RLG00000006094

Jasmonate O-methyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0001051
Physical Location & Seq
Forward (+)
4330825 .. 4333878
3054 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006094

Sequence Viewer

Length: 1314 bp
ATGGAGGTGGTGACGCGACTCACGCAAGTACTTCACATGAACAAAGGCGATGGTGAGACTAGTTATGCCAGAAACTCCAGTGTTCAGAGAAAAACAATATCTATCGCCAAGCCAATCATTGAGGAAGCAGTACTAGAAATCTTGGGCTCAAATGTCATGGGAATTGAAAGCATGGGAATAGCAGATTTGGATCATCGATGTCATCCGTCACCAGTAACAGAGCTTAGAGTATATCTAAATGACCTCTTTAGCAACGACTTCAACTCCGTCTTTATGTCACTCCCATCATTCTACAACAAACTGAGACAAGACTATGATAATGGTCGTGATCCGGATCAGCATGTCATACATCCCTTGATTTCTGCTGTGCCCGGTTCGTTTTATGGTCCACTATTTCCTAAAAAGAGTTTGCACTTTGTTCACTCTTCTTTTAGTCTTCACTGGCTCTCTCAGATCCCCGATGGCCTAAACAACAAAGGCAAGATTTACATTTCTAAGAGCAGCCCAGAGTGTGTGTTGGATGCATACTCCATGCAGTTTCAGAAAGACTTTTCGGTTTTTCTGAGGTCTAGGGCACAAGAAATAGTTAACGGAGGTCGAATGGGCCTTATAGAAGAGGAAATGGTTGATTCCTTCGACATGCCCTACTATACTTCATGTTCCTTCGACATGCCCTACTATACTTCATGTGCAGAGGAATTGAGTTTGGTGTTACAAAAAGAAGGGTCTTTCATCAAGGACCGCCTTGAAGCATTCGAACTGAATTGGGATACTGGTGCTGCTGATCTCGATCACAACATGCCTAACGTTAGGCCTAACGGTGCATTTGACGATGATCATACAATAACAAGCAGCGGGCAGCGAGTGGCCAGTAGCATAAGAGTCGTGGCTGAGTCAATGCTTAAATCTCATTTTGGCAAAGAGATAATGGATGAGGTGTTTCAGAAATATGCAGAGCTCGTTAGCAAGCACTTATCAAATAGTACCAGACCCAATTATACGCACCTGGATCTCGCCGTTGTGGTCAAACATATCATGGCACTTGACGACGTTAAGGTTATCGACGTCGCGGAGGATTTGGATCTCGCGACAGATTTGACCGTGCCGCAGGCTCGGTTCCCAATCCGGTTTGCCCGCTCTAGCTCGGAGAAGTTGATCATCATGGCCTCGGACCTTGACAAAGCTGAGCTCGGCCCCTGCTGCAGAGGCAACTGCATGGCCAGAGACGTGTCCCCCCTCGACATCGGTAGACTCAAGTCAAGACGACGCCGTGACTTCCCCGACGAGTCCTTTATGGCGGCCACATCTCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00592 ko01110 map00592 map01110
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

438

Amino Acids

49.17

Weight (kDa)

5.41

Isoelectric Point (pI)

50.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 68 - 203 7.4e-52 SAM dependent carboxyl methyltransferase
Methyltransf_7 PF03492 202 - 335 1.4e-20 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1068
AccBSI CCGCTC 1 cut(s) 1137
AccI GTMKAC 1 cut(s) 1249
AccII CGCG 3 cut(s) 16, 1070, 1088
AccIII TCCGGA 1 cut(s) 331
AciI CCGC 6 cut(s) 742, 855, 1070, 1106, 1135, 1298
AclI AACGTT 1 cut(s) 807
AclWI GGATC 6 cut(s) 198, 323, 342, 448, 1017, 1089
AcoI YGGCCR 3 cut(s) 867, 1218, 1299
AcyI GRCGYC 2 cut(s) 1065, 1267
AfaI GTAC 3 cut(s) 30, 132, 985
AflIII ACRYGT 1 cut(s) 1227
AgsI TTSAA 3 cut(s) 167, 262, 749
AhlI ACTAGT 1 cut(s) 59
AjiI CACGTC 1 cut(s) 1228
AjnI CCWGG 1 cut(s) 1005
AluBI AGCT 5 cut(s) 223, 958, 1143, 1184, 1189
AluI AGCT 5 cut(s) 223, 958, 1143, 1184, 1189
Alw21I GWGCWC 2 cut(s) 960, 1191
Alw26I GTCTC 3 cut(s) 50, 298, 1218
AlwI GGATC 6 cut(s) 198, 323, 342, 448, 1017, 1089
Aor13HI TCCGGA 1 cut(s) 331
AoxI GGCC 8 cut(s) 463, 604, 812, 867, 1164, 1192, 1218, 1299
ApeKI GCWGC 5 cut(s) 501, 779, 852, 859, 1200
AspS9I GGNCC 5 cut(s) 386, 604, 739, 1171, 1193
AsuC2I CCSGG 1 cut(s) 372
AsuHPI GGTGA 3 cut(s) 22, 65, 201
AsuII TTCGAA 1 cut(s) 756
AvaII GGWCC 3 cut(s) 386, 739, 1171
BaeGI GKGCMC 2 cut(s) 372, 577
BalI TGGCCA 2 cut(s) 869, 1220
BanII GRGCYC 3 cut(s) 149, 960, 1191
BbsI GAAGAC 1 cut(s) 428
Bbv12I GWGCWC 2 cut(s) 960, 1191
BbvI GCAGC 5 cut(s) 513, 766, 864, 871, 1187
BccI CCATC 3 cut(s) 44, 292, 455
BceAI ACGGC 2 cut(s) 1001, 1254
BcgI CGANNNNNNTGC 2 cut(s) 865, 899
BciT130I CCWGG 1 cut(s) 1007
BciVI GTATCC 1 cut(s) 763
BclI TGATCA 2 cut(s) 835, 1155
BcnI CCSGG 1 cut(s) 372
BcoDI GTCTC 3 cut(s) 50, 298, 1218
BcuI ACTAGT 1 cut(s) 59
BfaI CTAG 4 cut(s) 60, 134, 570, 1140
BfmI CTRYAG 1 cut(s) 1201
BfuI GTATCC 1 cut(s) 763
BisI GCNGC 7 cut(s) 502, 780, 853, 860, 1106, 1201, 1299
BlpI GCTNAGC 1 cut(s) 1185
BlsI GCNGC 7 cut(s) 503, 781, 854, 861, 1107, 1202, 1300
BmcAI AGTACT 2 cut(s) 30, 132
Bme1390I CCNGG 2 cut(s) 372, 1007
Bme18I GGWCC 3 cut(s) 386, 739, 1171
BmgBI CACGTC 1 cut(s) 1228
BmgT120I GGNCC 5 cut(s) 386, 604, 739, 1171, 1193
BmiI GGNNCC 2 cut(s) 1118, 1195
BmrFI CCNGG 2 cut(s) 372, 1007
BmsI GCATC 1 cut(s) 511
BoxI GACNNNNGTC 1 cut(s) 1255
BpiI GAAGAC 1 cut(s) 428
BpmI CTGGAG 1 cut(s) 61
Bpu1102I GCTNAGC 1 cut(s) 1185
Bpu14I TTCGAA 1 cut(s) 756
BpuEI CTTGAG 1 cut(s) 1238
BpuMI CCSGG 1 cut(s) 372
Bsa29I ATCGAT 1 cut(s) 196
BsaBI GATNNNNATC 4 cut(s) 189, 333, 789, 1080
BsaHI GRCGYC 2 cut(s) 1065, 1267
BsaJI CCNNGG 1 cut(s) 1167
BsaWI WCCGGW 2 cut(s) 331, 1125
BsaXI ACNNNNNCTCC 2 cut(s) 248, 278
Bse1I ACTGG 5 cut(s) 78, 212, 446, 778, 870
Bse8I GATNNNNATC 4 cut(s) 189, 333, 789, 1080
BseAI TCCGGA 1 cut(s) 331
BseBI CCWGG 1 cut(s) 1007
BseCI ATCGAT 1 cut(s) 196
BseDI CCNNGG 1 cut(s) 1167
BseGI GGATG 4 cut(s) 202, 349, 526, 937
BseJI GATNNNNATC 4 cut(s) 189, 333, 789, 1080
BseMII CTCAG 5 cut(s) 293, 464, 554, 882, 1176
BseNI ACTGG 5 cut(s) 78, 212, 446, 778, 870
BseSI GKGCMC 2 cut(s) 372, 577
BseXI GCAGC 5 cut(s) 513, 766, 864, 871, 1187
BsgI GTGCAG 1 cut(s) 711
Bsh1236I CGCG 3 cut(s) 16, 1070, 1088
BshFI GGCC 8 cut(s) 465, 606, 814, 869, 1166, 1194, 1220, 1301
BshVI ATCGAT 1 cut(s) 196
BsiHKAI GWGCWC 2 cut(s) 960, 1191
BsiSI CCGG 3 cut(s) 332, 372, 1126
BslFI GGGAC 1 cut(s) 1216
BsmAI GTCTC 3 cut(s) 50, 298, 1218
BsmBI CGTCTC 1 cut(s) 1218
BsmFI GGGAC 1 cut(s) 1216
BsmI GAATGC 1 cut(s) 752
BsnI GGCC 8 cut(s) 465, 606, 814, 869, 1166, 1194, 1220, 1301
Bsp119I TTCGAA 1 cut(s) 756
Bsp1286I GDGCHC 5 cut(s) 149, 372, 577, 960, 1191
Bsp13I TCCGGA 1 cut(s) 331
Bsp1720I GCTNAGC 1 cut(s) 1185
Bsp68I TCGCGA 1 cut(s) 1088
BspACI CCGC 6 cut(s) 742, 855, 1070, 1106, 1135, 1298
BspANI GGCC 8 cut(s) 465, 606, 814, 869, 1166, 1194, 1220, 1301
BspCNI CTCAG 5 cut(s) 294, 463, 555, 883, 1177
BspDI ATCGAT 1 cut(s) 196
BspEI TCCGGA 1 cut(s) 331
BspFNI CGCG 3 cut(s) 16, 1070, 1088
BspLI GGNNCC 2 cut(s) 1118, 1195
BspMAI CTGCAG 1 cut(s) 1205
BspPI GGATC 6 cut(s) 198, 323, 342, 448, 1017, 1089
BspT104I TTCGAA 1 cut(s) 756
BsrBI CCGCTC 1 cut(s) 1137
BsrI ACTGG 5 cut(s) 78, 212, 446, 778, 870
BssECI CCNNGG 1 cut(s) 1167
BssNI GRCGYC 2 cut(s) 1065, 1267
Bst2UI CCWGG 1 cut(s) 1007
Bst4CI ACNGT 2 cut(s) 821, 1102
Bst6I CTCTTC 2 cut(s) 430, 609
BstACI GRCGYC 2 cut(s) 1065, 1267
BstBI TTCGAA 1 cut(s) 756
BstC8I GCNNGC 4 cut(s) 857, 968, 1110, 1135
BstDEI CTNAG 7 cut(s) 224, 302, 450, 495, 563, 891, 1185
BstF5I GGATG 4 cut(s) 202, 349, 526, 937
BstFNI CGCG 3 cut(s) 16, 1070, 1088
BstMAI GTCTC 3 cut(s) 50, 298, 1218
BstMWI GCNNNNNNNGC 3 cut(s) 22, 1200, 1206
BstNI CCWGG 1 cut(s) 1007
BstNSI RCATGY 4 cut(s) 344, 643, 673, 802
BstPAI GACNNNNGTC 1 cut(s) 1255
BstSCI CCNGG 2 cut(s) 370, 1005
BstSFI CTRYAG 1 cut(s) 1201
BstSLI GKGCMC 2 cut(s) 372, 577
BstUI CGCG 3 cut(s) 16, 1070, 1088
BstV1I GCAGC 5 cut(s) 513, 766, 864, 871, 1187
BstV2I GAAGAC 1 cut(s) 428
BstX2I RGATCY 3 cut(s) 453, 1009, 1081
BstYI RGATCY 3 cut(s) 453, 1009, 1081
Bsu15I ATCGAT 1 cut(s) 196
BsuI GTATCC 1 cut(s) 763
BsuRI GGCC 8 cut(s) 465, 606, 814, 869, 1166, 1194, 1220, 1301
BsuTUI ATCGAT 1 cut(s) 196
BtgZI GCGATG 1 cut(s) 63
BtrI CACGTC 1 cut(s) 1228
BtsCI GGATG 4 cut(s) 202, 349, 526, 937
BtsIMutI CAGTG 2 cut(s) 85, 439
BtuMI TCGCGA 1 cut(s) 1088
Cac8I GCNNGC 4 cut(s) 857, 968, 1110, 1135
Cfr13I GGNCC 5 cut(s) 386, 604, 739, 1171, 1193
ClaI ATCGAT 1 cut(s) 196
CseI GACGC 2 cut(s) 22, 1275
Csp6I GTAC 3 cut(s) 29, 131, 984
CviQI GTAC 3 cut(s) 29, 131, 984
DdeI CTNAG 7 cut(s) 224, 302, 450, 495, 563, 891, 1185
EaeI YGGCCR 3 cut(s) 867, 1218, 1299
Eam1104I CTCTTC 2 cut(s) 430, 609
EarI CTCTTC 2 cut(s) 430, 609
Ecl136II GAGCTC 2 cut(s) 958, 1189
Eco147I AGGCCT 1 cut(s) 814
Eco24I GRGCYC 3 cut(s) 149, 960, 1191
Eco47I GGWCC 3 cut(s) 386, 739, 1171
Eco53kI GAGCTC 2 cut(s) 958, 1189
EcoICRI GAGCTC 2 cut(s) 958, 1189
EcoRII CCWGG 1 cut(s) 1005
EcoT22I ATGCAT 1 cut(s) 526
EcoT38I GRGCYC 3 cut(s) 149, 960, 1191
Esp3I CGTCTC 1 cut(s) 1218
FaqI GGGAC 1 cut(s) 1216
FauI CCCGC 2 cut(s) 848, 1142
FbaI TGATCA 2 cut(s) 835, 1155
FblI GTMKAC 1 cut(s) 1249
Fnu4HI GCNGC 7 cut(s) 502, 780, 853, 860, 1106, 1201, 1299
FokI GGATG 4 cut(s) 189, 336, 533, 944
FriOI GRGCYC 3 cut(s) 149, 960, 1191
Fsp4HI GCNGC 7 cut(s) 502, 780, 853, 860, 1106, 1201, 1299
FspBI CTAG 4 cut(s) 60, 134, 570, 1140
GluI GCNGC 7 cut(s) 502, 780, 853, 860, 1106, 1201, 1299
GsuI CTGGAG 1 cut(s) 61
HaeIII GGCC 8 cut(s) 465, 606, 814, 869, 1166, 1194, 1220, 1301
HapII CCGG 3 cut(s) 332, 372, 1126
HgaI GACGC 2 cut(s) 22, 1275
Hin1I GRCGYC 2 cut(s) 1065, 1267
HincII GTYRAC 1 cut(s) 589
HindII GTYRAC 1 cut(s) 589
HinfI GANTC 6 cut(s) 18, 629, 882, 893, 1251, 1286
HpaI GTTAAC 1 cut(s) 589
HpaII CCGG 3 cut(s) 332, 372, 1126
HphI GGTGA 3 cut(s) 22, 65, 201
Hpy166II GTNNAC 4 cut(s) 389, 421, 589, 1250
Hpy188I TCNGA 7 cut(s) 87, 453, 543, 564, 945, 1147, 1171
Hpy188III TCNNGA 5 cut(s) 326, 332, 788, 1087, 1260
Hpy8I GTNNAC 4 cut(s) 389, 421, 589, 1250
Hpy99I CGWCG 5 cut(s) 1052, 1067, 1070, 1269, 1286
HpyAV CCTTC 3 cut(s) 643, 673, 716
HpyCH4III ACNGT 2 cut(s) 821, 1102
HpyCH4IV ACGT 4 cut(s) 807, 1050, 1065, 1227
HpyCH4V TGCA 8 cut(s) 412, 524, 535, 692, 824, 953, 1203, 1215
HpyF10VI GCNNNNNNNGC 3 cut(s) 22, 1200, 1206
HpyF3I CTNAG 7 cut(s) 224, 302, 450, 495, 563, 891, 1185
HpySE526I ACGT 4 cut(s) 807, 1050, 1065, 1227
Hsp92I GRCGYC 2 cut(s) 1065, 1267
Kpn2I TCCGGA 1 cut(s) 331
Ksp22I TGATCA 2 cut(s) 835, 1155
KspAI GTTAAC 1 cut(s) 589
Lsp1109I GCAGC 5 cut(s) 513, 766, 864, 871, 1187
LweI GCATC 1 cut(s) 511
MaeI CTAG 4 cut(s) 60, 134, 570, 1140
MaeII ACGT 4 cut(s) 807, 1050, 1065, 1227
MaeIII GTNAC 6 cut(s) 10, 207, 214, 276, 711, 1271
MbiI CCGCTC 1 cut(s) 1137
MboII GAAGA 3 cut(s) 417, 428, 626
MflI RGATCY 3 cut(s) 453, 1009, 1081
MhlI GDGCHC 5 cut(s) 149, 372, 577, 960, 1191
MlsI TGGCCA 2 cut(s) 869, 1220
MluCI AATT 4 cut(s) 162, 698, 763, 994
MluNI TGGCCA 2 cut(s) 869, 1220
MlyI GAGTC 5 cut(s) 12, 891, 902, 1245, 1295
MmeI TCCRAC 1 cut(s) 498
Mox20I TGGCCA 2 cut(s) 869, 1220
Mph1103I ATGCAT 1 cut(s) 526
MroI TCCGGA 1 cut(s) 331
MscI TGGCCA 2 cut(s) 869, 1220
MseI TTAA 3 cut(s) 588, 903, 1053
Msp20I TGGCCA 2 cut(s) 869, 1220
MspA1I CMGCKG 1 cut(s) 855
MspI CCGG 3 cut(s) 332, 372, 1126
MspR9I CCNGG 2 cut(s) 372, 1007
Mva1269I GAATGC 1 cut(s) 752
MvaI CCWGG 1 cut(s) 1007
MvnI CGCG 3 cut(s) 16, 1070, 1088
MwoI GCNNNNNNNGC 3 cut(s) 22, 1200, 1206
NciI CCSGG 1 cut(s) 372
NlaIV GGNNCC 2 cut(s) 1118, 1195
NmeAIII GCCGAG 1 cut(s) 1170
NmuCI GTSAC 4 cut(s) 10, 207, 276, 1271
NruI TCGCGA 1 cut(s) 1088
NsiI ATGCAT 1 cut(s) 526
NspI RCATGY 4 cut(s) 344, 643, 673, 802
NspV TTCGAA 1 cut(s) 756
PceI AGGCCT 1 cut(s) 814
PctI GAATGC 1 cut(s) 752
PfeI GAWTC 1 cut(s) 629
PkrI GCNGC 7 cut(s) 503, 781, 854, 861, 1107, 1202, 1300
PleI GAGTC 5 cut(s) 12, 890, 901, 1245, 1294
PpsI GAGTC 5 cut(s) 12, 890, 901, 1245, 1294
PshAI GACNNNNGTC 1 cut(s) 1255
Psp124BI GAGCTC 2 cut(s) 960, 1191
Psp1406I AACGTT 1 cut(s) 807
Psp6I CCWGG 1 cut(s) 1005
PspGI CCWGG 1 cut(s) 1005
PspN4I GGNNCC 2 cut(s) 1118, 1195
PspPI GGNCC 5 cut(s) 386, 604, 739, 1171, 1193
PstI CTGCAG 1 cut(s) 1205
PsuI RGATCY 3 cut(s) 453, 1009, 1081
RruI TCGCGA 1 cut(s) 1088
RsaI GTAC 3 cut(s) 30, 132, 985
RsaNI GTAC 3 cut(s) 29, 131, 984
SacI GAGCTC 2 cut(s) 960, 1191
SaqAI TTAA 3 cut(s) 588, 903, 1053
SatI GCNGC 7 cut(s) 502, 780, 853, 860, 1106, 1201, 1299
Sau96I GGNCC 5 cut(s) 386, 604, 739, 1171, 1193
ScaI AGTACT 2 cut(s) 30, 132
SchI GAGTC 5 cut(s) 12, 891, 902, 1245, 1295
ScrFI CCNGG 2 cut(s) 372, 1007
SduI GDGCHC 5 cut(s) 149, 372, 577, 960, 1191
SfaNI GCATC 1 cut(s) 511
SfcI CTRYAG 1 cut(s) 1201
SfuI TTCGAA 1 cut(s) 756
SinI GGWCC 3 cut(s) 386, 739, 1171
SmlI CTYRAG 1 cut(s) 1253
SmoI CTYRAG 1 cut(s) 1253
SpeI ACTAGT 1 cut(s) 59
Sse9I AATT 4 cut(s) 162, 698, 763, 994
SseBI AGGCCT 1 cut(s) 814
SsiI CCGC 6 cut(s) 742, 855, 1070, 1106, 1135, 1298
SspMI CTAG 4 cut(s) 60, 134, 570, 1140
SstI GAGCTC 2 cut(s) 960, 1191
StuI AGGCCT 1 cut(s) 814
StyD4I CCNGG 2 cut(s) 370, 1005
TaaI ACNGT 2 cut(s) 821, 1102
TaiI ACGT 4 cut(s) 810, 1053, 1068, 1230
TaqI TCGA 8 cut(s) 196, 598, 636, 666, 756, 789, 1062, 1239
TasI AATT 4 cut(s) 162, 698, 763, 994
TatI WGTACW 2 cut(s) 28, 130
TauI GCSGC 2 cut(s) 1108, 1301
TfiI GAWTC 1 cut(s) 629
Tru1I TTAA 3 cut(s) 588, 903, 1053
Tru9I TTAA 3 cut(s) 588, 903, 1053
TscAI CASTG 2 cut(s) 85, 446
TseFI GTSAC 4 cut(s) 10, 207, 276, 1271
TseI GCWGC 5 cut(s) 501, 779, 852, 859, 1200
Tsp45I GTSAC 4 cut(s) 10, 207, 276, 1271
TspDTI ATGAA 4 cut(s) 53, 645, 675, 721
TspGWI ACGGA 3 cut(s) 195, 256, 606
TspRI CASTG 2 cut(s) 85, 446
VpaK11BI GGWCC 3 cut(s) 386, 739, 1171
XceI RCATGY 4 cut(s) 344, 643, 673, 802
XmiI GTMKAC 1 cut(s) 1249
XspI CTAG 4 cut(s) 60, 134, 570, 1140
ZraI GACGTC 1 cut(s) 1066
ZrmI AGTACT 2 cut(s) 30, 132
Zsp2I ATGCAT 1 cut(s) 526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.