Rroxscaffold_3G00235790

Jasmonate O-methyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001051
Physical Location & Seq
Forward (+)
22880226 .. 22885100
4875 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00235790.1

Sequence Viewer

Length: 1350 bp
ATGGAGGTGGTGCAAGTACTTCATATGAAGAAAGGAGATGGCGAGACTAGTTATGCCAGAAACTCCACTGTTCAGAAAAAAACAATATCTATCGCCAAGCCAATCATTGAGAAAGCAGTGCTCGAAATCTTGGGCTCCAGTGTCATGGGAATAGAGAGCATGGGAATAGCAGATTTGGGTTGCTCCTCTGGACCCAACACCTTCCTACTCGTCTCGCATATCATGGATGTAATACATGCTAAATTTAGCACAGTTACAGAGCTTAGAGTGTTTCTAAATGACCTCTTTAGCAACGACTTCAACTCCATCTTTATGTCACTCCCAGCCTTCTACGATAAACTGAAGCAAGACCATAATAATGGTCGTGATCCTGATCAGCATGTCATAGATCCCTTAATTTCTGCTGTGCCCGGTTCGTTTTACGGTCCATTGTTTCCCAGAAAGAGCATGCACTTTGTTCACTCTTCTTTTAGTCTTCACTGGCTATCTCAGGTCCCCGATATCCTGAACAACAAAGGAAAGATTTACATGTCTAAGAGCAGCCCACAGTGTGTGTTGGATGCATACTCCATGCAGTTTCAGAAAGACTTTGCTGTTTTTCTGAGGTCTAGGGCACAAGAAATAGTCGACGGAGGACGAATGGTGTTGTCCTTCATGGGCAGGCCATCCTCTGACCCGACAGCTGATCAGGGAACTTTCTACCAGTACGAGCTCTTAGCCCAAGCATTAATGGCCATGGTTTCCAAGGGTCTTATAAAAGAGGAGAAGGTTGATTCCTTCAACTTGCCCCACTATGCTGCATGTGCAGAGGAATTGAATTTGGTGTTACAAAAAGAAGGGTCTTTCATCAAGGAACACCTTGAAGCATTCGAAGTTGATTGGGATACTGGCGGTGCTGATCTCGATCATCCTAACATGCCTAACGGTACATTTGACGATGATCAAACTGTCAGCAATGGGCAGCAAATGGCCAATAGCATAAGAGCCGTGGTTGAGTCAATGCTTGAATCTCATTTTGGAAAGGAGATAATGGATGCTGTGTTTCAGAAATATGCAGAGCTCGTTAGCAGGCACTTAACAAATACTACCACTATACCCAATGTCATTAATTGTGACAATCATGGAACAAACTGTAGAAATGAGGATACTAGTATGGGATTCTCGTCAGTTTACCACTCGACTCCAATTGTATTCTCTCAAGCGATGGGGGATCCCCAGGAAAACAGATATCTAGACTTTCTTGCAGCTTACTCTGCTATTAACCAGTCTTACAGCCCTGCAAAGTCCCACCAAAGTCTTATGGTCCTACACTGCTCAGGTCTACTGGGGAGGAAGAGTCTTGATATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00592 ko01110 map00592 map01110
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

449

Amino Acids

49.93

Weight (kDa)

5.47

Isoelectric Point (pI)

40.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 51 - 363 2.9e-113 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 755
AccI GTMKAC 2 cut(s) 627, 1321
AciI CCGC 1 cut(s) 891
AclWI GGATC 4 cut(s) 362, 383, 1205, 1218
AcoI YGGCCR 2 cut(s) 732, 969
AcsI RAATTY 2 cut(s) 242, 817
AcuI CTGAAG 1 cut(s) 362
AdeI CACNNNGTG 1 cut(s) 551
AfaI GTAC 3 cut(s) 18, 707, 928
AflIII ACRYGT 1 cut(s) 528
AgsI TTSAA 5 cut(s) 301, 781, 817, 863, 1007
AhlI ACTAGT 2 cut(s) 47, 1148
AjnI CCWGG 1 cut(s) 1215
AjuI GAANNNNNNNTTGG 2 cut(s) 999, 1031
AluBI AGCT 5 cut(s) 262, 683, 712, 1060, 1247
AluI AGCT 5 cut(s) 262, 683, 712, 1060, 1247
Alw21I GWGCWC 3 cut(s) 123, 714, 1062
Alw26I GTCTC 2 cut(s) 38, 217
AlwI GGATC 4 cut(s) 362, 383, 1205, 1218
AoxI GGCC 3 cut(s) 662, 732, 969
ApeKI GCWGC 4 cut(s) 540, 797, 961, 1244
ApoI RAATTY 2 cut(s) 242, 817
AseI ATTAAT 2 cut(s) 728, 1107
AspS9I GGNCC 4 cut(s) 191, 425, 493, 1303
AsuC2I CCSGG 1 cut(s) 411
AsuII TTCGAA 1 cut(s) 870
AvaII GGWCC 4 cut(s) 191, 425, 493, 1303
BaeGI GKGCMC 2 cut(s) 411, 616
BalI TGGCCA 2 cut(s) 734, 971
BamHI GGATCC 1 cut(s) 1210
BanII GRGCYC 3 cut(s) 137, 714, 1062
BbsI GAAGAC 1 cut(s) 467
Bbv12I GWGCWC 3 cut(s) 123, 714, 1062
BbvI GCAGC 4 cut(s) 552, 784, 973, 1256
BccI CCATC 4 cut(s) 32, 314, 673, 1198
BceAI ACGGC 1 cut(s) 971
BciT130I CCWGG 1 cut(s) 1217
BciVI GTATCC 2 cut(s) 877, 1138
BclI TGATCA 3 cut(s) 373, 685, 940
BcnI CCSGG 1 cut(s) 411
BcoDI GTCTC 2 cut(s) 38, 217
BcuI ACTAGT 2 cut(s) 47, 1148
BfaI CTAG 4 cut(s) 48, 609, 1149, 1232
BfmI CTRYAG 1 cut(s) 1132
BfuI GTATCC 2 cut(s) 877, 1138
BisI GCNGC 4 cut(s) 541, 798, 962, 1245
BlsI GCNGC 4 cut(s) 542, 799, 963, 1246
BmcAI AGTACT 1 cut(s) 18
Bme1390I CCNGG 2 cut(s) 411, 1217
Bme18I GGWCC 4 cut(s) 191, 425, 493, 1303
BmgT120I GGNCC 4 cut(s) 191, 425, 493, 1303
BmiI GGNNCC 4 cut(s) 136, 193, 495, 1212
BmrFI CCNGG 2 cut(s) 411, 1217
BmrI ACTGGG 1 cut(s) 1334
BmsI GCATC 2 cut(s) 550, 1024
BmuI ACTGGG 1 cut(s) 1334
BpiI GAAGAC 1 cut(s) 467
BpmI CTGGAG 1 cut(s) 121
Bpu10I CCTNAGC 1 cut(s) 1315
Bpu14I TTCGAA 1 cut(s) 870
BpuEI CTTGAG 1 cut(s) 1182
BpuMI CCSGG 1 cut(s) 411
BsaBI GATNNNNATC 2 cut(s) 372, 903
BsaJI CCNNGG 4 cut(s) 735, 744, 987, 1215
BsaXI ACNNNNNCTCC 3 cut(s) 287, 317, 1321
Bse1I ACTGG 6 cut(s) 138, 485, 703, 892, 1264, 1329
Bse3DI GCAATG 1 cut(s) 961
Bse8I GATNNNNATC 2 cut(s) 372, 903
BseBI CCWGG 1 cut(s) 1217
BseDI CCNNGG 4 cut(s) 735, 744, 987, 1215
BseGI GGATG 5 cut(s) 232, 565, 665, 907, 1039
BseJI GATNNNNATC 2 cut(s) 372, 903
BseMI GCAATG 1 cut(s) 961
BseMII CTCAG 3 cut(s) 503, 593, 1329
BseNI ACTGG 6 cut(s) 138, 485, 703, 892, 1264, 1329
BseRI GAGGAG 2 cut(s) 175, 776
BseSI GKGCMC 2 cut(s) 411, 616
BseXI GCAGC 4 cut(s) 552, 784, 973, 1256
BseYI CCCAGC 1 cut(s) 322
BsgI GTGCAG 1 cut(s) 825
BshFI GGCC 3 cut(s) 664, 734, 971
BsiHKAI GWGCWC 3 cut(s) 123, 714, 1062
BsiSI CCGG 1 cut(s) 411
BslFI GGGAC 2 cut(s) 479, 1270
BsmAI GTCTC 2 cut(s) 38, 217
BsmBI CGTCTC 1 cut(s) 217
BsmFI GGGAC 2 cut(s) 479, 1270
BsmI GAATGC 1 cut(s) 866
BsnI GGCC 3 cut(s) 664, 734, 971
Bsp119I TTCGAA 1 cut(s) 870
Bsp1286I GDGCHC 6 cut(s) 123, 137, 411, 616, 714, 1062
Bsp143I GATC 8 cut(s) 367, 373, 388, 685, 898, 904, 940, 1210
Bsp19I CCATGG 1 cut(s) 735
BspACI CCGC 1 cut(s) 891
BspANI GGCC 3 cut(s) 664, 734, 971
BspCNI CTCAG 3 cut(s) 502, 594, 1328
BspLI GGNNCC 4 cut(s) 136, 193, 495, 1212
BspPI GGATC 4 cut(s) 362, 383, 1205, 1218
BspT104I TTCGAA 1 cut(s) 870
BsrDI GCAATG 1 cut(s) 961
BsrI ACTGG 6 cut(s) 138, 485, 703, 892, 1264, 1329
BssECI CCNNGG 4 cut(s) 735, 744, 987, 1215
BssMI GATC 8 cut(s) 367, 373, 388, 685, 898, 904, 940, 1210
BssT1I CCWWGG 2 cut(s) 735, 744
Bst2UI CCWGG 1 cut(s) 1217
Bst4CI ACNGT 7 cut(s) 70, 253, 425, 549, 926, 949, 1133
Bst6I CTCTTC 2 cut(s) 469, 1328
BstBI TTCGAA 1 cut(s) 870
BstC8I GCNNGC 3 cut(s) 449, 662, 1070
BstDEI CTNAG 6 cut(s) 263, 489, 534, 602, 715, 1315
BstDSI CCRYGG 2 cut(s) 735, 987
BstF5I GGATG 5 cut(s) 232, 565, 665, 907, 1039
BstKTI GATC 8 cut(s) 370, 376, 391, 688, 901, 907, 943, 1213
BstMAI GTCTC 2 cut(s) 38, 217
BstMBI GATC 8 cut(s) 367, 373, 388, 685, 898, 904, 940, 1210
BstMWI GCNNNNNNNGC 3 cut(s) 731, 803, 1253
BstNI CCWGG 1 cut(s) 1217
BstNSI RCATGY 6 cut(s) 239, 383, 451, 532, 804, 919
BstSCI CCNGG 2 cut(s) 409, 1215
BstSFI CTRYAG 1 cut(s) 1132
BstSLI GKGCMC 2 cut(s) 411, 616
BstV1I GCAGC 4 cut(s) 552, 784, 973, 1256
BstV2I GAAGAC 1 cut(s) 467
BstX2I RGATCY 2 cut(s) 388, 1210
BstXI CCANNNNNNTGG 2 cut(s) 145, 359
BstYI RGATCY 2 cut(s) 388, 1210
BsuI GTATCC 2 cut(s) 877, 1138
BsuRI GGCC 3 cut(s) 664, 734, 971
BtgI CCRYGG 2 cut(s) 735, 987
BtgZI GCGATG 1 cut(s) 1217
BtsCI GGATG 5 cut(s) 232, 565, 665, 907, 1039
BtsI GCAGTG 2 cut(s) 123, 1309
BtsIMutI CAGTG 6 cut(s) 66, 123, 145, 478, 554, 1309
Cac8I GCNNGC 3 cut(s) 449, 662, 1070
Cfr13I GGNCC 4 cut(s) 191, 425, 493, 1303
Csp6I GTAC 3 cut(s) 17, 706, 927
CviQI GTAC 3 cut(s) 17, 706, 927
DdeI CTNAG 6 cut(s) 263, 489, 534, 602, 715, 1315
DpnI GATC 8 cut(s) 369, 375, 390, 687, 900, 906, 942, 1212
DpnII GATC 8 cut(s) 367, 373, 388, 685, 898, 904, 940, 1210
DraIII CACNNNGTG 1 cut(s) 551
EaeI YGGCCR 2 cut(s) 732, 969
Eam1104I CTCTTC 2 cut(s) 469, 1328
EarI CTCTTC 2 cut(s) 469, 1328
Ecl136II GAGCTC 2 cut(s) 712, 1060
Eco130I CCWWGG 2 cut(s) 735, 744
Eco24I GRGCYC 3 cut(s) 137, 714, 1062
Eco32I GATATC 2 cut(s) 502, 1229
Eco47I GGWCC 4 cut(s) 191, 425, 493, 1303
Eco53kI GAGCTC 2 cut(s) 712, 1060
Eco57I CTGAAG 1 cut(s) 362
EcoICRI GAGCTC 2 cut(s) 712, 1060
EcoO109I RGGNCCY 1 cut(s) 493
EcoRII CCWGG 1 cut(s) 1215
EcoRV GATATC 2 cut(s) 502, 1229
EcoT14I CCWWGG 2 cut(s) 735, 744
EcoT22I ATGCAT 1 cut(s) 565
EcoT38I GRGCYC 3 cut(s) 137, 714, 1062
ErhI CCWWGG 2 cut(s) 735, 744
Esp3I CGTCTC 1 cut(s) 217
FaqI GGGAC 2 cut(s) 479, 1270
FauNDI CATATG 1 cut(s) 24
FbaI TGATCA 3 cut(s) 373, 685, 940
FblI GTMKAC 2 cut(s) 627, 1321
Fnu4HI GCNGC 4 cut(s) 541, 798, 962, 1245
FokI GGATG 5 cut(s) 239, 572, 652, 894, 1046
FriOI GRGCYC 3 cut(s) 137, 714, 1062
Fsp4HI GCNGC 4 cut(s) 541, 798, 962, 1245
FspBI CTAG 4 cut(s) 48, 609, 1149, 1232
GluI GCNGC 4 cut(s) 541, 798, 962, 1245
GsaI CCCAGC 1 cut(s) 326
GsuI CTGGAG 1 cut(s) 121
HaeIII GGCC 3 cut(s) 664, 734, 971
HapII CCGG 1 cut(s) 411
HincII GTYRAC 1 cut(s) 628
HindII GTYRAC 1 cut(s) 628
HinfI GANTC 6 cut(s) 773, 995, 1007, 1158, 1180, 1336
HpaII CCGG 1 cut(s) 411
Hpy166II GTNNAC 4 cut(s) 460, 628, 1171, 1322
Hpy188I TCNGA 5 cut(s) 75, 582, 603, 673, 1047
Hpy188III TCNNGA 7 cut(s) 189, 365, 371, 505, 902, 1232, 1340
Hpy8I GTNNAC 4 cut(s) 460, 628, 1171, 1322
Hpy99I CGWCG 1 cut(s) 632
HpyAV CCTTC 6 cut(s) 211, 337, 661, 760, 787, 830
HpyCH4III ACNGT 7 cut(s) 70, 253, 425, 549, 926, 949, 1133
HpyCH4V TGCA 9 cut(s) 13, 451, 563, 574, 800, 806, 1055, 1244, 1280
HpyF10VI GCNNNNNNNGC 3 cut(s) 731, 803, 1253
HpyF3I CTNAG 6 cut(s) 263, 489, 534, 602, 715, 1315
Ksp22I TGATCA 3 cut(s) 373, 685, 940
Kzo9I GATC 8 cut(s) 367, 373, 388, 685, 898, 904, 940, 1210
LmnI GCTCC 2 cut(s) 140, 188
Lsp1109I GCAGC 4 cut(s) 552, 784, 973, 1256
LweI GCATC 2 cut(s) 550, 1024
MaeI CTAG 4 cut(s) 48, 609, 1149, 1232
MaeIII GTNAC 4 cut(s) 253, 315, 825, 1112
MalI GATC 8 cut(s) 369, 375, 390, 687, 900, 906, 942, 1212
MboI GATC 8 cut(s) 367, 373, 388, 685, 898, 904, 940, 1210
MboII GAAGA 4 cut(s) 40, 456, 467, 1345
MfeI CAATTG 1 cut(s) 1185
MflI RGATCY 2 cut(s) 388, 1210
MhlI GDGCHC 6 cut(s) 123, 137, 411, 616, 714, 1062
MlsI TGGCCA 2 cut(s) 734, 971
MluCI AATT 6 cut(s) 242, 396, 812, 817, 1108, 1185
MluNI TGGCCA 2 cut(s) 734, 971
MlyI GAGTC 3 cut(s) 1004, 1174, 1345
MmeI TCCRAC 1 cut(s) 537
MnlI CCTC 9 cut(s) 196, 293, 597, 626, 679, 754, 802, 1135, 1323
Mox20I TGGCCA 2 cut(s) 734, 971
Mph1103I ATGCAT 1 cut(s) 565
MscI TGGCCA 2 cut(s) 734, 971
MseI TTAA 6 cut(s) 395, 728, 1076, 1107, 1260, 1348
MslI CAYNNNNRTG 2 cut(s) 311, 357
Msp20I TGGCCA 2 cut(s) 734, 971
MspA1I CMGCKG 1 cut(s) 683
MspI CCGG 1 cut(s) 411
MspR9I CCNGG 2 cut(s) 411, 1217
MunI CAATTG 1 cut(s) 1185
Mva1269I GAATGC 1 cut(s) 866
MvaI CCWGG 1 cut(s) 1217
MwoI GCNNNNNNNGC 3 cut(s) 731, 803, 1253
NciI CCSGG 1 cut(s) 411
NcoI CCATGG 1 cut(s) 735
NdeI CATATG 1 cut(s) 24
NdeII GATC 8 cut(s) 367, 373, 388, 685, 898, 904, 940, 1210
NlaIV GGNNCC 4 cut(s) 136, 193, 495, 1212
NmuCI GTSAC 2 cut(s) 315, 1112
NsiI ATGCAT 1 cut(s) 565
NspI RCATGY 6 cut(s) 239, 383, 451, 532, 804, 919
NspV TTCGAA 1 cut(s) 870
PaeI GCATGC 1 cut(s) 451
PciI ACATGT 1 cut(s) 528
PctI GAATGC 1 cut(s) 866
PfeI GAWTC 3 cut(s) 773, 1007, 1158
PkrI GCNGC 4 cut(s) 542, 799, 963, 1246
PleI GAGTC 3 cut(s) 1003, 1174, 1344
PpsI GAGTC 3 cut(s) 1003, 1174, 1344
PpuMI RGGWCCY 1 cut(s) 493
PscI ACATGT 1 cut(s) 528
PshBI ATTAAT 2 cut(s) 728, 1107
PsiI TTATAA 1 cut(s) 755
Psp124BI GAGCTC 2 cut(s) 714, 1062
Psp5II RGGWCCY 1 cut(s) 493
Psp6I CCWGG 1 cut(s) 1215
PspFI CCCAGC 1 cut(s) 322
PspGI CCWGG 1 cut(s) 1215
PspN4I GGNNCC 4 cut(s) 136, 193, 495, 1212
PspPI GGNCC 4 cut(s) 191, 425, 493, 1303
PspPPI RGGWCCY 1 cut(s) 493
PsuI RGATCY 2 cut(s) 388, 1210
PvuII CAGCTG 1 cut(s) 683
RsaI GTAC 3 cut(s) 18, 707, 928
RsaNI GTAC 3 cut(s) 17, 706, 927
RseI CAYNNNNRTG 2 cut(s) 311, 357
SacI GAGCTC 2 cut(s) 714, 1062
SalI GTCGAC 1 cut(s) 626
SaqAI TTAA 6 cut(s) 395, 728, 1076, 1107, 1260, 1348
SatI GCNGC 4 cut(s) 541, 798, 962, 1245
Sau3AI GATC 8 cut(s) 367, 373, 388, 685, 898, 904, 940, 1210
Sau96I GGNCC 4 cut(s) 191, 425, 493, 1303
ScaI AGTACT 1 cut(s) 18
SchI GAGTC 3 cut(s) 1004, 1174, 1345
ScrFI CCNGG 2 cut(s) 411, 1217
SduI GDGCHC 6 cut(s) 123, 137, 411, 616, 714, 1062
SfaNI GCATC 2 cut(s) 550, 1024
SfcI CTRYAG 1 cut(s) 1132
SfuI TTCGAA 1 cut(s) 870
SinI GGWCC 4 cut(s) 191, 425, 493, 1303
SmiMI CAYNNNNRTG 2 cut(s) 311, 357
SmlI CTYRAG 1 cut(s) 1197
SmoI CTYRAG 1 cut(s) 1197
SpeI ACTAGT 2 cut(s) 47, 1148
SphI GCATGC 1 cut(s) 451
Sse9I AATT 6 cut(s) 242, 396, 812, 817, 1108, 1185
SsiI CCGC 1 cut(s) 891
SspMI CTAG 4 cut(s) 48, 609, 1149, 1232
SstI GAGCTC 2 cut(s) 714, 1062
StyD4I CCNGG 2 cut(s) 409, 1215
StyI CCWWGG 2 cut(s) 735, 744
TaaI ACNGT 7 cut(s) 70, 253, 425, 549, 926, 949, 1133
TaqI TCGA 5 cut(s) 123, 627, 870, 903, 1178
TasI AATT 6 cut(s) 242, 396, 812, 817, 1108, 1185
TatI WGTACW 1 cut(s) 16
TfiI GAWTC 3 cut(s) 773, 1007, 1158
Tru1I TTAA 6 cut(s) 395, 728, 1076, 1107, 1260, 1348
Tru9I TTAA 6 cut(s) 395, 728, 1076, 1107, 1260, 1348
TscAI CASTG 6 cut(s) 73, 123, 145, 485, 554, 1316
TseFI GTSAC 2 cut(s) 315, 1112
TseI GCWGC 4 cut(s) 540, 797, 961, 1244
Tsp45I GTSAC 2 cut(s) 315, 1112
TspDTI ATGAA 4 cut(s) 11, 41, 643, 835
TspGWI ACGGA 1 cut(s) 645
TspRI CASTG 6 cut(s) 73, 123, 145, 485, 554, 1316
VpaK11BI GGWCC 4 cut(s) 191, 425, 493, 1303
VspI ATTAAT 2 cut(s) 728, 1107
XapI RAATTY 2 cut(s) 242, 817
XbaI TCTAGA 1 cut(s) 1231
XceI RCATGY 6 cut(s) 239, 383, 451, 532, 804, 919
XmiI GTMKAC 2 cut(s) 627, 1321
XspI CTAG 4 cut(s) 48, 609, 1149, 1232
ZrmI AGTACT 1 cut(s) 18
Zsp2I ATGCAT 1 cut(s) 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.