Rh5DG539300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000459
Physical Location & Seq
Forward (+)
83101815 .. 83105742
3928 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG539300.1

Sequence Viewer

Length: 408 bp
ATGAGCAGCCGCTTGTGGAGGAGCACTACTCCAATAGTGGAGAAATATGATGGTGATGGTGGAGGATTGATGCCAGCTATTTTGGATACAGGGACGACGAGGATGGGTTTTGGAGAAGCTGGAGCTGTTCTACATTTCAAGAATATGGCTCTTCGTTCTGTGCTTGGAAAGATGGGGAAAACTATGACTATGACGAGTAGCAAATGTATTCACAAAAATGGAAGACTAGTTTTGAAGCTTGGGAATTTTGAGGCCGAAGCTATCAACCACAAAAACAATAACAATAATAAGGATGCGCCTTCGATGGATGATCCTCTTACTGCTTATCTCTCTGAAATGGGTTGTTATGATGAATATGGGATTCTAATTCCAATGACTGTTAATCGGGTATATGATGTTAGTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

135

Amino Acids

14.86

Weight (kDa)

7.77

Isoelectric Point (pI)

40.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000459)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G01590 AT4G01590 AT4G01590 AT4G35685
fragaria_vesca FvH4_2g26230 FvH4_3g43010 FvH4_3g43011 FvH4_4g18161 FvH4_4g18202 FvH4_5g23500 FvH4_6g36040 FvH4_6g36040 FvH4_6g36040
malus_domestica MD08G1165000.v1.1 MD08G1171800.v1.1 MD15G1357300.v1.1 MD15G1382800.v1.1
prunus_persica Prupe.1G504500_v2.0.a1 Prupe.1G504500_v2.0.a1 Prupe.1G504500_v2.0.a1 Prupe.1G504500_v2.0.a1 Prupe.1G504500_v2.0.a1 Prupe.1G504500_v2.0.a1 Prupe.1G582300_v2.0.a1 Prupe.6G034400_v2.0.a1 Prupe.6G034600_v2.0.a1
pyrus_communis pycom02g25390 pycom08g14650
rosa_chinensis RchiOBHm_Chr2g0143271 RchiOBHm_Chr4g0422341 RchiOBHm_Chr6g0253151 RchiOBHm_Chr6g0294801
rosa_laevigata RLG00000007621 RLG00000011839 RLG00000020015 RLG00000020019 RLG00000020023 RLG00000036652 RLG00000036658
rosa_multiflora Rmu_co8140216.1_g000001 Rmu_co8510555.1_g000002 Rmu_sc0003323.1_g000013 Rmu_sc0003323.1_g000024 Rmu_sc0003323.1_g000031 Rmu_sc0013245.1_g000016 Rmu_sc0030690.1_g000001
rosa_roxburghii Rroxscaffold_1G00005030 Rroxscaffold_2G00101900 Rroxscaffold_2G00102030 Rroxscaffold_4G00318680 Rroxscaffold_5G00364400 Rroxscaffold_5G00364410 Rroxscaffold_7G00173000 Rroxscaffold_7G00212110
rosa_rugosa Rorug04G0179200 Rorug05G0451000 Rorug05G0451400.1 Rorug05G0451500 Rorug06G0246900
rosa_samantha Rh1AG104900 Rh1CG244000 Rh1DG126500 Rh2AG432700 Rh2CG418500 Rh4AG238500 Rh4BG241700 Rh4BG242000 Rh4CG253500 Rh4CG253600 Rh4DG237400 Rh5AG505300 Rh5AG505500 Rh5BG527000 Rh5BG527200 Rh5BG527500 Rh5CG551000 Rh5CG551300 Rh5CG551800 Rh5DG539300 Rh5DG539900 Rh6AG057300 Rh6AG360500 Rh6BG051900 Rh6BG367200 Rh6CG049800 Rh6CG374200 Rh6DG046000
rosa_wichuraiana Rw1G009810 Rw5G047080 Rw5G047100 Rw6G031450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 10
AclWI GGATC 1 cut(s) 305
AcsI RAATTY 1 cut(s) 244
AgsI TTSAA 2 cut(s) 139, 235
AhlI ACTAGT 1 cut(s) 226
AluBI AGCT 5 cut(s) 77, 119, 125, 238, 260
AluI AGCT 5 cut(s) 77, 119, 125, 238, 260
Alw21I GWGCWC 1 cut(s) 26
AlwI GGATC 1 cut(s) 305
AoxI GGCC 1 cut(s) 252
ApeKI GCWGC 1 cut(s) 6
ApoI RAATTY 1 cut(s) 244
AspLEI GCGC 1 cut(s) 298
AsuHPI GGTGA 1 cut(s) 65
BbsI GAAGAC 1 cut(s) 229
Bbv12I GWGCWC 1 cut(s) 26
BbvI GCAGC 1 cut(s) 18
BccI CCATC 5 cut(s) 44, 50, 97, 166, 298
BciVI GTATCC 1 cut(s) 79
BcuI ACTAGT 1 cut(s) 226
BfaI CTAG 1 cut(s) 227
BfuI GTATCC 1 cut(s) 79
BisI GCNGC 2 cut(s) 7, 10
BlsI GCNGC 2 cut(s) 8, 11
BmsI GCATC 2 cut(s) 60, 283
BpiI GAAGAC 1 cut(s) 229
BplI GAGNNNNNCTC 2 cut(s) 13, 45
BpmI CTGGAG 1 cut(s) 141
BseGI GGATG 3 cut(s) 108, 298, 313
BseRI GAGGAG 1 cut(s) 34
BseXI GCAGC 1 cut(s) 18
BshFI GGCC 1 cut(s) 254
BsiHKAI GWGCWC 1 cut(s) 26
BslFI GGGAC 1 cut(s) 106
BsmFI GGGAC 1 cut(s) 106
BsnI GGCC 1 cut(s) 254
Bsp1286I GDGCHC 1 cut(s) 26
Bsp143I GATC 1 cut(s) 310
BspACI CCGC 1 cut(s) 10
BspANI GGCC 1 cut(s) 254
BspPI GGATC 1 cut(s) 305
BspQI GCTCTTC 1 cut(s) 156
BssMI GATC 1 cut(s) 310
Bst4CI ACNGT 1 cut(s) 379
Bst6I CTCTTC 1 cut(s) 156
BstC8I GCNNGC 1 cut(s) 75
BstF5I GGATG 3 cut(s) 108, 298, 313
BstHHI GCGC 1 cut(s) 298
BstKTI GATC 1 cut(s) 313
BstMBI GATC 1 cut(s) 310
BstV1I GCAGC 1 cut(s) 18
BstV2I GAAGAC 1 cut(s) 229
BsuI GTATCC 1 cut(s) 79
BsuRI GGCC 1 cut(s) 254
BtsCI GGATG 3 cut(s) 108, 298, 313
Cac8I GCNNGC 1 cut(s) 75
CfoI GCGC 1 cut(s) 298
CviJI RGCY 8 cut(s) 9, 77, 119, 125, 149, 238, 254, 260
CviKI_1 RGCY 8 cut(s) 9, 77, 119, 125, 149, 238, 254, 260
DpnI GATC 1 cut(s) 312
DpnII GATC 1 cut(s) 310
Eam1104I CTCTTC 1 cut(s) 156
EarI CTCTTC 1 cut(s) 156
FaiI YATR 8 cut(s) 48, 146, 185, 191, 348, 357, 391, 393
FaqI GGGAC 1 cut(s) 106
Fnu4HI GCNGC 2 cut(s) 7, 10
FokI GGATG 3 cut(s) 115, 305, 320
Fsp4HI GCNGC 2 cut(s) 7, 10
FspBI CTAG 1 cut(s) 227
GlaI GCGC 1 cut(s) 297
GluI GCNGC 2 cut(s) 7, 10
GsuI CTGGAG 1 cut(s) 141
HaeIII GGCC 1 cut(s) 254
HhaI GCGC 1 cut(s) 298
Hin6I GCGC 1 cut(s) 296
HinP1I GCGC 1 cut(s) 296
HindIII AAGCTT 1 cut(s) 236
HinfI GANTC 1 cut(s) 361
HphI GGTGA 1 cut(s) 65
Hpy188I TCNGA 1 cut(s) 334
Hpy188III TCNNGA 1 cut(s) 139
Hpy99I CGWCG 1 cut(s) 100
HpyAV CCTTC 1 cut(s) 309
HpyCH4III ACNGT 1 cut(s) 379
HspAI GCGC 1 cut(s) 296
Kzo9I GATC 1 cut(s) 310
LguI GCTCTTC 1 cut(s) 156
LmnI GCTCC 2 cut(s) 21, 122
LpnPI CCDG 3 cut(s) 75, 87, 105
Lsp1109I GCAGC 1 cut(s) 18
LweI GCATC 2 cut(s) 60, 283
MaeI CTAG 1 cut(s) 227
MaeIII GTNAC 1 cut(s) 401
MalI GATC 1 cut(s) 312
MboI GATC 1 cut(s) 310
MboII GAAGA 2 cut(s) 143, 234
MhlI GDGCHC 1 cut(s) 26
MluCI AATT 2 cut(s) 244, 366
MnlI CCTC 5 cut(s) 12, 56, 93, 244, 324
MseI TTAA 1 cut(s) 381
MslI CAYNNNNRTG 1 cut(s) 216
NdeII GATC 1 cut(s) 310
PciSI GCTCTTC 1 cut(s) 156
PfeI GAWTC 1 cut(s) 361
PkrI GCNGC 2 cut(s) 8, 11
RseI CAYNNNNRTG 1 cut(s) 216
SapI GCTCTTC 1 cut(s) 156
SaqAI TTAA 1 cut(s) 381
SatI GCNGC 2 cut(s) 7, 10
Sau3AI GATC 1 cut(s) 310
SduI GDGCHC 1 cut(s) 26
SetI ASST 5 cut(s) 79, 121, 127, 240, 262
SfaNI GCATC 2 cut(s) 60, 283
SmiMI CAYNNNNRTG 1 cut(s) 216
SpeI ACTAGT 1 cut(s) 226
Sse9I AATT 2 cut(s) 244, 366
SsiI CCGC 1 cut(s) 10
SspMI CTAG 1 cut(s) 227
TaaI ACNGT 1 cut(s) 379
TaqI TCGA 1 cut(s) 302
TasI AATT 2 cut(s) 244, 366
TauI GCSGC 1 cut(s) 12
TfiI GAWTC 1 cut(s) 361
Tru1I TTAA 1 cut(s) 381
Tru9I TTAA 1 cut(s) 381
TseI GCWGC 1 cut(s) 6
TspDTI ATGAA 1 cut(s) 366
XapI RAATTY 1 cut(s) 244
XspI CTAG 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.