pycom15g00740

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0001158
Physical Location & Seq
Reverse (-)
402164 .. 402996
833 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g00740.3

Sequence Viewer

Length: 642 bp
ATGGAAAAGCTGAAAGGAATTGTTGGAGAGGAGAAAACAAACTTCATCATACAAAACAGTCTGATTCTTGTGGTAGCCGGCAGCAACGATATTTCTAATACCTATTTTCTTAGCGGTGTTAGGAAATTGGAGTATGACGTTCCTTCTTACACCGACTTCATGCTCAAATATGCCTCTGATTTTATCAAGGACTTGTATGGATTGGGGGCACGAAGAATAGGCGTACTAAGTGCACCACCTATCGGATGTGTGCCATCTCAACGAACGTTAAATGGAGGCATACAAAGAGATTGTGACGAAAAACAAAACCAAGCATCACAGCTGTTCAATTCCAAGCTCTCTGTGGAGATGGACCACCTTAACAAAAACCTGCCAAACTCGAGAGTGGTCTACATTGACATCTATAATCCGCTACTCGATCTCATAAACAACCCAGCTAAATACGGGTTTGAAGTTGCGAATAAAGGGTGTTGTGGTACTGGCACCATTGAAGTAACAAAACTGTGCAACCAGCTCCAACCTGCAGGAGTCTGCATAGATGACTCTAAGTATGTTTTTTGGGACAGCTACCATCCAACCGAAAGAGCGTACAAGATCATTGTGAAGCAGATTCTCGACAATTATCCCAGCCTCGTCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

214

Amino Acids

23.83

Weight (kDa)

6.13

Isoelectric Point (pI)

29.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 378, 529
AccB1I GGYRCC 1 cut(s) 482
AccI GTMKAC 1 cut(s) 390
AciI CCGC 2 cut(s) 114, 410
AclI AACGTT 1 cut(s) 266
AfaI GTAC 3 cut(s) 225, 478, 590
AfiI CCNNNNNNNGG 1 cut(s) 242
AgsI TTSAA 3 cut(s) 328, 452, 491
AluBI AGCT 6 cut(s) 10, 322, 337, 437, 514, 567
AluI AGCT 6 cut(s) 10, 322, 337, 437, 514, 567
Alw21I GWGCWC 1 cut(s) 235
Alw44I GTGCAC 1 cut(s) 231
Ama87I CYCGRG 1 cut(s) 379
ApaLI GTGCAC 1 cut(s) 231
ApeKI GCWGC 1 cut(s) 81
AspS9I GGNCC 1 cut(s) 352
AvaI CYCGRG 1 cut(s) 379
AvaII GGWCC 1 cut(s) 352
BaeGI GKGCMC 2 cut(s) 211, 235
BanI GGYRCC 1 cut(s) 482
Bbv12I GWGCWC 1 cut(s) 235
BbvI GCAGC 1 cut(s) 93
BccI CCATC 3 cut(s) 262, 343, 579
BfmI CTRYAG 1 cut(s) 522
BfuAI ACCTGC 2 cut(s) 378, 529
BisI GCNGC 1 cut(s) 82
BlsI GCNGC 1 cut(s) 83
Bme18I GGWCC 1 cut(s) 352
BmeT110I CYCGRG 1 cut(s) 379
BmgT120I GGNCC 1 cut(s) 352
BmiI GGNNCC 1 cut(s) 484
BmsI GCATC 1 cut(s) 323
Bsc4I CCNNNNNNNGG 1 cut(s) 242
Bse118I RCCGGY 1 cut(s) 77
Bse1I ACTGG 1 cut(s) 484
BseGI GGATG 2 cut(s) 251, 571
BseLI CCNNNNNNNGG 1 cut(s) 242
BseNI ACTGG 1 cut(s) 484
BseRI GAGGAG 1 cut(s) 44
BseSI GKGCMC 2 cut(s) 211, 235
BseXI GCAGC 1 cut(s) 93
BseYI CCCAGC 2 cut(s) 433, 626
BshNI GGYRCC 1 cut(s) 482
BsiHKAI GWGCWC 1 cut(s) 235
BsiHKCI CYCGRG 1 cut(s) 379
BsiSI CCGG 1 cut(s) 78
BslFI GGGAC 1 cut(s) 575
BslI CCNNNNNNNGG 1 cut(s) 242
BsmFI GGGAC 1 cut(s) 575
BsoBI CYCGRG 1 cut(s) 379
Bsp1286I GDGCHC 2 cut(s) 211, 235
Bsp143I GATC 2 cut(s) 418, 594
BspACI CCGC 2 cut(s) 114, 410
BspLI GGNNCC 1 cut(s) 484
BspMAI CTGCAG 1 cut(s) 526
BspMI ACCTGC 2 cut(s) 378, 529
BspT107I GGYRCC 1 cut(s) 482
BsrFI RCCGGY 1 cut(s) 77
BsrI ACTGG 1 cut(s) 484
BssAI RCCGGY 1 cut(s) 77
BssMI GATC 2 cut(s) 418, 594
Bst4CI ACNGT 2 cut(s) 59, 504
BstC8I GCNNGC 1 cut(s) 79
BstDEI CTNAG 3 cut(s) 110, 227, 546
BstF5I GGATG 2 cut(s) 251, 571
BstKTI GATC 2 cut(s) 421, 597
BstMBI GATC 2 cut(s) 418, 594
BstSFI CTRYAG 1 cut(s) 522
BstSLI GKGCMC 2 cut(s) 211, 235
BstV1I GCAGC 1 cut(s) 93
BtsCI GGATG 2 cut(s) 251, 571
BveI ACCTGC 2 cut(s) 378, 529
Cac8I GCNNGC 1 cut(s) 79
Cfr10I RCCGGY 1 cut(s) 77
Cfr13I GGNCC 1 cut(s) 352
Csp6I GTAC 3 cut(s) 224, 477, 589
CviAII CATG 1 cut(s) 160
CviJI RGCY 8 cut(s) 10, 77, 322, 337, 437, 514, 567, 630
CviKI_1 RGCY 8 cut(s) 10, 77, 322, 337, 437, 514, 567, 630
CviQI GTAC 3 cut(s) 224, 477, 589
DdeI CTNAG 3 cut(s) 110, 227, 546
DpnI GATC 2 cut(s) 420, 596
DpnII GATC 2 cut(s) 418, 594
Eco47I GGWCC 1 cut(s) 352
Eco88I CYCGRG 1 cut(s) 379
FaeI CATG 1 cut(s) 163
FaqI GGGAC 1 cut(s) 575
FatI CATG 1 cut(s) 159
FblI GTMKAC 1 cut(s) 390
Fnu4HI GCNGC 1 cut(s) 82
FokI GGATG 2 cut(s) 258, 558
Fsp4HI GCNGC 1 cut(s) 82
GluI GCNGC 1 cut(s) 82
GsaI CCCAGC 2 cut(s) 437, 630
HapII CCGG 1 cut(s) 78
Hin1II CATG 1 cut(s) 163
HinfI GANTC 4 cut(s) 64, 528, 542, 610
HpaII CCGG 1 cut(s) 78
Hpy166II GTNNAC 2 cut(s) 233, 391
Hpy188I TCNGA 4 cut(s) 63, 178, 245, 641
Hpy188III TCNNGA 2 cut(s) 381, 614
Hpy8I GTNNAC 2 cut(s) 233, 391
HpyAV CCTTC 1 cut(s) 153
HpyCH4III ACNGT 2 cut(s) 59, 504
HpyCH4IV ACGT 2 cut(s) 138, 266
HpyCH4V TGCA 4 cut(s) 233, 507, 524, 534
HpyF3I CTNAG 3 cut(s) 110, 227, 546
HpySE526I ACGT 2 cut(s) 138, 266
Hsp92II CATG 1 cut(s) 163
KroI GCCGGC 1 cut(s) 77
KroNI GCCGGC 1 cut(s) 79
Kzo9I GATC 2 cut(s) 418, 594
LmnI GCTCC 1 cut(s) 519
LpnPI CCDG 7 cut(s) 91, 383, 447, 465, 510, 524, 534
Lsp1109I GCAGC 1 cut(s) 93
LweI GCATC 1 cut(s) 323
MaeII ACGT 2 cut(s) 138, 266
MaeIII GTNAC 2 cut(s) 293, 493
MalI GATC 2 cut(s) 420, 596
MboI GATC 2 cut(s) 418, 594
MboII GAAGA 1 cut(s) 225
MhlI GDGCHC 2 cut(s) 211, 235
MluCI AATT 4 cut(s) 18, 125, 328, 619
MlyI GAGTC 2 cut(s) 536, 537
MmeI TCCRAC 3 cut(s) 4, 541, 599
MnlI CCTC 4 cut(s) 22, 184, 269, 641
MroNI GCCGGC 1 cut(s) 77
MseI TTAA 2 cut(s) 269, 360
MspA1I CMGCKG 1 cut(s) 322
MspI CCGG 1 cut(s) 78
NaeI GCCGGC 1 cut(s) 79
NdeII GATC 2 cut(s) 418, 594
NgoMIV GCCGGC 1 cut(s) 77
NlaIII CATG 1 cut(s) 163
NlaIV GGNNCC 1 cut(s) 484
NmuCI GTSAC 1 cut(s) 293
PaeR7I CTCGAG 1 cut(s) 379
PdiI GCCGGC 1 cut(s) 79
PfeI GAWTC 2 cut(s) 64, 610
PkrI GCNGC 1 cut(s) 83
PleI GAGTC 2 cut(s) 536, 536
PpsI GAGTC 2 cut(s) 536, 536
Psp1406I AACGTT 1 cut(s) 266
PspFI CCCAGC 2 cut(s) 433, 626
PspN4I GGNNCC 1 cut(s) 484
PspPI GGNCC 1 cut(s) 352
PstI CTGCAG 1 cut(s) 526
PvuII CAGCTG 1 cut(s) 322
RsaI GTAC 3 cut(s) 225, 478, 590
RsaNI GTAC 3 cut(s) 224, 477, 589
SaqAI TTAA 2 cut(s) 269, 360
SatI GCNGC 1 cut(s) 82
Sau3AI GATC 2 cut(s) 418, 594
Sau96I GGNCC 1 cut(s) 352
SbfI CCTGCAGG 1 cut(s) 526
SchI GAGTC 2 cut(s) 536, 537
SdaI CCTGCAGG 1 cut(s) 526
SduI GDGCHC 2 cut(s) 211, 235
SfaNI GCATC 1 cut(s) 323
SfcI CTRYAG 1 cut(s) 522
Sfr274I CTCGAG 1 cut(s) 379
SinI GGWCC 1 cut(s) 352
SlaI CTCGAG 1 cut(s) 379
SmlI CTYRAG 1 cut(s) 379
SmoI CTYRAG 1 cut(s) 379
Sse8387I CCTGCAGG 1 cut(s) 526
Sse9I AATT 4 cut(s) 18, 125, 328, 619
SsiI CCGC 2 cut(s) 114, 410
TaaI ACNGT 2 cut(s) 59, 504
TaiI ACGT 2 cut(s) 141, 269
TaqI TCGA 3 cut(s) 380, 417, 615
TasI AATT 4 cut(s) 18, 125, 328, 619
TfiI GAWTC 2 cut(s) 64, 610
Tru1I TTAA 2 cut(s) 269, 360
Tru9I TTAA 2 cut(s) 269, 360
TseFI GTSAC 1 cut(s) 293
TseI GCWGC 1 cut(s) 81
Tsp45I GTSAC 1 cut(s) 293
TspDTI ATGAA 2 cut(s) 34, 148
VneI GTGCAC 1 cut(s) 231
VpaK11BI GGWCC 1 cut(s) 352
XcmI CCANNNNNNNNNTGG 1 cut(s) 340
XhoI CTCGAG 1 cut(s) 379
XmiI GTMKAC 1 cut(s) 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.