RLG00000029184

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001849
Physical Location & Seq
Forward (+)
34182305 .. 34182768
464 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029184

Sequence Viewer

Length: 363 bp
ATGGACCTCACCAAGCTCACACCCACCATGGCCATGCTAGCTTGCAACGCTAGATCGACGCGCGTGAGCCTCTCAACACAGCGGCTCACTCACCTGGAAGCGCGTGACAGCAGAGCACACCTAAGAGTCGACGTGGGAGGTGTAGTACTGGTAGAAGATGAAAGAGAGGCGGAAGGTTTTGATTGGAGAATTGGGGAGGAGAATGAGAGCGCGATTGACGAACTCGACGAAGCTGTGGCTCGTGATGGGGAGGGGTTCGGCGTCATTGACCTGGAAGAGGTGGTAGAAGAAGTCAAGGAAGGGGAGACGGGACCAGAGGGATCGCCATTGGCGGGAGACGAGGGAGTTGTGGATGGCCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

121

Amino Acids

12.97

Weight (kDa)

4.19

Isoelectric Point (pI)

33.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 129
AccII CGCG 4 cut(s) 61, 63, 103, 212
AciI CCGC 3 cut(s) 82, 170, 332
AclWI GGATC 1 cut(s) 328
AcoI YGGCCR 1 cut(s) 30
AcyI GRCGYC 1 cut(s) 261
AfaI GTAC 1 cut(s) 147
AfiI CCNNNNNNNGG 2 cut(s) 277, 332
AjiI CACGTC 1 cut(s) 133
AjnI CCWGG 2 cut(s) 93, 270
AluBI AGCT 3 cut(s) 16, 41, 233
AluI AGCT 3 cut(s) 16, 41, 233
Alw21I GWGCWC 1 cut(s) 118
Alw26I GTCTC 2 cut(s) 299, 330
AlwI GGATC 1 cut(s) 328
AoxI GGCC 2 cut(s) 30, 355
AspLEI GCGC 3 cut(s) 63, 103, 212
AspS9I GGNCC 2 cut(s) 4, 311
AsuHPI GGTGA 1 cut(s) 83
AsuNHI GCTAGC 1 cut(s) 37
AvaII GGWCC 2 cut(s) 4, 311
BalI TGGCCA 1 cut(s) 32
BauI CACGAG 1 cut(s) 240
Bbv12I GWGCWC 1 cut(s) 118
BccI CCATC 2 cut(s) 239, 347
BciT130I CCWGG 2 cut(s) 95, 272
BcoDI GTCTC 2 cut(s) 299, 330
BfaI CTAG 2 cut(s) 38, 51
BisI GCNGC 1 cut(s) 83
BlsI GCNGC 1 cut(s) 84
BmcAI AGTACT 1 cut(s) 147
Bme1390I CCNGG 2 cut(s) 95, 272
Bme18I GGWCC 2 cut(s) 4, 311
BmgBI CACGTC 1 cut(s) 133
BmgT120I GGNCC 2 cut(s) 4, 311
BmiI GGNNCC 1 cut(s) 312
BmrFI CCNGG 2 cut(s) 95, 272
BmtI GCTAGC 1 cut(s) 41
BsaHI GRCGYC 1 cut(s) 261
BsaJI CCNNGG 1 cut(s) 27
BsaXI ACNNNNNCTCC 2 cut(s) 129, 159
Bsc4I CCNNNNNNNGG 2 cut(s) 277, 332
Bse1I ACTGG 1 cut(s) 153
BseBI CCWGG 2 cut(s) 95, 272
BseDI CCNNGG 1 cut(s) 27
BseGI GGATG 1 cut(s) 358
BseLI CCNNNNNNNGG 2 cut(s) 277, 332
BseNI ACTGG 1 cut(s) 153
BseRI GAGGAG 1 cut(s) 212
Bsh1236I CGCG 4 cut(s) 61, 63, 103, 212
BshFI GGCC 2 cut(s) 32, 357
BsiHKAI GWGCWC 1 cut(s) 118
BslFI GGGAC 1 cut(s) 324
BslI CCNNNNNNNGG 2 cut(s) 277, 332
BsmAI GTCTC 2 cut(s) 299, 330
BsmBI CGTCTC 2 cut(s) 299, 330
BsmFI GGGAC 1 cut(s) 324
BsnI GGCC 2 cut(s) 32, 357
Bsp1286I GDGCHC 1 cut(s) 118
Bsp143I GATC 2 cut(s) 53, 320
Bsp19I CCATGG 1 cut(s) 27
BspACI CCGC 3 cut(s) 82, 170, 332
BspANI GGCC 2 cut(s) 32, 357
BspFNI CGCG 4 cut(s) 61, 63, 103, 212
BspLI GGNNCC 1 cut(s) 312
BspOI GCTAGC 1 cut(s) 41
BspPI GGATC 1 cut(s) 328
BsrI ACTGG 1 cut(s) 153
BssECI CCNNGG 1 cut(s) 27
BssMI GATC 2 cut(s) 53, 320
BssNI GRCGYC 1 cut(s) 261
BssSI CACGAG 1 cut(s) 240
BssT1I CCWWGG 1 cut(s) 27
Bst2BI CACGAG 1 cut(s) 240
Bst2UI CCWGG 2 cut(s) 95, 272
Bst6I CTCTTC 1 cut(s) 270
BstACI GRCGYC 1 cut(s) 261
BstC8I GCNNGC 2 cut(s) 39, 43
BstDEI CTNAG 1 cut(s) 122
BstDSI CCRYGG 1 cut(s) 27
BstENI CCTNNNNNAGG 1 cut(s) 275
BstF5I GGATG 1 cut(s) 358
BstFNI CGCG 4 cut(s) 61, 63, 103, 212
BstHHI GCGC 3 cut(s) 63, 103, 212
BstKTI GATC 2 cut(s) 56, 323
BstMAI GTCTC 2 cut(s) 299, 330
BstMBI GATC 2 cut(s) 53, 320
BstMWI GCNNNNNNNGC 2 cut(s) 38, 47
BstNI CCWGG 2 cut(s) 95, 272
BstSCI CCNGG 2 cut(s) 93, 270
BstUI CGCG 4 cut(s) 61, 63, 103, 212
BsuRI GGCC 2 cut(s) 32, 357
BtgI CCRYGG 1 cut(s) 27
BtrI CACGTC 1 cut(s) 133
BtsCI GGATG 1 cut(s) 358
Cac8I GCNNGC 2 cut(s) 39, 43
CfoI GCGC 3 cut(s) 63, 103, 212
Cfr13I GGNCC 2 cut(s) 4, 311
CseI GACGC 2 cut(s) 67, 250
Csp6I GTAC 1 cut(s) 146
CviAII CATG 2 cut(s) 28, 34
CviJI RGCY 8 cut(s) 16, 32, 41, 69, 85, 233, 239, 357
CviKI_1 RGCY 8 cut(s) 16, 32, 41, 69, 85, 233, 239, 357
CviQI GTAC 1 cut(s) 146
DdeI CTNAG 1 cut(s) 122
DpnI GATC 2 cut(s) 55, 322
DpnII GATC 2 cut(s) 53, 320
EaeI YGGCCR 1 cut(s) 30
Eam1104I CTCTTC 1 cut(s) 270
EarI CTCTTC 1 cut(s) 270
EciI GGCGGA 1 cut(s) 185
Eco130I CCWWGG 1 cut(s) 27
Eco47I GGWCC 2 cut(s) 4, 311
EcoNI CCTNNNNNAGG 1 cut(s) 275
EcoRII CCWGG 2 cut(s) 93, 270
EcoT14I CCWWGG 1 cut(s) 27
ErhI CCWWGG 1 cut(s) 27
Esp3I CGTCTC 2 cut(s) 299, 330
FaeI CATG 2 cut(s) 31, 37
FaiI YATR 2 cut(s) 29, 35
FaqI GGGAC 1 cut(s) 324
FatI CATG 2 cut(s) 27, 33
FauI CCCGC 1 cut(s) 325
FblI GTMKAC 1 cut(s) 129
Fnu4HI GCNGC 1 cut(s) 83
Fsp4HI GCNGC 1 cut(s) 83
FspBI CTAG 2 cut(s) 38, 51
GlaI GCGC 3 cut(s) 62, 102, 211
GluI GCNGC 1 cut(s) 83
HaeIII GGCC 2 cut(s) 32, 357
HgaI GACGC 2 cut(s) 67, 250
HhaI GCGC 3 cut(s) 63, 103, 212
Hin1I GRCGYC 1 cut(s) 261
Hin1II CATG 2 cut(s) 31, 37
Hin6I GCGC 3 cut(s) 61, 101, 210
HinP1I GCGC 3 cut(s) 61, 101, 210
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HinfI GANTC 1 cut(s) 126
HphI GGTGA 1 cut(s) 83
Hpy166II GTNNAC 1 cut(s) 130
Hpy188I TCNGA 1 cut(s) 362
Hpy188III TCNNGA 1 cut(s) 242
Hpy8I GTNNAC 1 cut(s) 130
Hpy99I CGWCG 3 cut(s) 61, 134, 230
HpyAV CCTTC 2 cut(s) 167, 293
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 1 cut(s) 45
HpyF10VI GCNNNNNNNGC 2 cut(s) 38, 47
HpyF3I CTNAG 1 cut(s) 122
HpySE526I ACGT 1 cut(s) 132
Hsp92I GRCGYC 1 cut(s) 261
Hsp92II CATG 2 cut(s) 31, 37
HspAI GCGC 3 cut(s) 61, 101, 210
Kzo9I GATC 2 cut(s) 53, 320
LpnPI CCDG 6 cut(s) 80, 107, 134, 257, 284, 327
MaeI CTAG 2 cut(s) 38, 51
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 1 cut(s) 104
MalI GATC 2 cut(s) 55, 322
MboI GATC 2 cut(s) 53, 320
MboII GAAGA 3 cut(s) 167, 287, 299
MhlI GDGCHC 1 cut(s) 118
MlsI TGGCCA 1 cut(s) 32
MluCI AATT 1 cut(s) 189
MluNI TGGCCA 1 cut(s) 32
MlyI GAGTC 1 cut(s) 135
MnlI CCTC 9 cut(s) 17, 80, 131, 160, 190, 244, 271, 310, 334
Mox20I TGGCCA 1 cut(s) 32
MscI TGGCCA 1 cut(s) 32
MslI CAYNNNNRTG 1 cut(s) 32
Msp20I TGGCCA 1 cut(s) 32
MspA1I CMGCKG 1 cut(s) 82
MspR9I CCNGG 2 cut(s) 95, 272
MvaI CCWGG 2 cut(s) 95, 272
MvnI CGCG 4 cut(s) 61, 63, 103, 212
MwoI GCNNNNNNNGC 2 cut(s) 38, 47
NcoI CCATGG 1 cut(s) 27
NdeII GATC 2 cut(s) 53, 320
NheI GCTAGC 1 cut(s) 37
NlaIII CATG 2 cut(s) 31, 37
NlaIV GGNNCC 1 cut(s) 312
NmuCI GTSAC 1 cut(s) 104
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PkrI GCNGC 1 cut(s) 84
PleI GAGTC 1 cut(s) 134
PpsI GAGTC 1 cut(s) 134
Psp6I CCWGG 2 cut(s) 93, 270
PspGI CCWGG 2 cut(s) 93, 270
PspN4I GGNNCC 1 cut(s) 312
PspPI GGNCC 2 cut(s) 4, 311
RsaI GTAC 1 cut(s) 147
RsaNI GTAC 1 cut(s) 146
RseI CAYNNNNRTG 1 cut(s) 32
SalI GTCGAC 1 cut(s) 128
SatI GCNGC 1 cut(s) 83
Sau3AI GATC 2 cut(s) 53, 320
Sau96I GGNCC 2 cut(s) 4, 311
ScaI AGTACT 1 cut(s) 147
SchI GAGTC 1 cut(s) 135
ScrFI CCNGG 2 cut(s) 95, 272
SduI GDGCHC 1 cut(s) 118
SinI GGWCC 2 cut(s) 4, 311
SmiMI CAYNNNNRTG 1 cut(s) 32
Sse9I AATT 1 cut(s) 189
SsiI CCGC 3 cut(s) 82, 170, 332
SspMI CTAG 2 cut(s) 38, 51
StyD4I CCNGG 2 cut(s) 93, 270
StyI CCWWGG 1 cut(s) 27
TaiI ACGT 1 cut(s) 135
TaqI TCGA 3 cut(s) 56, 129, 225
TasI AATT 1 cut(s) 189
TatI WGTACW 1 cut(s) 145
TauI GCSGC 1 cut(s) 85
TseFI GTSAC 1 cut(s) 104
Tsp45I GTSAC 1 cut(s) 104
TspDTI ATGAA 1 cut(s) 174
VpaK11BI GGWCC 2 cut(s) 4, 311
XagI CCTNNNNNAGG 1 cut(s) 275
XmiI GTMKAC 1 cut(s) 129
XspI CTAG 2 cut(s) 38, 51
ZrmI AGTACT 1 cut(s) 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.