Rw1G015980

26S proteasome non-ATPase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0001418
Physical Location & Seq
Reverse (-)
35648827 .. 35653115
4289 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G015980.1

Sequence Viewer

Length: 741 bp
ATGAGTATTACCTCCATTCCCAAATTGCCTAGCGTTGACTCATCGGAGGAAGGTAAAGAAGATAGTGGCTATGTTGTGGATGAGTACACTGTTCGAGTTGTGGATGTCTTTGCTATGTCGCAGAGTGGTACTAGTGTTAGTGTTGAAGCAGTGGATCATGTGTTCCAGACTAACATGCTCGATATGCTTAAGCAGATTGGAAGACCAGAGATGGTTGTAGGGTGGTACCATTCGCATCCTAGATTTGGCTGTTGGCTATCTGGTGTAGACATTAATACACAGCAGAGCTTTGAAGCTTTGAATCAACATGCTGTTGCTGTGGTGGTGGATCCAATTCAGAGTGTGAAGAGGAAGGTGGTCATTGATGCCTTCCGCCTTATCAACCCACAGACTATGATGCTCGGTCAAGAACCTCGGTATACCACATCCAATCTGGGCCATCTTAATAAACCATCTATTCAATACTCTATAGCAATCAATTACAGGAAGAATGAACTCGAGGAGAAGATGTTGCTTAACCTTCATAAGAAGGGACAGTTGCCAGTGTCATCCATGCAGGCAAACTTTGTTAATGATGATGTCCGATGGAGACAAGTTGTGCCCATCTACGTAGATAATGAATTTGGTGAGGGAATCATACCTTATTTGACTGATGCCTTGCAAGAGGTTGATGCTCATGTTCCCTACCACAGTGTAATTTCCTCCTTAGCCACTGACGAACAAATTGTTGATGAGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
JAB MitMem_reg

Protein Analysis

246

Amino Acids

27.71

Weight (kDa)

4.95

Isoelectric Point (pI)

36.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JAB PF01398 23 - 102 2.5e-22 JAB1/Mov34/MPN/PAD-1 ubiquitin protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 225
AccB1I GGYRCC 1 cut(s) 225
AccI GTMKAC 2 cut(s) 267, 419
AciI CCGC 1 cut(s) 373
AclWI GGATC 3 cut(s) 162, 323, 336
AcsI RAATTY 1 cut(s) 620
AfaI GTAC 3 cut(s) 86, 130, 227
AfiI CCNNNNNNNGG 1 cut(s) 245
AflII CTTAAG 1 cut(s) 188
AgsI TTSAA 4 cut(s) 146, 293, 301, 461
AhlI ACTAGT 1 cut(s) 131
AloI GAACNNNNNNTCC 2 cut(s) 146, 178
AluBI AGCT 3 cut(s) 288, 296, 736
AluI AGCT 3 cut(s) 288, 296, 736
Alw26I GTCTC 1 cut(s) 583
AlwI GGATC 3 cut(s) 162, 323, 336
Ama87I CYCGRG 1 cut(s) 497
AoxI GGCC 1 cut(s) 436
ApoI RAATTY 1 cut(s) 620
AseI ATTAAT 1 cut(s) 273
Asp718I GGTACC 1 cut(s) 225
AspS9I GGNCC 1 cut(s) 436
AsuHPI GGTGA 1 cut(s) 638
AvaI CYCGRG 1 cut(s) 497
BaeGI GKGCMC 1 cut(s) 603
BaeI ACNNNNGTAYC 2 cut(s) 120, 153
BamHI GGATCC 1 cut(s) 328
BanI GGYRCC 1 cut(s) 225
BbsI GAAGAC 1 cut(s) 208
BccI CCATC 5 cut(s) 205, 447, 460, 579, 611
BcoDI GTCTC 1 cut(s) 583
BcuI ACTAGT 1 cut(s) 131
BfaI CTAG 3 cut(s) 30, 132, 240
BfmI CTRYAG 1 cut(s) 468
BfrI CTTAAG 1 cut(s) 188
BmeT110I CYCGRG 1 cut(s) 497
BmgT120I GGNCC 1 cut(s) 436
BmiI GGNNCC 2 cut(s) 227, 330
BmsI GCATC 5 cut(s) 244, 355, 387, 643, 661
BpiI GAAGAC 1 cut(s) 208
Bpu10I CCTNAGC 1 cut(s) 706
BsaAI YACGTR 1 cut(s) 610
BsaJI CCNNGG 1 cut(s) 413
BsaXI ACNNNNNCTCC 2 cut(s) 494, 524
Bsc4I CCNNNNNNNGG 1 cut(s) 245
Bse1I ACTGG 1 cut(s) 542
BseDI CCNNGG 1 cut(s) 413
BseGI GGATG 5 cut(s) 85, 109, 235, 425, 548
BseLI CCNNNNNNNGG 1 cut(s) 245
BseNI ACTGG 1 cut(s) 542
BseRI GAGGAG 1 cut(s) 515
BseSI GKGCMC 1 cut(s) 603
BshFI GGCC 1 cut(s) 438
BshNI GGYRCC 1 cut(s) 225
BsiHKCI CYCGRG 1 cut(s) 497
BslFI GGGAC 1 cut(s) 546
BslI CCNNNNNNNGG 1 cut(s) 245
BsmAI GTCTC 1 cut(s) 583
BsmFI GGGAC 1 cut(s) 546
BsnI GGCC 1 cut(s) 438
BsoBI CYCGRG 1 cut(s) 497
Bsp1286I GDGCHC 1 cut(s) 603
Bsp143I GATC 2 cut(s) 154, 328
BspACI CCGC 1 cut(s) 373
BspANI GGCC 1 cut(s) 438
BspLI GGNNCC 2 cut(s) 227, 330
BspPI GGATC 3 cut(s) 162, 323, 336
BspT107I GGYRCC 1 cut(s) 225
BspTI CTTAAG 1 cut(s) 188
BsrI ACTGG 1 cut(s) 542
BssECI CCNNGG 1 cut(s) 413
BssMI GATC 2 cut(s) 154, 328
BssNAI GTATAC 1 cut(s) 420
Bst1107I GTATAC 1 cut(s) 420
Bst4CI ACNGT 3 cut(s) 91, 537, 692
Bst6I CTCTTC 1 cut(s) 341
BstAFI CTTAAG 1 cut(s) 188
BstBAI YACGTR 1 cut(s) 610
BstC8I GCNNGC 1 cut(s) 558
BstDEI CTNAG 1 cut(s) 706
BstF5I GGATG 5 cut(s) 85, 109, 235, 425, 548
BstKTI GATC 2 cut(s) 157, 331
BstMAI GTCTC 1 cut(s) 583
BstMBI GATC 2 cut(s) 154, 328
BstMWI GCNNNNNNNGC 1 cut(s) 184
BstNSI RCATGY 2 cut(s) 178, 311
BstSFI CTRYAG 1 cut(s) 468
BstSLI GKGCMC 1 cut(s) 603
BstSNI TACGTA 1 cut(s) 610
BstV2I GAAGAC 1 cut(s) 208
BstX2I RGATCY 1 cut(s) 328
BstYI RGATCY 1 cut(s) 328
BstZ17I GTATAC 1 cut(s) 420
BsuRI GGCC 1 cut(s) 438
BtsCI GGATG 5 cut(s) 85, 109, 235, 425, 548
BtsI GCAGTG 1 cut(s) 156
BtsIMutI CAGTG 5 cut(s) 87, 156, 549, 697, 711
Cac8I GCNNGC 1 cut(s) 558
Cfr13I GGNCC 1 cut(s) 436
Csp6I GTAC 3 cut(s) 85, 129, 226
CviAII CATG 5 cut(s) 158, 175, 308, 553, 677
CviJI RGCY 8 cut(s) 69, 249, 256, 288, 296, 438, 710, 736
CviKI_1 RGCY 8 cut(s) 69, 249, 256, 288, 296, 438, 710, 736
CviQI GTAC 3 cut(s) 85, 129, 226
DdeI CTNAG 1 cut(s) 706
DpnI GATC 2 cut(s) 156, 330
DpnII GATC 2 cut(s) 154, 328
Eam1104I CTCTTC 1 cut(s) 341
EarI CTCTTC 1 cut(s) 341
EciI GGCGGA 1 cut(s) 362
Eco105I TACGTA 1 cut(s) 610
Eco88I CYCGRG 1 cut(s) 497
FaeI CATG 5 cut(s) 161, 178, 311, 556, 680
FaqI GGGAC 1 cut(s) 546
FatI CATG 5 cut(s) 157, 174, 307, 552, 676
FblI GTMKAC 2 cut(s) 267, 419
FokI GGATG 5 cut(s) 92, 116, 222, 412, 535
FspBI CTAG 3 cut(s) 30, 132, 240
HaeIII GGCC 1 cut(s) 438
Hin1II CATG 5 cut(s) 161, 178, 311, 556, 680
HincII GTYRAC 1 cut(s) 37
HindII GTYRAC 1 cut(s) 37
HindIII AAGCTT 1 cut(s) 294
HinfI GANTC 3 cut(s) 38, 301, 633
HphI GGTGA 1 cut(s) 638
Hpy166II GTNNAC 4 cut(s) 37, 87, 268, 420
Hpy188I TCNGA 3 cut(s) 46, 339, 584
Hpy188III TCNNGA 2 cut(s) 166, 407
Hpy8I GTNNAC 4 cut(s) 37, 87, 268, 420
HpyAV CCTTC 5 cut(s) 44, 346, 379, 523, 530
HpyCH4III ACNGT 3 cut(s) 91, 537, 692
HpyCH4IV ACGT 1 cut(s) 609
HpyCH4V TGCA 2 cut(s) 556, 661
HpyF10VI GCNNNNNNNGC 1 cut(s) 184
HpyF3I CTNAG 1 cut(s) 706
HpySE526I ACGT 1 cut(s) 609
Hsp92II CATG 5 cut(s) 161, 178, 311, 556, 680
KpnI GGTACC 1 cut(s) 229
Kzo9I GATC 2 cut(s) 154, 328
LpnPI CCDG 7 cut(s) 179, 219, 246, 419, 469, 542, 555
LweI GCATC 5 cut(s) 244, 355, 387, 643, 661
MaeI CTAG 3 cut(s) 30, 132, 240
MaeII ACGT 1 cut(s) 609
MalI GATC 2 cut(s) 156, 330
MboI GATC 2 cut(s) 154, 328
MboII GAAGA 5 cut(s) 71, 213, 358, 499, 517
MflI RGATCY 1 cut(s) 328
MhlI GDGCHC 1 cut(s) 603
MluCI AATT 6 cut(s) 23, 333, 478, 620, 696, 723
MlyI GAGTC 1 cut(s) 32
MnlI CCTC 8 cut(s) 22, 40, 342, 423, 493, 622, 658, 712
MseI TTAA 5 cut(s) 189, 273, 444, 516, 570
MspCI CTTAAG 1 cut(s) 188
MwoI GCNNNNNNNGC 1 cut(s) 184
NdeII GATC 2 cut(s) 154, 328
NlaIII CATG 5 cut(s) 161, 178, 311, 556, 680
NlaIV GGNNCC 2 cut(s) 227, 330
NspI RCATGY 2 cut(s) 178, 311
PaeR7I CTCGAG 1 cut(s) 497
PfeI GAWTC 2 cut(s) 301, 633
PleI GAGTC 1 cut(s) 32
PpsI GAGTC 1 cut(s) 32
Ppu21I YACGTR 1 cut(s) 610
PshBI ATTAAT 1 cut(s) 273
PspN4I GGNNCC 2 cut(s) 227, 330
PspPI GGNCC 1 cut(s) 436
PspXI VCTCGAGB 1 cut(s) 497
PsuI RGATCY 1 cut(s) 328
RsaI GTAC 3 cut(s) 86, 130, 227
RsaNI GTAC 3 cut(s) 85, 129, 226
SaqAI TTAA 5 cut(s) 189, 273, 444, 516, 570
Sau3AI GATC 2 cut(s) 154, 328
Sau96I GGNCC 1 cut(s) 436
SchI GAGTC 1 cut(s) 32
SduI GDGCHC 1 cut(s) 603
SfaNI GCATC 5 cut(s) 244, 355, 387, 643, 661
SfcI CTRYAG 1 cut(s) 468
Sfr274I CTCGAG 1 cut(s) 497
SlaI CTCGAG 1 cut(s) 497
SmlI CTYRAG 2 cut(s) 188, 497
SmoI CTYRAG 2 cut(s) 188, 497
SnaBI TACGTA 1 cut(s) 610
SpeI ACTAGT 1 cut(s) 131
Sse9I AATT 6 cut(s) 23, 333, 478, 620, 696, 723
SsiI CCGC 1 cut(s) 373
SspMI CTAG 3 cut(s) 30, 132, 240
TaaI ACNGT 3 cut(s) 91, 537, 692
TaiI ACGT 1 cut(s) 612
TaqI TCGA 3 cut(s) 94, 180, 498
TaqII GACCGA 1 cut(s) 392
TasI AATT 6 cut(s) 23, 333, 478, 620, 696, 723
TatI WGTACW 1 cut(s) 84
TfiI GAWTC 2 cut(s) 301, 633
Tru1I TTAA 5 cut(s) 189, 273, 444, 516, 570
Tru9I TTAA 5 cut(s) 189, 273, 444, 516, 570
TscAI CASTG 5 cut(s) 94, 156, 549, 697, 718
TspDTI ATGAA 3 cut(s) 507, 512, 633
TspRI CASTG 5 cut(s) 94, 156, 549, 697, 718
Vha464I CTTAAG 1 cut(s) 188
VspI ATTAAT 1 cut(s) 273
XapI RAATTY 1 cut(s) 620
XceI RCATGY 2 cut(s) 178, 311
XcmI CCANNNNNNNNNTGG 1 cut(s) 430
XhoI CTCGAG 1 cut(s) 497
XmiI GTMKAC 2 cut(s) 267, 419
XspI CTAG 3 cut(s) 30, 132, 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.