RLG00000018970

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001849
Physical Location & Seq
Reverse (-)
43810217 .. 43810680
464 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018970

Sequence Viewer

Length: 378 bp
ATGGACCTCACCGAGCTCACACCTACTGTGGCCATGCTCGCCGGCAACTCGAGATCGACCCGCATGGTACTCTTTGTCGATGAAGAAATCGAGTTCAGGCTCACGCACCTGGAAGCGCGTGACGGCAGAGCGCACTCAAGAGTCAACGTAGGAGGTGTAGTACTGGTAGAAGAGGAAAGAGAGGCGGAAGGTTTTGATTGGAGAATTGGGGCGGAGAATGAGAGCACGGTTGACGAACTCGCCGAAGCCGTGGCTCGTGATGGGGAGGGGTTCGGCGTCATTGACCAAGAAGAAGTGGTAGGAGAACTCGAGGAAGGGGAGACGGGACCAGAGGGATCGCCATTAGCGGGAGACGAGGGAGTTGTGGATGGCCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

126

Amino Acids

13.47

Weight (kDa)

4.06

Isoelectric Point (pI)

36.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 118
AciI CCGC 4 cut(s) 61, 185, 212, 347
AclWI GGATC 1 cut(s) 343
AcoI YGGCCR 1 cut(s) 30
AcyI GRCGYC 1 cut(s) 276
AfaI GTAC 2 cut(s) 69, 162
AfiI CCNNNNNNNGG 1 cut(s) 347
AjnI CCWGG 1 cut(s) 108
AluBI AGCT 1 cut(s) 16
AluI AGCT 1 cut(s) 16
Alw21I GWGCWC 2 cut(s) 18, 227
Alw26I GTCTC 2 cut(s) 314, 345
AlwI GGATC 1 cut(s) 343
Ama87I CYCGRG 2 cut(s) 49, 308
AoxI GGCC 2 cut(s) 30, 370
AspLEI GCGC 2 cut(s) 118, 133
AspS9I GGNCC 2 cut(s) 4, 326
AvaI CYCGRG 2 cut(s) 49, 308
AvaII GGWCC 2 cut(s) 4, 326
BalI TGGCCA 1 cut(s) 32
BanII GRGCYC 1 cut(s) 18
BauI CACGAG 1 cut(s) 255
Bbv12I GWGCWC 2 cut(s) 18, 227
BccI CCATC 2 cut(s) 254, 362
BceAI ACGGC 2 cut(s) 139, 233
BciT130I CCWGG 1 cut(s) 110
BcoDI GTCTC 2 cut(s) 314, 345
BmcAI AGTACT 1 cut(s) 162
Bme1390I CCNGG 1 cut(s) 110
Bme18I GGWCC 2 cut(s) 4, 326
BmeT110I CYCGRG 2 cut(s) 49, 308
BmgT120I GGNCC 2 cut(s) 4, 326
BmiI GGNNCC 1 cut(s) 327
BmrFI CCNGG 1 cut(s) 110
BpuEI CTTGAG 1 cut(s) 121
BsaHI GRCGYC 1 cut(s) 276
BsaJI CCNNGG 1 cut(s) 249
BsaXI ACNNNNNCTCC 2 cut(s) 144, 174
Bsc4I CCNNNNNNNGG 1 cut(s) 347
Bse118I RCCGGY 1 cut(s) 41
Bse1I ACTGG 1 cut(s) 168
BseBI CCWGG 1 cut(s) 110
BseDI CCNNGG 1 cut(s) 249
BseGI GGATG 1 cut(s) 373
BseLI CCNNNNNNNGG 1 cut(s) 347
BseNI ACTGG 1 cut(s) 168
Bsh1236I CGCG 1 cut(s) 118
BshFI GGCC 2 cut(s) 32, 372
BsiHKAI GWGCWC 2 cut(s) 18, 227
BsiHKCI CYCGRG 2 cut(s) 49, 308
BsiSI CCGG 1 cut(s) 42
BslFI GGGAC 1 cut(s) 339
BslI CCNNNNNNNGG 1 cut(s) 347
BsmAI GTCTC 2 cut(s) 314, 345
BsmBI CGTCTC 2 cut(s) 314, 345
BsmFI GGGAC 1 cut(s) 339
BsnI GGCC 2 cut(s) 32, 372
BsoBI CYCGRG 2 cut(s) 49, 308
Bsp1286I GDGCHC 2 cut(s) 18, 227
Bsp143I GATC 2 cut(s) 53, 335
BspACI CCGC 4 cut(s) 61, 185, 212, 347
BspANI GGCC 2 cut(s) 32, 372
BspFNI CGCG 1 cut(s) 118
BspLI GGNNCC 1 cut(s) 327
BspPI GGATC 1 cut(s) 343
BsrFI RCCGGY 1 cut(s) 41
BsrI ACTGG 1 cut(s) 168
BssAI RCCGGY 1 cut(s) 41
BssECI CCNNGG 1 cut(s) 249
BssMI GATC 2 cut(s) 53, 335
BssNI GRCGYC 1 cut(s) 276
BssSI CACGAG 1 cut(s) 255
Bst2BI CACGAG 1 cut(s) 255
Bst2UI CCWGG 1 cut(s) 110
Bst4CI ACNGT 2 cut(s) 28, 229
Bst6I CTCTTC 1 cut(s) 165
BstACI GRCGYC 1 cut(s) 276
BstC8I GCNNGC 2 cut(s) 39, 43
BstDSI CCRYGG 1 cut(s) 249
BstF5I GGATG 1 cut(s) 373
BstFNI CGCG 1 cut(s) 118
BstHHI GCGC 2 cut(s) 118, 133
BstKTI GATC 2 cut(s) 56, 338
BstMAI GTCTC 2 cut(s) 314, 345
BstMBI GATC 2 cut(s) 53, 335
BstMWI GCNNNNNNNGC 1 cut(s) 38
BstNI CCWGG 1 cut(s) 110
BstSCI CCNGG 1 cut(s) 108
BstUI CGCG 1 cut(s) 118
BsuRI GGCC 2 cut(s) 32, 372
BtgI CCRYGG 1 cut(s) 249
BtsCI GGATG 1 cut(s) 373
Cac8I GCNNGC 2 cut(s) 39, 43
CfoI GCGC 2 cut(s) 118, 133
Cfr10I RCCGGY 1 cut(s) 41
Cfr13I GGNCC 2 cut(s) 4, 326
CseI GACGC 1 cut(s) 265
Csp6I GTAC 2 cut(s) 68, 161
CviAII CATG 2 cut(s) 34, 64
CviJI RGCY 6 cut(s) 16, 32, 100, 248, 254, 372
CviKI_1 RGCY 6 cut(s) 16, 32, 100, 248, 254, 372
CviQI GTAC 2 cut(s) 68, 161
DpnI GATC 2 cut(s) 55, 337
DpnII GATC 2 cut(s) 53, 335
EaeI YGGCCR 1 cut(s) 30
Eam1104I CTCTTC 1 cut(s) 165
EarI CTCTTC 1 cut(s) 165
EciI GGCGGA 2 cut(s) 200, 227
Ecl136II GAGCTC 1 cut(s) 16
Eco24I GRGCYC 1 cut(s) 18
Eco47I GGWCC 2 cut(s) 4, 326
Eco53kI GAGCTC 1 cut(s) 16
Eco88I CYCGRG 2 cut(s) 49, 308
EcoICRI GAGCTC 1 cut(s) 16
EcoRII CCWGG 1 cut(s) 108
EcoT38I GRGCYC 1 cut(s) 18
Esp3I CGTCTC 2 cut(s) 314, 345
FaeI CATG 2 cut(s) 37, 67
FaiI YATR 2 cut(s) 35, 65
FaqI GGGAC 1 cut(s) 339
FatI CATG 2 cut(s) 33, 63
FauI CCCGC 2 cut(s) 68, 340
FriOI GRGCYC 1 cut(s) 18
GlaI GCGC 2 cut(s) 117, 132
HaeIII GGCC 2 cut(s) 32, 372
HapII CCGG 1 cut(s) 42
HgaI GACGC 1 cut(s) 265
HhaI GCGC 2 cut(s) 118, 133
Hin1I GRCGYC 1 cut(s) 276
Hin1II CATG 2 cut(s) 37, 67
Hin6I GCGC 2 cut(s) 116, 131
HinP1I GCGC 2 cut(s) 116, 131
HincII GTYRAC 2 cut(s) 145, 232
HindII GTYRAC 2 cut(s) 145, 232
HinfI GANTC 1 cut(s) 141
HpaII CCGG 1 cut(s) 42
Hpy166II GTNNAC 2 cut(s) 145, 232
Hpy188III TCNNGA 3 cut(s) 51, 138, 257
Hpy8I GTNNAC 2 cut(s) 145, 232
HpyAV CCTTC 2 cut(s) 182, 308
HpyCH4III ACNGT 2 cut(s) 28, 229
HpyCH4IV ACGT 1 cut(s) 147
HpyF10VI GCNNNNNNNGC 1 cut(s) 38
HpySE526I ACGT 1 cut(s) 147
Hsp92I GRCGYC 1 cut(s) 276
Hsp92II CATG 2 cut(s) 37, 67
HspAI GCGC 2 cut(s) 116, 131
KroI GCCGGC 1 cut(s) 41
KroNI GCCGGC 1 cut(s) 43
Kzo9I GATC 2 cut(s) 53, 335
LpnPI CCDG 6 cut(s) 55, 82, 95, 122, 149, 342
MaeII ACGT 1 cut(s) 147
MaeIII GTNAC 1 cut(s) 119
MalI GATC 2 cut(s) 55, 337
MboI GATC 2 cut(s) 53, 335
MboII GAAGA 3 cut(s) 95, 182, 302
MhlI GDGCHC 2 cut(s) 18, 227
MlsI TGGCCA 1 cut(s) 32
MluCI AATT 1 cut(s) 204
MluNI TGGCCA 1 cut(s) 32
MlyI GAGTC 1 cut(s) 150
MnlI CCTC 8 cut(s) 17, 146, 166, 175, 259, 304, 325, 349
Mox20I TGGCCA 1 cut(s) 32
MroNI GCCGGC 1 cut(s) 41
MscI TGGCCA 1 cut(s) 32
Msp20I TGGCCA 1 cut(s) 32
MspI CCGG 1 cut(s) 42
MspR9I CCNGG 1 cut(s) 110
MvaI CCWGG 1 cut(s) 110
MvnI CGCG 1 cut(s) 118
MwoI GCNNNNNNNGC 1 cut(s) 38
NaeI GCCGGC 1 cut(s) 43
NdeII GATC 2 cut(s) 53, 335
NgoMIV GCCGGC 1 cut(s) 41
NlaIII CATG 2 cut(s) 37, 67
NlaIV GGNNCC 1 cut(s) 327
NmuCI GTSAC 1 cut(s) 119
PaeR7I CTCGAG 2 cut(s) 49, 308
PcsI WCGNNNNNNNCGW 2 cut(s) 240, 246
PdiI GCCGGC 1 cut(s) 43
PleI GAGTC 1 cut(s) 149
PpsI GAGTC 1 cut(s) 149
Psp124BI GAGCTC 1 cut(s) 18
Psp6I CCWGG 1 cut(s) 108
PspGI CCWGG 1 cut(s) 108
PspN4I GGNNCC 1 cut(s) 327
PspPI GGNCC 2 cut(s) 4, 326
PspXI VCTCGAGB 1 cut(s) 308
RsaI GTAC 2 cut(s) 69, 162
RsaNI GTAC 2 cut(s) 68, 161
SacI GAGCTC 1 cut(s) 18
Sau3AI GATC 2 cut(s) 53, 335
Sau96I GGNCC 2 cut(s) 4, 326
ScaI AGTACT 1 cut(s) 162
SchI GAGTC 1 cut(s) 150
ScrFI CCNGG 1 cut(s) 110
SduI GDGCHC 2 cut(s) 18, 227
SetI ASST 7 cut(s) 9, 18, 25, 111, 150, 157, 193
Sfr274I CTCGAG 2 cut(s) 49, 308
SinI GGWCC 2 cut(s) 4, 326
SlaI CTCGAG 2 cut(s) 49, 308
SmlI CTYRAG 3 cut(s) 49, 136, 308
SmoI CTYRAG 3 cut(s) 49, 136, 308
Sse9I AATT 1 cut(s) 204
SsiI CCGC 4 cut(s) 61, 185, 212, 347
SstI GAGCTC 1 cut(s) 18
StyD4I CCNGG 1 cut(s) 108
TaaI ACNGT 2 cut(s) 28, 229
TaiI ACGT 1 cut(s) 150
TaqI TCGA 5 cut(s) 50, 56, 78, 90, 309
TasI AATT 1 cut(s) 204
TatI WGTACW 1 cut(s) 160
TseFI GTSAC 1 cut(s) 119
Tsp45I GTSAC 1 cut(s) 119
TspDTI ATGAA 1 cut(s) 96
VpaK11BI GGWCC 2 cut(s) 4, 326
XhoI CTCGAG 2 cut(s) 49, 308
ZrmI AGTACT 1 cut(s) 162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.