RLG00000018028

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001849
Physical Location & Seq
Forward (+)
24600113 .. 24601869
1757 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018028

Sequence Viewer

Length: 381 bp
ATGGTGCTCTTTGTTGATGAAGAAATCAAGGTCAGGCTCATGCACCTGGAAGCGCGTGACGGCAGAGCGCACCTAAGAGTCGACGTGGGAGGTGTAGTACTGGTAGAAGATGAAAGAGAGGCGGAAGGTTTTGATTGGAGAATTGGGGCGGAGAATGAGAGCGCGGTTGACGAACTCGGCGAAGCCGTGGCTCGTGATGGGGAGGGGTTCGGCGTCGTTGACCTGGAAGAGGTGGTAGGAGATGGGACCAGAGGGATCGCCATTGGCGGGAGACGAGGGAGTTGTGGATGGCCTCTACGTATTCCTGCACTTAGGATTCTTTTGATAATTCCTAATAGTAAGGAGCAGTATTCCCTCACTAAAAGTATTTCAGGCATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

127

Amino Acids

13.74

Weight (kDa)

4.7

Isoelectric Point (pI)

30.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 81
AccII CGCG 2 cut(s) 55, 164
AciI CCGC 4 cut(s) 122, 149, 164, 267
AclWI GGATC 1 cut(s) 263
AcyI GRCGYC 1 cut(s) 213
AfaI GTAC 1 cut(s) 99
AfiI CCNNNNNNNGG 2 cut(s) 229, 267
AjiI CACGTC 1 cut(s) 85
AjnI CCWGG 2 cut(s) 45, 222
Alw21I GWGCWC 1 cut(s) 9
Alw26I GTCTC 1 cut(s) 265
AlwI GGATC 1 cut(s) 263
AoxI GGCC 1 cut(s) 290
AspLEI GCGC 3 cut(s) 55, 70, 164
AspS9I GGNCC 1 cut(s) 246
AvaII GGWCC 1 cut(s) 246
BauI CACGAG 1 cut(s) 192
Bbv12I GWGCWC 1 cut(s) 9
BccI CCATC 3 cut(s) 191, 236, 282
BceAI ACGGC 2 cut(s) 76, 170
BciT130I CCWGG 2 cut(s) 47, 224
BcoDI GTCTC 1 cut(s) 265
BmcAI AGTACT 1 cut(s) 99
Bme1390I CCNGG 2 cut(s) 47, 224
Bme18I GGWCC 1 cut(s) 246
BmgBI CACGTC 1 cut(s) 85
BmgT120I GGNCC 1 cut(s) 246
BmiI GGNNCC 1 cut(s) 247
BmrFI CCNGG 2 cut(s) 47, 224
BsaAI YACGTR 1 cut(s) 299
BsaHI GRCGYC 1 cut(s) 213
BsaJI CCNNGG 1 cut(s) 186
BsaXI ACNNNNNCTCC 2 cut(s) 81, 111
Bsc4I CCNNNNNNNGG 2 cut(s) 229, 267
Bse1I ACTGG 1 cut(s) 105
BseBI CCWGG 2 cut(s) 47, 224
BseDI CCNNGG 1 cut(s) 186
BseGI GGATG 1 cut(s) 293
BseLI CCNNNNNNNGG 2 cut(s) 229, 267
BseNI ACTGG 1 cut(s) 105
BsgI GTGCAG 1 cut(s) 291
Bsh1236I CGCG 2 cut(s) 55, 164
BshFI GGCC 1 cut(s) 292
BsiHKAI GWGCWC 1 cut(s) 9
BslFI GGGAC 1 cut(s) 259
BslI CCNNNNNNNGG 2 cut(s) 229, 267
BsmAI GTCTC 1 cut(s) 265
BsmBI CGTCTC 1 cut(s) 265
BsmFI GGGAC 1 cut(s) 259
BsnI GGCC 1 cut(s) 292
Bsp1286I GDGCHC 1 cut(s) 9
Bsp143I GATC 1 cut(s) 255
BspACI CCGC 4 cut(s) 122, 149, 164, 267
BspANI GGCC 1 cut(s) 292
BspFNI CGCG 2 cut(s) 55, 164
BspLI GGNNCC 1 cut(s) 247
BspPI GGATC 1 cut(s) 263
BsrI ACTGG 1 cut(s) 105
BssECI CCNNGG 1 cut(s) 186
BssMI GATC 1 cut(s) 255
BssNI GRCGYC 1 cut(s) 213
BssSI CACGAG 1 cut(s) 192
Bst2BI CACGAG 1 cut(s) 192
Bst2UI CCWGG 2 cut(s) 47, 224
Bst6I CTCTTC 1 cut(s) 222
BstACI GRCGYC 1 cut(s) 213
BstBAI YACGTR 1 cut(s) 299
BstDEI CTNAG 2 cut(s) 74, 311
BstDSI CCRYGG 1 cut(s) 186
BstENI CCTNNNNNAGG 1 cut(s) 227
BstF5I GGATG 1 cut(s) 293
BstFNI CGCG 2 cut(s) 55, 164
BstHHI GCGC 3 cut(s) 55, 70, 164
BstKTI GATC 1 cut(s) 258
BstMAI GTCTC 1 cut(s) 265
BstMBI GATC 1 cut(s) 255
BstNI CCWGG 2 cut(s) 47, 224
BstNSI RCATGY 1 cut(s) 379
BstSCI CCNGG 2 cut(s) 45, 222
BstSNI TACGTA 1 cut(s) 299
BstUI CGCG 2 cut(s) 55, 164
BsuRI GGCC 1 cut(s) 292
BtgI CCRYGG 1 cut(s) 186
BtrI CACGTC 1 cut(s) 85
BtsCI GGATG 1 cut(s) 293
CfoI GCGC 3 cut(s) 55, 70, 164
Cfr13I GGNCC 1 cut(s) 246
CseI GACGC 1 cut(s) 202
Csp6I GTAC 1 cut(s) 98
CviAII CATG 2 cut(s) 40, 376
CviJI RGCY 4 cut(s) 37, 185, 191, 292
CviKI_1 RGCY 4 cut(s) 37, 185, 191, 292
CviQI GTAC 1 cut(s) 98
DdeI CTNAG 2 cut(s) 74, 311
DpnI GATC 1 cut(s) 257
DpnII GATC 1 cut(s) 255
Eam1104I CTCTTC 1 cut(s) 222
EarI CTCTTC 1 cut(s) 222
EciI GGCGGA 2 cut(s) 137, 164
Eco105I TACGTA 1 cut(s) 299
Eco47I GGWCC 1 cut(s) 246
EcoNI CCTNNNNNAGG 1 cut(s) 227
EcoRII CCWGG 2 cut(s) 45, 222
Esp3I CGTCTC 1 cut(s) 265
FaeI CATG 2 cut(s) 43, 379
FaiI YATR 2 cut(s) 41, 377
FaqI GGGAC 1 cut(s) 259
FatI CATG 2 cut(s) 39, 375
FauI CCCGC 1 cut(s) 260
FblI GTMKAC 1 cut(s) 81
FokI GGATG 1 cut(s) 300
GlaI GCGC 3 cut(s) 54, 69, 163
HaeIII GGCC 1 cut(s) 292
HgaI GACGC 1 cut(s) 202
HhaI GCGC 3 cut(s) 55, 70, 164
Hin1I GRCGYC 1 cut(s) 213
Hin1II CATG 2 cut(s) 43, 379
Hin6I GCGC 3 cut(s) 53, 68, 162
HinP1I GCGC 3 cut(s) 53, 68, 162
HincII GTYRAC 3 cut(s) 82, 169, 220
HindII GTYRAC 3 cut(s) 82, 169, 220
HinfI GANTC 2 cut(s) 78, 316
Hpy166II GTNNAC 3 cut(s) 82, 169, 220
Hpy188III TCNNGA 1 cut(s) 194
Hpy8I GTNNAC 3 cut(s) 82, 169, 220
Hpy99I CGWCG 2 cut(s) 86, 218
HpyAV CCTTC 1 cut(s) 119
HpyCH4IV ACGT 2 cut(s) 84, 298
HpyCH4V TGCA 2 cut(s) 43, 308
HpyF3I CTNAG 2 cut(s) 74, 311
HpySE526I ACGT 2 cut(s) 84, 298
Hsp92I GRCGYC 1 cut(s) 213
Hsp92II CATG 2 cut(s) 43, 379
HspAI GCGC 3 cut(s) 53, 68, 162
Kzo9I GATC 1 cut(s) 255
LmnI GCTCC 1 cut(s) 343
LpnPI CCDG 9 cut(s) 19, 32, 59, 86, 209, 236, 262, 318, 357
MaeII ACGT 2 cut(s) 84, 298
MaeIII GTNAC 1 cut(s) 56
MalI GATC 1 cut(s) 257
MboI GATC 1 cut(s) 255
MboII GAAGA 3 cut(s) 32, 119, 239
MhlI GDGCHC 1 cut(s) 9
MluCI AATT 2 cut(s) 141, 327
MlyI GAGTC 1 cut(s) 87
MnlI CCTC 8 cut(s) 83, 112, 196, 223, 245, 269, 303, 365
MspR9I CCNGG 2 cut(s) 47, 224
MvaI CCWGG 2 cut(s) 47, 224
MvnI CGCG 2 cut(s) 55, 164
NdeII GATC 1 cut(s) 255
NlaIII CATG 2 cut(s) 43, 379
NlaIV GGNNCC 1 cut(s) 247
NmeAIII GCCGAG 1 cut(s) 156
NmuCI GTSAC 1 cut(s) 56
NspI RCATGY 1 cut(s) 379
PcsI WCGNNNNNNNCGW 2 cut(s) 177, 183
PfeI GAWTC 1 cut(s) 316
PleI GAGTC 1 cut(s) 86
PpsI GAGTC 1 cut(s) 86
Ppu21I YACGTR 1 cut(s) 299
Psp6I CCWGG 2 cut(s) 45, 222
PspGI CCWGG 2 cut(s) 45, 222
PspN4I GGNNCC 1 cut(s) 247
PspPI GGNCC 1 cut(s) 246
RsaI GTAC 1 cut(s) 99
RsaNI GTAC 1 cut(s) 98
SalI GTCGAC 1 cut(s) 80
Sau3AI GATC 1 cut(s) 255
Sau96I GGNCC 1 cut(s) 246
ScaI AGTACT 1 cut(s) 99
SchI GAGTC 1 cut(s) 87
ScrFI CCNGG 2 cut(s) 47, 224
SduI GDGCHC 1 cut(s) 9
SetI ASST 9 cut(s) 33, 48, 75, 87, 94, 130, 225, 234, 301
SinI GGWCC 1 cut(s) 246
SnaBI TACGTA 1 cut(s) 299
Sse9I AATT 2 cut(s) 141, 327
SsiI CCGC 4 cut(s) 122, 149, 164, 267
StyD4I CCNGG 2 cut(s) 45, 222
TaiI ACGT 2 cut(s) 87, 301
TaqI TCGA 1 cut(s) 81
TasI AATT 2 cut(s) 141, 327
TatI WGTACW 1 cut(s) 97
TfiI GAWTC 1 cut(s) 316
TseFI GTSAC 1 cut(s) 56
Tsp45I GTSAC 1 cut(s) 56
TspDTI ATGAA 2 cut(s) 33, 126
VpaK11BI GGWCC 1 cut(s) 246
XagI CCTNNNNNAGG 1 cut(s) 227
XceI RCATGY 1 cut(s) 379
XmiI GTMKAC 1 cut(s) 81
ZrmI AGTACT 1 cut(s) 99
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.