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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccIII | TCCGGA | 1 cut(s) | 94 |
| AclWI | GGATC | 1 cut(s) | 86 |
| AcsI | RAATTY | 1 cut(s) | 128 |
| AfaI | GTAC | 1 cut(s) | 119 |
| AluBI | AGCT | 1 cut(s) | 150 |
| AluI | AGCT | 1 cut(s) | 150 |
| Alw21I | GWGCWC | 1 cut(s) | 68 |
| AlwI | GGATC | 1 cut(s) | 86 |
| Aor13HI | TCCGGA | 1 cut(s) | 94 |
| ApeKI | GCWGC | 1 cut(s) | 78 |
| ApoI | RAATTY | 1 cut(s) | 128 |
| AspS9I | GGNCC | 1 cut(s) | 19 |
| AvaII | GGWCC | 1 cut(s) | 19 |
| Bbv12I | GWGCWC | 1 cut(s) | 68 |
| BbvI | GCAGC | 1 cut(s) | 65 |
| BccI | CCATC | 1 cut(s) | 67 |
| BceAI | ACGGC | 1 cut(s) | 91 |
| BfmI | CTRYAG | 1 cut(s) | 42 |
| BisI | GCNGC | 1 cut(s) | 79 |
| BlsI | GCNGC | 1 cut(s) | 80 |
| Bme18I | GGWCC | 1 cut(s) | 19 |
| BmgT120I | GGNCC | 1 cut(s) | 19 |
| BmiI | GGNNCC | 1 cut(s) | 20 |
| BsaWI | WCCGGW | 1 cut(s) | 94 |
| BseAI | TCCGGA | 1 cut(s) | 94 |
| BseXI | GCAGC | 1 cut(s) | 65 |
| BsiHKAI | GWGCWC | 1 cut(s) | 68 |
| BsiSI | CCGG | 1 cut(s) | 95 |
| Bsp1286I | GDGCHC | 1 cut(s) | 68 |
| Bsp13I | TCCGGA | 1 cut(s) | 94 |
| Bsp143I | GATC | 1 cut(s) | 91 |
| BspEI | TCCGGA | 1 cut(s) | 94 |
| BspLI | GGNNCC | 1 cut(s) | 20 |
| BspPI | GGATC | 1 cut(s) | 86 |
| BssMI | GATC | 1 cut(s) | 91 |
| Bst6I | CTCTTC | 1 cut(s) | 108 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 36 |
| BstKTI | GATC | 1 cut(s) | 94 |
| BstMBI | GATC | 1 cut(s) | 91 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 36 |
| BstSFI | CTRYAG | 1 cut(s) | 42 |
| BstV1I | GCAGC | 1 cut(s) | 65 |
| Cfr13I | GGNCC | 1 cut(s) | 19 |
| Csp6I | GTAC | 1 cut(s) | 118 |
| CviJI | RGCY | 2 cut(s) | 78, 150 |
| CviKI_1 | RGCY | 2 cut(s) | 78, 150 |
| CviQI | GTAC | 1 cut(s) | 118 |
| DpnI | GATC | 1 cut(s) | 93 |
| DpnII | GATC | 1 cut(s) | 91 |
| Eam1104I | CTCTTC | 1 cut(s) | 108 |
| EarI | CTCTTC | 1 cut(s) | 108 |
| Eco47I | GGWCC | 1 cut(s) | 19 |
| EcoO109I | RGGNCCY | 1 cut(s) | 19 |
| EcoRI | GAATTC | 1 cut(s) | 128 |
| FaiI | YATR | 1 cut(s) | 83 |
| Fnu4HI | GCNGC | 1 cut(s) | 79 |
| Fsp4HI | GCNGC | 1 cut(s) | 79 |
| GluI | GCNGC | 1 cut(s) | 79 |
| HapII | CCGG | 1 cut(s) | 95 |
| HpaII | CCGG | 1 cut(s) | 95 |
| Hpy188III | TCNNGA | 1 cut(s) | 95 |
| HpyCH4V | TGCA | 2 cut(s) | 30, 81 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 36 |
| Kpn2I | TCCGGA | 1 cut(s) | 94 |
| Kzo9I | GATC | 1 cut(s) | 91 |
| LpnPI | CCDG | 2 cut(s) | 16, 108 |
| Lsp1109I | GCAGC | 1 cut(s) | 65 |
| MaeIII | GTNAC | 1 cut(s) | 52 |
| MalI | GATC | 1 cut(s) | 93 |
| MboI | GATC | 1 cut(s) | 91 |
| MboII | GAAGA | 2 cut(s) | 125, 137 |
| MhlI | GDGCHC | 1 cut(s) | 68 |
| MluCI | AATT | 1 cut(s) | 128 |
| MnlI | CCTC | 3 cut(s) | 9, 115, 135 |
| MroI | TCCGGA | 1 cut(s) | 94 |
| MspI | CCGG | 1 cut(s) | 95 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 36 |
| NdeII | GATC | 1 cut(s) | 91 |
| NlaIV | GGNNCC | 1 cut(s) | 20 |
| PkrI | GCNGC | 1 cut(s) | 80 |
| PpuMI | RGGWCCY | 1 cut(s) | 19 |
| Psp5II | RGGWCCY | 1 cut(s) | 19 |
| PspN4I | GGNNCC | 1 cut(s) | 20 |
| PspPI | GGNCC | 1 cut(s) | 19 |
| PspPPI | RGGWCCY | 1 cut(s) | 19 |
| RsaI | GTAC | 1 cut(s) | 119 |
| RsaNI | GTAC | 1 cut(s) | 118 |
| SatI | GCNGC | 1 cut(s) | 79 |
| Sau3AI | GATC | 1 cut(s) | 91 |
| Sau96I | GGNCC | 1 cut(s) | 19 |
| SduI | GDGCHC | 1 cut(s) | 68 |
| SetI | ASST | 1 cut(s) | 152 |
| SfcI | CTRYAG | 1 cut(s) | 42 |
| SgeI | CNNG | 8 cut(s) | 17, 39, 43, 62, 75, 86, 107, 133 |
| SinI | GGWCC | 1 cut(s) | 19 |
| Sse9I | AATT | 1 cut(s) | 128 |
| TaqI | TCGA | 1 cut(s) | 62 |
| TasI | AATT | 1 cut(s) | 128 |
| TseI | GCWGC | 1 cut(s) | 78 |
| VpaK11BI | GGWCC | 1 cut(s) | 19 |
| XapI | RAATTY | 1 cut(s) | 128 |