Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 177 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 410 |
| Acc36I | ACCTGC | 1 cut(s) | 921 |
| AccB1I | GGYRCC | 1 cut(s) | 1326 |
| AccI | GTMKAC | 1 cut(s) | 801 |
| AccII | CGCG | 1 cut(s) | 66 |
| AciI | CCGC | 9 cut(s) | 420, 538, 570, 585, 928, 1119, 1277, 1346, 1382 |
| AcoI | YGGCCR | 4 cut(s) | 388, 1278, 1284, 1391 |
| AcsI | RAATTY | 6 cut(s) | 16, 81, 138, 179, 205, 219 |
| AcuI | CTGAAG | 1 cut(s) | 430 |
| AcyI | GRCGYC | 2 cut(s) | 582, 1184 |
| AfaI | GTAC | 2 cut(s) | 676, 1244 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 125, 1214, 1395 |
| AhlI | ACTAGT | 1 cut(s) | 23 |
| AjiI | CACGTC | 2 cut(s) | 472, 1042 |
| AjnI | CCWGG | 3 cut(s) | 599, 1377, 1388 |
| AloI | GAACNNNNNNTCC | 4 cut(s) | 360, 392, 1242, 1274 |
| AluBI | AGCT | 6 cut(s) | 108, 355, 544, 562, 661, 1144 |
| AluI | AGCT | 6 cut(s) | 108, 355, 544, 562, 661, 1144 |
| Alw21I | GWGCWC | 3 cut(s) | 357, 879, 1030 |
| Alw26I | GTCTC | 5 cut(s) | 417, 813, 823, 892, 939 |
| Alw44I | GTGCAC | 1 cut(s) | 1026 |
| Ama87I | CYCGRG | 1 cut(s) | 497 |
| AoxI | GGCC | 9 cut(s) | 321, 388, 604, 730, 1128, 1227, 1278, 1284, 1391 |
| ApaLI | GTGCAC | 1 cut(s) | 1026 |
| ApeKI | GCWGC | 2 cut(s) | 559, 1141 |
| ApoI | RAATTY | 6 cut(s) | 16, 81, 138, 179, 205, 219 |
| AspLEI | GCGC | 2 cut(s) | 68, 1172 |
| AspS9I | GGNCC | 6 cut(s) | 112, 493, 525, 604, 1066, 1305 |
| AsuC2I | CCSGG | 1 cut(s) | 554 |
| AsuHPI | GGTGA | 1 cut(s) | 532 |
| AvaI | CYCGRG | 1 cut(s) | 497 |
| AvaII | GGWCC | 5 cut(s) | 112, 493, 525, 1066, 1305 |
| AxyI | CCTNAGG | 1 cut(s) | 813 |
| BaeGI | GKGCMC | 1 cut(s) | 1030 |
| BalI | TGGCCA | 3 cut(s) | 390, 1286, 1393 |
| BanI | GGYRCC | 1 cut(s) | 1326 |
| BanII | GRGCYC | 2 cut(s) | 357, 504 |
| BauI | CACGAG | 1 cut(s) | 1136 |
| BbsI | GAAGAC | 1 cut(s) | 585 |
| Bbv12I | GWGCWC | 3 cut(s) | 357, 879, 1030 |
| BbvI | GCAGC | 2 cut(s) | 571, 1153 |
| BccI | CCATC | 4 cut(s) | 314, 380, 920, 1186 |
| BceAI | ACGGC | 4 cut(s) | 388, 467, 692, 1265 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 919, 953 |
| BciT130I | CCWGG | 3 cut(s) | 601, 1379, 1390 |
| BcnI | CCSGG | 1 cut(s) | 554 |
| BcoDI | GTCTC | 5 cut(s) | 417, 813, 823, 892, 939 |
| BcuI | ACTAGT | 1 cut(s) | 23 |
| BfaI | CTAG | 2 cut(s) | 9, 24 |
| BfoI | RGCGCY | 1 cut(s) | 1173 |
| BfuAI | ACCTGC | 1 cut(s) | 921 |
| BisI | GCNGC | 8 cut(s) | 560, 571, 585, 928, 1142, 1278, 1347, 1383 |
| BlsI | GCNGC | 8 cut(s) | 561, 572, 586, 929, 1143, 1279, 1348, 1384 |
| Bme1390I | CCNGG | 4 cut(s) | 554, 601, 1379, 1390 |
| Bme18I | GGWCC | 5 cut(s) | 112, 493, 525, 1066, 1305 |
| BmeT110I | CYCGRG | 1 cut(s) | 497 |
| BmgBI | CACGTC | 2 cut(s) | 472, 1042 |
| BmgT120I | GGNCC | 6 cut(s) | 112, 493, 525, 604, 1066, 1305 |
| BmiI | GGNNCC | 8 cut(s) | 113, 114, 460, 605, 721, 1068, 1260, 1328 |
| BmrFI | CCNGG | 4 cut(s) | 554, 601, 1379, 1390 |
| BmrI | ACTGGG | 1 cut(s) | 1343 |
| BmsI | GCATC | 3 cut(s) | 309, 384, 1358 |
| BmuI | ACTGGG | 1 cut(s) | 1343 |
| BpiI | GAAGAC | 1 cut(s) | 585 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 287, 319, 1130, 1162 |
| BpuEI | CTTGAG | 1 cut(s) | 52 |
| BpuMI | CCSGG | 1 cut(s) | 554 |
| Bsa29I | ATCGAT | 1 cut(s) | 513 |
| BsaHI | GRCGYC | 2 cut(s) | 582, 1184 |
| BsaI | GGTCTC | 2 cut(s) | 823, 939 |
| BsaJI | CCNNGG | 6 cut(s) | 124, 600, 733, 1208, 1281, 1394 |
| BsaWI | WCCGGW | 1 cut(s) | 455 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1242, 1272 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 125, 1214, 1395 |
| Bse118I | RCCGGY | 3 cut(s) | 527, 722, 1180 |
| Bse1I | ACTGG | 2 cut(s) | 1328, 1338 |
| Bse21I | CCTNAGG | 1 cut(s) | 813 |
| BseBI | CCWGG | 3 cut(s) | 601, 1379, 1390 |
| BseCI | ATCGAT | 1 cut(s) | 513 |
| BseDI | CCNNGG | 6 cut(s) | 124, 600, 733, 1208, 1281, 1394 |
| BseGI | GGATG | 3 cut(s) | 192, 306, 375 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 125, 1214, 1395 |
| BseMII | CTCAG | 2 cut(s) | 827, 998 |
| BseNI | ACTGG | 2 cut(s) | 1328, 1338 |
| BseRI | GAGGAG | 2 cut(s) | 801, 887 |
| BseSI | GKGCMC | 1 cut(s) | 1030 |
| BseX3I | CGGCCG | 1 cut(s) | 1278 |
| BseXI | GCAGC | 2 cut(s) | 571, 1153 |
| BseYI | CCCAGC | 2 cut(s) | 108, 348 |
| BsgI | GTGCAG | 1 cut(s) | 221 |
| Bsh1236I | CGCG | 1 cut(s) | 66 |
| Bsh1285I | CGRYCG | 2 cut(s) | 57, 1281 |
| BshFI | GGCC | 9 cut(s) | 323, 390, 606, 732, 1130, 1229, 1280, 1286, 1393 |
| BshNI | GGYRCC | 1 cut(s) | 1326 |
| BshVI | ATCGAT | 1 cut(s) | 513 |
| BsiEI | CGRYCG | 2 cut(s) | 57, 1281 |
| BsiHKAI | GWGCWC | 3 cut(s) | 357, 879, 1030 |
| BsiHKCI | CYCGRG | 1 cut(s) | 497 |
| BsiSI | CCGG | 7 cut(s) | 324, 456, 462, 528, 554, 723, 1181 |
| BslFI | GGGAC | 3 cut(s) | 125, 458, 1052 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 125, 1214, 1395 |
| BsmAI | GTCTC | 5 cut(s) | 417, 813, 823, 892, 939 |
| BsmBI | CGTCTC | 2 cut(s) | 417, 813 |
| BsmFI | GGGAC | 3 cut(s) | 125, 458, 1052 |
| BsnI | GGCC | 9 cut(s) | 323, 390, 606, 732, 1130, 1229, 1280, 1286, 1393 |
| Bso31I | GGTCTC | 2 cut(s) | 823, 939 |
| BsoBI | CYCGRG | 1 cut(s) | 497 |
| Bsp1286I | GDGCHC | 4 cut(s) | 357, 504, 879, 1030 |
| Bsp1407I | TGTACA | 1 cut(s) | 1242 |
| Bsp143I | GATC | 7 cut(s) | 54, 634, 646, 978, 1113, 1158, 1290 |
| BspACI | CCGC | 9 cut(s) | 420, 538, 570, 585, 928, 1119, 1277, 1346, 1382 |
| BspANI | GGCC | 9 cut(s) | 323, 390, 606, 732, 1130, 1229, 1280, 1286, 1393 |
| BspCNI | CTCAG | 2 cut(s) | 826, 997 |
| BspDI | ATCGAT | 1 cut(s) | 513 |
| BspFNI | CGCG | 1 cut(s) | 66 |
| BspLI | GGNNCC | 8 cut(s) | 113, 114, 460, 605, 721, 1068, 1260, 1328 |
| BspMI | ACCTGC | 1 cut(s) | 921 |
| BspT107I | GGYRCC | 1 cut(s) | 1326 |
| BspTNI | GGTCTC | 2 cut(s) | 823, 939 |
| BsrFI | RCCGGY | 3 cut(s) | 527, 722, 1180 |
| BsrGI | TGTACA | 1 cut(s) | 1242 |
| BsrI | ACTGG | 2 cut(s) | 1328, 1338 |
| BssAI | RCCGGY | 3 cut(s) | 527, 722, 1180 |
| BssECI | CCNNGG | 6 cut(s) | 124, 600, 733, 1208, 1281, 1394 |
| BssMI | GATC | 7 cut(s) | 54, 634, 646, 978, 1113, 1158, 1290 |
| BssNI | GRCGYC | 2 cut(s) | 582, 1184 |
| BssSI | CACGAG | 1 cut(s) | 1136 |
| BssT1I | CCWWGG | 2 cut(s) | 1208, 1394 |
| Bst2BI | CACGAG | 1 cut(s) | 1136 |
| Bst2UI | CCWGG | 3 cut(s) | 601, 1379, 1390 |
| Bst4CI | ACNGT | 8 cut(s) | 97, 173, 412, 508, 674, 799, 1025, 1371 |
| Bst6I | CTCTTC | 1 cut(s) | 258 |
| BstACI | GRCGYC | 2 cut(s) | 582, 1184 |
| BstAUI | TGTACA | 1 cut(s) | 1242 |
| BstC8I | GCNNGC | 6 cut(s) | 346, 542, 663, 851, 1182, 1231 |
| BstDEI | CTNAG | 2 cut(s) | 813, 984 |
| BstDSI | CCRYGG | 1 cut(s) | 1281 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1393 |
| BstF5I | GGATG | 3 cut(s) | 192, 306, 375 |
| BstFNI | CGCG | 1 cut(s) | 66 |
| BstH2I | RGCGCY | 1 cut(s) | 1173 |
| BstHHI | GCGC | 2 cut(s) | 68, 1172 |
| BstKTI | GATC | 7 cut(s) | 57, 637, 649, 981, 1116, 1161, 1293 |
| BstMAI | GTCTC | 5 cut(s) | 417, 813, 823, 892, 939 |
| BstMBI | GATC | 7 cut(s) | 54, 634, 646, 978, 1113, 1158, 1290 |
| BstMCI | CGRYCG | 2 cut(s) | 57, 1281 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 244, 537, 1127, 1283 |
| BstNI | CCWGG | 3 cut(s) | 601, 1379, 1390 |
| BstSCI | CCNGG | 4 cut(s) | 552, 599, 1377, 1388 |
| BstSLI | GKGCMC | 1 cut(s) | 1030 |
| BstUI | CGCG | 1 cut(s) | 66 |
| BstV1I | GCAGC | 2 cut(s) | 571, 1153 |
| BstV2I | GAAGAC | 1 cut(s) | 585 |
| BstXI | CCANNNNNNTGG | 3 cut(s) | 159, 386, 710 |
| BstZI | CGGCCG | 1 cut(s) | 1278 |
| Bsu15I | ATCGAT | 1 cut(s) | 513 |
| Bsu36I | CCTNAGG | 1 cut(s) | 813 |
| BsuRI | GGCC | 9 cut(s) | 323, 390, 606, 732, 1130, 1229, 1280, 1286, 1393 |
| BsuTUI | ATCGAT | 1 cut(s) | 513 |
| BtgI | CCRYGG | 1 cut(s) | 1281 |
| BtrI | CACGTC | 2 cut(s) | 472, 1042 |
| BtsCI | GGATG | 3 cut(s) | 192, 306, 375 |
| BtsI | GCAGTG | 1 cut(s) | 1002 |
| BtsIMutI | CAGTG | 1 cut(s) | 1002 |
| BveI | ACCTGC | 1 cut(s) | 921 |
| Cac8I | GCNNGC | 6 cut(s) | 346, 542, 663, 851, 1182, 1231 |
| CfoI | GCGC | 2 cut(s) | 68, 1172 |
| Cfr10I | RCCGGY | 3 cut(s) | 527, 722, 1180 |
| Cfr13I | GGNCC | 6 cut(s) | 112, 493, 525, 604, 1066, 1305 |
| ClaI | ATCGAT | 1 cut(s) | 513 |
| CseI | GACGC | 3 cut(s) | 437, 590, 1173 |
| Csp6I | GTAC | 2 cut(s) | 675, 1243 |
| CviAII | CATG | 4 cut(s) | 144, 1090, 1225, 1416 |
| CviQI | GTAC | 2 cut(s) | 675, 1243 |
| DdeI | CTNAG | 2 cut(s) | 813, 984 |
| DpnI | GATC | 7 cut(s) | 56, 636, 648, 980, 1115, 1160, 1292 |
| DpnII | GATC | 7 cut(s) | 54, 634, 646, 978, 1113, 1158, 1290 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 410 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 410 |
| EaeI | YGGCCR | 4 cut(s) | 388, 1278, 1284, 1391 |
| EagI | CGGCCG | 1 cut(s) | 1278 |
| Eam1104I | CTCTTC | 1 cut(s) | 258 |
| EarI | CTCTTC | 1 cut(s) | 258 |
| EciI | GGCGGA | 1 cut(s) | 435 |
| Ecl136II | GAGCTC | 1 cut(s) | 355 |
| EclXI | CGGCCG | 1 cut(s) | 1278 |
| Eco130I | CCWWGG | 2 cut(s) | 1208, 1394 |
| Eco147I | AGGCCT | 1 cut(s) | 1130 |
| Eco24I | GRGCYC | 2 cut(s) | 357, 504 |
| Eco31I | GGTCTC | 2 cut(s) | 823, 939 |
| Eco47I | GGWCC | 5 cut(s) | 112, 493, 525, 1066, 1305 |
| Eco52I | CGGCCG | 1 cut(s) | 1278 |
| Eco53kI | GAGCTC | 1 cut(s) | 355 |
| Eco57I | CTGAAG | 1 cut(s) | 430 |
| Eco81I | CCTNAGG | 1 cut(s) | 813 |
| Eco88I | CYCGRG | 1 cut(s) | 497 |
| EcoICRI | GAGCTC | 1 cut(s) | 355 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1393 |
| EcoO109I | RGGNCCY | 1 cut(s) | 112 |
| EcoRI | GAATTC | 1 cut(s) | 16 |
| EcoRII | CCWGG | 3 cut(s) | 599, 1377, 1388 |
| EcoT14I | CCWWGG | 2 cut(s) | 1208, 1394 |
| EcoT38I | GRGCYC | 2 cut(s) | 357, 504 |
| ErhI | CCWWGG | 2 cut(s) | 1208, 1394 |
| Esp3I | CGTCTC | 2 cut(s) | 417, 813 |
| FaeI | CATG | 4 cut(s) | 147, 1093, 1228, 1419 |
| FaiI | YATR | 9 cut(s) | 103, 145, 177, 841, 1091, 1102, 1190, 1226, 1417 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 940, 972 |
| FaqI | GGGAC | 3 cut(s) | 125, 458, 1052 |
| FatI | CATG | 4 cut(s) | 143, 1089, 1224, 1415 |
| FblI | GTMKAC | 1 cut(s) | 801 |
| Fnu4HI | GCNGC | 8 cut(s) | 560, 571, 585, 928, 1142, 1278, 1347, 1383 |
| FokI | GGATG | 3 cut(s) | 179, 293, 362 |
| FriOI | GRGCYC | 2 cut(s) | 357, 504 |
| Fsp4HI | GCNGC | 8 cut(s) | 560, 571, 585, 928, 1142, 1278, 1347, 1383 |
| FspBI | CTAG | 2 cut(s) | 9, 24 |
| GlaI | GCGC | 2 cut(s) | 67, 1171 |
| GluI | GCNGC | 8 cut(s) | 560, 571, 585, 928, 1142, 1278, 1347, 1383 |
| GsaI | CCCAGC | 2 cut(s) | 112, 352 |
| HaeII | RGCGCY | 1 cut(s) | 1173 |
| HaeIII | GGCC | 9 cut(s) | 323, 390, 606, 732, 1130, 1229, 1280, 1286, 1393 |
| HapII | CCGG | 7 cut(s) | 324, 456, 462, 528, 554, 723, 1181 |
| HgaI | GACGC | 3 cut(s) | 437, 590, 1173 |
| HhaI | GCGC | 2 cut(s) | 68, 1172 |
| Hin1I | GRCGYC | 2 cut(s) | 582, 1184 |
| Hin1II | CATG | 4 cut(s) | 147, 1093, 1228, 1419 |
| Hin6I | GCGC | 2 cut(s) | 66, 1170 |
| HinP1I | GCGC | 2 cut(s) | 66, 1170 |
| HindIII | AAGCTT | 1 cut(s) | 659 |
| HinfI | GANTC | 5 cut(s) | 4, 50, 327, 651, 1080 |
| HpaII | CCGG | 7 cut(s) | 324, 456, 462, 528, 554, 723, 1181 |
| HphI | GGTGA | 1 cut(s) | 532 |
| Hpy166II | GTNNAC | 5 cut(s) | 795, 802, 1028, 1039, 1321 |
| Hpy188I | TCNGA | 4 cut(s) | 338, 656, 1263, 1403 |
| Hpy188III | TCNNGA | 4 cut(s) | 259, 938, 1054, 1294 |
| Hpy8I | GTNNAC | 5 cut(s) | 795, 802, 1028, 1039, 1321 |
| Hpy99I | CGWCG | 3 cut(s) | 416, 431, 833 |
| HpyAV | CCTTC | 3 cut(s) | 206, 451, 616 |
| HpyCH4III | ACNGT | 8 cut(s) | 97, 173, 412, 508, 674, 799, 1025, 1371 |
| HpyCH4IV | ACGT | 2 cut(s) | 471, 1041 |
| HpyCH4V | TGCA | 5 cut(s) | 238, 300, 1028, 1154, 1415 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 244, 537, 1127, 1283 |
| HpyF3I | CTNAG | 2 cut(s) | 813, 984 |
| HpySE526I | ACGT | 2 cut(s) | 471, 1041 |
| Hsp92I | GRCGYC | 2 cut(s) | 582, 1184 |
| Hsp92II | CATG | 4 cut(s) | 147, 1093, 1228, 1419 |
| HspAI | GCGC | 2 cut(s) | 66, 1170 |
| KflI | GGGWCCC | 1 cut(s) | 112 |
| KroI | GCCGGC | 1 cut(s) | 1180 |
| KroNI | GCCGGC | 1 cut(s) | 1182 |
| Kzo9I | GATC | 7 cut(s) | 54, 634, 646, 978, 1113, 1158, 1290 |
| LmnI | GCTCC | 4 cut(s) | 352, 458, 556, 874 |
| Lsp1109I | GCAGC | 2 cut(s) | 571, 1153 |
| LweI | GCATC | 3 cut(s) | 309, 384, 1358 |
| MaeI | CTAG | 2 cut(s) | 9, 24 |
| MaeII | ACGT | 2 cut(s) | 471, 1041 |
| MaeIII | GTNAC | 3 cut(s) | 97, 401, 1042 |
| MalI | GATC | 7 cut(s) | 56, 636, 648, 980, 1115, 1160, 1292 |
| MboI | GATC | 7 cut(s) | 54, 634, 646, 978, 1113, 1158, 1290 |
| MboII | GAAGA | 3 cut(s) | 245, 436, 590 |
| MfeI | CAATTG | 1 cut(s) | 615 |
| MhlI | GDGCHC | 4 cut(s) | 357, 504, 879, 1030 |
| MlsI | TGGCCA | 3 cut(s) | 390, 1286, 1393 |
| MluNI | TGGCCA | 3 cut(s) | 390, 1286, 1393 |
| MlyI | GAGTC | 1 cut(s) | 59 |
| MmeI | TCCRAC | 2 cut(s) | 316, 691 |
| Mox20I | TGGCCA | 3 cut(s) | 390, 1286, 1393 |
| MroNI | GCCGGC | 1 cut(s) | 1180 |
| MscI | TGGCCA | 3 cut(s) | 390, 1286, 1393 |
| MslI | CAYNNNNRTG | 1 cut(s) | 882 |
| Msp20I | TGGCCA | 3 cut(s) | 390, 1286, 1393 |
| MspA1I | CMGCKG | 3 cut(s) | 108, 544, 570 |
| MspI | CCGG | 7 cut(s) | 324, 456, 462, 528, 554, 723, 1181 |
| MspR9I | CCNGG | 4 cut(s) | 554, 601, 1379, 1390 |
| MunI | CAATTG | 1 cut(s) | 615 |
| MvaI | CCWGG | 3 cut(s) | 601, 1379, 1390 |
| MvnI | CGCG | 1 cut(s) | 66 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 244, 537, 1127, 1283 |
| NaeI | GCCGGC | 1 cut(s) | 1182 |
| NciI | CCSGG | 1 cut(s) | 554 |
| NdeII | GATC | 7 cut(s) | 54, 634, 646, 978, 1113, 1158, 1290 |
| NgoMIV | GCCGGC | 1 cut(s) | 1180 |
| NlaIII | CATG | 4 cut(s) | 147, 1093, 1228, 1419 |
| NlaIV | GGNNCC | 8 cut(s) | 113, 114, 460, 605, 721, 1068, 1260, 1328 |
| NmeAIII | GCCGAG | 3 cut(s) | 105, 423, 714 |
| NmuCI | GTSAC | 3 cut(s) | 97, 401, 1042 |
| PaeR7I | CTCGAG | 1 cut(s) | 497 |
| PceI | AGGCCT | 1 cut(s) | 1130 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 504 |
| PdiI | GCCGGC | 1 cut(s) | 1182 |
| PfeI | GAWTC | 3 cut(s) | 327, 651, 1080 |
| PkrI | GCNGC | 8 cut(s) | 561, 572, 586, 929, 1143, 1279, 1348, 1384 |
| Ple19I | CGATCG | 1 cut(s) | 57 |
| PleI | GAGTC | 1 cut(s) | 58 |
| PpsI | GAGTC | 1 cut(s) | 58 |
| PpuMI | RGGWCCY | 1 cut(s) | 112 |
| PsiI | TTATAA | 1 cut(s) | 177 |
| Psp124BI | GAGCTC | 1 cut(s) | 357 |
| Psp5II | RGGWCCY | 1 cut(s) | 112 |
| Psp6I | CCWGG | 3 cut(s) | 599, 1377, 1388 |
| PspFI | CCCAGC | 2 cut(s) | 108, 348 |
| PspGI | CCWGG | 3 cut(s) | 599, 1377, 1388 |
| PspN4I | GGNNCC | 8 cut(s) | 113, 114, 460, 605, 721, 1068, 1260, 1328 |
| PspPI | GGNCC | 6 cut(s) | 112, 493, 525, 604, 1066, 1305 |
| PspPPI | RGGWCCY | 1 cut(s) | 112 |
| PspXI | VCTCGAGB | 1 cut(s) | 497 |
| PvuI | CGATCG | 1 cut(s) | 57 |
| PvuII | CAGCTG | 2 cut(s) | 108, 544 |
| RsaI | GTAC | 2 cut(s) | 676, 1244 |
| RsaNI | GTAC | 2 cut(s) | 675, 1243 |
| RseI | CAYNNNNRTG | 1 cut(s) | 882 |
| SacI | GAGCTC | 1 cut(s) | 357 |
| SatI | GCNGC | 8 cut(s) | 560, 571, 585, 928, 1142, 1278, 1347, 1383 |
| Sau3AI | GATC | 7 cut(s) | 54, 634, 646, 978, 1113, 1158, 1290 |
| Sau96I | GGNCC | 6 cut(s) | 112, 493, 525, 604, 1066, 1305 |
| SchI | GAGTC | 1 cut(s) | 59 |
| ScrFI | CCNGG | 4 cut(s) | 554, 601, 1379, 1390 |
| SduI | GDGCHC | 4 cut(s) | 357, 504, 879, 1030 |
| SfaNI | GCATC | 3 cut(s) | 309, 384, 1358 |
| Sfr274I | CTCGAG | 1 cut(s) | 497 |
| SinI | GGWCC | 5 cut(s) | 112, 493, 525, 1066, 1305 |
| SlaI | CTCGAG | 1 cut(s) | 497 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 882 |
| SmlI | CTYRAG | 2 cut(s) | 31, 497 |
| SmoI | CTYRAG | 2 cut(s) | 31, 497 |
| SpeI | ACTAGT | 1 cut(s) | 23 |
| SseBI | AGGCCT | 1 cut(s) | 1130 |
| SsiI | CCGC | 9 cut(s) | 420, 538, 570, 585, 928, 1119, 1277, 1346, 1382 |
| SspMI | CTAG | 2 cut(s) | 9, 24 |
| SstI | GAGCTC | 1 cut(s) | 357 |
| StuI | AGGCCT | 1 cut(s) | 1130 |
| StyD4I | CCNGG | 4 cut(s) | 552, 599, 1377, 1388 |
| StyI | CCWWGG | 2 cut(s) | 1208, 1394 |
| TaaI | ACNGT | 8 cut(s) | 97, 173, 412, 508, 674, 799, 1025, 1371 |
| TaiI | ACGT | 2 cut(s) | 474, 1044 |
| TaqI | TCGA | 9 cut(s) | 53, 366, 498, 513, 649, 765, 782, 900, 939 |
| TaqII | GACCGA | 1 cut(s) | 305 |
| TatI | WGTACW | 1 cut(s) | 1242 |
| TauI | GCSGC | 6 cut(s) | 573, 587, 930, 1280, 1349, 1385 |
| TfiI | GAWTC | 3 cut(s) | 327, 651, 1080 |
| TscAI | CASTG | 1 cut(s) | 1009 |
| TseFI | GTSAC | 3 cut(s) | 97, 401, 1042 |
| TseI | GCWGC | 2 cut(s) | 559, 1141 |
| Tsp45I | GTSAC | 3 cut(s) | 97, 401, 1042 |
| TspDTI | ATGAA | 2 cut(s) | 132, 1078 |
| TspGWI | ACGGA | 4 cut(s) | 786, 817, 1061, 1230 |
| TspRI | CASTG | 1 cut(s) | 1009 |
| VneI | GTGCAC | 1 cut(s) | 1026 |
| VpaK11BI | GGWCC | 5 cut(s) | 112, 493, 525, 1066, 1305 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1393 |
| XapI | RAATTY | 6 cut(s) | 16, 81, 138, 179, 205, 219 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 614, 1315 |
| XhoI | CTCGAG | 1 cut(s) | 497 |
| XmiI | GTMKAC | 1 cut(s) | 801 |
| XspI | CTAG | 2 cut(s) | 9, 24 |