Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0000396

Physical Location & Seq

Genomic Coordinates Forward (+)

28543240 .. 28546893

Gene Structure 3654 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0180500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1176 bp

ATGAACAAACAGAGACGGCCACCGCCGGACCCAGTGGCTGTGCTGAGAGGTCACCGTGCCTCTGTCACCGACCTTTGTTTCCATCCATCTCAACCTCTCTTATACACTGGTTCCGTAGACGGTGAGTTGCGGATTTGGGACACTCTGCAGAATCGGACAATAGTATCAGCAGGGGTGCATAATGCGGCACATGGGGTTGCTTCTGTTGCTTGTAGCTCTTCAATTGGAGCTGACAAAGTTATCAGCCAGGGTAGGGATGGAACTGTTAAGTGCTGGGATATTGGAGATGGAGGTCTATCCAGGGCTCCCTCTGTGATGATTGCGACAAATGCTTACCACTTCTGTAAGCTTTCGTTGGTGAAGAGACCTCATTCTTGTTCTACACAAGTTGACGGGCCAAAGCATCATGACGGAGGTGAGAGGGAGACTAGTACTACTGATGCAGATACTCTGGATGATAGTGAAGAAAAGGTCCAAGGGTATCTGTCTGAGCTGTCTAGAACCGATGAAGAAAATACTGAAGCTGGAGGATCTAAATACATTGCTATAGCAGGAGAGCAGTCTTCTGAGGTTGAGATTTGGGATCTTAATACTGCAGAAAGGTGTATGCGACTACCTCAAAGCTGTTCTGCTGACTCCTCAGCTATTTCTACCAAGGAAAGAGGAATGTGCATGGCAGTTCAAGCATTTTTACCATCAGAATCACAGGGATTCCTAAACGTCGTGGCTGGTTATGAGGATGGCTCCATGCTTTGGTGGGATATTAGAAATCCAGGTGTTCCAGTTACATCTGTCAAGTTTCATTCAGAGCCAGTTTTAAGCCTATGCATTGATGATTCATGCAATGGTGGTATCTCAGGAGCTGCAGATGAAAAAATTGTGCTCTACAGTTTGGATAATTCCATGGGTACATGTGTGATAAAAAAAGAAATTGATTTGGAGCGACCTGGCATATCAAGCACCACTATTCGACCTGATGGTAAAATATTTGCAACAGCTGGCTGGGATCACAGAGTGAGAGTATATAACTATCGCAAAGGAAATGCTTTGGCAATATTGAAGTATCACCATGCAACGTGTAATGCAGTCTCTTATTCACCAGATTGTAAGCTCATGGCCTCTGCATCAGAGGACACAGTGGTGGCACTTTGGAAACTCTATACTCCTCAAAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006810 GO:0006950 GO:0008150 GO:0009266 GO:0009314 GO:0009408 GO:0009409 GO:0009411 GO:0009416 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009628 GO:0009637 GO:0009639 GO:0009642 GO:0009644 GO:0009889 GO:0009987 GO:0010029 GO:0010030 GO:0010114 GO:0010117 GO:0010218 GO:0010224 GO:0010380 GO:0016020 GO:0019222 GO:0031323 GO:0031326 GO:0031976 GO:0031984 GO:0033554 GO:0034357 GO:0034605 GO:0034644 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0048518 GO:0048580 GO:0048582 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051171 GO:0051179 GO:0051193 GO:0051234 GO:0051239 GO:0051240 GO:0051716 GO:0055035 GO:0055085 GO:0065007 GO:0070141 GO:0071214 GO:0071478 GO:0071482 GO:0071483 GO:0071484 GO:0071486 GO:0071489 GO:0071490 GO:0071491 GO:0071492 GO:0080167 GO:0090056 GO:0104004 GO:1900140 GO:1901401 GO:1901463 GO:2000026

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Chloroa_b-bind

Protein Analysis

391

Amino Acids

42.37

Weight (kDa)

5.56

Isoelectric Point (pI)

39.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40	PF00400	11 - 47	2.4e-10	WD domain, G-beta repeat
Beta-prop_WDR5	PF25175	12 - 98	6.3e-10	WDR5 beta-propeller domain
WD40_Prp19	PF24814	12 - 97	1.3e-07	Prp19 WD40 domain
Beta-prop_WDR3_1st	PF25173	13 - 95	1.2e-11	WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	13 - 94	1.2e-07	TEP-1 second beta-propeller
Beta-prop_THOC3	PF25174	14 - 110	1.3e-08	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	188 - 308	6.9e-07	WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	220 - 385	4.4e-10	WDR3 second beta-propeller domain
WD40_Gbeta	PF25391	240 - 384	6.3e-07	G protein beta WD-40 repeat protein
WD40_Prp19	PF24814	240 - 386	1.9e-16	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	241 - 387	2.5e-16	THOC3 beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	241 - 388	5.8e-06	TEP-1 second beta-propeller
WD40_CDC20-Fz	PF24807	242 - 388	1.1e-10	CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	243 - 388	1.3e-07	WDR36/Utp21 second beta-propeller domain
WD40_MABP1-WDR62_2nd	PF24782	275 - 386	5.7e-08	MABP1/WDR62 second WD40 domain
Beta-prop_EML_2	PF23414	275 - 385	2e-07	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5	PF25175	316 - 388	5.7e-14	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	318 - 387	2e-08	WDR3 first beta-propeller domain
WD40_WDHD1_1st	PF24817	319 - 387	8e-07	WDHD1 first WD40 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000396)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G22840 AT4G14690
fragaria_vesca	FvH4_2g20400 FvH4_2g20420 FvH4_2g20430 FvH4_4g01290 FvH4_4g01310 FvH4_5g09070
malus_domestica	MD06G1134100.v1.1 MD13G1200100.v1.1 MD13G1200300.v1.1 MD13G1200600.v1.1 MD14G1150400.v1.1 MD16G1200000.v1.1
prunus_persica	Prupe.1G021400_v2.0.a1 Prupe.1G021500_v2.0.a1 Prupe.1G021600_v2.0.a1 Prupe.1G021700_v2.0.a1 Prupe.1G021800_v2.0.a1
pyrus_communis	pycom13g17350 pycom13g17370 pycom13g17400 pycom13g17410 pycom16g16870
rosa_chinensis	RchiOBHm_Chr4g0387791 RchiOBHm_Chr4g0387801 RchiOBHm_Chr6g0286501 RchiOBHm_Chr6g0286511 RchiOBHm_Chr6g0286521 RchiOBHm_Chr6g0286531 RchiOBHm_Chr7g0189141
rosa_laevigata	RLG00000004629 RLG00000010094 RLG00000010095 RLG00000012561 RLG00000012563 RLG00000012564
rosa_multiflora	Rmu_co8365229.1_g000001 Rmu_sc0004628.1_g000001 Rmu_sc0004628.1_g000002 Rmu_sc0004816.1_g000003 Rmu_sc0004816.1_g000004 Rmu_sc0004816.1_g000005 Rmu_sc0004816.1_g000010 Rmu_sc0008916.1_g000002 Rmu_sc0013958.1_g000002
rosa_roxburghii	Rroxscaffold_3G00265750 Rroxscaffold_5G00334410 Rroxscaffold_5G00334420 Rroxscaffold_7G00182230 Rroxscaffold_7G00182240 Rroxscaffold_7G00182250 Rroxscaffold_7G00182270
rosa_rugosa	Rorug03G0313400 Rorug03G0313600 Rorug06G0180400 Rorug06G0180500 Rorug06G0180600 Rorug06G0496200
rosa_samantha	Rh4AG018400 Rh4AG018500 Rh4BG013400 Rh4BG013500 Rh4CG019600 Rh4CG019700 Rh4DG014200 Rh4DG014300 Rh6AG292700 Rh6AG292800 Rh6AG292900 Rh6AG293300 Rh6AG293400 Rh6AG293500 Rh6BG296200 Rh6BG296300 Rh6BG296500 Rh6BG296600 Rh6BG296700 Rh6BG296800 Rh6BG296900 Rh6CG296800 Rh6CG297000 Rh6CG297100 Rh6CG297200 Rh6DG288700 Rh6DG288800 Rh6DG289000 Rh6DG289100 Rh7AG101000 Rh7BG103600 Rh7CG105000 Rh7CG457500 Rh7DG102900
rosa_wichuraiana	Rw4G001210 Rw6G025230 Rw6G025240 Rw6G025250 Rw6G025260 Rw6G025270 Rw6G025540 Rw7G008720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	753
AccI	GTMKAC	1 cut(s)	117
AciI	CCGC	3 cut(s)	23, 130, 185
AclWI	GGATC	3 cut(s)	538, 591, 1014
AcoI	YGGCCR	1 cut(s)	17
AcuI	CTGAAG	1 cut(s)	540
AdeI	CACNNNGTG	1 cut(s)	1015
AfaI	GTAC	2 cut(s)	433, 910
AfiI	CCNNNNNNNGG	2 cut(s)	253, 753
AflIII	ACRYGT	2 cut(s)	911, 1076
AgsI	TTSAA	3 cut(s)	222, 683, 1060
AhlI	ACTAGT	1 cut(s)	428
AjnI	CCWGG	4 cut(s)	246, 299, 772, 946
AleI	CACNNNNGTG	1 cut(s)	1139
Alw21I	GWGCWC	1 cut(s)	885
Alw26I	GTCTC	4 cut(s)	7, 358, 419, 1093
AlwI	GGATC	3 cut(s)	538, 591, 1014
AlwNI	CAGNNNCTG	2 cut(s)	38, 863
AoxI	GGCC	3 cut(s)	17, 395, 1116
ApeKI	GCWGC	1 cut(s)	863
AspS9I	GGNCC	3 cut(s)	28, 395, 472
AsuHPI	GGTGA	7 cut(s)	44, 58, 134, 370, 428, 1058, 1089
AvaII	GGWCC	2 cut(s)	28, 472
BaeI	ACNNNNGTAYC	4 cut(s)	147, 180, 900, 933
BanII	GRGCYC	1 cut(s)	307
BbsI	GAAGAC	1 cut(s)	555
Bbv12I	GWGCWC	1 cut(s)	885
BbvCI	CCTCAGC	1 cut(s)	640
BbvI	GCAGC	1 cut(s)	850
BccI	CCATC	7 cut(s)	90, 94, 251, 281, 703, 734, 971
BceAI	ACGGC	1 cut(s)	32
BciT130I	CCWGG	4 cut(s)	248, 301, 774, 948
BcoDI	GTCTC	4 cut(s)	7, 358, 419, 1093
BcuI	ACTAGT	1 cut(s)	428
BfaI	CTAG	2 cut(s)	429, 498
BfmI	CTRYAG	5 cut(s)	146, 546, 594, 864, 886
BisI	GCNGC	2 cut(s)	186, 864
BlsI	GCNGC	2 cut(s)	187, 865
BmcAI	AGTACT	1 cut(s)	433
Bme1390I	CCNGG	4 cut(s)	248, 301, 774, 948
Bme18I	GGWCC	2 cut(s)	28, 472
BmgT120I	GGNCC	3 cut(s)	28, 395, 472
BmiI	GGNNCC	4 cut(s)	30, 112, 306, 745
BmrFI	CCNGG	4 cut(s)	248, 301, 774, 948
BmrI	ACTGGG	1 cut(s)	26
BmsI	GCATC	3 cut(s)	412, 430, 1133
BmuI	ACTGGG	1 cut(s)	26
BpiI	GAAGAC	1 cut(s)	555
BplI	GAGNNNNNCTC	2 cut(s)	728, 760
BpmI	CTGGAG	1 cut(s)	546
Bpu10I	CCTNAGC	1 cut(s)	640
BsaI	GGTCTC	1 cut(s)	358
BsaJI	CCNNGG	5 cut(s)	247, 300, 475, 654, 903
Bsc4I	CCNNNNNNNGG	2 cut(s)	253, 753
Bse1I	ACTGG	4 cut(s)	32, 112, 782, 812
Bse3DI	GCAATG	2 cut(s)	540, 850
BseBI	CCWGG	4 cut(s)	248, 301, 774, 948
BseDI	CCNNGG	5 cut(s)	247, 300, 475, 654, 903
BseGI	GGATG	4 cut(s)	82, 262, 460, 745
BseLI	CCNNNNNNNGG	2 cut(s)	253, 753
BseMI	GCAATG	2 cut(s)	540, 850
BseMII	CTCAG	5 cut(s)	35, 480, 558, 654, 870
BseNI	ACTGG	4 cut(s)	32, 112, 782, 812
BseRI	GAGGAG	2 cut(s)	628, 1155
BseXI	GCAGC	1 cut(s)	850
BseYI	CCCAGC	2 cut(s)	273, 1002
BshFI	GGCC	3 cut(s)	19, 397, 1118
BsiHKAI	GWGCWC	1 cut(s)	885
BsiSI	CCGG	1 cut(s)	26
BslFI	GGGAC	1 cut(s)	152
BslI	CCNNNNNNNGG	2 cut(s)	253, 753
BsmAI	GTCTC	4 cut(s)	7, 358, 419, 1093
BsmBI	CGTCTC	1 cut(s)	7
BsmFI	GGGAC	1 cut(s)	152
BsnI	GGCC	3 cut(s)	19, 397, 1118
Bso31I	GGTCTC	1 cut(s)	358
Bsp1286I	GDGCHC	2 cut(s)	307, 885
Bsp143I	GATC	3 cut(s)	530, 583, 1006
Bsp19I	CCATGG	1 cut(s)	903
BspACI	CCGC	3 cut(s)	23, 130, 185
BspANI	GGCC	3 cut(s)	19, 397, 1118
BspCNI	CTCAG	5 cut(s)	36, 481, 559, 653, 869
BspHI	TCATGA	1 cut(s)	406
BspLI	GGNNCC	4 cut(s)	30, 112, 306, 745
BspMAI	CTGCAG	3 cut(s)	150, 598, 868
BspPI	GGATC	3 cut(s)	538, 591, 1014
BspQI	GCTCTTC	1 cut(s)	223
BspTNI	GGTCTC	1 cut(s)	358
BsrDI	GCAATG	2 cut(s)	540, 850
BsrI	ACTGG	4 cut(s)	32, 112, 782, 812
BssECI	CCNNGG	5 cut(s)	247, 300, 475, 654, 903
BssMI	GATC	3 cut(s)	530, 583, 1006
BssT1I	CCWWGG	3 cut(s)	475, 654, 903
Bst2UI	CCWGG	4 cut(s)	248, 301, 774, 948
Bst4CI	ACNGT	5 cut(s)	56, 122, 265, 890, 1138
Bst6I	CTCTTC	2 cut(s)	223, 356
BstC8I	GCNNGC	1 cut(s)	1000
BstDEI	CTNAG	5 cut(s)	44, 489, 567, 640, 856
BstDSI	CCRYGG	1 cut(s)	903
BstEII	GGTNACC	1 cut(s)	50
BstF5I	GGATG	4 cut(s)	82, 262, 460, 745
BstKTI	GATC	3 cut(s)	533, 586, 1009
BstMAI	GTCTC	4 cut(s)	7, 358, 419, 1093
BstMBI	GATC	3 cut(s)	530, 583, 1006
BstMWI	GCNNNNNNNGC	4 cut(s)	206, 329, 683, 957
BstNI	CCWGG	4 cut(s)	248, 301, 774, 948
BstNSI	RCATGY	1 cut(s)	915
BstPI	GGTNACC	1 cut(s)	50
BstSCI	CCNGG	4 cut(s)	246, 299, 772, 946
BstSFI	CTRYAG	5 cut(s)	146, 546, 594, 864, 886
BstV1I	GCAGC	1 cut(s)	850
BstV2I	GAAGAC	1 cut(s)	555
BstX2I	RGATCY	2 cut(s)	530, 583
BstYI	RGATCY	2 cut(s)	530, 583
BsuRI	GGCC	3 cut(s)	19, 397, 1118
BtgI	CCRYGG	1 cut(s)	903
BtsCI	GGATG	4 cut(s)	82, 262, 460, 745
BtsIMutI	CAGTG	3 cut(s)	39, 105, 1143
Cac8I	GCNNGC	1 cut(s)	1000
CaiI	CAGNNNCTG	2 cut(s)	38, 863
CciI	TCATGA	1 cut(s)	406
Cfr13I	GGNCC	3 cut(s)	28, 395, 472
Csp6I	GTAC	2 cut(s)	432, 909
CviAII	CATG	9 cut(s)	191, 407, 673, 748, 840, 904, 912, 1070, 1114
CviQI	GTAC	2 cut(s)	432, 909
DdeI	CTNAG	5 cut(s)	44, 489, 567, 640, 856
DpnI	GATC	3 cut(s)	532, 585, 1008
DpnII	GATC	3 cut(s)	530, 583, 1006
DraIII	CACNNNGTG	1 cut(s)	1015
EaeI	YGGCCR	1 cut(s)	17
Eam1104I	CTCTTC	2 cut(s)	223, 356
EarI	CTCTTC	2 cut(s)	223, 356
Eco130I	CCWWGG	3 cut(s)	475, 654, 903
Eco24I	GRGCYC	1 cut(s)	307
Eco31I	GGTCTC	1 cut(s)	358
Eco47I	GGWCC	2 cut(s)	28, 472
Eco57I	CTGAAG	1 cut(s)	540
Eco91I	GGTNACC	1 cut(s)	50
EcoO65I	GGTNACC	1 cut(s)	50
EcoRII	CCWGG	4 cut(s)	246, 299, 772, 946
EcoT14I	CCWWGG	3 cut(s)	475, 654, 903
EcoT22I	ATGCAT	1 cut(s)	830
EcoT38I	GRGCYC	1 cut(s)	307
ErhI	CCWWGG	3 cut(s)	475, 654, 903
Esp3I	CGTCTC	1 cut(s)	7
FaeI	CATG	9 cut(s)	194, 410, 676, 751, 843, 907, 915, 1073, 1117
FaqI	GGGAC	1 cut(s)	152
FatI	CATG	9 cut(s)	190, 406, 672, 747, 839, 903, 911, 1069, 1113
FblI	GTMKAC	1 cut(s)	117
Fnu4HI	GCNGC	2 cut(s)	186, 864
FokI	GGATG	4 cut(s)	69, 269, 467, 752
FriOI	GRGCYC	1 cut(s)	307
Fsp4HI	GCNGC	2 cut(s)	186, 864
FspBI	CTAG	2 cut(s)	429, 498
GluI	GCNGC	2 cut(s)	186, 864
GsaI	CCCAGC	2 cut(s)	277, 1006
GsuI	CTGGAG	1 cut(s)	546
HaeIII	GGCC	3 cut(s)	19, 397, 1118
HapII	CCGG	1 cut(s)	26
Hin1II	CATG	9 cut(s)	194, 410, 676, 751, 843, 907, 915, 1073, 1117
HincII	GTYRAC	1 cut(s)	391
HindII	GTYRAC	1 cut(s)	391
HindIII	AAGCTT	1 cut(s)	347
HinfI	GANTC	5 cut(s)	151, 635, 701, 711, 836
HpaII	CCGG	1 cut(s)	26
HphI	GGTGA	7 cut(s)	44, 58, 134, 370, 428, 1058, 1089
Hpy166II	GTNNAC	2 cut(s)	118, 391
Hpy188I	TCNGA	6 cut(s)	156, 490, 568, 700, 808, 1129
Hpy188III	TCNNGA	4 cut(s)	407, 452, 498, 858
Hpy8I	GTNNAC	2 cut(s)	118, 391
Hpy99I	CGWCG	1 cut(s)	725
HpyCH4III	ACNGT	5 cut(s)	56, 122, 265, 890, 1138
HpyCH4IV	ACGT	2 cut(s)	720, 1076
HpyF10VI	GCNNNNNNNGC	4 cut(s)	206, 329, 683, 957
HpyF3I	CTNAG	5 cut(s)	44, 489, 567, 640, 856
HpySE526I	ACGT	2 cut(s)	720, 1076
Hsp92II	CATG	9 cut(s)	194, 410, 676, 751, 843, 907, 915, 1073, 1117
Kzo9I	GATC	3 cut(s)	530, 583, 1006
LguI	GCTCTTC	1 cut(s)	223
LmnI	GCTCC	5 cut(s)	227, 310, 749, 860, 940
Lsp1109I	GCAGC	1 cut(s)	850
LweI	GCATC	3 cut(s)	412, 430, 1133
MaeI	CTAG	2 cut(s)	429, 498
MaeII	ACGT	2 cut(s)	720, 1076
MaeIII	GTNAC	3 cut(s)	50, 64, 784
MalI	GATC	3 cut(s)	532, 585, 1008
MboI	GATC	3 cut(s)	530, 583, 1006
MboII	GAAGA	5 cut(s)	210, 373, 476, 521, 555
MfeI	CAATTG	1 cut(s)	222
MflI	RGATCY	2 cut(s)	530, 583
MhlI	GDGCHC	2 cut(s)	307, 885
MluCI	AATT	4 cut(s)	222, 876, 898, 930
MlyI	GAGTC	1 cut(s)	629
Mph1103I	ATGCAT	1 cut(s)	830
MseI	TTAA	3 cut(s)	267, 588, 818
MslI	CAYNNNNRTG	1 cut(s)	1139
MspA1I	CMGCKG	1 cut(s)	998
MspI	CCGG	1 cut(s)	26
MspR9I	CCNGG	4 cut(s)	248, 301, 774, 948
MunI	CAATTG	1 cut(s)	222
MvaI	CCWGG	4 cut(s)	248, 301, 774, 948
MwoI	GCNNNNNNNGC	4 cut(s)	206, 329, 683, 957
NcoI	CCATGG	1 cut(s)	903
NdeII	GATC	3 cut(s)	530, 583, 1006
NlaIII	CATG	9 cut(s)	194, 410, 676, 751, 843, 907, 915, 1073, 1117
NlaIV	GGNNCC	4 cut(s)	30, 112, 306, 745
NmuCI	GTSAC	2 cut(s)	50, 64
NsiI	ATGCAT	1 cut(s)	830
NspI	RCATGY	1 cut(s)	915
OliI	CACNNNNGTG	1 cut(s)	1139
PagI	TCATGA	1 cut(s)	406
PciI	ACATGT	1 cut(s)	911
PciSI	GCTCTTC	1 cut(s)	223
PfeI	GAWTC	4 cut(s)	151, 701, 711, 836
PflMI	CCANNNNNTGG	1 cut(s)	753
PkrI	GCNGC	2 cut(s)	187, 865
PleI	GAGTC	1 cut(s)	629
PpsI	GAGTC	1 cut(s)	629
PscI	ACATGT	1 cut(s)	911
Psp6I	CCWGG	4 cut(s)	246, 299, 772, 946
PspEI	GGTNACC	1 cut(s)	50
PspFI	CCCAGC	2 cut(s)	273, 1002
PspGI	CCWGG	4 cut(s)	246, 299, 772, 946
PspN4I	GGNNCC	4 cut(s)	30, 112, 306, 745
PspPI	GGNCC	3 cut(s)	28, 395, 472
PstI	CTGCAG	3 cut(s)	150, 598, 868
PstNI	CAGNNNCTG	2 cut(s)	38, 863
PsuI	RGATCY	2 cut(s)	530, 583
PvuII	CAGCTG	1 cut(s)	998
RsaI	GTAC	2 cut(s)	433, 910
RsaNI	GTAC	2 cut(s)	432, 909
RseI	CAYNNNNRTG	1 cut(s)	1139
SapI	GCTCTTC	1 cut(s)	223
SaqAI	TTAA	3 cut(s)	267, 588, 818
SatI	GCNGC	2 cut(s)	186, 864
Sau3AI	GATC	3 cut(s)	530, 583, 1006
Sau96I	GGNCC	3 cut(s)	28, 395, 472
ScaI	AGTACT	1 cut(s)	433
SchI	GAGTC	1 cut(s)	629
ScrFI	CCNGG	4 cut(s)	248, 301, 774, 948
SduI	GDGCHC	2 cut(s)	307, 885
SfaNI	GCATC	3 cut(s)	412, 430, 1133
SfcI	CTRYAG	5 cut(s)	146, 546, 594, 864, 886
SinI	GGWCC	2 cut(s)	28, 472
SmiMI	CAYNNNNRTG	1 cut(s)	1139
SpeI	ACTAGT	1 cut(s)	428
Sse9I	AATT	4 cut(s)	222, 876, 898, 930
SsiI	CCGC	3 cut(s)	23, 130, 185
SspI	AATATT	2 cut(s)	987, 1056
SspMI	CTAG	2 cut(s)	429, 498
StyD4I	CCNGG	4 cut(s)	246, 299, 772, 946
StyI	CCWWGG	3 cut(s)	475, 654, 903
TaaI	ACNGT	5 cut(s)	56, 122, 265, 890, 1138
TaiI	ACGT	2 cut(s)	723, 1079
TaqI	TCGA	1 cut(s)	970
TasI	AATT	4 cut(s)	222, 876, 898, 930
TatI	WGTACW	1 cut(s)	431
TauI	GCSGC	1 cut(s)	188
TfiI	GAWTC	4 cut(s)	151, 701, 711, 836
Tru1I	TTAA	3 cut(s)	267, 588, 818
Tru9I	TTAA	3 cut(s)	267, 588, 818
TscAI	CASTG	3 cut(s)	39, 112, 1143
TseFI	GTSAC	2 cut(s)	50, 64
TseI	GCWGC	1 cut(s)	863
Tsp45I	GTSAC	2 cut(s)	50, 64
TspDTI	ATGAA	5 cut(s)	17, 522, 791, 828, 885
TspGWI	ACGGA	2 cut(s)	103, 426
TspRI	CASTG	3 cut(s)	39, 112, 1143
Van91I	CCANNNNNTGG	1 cut(s)	753
VpaK11BI	GGWCC	2 cut(s)	28, 472
XbaI	TCTAGA	1 cut(s)	497
XceI	RCATGY	1 cut(s)	915
XcmI	CCANNNNNNNNNTGG	1 cut(s)	254
XmiI	GTMKAC	1 cut(s)	117
XspI	CTAG	2 cut(s)	429, 498
ZrmI	AGTACT	1 cut(s)	433
Zsp2I	ATGCAT	1 cut(s)	830

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.