AT1G58525

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001158
Physical Location & Seq
Forward (+)
21729913 .. 21731344
1432 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G58525.1

Sequence Viewer

Length: 1029 bp
ATGAAGCTTCAAATTCTATTGCTTGCTTTGGTTTTAATCGCTGTTGAAGCGAATGCAGTCAAGCAAGGGATAAACGCAACGATCCCAGCGCTAATAGTTTTTGGAGATTCAATAATGGATACAGGAAATAACAATAATCTTCCGACTCTTCTTAAATGTAACTTTCCTCCATATGGTAAAGATTATCCTGGAGGCGACGCCACTGGAAGATTTTCTGATGGAAGAGTTCCTTCTGATCTTATTGCGGAAAAGTTGGGATTGGCTAAGACATTACCAGCATATATGAATTCATATTTGAAGCCTGAGGATCTTCTTAAAGGTGTAACATTTGCATCTCGAGGAACTGGTTATGATCCATTGACAGCAAAAATTATGTCAGTGATATCGGTATGGGATCAACTCATATATTTCAAAGAATATATATCAAAGATCAAGAGGCATTTCGGAGAAGAAAAAGCCAAAGATATCTTGGAACATAGTTTCTTTCTTGTGGTGTCTAGTAGCAATGACCTTGCTCACACTTATTTAGCTCAAGCTCATAGATATGACCGTACCTCTTATGCCAATTTCTTGGCTGACTCTGCTGTCCATTTCGTGAGAGAATTACATAAGCTTGGAGCTCGAAAAATTGGAGTGTTTAGTGCAGTCCCCGTTGGTTGTGTTCCATTACAAAGAACAGTTTTTGGAGGTTTTTTTACAAGAGGATGTAATGAACCTCTAAACAACATGGCAAAACAATTCAACGCAAGACTTTCTCCAGCATTAGATTCTTTAGATAAAGAGTTGGATGGTGTTATCCTTTACATTAATGTTTATGATACTCTTTTTGACATGATCCAACACCCTAAAAAATACGGATGTTGCGGTAAAGGACTGCTCACAATATCCTATTTATGTAACTCATTGAACCCATTTACGTGTTCTAATTCTTCATCATACATATTTTGGGATAGCTATCATCCATCTGAAAGAGCTTATCAAGTTATCGTCGACAATTTACTCGATAAATATTTGAGCAAAGTCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

342

Amino Acids

38.11

Weight (kDa)

8.32

Isoelectric Point (pI)

27.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 32 - 332 1.9e-25 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 584
AccI GTMKAC 1 cut(s) 990
AciI CCGC 2 cut(s) 245, 864
AclWI GGATC 5 cut(s) 76, 315, 347, 402, 829
AcsI RAATTY 2 cut(s) 12, 286
AcyI GRCGYC 1 cut(s) 198
AfaI GTAC 1 cut(s) 553
AfeI AGCGCT 1 cut(s) 90
AfiI CCNNNNNNNGG 1 cut(s) 173
AflIII ACRYGT 1 cut(s) 917
AgsI TTSAA 7 cut(s) 11, 47, 111, 298, 412, 742, 907
AjnI CCWGG 1 cut(s) 187
AluBI AGCT 7 cut(s) 7, 530, 536, 613, 620, 954, 974
AluI AGCT 7 cut(s) 7, 530, 536, 613, 620, 954, 974
Alw21I GWGCWC 1 cut(s) 622
AlwI GGATC 5 cut(s) 76, 315, 347, 402, 829
Ama87I CYCGRG 1 cut(s) 336
Aor51HI AGCGCT 1 cut(s) 90
ApoI RAATTY 2 cut(s) 12, 286
ArsI GACNNNNNNTTYG 2 cut(s) 359, 391
AseI ATTAAT 1 cut(s) 807
Asp700I GAANNNNTTC 1 cut(s) 211
AspLEI GCGC 1 cut(s) 91
AvaI CYCGRG 1 cut(s) 336
AxyI CCTNAGG 1 cut(s) 303
BanII GRGCYC 1 cut(s) 622
Bbv12I GWGCWC 1 cut(s) 622
BccI CCATC 3 cut(s) 212, 782, 970
BciT130I CCWGG 1 cut(s) 189
BciVI GTATCC 1 cut(s) 112
BfaI CTAG 1 cut(s) 498
BfoI RGCGCY 1 cut(s) 92
BfuI GTATCC 1 cut(s) 112
Bme1390I CCNGG 1 cut(s) 189
BmeT110I CYCGRG 1 cut(s) 336
BmrFI CCNGG 1 cut(s) 189
BmsI GCATC 1 cut(s) 341
BpmI CTGGAG 2 cut(s) 210, 741
BpuEI CTTGAG 1 cut(s) 516
BsaAI YACGTR 1 cut(s) 918
BsaBI GATNNNNATC 1 cut(s) 954
BsaHI GRCGYC 1 cut(s) 198
Bsc4I CCNNNNNNNGG 1 cut(s) 173
Bse1I ACTGG 2 cut(s) 208, 349
Bse21I CCTNAGG 1 cut(s) 303
Bse3DI GCAATG 1 cut(s) 511
Bse8I GATNNNNATC 1 cut(s) 954
BseBI CCWGG 1 cut(s) 189
BseGI GGATG 4 cut(s) 710, 793, 863, 958
BseJI GATNNNNATC 1 cut(s) 954
BseLI CCNNNNNNNGG 1 cut(s) 173
BseMI GCAATG 1 cut(s) 511
BseMII CTCAG 1 cut(s) 294
BseNI ACTGG 2 cut(s) 208, 349
BseYI CCCAGC 1 cut(s) 85
BsgI GTGCAG 1 cut(s) 663
BsiHKAI GWGCWC 1 cut(s) 622
BsiHKCI CYCGRG 1 cut(s) 336
BslFI GGGAC 1 cut(s) 632
BslI CCNNNNNNNGG 1 cut(s) 173
BsmFI GGGAC 1 cut(s) 632
BsmI GAATGC 1 cut(s) 58
BsoBI CYCGRG 1 cut(s) 336
Bsp1286I GDGCHC 1 cut(s) 622
Bsp143I GATC 7 cut(s) 81, 235, 307, 352, 394, 429, 834
BspACI CCGC 2 cut(s) 245, 864
BspCNI CTCAG 1 cut(s) 295
BspPI GGATC 5 cut(s) 76, 315, 347, 402, 829
BsrDI GCAATG 1 cut(s) 511
BsrI ACTGG 2 cut(s) 208, 349
BssMI GATC 7 cut(s) 81, 235, 307, 352, 394, 429, 834
BssNI GRCGYC 1 cut(s) 198
Bst2UI CCWGG 1 cut(s) 189
Bst4CI ACNGT 2 cut(s) 551, 679
Bst6I CTCTTC 2 cut(s) 153, 217
BstACI GRCGYC 1 cut(s) 198
BstBAI YACGTR 1 cut(s) 918
BstC8I GCNNGC 1 cut(s) 24
BstDEI CTNAG 2 cut(s) 264, 303
BstF5I GGATG 4 cut(s) 710, 793, 863, 958
BstH2I RGCGCY 1 cut(s) 92
BstHHI GCGC 1 cut(s) 91
BstKTI GATC 7 cut(s) 84, 238, 310, 355, 397, 432, 837
BstMBI GATC 7 cut(s) 81, 235, 307, 352, 394, 429, 834
BstMWI GCNNNNNNNGC 2 cut(s) 47, 581
BstNI CCWGG 1 cut(s) 189
BstSCI CCNGG 1 cut(s) 187
BstX2I RGATCY 1 cut(s) 307
BstXI CCANNNNNNTGG 1 cut(s) 571
BstYI RGATCY 1 cut(s) 307
Bsu36I CCTNAGG 1 cut(s) 303
BsuI GTATCC 1 cut(s) 112
BtsCI GGATG 4 cut(s) 710, 793, 863, 958
BtsIMutI CAGTG 2 cut(s) 201, 384
Cac8I GCNNGC 1 cut(s) 24
CfoI GCGC 1 cut(s) 91
CseI GACGC 1 cut(s) 206
Csp6I GTAC 1 cut(s) 552
CviAII CATG 2 cut(s) 727, 832
CviQI GTAC 1 cut(s) 552
DdeI CTNAG 2 cut(s) 264, 303
DpnI GATC 7 cut(s) 83, 237, 309, 354, 396, 431, 836
DpnII GATC 7 cut(s) 81, 235, 307, 352, 394, 429, 834
DrdI GACNNNNNNGTC 1 cut(s) 584
DseDI GACNNNNNNGTC 1 cut(s) 584
Eam1104I CTCTTC 2 cut(s) 153, 217
EarI CTCTTC 2 cut(s) 153, 217
Ecl136II GAGCTC 1 cut(s) 620
Eco24I GRGCYC 1 cut(s) 622
Eco32I GATATC 2 cut(s) 384, 466
Eco47III AGCGCT 1 cut(s) 90
Eco53kI GAGCTC 1 cut(s) 620
Eco81I CCTNAGG 1 cut(s) 303
Eco88I CYCGRG 1 cut(s) 336
EcoICRI GAGCTC 1 cut(s) 620
EcoRI GAATTC 1 cut(s) 286
EcoRII CCWGG 1 cut(s) 187
EcoRV GATATC 2 cut(s) 384, 466
EcoT38I GRGCYC 1 cut(s) 622
FaeI CATG 2 cut(s) 730, 835
FalI AAGNNNNNCTT 2 cut(s) 214, 246
FaqI GGGAC 1 cut(s) 632
FatI CATG 2 cut(s) 726, 831
FauNDI CATATG 1 cut(s) 172
FblI GTMKAC 1 cut(s) 990
FokI GGATG 4 cut(s) 717, 800, 870, 945
FriOI GRGCYC 1 cut(s) 622
FspBI CTAG 1 cut(s) 498
GlaI GCGC 1 cut(s) 90
GsaI CCCAGC 1 cut(s) 89
GsuI CTGGAG 2 cut(s) 210, 741
HaeII RGCGCY 1 cut(s) 92
HgaI GACGC 1 cut(s) 206
HhaI GCGC 1 cut(s) 91
Hin1I GRCGYC 1 cut(s) 198
Hin1II CATG 2 cut(s) 730, 835
Hin6I GCGC 1 cut(s) 89
HinP1I GCGC 1 cut(s) 89
HincII GTYRAC 1 cut(s) 991
HindII GTYRAC 1 cut(s) 991
HindIII AAGCTT 2 cut(s) 5, 611
HinfI GANTC 4 cut(s) 107, 145, 578, 767
Hpy166II GTNNAC 1 cut(s) 991
Hpy188I TCNGA 5 cut(s) 144, 217, 235, 446, 967
Hpy188III TCNNGA 3 cut(s) 336, 433, 595
Hpy8I GTNNAC 1 cut(s) 991
Hpy99I CGWCG 2 cut(s) 200, 992
HpyAV CCTTC 1 cut(s) 240
HpyCH4III ACNGT 2 cut(s) 551, 679
HpyCH4IV ACGT 1 cut(s) 917
HpyCH4V TGCA 3 cut(s) 56, 332, 644
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 581
HpyF3I CTNAG 2 cut(s) 264, 303
HpySE526I ACGT 1 cut(s) 917
Hsp92I GRCGYC 1 cut(s) 198
Hsp92II CATG 2 cut(s) 730, 835
HspAI GCGC 1 cut(s) 89
Kzo9I GATC 7 cut(s) 81, 235, 307, 352, 394, 429, 834
LmnI GCTCC 1 cut(s) 617
LpnPI CCDG 9 cut(s) 99, 108, 174, 189, 201, 288, 315, 330, 771
LweI GCATC 1 cut(s) 341
MaeI CTAG 1 cut(s) 498
MaeII ACGT 1 cut(s) 917
MaeIII GTNAC 3 cut(s) 158, 322, 896
MalI GATC 7 cut(s) 83, 237, 309, 354, 396, 431, 836
MboI GATC 7 cut(s) 81, 235, 307, 352, 394, 429, 834
MboII GAAGA 7 cut(s) 131, 140, 219, 234, 302, 461, 921
MflI RGATCY 1 cut(s) 307
MhlI GDGCHC 1 cut(s) 622
MluCI AATT 9 cut(s) 12, 286, 369, 565, 602, 627, 737, 925, 994
MlyI GAGTC 2 cut(s) 139, 572
MmeI TCCRAC 3 cut(s) 167, 765, 862
MnlI CCTC 9 cut(s) 177, 185, 298, 332, 429, 565, 680, 695, 726
MroXI GAANNNNTTC 1 cut(s) 211
MseI TTAA 4 cut(s) 35, 153, 315, 807
MslI CAYNNNNRTG 2 cut(s) 543, 916
MspR9I CCNGG 1 cut(s) 189
Mva1269I GAATGC 1 cut(s) 58
MvaI CCWGG 1 cut(s) 189
MwoI GCNNNNNNNGC 2 cut(s) 47, 581
NdeI CATATG 1 cut(s) 172
NdeII GATC 7 cut(s) 81, 235, 307, 352, 394, 429, 834
NlaIII CATG 2 cut(s) 730, 835
PaeR7I CTCGAG 1 cut(s) 336
PctI GAATGC 1 cut(s) 58
PdmI GAANNNNTTC 1 cut(s) 211
PfeI GAWTC 2 cut(s) 107, 767
PfoI TCCNGGA 1 cut(s) 187
PleI GAGTC 2 cut(s) 139, 572
PpsI GAGTC 2 cut(s) 139, 572
Ppu21I YACGTR 1 cut(s) 918
PshBI ATTAAT 1 cut(s) 807
Psp124BI GAGCTC 1 cut(s) 622
Psp6I CCWGG 1 cut(s) 187
PspFI CCCAGC 1 cut(s) 85
PspGI CCWGG 1 cut(s) 187
PsuI RGATCY 1 cut(s) 307
RsaI GTAC 1 cut(s) 553
RsaNI GTAC 1 cut(s) 552
RseI CAYNNNNRTG 2 cut(s) 543, 916
SacI GAGCTC 1 cut(s) 622
SalI GTCGAC 1 cut(s) 989
SaqAI TTAA 4 cut(s) 35, 153, 315, 807
Sau3AI GATC 7 cut(s) 81, 235, 307, 352, 394, 429, 834
SchI GAGTC 2 cut(s) 139, 572
ScrFI CCNGG 1 cut(s) 189
SduI GDGCHC 1 cut(s) 622
SfaNI GCATC 1 cut(s) 341
Sfr274I CTCGAG 1 cut(s) 336
SlaI CTCGAG 1 cut(s) 336
SmiMI CAYNNNNRTG 2 cut(s) 543, 916
SmlI CTYRAG 2 cut(s) 336, 531
SmoI CTYRAG 2 cut(s) 336, 531
Sse9I AATT 9 cut(s) 12, 286, 369, 565, 602, 627, 737, 925, 994
SsiI CCGC 2 cut(s) 245, 864
SspI AATATT 1 cut(s) 1010
SspMI CTAG 1 cut(s) 498
SstI GAGCTC 1 cut(s) 622
StyD4I CCNGG 1 cut(s) 187
TaaI ACNGT 2 cut(s) 551, 679
TaiI ACGT 1 cut(s) 920
TaqI TCGA 4 cut(s) 337, 622, 990, 1002
TasI AATT 9 cut(s) 12, 286, 369, 565, 602, 627, 737, 925, 994
TfiI GAWTC 2 cut(s) 107, 767
Tru1I TTAA 4 cut(s) 35, 153, 315, 807
Tru9I TTAA 4 cut(s) 35, 153, 315, 807
TscAI CASTG 2 cut(s) 208, 384
TspDTI ATGAA 5 cut(s) 17, 279, 299, 726, 921
TspGWI ACGGA 1 cut(s) 870
TspRI CASTG 2 cut(s) 208, 384
VspI ATTAAT 1 cut(s) 807
XapI RAATTY 2 cut(s) 12, 286
XcmI CCANNNNNNNNNTGG 1 cut(s) 466
XhoI CTCGAG 1 cut(s) 336
XmiI GTMKAC 1 cut(s) 990
XmnI GAANNNNTTC 1 cut(s) 211
XspI CTAG 1 cut(s) 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.