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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 39 |
| AclWI | GGATC | 1 cut(s) | 274 |
| AcsI | RAATTY | 1 cut(s) | 62 |
| AcyI | GRCGYC | 1 cut(s) | 69 |
| AfaI | GTAC | 2 cut(s) | 171, 225 |
| AgsI | TTSAA | 2 cut(s) | 158, 192 |
| AleI | CACNNNNGTG | 1 cut(s) | 24 |
| AluBI | AGCT | 3 cut(s) | 43, 113, 275 |
| AluI | AGCT | 3 cut(s) | 43, 113, 275 |
| AlwI | GGATC | 1 cut(s) | 274 |
| ApeKI | GCWGC | 1 cut(s) | 272 |
| ApoI | RAATTY | 1 cut(s) | 62 |
| BbvI | GCAGC | 1 cut(s) | 284 |
| BclI | TGATCA | 1 cut(s) | 28 |
| BfaI | CTAG | 1 cut(s) | 114 |
| BisI | GCNGC | 1 cut(s) | 273 |
| BlsI | GCNGC | 1 cut(s) | 274 |
| BmsI | GCATC | 1 cut(s) | 124 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 240, 272 |
| BpuEI | CTTGAG | 1 cut(s) | 227 |
| BsaHI | GRCGYC | 1 cut(s) | 69 |
| Bse1I | ACTGG | 1 cut(s) | 313 |
| Bse3DI | GCAATG | 1 cut(s) | 55 |
| BseMI | GCAATG | 1 cut(s) | 55 |
| BseNI | ACTGG | 1 cut(s) | 313 |
| BseXI | GCAGC | 1 cut(s) | 284 |
| BsmI | GAATGC | 1 cut(s) | 212 |
| Bsp143I | GATC | 2 cut(s) | 28, 279 |
| BspPI | GGATC | 1 cut(s) | 274 |
| BsrDI | GCAATG | 1 cut(s) | 55 |
| BsrI | ACTGG | 1 cut(s) | 313 |
| BssMI | GATC | 2 cut(s) | 28, 279 |
| BssNI | GRCGYC | 1 cut(s) | 69 |
| Bst4CI | ACNGT | 1 cut(s) | 178 |
| BstACI | GRCGYC | 1 cut(s) | 69 |
| BstC8I | GCNNGC | 2 cut(s) | 17, 249 |
| BstDEI | CTNAG | 1 cut(s) | 138 |
| BstKTI | GATC | 2 cut(s) | 31, 282 |
| BstMBI | GATC | 2 cut(s) | 28, 279 |
| BstV1I | GCAGC | 1 cut(s) | 284 |
| Cac8I | GCNNGC | 2 cut(s) | 17, 249 |
| CseI | GACGC | 1 cut(s) | 77 |
| Csp6I | GTAC | 2 cut(s) | 170, 224 |
| CviJI | RGCY | 4 cut(s) | 43, 113, 151, 275 |
| CviKI_1 | RGCY | 4 cut(s) | 43, 113, 151, 275 |
| CviQI | GTAC | 2 cut(s) | 170, 224 |
| DdeI | CTNAG | 1 cut(s) | 138 |
| DpnI | GATC | 2 cut(s) | 30, 281 |
| DpnII | GATC | 2 cut(s) | 28, 279 |
| FaiI | YATR | 3 cut(s) | 39, 231, 278 |
| FbaI | TGATCA | 1 cut(s) | 28 |
| Fnu4HI | GCNGC | 1 cut(s) | 273 |
| Fsp4HI | GCNGC | 1 cut(s) | 273 |
| FspBI | CTAG | 1 cut(s) | 114 |
| GluI | GCNGC | 1 cut(s) | 273 |
| HgaI | GACGC | 1 cut(s) | 77 |
| Hin1I | GRCGYC | 1 cut(s) | 69 |
| HincII | GTYRAC | 1 cut(s) | 181 |
| HindII | GTYRAC | 1 cut(s) | 181 |
| HindIII | AAGCTT | 1 cut(s) | 41 |
| HinfI | GANTC | 1 cut(s) | 158 |
| Hpy166II | GTNNAC | 1 cut(s) | 181 |
| Hpy188I | TCNGA | 1 cut(s) | 61 |
| Hpy188III | TCNNGA | 1 cut(s) | 206 |
| Hpy8I | GTNNAC | 1 cut(s) | 181 |
| HpyAV | CCTTC | 1 cut(s) | 117 |
| HpyCH4III | ACNGT | 1 cut(s) | 178 |
| HpyCH4IV | ACGT | 1 cut(s) | 25 |
| HpyCH4V | TGCA | 3 cut(s) | 19, 48, 97 |
| HpyF3I | CTNAG | 1 cut(s) | 138 |
| HpySE526I | ACGT | 1 cut(s) | 25 |
| Hsp92I | GRCGYC | 1 cut(s) | 69 |
| Ksp22I | TGATCA | 1 cut(s) | 28 |
| Kzo9I | GATC | 2 cut(s) | 28, 279 |
| LpnPI | CCDG | 2 cut(s) | 169, 294 |
| Lsp1109I | GCAGC | 1 cut(s) | 284 |
| LweI | GCATC | 1 cut(s) | 124 |
| MaeI | CTAG | 1 cut(s) | 114 |
| MaeII | ACGT | 1 cut(s) | 25 |
| MalI | GATC | 2 cut(s) | 30, 281 |
| MboI | GATC | 2 cut(s) | 28, 279 |
| MluCI | AATT | 3 cut(s) | 62, 98, 241 |
| MmeI | TCCRAC | 1 cut(s) | 100 |
| MnlI | CCTC | 3 cut(s) | 83, 97, 202 |
| MslI | CAYNNNNRTG | 1 cut(s) | 24 |
| Mva1269I | GAATGC | 1 cut(s) | 212 |
| NdeII | GATC | 2 cut(s) | 28, 279 |
| OliI | CACNNNNGTG | 1 cut(s) | 24 |
| PctI | GAATGC | 1 cut(s) | 212 |
| PfeI | GAWTC | 1 cut(s) | 158 |
| PkrI | GCNGC | 1 cut(s) | 274 |
| PsiI | TTATAA | 1 cut(s) | 39 |
| RsaI | GTAC | 2 cut(s) | 171, 225 |
| RsaNI | GTAC | 2 cut(s) | 170, 224 |
| RseI | CAYNNNNRTG | 1 cut(s) | 24 |
| SatI | GCNGC | 1 cut(s) | 273 |
| Sau3AI | GATC | 2 cut(s) | 28, 279 |
| SetI | ASST | 7 cut(s) | 28, 45, 115, 149, 175, 229, 277 |
| SfaNI | GCATC | 1 cut(s) | 124 |
| SgeI | CNNG | 8 cut(s) | 28, 38, 126, 167, 196, 218, 260, 280 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 24 |
| SmlI | CTYRAG | 1 cut(s) | 206 |
| SmoI | CTYRAG | 1 cut(s) | 206 |
| Sse9I | AATT | 3 cut(s) | 62, 98, 241 |
| SspMI | CTAG | 1 cut(s) | 114 |
| TaaI | ACNGT | 1 cut(s) | 178 |
| TaiI | ACGT | 1 cut(s) | 28 |
| TasI | AATT | 3 cut(s) | 62, 98, 241 |
| TfiI | GAWTC | 1 cut(s) | 158 |
| TseI | GCWGC | 1 cut(s) | 272 |
| TspDTI | ATGAA | 1 cut(s) | 17 |
| XapI | RAATTY | 1 cut(s) | 62 |
| XspI | CTAG | 1 cut(s) | 114 |