Basic blue protein-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclI | AACGTT | 1 cut(s) | 177 |
| AcoI | YGGCCR | 1 cut(s) | 250 |
| AcuI | CTGAAG | 1 cut(s) | 305 |
| AfaI | GTAC | 1 cut(s) | 242 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 127, 233 |
| AluBI | AGCT | 2 cut(s) | 125, 231 |
| AluI | AGCT | 2 cut(s) | 125, 231 |
| AoxI | GGCC | 1 cut(s) | 250 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 201, 234 |
| BalI | TGGCCA | 1 cut(s) | 252 |
| BauI | CACGAG | 1 cut(s) | 6 |
| BclI | TGATCA | 1 cut(s) | 193 |
| BfaI | CTAG | 3 cut(s) | 95, 200, 349 |
| BmiI | GGNNCC | 1 cut(s) | 283 |
| Bpu10I | CCTNAGC | 1 cut(s) | 232 |
| BpuEI | CTTGAG | 1 cut(s) | 289 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 127, 233 |
| Bse3DI | GCAATG | 1 cut(s) | 30 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 127, 233 |
| BseMI | GCAATG | 1 cut(s) | 30 |
| BseYI | CCCAGC | 1 cut(s) | 99 |
| BshFI | GGCC | 1 cut(s) | 252 |
| BslFI | GGGAC | 1 cut(s) | 116 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 127, 233 |
| BsmFI | GGGAC | 1 cut(s) | 116 |
| BsnI | GGCC | 1 cut(s) | 252 |
| Bsp143I | GATC | 2 cut(s) | 91, 193 |
| BspANI | GGCC | 1 cut(s) | 252 |
| BspLI | GGNNCC | 1 cut(s) | 283 |
| BsrDI | GCAATG | 1 cut(s) | 30 |
| BssMI | GATC | 2 cut(s) | 91, 193 |
| BssSI | CACGAG | 1 cut(s) | 6 |
| Bst2BI | CACGAG | 1 cut(s) | 6 |
| Bst4CI | ACNGT | 1 cut(s) | 248 |
| BstDEI | CTNAG | 1 cut(s) | 232 |
| BstKTI | GATC | 2 cut(s) | 94, 196 |
| BstMBI | GATC | 2 cut(s) | 91, 193 |
| BsuRI | GGCC | 1 cut(s) | 252 |
| BtsI | GCAGTG | 1 cut(s) | 307 |
| BtsIMutI | CAGTG | 2 cut(s) | 253, 307 |
| Csp6I | GTAC | 1 cut(s) | 241 |
| CviAII | CATG | 3 cut(s) | 58, 140, 149 |
| CviJI | RGCY | 6 cut(s) | 99, 125, 131, 191, 231, 252 |
| CviKI_1 | RGCY | 6 cut(s) | 99, 125, 131, 191, 231, 252 |
| CviQI | GTAC | 1 cut(s) | 241 |
| DdeI | CTNAG | 1 cut(s) | 232 |
| DpnI | GATC | 2 cut(s) | 93, 195 |
| DpnII | GATC | 2 cut(s) | 91, 193 |
| EaeI | YGGCCR | 1 cut(s) | 250 |
| Eco32I | GATATC | 1 cut(s) | 305 |
| Eco57I | CTGAAG | 1 cut(s) | 305 |
| EcoRV | GATATC | 1 cut(s) | 305 |
| FaeI | CATG | 3 cut(s) | 61, 143, 152 |
| FaiI | YATR | 5 cut(s) | 59, 78, 141, 150, 298 |
| FaqI | GGGAC | 1 cut(s) | 116 |
| FatI | CATG | 3 cut(s) | 57, 139, 148 |
| FbaI | TGATCA | 1 cut(s) | 193 |
| FspBI | CTAG | 3 cut(s) | 95, 200, 349 |
| GsaI | CCCAGC | 1 cut(s) | 103 |
| HaeIII | GGCC | 1 cut(s) | 252 |
| Hin1II | CATG | 3 cut(s) | 61, 143, 152 |
| HindIII | AAGCTT | 2 cut(s) | 123, 229 |
| HinfI | GANTC | 1 cut(s) | 9 |
| Hpy166II | GTNNAC | 2 cut(s) | 241, 346 |
| Hpy188I | TCNGA | 2 cut(s) | 54, 86 |
| Hpy8I | GTNNAC | 2 cut(s) | 241, 346 |
| HpyCH4III | ACNGT | 1 cut(s) | 248 |
| HpyCH4IV | ACGT | 1 cut(s) | 177 |
| HpyF3I | CTNAG | 1 cut(s) | 232 |
| HpySE526I | ACGT | 1 cut(s) | 177 |
| Hsp92II | CATG | 3 cut(s) | 61, 143, 152 |
| Ksp22I | TGATCA | 1 cut(s) | 193 |
| Kzo9I | GATC | 2 cut(s) | 91, 193 |
| LpnPI | CCDG | 3 cut(s) | 50, 85, 264 |
| MaeI | CTAG | 3 cut(s) | 95, 200, 349 |
| MaeII | ACGT | 1 cut(s) | 177 |
| MaeIII | GTNAC | 1 cut(s) | 10 |
| MalI | GATC | 2 cut(s) | 93, 195 |
| MboI | GATC | 2 cut(s) | 91, 193 |
| MlsI | TGGCCA | 1 cut(s) | 252 |
| MluCI | AATT | 1 cut(s) | 115 |
| MluNI | TGGCCA | 1 cut(s) | 252 |
| MlyI | GAGTC | 1 cut(s) | 18 |
| MmeI | TCCRAC | 2 cut(s) | 64, 133 |
| Mox20I | TGGCCA | 1 cut(s) | 252 |
| MscI | TGGCCA | 1 cut(s) | 252 |
| MseI | TTAA | 2 cut(s) | 164, 263 |
| Msp20I | TGGCCA | 1 cut(s) | 252 |
| NdeII | GATC | 2 cut(s) | 91, 193 |
| NlaIII | CATG | 3 cut(s) | 61, 143, 152 |
| NlaIV | GGNNCC | 1 cut(s) | 283 |
| NmuCI | GTSAC | 1 cut(s) | 10 |
| PleI | GAGTC | 1 cut(s) | 17 |
| PpsI | GAGTC | 1 cut(s) | 17 |
| Psp1406I | AACGTT | 1 cut(s) | 177 |
| PspFI | CCCAGC | 1 cut(s) | 99 |
| PspN4I | GGNNCC | 1 cut(s) | 283 |
| RsaI | GTAC | 1 cut(s) | 242 |
| RsaNI | GTAC | 1 cut(s) | 241 |
| SaqAI | TTAA | 2 cut(s) | 164, 263 |
| Sau3AI | GATC | 2 cut(s) | 91, 193 |
| SchI | GAGTC | 1 cut(s) | 18 |
| SetI | ASST | 6 cut(s) | 127, 180, 233, 238, 269, 287 |
| SmlI | CTYRAG | 1 cut(s) | 268 |
| SmoI | CTYRAG | 1 cut(s) | 268 |
| Sse9I | AATT | 1 cut(s) | 115 |
| SspMI | CTAG | 3 cut(s) | 95, 200, 349 |
| TaaI | ACNGT | 1 cut(s) | 248 |
| TaiI | ACGT | 1 cut(s) | 180 |
| TasI | AATT | 1 cut(s) | 115 |
| Tru1I | TTAA | 2 cut(s) | 164, 263 |
| Tru9I | TTAA | 2 cut(s) | 164, 263 |
| TscAI | CASTG | 2 cut(s) | 253, 314 |
| TseFI | GTSAC | 1 cut(s) | 10 |
| Tsp45I | GTSAC | 1 cut(s) | 10 |
| TspDTI | ATGAA | 2 cut(s) | 183, 210 |
| TspGWI | ACGGA | 2 cut(s) | 31, 120 |
| TspRI | CASTG | 2 cut(s) | 253, 314 |
| XspI | CTAG | 3 cut(s) | 95, 200, 349 |