Basic blue protein-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcuI | CTGAAG | 1 cut(s) | 236 |
| AfaI | GTAC | 1 cut(s) | 173 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 127 |
| AflII | CTTAAG | 1 cut(s) | 163 |
| AluBI | AGCT | 1 cut(s) | 125 |
| AluI | AGCT | 1 cut(s) | 125 |
| BauI | CACGAG | 1 cut(s) | 6 |
| BfaI | CTAG | 2 cut(s) | 95, 280 |
| BfrI | CTTAAG | 1 cut(s) | 163 |
| BmiI | GGNNCC | 1 cut(s) | 214 |
| BpuEI | CTTGAG | 1 cut(s) | 220 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 127 |
| Bse3DI | GCAATG | 2 cut(s) | 30, 190 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 127 |
| BseMI | GCAATG | 2 cut(s) | 30, 190 |
| BseYI | CCCAGC | 1 cut(s) | 99 |
| BslFI | GGGAC | 1 cut(s) | 116 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 127 |
| BsmFI | GGGAC | 1 cut(s) | 116 |
| Bsp143I | GATC | 1 cut(s) | 91 |
| BspLI | GGNNCC | 1 cut(s) | 214 |
| BspTI | CTTAAG | 1 cut(s) | 163 |
| BsrDI | GCAATG | 2 cut(s) | 30, 190 |
| BssMI | GATC | 1 cut(s) | 91 |
| BssSI | CACGAG | 1 cut(s) | 6 |
| Bst2BI | CACGAG | 1 cut(s) | 6 |
| Bst4CI | ACNGT | 1 cut(s) | 179 |
| BstAFI | CTTAAG | 1 cut(s) | 163 |
| BstKTI | GATC | 1 cut(s) | 94 |
| BstMBI | GATC | 1 cut(s) | 91 |
| BtsI | GCAGTG | 1 cut(s) | 238 |
| BtsIMutI | CAGTG | 2 cut(s) | 184, 238 |
| CseI | GACGC | 1 cut(s) | 197 |
| Csp6I | GTAC | 1 cut(s) | 172 |
| CviAII | CATG | 3 cut(s) | 58, 140, 149 |
| CviJI | RGCY | 4 cut(s) | 99, 125, 131, 279 |
| CviKI_1 | RGCY | 4 cut(s) | 99, 125, 131, 279 |
| CviQI | GTAC | 1 cut(s) | 172 |
| DpnI | GATC | 1 cut(s) | 93 |
| DpnII | GATC | 1 cut(s) | 91 |
| Eco32I | GATATC | 1 cut(s) | 236 |
| Eco57I | CTGAAG | 1 cut(s) | 236 |
| EcoRV | GATATC | 1 cut(s) | 236 |
| FaeI | CATG | 3 cut(s) | 61, 143, 152 |
| FaiI | YATR | 5 cut(s) | 59, 78, 141, 150, 229 |
| FaqI | GGGAC | 1 cut(s) | 116 |
| FatI | CATG | 3 cut(s) | 57, 139, 148 |
| FspBI | CTAG | 2 cut(s) | 95, 280 |
| GsaI | CCCAGC | 1 cut(s) | 103 |
| HgaI | GACGC | 1 cut(s) | 197 |
| Hin1II | CATG | 3 cut(s) | 61, 143, 152 |
| HindIII | AAGCTT | 1 cut(s) | 123 |
| HinfI | GANTC | 1 cut(s) | 9 |
| Hpy166II | GTNNAC | 1 cut(s) | 172 |
| Hpy188I | TCNGA | 2 cut(s) | 54, 86 |
| Hpy8I | GTNNAC | 1 cut(s) | 172 |
| Hpy99I | CGWCG | 1 cut(s) | 47 |
| HpyCH4III | ACNGT | 1 cut(s) | 179 |
| HpyCH4IV | ACGT | 1 cut(s) | 260 |
| HpySE526I | ACGT | 1 cut(s) | 260 |
| Hsp92II | CATG | 3 cut(s) | 61, 143, 152 |
| Kzo9I | GATC | 1 cut(s) | 91 |
| LpnPI | CCDG | 3 cut(s) | 50, 85, 195 |
| MaeI | CTAG | 2 cut(s) | 95, 280 |
| MaeII | ACGT | 1 cut(s) | 260 |
| MaeIII | GTNAC | 1 cut(s) | 10 |
| MalI | GATC | 1 cut(s) | 93 |
| MboI | GATC | 1 cut(s) | 91 |
| MluCI | AATT | 1 cut(s) | 115 |
| MlyI | GAGTC | 1 cut(s) | 18 |
| MmeI | TCCRAC | 2 cut(s) | 64, 133 |
| MseI | TTAA | 2 cut(s) | 164, 194 |
| MspCI | CTTAAG | 1 cut(s) | 163 |
| NdeII | GATC | 1 cut(s) | 91 |
| NlaIII | CATG | 3 cut(s) | 61, 143, 152 |
| NlaIV | GGNNCC | 1 cut(s) | 214 |
| NmuCI | GTSAC | 1 cut(s) | 10 |
| PleI | GAGTC | 1 cut(s) | 17 |
| PpsI | GAGTC | 1 cut(s) | 17 |
| PspFI | CCCAGC | 1 cut(s) | 99 |
| PspN4I | GGNNCC | 1 cut(s) | 214 |
| RsaI | GTAC | 1 cut(s) | 173 |
| RsaNI | GTAC | 1 cut(s) | 172 |
| SaqAI | TTAA | 2 cut(s) | 164, 194 |
| Sau3AI | GATC | 1 cut(s) | 91 |
| SchI | GAGTC | 1 cut(s) | 18 |
| SetI | ASST | 4 cut(s) | 127, 200, 218, 263 |
| SmlI | CTYRAG | 2 cut(s) | 163, 199 |
| SmoI | CTYRAG | 2 cut(s) | 163, 199 |
| Sse9I | AATT | 1 cut(s) | 115 |
| SspMI | CTAG | 2 cut(s) | 95, 280 |
| TaaI | ACNGT | 1 cut(s) | 179 |
| TaiI | ACGT | 1 cut(s) | 263 |
| TasI | AATT | 1 cut(s) | 115 |
| Tru1I | TTAA | 2 cut(s) | 164, 194 |
| Tru9I | TTAA | 2 cut(s) | 164, 194 |
| TscAI | CASTG | 2 cut(s) | 184, 245 |
| TseFI | GTSAC | 1 cut(s) | 10 |
| Tsp45I | GTSAC | 1 cut(s) | 10 |
| TspGWI | ACGGA | 2 cut(s) | 31, 120 |
| TspRI | CASTG | 2 cut(s) | 184, 245 |
| Vha464I | CTTAAG | 1 cut(s) | 163 |
| XspI | CTAG | 2 cut(s) | 95, 280 |