Rh2BG116100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0001849
Physical Location & Seq
Forward (+)
9836538 .. 9836921
384 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG116100.1

Sequence Viewer

Length: 384 bp
ATGGTGAGGATGCTTCGGGTTCGAGTGCTATGTGTTGCTCCGAACACAGGGAAGAGTGTTGGGTTCTGTGTTGCTTCATTGAGCTCTGTCTTCCAAGGTTGCCACGAACGACACCGCCGCCACCAACCCTGCAGCCGTACCGCCACCATTGCTACTACCCCGAGTTCACGGTCACAGCGCGGTCGCAATGGGAGTTCATGGGTCGAATCCAAGCTGGGTACTGCTGATGGGTTATCCTGGTGGTCCGATTTGGAAGAGAGCGCGATCGATGAAGTCAGCGAAGAATTCAGCCTGGGCCTGTGTCGGGTTGAGATCGGATTTGGGGGCCGGAGGAAAGAGGTGGTAGGAGAAGTTCAAGGAGGGAACGAGGGACCAGAGGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

127

Amino Acids

13.78

Weight (kDa)

7.68

Isoelectric Point (pI)

47.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 180, 263
AciI CCGC 4 cut(s) 115, 118, 141, 180
AcsI RAATTY 1 cut(s) 284
AfaI GTAC 2 cut(s) 139, 220
AfiI CCNNNNNNNGG 2 cut(s) 47, 304
AgsI TTSAA 1 cut(s) 356
AjnI CCWGG 2 cut(s) 236, 291
AluBI AGCT 2 cut(s) 84, 214
AluI AGCT 2 cut(s) 84, 214
Alw21I GWGCWC 1 cut(s) 86
Ama87I CYCGRG 1 cut(s) 160
AoxI GGCC 2 cut(s) 295, 325
ApeKI GCWGC 1 cut(s) 132
ApoI RAATTY 1 cut(s) 284
AspLEI GCGC 2 cut(s) 180, 263
AspS9I GGNCC 4 cut(s) 243, 295, 325, 371
AsuHPI GGTGA 1 cut(s) 16
AvaI CYCGRG 1 cut(s) 160
AvaII GGWCC 2 cut(s) 243, 371
BanII GRGCYC 1 cut(s) 86
BbsI GAAGAC 1 cut(s) 82
Bbv12I GWGCWC 1 cut(s) 86
BbvI GCAGC 1 cut(s) 144
BccI CCATC 1 cut(s) 221
BceAI ACGGC 1 cut(s) 120
BciT130I CCWGG 2 cut(s) 238, 293
BfmI CTRYAG 1 cut(s) 130
BisI GCNGC 2 cut(s) 118, 133
BlsI GCNGC 2 cut(s) 119, 134
Bme1390I CCNGG 2 cut(s) 238, 293
Bme18I GGWCC 2 cut(s) 243, 371
BmeT110I CYCGRG 1 cut(s) 160
BmgT120I GGNCC 4 cut(s) 243, 295, 325, 371
BmiI GGNNCC 2 cut(s) 326, 372
BmrFI CCNGG 2 cut(s) 238, 293
BpiI GAAGAC 1 cut(s) 82
Bsa29I ATCGAT 1 cut(s) 267
BsaJI CCNNGG 2 cut(s) 94, 292
Bsc4I CCNNNNNNNGG 2 cut(s) 47, 304
Bse3DI GCAATG 2 cut(s) 147, 193
BseBI CCWGG 2 cut(s) 238, 293
BseCI ATCGAT 1 cut(s) 267
BseDI CCNNGG 2 cut(s) 94, 292
BseGI GGATG 1 cut(s) 15
BseLI CCNNNNNNNGG 2 cut(s) 47, 304
BseMI GCAATG 2 cut(s) 147, 193
BseXI GCAGC 1 cut(s) 144
BseYI CCCAGC 1 cut(s) 214
Bsh1236I CGCG 2 cut(s) 180, 263
Bsh1285I CGRYCG 2 cut(s) 184, 267
BshFI GGCC 2 cut(s) 297, 327
BshVI ATCGAT 1 cut(s) 267
BsiEI CGRYCG 2 cut(s) 184, 267
BsiHKAI GWGCWC 1 cut(s) 86
BsiHKCI CYCGRG 1 cut(s) 160
BsiSI CCGG 1 cut(s) 328
BslI CCNNNNNNNGG 2 cut(s) 47, 304
BsnI GGCC 2 cut(s) 297, 327
BsoBI CYCGRG 1 cut(s) 160
Bsp1286I GDGCHC 1 cut(s) 86
Bsp143I GATC 2 cut(s) 264, 312
BspACI CCGC 4 cut(s) 115, 118, 141, 180
BspANI GGCC 2 cut(s) 297, 327
BspDI ATCGAT 1 cut(s) 267
BspFNI CGCG 2 cut(s) 180, 263
BspLI GGNNCC 2 cut(s) 326, 372
BspMAI CTGCAG 1 cut(s) 134
BsrDI GCAATG 2 cut(s) 147, 193
BssECI CCNNGG 2 cut(s) 94, 292
BssMI GATC 2 cut(s) 264, 312
BssT1I CCWWGG 1 cut(s) 94
Bst2UI CCWGG 2 cut(s) 238, 293
Bst4CI ACNGT 1 cut(s) 171
Bst6I CTCTTC 2 cut(s) 47, 249
BstF5I GGATG 1 cut(s) 15
BstFNI CGCG 2 cut(s) 180, 263
BstHHI GCGC 2 cut(s) 180, 263
BstKTI GATC 2 cut(s) 267, 315
BstMBI GATC 2 cut(s) 264, 312
BstMCI CGRYCG 2 cut(s) 184, 267
BstMWI GCNNNNNNNGC 1 cut(s) 149
BstNI CCWGG 2 cut(s) 238, 293
BstSCI CCNGG 2 cut(s) 236, 291
BstSFI CTRYAG 1 cut(s) 130
BstUI CGCG 2 cut(s) 180, 263
BstV1I GCAGC 1 cut(s) 144
BstV2I GAAGAC 1 cut(s) 82
Bsu15I ATCGAT 1 cut(s) 267
BsuRI GGCC 2 cut(s) 297, 327
BsuTUI ATCGAT 1 cut(s) 267
BtsCI GGATG 1 cut(s) 15
CfoI GCGC 2 cut(s) 180, 263
Cfr13I GGNCC 4 cut(s) 243, 295, 325, 371
ClaI ATCGAT 1 cut(s) 267
Csp6I GTAC 2 cut(s) 138, 219
CviAII CATG 1 cut(s) 198
CviJI RGCY 6 cut(s) 84, 135, 214, 291, 297, 327
CviKI_1 RGCY 6 cut(s) 84, 135, 214, 291, 297, 327
CviQI GTAC 2 cut(s) 138, 219
DpnI GATC 2 cut(s) 266, 314
DpnII GATC 2 cut(s) 264, 312
Eam1104I CTCTTC 2 cut(s) 47, 249
EarI CTCTTC 2 cut(s) 47, 249
Ecl136II GAGCTC 1 cut(s) 84
Eco130I CCWWGG 1 cut(s) 94
Eco24I GRGCYC 1 cut(s) 86
Eco47I GGWCC 2 cut(s) 243, 371
Eco53kI GAGCTC 1 cut(s) 84
Eco88I CYCGRG 1 cut(s) 160
EcoICRI GAGCTC 1 cut(s) 84
EcoRI GAATTC 1 cut(s) 284
EcoRII CCWGG 2 cut(s) 236, 291
EcoT14I CCWWGG 1 cut(s) 94
EcoT38I GRGCYC 1 cut(s) 86
ErhI CCWWGG 1 cut(s) 94
FaeI CATG 1 cut(s) 201
FaiI YATR 2 cut(s) 31, 199
FatI CATG 1 cut(s) 197
Fnu4HI GCNGC 2 cut(s) 118, 133
FokI GGATG 1 cut(s) 22
FriOI GRGCYC 1 cut(s) 86
Fsp4HI GCNGC 2 cut(s) 118, 133
GlaI GCGC 2 cut(s) 179, 262
GluI GCNGC 2 cut(s) 118, 133
GsaI CCCAGC 1 cut(s) 218
HaeIII GGCC 2 cut(s) 297, 327
HapII CCGG 1 cut(s) 328
HhaI GCGC 2 cut(s) 180, 263
Hin1II CATG 1 cut(s) 201
Hin6I GCGC 2 cut(s) 178, 261
HinP1I GCGC 2 cut(s) 178, 261
HinfI GANTC 1 cut(s) 206
HpaII CCGG 1 cut(s) 328
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 1 cut(s) 167
Hpy188I TCNGA 3 cut(s) 42, 247, 317
Hpy8I GTNNAC 1 cut(s) 167
HpyCH4III ACNGT 1 cut(s) 171
HpyCH4V TGCA 1 cut(s) 132
HpyF10VI GCNNNNNNNGC 1 cut(s) 149
Hsp92II CATG 1 cut(s) 201
HspAI GCGC 2 cut(s) 178, 261
Kzo9I GATC 2 cut(s) 264, 312
LmnI GCTCC 1 cut(s) 43
LpnPI CCDG 9 cut(s) 33, 142, 200, 223, 250, 278, 305, 311, 341
Lsp1109I GCAGC 1 cut(s) 144
MaeIII GTNAC 1 cut(s) 171
MalI GATC 2 cut(s) 266, 314
MboI GATC 2 cut(s) 264, 312
MboII GAAGA 4 cut(s) 64, 82, 266, 293
MhlI GDGCHC 1 cut(s) 86
MluCI AATT 1 cut(s) 284
MnlI CCTC 5 cut(s) 324, 331, 353, 361, 370
MspI CCGG 1 cut(s) 328
MspR9I CCNGG 2 cut(s) 238, 293
MvaI CCWGG 2 cut(s) 238, 293
MvnI CGCG 2 cut(s) 180, 263
MwoI GCNNNNNNNGC 1 cut(s) 149
NdeII GATC 2 cut(s) 264, 312
NlaIII CATG 1 cut(s) 201
NlaIV GGNNCC 2 cut(s) 326, 372
NmuCI GTSAC 1 cut(s) 171
PfeI GAWTC 1 cut(s) 206
PkrI GCNGC 2 cut(s) 119, 134
Ple19I CGATCG 1 cut(s) 267
Psp124BI GAGCTC 1 cut(s) 86
Psp6I CCWGG 2 cut(s) 236, 291
PspFI CCCAGC 1 cut(s) 214
PspGI CCWGG 2 cut(s) 236, 291
PspN4I GGNNCC 2 cut(s) 326, 372
PspPI GGNCC 4 cut(s) 243, 295, 325, 371
PsrI GAACNNNNNNTAC 2 cut(s) 336, 368
PstI CTGCAG 1 cut(s) 134
PvuI CGATCG 1 cut(s) 267
RsaI GTAC 2 cut(s) 139, 220
RsaNI GTAC 2 cut(s) 138, 219
SacI GAGCTC 1 cut(s) 86
SatI GCNGC 2 cut(s) 118, 133
Sau3AI GATC 2 cut(s) 264, 312
Sau96I GGNCC 4 cut(s) 243, 295, 325, 371
ScrFI CCNGG 2 cut(s) 238, 293
SduI GDGCHC 1 cut(s) 86
SetI ASST 4 cut(s) 86, 100, 216, 342
SfcI CTRYAG 1 cut(s) 130
SinI GGWCC 2 cut(s) 243, 371
Sse9I AATT 1 cut(s) 284
SsiI CCGC 4 cut(s) 115, 118, 141, 180
SstI GAGCTC 1 cut(s) 86
StyD4I CCNGG 2 cut(s) 236, 291
StyI CCWWGG 1 cut(s) 94
TaaI ACNGT 1 cut(s) 171
TaqI TCGA 3 cut(s) 22, 204, 267
TasI AATT 1 cut(s) 284
TauI GCSGC 1 cut(s) 120
TfiI GAWTC 1 cut(s) 206
TseFI GTSAC 1 cut(s) 171
TseI GCWGC 1 cut(s) 132
Tsp45I GTSAC 1 cut(s) 171
TspDTI ATGAA 3 cut(s) 66, 186, 285
VpaK11BI GGWCC 2 cut(s) 243, 371
XapI RAATTY 1 cut(s) 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.