RchiOBHm_Chr7g0239811

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001770
Physical Location & Seq
Reverse (-)
65549232 .. 65551037
1806 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21494

Sequence Viewer

Length: 471 bp
ATGGCGTATTTCCAGAACGATGACCTCGAGTACGTCGTCAACGACTACTACGGCGACGTCGTTGAGTTTGAGGAAGACAACGACGCTTTTGCTGATAACCAGCCCCGGCGAAGCCTTGAGGTCGTAGACTCCGACTCCGACTCCGACTCCGACGACGACTTTGAAGACGACCTTGATTCGAGCCAGGTGAAGAGTGATACATCGGCTTTGGAAGCTAGAAATGGAAAGGATATACAGGGAATACCATGGGAGAGGCTTAATTTCACAAGAGATAAGTACCGTGAGGCGCGATTGAAGCAGTATAAGAACTATGAGAGCCTCGGTCGGCCTCGTCAAGACCTCGAGAAGGAGTGCTTGCAAGTGGAGAAGGGGAAGAGGTTCTATGACTTTCACTACAACACAAGGCTTGTGAAGTCGACAATTGTGCATTTTCAGGTAGAGATCATGATCTTGGTTCCCGTAACTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

156

Amino Acids

18.4

Weight (kDa)

4.41

Isoelectric Point (pI)

42.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 60
AccI GTMKAC 2 cut(s) 126, 416
AccII CGCG 1 cut(s) 289
AcyI GRCGYC 1 cut(s) 57
AfaI GTAC 2 cut(s) 32, 278
AfiI CCNNNNNNNGG 1 cut(s) 346
AgsI TTSAA 2 cut(s) 164, 295
AjnI CCWGG 1 cut(s) 183
AluBI AGCT 1 cut(s) 215
AluI AGCT 1 cut(s) 215
Ama87I CYCGRG 2 cut(s) 26, 341
AoxI GGCC 1 cut(s) 326
ArsI GACNNNNNNTTYG 2 cut(s) 143, 175
Asp700I GAANNNNTTC 1 cut(s) 377
AspLEI GCGC 1 cut(s) 289
AsuC2I CCSGG 1 cut(s) 106
AsuHPI GGTGA 1 cut(s) 199
AvaI CYCGRG 2 cut(s) 26, 341
BbsI GAAGAC 2 cut(s) 81, 171
BceAI ACGGC 1 cut(s) 67
BcgI CGANNNNNNTGC 4 cut(s) 71, 105, 406, 440
BciT130I CCWGG 1 cut(s) 185
BcnI CCSGG 1 cut(s) 106
BfaI CTAG 1 cut(s) 216
Bme1390I CCNGG 2 cut(s) 106, 185
BmeT110I CYCGRG 2 cut(s) 26, 341
BmiI GGNNCC 1 cut(s) 456
BmrFI CCNGG 2 cut(s) 106, 185
BpiI GAAGAC 2 cut(s) 81, 171
BpuEI CTTGAG 1 cut(s) 137
BpuMI CCSGG 1 cut(s) 106
BsaBI GATNNNNATC 1 cut(s) 446
BsaHI GRCGYC 1 cut(s) 57
BsaJI CCNNGG 3 cut(s) 104, 245, 319
BsaXI ACNNNNNCTCC 6 cut(s) 119, 125, 131, 149, 155, 161
Bsc4I CCNNNNNNNGG 1 cut(s) 346
Bse8I GATNNNNATC 1 cut(s) 446
BseBI CCWGG 1 cut(s) 185
BseDI CCNNGG 3 cut(s) 104, 245, 319
BseJI GATNNNNATC 1 cut(s) 446
BseLI CCNNNNNNNGG 1 cut(s) 346
Bsh1236I CGCG 1 cut(s) 289
Bsh1285I CGRYCG 1 cut(s) 325
BshFI GGCC 1 cut(s) 328
BsiEI CGRYCG 1 cut(s) 325
BsiHKCI CYCGRG 2 cut(s) 26, 341
BsiSI CCGG 1 cut(s) 106
BslI CCNNNNNNNGG 1 cut(s) 346
BsnI GGCC 1 cut(s) 328
BsoBI CYCGRG 2 cut(s) 26, 341
Bsp143I GATC 2 cut(s) 441, 447
Bsp19I CCATGG 1 cut(s) 245
BspANI GGCC 1 cut(s) 328
BspFNI CGCG 1 cut(s) 289
BspHI TCATGA 1 cut(s) 444
BspLI GGNNCC 1 cut(s) 456
BssECI CCNNGG 3 cut(s) 104, 245, 319
BssMI GATC 2 cut(s) 441, 447
BssNI GRCGYC 1 cut(s) 57
BssT1I CCWWGG 1 cut(s) 245
Bst2UI CCWGG 1 cut(s) 185
Bst4CI ACNGT 2 cut(s) 281, 466
Bst6I CTCTTC 2 cut(s) 185, 368
BstACI GRCGYC 1 cut(s) 57
BstC8I GCNNGC 1 cut(s) 356
BstDSI CCRYGG 1 cut(s) 245
BstENI CCTNNNNNAGG 1 cut(s) 344
BstFNI CGCG 1 cut(s) 289
BstHHI GCGC 1 cut(s) 289
BstKTI GATC 2 cut(s) 444, 450
BstMBI GATC 2 cut(s) 441, 447
BstMCI CGRYCG 1 cut(s) 325
BstMWI GCNNNNNNNGC 2 cut(s) 212, 295
BstNI CCWGG 1 cut(s) 185
BstSCI CCNGG 2 cut(s) 104, 183
BstUI CGCG 1 cut(s) 289
BstV2I GAAGAC 2 cut(s) 81, 171
BsuRI GGCC 1 cut(s) 328
BtgI CCRYGG 1 cut(s) 245
Cac8I GCNNGC 1 cut(s) 356
CciI TCATGA 1 cut(s) 444
CfoI GCGC 1 cut(s) 289
CseI GACGC 1 cut(s) 92
Csp6I GTAC 2 cut(s) 31, 277
CviAII CATG 2 cut(s) 246, 445
CviJI RGCY 9 cut(s) 103, 114, 183, 206, 215, 256, 318, 328, 406
CviKI_1 RGCY 9 cut(s) 103, 114, 183, 206, 215, 256, 318, 328, 406
CviQI GTAC 2 cut(s) 31, 277
DpnI GATC 2 cut(s) 443, 449
DpnII GATC 2 cut(s) 441, 447
Eam1104I CTCTTC 2 cut(s) 185, 368
EarI CTCTTC 2 cut(s) 185, 368
Eco130I CCWWGG 1 cut(s) 245
Eco88I CYCGRG 2 cut(s) 26, 341
EcoNI CCTNNNNNAGG 1 cut(s) 344
EcoRII CCWGG 1 cut(s) 183
EcoT14I CCWWGG 1 cut(s) 245
ErhI CCWWGG 1 cut(s) 245
FaeI CATG 2 cut(s) 249, 448
FaiI YATR 6 cut(s) 233, 247, 303, 312, 384, 446
FalI AAGNNNNNCTT 4 cut(s) 156, 188, 338, 370
FatI CATG 2 cut(s) 245, 444
FblI GTMKAC 2 cut(s) 126, 416
FspBI CTAG 1 cut(s) 216
GlaI GCGC 1 cut(s) 288
HaeIII GGCC 1 cut(s) 328
HapII CCGG 1 cut(s) 106
HgaI GACGC 1 cut(s) 92
HhaI GCGC 1 cut(s) 289
Hin1I GRCGYC 1 cut(s) 57
Hin1II CATG 2 cut(s) 249, 448
Hin6I GCGC 1 cut(s) 287
HinP1I GCGC 1 cut(s) 287
HincII GTYRAC 2 cut(s) 40, 417
HindII GTYRAC 2 cut(s) 40, 417
HinfI GANTC 5 cut(s) 128, 134, 140, 146, 176
HpaII CCGG 1 cut(s) 106
HphI GGTGA 1 cut(s) 199
Hpy166II GTNNAC 3 cut(s) 40, 127, 417
Hpy188I TCNGA 4 cut(s) 133, 139, 145, 151
Hpy188III TCNNGA 4 cut(s) 13, 335, 343, 445
Hpy8I GTNNAC 3 cut(s) 40, 127, 417
Hpy99I CGWCG 6 cut(s) 38, 59, 62, 86, 155, 158
HpyAV CCTTC 2 cut(s) 340, 361
HpyCH4III ACNGT 2 cut(s) 281, 466
HpyCH4IV ACGT 2 cut(s) 33, 57
HpyCH4V TGCA 2 cut(s) 358, 427
HpyF10VI GCNNNNNNNGC 2 cut(s) 212, 295
HpySE526I ACGT 2 cut(s) 33, 57
Hsp92I GRCGYC 1 cut(s) 57
Hsp92II CATG 2 cut(s) 249, 448
HspAI GCGC 1 cut(s) 287
Kzo9I GATC 2 cut(s) 441, 447
LpnPI CCDG 7 cut(s) 26, 113, 119, 170, 197, 221, 419
MaeI CTAG 1 cut(s) 216
MaeII ACGT 2 cut(s) 33, 57
MaeIII GTNAC 1 cut(s) 460
MalI GATC 2 cut(s) 443, 449
MboI GATC 2 cut(s) 441, 447
MboII GAAGA 4 cut(s) 86, 176, 202, 385
MfeI CAATTG 1 cut(s) 420
MluCI AATT 2 cut(s) 259, 420
MlyI GAGTC 4 cut(s) 122, 128, 134, 140
MmeI TCCRAC 4 cut(s) 156, 162, 168, 174
MnlI CCTC 9 cut(s) 35, 64, 112, 246, 277, 329, 339, 350, 369
MroXI GAANNNNTTC 1 cut(s) 377
MseI TTAA 1 cut(s) 258
MspI CCGG 1 cut(s) 106
MspR9I CCNGG 2 cut(s) 106, 185
MunI CAATTG 1 cut(s) 420
MvaI CCWGG 1 cut(s) 185
MvnI CGCG 1 cut(s) 289
MwoI GCNNNNNNNGC 2 cut(s) 212, 295
NciI CCSGG 1 cut(s) 106
NcoI CCATGG 1 cut(s) 245
NdeII GATC 2 cut(s) 441, 447
NlaIII CATG 2 cut(s) 249, 448
NlaIV GGNNCC 1 cut(s) 456
PaeR7I CTCGAG 2 cut(s) 26, 341
PagI TCATGA 1 cut(s) 444
PcsI WCGNNNNNNNCGW 5 cut(s) 24, 33, 39, 57, 129
PdmI GAANNNNTTC 1 cut(s) 377
PfeI GAWTC 1 cut(s) 176
PleI GAGTC 4 cut(s) 122, 128, 134, 140
PpsI GAGTC 4 cut(s) 122, 128, 134, 140
Psp6I CCWGG 1 cut(s) 183
PspGI CCWGG 1 cut(s) 183
PspN4I GGNNCC 1 cut(s) 456
PspXI VCTCGAGB 1 cut(s) 26
RsaI GTAC 2 cut(s) 32, 278
RsaNI GTAC 2 cut(s) 31, 277
SalI GTCGAC 1 cut(s) 415
SaqAI TTAA 1 cut(s) 258
Sau3AI GATC 2 cut(s) 441, 447
SchI GAGTC 4 cut(s) 122, 128, 134, 140
ScrFI CCNGG 2 cut(s) 106, 185
Sfr274I CTCGAG 2 cut(s) 26, 341
SlaI CTCGAG 2 cut(s) 26, 341
SmlI CTYRAG 3 cut(s) 26, 116, 341
SmoI CTYRAG 3 cut(s) 26, 116, 341
Sse9I AATT 2 cut(s) 259, 420
SspMI CTAG 1 cut(s) 216
StyD4I CCNGG 2 cut(s) 104, 183
StyI CCWWGG 1 cut(s) 245
TaaI ACNGT 2 cut(s) 281, 466
TaiI ACGT 2 cut(s) 36, 60
TaqI TCGA 4 cut(s) 27, 179, 342, 416
TaqII GACCGA 1 cut(s) 311
TasI AATT 2 cut(s) 259, 420
TfiI GAWTC 1 cut(s) 176
Tru1I TTAA 1 cut(s) 258
Tru9I TTAA 1 cut(s) 258
XagI CCTNNNNNAGG 1 cut(s) 344
XhoI CTCGAG 2 cut(s) 26, 341
XmiI GTMKAC 2 cut(s) 126, 416
XmnI GAANNNNTTC 1 cut(s) 377
XspI CTAG 1 cut(s) 216
ZraI GACGTC 1 cut(s) 58
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.