Rh2DG573900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0001849
Physical Location & Seq
Reverse (-)
80337581 .. 80338156
576 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG573900.1

Sequence Viewer

Length: 576 bp
ATGCGTGAGCCTCAAGACTTCTACACATCCATGTCTGAGCAACGAGAGATGAAAATCATAAAGCCAGGTGTCTCCGTCGTGGTAGTTATCGTCGATGAAGAAGTCGAGGTGGAGCTGACGCGCCTTGAGGCGCGTGACGGCGCAGCGGATCCAAGAGTCGACGTGGATGTGGTAGCCTTGGTGGAAGATGAAGGAGAGGCGGAAGGTTTGGATGGGGGAGTCGGGGCGGAGAATGAGAGCGCGATCGATGAAGTCGGCGAAGAATTGGGCCTGGTCCTGGGTCGGGTTGAGATCGGATTGGGGCGCGGGAGGGAAGAGGTGGTAGGAGAAGTTCAAGGAGGGAACGAGGGACCAGAGGGGTCGCCATCTGCGGGAGATGAGAGAGGTGTTGACGGCGTCGAGGGTGGGGAGGAGGGAGAGGATGTGGAGGAGGATCTCGTCGGGGAGGTCGTTGAGACTCCGGTTAGGGCCGCGGAGGCGTTTGGCGTGGAGGAGGAGGACTTGAGTGCTCGTCTCTGCTGCCATGTGTCTGGATTTTCCGGGAGACAGAGTCTTTGTGTAGGAGAAGACCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

191

Amino Acids

20.11

Weight (kDa)

4.05

Isoelectric Point (pI)

35.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 159
AccII CGCG 5 cut(s) 121, 133, 242, 306, 473
AciI CCGC 7 cut(s) 146, 200, 227, 306, 371, 471, 473
AclWI GGATC 3 cut(s) 143, 156, 441
AcyI GRCGYC 1 cut(s) 396
AfiI CCNNNNNNNGG 3 cut(s) 277, 283, 371
AgsI TTSAA 1 cut(s) 335
AjiI CACGTC 1 cut(s) 163
AjnI CCWGG 3 cut(s) 64, 270, 276
AluBI AGCT 1 cut(s) 115
AluI AGCT 1 cut(s) 115
Alw21I GWGCWC 1 cut(s) 511
Alw26I GTCTC 4 cut(s) 76, 449, 518, 538
AlwI GGATC 3 cut(s) 143, 156, 441
AoxI GGCC 2 cut(s) 268, 468
ApeKI GCWGC 2 cut(s) 143, 519
ArsI GACNNNNNNTTYG 2 cut(s) 537, 569
AspLEI GCGC 5 cut(s) 123, 133, 143, 242, 306
AspS9I GGNCC 4 cut(s) 268, 274, 350, 468
AsuC2I CCSGG 1 cut(s) 541
AvaII GGWCC 2 cut(s) 274, 350
BamHI GGATCC 1 cut(s) 148
Bbv12I GWGCWC 1 cut(s) 511
BbvI GCAGC 2 cut(s) 155, 506
BccI CCATC 2 cut(s) 206, 373
BceAI ACGGC 2 cut(s) 154, 409
BciT130I CCWGG 3 cut(s) 66, 272, 278
BcnI CCSGG 1 cut(s) 541
BcoDI GTCTC 4 cut(s) 76, 449, 518, 538
BglI GCCNNNNNGGC 1 cut(s) 476
BisI GCNGC 3 cut(s) 144, 471, 520
BlsI GCNGC 3 cut(s) 145, 472, 521
Bme1390I CCNGG 4 cut(s) 66, 272, 278, 541
Bme18I GGWCC 2 cut(s) 274, 350
BmgBI CACGTC 1 cut(s) 163
BmgT120I GGNCC 4 cut(s) 268, 274, 350, 468
BmiI GGNNCC 2 cut(s) 150, 351
BmrFI CCNGG 4 cut(s) 66, 272, 278, 541
BpuEI CTTGAG 2 cut(s) 146, 523
BpuMI CCSGG 1 cut(s) 541
Bsa29I ATCGAT 1 cut(s) 246
BsaBI GATNNNNATC 1 cut(s) 53
BsaHI GRCGYC 1 cut(s) 396
BsaJI CCNNGG 3 cut(s) 177, 277, 471
BsaWI WCCGGW 1 cut(s) 460
BsaXI ACNNNNNCTCC 4 cut(s) 408, 438, 535, 565
Bsc4I CCNNNNNNNGG 3 cut(s) 277, 283, 371
Bse8I GATNNNNATC 1 cut(s) 53
BseBI CCWGG 3 cut(s) 66, 272, 278
BseCI ATCGAT 1 cut(s) 246
BseDI CCNNGG 3 cut(s) 177, 277, 471
BseGI GGATG 4 cut(s) 26, 172, 217, 427
BseJI GATNNNNATC 1 cut(s) 53
BseLI CCNNNNNNNGG 3 cut(s) 277, 283, 371
BseMII CTCAG 1 cut(s) 27
BseRI GAGGAG 4 cut(s) 425, 443, 506, 509
BseXI GCAGC 2 cut(s) 155, 506
Bsh1236I CGCG 5 cut(s) 121, 133, 242, 306, 473
Bsh1285I CGRYCG 1 cut(s) 246
BshFI GGCC 2 cut(s) 270, 470
BshVI ATCGAT 1 cut(s) 246
BsiEI CGRYCG 1 cut(s) 246
BsiHKAI GWGCWC 1 cut(s) 511
BsiSI CCGG 2 cut(s) 461, 540
BslFI GGGAC 1 cut(s) 363
BslI CCNNNNNNNGG 3 cut(s) 277, 283, 371
BsmAI GTCTC 4 cut(s) 76, 449, 518, 538
BsmBI CGTCTC 1 cut(s) 518
BsmFI GGGAC 1 cut(s) 363
BsnI GGCC 2 cut(s) 270, 470
Bsp1286I GDGCHC 1 cut(s) 511
Bsp143I GATC 4 cut(s) 148, 243, 291, 433
BspACI CCGC 7 cut(s) 146, 200, 227, 306, 371, 471, 473
BspANI GGCC 2 cut(s) 270, 470
BspCNI CTCAG 1 cut(s) 28
BspDI ATCGAT 1 cut(s) 246
BspFNI CGCG 5 cut(s) 121, 133, 242, 306, 473
BspLI GGNNCC 2 cut(s) 150, 351
BspPI GGATC 3 cut(s) 143, 156, 441
BssECI CCNNGG 3 cut(s) 177, 277, 471
BssMI GATC 4 cut(s) 148, 243, 291, 433
BssNI GRCGYC 1 cut(s) 396
BssT1I CCWWGG 1 cut(s) 177
Bst2UI CCWGG 3 cut(s) 66, 272, 278
Bst6I CTCTTC 1 cut(s) 309
BstACI GRCGYC 1 cut(s) 396
BstDEI CTNAG 1 cut(s) 36
BstDSI CCRYGG 1 cut(s) 471
BstF5I GGATG 4 cut(s) 26, 172, 217, 427
BstFNI CGCG 5 cut(s) 121, 133, 242, 306, 473
BstHHI GCGC 5 cut(s) 123, 133, 143, 242, 306
BstKTI GATC 4 cut(s) 151, 246, 294, 436
BstMAI GTCTC 4 cut(s) 76, 449, 518, 538
BstMBI GATC 4 cut(s) 148, 243, 291, 433
BstMCI CGRYCG 1 cut(s) 246
BstMWI GCNNNNNNNGC 1 cut(s) 476
BstNI CCWGG 3 cut(s) 66, 272, 278
BstSCI CCNGG 4 cut(s) 64, 270, 276, 539
BstUI CGCG 5 cut(s) 121, 133, 242, 306, 473
BstV1I GCAGC 2 cut(s) 155, 506
BstX2I RGATCY 2 cut(s) 148, 433
BstXI CCANNNNNNTGG 1 cut(s) 530
BstYI RGATCY 2 cut(s) 148, 433
Bsu15I ATCGAT 1 cut(s) 246
BsuRI GGCC 2 cut(s) 270, 470
BsuTUI ATCGAT 1 cut(s) 246
BtgI CCRYGG 1 cut(s) 471
BtrI CACGTC 1 cut(s) 163
BtsCI GGATG 4 cut(s) 26, 172, 217, 427
CfoI GCGC 5 cut(s) 123, 133, 143, 242, 306
Cfr13I GGNCC 4 cut(s) 268, 274, 350, 468
Cfr42I CCGCGG 1 cut(s) 474
ClaI ATCGAT 1 cut(s) 246
CseI GACGC 2 cut(s) 127, 385
CviAII CATG 2 cut(s) 31, 524
CviJI RGCY 6 cut(s) 10, 64, 115, 176, 270, 470
CviKI_1 RGCY 6 cut(s) 10, 64, 115, 176, 270, 470
DdeI CTNAG 1 cut(s) 36
DpnI GATC 4 cut(s) 150, 245, 293, 435
DpnII GATC 4 cut(s) 148, 243, 291, 433
Eam1104I CTCTTC 1 cut(s) 309
EarI CTCTTC 1 cut(s) 309
EciI GGCGGA 2 cut(s) 215, 242
Eco130I CCWWGG 1 cut(s) 177
Eco47I GGWCC 2 cut(s) 274, 350
EcoRII CCWGG 3 cut(s) 64, 270, 276
EcoT14I CCWWGG 1 cut(s) 177
ErhI CCWWGG 1 cut(s) 177
Esp3I CGTCTC 1 cut(s) 518
FaeI CATG 2 cut(s) 34, 527
FaiI YATR 4 cut(s) 32, 59, 525, 574
FaqI GGGAC 1 cut(s) 363
FatI CATG 2 cut(s) 30, 523
FauI CCCGC 2 cut(s) 299, 364
FblI GTMKAC 1 cut(s) 159
Fnu4HI GCNGC 3 cut(s) 144, 471, 520
FokI GGATG 4 cut(s) 13, 179, 224, 434
Fsp4HI GCNGC 3 cut(s) 144, 471, 520
GlaI GCGC 5 cut(s) 122, 132, 142, 241, 305
GluI GCNGC 3 cut(s) 144, 471, 520
HaeIII GGCC 2 cut(s) 270, 470
HapII CCGG 2 cut(s) 461, 540
HgaI GACGC 2 cut(s) 127, 385
HhaI GCGC 5 cut(s) 123, 133, 143, 242, 306
Hin1I GRCGYC 1 cut(s) 396
Hin1II CATG 2 cut(s) 34, 527
Hin6I GCGC 5 cut(s) 121, 131, 141, 240, 304
HinP1I GCGC 5 cut(s) 121, 131, 141, 240, 304
HincII GTYRAC 2 cut(s) 160, 391
HindII GTYRAC 2 cut(s) 160, 391
HinfI GANTC 4 cut(s) 156, 219, 457, 550
HpaII CCGG 2 cut(s) 461, 540
Hpy166II GTNNAC 2 cut(s) 160, 391
Hpy188I TCNGA 2 cut(s) 37, 296
Hpy188III TCNNGA 2 cut(s) 14, 531
Hpy8I GTNNAC 2 cut(s) 160, 391
Hpy99I CGWCG 5 cut(s) 80, 95, 164, 401, 443
HpyAV CCTTC 2 cut(s) 185, 197
HpyCH4IV ACGT 1 cut(s) 162
HpyF10VI GCNNNNNNNGC 1 cut(s) 476
HpyF3I CTNAG 1 cut(s) 36
HpySE526I ACGT 1 cut(s) 162
Hsp92I GRCGYC 1 cut(s) 396
Hsp92II CATG 2 cut(s) 34, 527
HspAI GCGC 5 cut(s) 121, 131, 141, 240, 304
KspI CCGCGG 1 cut(s) 474
Kzo9I GATC 4 cut(s) 148, 243, 291, 433
LmnI GCTCC 1 cut(s) 112
Lsp1109I GCAGC 2 cut(s) 155, 506
MaeII ACGT 1 cut(s) 162
MaeIII GTNAC 1 cut(s) 134
MalI GATC 4 cut(s) 150, 245, 293, 435
MboI GATC 4 cut(s) 148, 243, 291, 433
MboII GAAGA 4 cut(s) 110, 197, 272, 326
MflI RGATCY 2 cut(s) 148, 433
MhlI GDGCHC 1 cut(s) 511
MluCI AATT 1 cut(s) 263
MlyI GAGTC 4 cut(s) 165, 228, 451, 559
MslI CAYNNNNRTG 1 cut(s) 29
MspA1I CMGCKG 2 cut(s) 146, 473
MspI CCGG 2 cut(s) 461, 540
MspR9I CCNGG 4 cut(s) 66, 272, 278, 541
MvaI CCWGG 3 cut(s) 66, 272, 278
MvnI CGCG 5 cut(s) 121, 133, 242, 306, 473
MwoI GCNNNNNNNGC 1 cut(s) 476
NciI CCSGG 1 cut(s) 541
NdeII GATC 4 cut(s) 148, 243, 291, 433
NlaIII CATG 2 cut(s) 34, 527
NlaIV GGNNCC 2 cut(s) 150, 351
NmuCI GTSAC 1 cut(s) 134
PcsI WCGNNNNNNNCGW 1 cut(s) 447
PflFI GACNNNGTC 2 cut(s) 395, 549
PfoI TCCNGGA 1 cut(s) 539
PkrI GCNGC 3 cut(s) 145, 472, 521
Ple19I CGATCG 1 cut(s) 246
PleI GAGTC 4 cut(s) 164, 227, 451, 558
PpsI GAGTC 4 cut(s) 164, 227, 451, 558
Psp6I CCWGG 3 cut(s) 64, 270, 276
PspGI CCWGG 3 cut(s) 64, 270, 276
PspN4I GGNNCC 2 cut(s) 150, 351
PspPI GGNCC 4 cut(s) 268, 274, 350, 468
PsrI GAACNNNNNNTAC 2 cut(s) 315, 347
PsuI RGATCY 2 cut(s) 148, 433
PsyI GACNNNGTC 2 cut(s) 395, 549
PvuI CGATCG 1 cut(s) 246
RseI CAYNNNNRTG 1 cut(s) 29
SacII CCGCGG 1 cut(s) 474
SalI GTCGAC 1 cut(s) 158
SatI GCNGC 3 cut(s) 144, 471, 520
Sau3AI GATC 4 cut(s) 148, 243, 291, 433
Sau96I GGNCC 4 cut(s) 268, 274, 350, 468
SchI GAGTC 4 cut(s) 165, 228, 451, 559
ScrFI CCNGG 4 cut(s) 66, 272, 278, 541
SduI GDGCHC 1 cut(s) 511
SetI ASST 9 cut(s) 70, 111, 117, 165, 208, 321, 388, 450, 573
Sfr303I CCGCGG 1 cut(s) 474
SgrBI CCGCGG 1 cut(s) 474
SinI GGWCC 2 cut(s) 274, 350
SmiMI CAYNNNNRTG 1 cut(s) 29
SmlI CTYRAG 3 cut(s) 12, 125, 502
SmoI CTYRAG 3 cut(s) 12, 125, 502
Sse9I AATT 1 cut(s) 263
SsiI CCGC 7 cut(s) 146, 200, 227, 306, 371, 471, 473
StyD4I CCNGG 4 cut(s) 64, 270, 276, 539
StyI CCWWGG 1 cut(s) 177
TaiI ACGT 1 cut(s) 165
TaqI TCGA 5 cut(s) 93, 105, 159, 246, 399
TasI AATT 1 cut(s) 263
TauI GCSGC 1 cut(s) 473
TseFI GTSAC 1 cut(s) 134
TseI GCWGC 2 cut(s) 143, 519
Tsp45I GTSAC 1 cut(s) 134
TspDTI ATGAA 4 cut(s) 65, 111, 204, 264
TspGWI ACGGA 1 cut(s) 64
Tth111I GACNNNGTC 2 cut(s) 395, 549
VpaK11BI GGWCC 2 cut(s) 274, 350
XmiI GTMKAC 1 cut(s) 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.