No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 681 |
| Acc65I | GGTACC | 1 cut(s) | 849 |
| AccB1I | GGYRCC | 1 cut(s) | 849 |
| AccBSI | CCGCTC | 1 cut(s) | 661 |
| AccI | GTMKAC | 1 cut(s) | 604 |
| AciI | CCGC | 1 cut(s) | 661 |
| AclWI | GGATC | 6 cut(s) | 408, 421, 522, 535, 547, 560 |
| AcsI | RAATTY | 1 cut(s) | 740 |
| AcuI | CTGAAG | 1 cut(s) | 92 |
| AfaI | GTAC | 4 cut(s) | 584, 793, 841, 851 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 410, 786, 801 |
| AflIII | ACRYGT | 3 cut(s) | 118, 369, 576 |
| AgsI | TTSAA | 3 cut(s) | 250, 311, 325 |
| AjiI | CACGTC | 1 cut(s) | 372 |
| AjnI | CCWGG | 2 cut(s) | 403, 716 |
| AleI | CACNNNNGTG | 1 cut(s) | 699 |
| AluBI | AGCT | 4 cut(s) | 321, 427, 542, 770 |
| AluI | AGCT | 4 cut(s) | 321, 427, 542, 770 |
| Alw26I | GTCTC | 3 cut(s) | 217, 632, 661 |
| AlwI | GGATC | 6 cut(s) | 408, 421, 522, 535, 547, 560 |
| AoxI | GGCC | 2 cut(s) | 461, 504 |
| ApoI | RAATTY | 1 cut(s) | 740 |
| Asp718I | GGTACC | 1 cut(s) | 849 |
| AspS9I | GGNCC | 2 cut(s) | 461, 505 |
| AsuHPI | GGTGA | 2 cut(s) | 736, 737 |
| BamHI | GGATCC | 3 cut(s) | 413, 527, 552 |
| BanI | GGYRCC | 1 cut(s) | 849 |
| BbvCI | CCTCAGC | 1 cut(s) | 317 |
| BceAI | ACGGC | 1 cut(s) | 448 |
| BciT130I | CCWGG | 2 cut(s) | 405, 718 |
| BcoDI | GTCTC | 3 cut(s) | 217, 632, 661 |
| Bme1390I | CCNGG | 2 cut(s) | 405, 718 |
| BmgBI | CACGTC | 1 cut(s) | 372 |
| BmgT120I | GGNCC | 2 cut(s) | 461, 505 |
| BmiI | GGNNCC | 6 cut(s) | 51, 415, 529, 554, 851, 861 |
| BmrFI | CCNGG | 2 cut(s) | 405, 718 |
| BpmI | CTGGAG | 1 cut(s) | 36 |
| Bpu10I | CCTNAGC | 3 cut(s) | 317, 423, 543 |
| BsaBI | GATNNNNATC | 4 cut(s) | 62, 412, 495, 557 |
| BsaJI | CCNNGG | 1 cut(s) | 717 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 410, 786, 801 |
| Bse1I | ACTGG | 1 cut(s) | 53 |
| Bse8I | GATNNNNATC | 4 cut(s) | 62, 412, 495, 557 |
| BseBI | CCWGG | 2 cut(s) | 405, 718 |
| BseDI | CCNNGG | 1 cut(s) | 717 |
| BseJI | GATNNNNATC | 4 cut(s) | 62, 412, 495, 557 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 410, 786, 801 |
| BseMII | CTCAG | 4 cut(s) | 331, 414, 557, 602 |
| BseNI | ACTGG | 1 cut(s) | 53 |
| BshFI | GGCC | 2 cut(s) | 463, 506 |
| BshNI | GGYRCC | 1 cut(s) | 849 |
| BslFI | GGGAC | 7 cut(s) | 147, 627, 632, 647, 813, 817, 818 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 410, 786, 801 |
| BsmAI | GTCTC | 3 cut(s) | 217, 632, 661 |
| BsmFI | GGGAC | 7 cut(s) | 147, 627, 632, 647, 813, 817, 818 |
| BsmI | GAATGC | 1 cut(s) | 815 |
| BsnI | GGCC | 2 cut(s) | 463, 506 |
| Bsp143I | GATC | 3 cut(s) | 413, 527, 552 |
| BspACI | CCGC | 1 cut(s) | 661 |
| BspANI | GGCC | 2 cut(s) | 463, 506 |
| BspCNI | CTCAG | 4 cut(s) | 330, 415, 556, 601 |
| BspHI | TCATGA | 1 cut(s) | 444 |
| BspLI | GGNNCC | 6 cut(s) | 51, 415, 529, 554, 851, 861 |
| BspPI | GGATC | 6 cut(s) | 408, 421, 522, 535, 547, 560 |
| BspT107I | GGYRCC | 1 cut(s) | 849 |
| BsrBI | CCGCTC | 1 cut(s) | 661 |
| BsrI | ACTGG | 1 cut(s) | 53 |
| BssECI | CCNNGG | 1 cut(s) | 717 |
| BssMI | GATC | 3 cut(s) | 413, 527, 552 |
| BssNAI | GTATAC | 1 cut(s) | 605 |
| Bst1107I | GTATAC | 1 cut(s) | 605 |
| Bst2UI | CCWGG | 2 cut(s) | 405, 718 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 350 |
| BstDEI | CTNAG | 4 cut(s) | 317, 423, 543, 588 |
| BstKTI | GATC | 3 cut(s) | 416, 530, 555 |
| BstMAI | GTCTC | 3 cut(s) | 217, 632, 661 |
| BstMBI | GATC | 3 cut(s) | 413, 527, 552 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 350 |
| BstNI | CCWGG | 2 cut(s) | 405, 718 |
| BstNSI | RCATGY | 1 cut(s) | 580 |
| BstSCI | CCNGG | 2 cut(s) | 403, 716 |
| BstX2I | RGATCY | 3 cut(s) | 413, 527, 552 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 701 |
| BstYI | RGATCY | 3 cut(s) | 413, 527, 552 |
| BstZ17I | GTATAC | 1 cut(s) | 605 |
| BsuRI | GGCC | 2 cut(s) | 463, 506 |
| BtrI | CACGTC | 1 cut(s) | 372 |
| BtsIMutI | CAGTG | 1 cut(s) | 60 |
| CciI | TCATGA | 1 cut(s) | 444 |
| Cfr13I | GGNCC | 2 cut(s) | 461, 505 |
| Csp6I | GTAC | 4 cut(s) | 583, 792, 840, 850 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 439, 474 |
| CviAII | CATG | 4 cut(s) | 445, 577, 856, 884 |
| CviQI | GTAC | 4 cut(s) | 583, 792, 840, 850 |
| DdeI | CTNAG | 4 cut(s) | 317, 423, 543, 588 |
| DpnI | GATC | 3 cut(s) | 415, 529, 554 |
| DpnII | GATC | 3 cut(s) | 413, 527, 552 |
| Eco57I | CTGAAG | 1 cut(s) | 92 |
| EcoRII | CCWGG | 2 cut(s) | 403, 716 |
| FaeI | CATG | 4 cut(s) | 448, 580, 859, 887 |
| FaqI | GGGAC | 7 cut(s) | 147, 627, 632, 647, 813, 817, 818 |
| FatI | CATG | 4 cut(s) | 444, 576, 855, 883 |
| FauI | CCCGC | 1 cut(s) | 654 |
| FblI | GTMKAC | 1 cut(s) | 604 |
| GsuI | CTGGAG | 1 cut(s) | 36 |
| HaeIII | GGCC | 2 cut(s) | 463, 506 |
| Hin1II | CATG | 4 cut(s) | 448, 580, 859, 887 |
| HincII | GTYRAC | 2 cut(s) | 91, 126 |
| HindII | GTYRAC | 2 cut(s) | 91, 126 |
| HinfI | GANTC | 5 cut(s) | 213, 302, 358, 438, 709 |
| HphI | GGTGA | 2 cut(s) | 736, 737 |
| Hpy166II | GTNNAC | 4 cut(s) | 91, 126, 605, 792 |
| Hpy188III | TCNNGA | 6 cut(s) | 206, 217, 236, 422, 445, 545 |
| Hpy8I | GTNNAC | 4 cut(s) | 91, 126, 605, 792 |
| Hpy99I | CGWCG | 1 cut(s) | 487 |
| HpyAV | CCTTC | 1 cut(s) | 404 |
| HpyCH4IV | ACGT | 4 cut(s) | 118, 371, 482, 646 |
| HpyCH4V | TGCA | 2 cut(s) | 344, 887 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 350 |
| HpyF3I | CTNAG | 4 cut(s) | 317, 423, 543, 588 |
| HpySE526I | ACGT | 4 cut(s) | 118, 371, 482, 646 |
| Hsp92II | CATG | 4 cut(s) | 448, 580, 859, 887 |
| KpnI | GGTACC | 1 cut(s) | 853 |
| Kzo9I | GATC | 3 cut(s) | 413, 527, 552 |
| LmnI | GCTCC | 2 cut(s) | 55, 572 |
| MaeII | ACGT | 4 cut(s) | 118, 371, 482, 646 |
| MaeIII | GTNAC | 2 cut(s) | 78, 647 |
| MalI | GATC | 3 cut(s) | 415, 529, 554 |
| MbiI | CCGCTC | 1 cut(s) | 661 |
| MboI | GATC | 3 cut(s) | 413, 527, 552 |
| MboII | GAAGA | 1 cut(s) | 85 |
| MflI | RGATCY | 3 cut(s) | 413, 527, 552 |
| MluCI | AATT | 6 cut(s) | 32, 84, 363, 682, 740, 905 |
| MlyI | GAGTC | 1 cut(s) | 718 |
| MmeI | TCCRAC | 3 cut(s) | 447, 468, 503 |
| MseI | TTAA | 5 cut(s) | 8, 62, 104, 176, 348 |
| MslI | CAYNNNNRTG | 1 cut(s) | 699 |
| MspR9I | CCNGG | 2 cut(s) | 405, 718 |
| Mva1269I | GAATGC | 1 cut(s) | 815 |
| MvaI | CCWGG | 2 cut(s) | 405, 718 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 350 |
| NdeII | GATC | 3 cut(s) | 413, 527, 552 |
| NlaIII | CATG | 4 cut(s) | 448, 580, 859, 887 |
| NlaIV | GGNNCC | 6 cut(s) | 51, 415, 529, 554, 851, 861 |
| NmeAIII | GCCGAG | 1 cut(s) | 270 |
| NmuCI | GTSAC | 1 cut(s) | 78 |
| NspI | RCATGY | 1 cut(s) | 580 |
| OliI | CACNNNNGTG | 1 cut(s) | 699 |
| PagI | TCATGA | 1 cut(s) | 444 |
| PciI | ACATGT | 1 cut(s) | 576 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 778 |
| PctI | GAATGC | 1 cut(s) | 815 |
| PfeI | GAWTC | 4 cut(s) | 213, 302, 358, 438 |
| PfoI | TCCNGGA | 1 cut(s) | 403 |
| PleI | GAGTC | 1 cut(s) | 717 |
| PpsI | GAGTC | 1 cut(s) | 717 |
| PscI | ACATGT | 1 cut(s) | 576 |
| PsiI | TTATAA | 1 cut(s) | 681 |
| Psp6I | CCWGG | 2 cut(s) | 403, 716 |
| PspGI | CCWGG | 2 cut(s) | 403, 716 |
| PspN4I | GGNNCC | 6 cut(s) | 51, 415, 529, 554, 851, 861 |
| PspPI | GGNCC | 2 cut(s) | 461, 505 |
| PsuI | RGATCY | 3 cut(s) | 413, 527, 552 |
| RsaI | GTAC | 4 cut(s) | 584, 793, 841, 851 |
| RsaNI | GTAC | 4 cut(s) | 583, 792, 840, 850 |
| RseI | CAYNNNNRTG | 1 cut(s) | 699 |
| SaqAI | TTAA | 5 cut(s) | 8, 62, 104, 176, 348 |
| Sau3AI | GATC | 3 cut(s) | 413, 527, 552 |
| Sau96I | GGNCC | 2 cut(s) | 461, 505 |
| SchI | GAGTC | 1 cut(s) | 718 |
| ScrFI | CCNGG | 2 cut(s) | 405, 718 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 699 |
| Sse9I | AATT | 6 cut(s) | 32, 84, 363, 682, 740, 905 |
| SsiI | CCGC | 1 cut(s) | 661 |
| StyD4I | CCNGG | 2 cut(s) | 403, 716 |
| TaiI | ACGT | 4 cut(s) | 121, 374, 485, 649 |
| TaqI | TCGA | 2 cut(s) | 237, 356 |
| TasI | AATT | 6 cut(s) | 32, 84, 363, 682, 740, 905 |
| TatI | WGTACW | 1 cut(s) | 582 |
| TfiI | GAWTC | 4 cut(s) | 213, 302, 358, 438 |
| Tru1I | TTAA | 5 cut(s) | 8, 62, 104, 176, 348 |
| Tru9I | TTAA | 5 cut(s) | 8, 62, 104, 176, 348 |
| TscAI | CASTG | 1 cut(s) | 60 |
| TseFI | GTSAC | 1 cut(s) | 78 |
| Tsp45I | GTSAC | 1 cut(s) | 78 |
| TspDTI | ATGAA | 3 cut(s) | 179, 264, 270 |
| TspGWI | ACGGA | 5 cut(s) | 636, 641, 666, 815, 821 |
| TspRI | CASTG | 1 cut(s) | 60 |
| XapI | RAATTY | 1 cut(s) | 740 |
| XceI | RCATGY | 1 cut(s) | 580 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 843 |
| XmiI | GTMKAC | 1 cut(s) | 604 |