RLG00000001720

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001849
Physical Location & Seq
Forward (+)
19580757 .. 19581220
464 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001720

Sequence Viewer

Length: 363 bp
ATGGACCTCACCGAGCTCGCACCCACCGTGGCCATGCTCGCTGGCAACGCGAGATCGACGCGCGTGAACCTCTCAACACAGCCGCTCACGCACTTGGAAGAGCGTGACGGCAGAGCGCACCCAAGAGTCGACGTGGGAGGTGTAGTACTGGTAGAAGATGAAAGAGAGGCGGAAGGTTTTGATTGGAGAATTGTGGCGGAGAATGAGAGCGCCGTTGACGAACTCGGCGAAGCCGTGGCTCTTGATGGGGAGGGGTTCGGCTTAGTTGACCGGGAAGAGGTGGTAGGAGAAGTCGAGGAAGGGGAGACGGGACCAGAGGGATCGCCATTGGCGGGAGACGAGGGAGTTGTGGATGGCCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

121

Amino Acids

12.68

Weight (kDa)

4.05

Isoelectric Point (pI)

23.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 85
AccI GTMKAC 1 cut(s) 129
AccII CGCG 3 cut(s) 50, 61, 63
AciI CCGC 4 cut(s) 83, 170, 197, 332
AclWI GGATC 1 cut(s) 328
AcoI YGGCCR 1 cut(s) 30
AfaI GTAC 1 cut(s) 147
AfiI CCNNNNNNNGG 2 cut(s) 277, 332
AjiI CACGTC 1 cut(s) 133
AluBI AGCT 1 cut(s) 16
AluI AGCT 1 cut(s) 16
Alw21I GWGCWC 1 cut(s) 18
Alw26I GTCTC 2 cut(s) 299, 330
AlwI GGATC 1 cut(s) 328
AoxI GGCC 2 cut(s) 30, 355
AspLEI GCGC 3 cut(s) 63, 118, 212
AspS9I GGNCC 2 cut(s) 4, 311
AsuC2I CCSGG 1 cut(s) 272
AvaII GGWCC 2 cut(s) 4, 311
BalI TGGCCA 1 cut(s) 32
BanII GRGCYC 1 cut(s) 18
Bbv12I GWGCWC 1 cut(s) 18
BccI CCATC 2 cut(s) 239, 347
BceAI ACGGC 3 cut(s) 124, 197, 218
BcnI CCSGG 1 cut(s) 272
BcoDI GTCTC 2 cut(s) 299, 330
BfoI RGCGCY 1 cut(s) 213
BisI GCNGC 1 cut(s) 83
BlsI GCNGC 1 cut(s) 84
BmcAI AGTACT 1 cut(s) 147
Bme1390I CCNGG 1 cut(s) 272
Bme18I GGWCC 2 cut(s) 4, 311
BmgBI CACGTC 1 cut(s) 133
BmgT120I GGNCC 2 cut(s) 4, 311
BmiI GGNNCC 1 cut(s) 312
BmrFI CCNGG 1 cut(s) 272
BpuMI CCSGG 1 cut(s) 272
BsaJI CCNNGG 2 cut(s) 27, 234
BsaXI ACNNNNNCTCC 2 cut(s) 129, 159
Bsc4I CCNNNNNNNGG 2 cut(s) 277, 332
Bse1I ACTGG 1 cut(s) 153
BseDI CCNNGG 2 cut(s) 27, 234
BseGI GGATG 1 cut(s) 358
BseLI CCNNNNNNNGG 2 cut(s) 277, 332
BseNI ACTGG 1 cut(s) 153
Bsh1236I CGCG 3 cut(s) 50, 61, 63
BshFI GGCC 2 cut(s) 32, 357
BsiHKAI GWGCWC 1 cut(s) 18
BsiSI CCGG 1 cut(s) 271
BslFI GGGAC 1 cut(s) 324
BslI CCNNNNNNNGG 2 cut(s) 277, 332
BsmAI GTCTC 2 cut(s) 299, 330
BsmBI CGTCTC 2 cut(s) 299, 330
BsmFI GGGAC 1 cut(s) 324
BsnI GGCC 2 cut(s) 32, 357
Bsp1286I GDGCHC 1 cut(s) 18
Bsp143I GATC 2 cut(s) 53, 320
BspACI CCGC 4 cut(s) 83, 170, 197, 332
BspANI GGCC 2 cut(s) 32, 357
BspFNI CGCG 3 cut(s) 50, 61, 63
BspLI GGNNCC 1 cut(s) 312
BspPI GGATC 1 cut(s) 328
BspQI GCTCTTC 1 cut(s) 93
BsrBI CCGCTC 1 cut(s) 85
BsrI ACTGG 1 cut(s) 153
BssECI CCNNGG 2 cut(s) 27, 234
BssMI GATC 2 cut(s) 53, 320
Bst4CI ACNGT 1 cut(s) 28
Bst6I CTCTTC 2 cut(s) 93, 270
BstC8I GCNNGC 3 cut(s) 18, 39, 43
BstDEI CTNAG 1 cut(s) 262
BstDSI CCRYGG 2 cut(s) 27, 234
BstF5I GGATG 1 cut(s) 358
BstFNI CGCG 3 cut(s) 50, 61, 63
BstH2I RGCGCY 1 cut(s) 213
BstHHI GCGC 3 cut(s) 63, 118, 212
BstKTI GATC 2 cut(s) 56, 323
BstMAI GTCTC 2 cut(s) 299, 330
BstMBI GATC 2 cut(s) 53, 320
BstMWI GCNNNNNNNGC 3 cut(s) 38, 47, 88
BstSCI CCNGG 1 cut(s) 270
BstUI CGCG 3 cut(s) 50, 61, 63
BsuRI GGCC 2 cut(s) 32, 357
BtgI CCRYGG 2 cut(s) 27, 234
BtrI CACGTC 1 cut(s) 133
BtsCI GGATG 1 cut(s) 358
Cac8I GCNNGC 3 cut(s) 18, 39, 43
CfoI GCGC 3 cut(s) 63, 118, 212
Cfr13I GGNCC 2 cut(s) 4, 311
CseI GACGC 1 cut(s) 67
Csp6I GTAC 1 cut(s) 146
CviAII CATG 1 cut(s) 34
CviJI RGCY 7 cut(s) 16, 32, 82, 233, 239, 261, 357
CviKI_1 RGCY 7 cut(s) 16, 32, 82, 233, 239, 261, 357
CviQI GTAC 1 cut(s) 146
DdeI CTNAG 1 cut(s) 262
DpnI GATC 2 cut(s) 55, 322
DpnII GATC 2 cut(s) 53, 320
EaeI YGGCCR 1 cut(s) 30
Eam1104I CTCTTC 2 cut(s) 93, 270
EarI CTCTTC 2 cut(s) 93, 270
EciI GGCGGA 2 cut(s) 185, 212
Ecl136II GAGCTC 1 cut(s) 16
Eco24I GRGCYC 1 cut(s) 18
Eco47I GGWCC 2 cut(s) 4, 311
Eco53kI GAGCTC 1 cut(s) 16
EcoICRI GAGCTC 1 cut(s) 16
EcoT38I GRGCYC 1 cut(s) 18
Esp3I CGTCTC 2 cut(s) 299, 330
FaeI CATG 1 cut(s) 37
FaiI YATR 1 cut(s) 35
FaqI GGGAC 1 cut(s) 324
FatI CATG 1 cut(s) 33
FauI CCCGC 1 cut(s) 325
FblI GTMKAC 1 cut(s) 129
Fnu4HI GCNGC 1 cut(s) 83
FriOI GRGCYC 1 cut(s) 18
Fsp4HI GCNGC 1 cut(s) 83
GlaI GCGC 3 cut(s) 62, 117, 211
GluI GCNGC 1 cut(s) 83
HaeII RGCGCY 1 cut(s) 213
HaeIII GGCC 2 cut(s) 32, 357
HapII CCGG 1 cut(s) 271
HgaI GACGC 1 cut(s) 67
HhaI GCGC 3 cut(s) 63, 118, 212
Hin1II CATG 1 cut(s) 37
Hin6I GCGC 3 cut(s) 61, 116, 210
HinP1I GCGC 3 cut(s) 61, 116, 210
HincII GTYRAC 3 cut(s) 130, 217, 268
HindII GTYRAC 3 cut(s) 130, 217, 268
HinfI GANTC 1 cut(s) 126
HpaII CCGG 1 cut(s) 271
Hpy166II GTNNAC 4 cut(s) 67, 130, 217, 268
Hpy188III TCNNGA 1 cut(s) 242
Hpy8I GTNNAC 4 cut(s) 67, 130, 217, 268
Hpy99I CGWCG 2 cut(s) 61, 134
HpyAV CCTTC 2 cut(s) 167, 293
HpyCH4III ACNGT 1 cut(s) 28
HpyCH4IV ACGT 1 cut(s) 132
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 47, 88
HpyF3I CTNAG 1 cut(s) 262
HpySE526I ACGT 1 cut(s) 132
Hsp92II CATG 1 cut(s) 37
HspAI GCGC 3 cut(s) 61, 116, 210
Kzo9I GATC 2 cut(s) 53, 320
LguI GCTCTTC 1 cut(s) 93
LpnPI CCDG 4 cut(s) 27, 134, 284, 327
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 1 cut(s) 104
MalI GATC 2 cut(s) 55, 322
MbiI CCGCTC 1 cut(s) 85
MboI GATC 2 cut(s) 53, 320
MboII GAAGA 3 cut(s) 110, 167, 287
MhlI GDGCHC 1 cut(s) 18
MlsI TGGCCA 1 cut(s) 32
MluCI AATT 1 cut(s) 189
MluNI TGGCCA 1 cut(s) 32
MlyI GAGTC 1 cut(s) 135
MnlI CCTC 9 cut(s) 17, 80, 131, 160, 244, 271, 289, 310, 334
Mox20I TGGCCA 1 cut(s) 32
MscI TGGCCA 1 cut(s) 32
Msp20I TGGCCA 1 cut(s) 32
MspI CCGG 1 cut(s) 271
MspR9I CCNGG 1 cut(s) 272
MvnI CGCG 3 cut(s) 50, 61, 63
MwoI GCNNNNNNNGC 3 cut(s) 38, 47, 88
NciI CCSGG 1 cut(s) 272
NdeII GATC 2 cut(s) 53, 320
NlaIII CATG 1 cut(s) 37
NlaIV GGNNCC 1 cut(s) 312
NmeAIII GCCGAG 1 cut(s) 204
NmuCI GTSAC 1 cut(s) 104
PciSI GCTCTTC 1 cut(s) 93
PcsI WCGNNNNNNNCGW 3 cut(s) 24, 225, 231
PkrI GCNGC 1 cut(s) 84
PleI GAGTC 1 cut(s) 134
PpsI GAGTC 1 cut(s) 134
Psp124BI GAGCTC 1 cut(s) 18
PspN4I GGNNCC 1 cut(s) 312
PspPI GGNCC 2 cut(s) 4, 311
RsaI GTAC 1 cut(s) 147
RsaNI GTAC 1 cut(s) 146
SacI GAGCTC 1 cut(s) 18
SalI GTCGAC 1 cut(s) 128
SapI GCTCTTC 1 cut(s) 93
SatI GCNGC 1 cut(s) 83
Sau3AI GATC 2 cut(s) 53, 320
Sau96I GGNCC 2 cut(s) 4, 311
ScaI AGTACT 1 cut(s) 147
SchI GAGTC 1 cut(s) 135
ScrFI CCNGG 1 cut(s) 272
SduI GDGCHC 1 cut(s) 18
SetI ASST 7 cut(s) 9, 18, 72, 135, 142, 178, 282
SinI GGWCC 2 cut(s) 4, 311
Sse9I AATT 1 cut(s) 189
SsiI CCGC 4 cut(s) 83, 170, 197, 332
SstI GAGCTC 1 cut(s) 18
StyD4I CCNGG 1 cut(s) 270
TaaI ACNGT 1 cut(s) 28
TaiI ACGT 1 cut(s) 135
TaqI TCGA 3 cut(s) 56, 129, 294
TasI AATT 1 cut(s) 189
TatI WGTACW 1 cut(s) 145
TauI GCSGC 1 cut(s) 85
TseFI GTSAC 1 cut(s) 104
Tsp45I GTSAC 1 cut(s) 104
TspDTI ATGAA 1 cut(s) 174
VpaK11BI GGWCC 2 cut(s) 4, 311
XmiI GTMKAC 1 cut(s) 129
ZrmI AGTACT 1 cut(s) 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.