Rroxscaffold_4G00306170

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001848
Physical Location & Seq
Forward (+)
26959635 .. 26974080
14446 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00306170.1

Sequence Viewer

Length: 516 bp
ATGTCTCAAATCACCTATGGCTGCGAGACCGCAACCTCCGCCGCCACCAACCCCAACTTGGTTTTGGGTTCGTCTTTGGCTTTGGATTGGGTACTCGAATCGCTGGGCTACGTGTACGACGCTCTCTCAAATCCTTGTCTGCTCGTAGAATCTCGTCCTTTCGCTCTATCATTCATTCCAAGTCTCCTCCCGACATTCCTACACTCTCCCACTGTTTCGGTTGCTATGATCAATATTGTTGCTCCAATCTACTGGAGATTCAAAGGAGTGCCTGATGATTTCCTTGGTTTACTCAACATGAAGAATCGAAGTGGTGGCTTTGGCATTTTGCCGCTTGTCAACTCCTCGCCTTTTGAGGTCCGCCTCTTCTTCTTGCAAGAAAGTGACCGACTTCCACCCAAAGGTCGTTGTTCGTCGCCGGGTTTTGTGGAAATTGAAAACTTCCGGAGCCCAAAACCGGAATTCCTCTTCCTCCTCTGGGAGGTAGCTCGTTCGAGCCCCCACCATGGATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

171

Amino Acids

18.98

Weight (kDa)

5.88

Isoelectric Point (pI)

61.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 444
AciI CCGC 5 cut(s) 30, 39, 42, 332, 361
AcsI RAATTY 1 cut(s) 461
AfaI GTAC 2 cut(s) 93, 116
AfiI CCNNNNNNNGG 6 cut(s) 58, 401, 457, 478, 481, 506
AflIII ACRYGT 1 cut(s) 111
AgsI TTSAA 2 cut(s) 262, 437
AluBI AGCT 1 cut(s) 488
AluI AGCT 1 cut(s) 488
Alw26I GTCTC 3 cut(s) 9, 20, 188
Aor13HI TCCGGA 1 cut(s) 444
ApeKI GCWGC 1 cut(s) 21
ApoI RAATTY 1 cut(s) 461
AspS9I GGNCC 1 cut(s) 358
AsuC2I CCSGG 1 cut(s) 420
AsuHPI GGTGA 1 cut(s) 4
AvaII GGWCC 1 cut(s) 358
BanII GRGCYC 2 cut(s) 452, 500
BbvI GCAGC 1 cut(s) 8
BccI CCATC 1 cut(s) 504
BclI TGATCA 1 cut(s) 228
BcnI CCSGG 1 cut(s) 420
BcoDI GTCTC 3 cut(s) 9, 20, 188
BisI GCNGC 3 cut(s) 22, 42, 332
BlsI GCNGC 3 cut(s) 23, 43, 333
Bme1390I CCNGG 1 cut(s) 420
Bme18I GGWCC 1 cut(s) 358
BmgT120I GGNCC 1 cut(s) 358
BmiI GGNNCC 1 cut(s) 449
BmrFI CCNGG 1 cut(s) 420
BpmI CTGGAG 1 cut(s) 274
BpuMI CCSGG 1 cut(s) 420
BsaAI YACGTR 1 cut(s) 112
BsaI GGTCTC 1 cut(s) 20
BsaJI CCNNGG 2 cut(s) 283, 505
BsaWI WCCGGW 2 cut(s) 444, 457
Bsc4I CCNNNNNNNGG 6 cut(s) 58, 401, 457, 478, 481, 506
Bse1I ACTGG 1 cut(s) 257
BseAI TCCGGA 1 cut(s) 444
BseDI CCNNGG 2 cut(s) 283, 505
BseGI GGATG 1 cut(s) 515
BseLI CCNNNNNNNGG 6 cut(s) 58, 401, 457, 478, 481, 506
BseNI ACTGG 1 cut(s) 257
BseRI GAGGAG 3 cut(s) 176, 334, 464
BseXI GCAGC 1 cut(s) 8
BseYI CCCAGC 1 cut(s) 103
BsiSI CCGG 3 cut(s) 419, 445, 458
BslI CCNNNNNNNGG 6 cut(s) 58, 401, 457, 478, 481, 506
BsmAI GTCTC 3 cut(s) 9, 20, 188
Bso31I GGTCTC 1 cut(s) 20
Bsp1286I GDGCHC 2 cut(s) 452, 500
Bsp13I TCCGGA 1 cut(s) 444
Bsp143I GATC 1 cut(s) 228
Bsp19I CCATGG 1 cut(s) 505
BspACI CCGC 5 cut(s) 30, 39, 42, 332, 361
BspEI TCCGGA 1 cut(s) 444
BspLI GGNNCC 1 cut(s) 449
BspTNI GGTCTC 1 cut(s) 20
BsrI ACTGG 1 cut(s) 257
BssECI CCNNGG 2 cut(s) 283, 505
BssMI GATC 1 cut(s) 228
BssT1I CCWWGG 2 cut(s) 283, 505
Bst4CI ACNGT 1 cut(s) 214
Bst6I CTCTTC 2 cut(s) 371, 473
BstBAI YACGTR 1 cut(s) 112
BstDSI CCRYGG 1 cut(s) 505
BstENI CCTNNNNNAGG 1 cut(s) 479
BstF5I GGATG 1 cut(s) 515
BstKTI GATC 1 cut(s) 231
BstMAI GTCTC 3 cut(s) 9, 20, 188
BstMBI GATC 1 cut(s) 228
BstMWI GCNNNNNNNGC 1 cut(s) 38
BstSCI CCNGG 1 cut(s) 418
BstV1I GCAGC 1 cut(s) 8
BstXI CCANNNNNNTGG 1 cut(s) 252
BtgI CCRYGG 1 cut(s) 505
BtsCI GGATG 1 cut(s) 515
BtsIMutI CAGTG 1 cut(s) 210
Cfr13I GGNCC 1 cut(s) 358
CseI GACGC 1 cut(s) 128
Csp6I GTAC 2 cut(s) 92, 115
CviAII CATG 2 cut(s) 298, 506
CviJI RGCY 7 cut(s) 21, 80, 108, 318, 450, 488, 498
CviKI_1 RGCY 7 cut(s) 21, 80, 108, 318, 450, 488, 498
CviQI GTAC 2 cut(s) 92, 115
DpnI GATC 1 cut(s) 230
DpnII GATC 1 cut(s) 228
Eam1104I CTCTTC 2 cut(s) 371, 473
EarI CTCTTC 2 cut(s) 371, 473
EciI GGCGGA 2 cut(s) 28, 350
Eco130I CCWWGG 2 cut(s) 283, 505
Eco24I GRGCYC 2 cut(s) 452, 500
Eco31I GGTCTC 1 cut(s) 20
Eco47I GGWCC 1 cut(s) 358
EcoNI CCTNNNNNAGG 1 cut(s) 479
EcoRI GAATTC 1 cut(s) 461
EcoT14I CCWWGG 2 cut(s) 283, 505
EcoT38I GRGCYC 2 cut(s) 452, 500
ErhI CCWWGG 2 cut(s) 283, 505
FaeI CATG 2 cut(s) 301, 509
FaiI YATR 4 cut(s) 18, 227, 299, 507
FatI CATG 2 cut(s) 297, 505
FbaI TGATCA 1 cut(s) 228
Fnu4HI GCNGC 3 cut(s) 22, 42, 332
FriOI GRGCYC 2 cut(s) 452, 500
Fsp4HI GCNGC 3 cut(s) 22, 42, 332
GluI GCNGC 3 cut(s) 22, 42, 332
GsaI CCCAGC 1 cut(s) 107
GsuI CTGGAG 1 cut(s) 274
HapII CCGG 3 cut(s) 419, 445, 458
HgaI GACGC 1 cut(s) 128
Hin1II CATG 2 cut(s) 301, 509
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HinfI GANTC 4 cut(s) 98, 149, 258, 304
HpaII CCGG 3 cut(s) 419, 445, 458
HphI GGTGA 1 cut(s) 4
Hpy166II GTNNAC 3 cut(s) 115, 290, 340
Hpy188III TCNNGA 2 cut(s) 190, 445
Hpy8I GTNNAC 3 cut(s) 115, 290, 340
Hpy99I CGWCG 2 cut(s) 122, 418
HpyCH4III ACNGT 1 cut(s) 214
HpyCH4IV ACGT 1 cut(s) 111
HpyCH4V TGCA 1 cut(s) 376
HpyF10VI GCNNNNNNNGC 1 cut(s) 38
HpySE526I ACGT 1 cut(s) 111
Hsp92II CATG 2 cut(s) 301, 509
Kpn2I TCCGGA 1 cut(s) 444
Ksp22I TGATCA 1 cut(s) 228
Kzo9I GATC 1 cut(s) 228
LmnI GCTCC 2 cut(s) 247, 447
LpnPI CCDG 7 cut(s) 89, 238, 285, 432, 458, 463, 471
Lsp1109I GCAGC 1 cut(s) 8
MaeII ACGT 1 cut(s) 111
MaeIII GTNAC 1 cut(s) 383
MalI GATC 1 cut(s) 230
MboI GATC 1 cut(s) 228
MboII GAAGA 4 cut(s) 313, 358, 361, 460
MhlI GDGCHC 2 cut(s) 452, 500
MluCI AATT 2 cut(s) 432, 461
MnlI CCTC 9 cut(s) 46, 197, 349, 355, 374, 475, 476, 482, 485
MroI TCCGGA 1 cut(s) 444
MspI CCGG 3 cut(s) 419, 445, 458
MspR9I CCNGG 1 cut(s) 420
MwoI GCNNNNNNNGC 1 cut(s) 38
NciI CCSGG 1 cut(s) 420
NcoI CCATGG 1 cut(s) 505
NdeII GATC 1 cut(s) 228
NlaIII CATG 2 cut(s) 301, 509
NlaIV GGNNCC 1 cut(s) 449
NmuCI GTSAC 1 cut(s) 383
PfeI GAWTC 4 cut(s) 98, 149, 258, 304
PkrI GCNGC 3 cut(s) 23, 43, 333
Ppu21I YACGTR 1 cut(s) 112
PspFI CCCAGC 1 cut(s) 103
PspN4I GGNNCC 1 cut(s) 449
PspPI GGNCC 1 cut(s) 358
RsaI GTAC 2 cut(s) 93, 116
RsaNI GTAC 2 cut(s) 92, 115
SatI GCNGC 3 cut(s) 22, 42, 332
Sau3AI GATC 1 cut(s) 228
Sau96I GGNCC 1 cut(s) 358
ScrFI CCNGG 1 cut(s) 420
SduI GDGCHC 2 cut(s) 452, 500
SetI ASST 7 cut(s) 17, 38, 114, 360, 406, 486, 490
SinI GGWCC 1 cut(s) 358
Sse9I AATT 2 cut(s) 432, 461
SsiI CCGC 5 cut(s) 30, 39, 42, 332, 361
SspI AATATT 1 cut(s) 235
StyD4I CCNGG 1 cut(s) 418
StyI CCWWGG 2 cut(s) 283, 505
TaaI ACNGT 1 cut(s) 214
TaiI ACGT 1 cut(s) 114
TaqI TCGA 3 cut(s) 96, 307, 494
TaqII GACCGA 1 cut(s) 402
TasI AATT 2 cut(s) 432, 461
TauI GCSGC 2 cut(s) 44, 334
TfiI GAWTC 4 cut(s) 98, 149, 258, 304
TscAI CASTG 1 cut(s) 217
TseFI GTSAC 1 cut(s) 383
TseI GCWGC 1 cut(s) 21
Tsp45I GTSAC 1 cut(s) 383
TspDTI ATGAA 2 cut(s) 163, 314
TspRI CASTG 1 cut(s) 217
VpaK11BI GGWCC 1 cut(s) 358
XagI CCTNNNNNAGG 1 cut(s) 479
XapI RAATTY 1 cut(s) 461
XcmI CCANNNNNNNNNTGG 2 cut(s) 55, 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.