pycom17g27460

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0000990
Physical Location & Seq
Reverse (-)
25629091 .. 25629860
770 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 606 bp
ATGGATTACTTTAAGGCAACCTGGTATCCTAGAATTGGGATGTGGATTTCTGCACTACAAAATATTCCTCTTGCTAGCCAGGAAACCTCTACGGCAATGGAGTTCTACCACAACCAACTAAAGCTTAGGCTATTGAACGAGAAGAAACCTAGCGTGTACAAACGAGTTGATTGGTTGATTGATAAGCTGGGTACAAAAGTGCATTCCTACTTCTGGCTTGATGAATATTCCAAGAAGGATGATTTTGCACGATATTGGAAAGATGAGTGGGCGAGTGGTTTAACATCTTGGCGTAAAGCTTTGAAGATTCCTGTCTGCAATATTGTCATAGAAGGTACATGTGCAAAAGTTATTGAGGAGCTCGACCAAGAGAATGCTTATGTTGTTTGGAACCTTGGTTCGCAGTTTGGTATTTGCAACTGCCGTTGGGCAGAAATGGGCAACCTGTGTGAACATATACTCAAAGTTACTAATGTCTGCCGAAAAGGGTTGTCTACGCCATCTATCAGCCTATTGCAGTACCACAAGGCCTTGATTGATATGCTACACTGCCCACCTCATGATTCTTTGATTCATGATCAGGTTGTTTCTTTAGCAGTGGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

202

Amino Acids

23.37

Weight (kDa)

7.03

Isoelectric Point (pI)

32.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 494
AfaI GTAC 4 cut(s) 158, 193, 337, 521
AfiI CCNNNNNNNGG 2 cut(s) 35, 213
AflIII ACRYGT 1 cut(s) 338
AgsI TTSAA 2 cut(s) 136, 304
AjnI CCWGG 2 cut(s) 20, 78
AloI GAACNNNNNNTCC 2 cut(s) 382, 414
AluBI AGCT 4 cut(s) 124, 187, 299, 361
AluI AGCT 4 cut(s) 124, 187, 299, 361
Alw21I GWGCWC 1 cut(s) 363
AoxI GGCC 1 cut(s) 528
AsuNHI GCTAGC 1 cut(s) 74
BaeI ACNNNNGTAYC 2 cut(s) 183, 216
BanII GRGCYC 1 cut(s) 363
Bbv12I GWGCWC 1 cut(s) 363
BccI CCATC 1 cut(s) 508
BceAI ACGGC 2 cut(s) 108, 408
BciT130I CCWGG 2 cut(s) 22, 80
BciVI GTATCC 1 cut(s) 36
BclI TGATCA 1 cut(s) 577
BfaI CTAG 3 cut(s) 30, 75, 150
BfuI GTATCC 1 cut(s) 36
Bme1390I CCNGG 2 cut(s) 22, 80
BmiI GGNNCC 1 cut(s) 392
BmrFI CCNGG 2 cut(s) 22, 80
BmtI GCTAGC 1 cut(s) 78
Bpu10I CCTNAGC 1 cut(s) 125
BsaJI CCNNGG 1 cut(s) 394
Bsc4I CCNNNNNNNGG 2 cut(s) 35, 213
Bse3DI GCAATG 1 cut(s) 102
BseBI CCWGG 2 cut(s) 22, 80
BseDI CCNNGG 1 cut(s) 394
BseGI GGATG 2 cut(s) 45, 244
BseLI CCNNNNNNNGG 2 cut(s) 35, 213
BseMI GCAATG 1 cut(s) 102
BseRI GAGGAG 1 cut(s) 371
BseYI CCCAGC 1 cut(s) 187
BsgI GTGCAG 1 cut(s) 36
BshFI GGCC 1 cut(s) 530
BsiHKAI GWGCWC 1 cut(s) 363
BslI CCNNNNNNNGG 2 cut(s) 35, 213
BsmI GAATGC 2 cut(s) 202, 379
BsnI GGCC 1 cut(s) 530
Bsp1286I GDGCHC 1 cut(s) 363
Bsp1407I TGTACA 1 cut(s) 156
Bsp143I GATC 1 cut(s) 577
BspANI GGCC 1 cut(s) 530
BspHI TCATGA 2 cut(s) 559, 574
BspLI GGNNCC 1 cut(s) 392
BspOI GCTAGC 1 cut(s) 78
BsrDI GCAATG 1 cut(s) 102
BsrGI TGTACA 1 cut(s) 156
BssECI CCNNGG 1 cut(s) 394
BssMI GATC 1 cut(s) 577
BssT1I CCWWGG 1 cut(s) 394
Bst2UI CCWGG 2 cut(s) 22, 80
BstAUI TGTACA 1 cut(s) 156
BstC8I GCNNGC 1 cut(s) 76
BstDEI CTNAG 1 cut(s) 125
BstF5I GGATG 2 cut(s) 45, 244
BstKTI GATC 1 cut(s) 580
BstMBI GATC 1 cut(s) 577
BstNI CCWGG 2 cut(s) 22, 80
BstNSI RCATGY 1 cut(s) 342
BstSCI CCNGG 2 cut(s) 20, 78
BsuI GTATCC 1 cut(s) 36
BsuRI GGCC 1 cut(s) 530
BtsCI GGATG 2 cut(s) 45, 244
BtsI GCAGTG 2 cut(s) 547, 603
BtsIMutI CAGTG 2 cut(s) 547, 603
Cac8I GCNNGC 1 cut(s) 76
CciI TCATGA 2 cut(s) 559, 574
CsiI ACCWGGT 1 cut(s) 20
Csp6I GTAC 4 cut(s) 157, 192, 336, 520
CviAII CATG 3 cut(s) 339, 560, 575
CviJI RGCY 9 cut(s) 78, 124, 130, 187, 217, 299, 361, 510, 530
CviKI_1 RGCY 9 cut(s) 78, 124, 130, 187, 217, 299, 361, 510, 530
CviQI GTAC 4 cut(s) 157, 192, 336, 520
DdeI CTNAG 1 cut(s) 125
DpnI GATC 1 cut(s) 579
DpnII GATC 1 cut(s) 577
Ecl136II GAGCTC 1 cut(s) 361
Eco130I CCWWGG 1 cut(s) 394
Eco147I AGGCCT 1 cut(s) 530
Eco24I GRGCYC 1 cut(s) 363
Eco53kI GAGCTC 1 cut(s) 361
EcoICRI GAGCTC 1 cut(s) 361
EcoRII CCWGG 2 cut(s) 20, 78
EcoT14I CCWWGG 1 cut(s) 394
EcoT38I GRGCYC 1 cut(s) 363
ErhI CCWWGG 1 cut(s) 394
FaeI CATG 3 cut(s) 342, 563, 578
FaiI YATR 8 cut(s) 329, 340, 381, 456, 458, 542, 561, 576
FatI CATG 3 cut(s) 338, 559, 574
FbaI TGATCA 1 cut(s) 577
FblI GTMKAC 1 cut(s) 494
FokI GGATG 2 cut(s) 52, 251
FriOI GRGCYC 1 cut(s) 363
FspBI CTAG 3 cut(s) 30, 75, 150
GsaI CCCAGC 1 cut(s) 191
HaeIII GGCC 1 cut(s) 530
Hin1II CATG 3 cut(s) 342, 563, 578
HindIII AAGCTT 2 cut(s) 122, 297
HinfI GANTC 3 cut(s) 307, 563, 571
Hpy166II GTNNAC 4 cut(s) 157, 452, 495, 601
Hpy188III TCNNGA 2 cut(s) 560, 575
Hpy8I GTNNAC 4 cut(s) 157, 452, 495, 601
HpyAV CCTTC 2 cut(s) 229, 326
HpyCH4V TGCA 7 cut(s) 53, 202, 248, 318, 344, 417, 517
HpyF3I CTNAG 1 cut(s) 125
Hsp92II CATG 3 cut(s) 342, 563, 578
Ksp22I TGATCA 1 cut(s) 577
Kzo9I GATC 1 cut(s) 577
LmnI GCTCC 1 cut(s) 358
LpnPI CCDG 9 cut(s) 7, 34, 65, 92, 173, 199, 324, 458, 566
MabI ACCWGGT 1 cut(s) 20
MaeI CTAG 3 cut(s) 30, 75, 150
MaeIII GTNAC 1 cut(s) 466
MalI GATC 1 cut(s) 579
MboI GATC 1 cut(s) 577
MboII GAAGA 2 cut(s) 154, 316
MhlI GDGCHC 1 cut(s) 363
MluCI AATT 1 cut(s) 33
MnlI CCTC 4 cut(s) 78, 97, 349, 567
MseI TTAA 2 cut(s) 12, 281
MspR9I CCNGG 2 cut(s) 22, 80
Mva1269I GAATGC 2 cut(s) 202, 379
MvaI CCWGG 2 cut(s) 22, 80
NdeII GATC 1 cut(s) 577
NheI GCTAGC 1 cut(s) 74
NlaIII CATG 3 cut(s) 342, 563, 578
NlaIV GGNNCC 1 cut(s) 392
NspI RCATGY 1 cut(s) 342
PagI TCATGA 2 cut(s) 559, 574
PceI AGGCCT 1 cut(s) 530
PciI ACATGT 1 cut(s) 338
PctI GAATGC 2 cut(s) 202, 379
PfeI GAWTC 3 cut(s) 307, 563, 571
PscI ACATGT 1 cut(s) 338
Psp124BI GAGCTC 1 cut(s) 363
Psp6I CCWGG 2 cut(s) 20, 78
PspFI CCCAGC 1 cut(s) 187
PspGI CCWGG 2 cut(s) 20, 78
PspN4I GGNNCC 1 cut(s) 392
RsaI GTAC 4 cut(s) 158, 193, 337, 521
RsaNI GTAC 4 cut(s) 157, 192, 336, 520
SacI GAGCTC 1 cut(s) 363
SaqAI TTAA 2 cut(s) 12, 281
Sau3AI GATC 1 cut(s) 577
ScrFI CCNGG 2 cut(s) 22, 80
SduI GDGCHC 1 cut(s) 363
SexAI ACCWGGT 1 cut(s) 20
Sse9I AATT 1 cut(s) 33
SseBI AGGCCT 1 cut(s) 530
SspI AATATT 3 cut(s) 64, 227, 322
SspMI CTAG 3 cut(s) 30, 75, 150
SstI GAGCTC 1 cut(s) 363
StuI AGGCCT 1 cut(s) 530
StyD4I CCNGG 2 cut(s) 20, 78
StyI CCWWGG 1 cut(s) 394
TaqI TCGA 1 cut(s) 363
TasI AATT 1 cut(s) 33
TatI WGTACW 1 cut(s) 156
TfiI GAWTC 3 cut(s) 307, 563, 571
Tru1I TTAA 2 cut(s) 12, 281
Tru9I TTAA 2 cut(s) 12, 281
TscAI CASTG 2 cut(s) 554, 603
TspDTI ATGAA 2 cut(s) 237, 563
TspRI CASTG 2 cut(s) 554, 603
XceI RCATGY 1 cut(s) 342
XmiI GTMKAC 1 cut(s) 494
XspI CTAG 3 cut(s) 30, 75, 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.