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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 428 |
| AciI | CCGC | 1 cut(s) | 273 |
| AcsI | RAATTY | 3 cut(s) | 127, 303, 484 |
| AfaI | GTAC | 1 cut(s) | 374 |
| AgsI | TTSAA | 4 cut(s) | 127, 407, 459, 509 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 281 |
| ApeKI | GCWGC | 2 cut(s) | 28, 449 |
| ApoI | RAATTY | 3 cut(s) | 127, 303, 484 |
| AspS9I | GGNCC | 1 cut(s) | 352 |
| AsuHPI | GGTGA | 1 cut(s) | 488 |
| AvaII | GGWCC | 1 cut(s) | 352 |
| BbsI | GAAGAC | 1 cut(s) | 300 |
| BbvI | GCAGC | 2 cut(s) | 15, 436 |
| BccI | CCATC | 1 cut(s) | 306 |
| BisI | GCNGC | 2 cut(s) | 29, 450 |
| BlsI | GCNGC | 2 cut(s) | 30, 451 |
| Bme18I | GGWCC | 1 cut(s) | 352 |
| BmgT120I | GGNCC | 1 cut(s) | 352 |
| BmiI | GGNNCC | 3 cut(s) | 141, 271, 353 |
| BmsI | GCATC | 3 cut(s) | 335, 382, 469 |
| BpiI | GAAGAC | 1 cut(s) | 300 |
| BsaBI | GATNNNNATC | 1 cut(s) | 195 |
| Bse1I | ACTGG | 2 cut(s) | 271, 396 |
| Bse8I | GATNNNNATC | 1 cut(s) | 195 |
| BseGI | GGATG | 2 cut(s) | 307, 345 |
| BseJI | GATNNNNATC | 1 cut(s) | 195 |
| BseMII | CTCAG | 2 cut(s) | 321, 383 |
| BseNI | ACTGG | 2 cut(s) | 271, 396 |
| BseXI | GCAGC | 2 cut(s) | 15, 436 |
| BseYI | CCCAGC | 1 cut(s) | 380 |
| BsgI | GTGCAG | 1 cut(s) | 122 |
| BslFI | GGGAC | 2 cut(s) | 302, 365 |
| BsmFI | GGGAC | 2 cut(s) | 302, 365 |
| Bsp143I | GATC | 2 cut(s) | 66, 314 |
| BspACI | CCGC | 1 cut(s) | 273 |
| BspCNI | CTCAG | 2 cut(s) | 322, 382 |
| BspLI | GGNNCC | 3 cut(s) | 141, 271, 353 |
| BsrI | ACTGG | 2 cut(s) | 271, 396 |
| BssMI | GATC | 2 cut(s) | 66, 314 |
| Bst4CI | ACNGT | 3 cut(s) | 36, 98, 377 |
| BstC8I | GCNNGC | 1 cut(s) | 324 |
| BstDEI | CTNAG | 2 cut(s) | 330, 369 |
| BstF5I | GGATG | 2 cut(s) | 307, 345 |
| BstKTI | GATC | 2 cut(s) | 69, 317 |
| BstMBI | GATC | 2 cut(s) | 66, 314 |
| BstV1I | GCAGC | 2 cut(s) | 15, 436 |
| BstV2I | GAAGAC | 1 cut(s) | 300 |
| BtsCI | GGATG | 2 cut(s) | 307, 345 |
| BtsI | GCAGTG | 2 cut(s) | 444, 520 |
| BtsIMutI | CAGTG | 5 cut(s) | 32, 103, 382, 444, 520 |
| Cac8I | GCNNGC | 1 cut(s) | 324 |
| CaiI | CAGNNNCTG | 1 cut(s) | 281 |
| Cfr13I | GGNCC | 1 cut(s) | 352 |
| Csp6I | GTAC | 1 cut(s) | 373 |
| CviJI | RGCY | 1 cut(s) | 140 |
| CviKI_1 | RGCY | 1 cut(s) | 140 |
| CviQI | GTAC | 1 cut(s) | 373 |
| DdeI | CTNAG | 2 cut(s) | 330, 369 |
| DpnI | GATC | 2 cut(s) | 68, 316 |
| DpnII | GATC | 2 cut(s) | 66, 314 |
| Eco47I | GGWCC | 1 cut(s) | 352 |
| EcoRI | GAATTC | 1 cut(s) | 127 |
| FaiI | YATR | 7 cut(s) | 137, 186, 201, 257, 428, 492, 498 |
| FaqI | GGGAC | 2 cut(s) | 302, 365 |
| Fnu4HI | GCNGC | 2 cut(s) | 29, 450 |
| FokI | GGATG | 2 cut(s) | 294, 352 |
| Fsp4HI | GCNGC | 2 cut(s) | 29, 450 |
| GluI | GCNGC | 2 cut(s) | 29, 450 |
| GsaI | CCCAGC | 1 cut(s) | 384 |
| HincII | GTYRAC | 2 cut(s) | 55, 437 |
| HindII | GTYRAC | 2 cut(s) | 55, 437 |
| HpaI | GTTAAC | 1 cut(s) | 55 |
| HphI | GGTGA | 1 cut(s) | 488 |
| Hpy166II | GTNNAC | 4 cut(s) | 14, 55, 156, 437 |
| Hpy188I | TCNGA | 4 cut(s) | 85, 109, 262, 331 |
| Hpy188III | TCNNGA | 1 cut(s) | 64 |
| Hpy8I | GTNNAC | 4 cut(s) | 14, 55, 156, 437 |
| HpyCH4III | ACNGT | 3 cut(s) | 36, 98, 377 |
| HpyCH4V | TGCA | 6 cut(s) | 4, 103, 326, 452, 482, 503 |
| HpyF3I | CTNAG | 2 cut(s) | 330, 369 |
| KspAI | GTTAAC | 1 cut(s) | 55 |
| Kzo9I | GATC | 2 cut(s) | 66, 314 |
| LmnI | GCTCC | 1 cut(s) | 145 |
| LpnPI | CCDG | 7 cut(s) | 164, 251, 252, 267, 366, 409, 459 |
| Lsp1109I | GCAGC | 2 cut(s) | 15, 436 |
| LweI | GCATC | 3 cut(s) | 335, 382, 469 |
| MaeIII | GTNAC | 1 cut(s) | 174 |
| MalI | GATC | 2 cut(s) | 68, 316 |
| MboI | GATC | 2 cut(s) | 66, 314 |
| MboII | GAAGA | 2 cut(s) | 200, 305 |
| MfeI | CAATTG | 1 cut(s) | 504 |
| MluCI | AATT | 6 cut(s) | 127, 246, 303, 453, 484, 504 |
| MmeI | TCCRAC | 1 cut(s) | 71 |
| MnlI | CCTC | 2 cut(s) | 144, 330 |
| MseI | TTAA | 2 cut(s) | 54, 116 |
| MunI | CAATTG | 1 cut(s) | 504 |
| NdeII | GATC | 2 cut(s) | 66, 314 |
| NlaIV | GGNNCC | 3 cut(s) | 141, 271, 353 |
| PkrI | GCNGC | 2 cut(s) | 30, 451 |
| PsiI | TTATAA | 1 cut(s) | 428 |
| PspFI | CCCAGC | 1 cut(s) | 380 |
| PspN4I | GGNNCC | 3 cut(s) | 141, 271, 353 |
| PspPI | GGNCC | 1 cut(s) | 352 |
| PstNI | CAGNNNCTG | 1 cut(s) | 281 |
| RsaI | GTAC | 1 cut(s) | 374 |
| RsaNI | GTAC | 1 cut(s) | 373 |
| SaqAI | TTAA | 2 cut(s) | 54, 116 |
| SatI | GCNGC | 2 cut(s) | 29, 450 |
| Sau3AI | GATC | 2 cut(s) | 66, 314 |
| Sau96I | GGNCC | 1 cut(s) | 352 |
| SetI | ASST | 1 cut(s) | 155 |
| SfaNI | GCATC | 3 cut(s) | 335, 382, 469 |
| SinI | GGWCC | 1 cut(s) | 352 |
| Sse9I | AATT | 6 cut(s) | 127, 246, 303, 453, 484, 504 |
| SsiI | CCGC | 1 cut(s) | 273 |
| TaaI | ACNGT | 3 cut(s) | 36, 98, 377 |
| TaqI | TCGA | 1 cut(s) | 400 |
| TaqII | GACCGA | 1 cut(s) | 369 |
| TasI | AATT | 6 cut(s) | 127, 246, 303, 453, 484, 504 |
| TatI | WGTACW | 1 cut(s) | 372 |
| Tru1I | TTAA | 2 cut(s) | 54, 116 |
| Tru9I | TTAA | 2 cut(s) | 54, 116 |
| TscAI | CASTG | 5 cut(s) | 39, 103, 382, 451, 520 |
| TseI | GCWGC | 2 cut(s) | 28, 449 |
| TspDTI | ATGAA | 3 cut(s) | 201, 207, 296 |
| TspRI | CASTG | 5 cut(s) | 39, 103, 382, 451, 520 |
| VpaK11BI | GGWCC | 1 cut(s) | 352 |
| XapI | RAATTY | 3 cut(s) | 127, 303, 484 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 150, 245 |