Rroxscaffold_5G00382280

Jasmonate O-methyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001051
Physical Location & Seq
Reverse (-)
62111178 .. 62117136
5959 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00382280.1

Sequence Viewer

Length: 1299 bp
ATGGAGGTGACGCAAATACTTCACATGAACAACGGAGATGACGAGACTAGTTATGCCAAAAACTCTACAGTTCAGAGAAAAACATTATCTATAACCAAGCCGATCATTGAGGAAGCAGTGCTAGAACTCTTGGGATACAATATCATGGCAATAGAGAGCATGGGAATAGCAGATTTGGGTTGCTCATCCGGACCCAACACCTTACTACTCATCTCGCAGATCATGGATGCCATACATGCTAAACCTAGCAAAGTTACAGAGCTTAGAGTGTTTCTAAATGACCTCTTTAGCAACGACTTCAACTCCATCTTCATGTCACTTCCATCATTCTACAACAAACTGAAACAACACTATGATAATAGTCGTGATCCCGATCAGCATGTCATACATCCCTTGATTTCTGCTGTGCCCGGTTCGTTTTACGGTCCATTGTTTCCCAGAAAGAGCGTGCACTTTGTTCATTCTTCTTTTAGTCTTCACTGGCTCTCTCAGGTGCCCGATGGCCTAAACAACAAAGGAAATATATACATTTCTAAGAGCAGCCCGCAGTGCGTATTAGACGCATACTCCATGCAGTTTCAGAAAGACTTTTCGGTTTTTCTGAGGTCTAGGGCACAAGAAATAGCCGAGGGAGGACGAATGGTGTTGTCCTTCATGGGCAGGCCATCCACTGACCCAACAGCTGATCAGGGCACTTTCTACCAGTTCGAGCTCTTAGCCCACGCATTAATGGACACGGTGTCCAAGGGTCTTATAAAAGAGGAAATGGTTGATTCCTTCAACATGCCCTACTATACTTCATGTGCAGAGGAATTGAATTTGGTGTTACAAAAAGAAGGGTCTTTCATCAAGGACCGCCTTGAAGCATTCGAAGTGAATTGGGATACTGGTGCTGCTGATCTCGATCACAACATGCCTAACGGTGCATTTGACCATGATCAAACAATAACAAGCAGTGGGCAGCGAGTGGCCAATAGCATAAGAGTCGTGGCCGAGTCAATGCTCGAATCTCATTTTGGAAAAGAGATAATGGATGAGGTGTTTAAGAAATATACAGAGCTCGTTAGCAAGCACTTATCAAATAGTACTACTAGACCCAAAAAACTGCAATATCTATCGCCAAGCCGATCATCGAGAGAGGAAGTAGTTCTTGAAATCTTGGGCTCCAATATCACGGGAATAGAGAGCATGGGCATAGCGGATTTGGGTTGCTCATCCGAACCCAACACCTTCCGACTCATCTCGCAGATCATGGATGTCATACATGATCATTCCGTTAGAAGAGCTGCTATTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00592 ko01110 map00592 map01110
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

432

Amino Acids

48.37

Weight (kDa)

5.45

Isoelectric Point (pI)

45.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 49 - 364 1.1e-111 SAM dependent carboxyl methyltransferase
Methyltransf_7 PF03492 394 - 425 2e-07 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 755
AccB1I GGYRCC 1 cut(s) 493
AccIII TCCGGA 1 cut(s) 188
AciI CCGC 3 cut(s) 545, 856, 1199
AclWI GGATC 1 cut(s) 362
AcoI YGGCCR 2 cut(s) 969, 990
AcsI RAATTY 1 cut(s) 817
AfaI GTAC 1 cut(s) 1087
AgsI TTSAA 5 cut(s) 301, 781, 817, 863, 1154
AhdI GACNNNNNGTC 1 cut(s) 739
AhlI ACTAGT 1 cut(s) 47
AjuI GAANNNNNNNTTGG 2 cut(s) 999, 1031
AluBI AGCT 5 cut(s) 262, 683, 712, 1060, 1286
AluI AGCT 5 cut(s) 262, 683, 712, 1060, 1286
Alw21I GWGCWC 3 cut(s) 453, 714, 1062
Alw26I GTCTC 1 cut(s) 38
Alw44I GTGCAC 1 cut(s) 449
AlwI GGATC 1 cut(s) 362
Aor13HI TCCGGA 1 cut(s) 188
AoxI GGCC 4 cut(s) 502, 662, 969, 990
ApaLI GTGCAC 1 cut(s) 449
ApeKI GCWGC 4 cut(s) 540, 893, 961, 1286
ApoI RAATTY 1 cut(s) 817
AseI ATTAAT 2 cut(s) 728, 1293
Asp700I GAANNNNTTC 1 cut(s) 1146
AspS9I GGNCC 3 cut(s) 191, 425, 853
AsuC2I CCSGG 1 cut(s) 411
AsuHPI GGTGA 1 cut(s) 19
AsuII TTCGAA 1 cut(s) 870
AvaII GGWCC 3 cut(s) 191, 425, 853
BaeGI GKGCMC 5 cut(s) 411, 453, 498, 616, 695
BalI TGGCCA 1 cut(s) 971
BanI GGYRCC 1 cut(s) 493
BanII GRGCYC 3 cut(s) 714, 1062, 1166
BbsI GAAGAC 1 cut(s) 467
Bbv12I GWGCWC 3 cut(s) 453, 714, 1062
BbvI GCAGC 4 cut(s) 552, 880, 973, 1273
BccI CCATC 4 cut(s) 314, 331, 494, 673
BcgI CGANNNNNNTGC 2 cut(s) 967, 1001
BciVI GTATCC 2 cut(s) 128, 877
BclI TGATCA 3 cut(s) 685, 937, 1267
BcnI CCSGG 1 cut(s) 411
BcoDI GTCTC 1 cut(s) 38
BcuI ACTAGT 1 cut(s) 47
BfaI CTAG 5 cut(s) 48, 122, 246, 609, 1092
BfmI CTRYAG 1 cut(s) 66
BfuI GTATCC 2 cut(s) 128, 877
BisI GCNGC 4 cut(s) 541, 894, 962, 1287
BlsI GCNGC 4 cut(s) 542, 895, 963, 1288
BmcAI AGTACT 1 cut(s) 1087
Bme1390I CCNGG 1 cut(s) 411
Bme18I GGWCC 3 cut(s) 191, 425, 853
BmeRI GACNNNNNGTC 1 cut(s) 739
BmgT120I GGNCC 3 cut(s) 191, 425, 853
BmiI GGNNCC 3 cut(s) 193, 495, 1165
BmrFI CCNGG 1 cut(s) 411
BmsI GCATC 1 cut(s) 217
BpiI GAAGAC 1 cut(s) 467
Bpu14I TTCGAA 1 cut(s) 870
BpuMI CCSGG 1 cut(s) 411
BsaBI GATNNNNATC 2 cut(s) 372, 903
BsaJI CCNNGG 2 cut(s) 627, 744
BsaWI WCCGGW 1 cut(s) 188
BsaXI ACNNNNNCTCC 4 cut(s) 287, 317, 551, 581
Bse1I ACTGG 3 cut(s) 485, 703, 892
Bse8I GATNNNNATC 2 cut(s) 372, 903
BseAI TCCGGA 1 cut(s) 188
BseDI CCNNGG 2 cut(s) 627, 744
BseGI GGATG 7 cut(s) 185, 232, 388, 665, 1039, 1214, 1261
BseJI GATNNNNATC 2 cut(s) 372, 903
BseMII CTCAG 2 cut(s) 503, 593
BseNI ACTGG 3 cut(s) 485, 703, 892
BseSI GKGCMC 5 cut(s) 411, 453, 498, 616, 695
BseXI GCAGC 4 cut(s) 552, 880, 973, 1273
BsgI GTGCAG 1 cut(s) 825
BshFI GGCC 4 cut(s) 504, 664, 971, 992
BshNI GGYRCC 1 cut(s) 493
BsiHKAI GWGCWC 3 cut(s) 453, 714, 1062
BsiSI CCGG 2 cut(s) 189, 411
BsmAI GTCTC 1 cut(s) 38
BsmI GAATGC 1 cut(s) 866
BsnI GGCC 4 cut(s) 504, 664, 971, 992
Bsp119I TTCGAA 1 cut(s) 870
Bsp1286I GDGCHC 8 cut(s) 411, 453, 498, 616, 695, 714, 1062, 1166
Bsp13I TCCGGA 1 cut(s) 188
BspACI CCGC 3 cut(s) 545, 856, 1199
BspANI GGCC 4 cut(s) 504, 664, 971, 992
BspCNI CTCAG 2 cut(s) 502, 594
BspEI TCCGGA 1 cut(s) 188
BspLI GGNNCC 3 cut(s) 193, 495, 1165
BspPI GGATC 1 cut(s) 362
BspQI GCTCTTC 1 cut(s) 1276
BspT104I TTCGAA 1 cut(s) 870
BspT107I GGYRCC 1 cut(s) 493
BsrI ACTGG 3 cut(s) 485, 703, 892
BssECI CCNNGG 2 cut(s) 627, 744
BssT1I CCWWGG 1 cut(s) 744
Bst4CI ACNGT 4 cut(s) 70, 425, 739, 923
Bst6I CTCTTC 1 cut(s) 1276
BstBI TTCGAA 1 cut(s) 870
BstC8I GCNNGC 4 cut(s) 449, 545, 662, 1070
BstDEI CTNAG 5 cut(s) 263, 489, 534, 602, 715
BstF5I GGATG 7 cut(s) 185, 232, 388, 665, 1039, 1214, 1261
BstMAI GTCTC 1 cut(s) 38
BstMWI GCNNNNNNNGC 2 cut(s) 236, 549
BstNSI RCATGY 4 cut(s) 239, 383, 787, 916
BstSCI CCNGG 1 cut(s) 409
BstSFI CTRYAG 1 cut(s) 66
BstSLI GKGCMC 5 cut(s) 411, 453, 498, 616, 695
BstV1I GCAGC 4 cut(s) 552, 880, 973, 1273
BstV2I GAAGAC 1 cut(s) 467
BsuI GTATCC 2 cut(s) 128, 877
BsuRI GGCC 4 cut(s) 504, 664, 971, 992
BtsCI GGATG 7 cut(s) 185, 232, 388, 665, 1039, 1214, 1261
BtsI GCAGTG 3 cut(s) 123, 554, 961
BtsIMutI CAGTG 5 cut(s) 123, 478, 554, 669, 961
Cac8I GCNNGC 4 cut(s) 449, 545, 662, 1070
Cfr13I GGNCC 3 cut(s) 191, 425, 853
CseI GACGC 2 cut(s) 19, 569
Csp6I GTAC 1 cut(s) 1086
CviQI GTAC 1 cut(s) 1086
DdeI CTNAG 5 cut(s) 263, 489, 534, 602, 715
DriI GACNNNNNGTC 1 cut(s) 739
EaeI YGGCCR 2 cut(s) 969, 990
Eam1104I CTCTTC 1 cut(s) 1276
Eam1105I GACNNNNNGTC 1 cut(s) 739
EarI CTCTTC 1 cut(s) 1276
Ecl136II GAGCTC 2 cut(s) 712, 1060
Eco130I CCWWGG 1 cut(s) 744
Eco24I GRGCYC 3 cut(s) 714, 1062, 1166
Eco47I GGWCC 3 cut(s) 191, 425, 853
Eco53kI GAGCTC 2 cut(s) 712, 1060
EcoICRI GAGCTC 2 cut(s) 712, 1060
EcoT14I CCWWGG 1 cut(s) 744
EcoT38I GRGCYC 3 cut(s) 714, 1062, 1166
ErhI CCWWGG 1 cut(s) 744
FalI AAGNNNNNCTT 2 cut(s) 1134, 1166
FauI CCCGC 1 cut(s) 552
FbaI TGATCA 3 cut(s) 685, 937, 1267
Fnu4HI GCNGC 4 cut(s) 541, 894, 962, 1287
FokI GGATG 7 cut(s) 172, 239, 375, 652, 1046, 1201, 1268
FriOI GRGCYC 3 cut(s) 714, 1062, 1166
Fsp4HI GCNGC 4 cut(s) 541, 894, 962, 1287
FspBI CTAG 5 cut(s) 48, 122, 246, 609, 1092
GluI GCNGC 4 cut(s) 541, 894, 962, 1287
HaeIII GGCC 4 cut(s) 504, 664, 971, 992
HapII CCGG 2 cut(s) 189, 411
HgaI GACGC 2 cut(s) 19, 569
HinfI GANTC 5 cut(s) 773, 984, 995, 1007, 1236
HpaII CCGG 2 cut(s) 189, 411
HphI GGTGA 1 cut(s) 19
Hpy166II GTNNAC 1 cut(s) 451
Hpy188I TCNGA 5 cut(s) 75, 582, 603, 1219, 1235
Hpy188III TCNNGA 6 cut(s) 189, 365, 371, 902, 1134, 1151
Hpy8I GTNNAC 1 cut(s) 451
HpyAV CCTTC 4 cut(s) 661, 787, 830, 1240
HpyCH4III ACNGT 4 cut(s) 70, 425, 739, 923
HpyCH4V TGCA 5 cut(s) 451, 574, 806, 926, 1108
HpyF10VI GCNNNNNNNGC 2 cut(s) 236, 549
HpyF3I CTNAG 5 cut(s) 263, 489, 534, 602, 715
Kpn2I TCCGGA 1 cut(s) 188
Ksp22I TGATCA 3 cut(s) 685, 937, 1267
LguI GCTCTTC 1 cut(s) 1276
LmnI GCTCC 1 cut(s) 1169
LpnPI CCDG 9 cut(s) 202, 424, 451, 466, 476, 646, 674, 716, 873
Lsp1109I GCAGC 4 cut(s) 552, 880, 973, 1273
LweI GCATC 1 cut(s) 217
MaeI CTAG 5 cut(s) 48, 122, 246, 609, 1092
MaeIII GTNAC 4 cut(s) 7, 253, 315, 825
MboII GAAGA 4 cut(s) 301, 456, 467, 1293
MhlI GDGCHC 8 cut(s) 411, 453, 498, 616, 695, 714, 1062, 1166
MlsI TGGCCA 1 cut(s) 971
MluCI AATT 4 cut(s) 812, 817, 877, 1294
MluNI TGGCCA 1 cut(s) 971
MlyI GAGTC 3 cut(s) 993, 1004, 1230
MmeI TCCRAC 1 cut(s) 1258
MnlI CCTC 9 cut(s) 103, 293, 597, 622, 626, 754, 802, 1030, 1132
Mox20I TGGCCA 1 cut(s) 971
MroI TCCGGA 1 cut(s) 188
MroXI GAANNNNTTC 1 cut(s) 1146
MscI TGGCCA 1 cut(s) 971
MseI TTAA 3 cut(s) 728, 1044, 1293
MslI CAYNNNNRTG 1 cut(s) 311
Msp20I TGGCCA 1 cut(s) 971
MspA1I CMGCKG 1 cut(s) 683
MspI CCGG 2 cut(s) 189, 411
MspR9I CCNGG 1 cut(s) 411
Mva1269I GAATGC 1 cut(s) 866
MwoI GCNNNNNNNGC 2 cut(s) 236, 549
NciI CCSGG 1 cut(s) 411
NlaIV GGNNCC 3 cut(s) 193, 495, 1165
NmeAIII GCCGAG 2 cut(s) 652, 1018
NmuCI GTSAC 2 cut(s) 7, 315
NspI RCATGY 4 cut(s) 239, 383, 787, 916
NspV TTCGAA 1 cut(s) 870
PciSI GCTCTTC 1 cut(s) 1276
PcsI WCGNNNNNNNCGW 1 cut(s) 39
PctI GAATGC 1 cut(s) 866
PdmI GAANNNNTTC 1 cut(s) 1146
PfeI GAWTC 2 cut(s) 773, 1007
PkrI GCNGC 4 cut(s) 542, 895, 963, 1288
PleI GAGTC 3 cut(s) 992, 1003, 1230
PpsI GAGTC 3 cut(s) 992, 1003, 1230
PshBI ATTAAT 2 cut(s) 728, 1293
PsiI TTATAA 1 cut(s) 755
Psp124BI GAGCTC 2 cut(s) 714, 1062
PspN4I GGNNCC 3 cut(s) 193, 495, 1165
PspPI GGNCC 3 cut(s) 191, 425, 853
PvuII CAGCTG 1 cut(s) 683
RsaI GTAC 1 cut(s) 1087
RsaNI GTAC 1 cut(s) 1086
RseI CAYNNNNRTG 1 cut(s) 311
SacI GAGCTC 2 cut(s) 714, 1062
SapI GCTCTTC 1 cut(s) 1276
SaqAI TTAA 3 cut(s) 728, 1044, 1293
SatI GCNGC 4 cut(s) 541, 894, 962, 1287
Sau96I GGNCC 3 cut(s) 191, 425, 853
ScaI AGTACT 1 cut(s) 1087
SchI GAGTC 3 cut(s) 993, 1004, 1230
ScrFI CCNGG 1 cut(s) 411
SduI GDGCHC 8 cut(s) 411, 453, 498, 616, 695, 714, 1062, 1166
SfaNI GCATC 1 cut(s) 217
SfcI CTRYAG 1 cut(s) 66
SfuI TTCGAA 1 cut(s) 870
SinI GGWCC 3 cut(s) 191, 425, 853
SmiMI CAYNNNNRTG 1 cut(s) 311
SpeI ACTAGT 1 cut(s) 47
Sse9I AATT 4 cut(s) 812, 817, 877, 1294
SsiI CCGC 3 cut(s) 545, 856, 1199
SspMI CTAG 5 cut(s) 48, 122, 246, 609, 1092
SstI GAGCTC 2 cut(s) 714, 1062
StyD4I CCNGG 1 cut(s) 409
StyI CCWWGG 1 cut(s) 744
TaaI ACNGT 4 cut(s) 70, 425, 739, 923
TaqI TCGA 5 cut(s) 708, 870, 903, 1005, 1133
TasI AATT 4 cut(s) 812, 817, 877, 1294
TatI WGTACW 1 cut(s) 1085
TfiI GAWTC 2 cut(s) 773, 1007
Tru1I TTAA 3 cut(s) 728, 1044, 1293
Tru9I TTAA 3 cut(s) 728, 1044, 1293
TscAI CASTG 5 cut(s) 123, 485, 554, 676, 961
TseFI GTSAC 2 cut(s) 7, 315
TseI GCWGC 4 cut(s) 540, 893, 961, 1286
Tsp45I GTSAC 2 cut(s) 7, 315
TspDTI ATGAA 6 cut(s) 41, 301, 449, 643, 789, 835
TspGWI ACGGA 2 cut(s) 48, 1264
TspRI CASTG 5 cut(s) 123, 485, 554, 676, 961
VneI GTGCAC 1 cut(s) 449
VpaK11BI GGWCC 3 cut(s) 191, 425, 853
VspI ATTAAT 2 cut(s) 728, 1293
XapI RAATTY 1 cut(s) 817
XceI RCATGY 4 cut(s) 239, 383, 787, 916
XmnI GAANNNNTTC 1 cut(s) 1146
XspI CTAG 5 cut(s) 48, 122, 246, 609, 1092
ZrmI AGTACT 1 cut(s) 1087
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.