Rw4G033250

Jasmonate O-methyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0001051
Physical Location & Seq
Reverse (-)
60335184 .. 60336920
1737 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G033250.1

Sequence Viewer

Length: 1140 bp
ATGGAGGTGACGCAAATACTTCACATGAACAACGGAGATGACGAGACTAGTTATGCCAAAAACTCTACAGTTCAGAGAAAAACATTATCCATAGCCAAGCCGATCATTGAGGAAGCAGTGTTAGAACTCTTGGGATCCGATATCATGGCAATAGAGAGCATGGGAATAGCAGATTTGGGTTGCTCATCCGGACCCAACACGTTACTACTCATCTCGCAGATCATGGATACCATACATGCTATACCTAGCAAAGTTACAGAGCTTAGATTGTTTCTAAATGACCTCTTTAGCAACGACTTCAACTCCATCTTCATGTCACTCCCATCATTCTACAACAAACTGAAACAAGACTATGATAATAGTCGTGATCCCGATCAGCATGTCATAAATCCGTTGATTTCTGCTGTGCCCGGTTCGTTTTACGGTCCATTGTTTCCCAGAAAGAGCGTGCACTTTGTTCATTCTTCTTTTAGTCTTCACTGGCTCTCTCAGGTGCCCGATGGCCTAAACAACAAAGGAAATATATACATTTCTAAGAGCAGCCCGCAGTGCGTATTAGACGCATACTCCATGCAGTTTCAGAAAGACTTTTCCGTTTTTCTGAGGTCTAGGGCACAAGAAATAGCCGAGGGAGGACGAATGGTGTTGTCCTTCATGGGCAGGCCATCCACTGACCCAACAGCTGATCAGGGCACTTTCTACCAGTTCGAGCTCTTAGCCCACGCATTAATGGACACGGTGTCCAAGGGTCTTATAAAAGAGGATATGGTTAATTCCTTCAACATGCCCTACTATACTTCATGTGCAGAGGAATTGAATTTGGTGTTACAAAAAGAAGGGTCTTTCATCAAGGACCGCCTTGAAGCATTCGAAGTGAATTGGGATACGGATGCGGCTGATCTTGATCACAACATGCCTAACGGTGCATTTGACCATGATCAAACAATAACAAGCAGTGGGCAGCGAGTGGCCAATAGCATAAGAGTCGTGGCCGAGTCAATGCTCGAATCTCATTTTGGAAAAGAGATAATGGATGAGGTGTTTAAGAAATATACAGAGCTCGTTAGCAAGCACTTATCAAATAGTACTACTAGACCCAAGTATACAGTTTTGGTCATCTCACTCATTCGAAAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00592 ko01110 map00592 map01110
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

379

Amino Acids

42.55

Weight (kDa)

5.23

Isoelectric Point (pI)

35.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 50 - 377 6.1e-112 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 755
AccB1I GGYRCC 1 cut(s) 493
AccI GTMKAC 1 cut(s) 1103
AccIII TCCGGA 1 cut(s) 188
AciI CCGC 3 cut(s) 545, 856, 893
AclWI GGATC 3 cut(s) 129, 142, 362
AcoI YGGCCR 2 cut(s) 969, 990
AcsI RAATTY 1 cut(s) 817
AfaI GTAC 1 cut(s) 1087
AflIII ACRYGT 1 cut(s) 198
AgsI TTSAA 4 cut(s) 301, 781, 817, 863
AhdI GACNNNNNGTC 1 cut(s) 739
AhlI ACTAGT 1 cut(s) 47
AjuI GAANNNNNNNTTGG 2 cut(s) 999, 1031
AluBI AGCT 4 cut(s) 262, 683, 712, 1060
AluI AGCT 4 cut(s) 262, 683, 712, 1060
Alw21I GWGCWC 3 cut(s) 453, 714, 1062
Alw26I GTCTC 1 cut(s) 38
Alw44I GTGCAC 1 cut(s) 449
AlwI GGATC 3 cut(s) 129, 142, 362
Aor13HI TCCGGA 1 cut(s) 188
AoxI GGCC 4 cut(s) 502, 662, 969, 990
ApaLI GTGCAC 1 cut(s) 449
ApeKI GCWGC 2 cut(s) 540, 961
ApoI RAATTY 1 cut(s) 817
AseI ATTAAT 1 cut(s) 728
AspS9I GGNCC 3 cut(s) 191, 425, 853
AsuC2I CCSGG 1 cut(s) 411
AsuHPI GGTGA 1 cut(s) 19
AsuII TTCGAA 2 cut(s) 870, 1129
AvaII GGWCC 3 cut(s) 191, 425, 853
BaeGI GKGCMC 5 cut(s) 411, 453, 498, 616, 695
BalI TGGCCA 1 cut(s) 971
BamHI GGATCC 1 cut(s) 134
BanI GGYRCC 1 cut(s) 493
BanII GRGCYC 2 cut(s) 714, 1062
BbsI GAAGAC 1 cut(s) 467
Bbv12I GWGCWC 3 cut(s) 453, 714, 1062
BbvI GCAGC 2 cut(s) 552, 973
BccI CCATC 4 cut(s) 314, 331, 494, 673
BcgI CGANNNNNNTGC 2 cut(s) 967, 1001
BciVI GTATCC 2 cut(s) 220, 877
BclI TGATCA 3 cut(s) 685, 904, 937
BcnI CCSGG 1 cut(s) 411
BcoDI GTCTC 1 cut(s) 38
BcuI ACTAGT 1 cut(s) 47
BfaI CTAG 4 cut(s) 48, 246, 609, 1092
BfmI CTRYAG 1 cut(s) 66
BfuI GTATCC 2 cut(s) 220, 877
BisI GCNGC 3 cut(s) 541, 894, 962
BlsI GCNGC 3 cut(s) 542, 895, 963
BmcAI AGTACT 1 cut(s) 1087
Bme1390I CCNGG 1 cut(s) 411
Bme18I GGWCC 3 cut(s) 191, 425, 853
BmeRI GACNNNNNGTC 1 cut(s) 739
BmgT120I GGNCC 3 cut(s) 191, 425, 853
BmiI GGNNCC 3 cut(s) 136, 193, 495
BmrFI CCNGG 1 cut(s) 411
BmsI GCATC 1 cut(s) 880
BpiI GAAGAC 1 cut(s) 467
Bpu14I TTCGAA 2 cut(s) 870, 1129
BpuMI CCSGG 1 cut(s) 411
BsaBI GATNNNNATC 2 cut(s) 372, 903
BsaJI CCNNGG 2 cut(s) 627, 744
BsaWI WCCGGW 1 cut(s) 188
BsaXI ACNNNNNCTCC 4 cut(s) 287, 317, 551, 581
Bse1I ACTGG 2 cut(s) 485, 703
Bse8I GATNNNNATC 2 cut(s) 372, 903
BseAI TCCGGA 1 cut(s) 188
BseDI CCNNGG 2 cut(s) 627, 744
BseGI GGATG 4 cut(s) 185, 665, 895, 1039
BseJI GATNNNNATC 2 cut(s) 372, 903
BseMII CTCAG 2 cut(s) 503, 593
BseNI ACTGG 2 cut(s) 485, 703
BseSI GKGCMC 5 cut(s) 411, 453, 498, 616, 695
BseXI GCAGC 2 cut(s) 552, 973
BsgI GTGCAG 1 cut(s) 825
BshFI GGCC 4 cut(s) 504, 664, 971, 992
BshNI GGYRCC 1 cut(s) 493
BsiHKAI GWGCWC 3 cut(s) 453, 714, 1062
BsiSI CCGG 2 cut(s) 189, 411
BsmAI GTCTC 1 cut(s) 38
BsmI GAATGC 1 cut(s) 866
BsnI GGCC 4 cut(s) 504, 664, 971, 992
Bsp119I TTCGAA 2 cut(s) 870, 1129
Bsp1286I GDGCHC 7 cut(s) 411, 453, 498, 616, 695, 714, 1062
Bsp13I TCCGGA 1 cut(s) 188
Bsp143I GATC 9 cut(s) 102, 134, 219, 367, 373, 685, 898, 904, 937
BspACI CCGC 3 cut(s) 545, 856, 893
BspANI GGCC 4 cut(s) 504, 664, 971, 992
BspCNI CTCAG 2 cut(s) 502, 594
BspEI TCCGGA 1 cut(s) 188
BspLI GGNNCC 3 cut(s) 136, 193, 495
BspPI GGATC 3 cut(s) 129, 142, 362
BspT104I TTCGAA 2 cut(s) 870, 1129
BspT107I GGYRCC 1 cut(s) 493
BsrI ACTGG 2 cut(s) 485, 703
BssECI CCNNGG 2 cut(s) 627, 744
BssMI GATC 9 cut(s) 102, 134, 219, 367, 373, 685, 898, 904, 937
BssNAI GTATAC 1 cut(s) 1104
BssT1I CCWWGG 1 cut(s) 744
Bst1107I GTATAC 1 cut(s) 1104
Bst4CI ACNGT 5 cut(s) 70, 425, 739, 923, 1108
BstBI TTCGAA 2 cut(s) 870, 1129
BstC8I GCNNGC 4 cut(s) 449, 545, 662, 1070
BstDEI CTNAG 5 cut(s) 263, 489, 534, 602, 715
BstF5I GGATG 4 cut(s) 185, 665, 895, 1039
BstKTI GATC 9 cut(s) 105, 137, 222, 370, 376, 688, 901, 907, 940
BstMAI GTCTC 1 cut(s) 38
BstMBI GATC 9 cut(s) 102, 134, 219, 367, 373, 685, 898, 904, 937
BstMWI GCNNNNNNNGC 1 cut(s) 549
BstNSI RCATGY 4 cut(s) 239, 383, 787, 916
BstSCI CCNGG 1 cut(s) 409
BstSFI CTRYAG 1 cut(s) 66
BstSLI GKGCMC 5 cut(s) 411, 453, 498, 616, 695
BstV1I GCAGC 2 cut(s) 552, 973
BstV2I GAAGAC 1 cut(s) 467
BstX2I RGATCY 1 cut(s) 134
BstYI RGATCY 1 cut(s) 134
BstZ17I GTATAC 1 cut(s) 1104
BsuI GTATCC 2 cut(s) 220, 877
BsuRI GGCC 4 cut(s) 504, 664, 971, 992
BtsCI GGATG 4 cut(s) 185, 665, 895, 1039
BtsI GCAGTG 3 cut(s) 123, 554, 961
BtsIMutI CAGTG 5 cut(s) 123, 478, 554, 669, 961
Cac8I GCNNGC 4 cut(s) 449, 545, 662, 1070
Cfr13I GGNCC 3 cut(s) 191, 425, 853
CseI GACGC 2 cut(s) 19, 569
Csp6I GTAC 1 cut(s) 1086
CviQI GTAC 1 cut(s) 1086
DdeI CTNAG 5 cut(s) 263, 489, 534, 602, 715
DpnI GATC 9 cut(s) 104, 136, 221, 369, 375, 687, 900, 906, 939
DpnII GATC 9 cut(s) 102, 134, 219, 367, 373, 685, 898, 904, 937
DriI GACNNNNNGTC 1 cut(s) 739
EaeI YGGCCR 2 cut(s) 969, 990
Eam1105I GACNNNNNGTC 1 cut(s) 739
Ecl136II GAGCTC 2 cut(s) 712, 1060
Eco130I CCWWGG 1 cut(s) 744
Eco24I GRGCYC 2 cut(s) 714, 1062
Eco32I GATATC 1 cut(s) 142
Eco47I GGWCC 3 cut(s) 191, 425, 853
Eco53kI GAGCTC 2 cut(s) 712, 1060
EcoICRI GAGCTC 2 cut(s) 712, 1060
EcoRV GATATC 1 cut(s) 142
EcoT14I CCWWGG 1 cut(s) 744
EcoT38I GRGCYC 2 cut(s) 714, 1062
ErhI CCWWGG 1 cut(s) 744
FauI CCCGC 1 cut(s) 552
FbaI TGATCA 3 cut(s) 685, 904, 937
FblI GTMKAC 1 cut(s) 1103
Fnu4HI GCNGC 3 cut(s) 541, 894, 962
FokI GGATG 4 cut(s) 172, 652, 902, 1046
FriOI GRGCYC 2 cut(s) 714, 1062
Fsp4HI GCNGC 3 cut(s) 541, 894, 962
FspBI CTAG 4 cut(s) 48, 246, 609, 1092
GluI GCNGC 3 cut(s) 541, 894, 962
HaeIII GGCC 4 cut(s) 504, 664, 971, 992
HapII CCGG 2 cut(s) 189, 411
HgaI GACGC 2 cut(s) 19, 569
HinfI GANTC 3 cut(s) 984, 995, 1007
HpaII CCGG 2 cut(s) 189, 411
HphI GGTGA 1 cut(s) 19
Hpy166II GTNNAC 2 cut(s) 451, 1104
Hpy188I TCNGA 4 cut(s) 75, 139, 582, 603
Hpy188III TCNNGA 4 cut(s) 189, 365, 371, 902
Hpy8I GTNNAC 2 cut(s) 451, 1104
HpyAV CCTTC 3 cut(s) 661, 787, 830
HpyCH4III ACNGT 5 cut(s) 70, 425, 739, 923, 1108
HpyCH4IV ACGT 1 cut(s) 200
HpyCH4V TGCA 4 cut(s) 451, 574, 806, 926
HpyF10VI GCNNNNNNNGC 1 cut(s) 549
HpyF3I CTNAG 5 cut(s) 263, 489, 534, 602, 715
HpySE526I ACGT 1 cut(s) 200
Kpn2I TCCGGA 1 cut(s) 188
Ksp22I TGATCA 3 cut(s) 685, 904, 937
Kzo9I GATC 9 cut(s) 102, 134, 219, 367, 373, 685, 898, 904, 937
LpnPI CCDG 8 cut(s) 202, 424, 451, 466, 476, 646, 674, 716
Lsp1109I GCAGC 2 cut(s) 552, 973
LweI GCATC 1 cut(s) 880
MaeI CTAG 4 cut(s) 48, 246, 609, 1092
MaeII ACGT 1 cut(s) 200
MaeIII GTNAC 5 cut(s) 7, 201, 253, 315, 825
MalI GATC 9 cut(s) 104, 136, 221, 369, 375, 687, 900, 906, 939
MboI GATC 9 cut(s) 102, 134, 219, 367, 373, 685, 898, 904, 937
MboII GAAGA 3 cut(s) 301, 456, 467
MflI RGATCY 1 cut(s) 134
MhlI GDGCHC 7 cut(s) 411, 453, 498, 616, 695, 714, 1062
MlsI TGGCCA 1 cut(s) 971
MluCI AATT 4 cut(s) 772, 812, 817, 877
MluNI TGGCCA 1 cut(s) 971
MlyI GAGTC 2 cut(s) 993, 1004
MnlI CCTC 8 cut(s) 103, 293, 597, 622, 626, 754, 802, 1030
Mox20I TGGCCA 1 cut(s) 971
MroI TCCGGA 1 cut(s) 188
MscI TGGCCA 1 cut(s) 971
MseI TTAA 3 cut(s) 728, 771, 1044
MslI CAYNNNNRTG 1 cut(s) 311
Msp20I TGGCCA 1 cut(s) 971
MspA1I CMGCKG 1 cut(s) 683
MspI CCGG 2 cut(s) 189, 411
MspR9I CCNGG 1 cut(s) 411
Mva1269I GAATGC 1 cut(s) 866
MwoI GCNNNNNNNGC 1 cut(s) 549
NciI CCSGG 1 cut(s) 411
NdeII GATC 9 cut(s) 102, 134, 219, 367, 373, 685, 898, 904, 937
NlaIV GGNNCC 3 cut(s) 136, 193, 495
NmeAIII GCCGAG 2 cut(s) 652, 1018
NmuCI GTSAC 2 cut(s) 7, 315
NspI RCATGY 4 cut(s) 239, 383, 787, 916
NspV TTCGAA 2 cut(s) 870, 1129
PcsI WCGNNNNNNNCGW 1 cut(s) 39
PctI GAATGC 1 cut(s) 866
PfeI GAWTC 1 cut(s) 1007
PkrI GCNGC 3 cut(s) 542, 895, 963
PleI GAGTC 2 cut(s) 992, 1003
PpsI GAGTC 2 cut(s) 992, 1003
PshBI ATTAAT 1 cut(s) 728
PsiI TTATAA 1 cut(s) 755
Psp124BI GAGCTC 2 cut(s) 714, 1062
PspN4I GGNNCC 3 cut(s) 136, 193, 495
PspPI GGNCC 3 cut(s) 191, 425, 853
PsuI RGATCY 1 cut(s) 134
PvuII CAGCTG 1 cut(s) 683
RsaI GTAC 1 cut(s) 1087
RsaNI GTAC 1 cut(s) 1086
RseI CAYNNNNRTG 1 cut(s) 311
SacI GAGCTC 2 cut(s) 714, 1062
SaqAI TTAA 3 cut(s) 728, 771, 1044
SatI GCNGC 3 cut(s) 541, 894, 962
Sau3AI GATC 9 cut(s) 102, 134, 219, 367, 373, 685, 898, 904, 937
Sau96I GGNCC 3 cut(s) 191, 425, 853
ScaI AGTACT 1 cut(s) 1087
SchI GAGTC 2 cut(s) 993, 1004
ScrFI CCNGG 1 cut(s) 411
SduI GDGCHC 7 cut(s) 411, 453, 498, 616, 695, 714, 1062
SfaNI GCATC 1 cut(s) 880
SfcI CTRYAG 1 cut(s) 66
SfuI TTCGAA 2 cut(s) 870, 1129
SinI GGWCC 3 cut(s) 191, 425, 853
SmiMI CAYNNNNRTG 1 cut(s) 311
SpeI ACTAGT 1 cut(s) 47
Sse9I AATT 4 cut(s) 772, 812, 817, 877
SsiI CCGC 3 cut(s) 545, 856, 893
SspMI CTAG 4 cut(s) 48, 246, 609, 1092
SstI GAGCTC 2 cut(s) 714, 1062
StyD4I CCNGG 1 cut(s) 409
StyI CCWWGG 1 cut(s) 744
TaaI ACNGT 5 cut(s) 70, 425, 739, 923, 1108
TaiI ACGT 1 cut(s) 203
TaqI TCGA 4 cut(s) 708, 870, 1005, 1129
TasI AATT 4 cut(s) 772, 812, 817, 877
TatI WGTACW 1 cut(s) 1085
TauI GCSGC 1 cut(s) 896
TfiI GAWTC 1 cut(s) 1007
Tru1I TTAA 3 cut(s) 728, 771, 1044
Tru9I TTAA 3 cut(s) 728, 771, 1044
TscAI CASTG 5 cut(s) 123, 485, 554, 676, 961
TseFI GTSAC 2 cut(s) 7, 315
TseI GCWGC 2 cut(s) 540, 961
Tsp45I GTSAC 2 cut(s) 7, 315
TspDTI ATGAA 6 cut(s) 41, 301, 449, 643, 789, 835
TspGWI ACGGA 4 cut(s) 48, 381, 583, 902
TspRI CASTG 5 cut(s) 123, 485, 554, 676, 961
VneI GTGCAC 1 cut(s) 449
VpaK11BI GGWCC 3 cut(s) 191, 425, 853
VspI ATTAAT 1 cut(s) 728
XapI RAATTY 1 cut(s) 817
XceI RCATGY 4 cut(s) 239, 383, 787, 916
XmiI GTMKAC 1 cut(s) 1103
XspI CTAG 4 cut(s) 48, 246, 609, 1092
ZrmI AGTACT 1 cut(s) 1087
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.