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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 4 |
| AcsI | RAATTY | 2 cut(s) | 160, 168 |
| AcuI | CTGAAG | 1 cut(s) | 147 |
| AfaI | GTAC | 1 cut(s) | 70 |
| AluBI | AGCT | 1 cut(s) | 131 |
| AluI | AGCT | 1 cut(s) | 131 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 125 |
| ApoI | RAATTY | 2 cut(s) | 160, 168 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 160 |
| BanI | GGYRCC | 1 cut(s) | 4 |
| BccI | CCATC | 1 cut(s) | 92 |
| BfaI | CTAG | 1 cut(s) | 150 |
| BmiI | GGNNCC | 1 cut(s) | 6 |
| BseMII | CTCAG | 1 cut(s) | 132 |
| BshNI | GGYRCC | 1 cut(s) | 4 |
| BspCNI | CTCAG | 1 cut(s) | 131 |
| BspHI | TCATGA | 1 cut(s) | 52 |
| BspLI | GGNNCC | 1 cut(s) | 6 |
| BspT107I | GGYRCC | 1 cut(s) | 4 |
| Bst6I | CTCTTC | 1 cut(s) | 81 |
| BstDEI | CTNAG | 1 cut(s) | 118 |
| BtgZI | GCGATG | 1 cut(s) | 111 |
| CaiI | CAGNNNCTG | 1 cut(s) | 125 |
| CciI | TCATGA | 1 cut(s) | 52 |
| Csp6I | GTAC | 1 cut(s) | 69 |
| CviAII | CATG | 1 cut(s) | 53 |
| CviJI | RGCY | 1 cut(s) | 131 |
| CviKI_1 | RGCY | 1 cut(s) | 131 |
| CviQI | GTAC | 1 cut(s) | 69 |
| DdeI | CTNAG | 1 cut(s) | 118 |
| Eam1104I | CTCTTC | 1 cut(s) | 81 |
| EarI | CTCTTC | 1 cut(s) | 81 |
| Eco57I | CTGAAG | 1 cut(s) | 147 |
| FaeI | CATG | 1 cut(s) | 56 |
| FaiI | YATR | 1 cut(s) | 54 |
| FatI | CATG | 1 cut(s) | 52 |
| FspBI | CTAG | 1 cut(s) | 150 |
| Hin1II | CATG | 1 cut(s) | 56 |
| HinfI | GANTC | 1 cut(s) | 12 |
| Hpy188III | TCNNGA | 1 cut(s) | 53 |
| HpyAV | CCTTC | 1 cut(s) | 53 |
| HpyF3I | CTNAG | 1 cut(s) | 118 |
| Hsp92II | CATG | 1 cut(s) | 56 |
| MaeI | CTAG | 1 cut(s) | 150 |
| MaeIII | GTNAC | 1 cut(s) | 121 |
| MboII | GAAGA | 2 cut(s) | 56, 98 |
| MluCI | AATT | 2 cut(s) | 160, 168 |
| MnlI | CCTC | 3 cut(s) | 82, 127, 146 |
| MroXI | GAANNNNTTC | 1 cut(s) | 160 |
| NlaIII | CATG | 1 cut(s) | 56 |
| NlaIV | GGNNCC | 1 cut(s) | 6 |
| PagI | TCATGA | 1 cut(s) | 52 |
| PdmI | GAANNNNTTC | 1 cut(s) | 160 |
| PfeI | GAWTC | 1 cut(s) | 12 |
| PspN4I | GGNNCC | 1 cut(s) | 6 |
| PstNI | CAGNNNCTG | 1 cut(s) | 125 |
| RsaI | GTAC | 1 cut(s) | 70 |
| RsaNI | GTAC | 1 cut(s) | 69 |
| SetI | ASST | 5 cut(s) | 38, 64, 70, 93, 133 |
| SgeI | CNNG | 5 cut(s) | 21, 36, 65, 77, 162 |
| Sse9I | AATT | 2 cut(s) | 160, 168 |
| SspMI | CTAG | 1 cut(s) | 150 |
| TasI | AATT | 2 cut(s) | 160, 168 |
| TfiI | GAWTC | 1 cut(s) | 12 |
| TspDTI | ATGAA | 1 cut(s) | 72 |
| XapI | RAATTY | 2 cut(s) | 160, 168 |
| XmnI | GAANNNNTTC | 1 cut(s) | 160 |
| XspI | CTAG | 1 cut(s) | 150 |