Rroxscaffold_5G00343110

exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001848
Physical Location & Seq
Forward (+)
12359724 .. 12366002
6279 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00343110.1

Sequence Viewer

Length: 1305 bp
ATGTTGTGTGAAGAAGGACCGAAAAGGAGCGTGAGACCGGCTCGTGAAATCAGTCCCTCGTATTCGTGTCTATCTCTTAGAAGCCAATCTTTTCTTCTCTCATCTCCCTCTCTCTTCCGCTCCCTCACTTCTCCCCCACTCCCCACCACCGATTCTCCCCACCCATCATCTTCGCTGTCTAATTCCGCCACCCTCACGCCCTCCCCGCCGTCCAATTTGAAGGGGACGAATGTTGAAAAGAATCACCTACAGCTGCGAGATCGCGACCTCCGCCGCCGCCGCCCGGTTTTGGGTTCGTCTTTGGCTTTGGATTGGGTACTCGAATTGCTGGGCTACGAGTACGACGCTCTCTCAAATCCTTTTATGGCACCGAGAATCCAATGGACAAGAACAAAAAGGTTGTTAGAAGAAAATCATGATGAAGGTCAAGAGGAACAAATTCCAAAATTCGATCAAGAAGCTGGAAACAAACATCTGAACCAGCAATCACAAAAAATAGTCAGCGTACTCAAAAGTGATGAAGTAGCAATCAATCTGGATGAGCATCGTGACAAACCAGTGAGAAATAATCAAAAAGGTCTTACTGTCATGCAAGGCCAGAGTTTGTTTGCAGATGGAAATGTTAATACATGCAATACAAATGCAGCAAGTAATGGGGAGCATCTGCTCGAAGATGAGGATATTGTTCAGATTATCAAAAAGGCTTTATCAGTTTTGAAATTGCAGAAGGGCAATCAAATGCCCGAAGATTACCCCAGATCTTTTAGAGAAGCAGCTGCAGTTTTCCAGCATGCTCAAATACGACTCGACAAGAAAATTATCAAGCATATGAGACCCATACCACAAGAGCTTGTGGAGTCCCTTGATGGAGAACTTGACTTCCTTGGATCAGAAATCCCTTCATCAATAGCCATTGCAATTGCTGAAGGAAAATTGGACCCCATAAGTATGGAGGCCTTTGATCATTTCCCTAGTTCCATTTCTAGGCATCGGTCAGATCCCATTAAGAGCCAAATTACTCATCACTTTCGAGATCCGAGATTGCTACTGTATCTGCACAAGTCAGTAGCTTTGTGGCCTTTTCCTATAAAACCAGAAAACAAGATAGATGGAACTGATGAATTAAATACTTATCTACACAAGTATCGCATAGAGTTGGATCAACATCTTGGTGCTCTTGTGGGGAGGTCAGCAGGTTTGGGTCTCTGCAGGCTCACCGGCCATTCTGTTTTCTATGACCACTTCTATGATTCGGGTGATTCCATTCATGATCAGATTGTGAAACTGGTACAGCAGTACACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03430 map03430
Pfam Domains
Protein Families
XPG_I XPG_N

Protein Analysis

434

Amino Acids

49.01

Weight (kDa)

6.42

Isoelectric Point (pI)

54.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1184
AccB1I GGYRCC 1 cut(s) 367
AccBSI CCGCTC 1 cut(s) 120
AccII CGCG 1 cut(s) 264
AciI CCGC 7 cut(s) 118, 186, 206, 271, 274, 277, 280
AclWI GGATC 4 cut(s) 895, 992, 1028, 1167
AcoI YGGCCR 1 cut(s) 1219
AcsI RAATTY 2 cut(s) 438, 446
AcuI CTGAAG 1 cut(s) 945
AfaI GTAC 5 cut(s) 318, 341, 507, 1290, 1298
AfiI CCNNNNNNNGG 4 cut(s) 283, 289, 290, 984
AgsI TTSAA 3 cut(s) 220, 236, 718
AjuI GAANNNNNNNTTGG 2 cut(s) 78, 110
AloI GAACNNNNNNTCC 2 cut(s) 864, 896
AluBI AGCT 5 cut(s) 253, 461, 776, 850, 1070
AluI AGCT 5 cut(s) 253, 461, 776, 850, 1070
Alw21I GWGCWC 1 cut(s) 1177
Alw26I GTCTC 3 cut(s) 28, 826, 1208
AlwI GGATC 4 cut(s) 895, 992, 1028, 1167
AoxI GGCC 4 cut(s) 595, 954, 1076, 1219
ApeKI GCWGC 4 cut(s) 253, 644, 773, 776
ApoI RAATTY 2 cut(s) 438, 446
Asp700I GAANNNNTTC 1 cut(s) 438
AspS9I GGNCC 2 cut(s) 17, 937
AsuC2I CCSGG 1 cut(s) 284
AsuHPI GGTGA 3 cut(s) 236, 1207, 1268
AvaII GGWCC 2 cut(s) 17, 937
BaeI ACNNNNGTAYC 3 cut(s) 1127, 1160, 1280
BanI GGYRCC 1 cut(s) 367
BauI CACGAG 1 cut(s) 42
Bbv12I GWGCWC 1 cut(s) 1177
BbvI GCAGC 4 cut(s) 240, 656, 763, 785
BccI CCATC 4 cut(s) 172, 608, 860, 1103
BceAI ACGGC 1 cut(s) 193
BclI TGATCA 2 cut(s) 961, 1270
BcnI CCSGG 1 cut(s) 284
BcoDI GTCTC 3 cut(s) 28, 826, 1208
BfaI CTAG 3 cut(s) 972, 984, 1303
BfmI CTRYAG 3 cut(s) 248, 777, 1207
BfuAI ACCTGC 1 cut(s) 1184
BglII AGATCT 1 cut(s) 758
BisI GCNGC 7 cut(s) 254, 274, 277, 280, 645, 774, 777
BlsI GCNGC 7 cut(s) 255, 275, 278, 281, 646, 775, 778
Bme1390I CCNGG 1 cut(s) 284
Bme18I GGWCC 2 cut(s) 17, 937
BmgT120I GGNCC 2 cut(s) 17, 937
BmiI GGNNCC 2 cut(s) 369, 939
BmrFI CCNGG 1 cut(s) 284
BmsI GCATC 3 cut(s) 553, 670, 997
BplI GAGNNNNNCTC 2 cut(s) 25, 57
BpuMI CCSGG 1 cut(s) 284
BsaBI GATNNNNATC 2 cut(s) 543, 1164
BsaI GGTCTC 3 cut(s) 28, 826, 1208
BsaJI CCNNGG 1 cut(s) 883
BsaXI ACNNNNNCTCC 2 cut(s) 139, 169
Bsc4I CCNNNNNNNGG 4 cut(s) 283, 289, 290, 984
Bse118I RCCGGY 2 cut(s) 37, 1217
Bse1I ACTGG 2 cut(s) 557, 1290
Bse3DI GCAATG 1 cut(s) 912
Bse8I GATNNNNATC 2 cut(s) 543, 1164
BseDI CCNNGG 1 cut(s) 883
BseGI GGATG 1 cut(s) 544
BseJI GATNNNNATC 2 cut(s) 543, 1164
BseLI CCNNNNNNNGG 4 cut(s) 283, 289, 290, 984
BseMI GCAATG 1 cut(s) 912
BseNI ACTGG 2 cut(s) 557, 1290
BseXI GCAGC 4 cut(s) 240, 656, 763, 785
BseYI CCCAGC 1 cut(s) 328
BsgI GTGCAG 1 cut(s) 1040
Bsh1236I CGCG 1 cut(s) 264
BshFI GGCC 4 cut(s) 597, 956, 1078, 1221
BshNI GGYRCC 1 cut(s) 367
BsiHKAI GWGCWC 1 cut(s) 1177
BsiSI CCGG 3 cut(s) 38, 284, 1218
BslFI GGGAC 3 cut(s) 39, 238, 844
BslI CCNNNNNNNGG 4 cut(s) 283, 289, 290, 984
BsmAI GTCTC 3 cut(s) 28, 826, 1208
BsmFI GGGAC 3 cut(s) 39, 238, 844
BsnI GGCC 4 cut(s) 597, 956, 1078, 1221
Bso31I GGTCTC 3 cut(s) 28, 826, 1208
Bsp1286I GDGCHC 1 cut(s) 1177
Bsp143I GATC 9 cut(s) 259, 451, 758, 887, 961, 997, 1033, 1159, 1270
Bsp68I TCGCGA 1 cut(s) 264
BspACI CCGC 7 cut(s) 118, 186, 206, 271, 274, 277, 280
BspANI GGCC 4 cut(s) 597, 956, 1078, 1221
BspFNI CGCG 1 cut(s) 264
BspHI TCATGA 2 cut(s) 415, 1267
BspLI GGNNCC 2 cut(s) 369, 939
BspMAI CTGCAG 2 cut(s) 781, 1211
BspMI ACCTGC 1 cut(s) 1184
BspPI GGATC 4 cut(s) 895, 992, 1028, 1167
BspT107I GGYRCC 1 cut(s) 367
BspTNI GGTCTC 3 cut(s) 28, 826, 1208
BsrBI CCGCTC 1 cut(s) 120
BsrDI GCAATG 1 cut(s) 912
BsrFI RCCGGY 2 cut(s) 37, 1217
BsrI ACTGG 2 cut(s) 557, 1290
BssAI RCCGGY 2 cut(s) 37, 1217
BssECI CCNNGG 1 cut(s) 883
BssMI GATC 9 cut(s) 259, 451, 758, 887, 961, 997, 1033, 1159, 1270
BssSI CACGAG 1 cut(s) 42
BssT1I CCWWGG 1 cut(s) 883
Bst2BI CACGAG 1 cut(s) 42
Bst4CI ACNGT 2 cut(s) 586, 1050
Bst6I CTCTTC 1 cut(s) 119
BstC8I GCNNGC 2 cut(s) 792, 1211
BstDEI CTNAG 1 cut(s) 77
BstF5I GGATG 1 cut(s) 544
BstFNI CGCG 1 cut(s) 264
BstKTI GATC 9 cut(s) 262, 454, 761, 890, 964, 1000, 1036, 1162, 1273
BstMAI GTCTC 3 cut(s) 28, 826, 1208
BstMBI GATC 9 cut(s) 259, 451, 758, 887, 961, 997, 1033, 1159, 1270
BstMWI GCNNNNNNNGC 3 cut(s) 205, 270, 279
BstNSI RCATGY 2 cut(s) 633, 794
BstSCI CCNGG 1 cut(s) 282
BstSFI CTRYAG 3 cut(s) 248, 777, 1207
BstUI CGCG 1 cut(s) 264
BstV1I GCAGC 4 cut(s) 240, 656, 763, 785
BstX2I RGATCY 3 cut(s) 758, 997, 1033
BstXI CCANNNNNNTGG 1 cut(s) 949
BstYI RGATCY 3 cut(s) 758, 997, 1033
BsuRI GGCC 4 cut(s) 597, 956, 1078, 1221
BtsCI GGATG 1 cut(s) 544
BtsIMutI CAGTG 1 cut(s) 564
BtuMI TCGCGA 1 cut(s) 264
BveI ACCTGC 1 cut(s) 1184
Cac8I GCNNGC 2 cut(s) 792, 1211
CciI TCATGA 2 cut(s) 415, 1267
Cfr10I RCCGGY 2 cut(s) 37, 1217
Cfr13I GGNCC 2 cut(s) 17, 937
CseI GACGC 1 cut(s) 353
Csp6I GTAC 5 cut(s) 317, 340, 506, 1289, 1297
CviAII CATG 5 cut(s) 416, 589, 630, 791, 1268
CviQI GTAC 5 cut(s) 317, 340, 506, 1289, 1297
DdeI CTNAG 1 cut(s) 77
DpnI GATC 9 cut(s) 261, 453, 760, 889, 963, 999, 1035, 1161, 1272
DpnII GATC 9 cut(s) 259, 451, 758, 887, 961, 997, 1033, 1159, 1270
EaeI YGGCCR 1 cut(s) 1219
Eam1104I CTCTTC 1 cut(s) 119
EarI CTCTTC 1 cut(s) 119
EciI GGCGGA 2 cut(s) 175, 260
Eco130I CCWWGG 1 cut(s) 883
Eco147I AGGCCT 1 cut(s) 956
Eco31I GGTCTC 3 cut(s) 28, 826, 1208
Eco47I GGWCC 2 cut(s) 17, 937
Eco57I CTGAAG 1 cut(s) 945
EcoT14I CCWWGG 1 cut(s) 883
ErhI CCWWGG 1 cut(s) 883
FaeI CATG 5 cut(s) 419, 592, 633, 794, 1271
FalI AAGNNNNNCTT 2 cut(s) 73, 105
FaqI GGGAC 3 cut(s) 39, 238, 844
FatI CATG 5 cut(s) 415, 588, 629, 790, 1267
FauI CCCGC 1 cut(s) 213
FauNDI CATATG 1 cut(s) 828
FbaI TGATCA 2 cut(s) 961, 1270
Fnu4HI GCNGC 7 cut(s) 254, 274, 277, 280, 645, 774, 777
FokI GGATG 1 cut(s) 551
Fsp4HI GCNGC 7 cut(s) 254, 274, 277, 280, 645, 774, 777
FspBI CTAG 3 cut(s) 972, 984, 1303
GluI GCNGC 7 cut(s) 254, 274, 277, 280, 645, 774, 777
GsaI CCCAGC 1 cut(s) 332
HaeIII GGCC 4 cut(s) 597, 956, 1078, 1221
HapII CCGG 3 cut(s) 38, 284, 1218
HgaI GACGC 1 cut(s) 353
Hin1II CATG 5 cut(s) 419, 592, 633, 794, 1271
HinfI GANTC 7 cut(s) 152, 241, 375, 804, 857, 1250, 1259
HpaII CCGG 3 cut(s) 38, 284, 1218
HphI GGTGA 3 cut(s) 236, 1207, 1268
Hpy166II GTNNAC 1 cut(s) 1299
Hpy188I TCNGA 6 cut(s) 477, 690, 892, 997, 1038, 1275
Hpy188III TCNNGA 9 cut(s) 44, 263, 416, 428, 455, 536, 548, 1031, 1268
Hpy8I GTNNAC 1 cut(s) 1299
Hpy99I CGWCG 1 cut(s) 347
HpyAV CCTTC 6 cut(s) 8, 214, 416, 721, 909, 920
HpyCH4III ACNGT 2 cut(s) 586, 1050
HpyCH4V TGCA 9 cut(s) 592, 611, 633, 644, 724, 779, 917, 1057, 1209
HpyF10VI GCNNNNNNNGC 3 cut(s) 205, 270, 279
HpyF3I CTNAG 1 cut(s) 77
Hsp92II CATG 5 cut(s) 419, 592, 633, 794, 1271
Ksp22I TGATCA 2 cut(s) 961, 1270
Kzo9I GATC 9 cut(s) 259, 451, 758, 887, 961, 997, 1033, 1159, 1270
LmnI GCTCC 3 cut(s) 27, 125, 658
Lsp1109I GCAGC 4 cut(s) 240, 656, 763, 785
LweI GCATC 3 cut(s) 553, 670, 997
MaeI CTAG 3 cut(s) 972, 984, 1303
MaeIII GTNAC 1 cut(s) 548
MalI GATC 9 cut(s) 261, 453, 760, 889, 963, 999, 1035, 1161, 1272
MbiI CCGCTC 1 cut(s) 120
MboI GATC 9 cut(s) 259, 451, 758, 887, 961, 997, 1033, 1159, 1270
MboII GAAGA 7 cut(s) 23, 86, 106, 162, 419, 683, 758
MfeI CAATTG 1 cut(s) 918
MflI RGATCY 3 cut(s) 758, 997, 1033
MhlI GDGCHC 1 cut(s) 1177
MlyI GAGTC 2 cut(s) 798, 866
MmeI TCCRAC 1 cut(s) 1137
MroXI GAANNNNTTC 1 cut(s) 438
MseI TTAA 3 cut(s) 624, 1005, 1124
MslI CAYNNNNRTG 3 cut(s) 947, 1170, 1245
MspA1I CMGCKG 2 cut(s) 253, 776
MspI CCGG 3 cut(s) 38, 284, 1218
MspR9I CCNGG 1 cut(s) 284
MunI CAATTG 1 cut(s) 918
MvnI CGCG 1 cut(s) 264
MwoI GCNNNNNNNGC 3 cut(s) 205, 270, 279
NciI CCSGG 1 cut(s) 284
NdeI CATATG 1 cut(s) 828
NdeII GATC 9 cut(s) 259, 451, 758, 887, 961, 997, 1033, 1159, 1270
NlaIII CATG 5 cut(s) 419, 592, 633, 794, 1271
NlaIV GGNNCC 2 cut(s) 369, 939
NmuCI GTSAC 1 cut(s) 548
NruI TCGCGA 1 cut(s) 264
NspI RCATGY 2 cut(s) 633, 794
PaeI GCATGC 1 cut(s) 794
PagI TCATGA 2 cut(s) 415, 1267
PceI AGGCCT 1 cut(s) 956
PdmI GAANNNNTTC 1 cut(s) 438
PfeI GAWTC 5 cut(s) 152, 241, 375, 1250, 1259
PkrI GCNGC 7 cut(s) 255, 275, 278, 281, 646, 775, 778
PleI GAGTC 2 cut(s) 798, 865
PpsI GAGTC 2 cut(s) 798, 865
PspFI CCCAGC 1 cut(s) 328
PspN4I GGNNCC 2 cut(s) 369, 939
PspPI GGNCC 2 cut(s) 17, 937
PstI CTGCAG 2 cut(s) 781, 1211
PsuI RGATCY 3 cut(s) 758, 997, 1033
PvuII CAGCTG 2 cut(s) 253, 776
RruI TCGCGA 1 cut(s) 264
RsaI GTAC 5 cut(s) 318, 341, 507, 1290, 1298
RsaNI GTAC 5 cut(s) 317, 340, 506, 1289, 1297
RseI CAYNNNNRTG 3 cut(s) 947, 1170, 1245
SaqAI TTAA 3 cut(s) 624, 1005, 1124
SatI GCNGC 7 cut(s) 254, 274, 277, 280, 645, 774, 777
Sau3AI GATC 9 cut(s) 259, 451, 758, 887, 961, 997, 1033, 1159, 1270
Sau96I GGNCC 2 cut(s) 17, 937
SchI GAGTC 2 cut(s) 798, 866
ScrFI CCNGG 1 cut(s) 284
SduI GDGCHC 1 cut(s) 1177
SfaNI GCATC 3 cut(s) 553, 670, 997
SfcI CTRYAG 3 cut(s) 248, 777, 1207
SinI GGWCC 2 cut(s) 17, 937
SmiMI CAYNNNNRTG 3 cut(s) 947, 1170, 1245
SphI GCATGC 1 cut(s) 794
SseBI AGGCCT 1 cut(s) 956
SsiI CCGC 7 cut(s) 118, 186, 206, 271, 274, 277, 280
SspMI CTAG 3 cut(s) 972, 984, 1303
StuI AGGCCT 1 cut(s) 956
StyD4I CCNGG 1 cut(s) 282
StyI CCWWGG 1 cut(s) 883
TaaI ACNGT 2 cut(s) 586, 1050
TaqI TCGA 5 cut(s) 321, 450, 669, 807, 1030
TaqII GACCGA 2 cut(s) 34, 981
TatI WGTACW 1 cut(s) 1296
TauI GCSGC 3 cut(s) 276, 279, 282
TfiI GAWTC 5 cut(s) 152, 241, 375, 1250, 1259
Tru1I TTAA 3 cut(s) 624, 1005, 1124
Tru9I TTAA 3 cut(s) 624, 1005, 1124
TscAI CASTG 1 cut(s) 564
TseFI GTSAC 1 cut(s) 548
TseI GCWGC 4 cut(s) 253, 644, 773, 776
Tsp45I GTSAC 1 cut(s) 548
TspDTI ATGAA 5 cut(s) 435, 534, 891, 1134, 1256
TspRI CASTG 1 cut(s) 564
VpaK11BI GGWCC 2 cut(s) 17, 937
XapI RAATTY 2 cut(s) 438, 446
XceI RCATGY 2 cut(s) 633, 794
XmnI GAANNNNTTC 1 cut(s) 438
XspI CTAG 3 cut(s) 972, 984, 1303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.