AT1G75900

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001158
Physical Location & Seq
Forward (+)
28498821 .. 28501254
2434 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G75900.1

Sequence Viewer

Length: 1095 bp
ATGAAAGACAATTCAAGCTGGTCTTGTTCTTGTTCTTGGTCTTCGTGGAAAATATGTTTGTTGTCTGTTCTCTTTCTCACCGAGACGATCACCGCCGTGAAGCTGCCGCCAAAATTGATTATTCCGGCTGTAATCGCTTTCGGAGACTCCATCGTCGACACAGGAATGAACAACAATGTCAAAACCGTGGTTAAGTGTGATTTTCTTCCTTATGGTATCAATTTCCAAAGCGGAGTTGCCACGGGGAGATTCTGCGATGGACGAGTCCCTGCCGATTTGCTAGCCGAAGAACTGGGAATAAAATCAATTGTACCAGCATACCTAGATCCGAATCTAAAATCGAAAGATCTTTTAACCGGTGTATCGTTTGCGTCCGGAGGTTCTGGTTATGATCCTATAACACCGAAACTTGTGGCAGTAATATCATTAGAAGATCAATTGAGTTATTTCGAGGAGTACATAGAGAAAGTGAAGAATATAGTTGGGGAAGCAAGAAAAGACTTCATAGTAGCCAACAGCTTATTCTTATTGGTCGCAGGCAGCGACGACATAGCCAATACATACTATACTCTACGTGCAAGACCTGAATACGACGTCGATTCATACACTACTCTTATGTCTGACTCTGCCTCAGAATTTGTGACTAAACTATATGGATATGGAGTGAGAAGAGTAGCTGTGTTTGGTGCACCACCAATTGGGTGTGTACCATCACAGAGAACGTTAGGAGGAGGTATCTTGAGAGATTGTGCTGATAATTACAACGAAGCAGCAAAACTTTTTAATTCAAAGCTCTCCCCAAAATTGGATTCGTTGCGTAAAACCCTACCGGGCATCAAACCGATCTACATTAATATCTATGATCCTCTTTTTGACATCATCCAGAATCCTGCAAATTATGGGTTTGAAGTGTCTAATAAAGGATGCTGTGGAACAGGAGCCATAGAAGTTGCTGTGTTGTGCAATAAAATCACATCTTCTGTATGTCCCGACGTGTCTACTCATGTGTTTTGGGACAGTTATCATCCTACAGAGAAAACTTACAAAGTATTAGTCTCACTGTTGATTAACAAATTTGTTAATCAGTTCGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

364

Amino Acids

39.82

Weight (kDa)

5.59

Isoelectric Point (pI)

35.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 44 - 353 7.8e-32 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 152
AatII GACGTC 1 cut(s) 597
AccB7I CCANNNNNTGG 1 cut(s) 698
AccI GTMKAC 2 cut(s) 156, 998
AccIII TCCGGA 1 cut(s) 374
AciI CCGC 3 cut(s) 93, 107, 231
AclI AACGTT 1 cut(s) 722
AclWI GGATC 3 cut(s) 320, 386, 857
AcsI RAATTY 2 cut(s) 635, 1073
AcyI GRCGYC 1 cut(s) 594
AfaI GTAC 3 cut(s) 312, 458, 708
AfiI CCNNNNNNNGG 2 cut(s) 698, 805
AflIII ACRYGT 1 cut(s) 993
AgeI ACCGGT 1 cut(s) 356
AgsI TTSAA 3 cut(s) 15, 789, 908
AjiI CACGTC 1 cut(s) 994
AjuI GAANNNNNNNTTGG 4 cut(s) 506, 538, 793, 825
AleI CACNNNNGTG 1 cut(s) 95
AluBI AGCT 5 cut(s) 18, 103, 519, 677, 793
AluI AGCT 5 cut(s) 18, 103, 519, 677, 793
Alw21I GWGCWC 1 cut(s) 691
Alw26I GTCTC 3 cut(s) 77, 138, 1060
Alw44I GTGCAC 1 cut(s) 687
AlwI GGATC 3 cut(s) 320, 386, 857
Aor13HI TCCGGA 1 cut(s) 374
ApaLI GTGCAC 1 cut(s) 687
ApeKI GCWGC 3 cut(s) 103, 540, 770
ApoI RAATTY 2 cut(s) 635, 1073
ArsI GACNNNNNNTTYG 2 cut(s) 1038, 1070
AseI ATTAAT 1 cut(s) 852
AsiGI ACCGGT 1 cut(s) 356
AsuC2I CCSGG 1 cut(s) 831
AsuHPI GGTGA 2 cut(s) 70, 82
AsuNHI GCTAGC 1 cut(s) 280
BaeGI GKGCMC 1 cut(s) 691
BaeI ACNNNNGTAYC 2 cut(s) 345, 378
BbsI GAAGAC 1 cut(s) 33
Bbv12I GWGCWC 1 cut(s) 691
BbvI GCAGC 3 cut(s) 90, 552, 782
BccI CCATC 3 cut(s) 158, 251, 718
BceAI ACGGC 1 cut(s) 80
BcnI CCSGG 1 cut(s) 831
BcoDI GTCTC 3 cut(s) 77, 138, 1060
BfaI CTAG 2 cut(s) 281, 323
BfmI CTRYAG 1 cut(s) 1029
BglII AGATCT 1 cut(s) 346
BisI GCNGC 4 cut(s) 104, 107, 541, 771
BlsI GCNGC 4 cut(s) 105, 108, 542, 772
Bme1390I CCNGG 1 cut(s) 831
BmgBI CACGTC 1 cut(s) 994
BmiI GGNNCC 1 cut(s) 940
BmrFI CCNGG 1 cut(s) 831
BmrI ACTGGG 1 cut(s) 302
BmsI GCATC 2 cut(s) 843, 914
BmtI GCTAGC 1 cut(s) 284
BmuI ACTGGG 1 cut(s) 302
BpiI GAAGAC 1 cut(s) 33
BpuEI CTTGAG 1 cut(s) 760
BpuMI CCSGG 1 cut(s) 831
BsaAI YACGTR 1 cut(s) 575
BsaBI GATNNNNATC 1 cut(s) 330
BsaHI GRCGYC 1 cut(s) 594
BsaJI CCNNGG 2 cut(s) 186, 240
BsaWI WCCGGW 2 cut(s) 356, 374
Bsc4I CCNNNNNNNGG 2 cut(s) 698, 805
Bse118I RCCGGY 1 cut(s) 356
Bse1I ACTGG 1 cut(s) 297
Bse8I GATNNNNATC 1 cut(s) 330
BseAI TCCGGA 1 cut(s) 374
BseDI CCNNGG 2 cut(s) 186, 240
BseGI GGATG 3 cut(s) 879, 929, 1024
BseJI GATNNNNATC 1 cut(s) 330
BseLI CCNNNNNNNGG 2 cut(s) 698, 805
BseMII CTCAG 1 cut(s) 645
BseNI ACTGG 1 cut(s) 297
BseRI GAGGAG 2 cut(s) 467, 744
BseSI GKGCMC 1 cut(s) 691
BseXI GCAGC 3 cut(s) 90, 552, 782
BshTI ACCGGT 1 cut(s) 356
BsiHKAI GWGCWC 1 cut(s) 691
BsiSI CCGG 4 cut(s) 125, 357, 375, 830
BslFI GGGAC 3 cut(s) 251, 972, 1028
BslI CCNNNNNNNGG 2 cut(s) 698, 805
BsmAI GTCTC 3 cut(s) 77, 138, 1060
BsmBI CGTCTC 1 cut(s) 77
BsmFI GGGAC 3 cut(s) 251, 972, 1028
Bsp1286I GDGCHC 1 cut(s) 691
Bsp13I TCCGGA 1 cut(s) 374
Bsp143I GATC 7 cut(s) 87, 325, 346, 391, 433, 843, 862
BspACI CCGC 3 cut(s) 93, 107, 231
BspCNI CTCAG 1 cut(s) 644
BspEI TCCGGA 1 cut(s) 374
BspLI GGNNCC 1 cut(s) 940
BspOI GCTAGC 1 cut(s) 284
BspPI GGATC 3 cut(s) 320, 386, 857
BsrFI RCCGGY 1 cut(s) 356
BsrI ACTGG 1 cut(s) 297
BssAI RCCGGY 1 cut(s) 356
BssECI CCNNGG 2 cut(s) 186, 240
BssMI GATC 7 cut(s) 87, 325, 346, 391, 433, 843, 862
BssNI GRCGYC 1 cut(s) 594
Bst4CI ACNGT 3 cut(s) 187, 1019, 1062
Bst6I CTCTTC 1 cut(s) 664
BstACI GRCGYC 1 cut(s) 594
BstBAI YACGTR 1 cut(s) 575
BstC8I GCNNGC 2 cut(s) 282, 538
BstDEI CTNAG 1 cut(s) 631
BstDSI CCRYGG 2 cut(s) 186, 240
BstF5I GGATG 3 cut(s) 879, 929, 1024
BstKTI GATC 7 cut(s) 90, 328, 349, 394, 436, 846, 865
BstMAI GTCTC 3 cut(s) 77, 138, 1060
BstMBI GATC 7 cut(s) 87, 325, 346, 391, 433, 843, 862
BstMWI GCNNNNNNNGC 1 cut(s) 134
BstSCI CCNGG 1 cut(s) 829
BstSFI CTRYAG 1 cut(s) 1029
BstSLI GKGCMC 1 cut(s) 691
BstV1I GCAGC 3 cut(s) 90, 552, 782
BstV2I GAAGAC 1 cut(s) 33
BstX2I RGATCY 2 cut(s) 325, 346
BstYI RGATCY 2 cut(s) 325, 346
BtgI CCRYGG 2 cut(s) 186, 240
BtgZI GCGATG 1 cut(s) 270
BtrI CACGTC 1 cut(s) 994
BtsCI GGATG 3 cut(s) 879, 929, 1024
BtsIMutI CAGTG 1 cut(s) 1058
Cac8I GCNNGC 2 cut(s) 282, 538
Cfr10I RCCGGY 1 cut(s) 356
CseI GACGC 1 cut(s) 360
Csp6I GTAC 3 cut(s) 311, 457, 707
CspAI ACCGGT 1 cut(s) 356
CviAII CATG 1 cut(s) 1004
CviQI GTAC 3 cut(s) 311, 457, 707
DdeI CTNAG 1 cut(s) 631
DpnI GATC 7 cut(s) 89, 327, 348, 393, 435, 845, 864
DpnII GATC 7 cut(s) 87, 325, 346, 391, 433, 843, 862
DrdI GACNNNNNNGTC 1 cut(s) 152
DseDI GACNNNNNNGTC 1 cut(s) 152
Eam1104I CTCTTC 1 cut(s) 664
EarI CTCTTC 1 cut(s) 664
Esp3I CGTCTC 1 cut(s) 77
FaeI CATG 1 cut(s) 1007
FalI AAGNNNNNCTT 2 cut(s) 7, 39
FaqI GGGAC 3 cut(s) 251, 972, 1028
FatI CATG 1 cut(s) 1003
FblI GTMKAC 2 cut(s) 156, 998
Fnu4HI GCNGC 4 cut(s) 104, 107, 541, 771
FokI GGATG 3 cut(s) 866, 936, 1011
Fsp4HI GCNGC 4 cut(s) 104, 107, 541, 771
FspBI CTAG 2 cut(s) 281, 323
GluI GCNGC 4 cut(s) 104, 107, 541, 771
HapII CCGG 4 cut(s) 125, 357, 375, 830
HgaI GACGC 1 cut(s) 360
Hin1I GRCGYC 1 cut(s) 594
Hin1II CATG 1 cut(s) 1007
HincII GTYRAC 1 cut(s) 157
HindII GTYRAC 1 cut(s) 157
HinfI GANTC 8 cut(s) 146, 249, 264, 331, 599, 623, 809, 886
HpaII CCGG 4 cut(s) 125, 357, 375, 830
HphI GGTGA 2 cut(s) 70, 82
Hpy166II GTNNAC 4 cut(s) 157, 689, 707, 999
Hpy188I TCNGA 5 cut(s) 143, 330, 622, 634, 1094
Hpy188III TCNNGA 4 cut(s) 375, 739, 883, 989
Hpy8I GTNNAC 4 cut(s) 157, 689, 707, 999
Hpy99I CGWCG 5 cut(s) 158, 548, 596, 599, 995
HpyCH4III ACNGT 3 cut(s) 187, 1019, 1062
HpyCH4IV ACGT 4 cut(s) 574, 594, 722, 993
HpyCH4V TGCA 4 cut(s) 578, 689, 893, 963
HpyF10VI GCNNNNNNNGC 1 cut(s) 134
HpyF3I CTNAG 1 cut(s) 631
HpySE526I ACGT 4 cut(s) 574, 594, 722, 993
Hsp92I GRCGYC 1 cut(s) 594
Hsp92II CATG 1 cut(s) 1007
Kpn2I TCCGGA 1 cut(s) 374
Kzo9I GATC 7 cut(s) 87, 325, 346, 391, 433, 843, 862
LmnI GCTCC 1 cut(s) 938
Lsp1109I GCAGC 3 cut(s) 90, 552, 782
LweI GCATC 2 cut(s) 843, 914
MaeI CTAG 2 cut(s) 281, 323
MaeII ACGT 4 cut(s) 574, 594, 722, 993
MaeIII GTNAC 1 cut(s) 640
MalI GATC 7 cut(s) 89, 327, 348, 393, 435, 845, 864
MboI GATC 7 cut(s) 87, 325, 346, 391, 433, 843, 862
MboII GAAGA 7 cut(s) 33, 197, 299, 443, 484, 681, 969
MfeI CAATTG 3 cut(s) 306, 437, 696
MflI RGATCY 2 cut(s) 325, 346
MhlI GDGCHC 1 cut(s) 691
MlyI GAGTC 3 cut(s) 140, 273, 617
MnlI CCTC 6 cut(s) 371, 445, 640, 722, 725, 876
MroI TCCGGA 1 cut(s) 374
MseI TTAA 6 cut(s) 192, 353, 783, 852, 1068, 1080
MslI CAYNNNNRTG 2 cut(s) 95, 164
MspI CCGG 4 cut(s) 125, 357, 375, 830
MspR9I CCNGG 1 cut(s) 831
MunI CAATTG 3 cut(s) 306, 437, 696
MwoI GCNNNNNNNGC 1 cut(s) 134
NciI CCSGG 1 cut(s) 831
NdeII GATC 7 cut(s) 87, 325, 346, 391, 433, 843, 862
NheI GCTAGC 1 cut(s) 280
NlaIII CATG 1 cut(s) 1007
NlaIV GGNNCC 1 cut(s) 940
NmuCI GTSAC 1 cut(s) 640
OliI CACNNNNGTG 1 cut(s) 95
PcsI WCGNNNNNNNCGW 1 cut(s) 540
PfeI GAWTC 5 cut(s) 249, 331, 599, 809, 886
PflMI CCANNNNNTGG 1 cut(s) 698
PinAI ACCGGT 1 cut(s) 356
PkrI GCNGC 4 cut(s) 105, 108, 542, 772
PleI GAGTC 3 cut(s) 140, 272, 617
PpsI GAGTC 3 cut(s) 140, 272, 617
Ppu21I YACGTR 1 cut(s) 575
PshBI ATTAAT 1 cut(s) 852
Psp1406I AACGTT 1 cut(s) 722
PspN4I GGNNCC 1 cut(s) 940
PsuI RGATCY 2 cut(s) 325, 346
RsaI GTAC 3 cut(s) 312, 458, 708
RsaNI GTAC 3 cut(s) 311, 457, 707
RseI CAYNNNNRTG 2 cut(s) 95, 164
SalI GTCGAC 1 cut(s) 155
SaqAI TTAA 6 cut(s) 192, 353, 783, 852, 1068, 1080
SatI GCNGC 4 cut(s) 104, 107, 541, 771
Sau3AI GATC 7 cut(s) 87, 325, 346, 391, 433, 843, 862
SchI GAGTC 3 cut(s) 140, 273, 617
ScrFI CCNGG 1 cut(s) 831
SduI GDGCHC 1 cut(s) 691
SfaNI GCATC 2 cut(s) 843, 914
SfcI CTRYAG 1 cut(s) 1029
SmiMI CAYNNNNRTG 2 cut(s) 95, 164
SmlI CTYRAG 1 cut(s) 739
SmoI CTYRAG 1 cut(s) 739
SsiI CCGC 3 cut(s) 93, 107, 231
SspMI CTAG 2 cut(s) 281, 323
StyD4I CCNGG 1 cut(s) 829
TaaI ACNGT 3 cut(s) 187, 1019, 1062
TaiI ACGT 4 cut(s) 577, 597, 725, 996
TaqI TCGA 4 cut(s) 156, 341, 450, 597
TatI WGTACW 1 cut(s) 456
TauI GCSGC 1 cut(s) 109
TfiI GAWTC 5 cut(s) 249, 331, 599, 809, 886
Tru1I TTAA 6 cut(s) 192, 353, 783, 852, 1068, 1080
Tru9I TTAA 6 cut(s) 192, 353, 783, 852, 1068, 1080
TscAI CASTG 1 cut(s) 1065
TseFI GTSAC 1 cut(s) 640
TseI GCWGC 3 cut(s) 103, 540, 770
Tsp45I GTSAC 1 cut(s) 640
TspDTI ATGAA 4 cut(s) 17, 182, 493, 591
TspRI CASTG 1 cut(s) 1065
Van91I CCANNNNNTGG 1 cut(s) 698
VneI GTGCAC 1 cut(s) 687
VspI ATTAAT 1 cut(s) 852
XapI RAATTY 2 cut(s) 635, 1073
XmiI GTMKAC 2 cut(s) 156, 998
XspI CTAG 2 cut(s) 281, 323
ZraI GACGTC 1 cut(s) 595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.