Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0001070

Physical Location & Seq

Genomic Coordinates Forward (+)

21240900 .. 21247924

Gene Structure 7025 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00234650.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2673 bp

ATGGTGAAGTCGTACTTGCGATACGAGCAGGGTGCCGCCTTCGGCGTGATAGTTTCTGGCAATTCAAACATCGCCTACGACAGTTCCGGGAAGCACATTTTTGCCCCCGGCCTGGAAAAGGTGGGCGTGTGGAACGTGCGACAAGGCATTTGCATCAAAACCTTAACTCCTTGTGCTGCTGATGGATATTCGCCGCCAGTCACCTCCATCGCGGTCTCGCCTTCTTCTTCTTCTCTGATAGCCACTGGGTATGGAGATGGGAGCATTAGAATTTGGGACTCTCAGAATAAAATTTGTGAGACCACATTCTACGGTCATACGGGGGCCGTTAGTGTCTTACGGTACAACAAGATTGGAGATAGGCTGGTTTCTGGTGGCCAAGATAAGGACATTATATTGTGGGATGTGGAGAGGGGCGAGGGCATCTATCGCCTCCGCGGGCATGGGAATCAGACAATAAGTGACCACCATAGTGAAATTTGGTCCATAGATATCGATCCGGAAGAAAGATATTTGGTCTCTGGCTCTGCTGACTTGGAACTTAGGGTTTACACAATTAAGCATGACCTTAAAGCTTGCCAATCCACTTCTAATGCGAATGCAAATGGAACAGAAATTGCAGACTCTGGAGATCCATCCCCTCATAATAAATGGCAAGTTTTGAAGCTGTTTGGTGAATATCAGCGAAAAAGCAAGAACAGAGTTGCAACTGTGAGATTCAACAAGTCTGGAAATTTGCTGGCATGTCAAGAGGCAGGAAAGGCGGTACATATATTCAACGTACTGGATGAGGCTGAATCTACCCGTAAAGCAAACCGCAGGATTCGCCGGATCAAAAAGAAAAAGTCTGCCCAAGGGGAAGCCGAGGTGATGAAGGATGATGGAGCTGGAGAAGAGGGAAAGAGGCACGTGGTCATGGTCTCTGATGTTTTTCAACTTTTCCAAACTATCCGAACTGGCAAAAAGATATGTTCCATCTCTTTTTGTCCAAACCCTCCCAAGACTTCCCTAGCCACAATAGCTTTGTCTCTGAGCGATAATCTATGCGTGTTTTATTCTCTTGAGAGCGAAGCAACAAAACAAACACGCGCTATAGACCTCCACGGCCACCGATCATCTGTCACAAGTGTAGCGCTTAGCTCAGACAACTCAGTTTTGATGTCAACCAGTCATAATGCAGCAAAGATCTGGAATCCAACCAATGGTTACTGCCTTGGAACTATTGACACAGGATTCGCCATGTGTGCTTTTATCCTTCCTTCTTGCAATAACAAGTTTGCACTTGTTGGAACCAAGGGTGGAACAATGGAAATTATTGACGTTGGGAGTGCAACTGTCACTGAAGTAGTAGAAGCACATGGTGGCTCTGTTCAGTCTATTGCAAGAATTCCCAATGAAAATGGTTTTGTCACAGGAAGCTCAGATGGTTATGTTAAGTTCTGGGAATATGGAGTCAAAGAAGATTCTAAGCGCCTGCTAGTGTCAAATGTAAGAATGAAGGAGATGGATACTGATGTTCGTGTGGTTTCTATCAGTCCTGATGCCAAATATCTTTTGGTGGCGTTGGATACCATAGTAAAGGTATTCTATATGGATTCACTCAAACTTTTCGTCACCTTATATGGTCACAAGCTGCCAGTGCTATGTCTCGATGTCTCATCCGATGGAGAGTTGATAGTGACTGGATCTCAAGACAAAAATGTGAAAATTTGGGGGCTAGATTTTGGTGACTGCCACAAATCAATGTTTGATCATCAGAGCAGCGTTCAGCAAGTACAATTTGTGCCAAATACCCATTACTTTGTTAGTGTTGGGGATGATAAGCTAGTAAACTACTGGGATGCTGATAAATTTGAGTTGCTTTTAACTATGAAGGGACATCATGAGGGTGTTTGTTGCCTCGCTATTAGTAACCGTGGTGATTTCTTTGTCACGGGATCTCATGATCGCTCAATTCGCCGCTGGGATCGTACTGATGAGCCATTTTTCCTTGAGGAAGAGAGGGAAAAGCGGGAGTTGGAAAAGATGTACGAGTCTGACTTCAAGGATGAATATGAGAACAATTTTGCACTCAAGGAAAAGATCCCTGCAGAGGGAGCTGTGGCTTTTGCTGGGAAGAATACTAAAGAAACAATTTCTTTGACCGACTCAATTGTTGCTAAACTAGAAATAGCATGGGAAGAATCAAAGCGACGAGCCAATTATGAGGCGGAAAAAGTCAGTGGAAAGCTGGTTGCTGAGTTTCAACCAAATCCTTTTATGCTAGGGCTGTCTCCTCCTGATTATTTTCTGCATGAACTTTTGAAGGTTAAGACAAGCGATTTGGAGTCGATGCTATTGTTTCTTCCATTGTTAAGATTACTGACACTGGTAGTGCAGGGGCTACCCTTCTCGGATGCCTTGAAGATTTTGTCTTTCTGCAAGGATGTTGGTCTACGTCCTGATGAGGCAAAGAAGGATATCATTGGTTACAACCTTGCAGCAATGACTCATATCAAGCAATTGCATGATTCAAGATCAAATGCACCACTTCCAGATGCCATCTCCAAGCGTGGTGTAGGTGCAAGTTGGTATGGAAAGACAGAAGAAGGTAAATGCAAAAAAGTTCGAACATGTGAACCCGCGCCTGGCATGCATCGACACAGTTGGTTACAGCCCTGCAGCAAAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005654 GO:0005730 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0012505 GO:0016020 GO:0016070 GO:0016072 GO:0022613 GO:0030490 GO:0030515 GO:0030684 GO:0030686 GO:0031090 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032040 GO:0032991 GO:0034388 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042274 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

Utp12 WD40

Protein Analysis

890

Amino Acids

98.69

Weight (kDa)

6.94

Isoelectric Point (pI)

37.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_THOC3	PF25174	23 - 148	2e-14	THOC3 beta-propeller domain
Beta-prop_WDR5	PF25175	24 - 188	1.1e-20	WDR5 beta-propeller domain
WD40_CDC20-Fz	PF24807	29 - 196	1.4e-17	CDC20/Fizzy WD40 domain
Beta-prop_EML	PF23409	31 - 137	4.8e-07	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_1st	PF25173	32 - 151	1.5e-34	WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	41 - 185	6.1e-09	WDR36/Utp21 first beta-propeller
Beta-prop_EML_2	PF23414	41 - 194	2e-10	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd	PF25047	43 - 140	3e-07	TEP-1 second beta-propeller
Beta-prop_WDR75_1st	PF23869	55 - 137	3.7e-07	WD repeat-containing protein 75 first beta-propeller
WD40_Gbeta	PF25391	60 - 136	1.1e-06	G protein beta WD-40 repeat protein
Beta-prop_WDR3_2nd	PF25172	62 - 184	8e-13	WDR3 second beta-propeller domain
WD40_Prp19	PF24814	64 - 199	3.2e-17	Prp19 WD40 domain
Beta-prop_SPT8	PF23798	66 - 193	4.2e-06	SPT8 beta propeller
Beta-prop_SCAP	PF24017	75 - 150	8.4e-07	SCAP Beta-propeller
WD40_MABP1-WDR62_2nd	PF24782	76 - 204	5.5e-06	MABP1/WDR62 second WD40 domain
Beta-prop_IFT140_1st	PF23383	77 - 137	2.1e-06	IFT140 first beta-propeller
WD40_Gbeta	PF25391	80 - 191	1.3e-08	G protein beta WD-40 repeat protein
Beta-prop_CAF1B_HIR1	PF24105	99 - 198	1.5e-07	CAF1B/HIR1 beta-propeller domain
EIF3I	PF24805	104 - 210	2.4e-10	EIF3I
Beta-prop_WDR3_1st	PF25173	151 - 186	1.4e-06	WDR3 first beta-propeller domain
WD40_CDC20-Fz	PF24807	230 - 481	4.6e-12	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	321 - 521	4.5e-14	WDR5 beta-propeller domain
WD40_Gbeta	PF25391	340 - 571	4.5e-15	G protein beta WD-40 repeat protein
Beta-prop_THOC3	PF25174	344 - 599	1.5e-27	THOC3 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	374 - 661	2.6e-111	WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	416 - 609	6.9e-13	WDR36/Utp21 second beta-propeller domain
WD40_CDC20-Fz	PF24807	420 - 655	4.2e-22	CDC20/Fizzy WD40 domain
WD40_WDHD1_1st	PF24817	423 - 540	1.9e-07	WDHD1 first WD40 domain
WD40_Prp19	PF24814	423 - 614	1.8e-22	Prp19 WD40 domain
WDR55	PF24796	431 - 656	1.3e-15	WDR55
Beta-prop_EML_2	PF23414	459 - 594	3.6e-10	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd	PF25047	466 - 657	4.5e-15	TEP-1 second beta-propeller
WD40_MABP1-WDR62_2nd	PF24782	498 - 656	2.2e-10	MABP1/WDR62 second WD40 domain
Beta-prop_WDR3_1st	PF25173	499 - 656	2.2e-28	WDR3 first beta-propeller domain
WD40_WDHD1_1st	PF24817	502 - 662	5.9e-17	WDHD1 first WD40 domain
Beta-prop_WDR5	PF25175	503 - 658	3.6e-30	WDR5 beta-propeller domain
Beta-prop_IFT122_1st	PF23381	507 - 607	4.7e-07	IFT122 first beta-propeller
WD40_Gbeta	PF25391	518 - 657	3e-11	G protein beta WD-40 repeat protein
EIF3I	PF24805	520 - 602	8.9e-09	EIF3I
WD40_Prp19	PF24814	523 - 659	3.3e-20	Prp19 WD40 domain
WD40	PF00400	537 - 571	9.3e-09	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	538 - 616	9.1e-06	CAF1B/HIR1 beta-propeller domain
WD40_MABP1-WDR62_1st	PF24780	547 - 655	1.6e-09	MABP1/WDR62 first WD40 domain
Beta-prop_THOC3	PF25174	580 - 656	8.5e-12	THOC3 beta-propeller domain
EIF3I	PF24805	584 - 657	5.7e-06	EIF3I
WD40	PF00400	619 - 656	1.2e-08	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001070)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G21540
fragaria_vesca	FvH4_6g16900 FvH4_6g16900 FvH4_6g16900 FvH4_6g16900 FvH4_6g16900
malus_domestica	MD12G1106200.v1.1
prunus_persica	Prupe.7G125100_v2.0.a1
pyrus_communis	pycom12g10640
rosa_chinensis	RchiOBHm_Chr3g0470921 RchiOBHm_Chr7g0191891 RchiOBHm_Chr7g0191911 RchiOBHm_Chr7g0224541
rosa_laevigata	RLG00000001887 RLG00000004419 RLG00000004420 RLG00000017852 RLG00000024233
rosa_multiflora	Rmu_co8045794.1_g000001 Rmu_co8107512.1_g000001 Rmu_co8259631.1_g000001 Rmu_co8279299.1_g000001 Rmu_co8395733.1_g000001 Rmu_sc0003361.1_g000007 Rmu_sc0003527.1_g000004 Rmu_sc0005264.1_g000005 Rmu_sc0023809.1_g000001 Rmu_sc0037201.1_g000001 Rmu_sc0037202.1_g000001 Rmu_sc0038402.1_g000001 Rmu_sc0038402.1_g000003 Rmu_sc0039860.1_g000001 Rmu_ssc0000421.1_g000010
rosa_roxburghii	Rroxscaffold_3G00234650 Rroxscaffold_3G00263480 Rroxscaffold_3G00263490 Rroxscaffold_6G00410410
rosa_rugosa	Rorug03G0113900 Rorug06G0511700 Rorug06G0511700 Rorug07G0172700 Rorug07G0172800 Rorug07G0182600
rosa_samantha	Rh2CG630200 Rh2DG680600 Rh3AG163600 Rh3BG189100 Rh3CG179100 Rh3DG187100 Rh5AG328900 Rh7AG121800 Rh7AG378900 Rh7BG123300 Rh7BG123400 Rh7CG398600 Rh7DG124900 Rh7DG369600
rosa_wichuraiana	Rw3G015400 Rw7G010370 Rw7G031220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	32
AccB7I	CCANNNNNTGG	1 cut(s)	1202
AccI	GTMKAC	1 cut(s)	2434
AccII	CGCG	4 cut(s)	212, 438, 1089, 2624
AccIII	TCCGGA	1 cut(s)	499
AclWI	GGATC	7 cut(s)	491, 626, 839, 1693, 1945, 1974, 2077
AcoI	YGGCCR	2 cut(s)	376, 1105
AcsI	RAATTY	7 cut(s)	270, 291, 477, 733, 1386, 1707, 1850
AcuI	CTGAAG	1 cut(s)	1362
AcvI	CACGTG	1 cut(s)	910
AdeI	CACNNNGTG	1 cut(s)	1361
AfaI	GTAC	7 cut(s)	14, 344, 768, 783, 1776, 1972, 2030
AfeI	AGCGCT	1 cut(s)	1134
AfiI	CCNNNNNNNGG	7 cut(s)	112, 118, 385, 1202, 2092, 2093, 2627
AflIII	ACRYGT	1 cut(s)	2612
AjnI	CCWGG	2 cut(s)	111, 2626
AleI	CACNNNNGTG	1 cut(s)	471
Alw26I	GTCTC	8 cut(s)	220, 293, 523, 925, 1032, 1652, 1660, 2277
AlwI	GGATC	7 cut(s)	491, 626, 839, 1693, 1945, 1974, 2077
AlwNI	CAGNNNCTG	1 cut(s)	626
Aor13HI	TCCGGA	1 cut(s)	499
Aor51HI	AGCGCT	1 cut(s)	1134
AoxI	GGCC	4 cut(s)	109, 324, 376, 1105
ApeKI	GCWGC	6 cut(s)	176, 1178, 1633, 1761, 2480, 2661
ApoI	RAATTY	7 cut(s)	270, 291, 477, 733, 1386, 1707, 1850
ArsI	GACNNNNNNTTYG	8 cut(s)	132, 164, 1217, 1249, 1596, 1628, 2305, 2337
AspLEI	GCGC	4 cut(s)	1091, 1135, 1473, 2626
AspS9I	GGNCC	2 cut(s)	324, 483
AsuC2I	CCSGG	2 cut(s)	88, 108
AsuHPI	GGTGA	7 cut(s)	16, 193, 686, 880, 1606, 1739, 1931
AsuII	TTCGAA	1 cut(s)	2608
AvaII	GGWCC	1 cut(s)	483
BaeI	ACNNNNGTAYC	4 cut(s)	1500, 1533, 1560, 1593
BalI	TGGCCA	1 cut(s)	378
BanI	GGYRCC	1 cut(s)	32
BbrPI	CACGTG	1 cut(s)	910
BbvI	GCAGC	5 cut(s)	163, 1190, 1620, 1773, 2492
BceAI	ACGGC	2 cut(s)	311, 1120
BcgI	CGANNNNNNTGC	2 cut(s)	14, 48
BciT130I	CCWGG	2 cut(s)	113, 2628
BciVI	GTATCC	2 cut(s)	1501, 1561
BclI	TGATCA	1 cut(s)	1750
BcnI	CCSGG	2 cut(s)	88, 108
BcoDI	GTCTC	8 cut(s)	220, 293, 523, 925, 1032, 1652, 1660, 2277
BfaI	CTAG	6 cut(s)	1010, 1478, 1718, 1826, 2165, 2264
BfmI	CTRYAG	3 cut(s)	1092, 2088, 2659
BfoI	RGCGCY	2 cut(s)	1136, 1474
BfuI	GTATCC	2 cut(s)	1501, 1561
BglII	AGATCT	1 cut(s)	1185
BisI	GCNGC	9 cut(s)	36, 177, 194, 1179, 1634, 1762, 1960, 2481, 2662
BlpI	GCTNAGC	1 cut(s)	1136
BlsI	GCNGC	9 cut(s)	37, 178, 195, 1180, 1635, 1763, 1961, 2482, 2663
Bme1390I	CCNGG	4 cut(s)	88, 108, 113, 2628
Bme18I	GGWCC	1 cut(s)	483
BmgT120I	GGNCC	2 cut(s)	324, 483
BmiI	GGNNCC	3 cut(s)	34, 325, 1291
BmrFI	CCNGG	4 cut(s)	88, 108, 113, 2628
BmrI	ACTGGG	2 cut(s)	255, 1846
BmsI	GCATC	8 cut(s)	162, 432, 1531, 1831, 2322, 2386, 2527, 2644
BmuI	ACTGGG	2 cut(s)	255, 1846
BpmI	CTGGAG	2 cut(s)	648, 909
Bpu1102I	GCTNAGC	1 cut(s)	1136
Bpu14I	TTCGAA	1 cut(s)	2608
BpuEI	CTTGAG	4 cut(s)	1082, 1674, 2012, 2057
BpuMI	CCSGG	2 cut(s)	88, 108
Bsa29I	ATCGAT	1 cut(s)	495
BsaAI	YACGTR	1 cut(s)	910
BsaBI	GATNNNNATC	1 cut(s)	495
BsaI	GGTCTC	4 cut(s)	220, 293, 523, 925
BsaJI	CCNNGG	8 cut(s)	106, 436, 853, 864, 1102, 1213, 1293, 1915
BsaWI	WCCGGW	1 cut(s)	499
BsaXI	ACNNNNNCTCC	2 cut(s)	151, 181
Bsc4I	CCNNNNNNNGG	7 cut(s)	112, 118, 385, 1202, 2092, 2093, 2627
Bse1I	ACTGG	9 cut(s)	197, 250, 789, 961, 1167, 1637, 1687, 1841, 2373
Bse3DI	GCAATG	1 cut(s)	2490
Bse8I	GATNNNNATC	1 cut(s)	495
BseAI	TCCGGA	1 cut(s)	499
BseBI	CCWGG	2 cut(s)	113, 2628
BseCI	ATCGAT	1 cut(s)	495
BseDI	CCNNGG	8 cut(s)	106, 436, 853, 864, 1102, 1213, 1293, 1915
BseJI	GATNNNNATC	1 cut(s)	495
BseLI	CCNNNNNNNGG	7 cut(s)	112, 118, 385, 1202, 2092, 2093, 2627
BseMI	GCAATG	1 cut(s)	2490
BseMII	CTCAG	6 cut(s)	296, 1022, 1155, 1164, 1434, 2229
BseNI	ACTGG	9 cut(s)	197, 250, 789, 961, 1167, 1637, 1687, 1841, 2373
BseRI	GAGGAG	1 cut(s)	2265
BseXI	GCAGC	5 cut(s)	163, 1190, 1620, 1773, 2492
BseYI	CCCAGC	2 cut(s)	1962, 2111
BsgI	GTGCAG	1 cut(s)	2396
Bsh1236I	CGCG	4 cut(s)	212, 438, 1089, 2624
BshFI	GGCC	4 cut(s)	111, 326, 378, 1107
BshNI	GGYRCC	1 cut(s)	32
BshVI	ATCGAT	1 cut(s)	495
BsiSI	CCGG	4 cut(s)	87, 108, 500, 829
BslFI	GGGAC	2 cut(s)	290, 1890
BslI	CCNNNNNNNGG	7 cut(s)	112, 118, 385, 1202, 2092, 2093, 2627
BsmAI	GTCTC	8 cut(s)	220, 293, 523, 925, 1032, 1652, 1660, 2277
BsmFI	GGGAC	2 cut(s)	290, 1890
BsmI	GAATGC	1 cut(s)	604
BsnI	GGCC	4 cut(s)	111, 326, 378, 1107
Bso31I	GGTCTC	4 cut(s)	220, 293, 523, 925
Bsp119I	TTCGAA	1 cut(s)	2608
Bsp13I	TCCGGA	1 cut(s)	499
Bsp1720I	GCTNAGC	1 cut(s)	1136
BspANI	GGCC	4 cut(s)	111, 326, 378, 1107
BspCNI	CTCAG	6 cut(s)	295, 1023, 1154, 1163, 1433, 2230
BspDI	ATCGAT	1 cut(s)	495
BspEI	TCCGGA	1 cut(s)	499
BspFNI	CGCG	4 cut(s)	212, 438, 1089, 2624
BspHI	TCATGA	2 cut(s)	1882, 1942
BspLI	GGNNCC	3 cut(s)	34, 325, 1291
BspMAI	CTGCAG	2 cut(s)	2092, 2663
BspPI	GGATC	7 cut(s)	491, 626, 839, 1693, 1945, 1974, 2077
BspT104I	TTCGAA	1 cut(s)	2608
BspT107I	GGYRCC	1 cut(s)	32
BspTNI	GGTCTC	4 cut(s)	220, 293, 523, 925
BsrDI	GCAATG	1 cut(s)	2490
BsrI	ACTGG	9 cut(s)	197, 250, 789, 961, 1167, 1637, 1687, 1841, 2373
BssECI	CCNNGG	8 cut(s)	106, 436, 853, 864, 1102, 1213, 1293, 1915
BssT1I	CCWWGG	3 cut(s)	853, 1213, 1293
Bst2UI	CCWGG	2 cut(s)	113, 2628
Bst4CI	ACNGT	7 cut(s)	83, 314, 342, 712, 1336, 1916, 2645
Bst6I	CTCTTC	2 cut(s)	888, 1992
BstBAI	YACGTR	1 cut(s)	910
BstBI	TTCGAA	1 cut(s)	2608
BstC8I	GCNNGC	5 cut(s)	440, 577, 741, 1475, 2633
BstDEI	CTNAG	9 cut(s)	282, 542, 1031, 1136, 1141, 1150, 1420, 1467, 2238
BstDSI	CCRYGG	3 cut(s)	436, 1102, 1915
BstENI	CCTNNNNNAGG	1 cut(s)	116
BstFNI	CGCG	4 cut(s)	212, 438, 1089, 2624
BstH2I	RGCGCY	2 cut(s)	1136, 1474
BstHHI	GCGC	4 cut(s)	1091, 1135, 1473, 2626
BstMAI	GTCTC	8 cut(s)	220, 293, 523, 925, 1032, 1652, 1660, 2277
BstNI	CCWGG	2 cut(s)	113, 2628
BstNSI	RCATGY	3 cut(s)	747, 2616, 2635
BstSCI	CCNGG	4 cut(s)	86, 106, 111, 2626
BstSFI	CTRYAG	3 cut(s)	1092, 2088, 2659
BstUI	CGCG	4 cut(s)	212, 438, 1089, 2624
BstV1I	GCAGC	5 cut(s)	163, 1190, 1620, 1773, 2492
BstX2I	RGATCY	5 cut(s)	631, 1185, 1685, 1937, 2082
BstYI	RGATCY	5 cut(s)	631, 1185, 1685, 1937, 2082
Bsu15I	ATCGAT	1 cut(s)	495
BsuI	GTATCC	2 cut(s)	1501, 1561
BsuRI	GGCC	4 cut(s)	111, 326, 378, 1107
BsuTUI	ATCGAT	1 cut(s)	495
BtgI	CCRYGG	3 cut(s)	436, 1102, 1915
BtgZI	GCGATG	2 cut(s)	55, 193
BtsIMutI	CAGTG	5 cut(s)	243, 1338, 1644, 2227, 2366
Cac8I	GCNNGC	5 cut(s)	440, 577, 741, 1475, 2633
CaiI	CAGNNNCTG	1 cut(s)	626
CciI	TCATGA	2 cut(s)	1882, 1942
CfoI	GCGC	4 cut(s)	1091, 1135, 1473, 2626
Cfr13I	GGNCC	2 cut(s)	324, 483
Cfr42I	CCGCGG	1 cut(s)	439
ClaI	ATCGAT	1 cut(s)	495
Csp6I	GTAC	7 cut(s)	13, 343, 767, 782, 1775, 1971, 2029
CviQI	GTAC	7 cut(s)	13, 343, 767, 782, 1775, 1971, 2029
DdeI	CTNAG	9 cut(s)	282, 542, 1031, 1136, 1141, 1150, 1420, 1467, 2238
DraIII	CACNNNGTG	1 cut(s)	1361
EaeI	YGGCCR	2 cut(s)	376, 1105
Eam1104I	CTCTTC	2 cut(s)	888, 1992
EarI	CTCTTC	2 cut(s)	888, 1992
EciI	GGCGGA	1 cut(s)	2225
Eco130I	CCWWGG	3 cut(s)	853, 1213, 1293
Eco31I	GGTCTC	4 cut(s)	220, 293, 523, 925
Eco32I	GATATC	2 cut(s)	493, 2461
Eco47I	GGWCC	1 cut(s)	483
Eco47III	AGCGCT	1 cut(s)	1134
Eco57I	CTGAAG	1 cut(s)	1362
Eco72I	CACGTG	1 cut(s)	910
EcoNI	CCTNNNNNAGG	1 cut(s)	116
EcoRI	GAATTC	1 cut(s)	1386
EcoRII	CCWGG	2 cut(s)	111, 2626
EcoRV	GATATC	2 cut(s)	493, 2461
EcoT14I	CCWWGG	3 cut(s)	853, 1213, 1293
EcoT22I	ATGCAT	1 cut(s)	2637
ErhI	CCWWGG	3 cut(s)	853, 1213, 1293
FalI	AAGNNNNNCTT	1 cut(s)	31
FaqI	GGGAC	2 cut(s)	290, 1890
FauI	CCCGC	3 cut(s)	431, 2004, 2629
FbaI	TGATCA	1 cut(s)	1750
FblI	GTMKAC	1 cut(s)	2434
Fnu4HI	GCNGC	9 cut(s)	36, 177, 194, 1179, 1634, 1762, 1960, 2481, 2662
Fsp4HI	GCNGC	9 cut(s)	36, 177, 194, 1179, 1634, 1762, 1960, 2481, 2662
FspBI	CTAG	6 cut(s)	1010, 1478, 1718, 1826, 2165, 2264
GlaI	GCGC	4 cut(s)	1090, 1134, 1472, 2625
GluI	GCNGC	9 cut(s)	36, 177, 194, 1179, 1634, 1762, 1960, 2481, 2662
GsaI	CCCAGC	2 cut(s)	1966, 2115
GsuI	CTGGAG	2 cut(s)	648, 909
HaeII	RGCGCY	2 cut(s)	1136, 1474
HaeIII	GGCC	4 cut(s)	111, 326, 378, 1107
HapII	CCGG	4 cut(s)	87, 108, 500, 829
HhaI	GCGC	4 cut(s)	1091, 1135, 1473, 2626
Hin6I	GCGC	4 cut(s)	1089, 1133, 1471, 2624
HinP1I	GCGC	4 cut(s)	1089, 1133, 1471, 2624
HincII	GTYRAC	1 cut(s)	1164
HindII	GTYRAC	1 cut(s)	1164
HindIII	AAGCTT	1 cut(s)	573
HpaII	CCGG	4 cut(s)	87, 108, 500, 829
HphI	GGTGA	7 cut(s)	16, 193, 686, 880, 1606, 1739, 1931
Hpy166II	GTNNAC	5 cut(s)	550, 1164, 1831, 2435, 2618
Hpy8I	GTNNAC	5 cut(s)	550, 1164, 1831, 2435, 2618
Hpy99I	CGWCG	1 cut(s)	2196
HpyCH4III	ACNGT	7 cut(s)	83, 314, 342, 712, 1336, 1916, 2645
HpyCH4IV	ACGT	5 cut(s)	135, 780, 909, 1320, 2437
HpyF3I	CTNAG	9 cut(s)	282, 542, 1031, 1136, 1141, 1150, 1420, 1467, 2238
HpySE526I	ACGT	5 cut(s)	135, 780, 909, 1320, 2437
HspAI	GCGC	4 cut(s)	1089, 1133, 1471, 2624
Kpn2I	TCCGGA	1 cut(s)	499
Ksp22I	TGATCA	1 cut(s)	1750
KspI	CCGCGG	1 cut(s)	439
LmnI	GCTCC	3 cut(s)	261, 884, 2096
Lsp1109I	GCAGC	5 cut(s)	163, 1190, 1620, 1773, 2492
LweI	GCATC	8 cut(s)	162, 432, 1531, 1831, 2322, 2386, 2527, 2644
MaeI	CTAG	6 cut(s)	1010, 1478, 1718, 1826, 2165, 2264
MaeII	ACGT	5 cut(s)	135, 780, 909, 1320, 2437
MfeI	CAATTG	2 cut(s)	2151, 2501
MflI	RGATCY	5 cut(s)	631, 1185, 1685, 1937, 2082
MlsI	TGGCCA	1 cut(s)	378
MluNI	TGGCCA	1 cut(s)	378
MlyI	GAGTC	7 cut(s)	272, 617, 1461, 2042, 2141, 2336, 2482
MmeI	TCCRAC	4 cut(s)	1220, 1267, 1545, 1998
Mox20I	TGGCCA	1 cut(s)	378
Mph1103I	ATGCAT	1 cut(s)	2637
MroI	TCCGGA	1 cut(s)	499
MscI	TGGCCA	1 cut(s)	378
MseI	TTAA	7 cut(s)	164, 558, 570, 1434, 1865, 2310, 2354
MslI	CAYNNNNRTG	2 cut(s)	471, 1887
Msp20I	TGGCCA	1 cut(s)	378
MspA1I	CMGCKG	2 cut(s)	438, 1962
MspI	CCGG	4 cut(s)	87, 108, 500, 829
MspR9I	CCNGG	4 cut(s)	88, 108, 113, 2628
MunI	CAATTG	2 cut(s)	2151, 2501
Mva1269I	GAATGC	1 cut(s)	604
MvaI	CCWGG	2 cut(s)	113, 2628
MvnI	CGCG	4 cut(s)	212, 438, 1089, 2624
NciI	CCSGG	2 cut(s)	88, 108
NlaIV	GGNNCC	3 cut(s)	34, 325, 1291
NmeAIII	GCCGAG	1 cut(s)	889
NsiI	ATGCAT	1 cut(s)	2637
NspI	RCATGY	3 cut(s)	747, 2616, 2635
NspV	TTCGAA	1 cut(s)	2608
OliI	CACNNNNGTG	1 cut(s)	471
PaeI	GCATGC	1 cut(s)	2635
PagI	TCATGA	2 cut(s)	1882, 1942
PciI	ACATGT	1 cut(s)	2612
PctI	GAATGC	1 cut(s)	604
PflMI	CCANNNNNTGG	1 cut(s)	1202
PfoI	TCCNGGA	1 cut(s)	86
PkrI	GCNGC	9 cut(s)	37, 178, 195, 1180, 1635, 1763, 1961, 2482, 2663
PleI	GAGTC	7 cut(s)	272, 617, 1460, 2041, 2141, 2335, 2482
PmaCI	CACGTG	1 cut(s)	910
PmlI	CACGTG	1 cut(s)	910
PpsI	GAGTC	7 cut(s)	272, 617, 1460, 2041, 2141, 2335, 2482
Ppu21I	YACGTR	1 cut(s)	910
PscI	ACATGT	1 cut(s)	2612
Psp6I	CCWGG	2 cut(s)	111, 2626
PspCI	CACGTG	1 cut(s)	910
PspFI	CCCAGC	2 cut(s)	1962, 2111
PspGI	CCWGG	2 cut(s)	111, 2626
PspN4I	GGNNCC	3 cut(s)	34, 325, 1291
PspPI	GGNCC	2 cut(s)	324, 483
PstI	CTGCAG	2 cut(s)	2092, 2663
PstNI	CAGNNNCTG	1 cut(s)	626
PsuI	RGATCY	5 cut(s)	631, 1185, 1685, 1937, 2082
RsaI	GTAC	7 cut(s)	14, 344, 768, 783, 1776, 1972, 2030
RsaNI	GTAC	7 cut(s)	13, 343, 767, 782, 1775, 1971, 2029
RseI	CAYNNNNRTG	2 cut(s)	471, 1887
SacII	CCGCGG	1 cut(s)	439
SaqAI	TTAA	7 cut(s)	164, 558, 570, 1434, 1865, 2310, 2354
SatI	GCNGC	9 cut(s)	36, 177, 194, 1179, 1634, 1762, 1960, 2481, 2662
Sau96I	GGNCC	2 cut(s)	324, 483
SchI	GAGTC	7 cut(s)	272, 617, 1461, 2042, 2141, 2336, 2482
ScrFI	CCNGG	4 cut(s)	88, 108, 113, 2628
SfaNI	GCATC	8 cut(s)	162, 432, 1531, 1831, 2322, 2386, 2527, 2644
SfcI	CTRYAG	3 cut(s)	1092, 2088, 2659
Sfr303I	CCGCGG	1 cut(s)	439
SfuI	TTCGAA	1 cut(s)	2608
SgrBI	CCGCGG	1 cut(s)	439
SinI	GGWCC	1 cut(s)	483
SmiMI	CAYNNNNRTG	2 cut(s)	471, 1887
SmlI	CTYRAG	4 cut(s)	1061, 1689, 1991, 2072
SmoI	CTYRAG	4 cut(s)	1061, 1689, 1991, 2072
SphI	GCATGC	1 cut(s)	2635
SspMI	CTAG	6 cut(s)	1010, 1478, 1718, 1826, 2165, 2264
StyD4I	CCNGG	4 cut(s)	86, 106, 111, 2626
StyI	CCWWGG	3 cut(s)	853, 1213, 1293
TaaI	ACNGT	7 cut(s)	83, 314, 342, 712, 1336, 1916, 2645
TaiI	ACGT	5 cut(s)	138, 783, 912, 1323, 2440
TaqI	TCGA	5 cut(s)	495, 1650, 2330, 2608, 2638
TaqII	GACCGA	1 cut(s)	2159
TatI	WGTACW	1 cut(s)	1774
TauI	GCSGC	3 cut(s)	38, 196, 1962
Tru1I	TTAA	7 cut(s)	164, 558, 570, 1434, 1865, 2310, 2354
Tru9I	TTAA	7 cut(s)	164, 558, 570, 1434, 1865, 2310, 2354
TscAI	CASTG	5 cut(s)	250, 1345, 1644, 2227, 2373
TseI	GCWGC	6 cut(s)	176, 1178, 1633, 1761, 2480, 2661
TspDTI	ATGAA	6 cut(s)	887, 1410, 1511, 1886, 2064, 2310
TspRI	CASTG	5 cut(s)	250, 1345, 1644, 2227, 2373
Van91I	CCANNNNNTGG	1 cut(s)	1202
VpaK11BI	GGWCC	1 cut(s)	483
XagI	CCTNNNNNAGG	1 cut(s)	116
XapI	RAATTY	7 cut(s)	270, 291, 477, 733, 1386, 1707, 1850
XceI	RCATGY	3 cut(s)	747, 2616, 2635
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1552
XmiI	GTMKAC	1 cut(s)	2434
XspI	CTAG	6 cut(s)	1010, 1478, 1718, 1826, 2165, 2264
Zsp2I	ATGCAT	1 cut(s)	2637

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.