RLG00000022600

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0001849
Physical Location & Seq
Reverse (-)
4918114 .. 4920966
2853 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022600

Sequence Viewer

Length: 714 bp
ATGCGTGGACCAGAACTTAAACCATTGAGGGTTTCAACCTTCAAGGGCAATGCCCAGAATGATAAATCTGGAGGTAGAAAAGGTGGATCAAAGCTTCCTAAGAATTTGGTCAAACTAAAAGAGAATGAGGATACCAAAGTGGGATATCCCCAGGCAAATGATATCCTTATTCTTATGCCTCTGAAGCAAATGAGACCCCAAAATCAAACGCTCCCACATCTGGTCCATCAAATCCACATCCTCAAGAAACATGGACCTCACCGAGCTCACACCCACTGTGGCCATTCTAGCCCGCAACACGAGATCGACGCGCGTGAGCCTCTCAACACAGCCGTTTCTCAGCACAGAGAAAGGGACATCATACTTGTCACGCGGAGCTCGATCTTCCTCGCTCCAGAGATGGTACTTTTTGTCGATGAAGAAATCGAGGTCAGGCTCACGCACCTGGAAGCGCGTGACGGCAGAGCGCACCCAAGAGTCGACATGGGAGGTGTAGTACTGGTAGAAGATGAAAGAGAGGCGGAAGGTTTTGATTGGAGAATTGGGGCGGAGAATGAGAGTGCGGTTGACGAACTCGGCGAAGCCGTGGCTTGTGATGGGGAGGGGTTCGGCATCGTTGACCGGGAAGAGGTGGTAGGAGAAGTCGAGGAGGGGGAGACGGGACCAGAGGGATCCCCATTGGCGGGAGATGAGGGAGTTGTGGATGGTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

238

Amino Acids

26.09

Weight (kDa)

5.24

Isoelectric Point (pI)

34.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 480
AccII CGCG 4 cut(s) 311, 313, 373, 454
AciI CCGC 6 cut(s) 293, 373, 521, 548, 563, 683
AclWI GGATC 3 cut(s) 94, 666, 679
AcoI YGGCCR 1 cut(s) 280
AcsI RAATTY 1 cut(s) 103
AcuI CTGAAG 1 cut(s) 203
AfaI GTAC 2 cut(s) 405, 498
AfiI CCNNNNNNNGG 4 cut(s) 220, 628, 682, 683
AgsI TTSAA 2 cut(s) 36, 43
AjnI CCWGG 2 cut(s) 150, 444
AluBI AGCT 3 cut(s) 94, 266, 378
AluI AGCT 3 cut(s) 94, 266, 378
Alw21I GWGCWC 2 cut(s) 268, 380
Alw26I GTCTC 2 cut(s) 187, 650
AlwI GGATC 3 cut(s) 94, 666, 679
AoxI GGCC 1 cut(s) 280
ApoI RAATTY 1 cut(s) 103
AspLEI GCGC 3 cut(s) 313, 454, 469
AspS9I GGNCC 4 cut(s) 8, 223, 254, 662
AsuC2I CCSGG 1 cut(s) 623
AsuHPI GGTGA 1 cut(s) 251
AvaII GGWCC 4 cut(s) 8, 223, 254, 662
BaeI ACNNNNGTAYC 2 cut(s) 123, 156
BalI TGGCCA 1 cut(s) 282
BamHI GGATCC 1 cut(s) 671
BanII GRGCYC 2 cut(s) 268, 380
BauI CACGAG 1 cut(s) 299
Bbv12I GWGCWC 2 cut(s) 268, 380
BccI CCATC 4 cut(s) 234, 394, 590, 698
BceAI ACGGC 3 cut(s) 317, 475, 569
BciT130I CCWGG 2 cut(s) 152, 446
BciVI GTATCC 1 cut(s) 124
BcnI CCSGG 1 cut(s) 623
BcoDI GTCTC 2 cut(s) 187, 650
BfaI CTAG 1 cut(s) 288
BfuI GTATCC 1 cut(s) 124
BmcAI AGTACT 1 cut(s) 498
Bme1390I CCNGG 3 cut(s) 152, 446, 623
Bme18I GGWCC 4 cut(s) 8, 223, 254, 662
BmgT120I GGNCC 4 cut(s) 8, 223, 254, 662
BmiI GGNNCC 2 cut(s) 663, 673
BmrFI CCNGG 3 cut(s) 152, 446, 623
BmsI GCATC 1 cut(s) 621
BpmI CTGGAG 2 cut(s) 90, 378
BpuEI CTTGAG 1 cut(s) 227
BpuMI CCSGG 1 cut(s) 623
BsaI GGTCTC 1 cut(s) 187
BsaJI CCNNGG 2 cut(s) 150, 585
BsaXI ACNNNNNCTCC 2 cut(s) 480, 510
Bsc4I CCNNNNNNNGG 4 cut(s) 220, 628, 682, 683
Bse1I ACTGG 1 cut(s) 504
Bse3DI GCAATG 1 cut(s) 55
BseBI CCWGG 2 cut(s) 152, 446
BseDI CCNNGG 2 cut(s) 150, 585
BseGI GGATG 2 cut(s) 237, 709
BseLI CCNNNNNNNGG 4 cut(s) 220, 628, 682, 683
BseMI GCAATG 1 cut(s) 55
BseMII CTCAG 1 cut(s) 353
BseNI ACTGG 1 cut(s) 504
BseRI GAGGAG 1 cut(s) 662
Bsh1236I CGCG 4 cut(s) 311, 313, 373, 454
BshFI GGCC 1 cut(s) 282
BsiHKAI GWGCWC 2 cut(s) 268, 380
BsiSI CCGG 1 cut(s) 622
BslFI GGGAC 2 cut(s) 368, 675
BslI CCNNNNNNNGG 4 cut(s) 220, 628, 682, 683
BsmAI GTCTC 2 cut(s) 187, 650
BsmBI CGTCTC 1 cut(s) 650
BsmFI GGGAC 2 cut(s) 368, 675
BsnI GGCC 1 cut(s) 282
Bso31I GGTCTC 1 cut(s) 187
Bsp1286I GDGCHC 2 cut(s) 268, 380
Bsp143I GATC 4 cut(s) 86, 303, 381, 671
BspACI CCGC 6 cut(s) 293, 373, 521, 548, 563, 683
BspANI GGCC 1 cut(s) 282
BspCNI CTCAG 1 cut(s) 352
BspFNI CGCG 4 cut(s) 311, 313, 373, 454
BspLI GGNNCC 2 cut(s) 663, 673
BspPI GGATC 3 cut(s) 94, 666, 679
BspTNI GGTCTC 1 cut(s) 187
BsrDI GCAATG 1 cut(s) 55
BsrI ACTGG 1 cut(s) 504
BssECI CCNNGG 2 cut(s) 150, 585
BssMI GATC 4 cut(s) 86, 303, 381, 671
BssSI CACGAG 1 cut(s) 299
Bst2BI CACGAG 1 cut(s) 299
Bst2UI CCWGG 2 cut(s) 152, 446
Bst4CI ACNGT 1 cut(s) 278
Bst6I CTCTTC 1 cut(s) 621
BstC8I GCNNGC 1 cut(s) 293
BstDEI CTNAG 2 cut(s) 99, 339
BstDSI CCRYGG 1 cut(s) 585
BstF5I GGATG 2 cut(s) 237, 709
BstFNI CGCG 4 cut(s) 311, 313, 373, 454
BstHHI GCGC 3 cut(s) 313, 454, 469
BstKTI GATC 4 cut(s) 89, 306, 384, 674
BstMAI GTCTC 2 cut(s) 187, 650
BstMBI GATC 4 cut(s) 86, 303, 381, 671
BstMWI GCNNNNNNNGC 2 cut(s) 184, 288
BstNI CCWGG 2 cut(s) 152, 446
BstSCI CCNGG 3 cut(s) 150, 444, 621
BstUI CGCG 4 cut(s) 311, 313, 373, 454
BstX2I RGATCY 1 cut(s) 671
BstYI RGATCY 1 cut(s) 671
BsuI GTATCC 1 cut(s) 124
BsuRI GGCC 1 cut(s) 282
BtgI CCRYGG 1 cut(s) 585
BtsCI GGATG 2 cut(s) 237, 709
BtsIMutI CAGTG 1 cut(s) 274
Cac8I GCNNGC 1 cut(s) 293
CfoI GCGC 3 cut(s) 313, 454, 469
Cfr13I GGNCC 4 cut(s) 8, 223, 254, 662
CseI GACGC 1 cut(s) 317
Csp6I GTAC 2 cut(s) 404, 497
CviAII CATG 2 cut(s) 251, 484
CviQI GTAC 2 cut(s) 404, 497
DdeI CTNAG 2 cut(s) 99, 339
DpnI GATC 4 cut(s) 88, 305, 383, 673
DpnII GATC 4 cut(s) 86, 303, 381, 671
EaeI YGGCCR 1 cut(s) 280
Eam1104I CTCTTC 1 cut(s) 621
EarI CTCTTC 1 cut(s) 621
EciI GGCGGA 2 cut(s) 536, 563
Ecl136II GAGCTC 2 cut(s) 266, 378
Eco24I GRGCYC 2 cut(s) 268, 380
Eco31I GGTCTC 1 cut(s) 187
Eco32I GATATC 2 cut(s) 146, 163
Eco47I GGWCC 4 cut(s) 8, 223, 254, 662
Eco53kI GAGCTC 2 cut(s) 266, 378
Eco57I CTGAAG 1 cut(s) 203
EcoICRI GAGCTC 2 cut(s) 266, 378
EcoRII CCWGG 2 cut(s) 150, 444
EcoRV GATATC 2 cut(s) 146, 163
EcoT38I GRGCYC 2 cut(s) 268, 380
Esp3I CGTCTC 1 cut(s) 650
FaeI CATG 2 cut(s) 254, 487
FaiI YATR 4 cut(s) 176, 252, 362, 485
FaqI GGGAC 2 cut(s) 368, 675
FatI CATG 2 cut(s) 250, 483
FauI CCCGC 2 cut(s) 300, 676
FblI GTMKAC 1 cut(s) 480
FokI GGATG 1 cut(s) 224
FriOI GRGCYC 2 cut(s) 268, 380
FspBI CTAG 1 cut(s) 288
GlaI GCGC 3 cut(s) 312, 453, 468
GsuI CTGGAG 2 cut(s) 90, 378
HaeIII GGCC 1 cut(s) 282
HapII CCGG 1 cut(s) 622
HgaI GACGC 1 cut(s) 317
HhaI GCGC 3 cut(s) 313, 454, 469
Hin1II CATG 2 cut(s) 254, 487
Hin6I GCGC 3 cut(s) 311, 452, 467
HinP1I GCGC 3 cut(s) 311, 452, 467
HincII GTYRAC 3 cut(s) 481, 568, 619
HindII GTYRAC 3 cut(s) 481, 568, 619
HindIII AAGCTT 1 cut(s) 92
HinfI GANTC 1 cut(s) 477
HpaII CCGG 1 cut(s) 622
HphI GGTGA 1 cut(s) 251
Hpy166II GTNNAC 4 cut(s) 8, 481, 568, 619
Hpy188I TCNGA 1 cut(s) 183
Hpy188III TCNNGA 3 cut(s) 69, 244, 395
Hpy8I GTNNAC 4 cut(s) 8, 481, 568, 619
Hpy99I CGWCG 1 cut(s) 311
HpyAV CCTTC 2 cut(s) 49, 518
HpyCH4III ACNGT 1 cut(s) 278
HpyF10VI GCNNNNNNNGC 2 cut(s) 184, 288
HpyF3I CTNAG 2 cut(s) 99, 339
Hsp92II CATG 2 cut(s) 254, 487
HspAI GCGC 3 cut(s) 311, 452, 467
Kzo9I GATC 4 cut(s) 86, 303, 381, 671
LmnI GCTCC 3 cut(s) 216, 375, 397
LweI GCATC 1 cut(s) 621
MaeI CTAG 1 cut(s) 288
MaeIII GTNAC 2 cut(s) 367, 455
MalI GATC 4 cut(s) 88, 305, 383, 673
MboI GATC 4 cut(s) 86, 303, 381, 671
MboII GAAGA 4 cut(s) 376, 431, 518, 638
MflI RGATCY 1 cut(s) 671
MhlI GDGCHC 2 cut(s) 268, 380
MlsI TGGCCA 1 cut(s) 282
MluCI AATT 2 cut(s) 103, 540
MluNI TGGCCA 1 cut(s) 282
MlyI GAGTC 1 cut(s) 486
Mox20I TGGCCA 1 cut(s) 282
MscI TGGCCA 1 cut(s) 282
MseI TTAA 1 cut(s) 18
Msp20I TGGCCA 1 cut(s) 282
MspI CCGG 1 cut(s) 622
MspR9I CCNGG 3 cut(s) 152, 446, 623
MvaI CCWGG 2 cut(s) 152, 446
MvnI CGCG 4 cut(s) 311, 313, 373, 454
MwoI GCNNNNNNNGC 2 cut(s) 184, 288
NciI CCSGG 1 cut(s) 623
NdeII GATC 4 cut(s) 86, 303, 381, 671
NlaIII CATG 2 cut(s) 254, 487
NlaIV GGNNCC 2 cut(s) 663, 673
NmeAIII GCCGAG 1 cut(s) 555
NmuCI GTSAC 2 cut(s) 367, 455
PcsI WCGNNNNNNNCGW 3 cut(s) 377, 576, 582
PleI GAGTC 1 cut(s) 485
PpsI GAGTC 1 cut(s) 485
Psp124BI GAGCTC 2 cut(s) 268, 380
Psp6I CCWGG 2 cut(s) 150, 444
PspGI CCWGG 2 cut(s) 150, 444
PspN4I GGNNCC 2 cut(s) 663, 673
PspPI GGNCC 4 cut(s) 8, 223, 254, 662
PsuI RGATCY 1 cut(s) 671
RsaI GTAC 2 cut(s) 405, 498
RsaNI GTAC 2 cut(s) 404, 497
SacI GAGCTC 2 cut(s) 268, 380
SalI GTCGAC 1 cut(s) 479
SaqAI TTAA 1 cut(s) 18
Sau3AI GATC 4 cut(s) 86, 303, 381, 671
Sau96I GGNCC 4 cut(s) 8, 223, 254, 662
ScaI AGTACT 1 cut(s) 498
SchI GAGTC 1 cut(s) 486
ScrFI CCNGG 3 cut(s) 152, 446, 623
SduI GDGCHC 2 cut(s) 268, 380
SfaNI GCATC 1 cut(s) 621
SinI GGWCC 4 cut(s) 8, 223, 254, 662
SmlI CTYRAG 1 cut(s) 242
SmoI CTYRAG 1 cut(s) 242
Sse9I AATT 2 cut(s) 103, 540
SsiI CCGC 6 cut(s) 293, 373, 521, 548, 563, 683
SspMI CTAG 1 cut(s) 288
SstI GAGCTC 2 cut(s) 268, 380
StyD4I CCNGG 3 cut(s) 150, 444, 621
TaaI ACNGT 1 cut(s) 278
TaqI TCGA 6 cut(s) 306, 380, 414, 426, 480, 645
TasI AATT 2 cut(s) 103, 540
TatI WGTACW 1 cut(s) 496
Tru1I TTAA 1 cut(s) 18
Tru9I TTAA 1 cut(s) 18
TscAI CASTG 1 cut(s) 281
TseFI GTSAC 2 cut(s) 367, 455
Tsp45I GTSAC 2 cut(s) 367, 455
TspDTI ATGAA 2 cut(s) 432, 525
TspRI CASTG 1 cut(s) 281
VpaK11BI GGWCC 4 cut(s) 8, 223, 254, 662
XapI RAATTY 1 cut(s) 103
XmiI GTMKAC 1 cut(s) 480
XspI CTAG 1 cut(s) 288
ZrmI AGTACT 1 cut(s) 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.