Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr3

Orthogroup ID

OG0001070

Physical Location & Seq

Genomic Coordinates Forward (+)

15869285 .. 15876568

Gene Structure 7284 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw3G015400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2841 bp

ATGGTGAAGTCGTACCTCCGTTACGAGCAGGCGGCGGTCTTCGGGGTCATTTCCTCCGGCGATTCAAACATCACCTACGACAGCTCCGGCAAGCACCTTCTTGCTCCCGCCCTCGAAAAGGTAGGCGTTTGGCACGTACGACAGGGCGTTTGTACCAAAACCCTCACCCCTTCTTCCGCCACCGCCGGAAAACCCATCTCTGTCACCTCCATCGCTTCATCTCATACTTCTCTGATAGCTGCTGGGTATTCAGATGGTAGTATAAGAATTTGGGATTCTGAGAAAGGGACCTGTGAGACGACACTCAATGGACATAAAAAGGCTGTCACTGCCTTACGGTATGACAAGCTTGGATCTAAGCTTGCTTCTGGAAGCAAAGATGACGACATTATATTATGGGATGTGGTCGGTGAGAGCGGCCTATATCGACTCCGTGGGCATCGTGATCAGGTTACGGATCTTGTCTTCTTGGATTCTGGAAAGAAACTTGTTAGTTCCTCAAAAGATAAATTTCTGAAAGTATGGGATCTTGATACCCAGCATTGCATGCAGACAATTAGTGGCCACCATAGTGAAATCTGGTCTATAGATAGTGATCCAGAAGAAAGATATTTAGTCTCTGGGTCTGCAGACGCGGAGCTTAGATTTTACACAATTAAGCATGACTCGGAAGATGGCCAATCCATCTCTAATGGGAATGGAACTGTAACTGCGGGGAGTGGAGATCCACCGGTTCATGCTAAATGGGAAGTTTTGCAGATGTTTGGTGAGTTACCACGGAAAAACAACGACGGTAGAGTCGCAACTGTGAGATTCAACAAGTCTGGAAATTTGCTGGCTTGTCAGGTAGCGGGAAAGACAGTAGATATATTCCATGTACTGGATGAGGATGAATCAAAGAGGAAAGCAAAACGCAGGCTTCACCGGAAGAAAGAAAAAAAATCTGCCAAGGGGGCACCTCAGGTGTTAGAAAATGGAGACACAGATCGTGTAGTTGGAGAAGAAGGAAATAGTCCTGCTGTTACAGTCCTTGATGTTTTTAAGCTTCTTCAAACTATTCGAGCAAGCAAAAAGATATGTTCCATCTCGTTCTGTCCAAACACTACAAAGAGTTCGATGGCTACACTAGCTTTGTCATTGAACAATAATTTGTTGGAATTTTATTCTATCGAAAGCAATGCAACGAACAAAACACTTTCTGTAGAGCTTCAAGGACACCGATCTGATGTCAGAAGTGTAGCACTTAGCTCAGACAACACACTCTTGATGTCAACTAGTCACAATGCAGTAAAGATTTGGAATCCAAGTACTGGTTCCTGCCTGCGAACTATTGATTCTGGATATGGCATGTGTGGTTTTATCATTCCTAACAGCGAGTATGCACTTGTTGGAACTAAAAGTGGAACAATGGAAATTATTAACATTGGAAGTGGCACTTGCATTGAAGCAGTGGAAGCACACGGTGGCTCTGTCATGTCAATTGCTGCCATTCCTAATGAAAATGGTTTTGTCACAGGAAGTTCAGATCATGATGTTAAGTTTTGGGAATATGAAGTTAAACAGAAGTCTGCTCAAGATTCCAAGCAGCTAGTGGTGTCAAATGTAAGGACTATGAAGATGAACGACGATGCCCTTGTGGTTGCTGTCAGCCCTGATGCCAAATATATCTTGGTTGCACTATTGGATTGCACAGTGAAGGTGTTCTTTATGGATTCACTTAAGCTTTTCCTTTCCTTGTATGGTCATAAGTTGCCTGTATTATGTATGGATACATCATCTGACGGAGACCTAATAGTGACTGGATCTGCTGACAAAAATTTGAAGATTTGGGGTTTAGATTTTGGTGATTGTCATAAATCCTTATTCGCTCATGAGGACAGTGTAACAGCTGTTCGATTTGTGCAAAACACACATTACATGTTTAGTGCTGGAAAGGACCGCATTGTAAAATATTGGGATGCTGATAAATTTCAGTTGCTTTTAACGCTTGAGGGACACCATGCTGAAGTTTGGTGCCTCGGAATTAGTAACCGTGGGGACTTCATTGTCACGGGATCTCATGACAGATCAATACGTCGCTGGGATCGCACTGAAGAGCCATTTTTTATTGAGGAAGAAGAAGAAAAAAGGCAGTTGGAAATGATTGAGTCTGAACTTGACAATATGATAGAGAGCAGGTATCAAAAGGAAGAAATCCCTGAGGTGGGAGCTGTGGCGTTAGCAGGGAAGAAATCTAAAGAAACAATTACAGCTGCTGATGCAATTATCGATGCAATAGATATGGCAGACGCAGAATTGAAGCGAGTTGCGGAAGATGAGGAGGAGAAAAGCAGGGGCAATGTTGCTGACTTTAAACCAAATAGTCTCATGAATGGGCTCTCTCCATCTGAATATGTTCTTCGCGCATTTTCAAATGTTCAGACAAATGATCTTGAGCAGACGCTACTGGCTTTACCGTTCTCAGATGCTTTGAAGCTTATGTCTTACTTGAAGGACTGGACTTTGAATCCTGATAAGGTTGAACTCGTTTGCAAGATTTCTTTAGTTCTATTGCAGACACATTTTAATCAGTTGGTTAGTACACCGGCAGCAAGACCAATGTTAACTGCTTTGAAAGAGATTCTTTATCCCGAAGTTAAGGAACGGAAAGACATCATTGGTTTCAACCTTGCAGGGTTGGAACATCTTAAGCAACTAATGGCTTTGAGATCAGATGCATTATTTCAAGATGCAAAATCAAAGTTGCAGGTAATTCGTGCACAGCATTCCAAGCGCCTAGATGCAAGGACAGGCACAGGCAGAGAAGAAAATCAAAGTAAGAAGAGAAAGACAAAACAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005654 GO:0005730 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0012505 GO:0016020 GO:0016070 GO:0016072 GO:0022613 GO:0030490 GO:0030515 GO:0030684 GO:0030686 GO:0031090 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032040 GO:0032991 GO:0034388 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042274 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

Utp12 WD40

Protein Analysis

946

Amino Acids

104.53

Weight (kDa)

6.21

Isoelectric Point (pI)

39.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR75_1st	PF23869	10 - 136	4.5e-06	WD repeat-containing protein 75 first beta-propeller
Beta-prop_THOC3	PF25174	24 - 218	2.3e-29	THOC3 beta-propeller domain
WD40_CDC20-Fz	PF24807	27 - 222	3e-24	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	28 - 102	4.9e-07	WDR5 beta-propeller domain
WD40_MABP1-WDR62_2nd	PF24782	28 - 134	1.8e-07	MABP1/WDR62 second WD40 domain
Beta-prop_WDR36-Utp21_1st	PF25171	30 - 217	3.8e-14	WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st	PF25173	31 - 219	2.8e-74	WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	39 - 133	7.4e-06	WDR3 second beta-propeller domain
Beta-prop_EML	PF23409	40 - 135	1e-07	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_TEP1_2nd	PF25047	41 - 217	4.5e-15	TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	41 - 182	1.4e-11	WDR36/Utp21 second beta-propeller domain
WD40_Gbeta	PF25391	43 - 137	2.5e-06	G protein beta WD-40 repeat protein
Beta-prop_SCAP	PF24017	61 - 180	3.3e-14	SCAP Beta-propeller
Beta-prop_EML_2	PF23414	62 - 183	8.8e-13	Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st	PF24817	67 - 137	1.8e-09	WDHD1 first WD40 domain
Beta-prop_WDR3_2nd	PF25172	68 - 176	1.7e-14	WDR3 second beta-propeller domain
WDR90_beta-prop_4th	PF23342	69 - 128	1.5e-06	WDR90, 4th beta-propeller
Beta-prop_CAF1B_HIR1	PF24105	74 - 221	1e-13	CAF1B/HIR1 beta-propeller domain
WDR55	PF24796	75 - 228	3e-15	WDR55
Beta-prop_IFT140_1st	PF23383	76 - 135	7.7e-06	IFT140 first beta-propeller
WD40_Prp19	PF24814	77 - 134	2.3e-09	Prp19 WD40 domain
Beta-prop_EML_2	PF23414	78 - 221	1.2e-14	Echinoderm microtubule-associated protein second beta-propeller
WD40	PF00400	96 - 134	7e-07	WD domain, G-beta repeat
Beta-prop_WDR5	PF25175	101 - 221	3.1e-25	WDR5 beta-propeller domain
EIF3I	PF24805	101 - 183	1.8e-07	EIF3I
Beta-prop_Aladin	PF25460	106 - 227	6.7e-06	Aladin seven-bladed propeller
WD40_MABP1-WDR62_2nd	PF24782	118 - 224	2.9e-06	MABP1/WDR62 second WD40 domain
WD40_Prp19	PF24814	119 - 222	2.9e-18	Prp19 WD40 domain
WD40_Gbeta	PF25391	124 - 224	9.7e-10	G protein beta WD-40 repeat protein
WD40	PF00400	141 - 176	1.1e-06	WD domain, G-beta repeat
EIF3I	PF24805	144 - 221	2.2e-10	EIF3I
Beta-prop_WDR3_2nd	PF25172	144 - 218	1.7e-13	WDR3 second beta-propeller domain
WD40_WDHD1_1st	PF24817	150 - 224	3.7e-10	WDHD1 first WD40 domain
Beta-prop_WDR3_2nd	PF25172	172 - 227	1.9e-07	WDR3 second beta-propeller domain
WD40	PF00400	181 - 217	1e-05	WD domain, G-beta repeat
WD40_CDC20-Fz	PF24807	262 - 515	7.9e-16	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	264 - 533	3.7e-16	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	358 - 516	2.6e-14	WDR3 first beta-propeller domain
Beta-prop_THOC3	PF25174	376 - 619	1.6e-24	THOC3 beta-propeller domain
WD40_Gbeta	PF25391	378 - 610	6.7e-14	G protein beta WD-40 repeat protein
WD40_MABP1-WDR62_2nd	PF24782	405 - 652	2.1e-10	MABP1/WDR62 second WD40 domain
Beta-prop_WDR3_2nd	PF25172	409 - 700	2.1e-135	WDR3 second beta-propeller domain
PEP5_VPS11_N	PF23341	456 - 725	3.5e-07	PEP5/VPS11 N-terminal
WD40_WDHD1_1st	PF24817	457 - 572	3.6e-06	WDHD1 first WD40 domain
WD40_Prp19	PF24814	458 - 654	2.1e-22	Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	459 - 640	7.6e-13	WDR36/Utp21 second beta-propeller domain
WDR55	PF24796	460 - 612	4.1e-06	WDR55
Beta-prop_EML_2	PF23414	493 - 658	1.7e-13	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd	PF25047	500 - 697	5.9e-16	TEP-1 second beta-propeller
Beta-prop_THOC3	PF25174	534 - 695	3.5e-23	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	540 - 695	1.5e-31	WDR3 first beta-propeller domain
WD40_Gbeta	PF25391	541 - 694	7.2e-10	G protein beta WD-40 repeat protein
Beta-prop_WDR5	PF25175	546 - 697	6.1e-29	WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	556 - 694	2.5e-06	WDR36/Utp21 first beta-propeller
WD40	PF00400	577 - 610	1e-07	WD domain, G-beta repeat
Beta-prop_EML	PF23409	585 - 656	5.3e-06	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	586 - 695	1.6e-07	WDR36/Utp21 second beta-propeller domain
WD40	PF00400	615 - 653	7.9e-06	WD domain, G-beta repeat
WDR55	PF24796	620 - 708	6.7e-06	WDR55
EIF3I	PF24805	621 - 698	3e-10	EIF3I
WD40_Prp19	PF24814	636 - 802	6.1e-10	Prp19 WD40 domain
WD40	PF00400	658 - 695	2.8e-08	WD domain, G-beta repeat
Utp12	PF04003	796 - 898	4.5e-23	Dip2/Utp12 Family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

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Orthologous Genes (Group: OG0001070)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G21540
fragaria_vesca	FvH4_6g16900 FvH4_6g16900 FvH4_6g16900 FvH4_6g16900 FvH4_6g16900
malus_domestica	MD12G1106200.v1.1
prunus_persica	Prupe.7G125100_v2.0.a1
pyrus_communis	pycom12g10640
rosa_chinensis	RchiOBHm_Chr3g0470921 RchiOBHm_Chr7g0191891 RchiOBHm_Chr7g0191911 RchiOBHm_Chr7g0224541
rosa_laevigata	RLG00000001887 RLG00000004419 RLG00000004420 RLG00000017852 RLG00000024233
rosa_multiflora	Rmu_co8045794.1_g000001 Rmu_co8107512.1_g000001 Rmu_co8259631.1_g000001 Rmu_co8279299.1_g000001 Rmu_co8395733.1_g000001 Rmu_sc0003361.1_g000007 Rmu_sc0003527.1_g000004 Rmu_sc0005264.1_g000005 Rmu_sc0023809.1_g000001 Rmu_sc0037201.1_g000001 Rmu_sc0037202.1_g000001 Rmu_sc0038402.1_g000001 Rmu_sc0038402.1_g000003 Rmu_sc0039860.1_g000001 Rmu_ssc0000421.1_g000010
rosa_roxburghii	Rroxscaffold_3G00234650 Rroxscaffold_3G00263480 Rroxscaffold_3G00263490 Rroxscaffold_6G00410410
rosa_rugosa	Rorug03G0113900 Rorug06G0511700 Rorug06G0511700 Rorug07G0172700 Rorug07G0172800 Rorug07G0182600
rosa_samantha	Rh2CG630200 Rh2DG680600 Rh3AG163600 Rh3BG189100 Rh3CG179100 Rh3DG187100 Rh5AG328900 Rh7AG121800 Rh7AG378900 Rh7BG123300 Rh7BG123400 Rh7CG398600 Rh7DG124900 Rh7DG369600
rosa_wichuraiana	Rw3G015400 Rw7G010370 Rw7G031220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	797, 2045
Acc36I	ACCTGC	2 cut(s)	2166, 2737
AccB1I	GGYRCC	2 cut(s)	955, 2013
AccB7I	CCANNNNNTGG	2 cut(s)	880, 1310
AccBSI	CCGCTC	1 cut(s)	417
AccII	CGCG	2 cut(s)	635, 2403
AclWI	GGATC	8 cut(s)	361, 465, 534, 590, 719, 1810, 2062, 2091
AcoI	YGGCCR	2 cut(s)	562, 676
AcsI	RAATTY	6 cut(s)	267, 509, 829, 1157, 1816, 1967
AcuI	CTGAAG	2 cut(s)	2025, 2112
AdeI	CACNNNGTG	1 cut(s)	1463
AfaI	GTAC	6 cut(s)	14, 138, 154, 879, 1309, 2581
AfiI	CCNNNNNNNGG	5 cut(s)	118, 880, 1310, 2203, 2204
AflII	CTTAAG	2 cut(s)	1718, 2687
AflIII	ACRYGT	1 cut(s)	1917
AgeI	ACCGGT	1 cut(s)	730
AhlI	ACTAGT	1 cut(s)	1274
AjuI	GAANNNNNNNTTGG	2 cut(s)	1297, 1329
AleI	CACNNNNGTG	1 cut(s)	570
Alw21I	GWGCWC	1 cut(s)	2761
Alw26I	GTCTC	5 cut(s)	290, 622, 972, 1779, 2369
Alw44I	GTGCAC	1 cut(s)	2757
AlwI	GGATC	8 cut(s)	361, 465, 534, 590, 719, 1810, 2062, 2091
AlwNI	CAGNNNCTG	1 cut(s)	2255
AoxI	GGCC	3 cut(s)	418, 562, 676
ApaLI	GTGCAC	1 cut(s)	2757
ApeKI	GCWGC	5 cut(s)	239, 1484, 1585, 2252, 2588
ApoI	RAATTY	6 cut(s)	267, 509, 829, 1157, 1816, 1967
ArsI	GACNNNNNNTTYG	2 cut(s)	132, 164
AsiGI	ACCGGT	1 cut(s)	730
Asp700I	GAANNNNTTC	2 cut(s)	1700, 2394
AspLEI	GCGC	2 cut(s)	2405, 2775
AspS9I	GGNCC	2 cut(s)	288, 1936
AsuHPI	GGTGA	8 cut(s)	16, 64, 157, 196, 422, 779, 914, 1856
AvaII	GGWCC	2 cut(s)	288, 1936
AxyI	CCTNAGG	2 cut(s)	960, 2199
BaeGI	GKGCMC	2 cut(s)	958, 2761
BalI	TGGCCA	2 cut(s)	564, 678
BanI	GGYRCC	2 cut(s)	955, 2013
BanII	GRGCYC	1 cut(s)	2379
BbsI	GAAGAC	2 cut(s)	31, 457
Bbv12I	GWGCWC	1 cut(s)	2761
BbvI	GCAGC	5 cut(s)	226, 1471, 1597, 2239, 2600
BccI	CCATC	8 cut(s)	203, 218, 248, 668, 692, 1091, 1111, 2392
BcgI	CGANNNNNNTGC	4 cut(s)	1160, 1194, 2735, 2769
BciVI	GTATCC	1 cut(s)	1762
BclI	TGATCA	1 cut(s)	445
BcoDI	GTCTC	5 cut(s)	290, 622, 972, 1779, 2369
BcuI	ACTAGT	1 cut(s)	1274
BfaI	CTAG	4 cut(s)	1127, 1275, 1589, 2777
BfmI	CTRYAG	3 cut(s)	585, 627, 1200
BfoI	RGCGCY	1 cut(s)	2776
BfrI	CTTAAG	2 cut(s)	1718, 2687
BfuAI	ACCTGC	2 cut(s)	2166, 2737
BfuI	GTATCC	1 cut(s)	1762
BglI	GCCNNNNNGGC	1 cut(s)	953
BisI	GCNGC	7 cut(s)	33, 240, 418, 1485, 1586, 2253, 2589
BlsI	GCNGC	7 cut(s)	34, 241, 419, 1486, 1587, 2254, 2590
BmcAI	AGTACT	1 cut(s)	1309
Bme18I	GGWCC	2 cut(s)	288, 1936
BmgT120I	GGNCC	2 cut(s)	288, 1936
BmiI	GGNNCC	4 cut(s)	289, 957, 1315, 2015
BpiI	GAAGAC	2 cut(s)	31, 457
BpuEI	CTTGAG	3 cut(s)	1557, 2009, 2453
Bsa29I	ATCGAT	1 cut(s)	2268
BsaAI	YACGTR	1 cut(s)	136
BsaBI	GATNNNNATC	1 cut(s)	594
BsaI	GGTCTC	1 cut(s)	1779
BsaJI	CCNNGG	5 cut(s)	433, 776, 948, 2017, 2032
BsaWI	WCCGGW	2 cut(s)	730, 924
BsaXI	ACNNNNNCTCC	2 cut(s)	68, 98
Bsc4I	CCNNNNNNNGG	5 cut(s)	118, 880, 1310, 2203, 2204
Bse118I	RCCGGY	2 cut(s)	730, 2584
Bse1I	ACTGG	5 cut(s)	885, 1315, 1804, 2451, 2501
Bse21I	CCTNAGG	2 cut(s)	960, 2199
Bse3DI	GCAATG	3 cut(s)	541, 1183, 2344
Bse8I	GATNNNNATC	1 cut(s)	594
BseCI	ATCGAT	1 cut(s)	2268
BseDI	CCNNGG	5 cut(s)	433, 776, 948, 2017, 2032
BseGI	GGATG	4 cut(s)	406, 889, 895, 1963
BseJI	GATNNNNATC	1 cut(s)	594
BseLI	CCNNNNNNNGG	5 cut(s)	118, 880, 1310, 2203, 2204
BseMI	GCAATG	3 cut(s)	541, 1183, 2344
BseMII	CTCAG	5 cut(s)	270, 974, 1263, 2190, 2475
BseNI	ACTGG	5 cut(s)	885, 1315, 1804, 2451, 2501
BseRI	GAGGAG	2 cut(s)	2333, 2336
BseSI	GKGCMC	2 cut(s)	958, 2761
BseXI	GCAGC	5 cut(s)	226, 1471, 1597, 2239, 2600
BseYI	CCCAGC	3 cut(s)	242, 537, 2079
Bsh1236I	CGCG	2 cut(s)	635, 2403
BshFI	GGCC	3 cut(s)	420, 564, 678
BshNI	GGYRCC	2 cut(s)	955, 2013
BshTI	ACCGGT	1 cut(s)	730
BshVI	ATCGAT	1 cut(s)	2268
BsiHKAI	GWGCWC	1 cut(s)	2761
BsiSI	CCGG	6 cut(s)	57, 87, 186, 731, 925, 2585
BsiWI	CGTACG	1 cut(s)	136
BslFI	GGGAC	3 cut(s)	301, 2007, 2051
BslI	CCNNNNNNNGG	5 cut(s)	118, 880, 1310, 2203, 2204
BsmAI	GTCTC	5 cut(s)	290, 622, 972, 1779, 2369
BsmBI	CGTCTC	1 cut(s)	290
BsmFI	GGGAC	3 cut(s)	301, 2007, 2051
BsmI	GAATGC	1 cut(s)	2764
BsnI	GGCC	3 cut(s)	420, 564, 678
Bso31I	GGTCTC	1 cut(s)	1779
Bsp1286I	GDGCHC	3 cut(s)	958, 2379, 2761
BspANI	GGCC	3 cut(s)	420, 564, 678
BspCNI	CTCAG	5 cut(s)	271, 973, 1262, 2191, 2474
BspDI	ATCGAT	1 cut(s)	2268
BspFNI	CGCG	2 cut(s)	635, 2403
BspHI	TCATGA	4 cut(s)	1528, 1870, 2059, 2367
BspLI	GGNNCC	4 cut(s)	289, 957, 1315, 2015
BspMAI	CTGCAG	1 cut(s)	631
BspMI	ACCTGC	2 cut(s)	2166, 2737
BspPI	GGATC	8 cut(s)	361, 465, 534, 590, 719, 1810, 2062, 2091
BspQI	GCTCTTC	1 cut(s)	2088
BspT107I	GGYRCC	2 cut(s)	955, 2013
BspTI	CTTAAG	2 cut(s)	1718, 2687
BspTNI	GGTCTC	1 cut(s)	1779
BsrBI	CCGCTC	1 cut(s)	417
BsrDI	GCAATG	3 cut(s)	541, 1183, 2344
BsrFI	RCCGGY	2 cut(s)	730, 2584
BsrI	ACTGG	5 cut(s)	885, 1315, 1804, 2451, 2501
BssAI	RCCGGY	2 cut(s)	730, 2584
BssECI	CCNNGG	5 cut(s)	433, 776, 948, 2017, 2032
BssT1I	CCWWGG	1 cut(s)	948
Bst6I	CTCTTC	2 cut(s)	2088, 2816
BstAFI	CTTAAG	2 cut(s)	1718, 2687
BstAPI	GCANNNNNTGC	1 cut(s)	547
BstBAI	YACGTR	1 cut(s)	136
BstC8I	GCNNGC	8 cut(s)	30, 92, 363, 548, 837, 917, 1066, 1322
BstDEI	CTNAG	8 cut(s)	279, 357, 641, 960, 1244, 1249, 2199, 2461
BstDSI	CCRYGG	3 cut(s)	433, 776, 2032
BstENI	CCTNNNNNAGG	1 cut(s)	116
BstF5I	GGATG	4 cut(s)	406, 889, 895, 1963
BstFNI	CGCG	2 cut(s)	635, 2403
BstH2I	RGCGCY	1 cut(s)	2776
BstHHI	GCGC	2 cut(s)	2405, 2775
BstMAI	GTCTC	5 cut(s)	290, 622, 972, 1779, 2369
BstMWI	GCNNNNNNNGC	9 cut(s)	329, 547, 953, 1127, 1454, 1984, 2085, 2258, 2770
BstNSI	RCATGY	3 cut(s)	550, 1351, 1921
BstSFI	CTRYAG	3 cut(s)	585, 627, 1200
BstSLI	GKGCMC	2 cut(s)	958, 2761
BstUI	CGCG	2 cut(s)	635, 2403
BstV1I	GCAGC	5 cut(s)	226, 1471, 1597, 2239, 2600
BstV2I	GAAGAC	2 cut(s)	31, 457
BstX2I	RGATCY	6 cut(s)	353, 457, 526, 724, 1802, 2054
BstYI	RGATCY	6 cut(s)	353, 457, 526, 724, 1802, 2054
Bsu15I	ATCGAT	1 cut(s)	2268
Bsu36I	CCTNAGG	2 cut(s)	960, 2199
BsuI	GTATCC	1 cut(s)	1762
BsuRI	GGCC	3 cut(s)	420, 564, 678
BsuTUI	ATCGAT	1 cut(s)	2268
BtgI	CCRYGG	3 cut(s)	433, 776, 2032
BtgZI	GCGATG	1 cut(s)	196
BtsCI	GGATG	4 cut(s)	406, 889, 895, 1963
BtsI	GCAGTG	2 cut(s)	327, 1455
BtsIMutI	CAGTG	5 cut(s)	327, 1455, 1698, 1885, 2088
BveI	ACCTGC	2 cut(s)	2166, 2737
Cac8I	GCNNGC	8 cut(s)	30, 92, 363, 548, 837, 917, 1066, 1322
CaiI	CAGNNNCTG	1 cut(s)	2255
CciI	TCATGA	4 cut(s)	1528, 1870, 2059, 2367
CfoI	GCGC	2 cut(s)	2405, 2775
Cfr10I	RCCGGY	2 cut(s)	730, 2584
Cfr13I	GGNCC	2 cut(s)	288, 1936
ClaI	ATCGAT	1 cut(s)	2268
CseI	GACGC	3 cut(s)	641, 2297, 2449
Csp6I	GTAC	6 cut(s)	13, 137, 153, 878, 1308, 2580
CspAI	ACCGGT	1 cut(s)	730
CviQI	GTAC	6 cut(s)	13, 137, 153, 878, 1308, 2580
DdeI	CTNAG	8 cut(s)	279, 357, 641, 960, 1244, 1249, 2199, 2461
DraI	TTTAAA	1 cut(s)	2353
DraIII	CACNNNGTG	1 cut(s)	1463
DrdI	GACNNNNNNGTC	2 cut(s)	797, 2045
DseDI	GACNNNNNNGTC	2 cut(s)	797, 2045
EaeI	YGGCCR	2 cut(s)	562, 676
Eam1104I	CTCTTC	2 cut(s)	2088, 2816
EarI	CTCTTC	2 cut(s)	2088, 2816
EciI	GGCGGA	1 cut(s)	166
Eco130I	CCWWGG	1 cut(s)	948
Eco24I	GRGCYC	1 cut(s)	2379
Eco31I	GGTCTC	1 cut(s)	1779
Eco47I	GGWCC	2 cut(s)	288, 1936
Eco57I	CTGAAG	2 cut(s)	2025, 2112
Eco81I	CCTNAGG	2 cut(s)	960, 2199
EcoNI	CCTNNNNNAGG	1 cut(s)	116
EcoO109I	RGGNCCY	1 cut(s)	288
EcoT14I	CCWWGG	1 cut(s)	948
EcoT22I	ATGCAT	1 cut(s)	2719
EcoT38I	GRGCYC	1 cut(s)	2379
ErhI	CCWWGG	1 cut(s)	948
Esp3I	CGTCTC	1 cut(s)	290
FalI	AAGNNNNNCTT	6 cut(s)	1420, 1452, 1688, 1720, 2607, 2639
FaqI	GGGAC	3 cut(s)	301, 2007, 2051
FauI	CCCGC	3 cut(s)	115, 706, 844
FbaI	TGATCA	1 cut(s)	445
Fnu4HI	GCNGC	7 cut(s)	33, 240, 418, 1485, 1586, 2253, 2589
FokI	GGATG	4 cut(s)	413, 896, 902, 1970
FriOI	GRGCYC	1 cut(s)	2379
Fsp4HI	GCNGC	7 cut(s)	33, 240, 418, 1485, 1586, 2253, 2589
FspBI	CTAG	4 cut(s)	1127, 1275, 1589, 2777
GlaI	GCGC	2 cut(s)	2404, 2774
GluI	GCNGC	7 cut(s)	33, 240, 418, 1485, 1586, 2253, 2589
GsaI	CCCAGC	3 cut(s)	246, 541, 2083
HaeII	RGCGCY	1 cut(s)	2776
HaeIII	GGCC	3 cut(s)	420, 564, 678
HapII	CCGG	6 cut(s)	57, 87, 186, 731, 925, 2585
HgaI	GACGC	3 cut(s)	641, 2297, 2449
HhaI	GCGC	2 cut(s)	2405, 2775
Hin6I	GCGC	2 cut(s)	2403, 2773
HinP1I	GCGC	2 cut(s)	2403, 2773
HincII	GTYRAC	2 cut(s)	1272, 2604
HindII	GTYRAC	2 cut(s)	1272, 2604
HindIII	AAGCTT	5 cut(s)	347, 359, 1043, 1721, 2474
HpaI	GTTAAC	1 cut(s)	2604
HpaII	CCGG	6 cut(s)	57, 87, 186, 731, 925, 2585
HphI	GGTGA	8 cut(s)	16, 64, 157, 196, 422, 779, 914, 1856
Hpy166II	GTNNAC	4 cut(s)	1272, 2582, 2604, 2759
Hpy8I	GTNNAC	4 cut(s)	1272, 2582, 2604, 2759
Hpy99I	CGWCG	3 cut(s)	794, 1628, 2079
HpyAV	CCTTC	5 cut(s)	107, 180, 998, 1690, 2485
HpyCH4IV	ACGT	2 cut(s)	135, 2074
HpyF10VI	GCNNNNNNNGC	9 cut(s)	329, 547, 953, 1127, 1454, 1984, 2085, 2258, 2770
HpyF3I	CTNAG	8 cut(s)	279, 357, 641, 960, 1244, 1249, 2199, 2461
HpySE526I	ACGT	2 cut(s)	135, 2074
HspAI	GCGC	2 cut(s)	2403, 2773
Ksp22I	TGATCA	1 cut(s)	445
KspAI	GTTAAC	1 cut(s)	2604
LguI	GCTCTTC	1 cut(s)	2088
LmnI	GCTCC	4 cut(s)	89, 109, 637, 2207
Lsp1109I	GCAGC	5 cut(s)	226, 1471, 1597, 2239, 2600
MaeI	CTAG	4 cut(s)	1127, 1275, 1589, 2777
MaeII	ACGT	2 cut(s)	135, 2074
MbiI	CCGCTC	1 cut(s)	417
MfeI	CAATTG	1 cut(s)	1479
MflI	RGATCY	6 cut(s)	353, 457, 526, 724, 1802, 2054
MhlI	GDGCHC	3 cut(s)	958, 2379, 2761
MlsI	TGGCCA	2 cut(s)	564, 678
MluNI	TGGCCA	2 cut(s)	564, 678
MlyI	GAGTC	4 cut(s)	423, 659, 807, 2156
MmeI	TCCRAC	5 cut(s)	976, 1134, 1369, 2115, 2658
Mox20I	TGGCCA	2 cut(s)	564, 678
Mph1103I	ATGCAT	1 cut(s)	2719
MroXI	GAANNNNTTC	2 cut(s)	1700, 2394
MscI	TGGCCA	2 cut(s)	564, 678
MslI	CAYNNNNRTG	1 cut(s)	570
Msp20I	TGGCCA	2 cut(s)	564, 678
MspA1I	CMGCKG	2 cut(s)	1889, 2252
MspCI	CTTAAG	2 cut(s)	1718, 2687
MspI	CCGG	6 cut(s)	57, 87, 186, 731, 925, 2585
MunI	CAATTG	1 cut(s)	1479
Mva1269I	GAATGC	1 cut(s)	2764
MvnI	CGCG	2 cut(s)	635, 2403
MwoI	GCNNNNNNNGC	9 cut(s)	329, 547, 953, 1127, 1454, 1984, 2085, 2258, 2770
NlaIV	GGNNCC	4 cut(s)	289, 957, 1315, 2015
NmuCI	GTSAC	6 cut(s)	202, 325, 1277, 1510, 1795, 2047
NsiI	ATGCAT	1 cut(s)	2719
NspI	RCATGY	3 cut(s)	550, 1351, 1921
OliI	CACNNNNGTG	1 cut(s)	570
PaeI	GCATGC	1 cut(s)	550
PagI	TCATGA	4 cut(s)	1528, 1870, 2059, 2367
PciI	ACATGT	1 cut(s)	1917
PciSI	GCTCTTC	1 cut(s)	2088
PctI	GAATGC	1 cut(s)	2764
PdmI	GAANNNNTTC	2 cut(s)	1700, 2394
Pfl23II	CGTACG	1 cut(s)	136
PflMI	CCANNNNNTGG	2 cut(s)	880, 1310
PinAI	ACCGGT	1 cut(s)	730
PkrI	GCNGC	7 cut(s)	34, 241, 419, 1486, 1587, 2254, 2590
PleI	GAGTC	4 cut(s)	423, 659, 806, 2155
PpsI	GAGTC	4 cut(s)	423, 659, 806, 2155
Ppu21I	YACGTR	1 cut(s)	136
PpuMI	RGGWCCY	1 cut(s)	288
PscI	ACATGT	1 cut(s)	1917
Psp5II	RGGWCCY	1 cut(s)	288
PspFI	CCCAGC	3 cut(s)	242, 537, 2079
PspLI	CGTACG	1 cut(s)	136
PspN4I	GGNNCC	4 cut(s)	289, 957, 1315, 2015
PspPI	GGNCC	2 cut(s)	288, 1936
PspPPI	RGGWCCY	1 cut(s)	288
PsrI	GAACNNNNNNTAC	2 cut(s)	1875, 1907
PstI	CTGCAG	1 cut(s)	631
PstNI	CAGNNNCTG	1 cut(s)	2255
PsuI	RGATCY	6 cut(s)	353, 457, 526, 724, 1802, 2054
PvuII	CAGCTG	2 cut(s)	1889, 2252
RsaI	GTAC	6 cut(s)	14, 138, 154, 879, 1309, 2581
RsaNI	GTAC	6 cut(s)	13, 137, 153, 878, 1308, 2580
RseI	CAYNNNNRTG	1 cut(s)	570
SapI	GCTCTTC	1 cut(s)	2088
SatI	GCNGC	7 cut(s)	33, 240, 418, 1485, 1586, 2253, 2589
Sau96I	GGNCC	2 cut(s)	288, 1936
ScaI	AGTACT	1 cut(s)	1309
SchI	GAGTC	4 cut(s)	423, 659, 807, 2156
SduI	GDGCHC	3 cut(s)	958, 2379, 2761
SfcI	CTRYAG	3 cut(s)	585, 627, 1200
SinI	GGWCC	2 cut(s)	288, 1936
SmiMI	CAYNNNNRTG	1 cut(s)	570
SmlI	CTYRAG	5 cut(s)	1572, 1718, 1988, 2432, 2687
SmoI	CTYRAG	5 cut(s)	1572, 1718, 1988, 2432, 2687
SpeI	ACTAGT	1 cut(s)	1274
SphI	GCATGC	1 cut(s)	550
SspI	AATATT	1 cut(s)	1952
SspMI	CTAG	4 cut(s)	1127, 1275, 1589, 2777
StyI	CCWWGG	1 cut(s)	948
TaiI	ACGT	2 cut(s)	138, 2077
TaqI	TCGA	7 cut(s)	114, 427, 1060, 1115, 1170, 1894, 2268
TatI	WGTACW	3 cut(s)	877, 1307, 2579
TauI	GCSGC	2 cut(s)	35, 420
TscAI	CASTG	5 cut(s)	334, 1455, 1698, 1885, 2095
TseFI	GTSAC	6 cut(s)	202, 325, 1277, 1510, 1795, 2047
TseI	GCWGC	5 cut(s)	239, 1484, 1585, 2252, 2588
Tsp45I	GTSAC	6 cut(s)	202, 325, 1277, 1510, 1795, 2047
TspDTI	ATGAA	9 cut(s)	207, 725, 906, 1512, 1566, 1628, 1634, 2032, 2384
TspGWI	ACGGA	6 cut(s)	8, 422, 470, 793, 1797, 2659
TspRI	CASTG	5 cut(s)	334, 1455, 1698, 1885, 2095
Van91I	CCANNNNNTGG	2 cut(s)	880, 1310
Vha464I	CTTAAG	2 cut(s)	1718, 2687
VneI	GTGCAC	1 cut(s)	2757
VpaK11BI	GGWCC	2 cut(s)	288, 1936
XagI	CCTNNNNNAGG	1 cut(s)	116
XapI	RAATTY	6 cut(s)	267, 509, 829, 1157, 1816, 1967
XceI	RCATGY	3 cut(s)	550, 1351, 1921
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1588, 1666
XmnI	GAANNNNTTC	2 cut(s)	1700, 2394
XspI	CTAG	4 cut(s)	1127, 1275, 1589, 2777
ZrmI	AGTACT	1 cut(s)	1309
Zsp2I	ATGCAT	1 cut(s)	2719

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.