGTTTTGCTTGGGCTTTCTCCATCTGATTATGTTCTTCGTGCCCTTTCAAATGTTCAGACAAATGATCTGGAACAGACGCTACTGGCTCTATCATTCTCAGACGCTTTGAAGCTCTTGTCTTTCTTGAAGGACTGGACTTCAAATCCTGATAAGATTGAACTCGTTTGCAGGGTTTGTACATTCCTGGTGCAGATACATTATAATCAGTTACTTAGTACGCTGGCGGCCAGACTTGTGTTGTCAGAGTTGGAAGAGATTTATCCAAGAGTTAAGGAATGCAAAGATACTATTGGTTACAACATTGCAGCGATGGAGCATATTAAGCAACTGAGGGCTTTGAAATCAGGTGGATTGTTTCAAGATGCAAAATCAAAGTTACAGGAAATTCGTGCGCAGCATTCTAAGCGTCTGGAAGCAAGGACAGATACGAGAGAAGAAAATGGAAGAAAAAGGAGGAAAAAGAGCAAGAGCAAGAATCCAAGTGACGTACATGTTAGATTGTTCGACAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005654 GO:0005730 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0012505 GO:0016020 GO:0016070 GO:0016072 GO:0022613 GO:0030490 GO:0030515 GO:0030684 GO:0030686 GO:0031090 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032040 GO:0032991 GO:0034388 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042274 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

Utp12 WD40

Protein Analysis

840

Amino Acids

93.82

Weight (kDa)

6.26

Isoelectric Point (pI)

40.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WDR55	PF24796	1 - 106	2.2e-08	WDR55
WD40_WDHD1_1st	PF24817	1 - 68	3.3e-07	WDHD1 first WD40 domain
Beta-prop_SCAP	PF24017	2 - 61	4.6e-06	SCAP Beta-propeller
Beta-prop_WDR3_2nd	PF25172	2 - 103	1.1e-13	WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	2 - 98	2.5e-06	WDR36/Utp21 first beta-propeller
Beta-prop_TEP1_2nd	PF25047	2 - 98	7.6e-09	TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	2 - 63	2.6e-06	WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2	PF23414	2 - 102	6.4e-07	Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz	PF24807	2 - 100	1.2e-09	CDC20/Fizzy WD40 domain
WD40_Prp19	PF24814	2 - 104	7.8e-18	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	2 - 109	7e-15	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	2 - 100	2.6e-43	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	2 - 102	8.8e-18	WDR5 beta-propeller domain
WD40_Gbeta	PF25391	7 - 102	2.8e-07	G protein beta WD-40 repeat protein
WD40	PF00400	22 - 57	1.8e-06	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	24 - 138	5.1e-09	CAF1B/HIR1 beta-propeller domain
EIF3I	PF24805	25 - 102	1.4e-09	EIF3I
Beta-prop_WDR3_2nd	PF25172	57 - 171	1.5e-06	WDR3 second beta-propeller domain
WD40_CDC20-Fz	PF24807	142 - 333	1.3e-06	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	143 - 401	5.2e-17	WDR5 beta-propeller domain
WD40_Prp19	PF24814	231 - 395	2.2e-10	Prp19 WD40 domain
WDR55	PF24796	232 - 404	3.9e-08	WDR55
Beta-prop_WDR3_1st	PF25173	237 - 396	1.1e-14	WDR3 first beta-propeller domain
Beta-prop_THOC3	PF25174	261 - 500	5.1e-26	THOC3 beta-propeller domain
WD40_Gbeta	PF25391	262 - 490	6.9e-13	G protein beta WD-40 repeat protein
Beta-prop_WDR3_2nd	PF25172	289 - 580	1.6e-135	WDR3 second beta-propeller domain
WD40_CDC20-Fz	PF24807	308 - 574	1.5e-25	CDC20/Fizzy WD40 domain
WD40_Prp19	PF24814	338 - 534	1e-23	Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	339 - 527	1.1e-12	WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2	PF23414	374 - 538	1.8e-14	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd	PF25047	380 - 577	1.7e-15	TEP-1 second beta-propeller
WD40_MABP1-WDR62_2nd	PF24782	383 - 532	1e-10	MABP1/WDR62 second WD40 domain
WDR55	PF24796	417 - 533	1.8e-07	WDR55
WD40_WDHD1_1st	PF24817	418 - 582	2.7e-16	WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_1st	PF25171	419 - 533	2.5e-07	WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st	PF25173	419 - 575	3e-30	WDR3 first beta-propeller domain
WD40_Gbeta	PF25391	421 - 574	4.6e-10	G protein beta WD-40 repeat protein
Beta-prop_WDR5	PF25175	422 - 577	2.3e-28	WDR5 beta-propeller domain
WD40	PF00400	455 - 490	2.5e-08	WD domain, G-beta repeat
Beta-prop_THOC3	PF25174	498 - 575	4.2e-12	THOC3 beta-propeller domain
EIF3I	PF24805	503 - 576	7.2e-09	EIF3I
WD40_Prp19	PF24814	524 - 590	7.7e-06	Prp19 WD40 domain
WD40	PF00400	538 - 575	1.6e-08	WD domain, G-beta repeat
Utp12	PF04003	677 - 778	5e-20	Dip2/Utp12 Family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001070)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G21540
fragaria_vesca	FvH4_6g16900 FvH4_6g16900 FvH4_6g16900 FvH4_6g16900 FvH4_6g16900
malus_domestica	MD12G1106200.v1.1
prunus_persica	Prupe.7G125100_v2.0.a1
pyrus_communis	pycom12g10640
rosa_chinensis	RchiOBHm_Chr3g0470921 RchiOBHm_Chr7g0191891 RchiOBHm_Chr7g0191911 RchiOBHm_Chr7g0224541
rosa_laevigata	RLG00000001887 RLG00000004419 RLG00000004420 RLG00000017852 RLG00000024233
rosa_multiflora	Rmu_co8045794.1_g000001 Rmu_co8107512.1_g000001 Rmu_co8259631.1_g000001 Rmu_co8279299.1_g000001 Rmu_co8395733.1_g000001 Rmu_sc0003361.1_g000007 Rmu_sc0003527.1_g000004 Rmu_sc0005264.1_g000005 Rmu_sc0023809.1_g000001 Rmu_sc0037201.1_g000001 Rmu_sc0037202.1_g000001 Rmu_sc0038402.1_g000001 Rmu_sc0038402.1_g000003 Rmu_sc0039860.1_g000001 Rmu_ssc0000421.1_g000010
rosa_roxburghii	Rroxscaffold_3G00234650 Rroxscaffold_3G00263480 Rroxscaffold_3G00263490 Rroxscaffold_6G00410410
rosa_rugosa	Rorug03G0113900 Rorug06G0511700 Rorug06G0511700 Rorug07G0172700 Rorug07G0172800 Rorug07G0182600
rosa_samantha	Rh2CG630200 Rh2DG680600 Rh3AG163600 Rh3BG189100 Rh3CG179100 Rh3DG187100 Rh5AG328900 Rh7AG121800 Rh7AG378900 Rh7BG123300 Rh7BG123400 Rh7CG398600 Rh7DG124900 Rh7DG369600
rosa_wichuraiana	Rw3G015400 Rw7G010370 Rw7G031220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2208
Acc16I	TGCGCA	1 cut(s)	2400
Acc36I	ACCTGC	1 cut(s)	482
AccB1I	GGYRCC	1 cut(s)	1653
AccB7I	CCANNNNNTGG	1 cut(s)	950
AciI	CCGC	4 cut(s)	926, 1041, 1529, 2231
AclWI	GGATC	6 cut(s)	177, 269, 282, 1450, 1702, 1731
AcoI	YGGCCR	3 cut(s)	79, 205, 2232
AcsI	RAATTY	7 cut(s)	152, 219, 797, 1456, 1607, 1836, 2391
AcuI	CTGAAG	2 cut(s)	1752, 1863
AdeI	CACNNNGTG	1 cut(s)	1174
AfaI	GTAC	7 cut(s)	519, 949, 1542, 1729, 2185, 2224, 2496
AfiI	CCNNNNNNNGG	4 cut(s)	112, 950, 1847, 1866
AflIII	ACRYGT	2 cut(s)	1557, 2497
AgeI	ACCGGT	1 cut(s)	858
AhlI	ACTAGT	1 cut(s)	914
AjnI	CCWGG	3 cut(s)	111, 513, 2190
AjuI	GAANNNNNNNTTGG	2 cut(s)	937, 969
AloI	GAACNNNNNNTCC	2 cut(s)	1766, 1798
Alw21I	GWGCWC	1 cut(s)	849
Alw26I	GTCTC	2 cut(s)	50, 1212
AlwI	GGATC	6 cut(s)	177, 269, 282, 1450, 1702, 1731
AlwNI	CAGNNNCTG	1 cut(s)	1898
AoxI	GGCC	5 cut(s)	61, 79, 205, 1857, 2232
ApeKI	GCWGC	4 cut(s)	1124, 1390, 2312, 2401
ApoI	RAATTY	7 cut(s)	152, 219, 797, 1456, 1607, 1836, 2391
AsiGI	ACCGGT	1 cut(s)	858
Asp700I	GAANNNNTTC	2 cut(s)	1364, 1836
AspLEI	GCGC	1 cut(s)	2401
AspS9I	GGNCC	1 cut(s)	225
AsuHPI	GGTGA	7 cut(s)	65, 106, 422, 554, 616, 1496, 1688
AsuII	TTCGAA	1 cut(s)	964
AsuNHI	GCTAGC	2 cut(s)	704, 757
AvaII	GGWCC	1 cut(s)	225
BaeGI	GKGCMC	1 cut(s)	2050
BalI	TGGCCA	2 cut(s)	81, 207
BamHI	GGATCC	1 cut(s)	274
BanI	GGYRCC	1 cut(s)	1653
BanII	GRGCYC	2 cut(s)	849, 1740
BauI	CACGAG	1 cut(s)	1274
BbsI	GAAGAC	2 cut(s)	261, 670
Bbv12I	GWGCWC	1 cut(s)	849
BbvI	GCAGC	4 cut(s)	1136, 1377, 2324, 2413
BccI	CCATC	8 cut(s)	90, 356, 601, 638, 731, 1415, 2035, 2311
BciT130I	CCWGG	3 cut(s)	113, 515, 2192
BciVI	GTATCC	1 cut(s)	1345
BcoDI	GTCTC	2 cut(s)	50, 1212
BcuI	ACTAGT	1 cut(s)	914
BfaI	CTAG	6 cut(s)	521, 705, 758, 915, 1583, 1919
BfmI	CTRYAG	1 cut(s)	840
BfuAI	ACCTGC	1 cut(s)	482
BfuI	GTATCC	1 cut(s)	1345
BglI	GCCNNNNNGGC	1 cut(s)	78
BisI	GCNGC	5 cut(s)	1125, 1391, 2232, 2313, 2402
BlsI	GCNGC	5 cut(s)	1126, 1392, 2233, 2314, 2403
BmcAI	AGTACT	1 cut(s)	949
Bme1390I	CCNGG	3 cut(s)	113, 515, 2192
Bme18I	GGWCC	1 cut(s)	225
BmgT120I	GGNCC	1 cut(s)	225
BmiI	GGNNCC	5 cut(s)	276, 669, 955, 1117, 1655
BmrFI	CCNGG	3 cut(s)	113, 515, 2192
BmrI	ACTGGG	2 cut(s)	273, 1603
BmsI	GCATC	5 cut(s)	17, 229, 1285, 1588, 2359
BmtI	GCTAGC	2 cut(s)	708, 761
BmuI	ACTGGG	2 cut(s)	273, 1603
BoxI	GACNNNNGTC	1 cut(s)	859
BpiI	GAAGAC	2 cut(s)	261, 670
BpmI	CTGGAG	2 cut(s)	33, 657
Bpu10I	CCTNAGC	1 cut(s)	1861
Bpu14I	TTCGAA	1 cut(s)	964
BpuEI	CTTGAG	1 cut(s)	1649
BsaBI	GATNNNNATC	1 cut(s)	1425
BsaI	GGTCTC	1 cut(s)	50
BsaJI	CCNNGG	5 cut(s)	76, 850, 1657, 1672, 1827
BsaWI	WCCGGW	2 cut(s)	564, 858
Bsc4I	CCNNNNNNNGG	4 cut(s)	112, 950, 1847, 1866
Bse118I	RCCGGY	2 cut(s)	59, 858
Bse1I	ACTGG	6 cut(s)	268, 955, 1444, 1598, 2094, 2144
Bse3DI	GCAATG	2 cut(s)	1119, 2307
Bse8I	GATNNNNATC	1 cut(s)	1425
BseBI	CCWGG	3 cut(s)	113, 515, 2192
BseDI	CCNNGG	5 cut(s)	76, 850, 1657, 1672, 1827
BseGI	GGATG	2 cut(s)	49, 1603
BseJI	GATNNNNATC	1 cut(s)	1425
BseLI	CCNNNNNNNGG	4 cut(s)	112, 950, 1847, 1866
BseMI	GCAATG	2 cut(s)	1119, 2307
BseMII	CTCAG	5 cut(s)	591, 903, 1980, 2118, 2327
BseNI	ACTGG	6 cut(s)	268, 955, 1444, 1598, 2094, 2144
BseRI	GAGGAG	2 cut(s)	1976, 1979
BseSI	GKGCMC	1 cut(s)	2050
BseXI	GCAGC	4 cut(s)	1136, 1377, 2324, 2413
BseYI	CCCAGC	1 cut(s)	180
BsgI	GTGCAG	3 cut(s)	1182, 1312, 2216
BshFI	GGCC	5 cut(s)	63, 81, 207, 1859, 2234
BshNI	GGYRCC	1 cut(s)	1653
BshTI	ACCGGT	1 cut(s)	858
BsiHKAI	GWGCWC	1 cut(s)	849
BsiSI	CCGG	3 cut(s)	60, 565, 859
BslFI	GGGAC	1 cut(s)	1647
BslI	CCNNNNNNNGG	4 cut(s)	112, 950, 1847, 1866
BsmAI	GTCTC	2 cut(s)	50, 1212
BsmFI	GGGAC	1 cut(s)	1647
BsmI	GAATGC	2 cut(s)	2288, 2404
BsnI	GGCC	5 cut(s)	63, 81, 207, 1859, 2234
Bso31I	GGTCTC	1 cut(s)	50
Bsp119I	TTCGAA	1 cut(s)	964
Bsp1286I	GDGCHC	3 cut(s)	849, 1740, 2050
Bsp1407I	TGTACA	1 cut(s)	2183
BspACI	CCGC	4 cut(s)	926, 1041, 1529, 2231
BspANI	GGCC	5 cut(s)	63, 81, 207, 1859, 2234
BspCNI	CTCAG	5 cut(s)	592, 902, 1981, 2117, 2328
BspHI	TCATGA	1 cut(s)	1699
BspLI	GGNNCC	5 cut(s)	276, 669, 955, 1117, 1655
BspMI	ACCTGC	1 cut(s)	482
BspOI	GCTAGC	2 cut(s)	708, 761
BspPI	GGATC	6 cut(s)	177, 269, 282, 1450, 1702, 1731
BspQI	GCTCTTC	1 cut(s)	1728
BspT104I	TTCGAA	1 cut(s)	964
BspT107I	GGYRCC	1 cut(s)	1653
BspTNI	GGTCTC	1 cut(s)	50
BsrDI	GCAATG	2 cut(s)	1119, 2307
BsrFI	RCCGGY	2 cut(s)	59, 858
BsrGI	TGTACA	1 cut(s)	2183
BsrI	ACTGG	6 cut(s)	268, 955, 1444, 1598, 2094, 2144
BssAI	RCCGGY	2 cut(s)	59, 858
BssECI	CCNNGG	5 cut(s)	76, 850, 1657, 1672, 1827
BssSI	CACGAG	1 cut(s)	1274
BssT1I	CCWWGG	2 cut(s)	850, 1657
Bst2BI	CACGAG	1 cut(s)	1274
Bst2UI	CCWGG	3 cut(s)	113, 515, 2192
Bst6I	CTCTTC	5 cut(s)	1728, 1749, 1770, 1839, 2253
BstAPI	GCANNNNNTGC	1 cut(s)	190
BstAUI	TGTACA	1 cut(s)	2183
BstBI	TTCGAA	1 cut(s)	964
BstC8I	GCNNGC	5 cut(s)	6, 191, 706, 759, 2229
BstDSI	CCRYGG	3 cut(s)	76, 1672, 1827
BstF5I	GGATG	2 cut(s)	49, 1603
BstHHI	GCGC	1 cut(s)	2401
BstMAI	GTCTC	2 cut(s)	50, 1212
BstMWI	GCNNNNNNNGC	9 cut(s)	69, 78, 190, 213, 593, 767, 1113, 2329, 2410
BstNI	CCWGG	3 cut(s)	113, 515, 2192
BstNSI	RCATGY	5 cut(s)	193, 839, 991, 1561, 2501
BstPAI	GACNNNNGTC	1 cut(s)	859
BstSCI	CCNGG	3 cut(s)	111, 513, 2190
BstSFI	CTRYAG	1 cut(s)	840
BstSLI	GKGCMC	1 cut(s)	2050
BstV1I	GCAGC	4 cut(s)	1136, 1377, 2324, 2413
BstV2I	GAAGAC	2 cut(s)	261, 670
BstX2I	RGATCY	4 cut(s)	169, 274, 1442, 1694
BstYI	RGATCY	4 cut(s)	169, 274, 1442, 1694
BsuI	GTATCC	1 cut(s)	1345
BsuRI	GGCC	5 cut(s)	63, 81, 207, 1859, 2234
BtgI	CCRYGG	3 cut(s)	76, 1672, 1827
BtgZI	GCGATG	1 cut(s)	2330
BtsCI	GGATG	2 cut(s)	49, 1603
BtsI	GCAGTG	1 cut(s)	184
BtsIMutI	CAGTG	7 cut(s)	184, 241, 261, 1053, 1107, 1338, 1525
BveI	ACCTGC	1 cut(s)	482
Cac8I	GCNNGC	5 cut(s)	6, 191, 706, 759, 2229
CaiI	CAGNNNCTG	1 cut(s)	1898
CciI	TCATGA	1 cut(s)	1699
CfoI	GCGC	1 cut(s)	2401
Cfr10I	RCCGGY	2 cut(s)	59, 858
Cfr13I	GGNCC	1 cut(s)	225
CseI	GACGC	4 cut(s)	1922, 2092, 2117, 2402
Csp6I	GTAC	7 cut(s)	518, 948, 1541, 1728, 2184, 2223, 2495
CspAI	ACCGGT	1 cut(s)	858
CviQI	GTAC	7 cut(s)	518, 948, 1541, 1728, 2184, 2223, 2495
DraIII	CACNNNGTG	1 cut(s)	1174
EaeI	YGGCCR	3 cut(s)	79, 205, 2232
Eam1104I	CTCTTC	5 cut(s)	1728, 1749, 1770, 1839, 2253
EarI	CTCTTC	5 cut(s)	1728, 1749, 1770, 1839, 2253
EciI	GGCGGA	1 cut(s)	1056
Ecl136II	GAGCTC	1 cut(s)	847
Eco130I	CCWWGG	2 cut(s)	850, 1657
Eco24I	GRGCYC	2 cut(s)	849, 1740
Eco31I	GGTCTC	1 cut(s)	50
Eco47I	GGWCC	1 cut(s)	225
Eco53kI	GAGCTC	1 cut(s)	847
Eco57I	CTGAAG	2 cut(s)	1752, 1863
EcoICRI	GAGCTC	1 cut(s)	847
EcoRII	CCWGG	3 cut(s)	111, 513, 2190
EcoT14I	CCWWGG	2 cut(s)	850, 1657
EcoT22I	ATGCAT	1 cut(s)	1807
EcoT38I	GRGCYC	2 cut(s)	849, 1740
ErhI	CCWWGG	2 cut(s)	850, 1657
FalI	AAGNNNNNCTT	2 cut(s)	1060, 1092
FaqI	GGGAC	1 cut(s)	1647
Fnu4HI	GCNGC	5 cut(s)	1125, 1391, 2232, 2313, 2402
FokI	GGATG	2 cut(s)	56, 1610
FriOI	GRGCYC	2 cut(s)	849, 1740
Fsp4HI	GCNGC	5 cut(s)	1125, 1391, 2232, 2313, 2402
FspBI	CTAG	6 cut(s)	521, 705, 758, 915, 1583, 1919
FspI	TGCGCA	1 cut(s)	2400
GlaI	GCGC	1 cut(s)	2400
GluI	GCNGC	5 cut(s)	1125, 1391, 2232, 2313, 2402
GsaI	CCCAGC	1 cut(s)	184
GsuI	CTGGAG	2 cut(s)	33, 657
HaeIII	GGCC	5 cut(s)	63, 81, 207, 1859, 2234
HapII	CCGG	3 cut(s)	60, 565, 859
HgaI	GACGC	4 cut(s)	1922, 2092, 2117, 2402
HhaI	GCGC	1 cut(s)	2401
Hin6I	GCGC	1 cut(s)	2399
HinP1I	GCGC	1 cut(s)	2399
HincII	GTYRAC	1 cut(s)	912
HindII	GTYRAC	1 cut(s)	912
HindIII	AAGCTT	1 cut(s)	683
HpaII	CCGG	3 cut(s)	60, 565, 859
HphI	GGTGA	7 cut(s)	65, 106, 422, 554, 616, 1496, 1688
Hpy166II	GTNNAC	2 cut(s)	912, 1342
Hpy8I	GTNNAC	2 cut(s)	912, 1342
Hpy99I	CGWCG	1 cut(s)	1719
HpyAV	CCTTC	3 cut(s)	971, 1330, 2128
HpyCH4IV	ACGT	3 cut(s)	553, 1714, 2493
HpyF10VI	GCNNNNNNNGC	9 cut(s)	69, 78, 190, 213, 593, 767, 1113, 2329, 2410
HpySE526I	ACGT	3 cut(s)	553, 1714, 2493
HspAI	GCGC	1 cut(s)	2399
LguI	GCTCTTC	1 cut(s)	1728
LmnI	GCTCC	6 cut(s)	632, 725, 852, 1121, 1850, 2320
Lsp1109I	GCAGC	4 cut(s)	1136, 1377, 2324, 2413
LweI	GCATC	5 cut(s)	17, 229, 1285, 1588, 2359
MaeI	CTAG	6 cut(s)	521, 705, 758, 915, 1583, 1919
MaeII	ACGT	3 cut(s)	553, 1714, 2493
MflI	RGATCY	4 cut(s)	169, 274, 1442, 1694
MhlI	GDGCHC	3 cut(s)	849, 1740, 2050
MlsI	TGGCCA	2 cut(s)	81, 207
MluNI	TGGCCA	2 cut(s)	81, 207
MlyI	GAGTC	3 cut(s)	377, 1211, 1796
MmeI	TCCRAC	4 cut(s)	1009, 1302, 1752, 2235
Mox20I	TGGCCA	2 cut(s)	81, 207
Mph1103I	ATGCAT	1 cut(s)	1807
MroXI	GAANNNNTTC	2 cut(s)	1364, 1836
MscI	TGGCCA	2 cut(s)	81, 207
MseI	TTAA	5 cut(s)	300, 1197, 1622, 2277, 2328
MslI	CAYNNNNRTG	2 cut(s)	1529, 1644
Msp20I	TGGCCA	2 cut(s)	81, 207
MspI	CCGG	3 cut(s)	60, 565, 859
MspR9I	CCNGG	3 cut(s)	113, 515, 2192
Mva1269I	GAATGC	2 cut(s)	2288, 2404
MvaI	CCWGG	3 cut(s)	113, 515, 2192
MwoI	GCNNNNNNNGC	9 cut(s)	69, 78, 190, 213, 593, 767, 1113, 2329, 2410
NheI	GCTAGC	2 cut(s)	704, 757
NlaIV	GGNNCC	5 cut(s)	276, 669, 955, 1117, 1655
NmuCI	GTSAC	7 cut(s)	94, 259, 917, 1150, 1382, 1435, 2489
NsbI	TGCGCA	1 cut(s)	2400
NsiI	ATGCAT	1 cut(s)	1807
NspI	RCATGY	5 cut(s)	193, 839, 991, 1561, 2501
NspV	TTCGAA	1 cut(s)	964
PaeI	GCATGC	1 cut(s)	193
PagI	TCATGA	1 cut(s)	1699
PciI	ACATGT	2 cut(s)	1557, 2497
PciSI	GCTCTTC	1 cut(s)	1728
PcsI	WCGNNNNNNNCGW	1 cut(s)	1723
PctI	GAATGC	2 cut(s)	2288, 2404
PdmI	GAANNNNTTC	2 cut(s)	1364, 1836
PfeI	GAWTC	9 cut(s)	116, 323, 453, 533, 940, 974, 1901, 1947, 2482
PflFI	GACNNNGTC	1 cut(s)	1520
PflMI	CCANNNNNTGG	1 cut(s)	950
PfoI	TCCNGGA	1 cut(s)	111
PinAI	ACCGGT	1 cut(s)	858
PkrI	GCNGC	5 cut(s)	1126, 1392, 2233, 2314, 2403
PleI	GAGTC	3 cut(s)	376, 1211, 1795
PpsI	GAGTC	3 cut(s)	376, 1211, 1795
PscI	ACATGT	2 cut(s)	1557, 2497
PshAI	GACNNNNGTC	1 cut(s)	859
PsiI	TTATAA	1 cut(s)	2208
Psp124BI	GAGCTC	1 cut(s)	849
Psp6I	CCWGG	3 cut(s)	111, 513, 2190
PspFI	CCCAGC	1 cut(s)	180
PspGI	CCWGG	3 cut(s)	111, 513, 2190
PspN4I	GGNNCC	5 cut(s)	276, 669, 955, 1117, 1655
PspPI	GGNCC	1 cut(s)	225
PsrI	GAACNNNNNNTAC	2 cut(s)	2070, 2102
PstNI	CAGNNNCTG	1 cut(s)	1898
PsuI	RGATCY	4 cut(s)	169, 274, 1442, 1694
PsyI	GACNNNGTC	1 cut(s)	1520
RsaI	GTAC	7 cut(s)	519, 949, 1542, 1729, 2185, 2224, 2496
RsaNI	GTAC	7 cut(s)	518, 948, 1541, 1728, 2184, 2223, 2495
RseI	CAYNNNNRTG	2 cut(s)	1529, 1644
SacI	GAGCTC	1 cut(s)	849
SapI	GCTCTTC	1 cut(s)	1728
SaqAI	TTAA	5 cut(s)	300, 1197, 1622, 2277, 2328
SatI	GCNGC	5 cut(s)	1125, 1391, 2232, 2313, 2402
Sau96I	GGNCC	1 cut(s)	225
ScaI	AGTACT	1 cut(s)	949
SchI	GAGTC	3 cut(s)	377, 1211, 1796
ScrFI	CCNGG	3 cut(s)	113, 515, 2192
SduI	GDGCHC	3 cut(s)	849, 1740, 2050
SfaNI	GCATC	5 cut(s)	17, 229, 1285, 1588, 2359
SfcI	CTRYAG	1 cut(s)	840
SfuI	TTCGAA	1 cut(s)	964
SinI	GGWCC	1 cut(s)	225
SmiMI	CAYNNNNRTG	2 cut(s)	1529, 1644
SmlI	CTYRAG	1 cut(s)	1628
SmoI	CTYRAG	1 cut(s)	1628
SpeI	ACTAGT	1 cut(s)	914
SphI	GCATGC	1 cut(s)	193
SsiI	CCGC	4 cut(s)	926, 1041, 1529, 2231
SspMI	CTAG	6 cut(s)	521, 705, 758, 915, 1583, 1919
SstI	GAGCTC	1 cut(s)	849
StyD4I	CCNGG	3 cut(s)	111, 513, 2190
StyI	CCWWGG	2 cut(s)	850, 1657
TaiI	ACGT	3 cut(s)	556, 1717, 2496
TaqI	TCGA	8 cut(s)	85, 700, 806, 964, 1749, 1785, 1797, 2511
TaqII	GACCGA	1 cut(s)	1719
TatI	WGTACW	2 cut(s)	947, 2183
TauI	GCSGC	1 cut(s)	2234
TfiI	GAWTC	9 cut(s)	116, 323, 453, 533, 940, 974, 1901, 1947, 2482
Tru1I	TTAA	5 cut(s)	300, 1197, 1622, 2277, 2328
Tru9I	TTAA	5 cut(s)	300, 1197, 1622, 2277, 2328
TscAI	CASTG	7 cut(s)	191, 241, 268, 1053, 1114, 1338, 1525
TseFI	GTSAC	7 cut(s)	94, 259, 917, 1150, 1382, 1435, 2489
TseI	GCWGC	4 cut(s)	1124, 1390, 2312, 2401
Tsp45I	GTSAC	7 cut(s)	94, 259, 917, 1150, 1382, 1435, 2489
TspDTI	ATGAA	6 cut(s)	321, 780, 1152, 1268, 1274, 1672
TspGWI	ACGGA	3 cut(s)	65, 645, 1844
TspRI	CASTG	7 cut(s)	191, 241, 268, 1053, 1114, 1338, 1525
Tth111I	GACNNNGTC	1 cut(s)	1520
Van91I	CCANNNNNTGG	1 cut(s)	950
VpaK11BI	GGWCC	1 cut(s)	225
XapI	RAATTY	7 cut(s)	152, 219, 797, 1456, 1607, 1836, 2391
XceI	RCATGY	5 cut(s)	193, 839, 991, 1561, 2501
XmnI	GAANNNNTTC	2 cut(s)	1364, 1836
XspI	CTAG	6 cut(s)	521, 705, 758, 915, 1583, 1919
ZrmI	AGTACT	1 cut(s)	949
Zsp2I	ATGCAT	1 cut(s)	1807

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.