AT1G75890

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001158
Physical Location & Seq
Forward (+)
28492944 .. 28495246
2303 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G75890.1

Sequence Viewer

Length: 1140 bp
ATGAAACGAAACAGTATAAATATTCATCACGTTACTTCTTTTTCTTCGAGTCCGTTTTGGTGTGTTTTCTTTCTCGTGTTGTTATGTAAGACGTCCACCAACGCTTTGGTGAAGCAGCCGCCAAACGAAACGACTCCGGCGATAATTGTGTTCGGAGATTCAATCGTTGATGCCGGAAATAATGACGACATTATGACAACGTTGGCTAGATGCAATTATCCTCCTTACGGCATCGATTTTGACGGTGGAATTCCTACCGGAAGATTTTGCAACGGCAAAGTTGCGACTGATTTTATAGCGGGAAAATTCGGAATTAAACCAAGTATACCGGCATATCGAAATCCAAATTTGAAACCAGAAGATCTCTTAACCGGTGTAACATTTGCGTCTGGTGGTGCTGGTTACGTTCCCTTCACAACCCAATTATCGACGTACCTTTTTATATATAAACCATTGTTGTTCTTAAAGGGAGGAATAGCTTTATCGCAGCAACTGAAGTTATTCGAAGAGTATGTAGAGAAGATGAAGAAAATGGTTGGGGAAGAAAGGACAAAGCTCATAATTAAAAACAGTTTGTTCATGGTTATATGTGGCAGTAATGATATAACCAACACCTACTTTGGTCTTCCTTCTGTTCAACAACAATACGACGTCGCTTCCTTTACCACTCTTATGGCCGACAATGCTCGATCTTTTGCTCAAAAATTACATGAATATGGAGCGAGGAGAATACAAGTATTCGGTGCACCACCAGTAGGATGTGTCCCATCACAGAGAACCTTAGCAGGAGGGCCAACAAGAAATTGTGTTGTTAGGTTCAATGATGCAACAAAGCTCTACAATGTTAAGCTCGCTGCAAATTTGGGTTCTTTGTCAAGAACTTTAGGAGACAAGACAATAATCTACGTTGACATATATGATTCTCTCCTTGATATCATTCTTGACCCTCGACAATATGGATTTAAGGTGGTTGATAAAGGATGTTGTGGAACTGGACTAATTGAAGTTGCTCTTTTATGCAACAACTTTGCAGCTGATGTATGTCCAAATAGAGATGAATATGTGTTTTGGGATAGTTTCCATCCGACTGAGAAGACTTACAGAATTATGGCCACAAAATATTTTGAGAGATATGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

379

Amino Acids

42.13

Weight (kDa)

8.64

Isoelectric Point (pI)

25.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 48 - 371 1.4e-36 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 95, 654
AccI GTMKAC 1 cut(s) 325
AciI CCGC 2 cut(s) 119, 299
AclI AACGTT 1 cut(s) 200
AcoI YGGCCR 2 cut(s) 675, 1110
AcsI RAATTY 4 cut(s) 249, 305, 346, 859
AcuI CTGAAG 1 cut(s) 515
AcyI GRCGYC 2 cut(s) 92, 651
AfaI GTAC 1 cut(s) 434
AfiI CCNNNNNNNGG 2 cut(s) 227, 755
AgeI ACCGGT 1 cut(s) 371
AgsI TTSAA 5 cut(s) 162, 352, 638, 820, 1004
AluBI AGCT 5 cut(s) 479, 556, 835, 850, 1034
AluI AGCT 5 cut(s) 479, 556, 835, 850, 1034
Alw21I GWGCWC 1 cut(s) 748
Alw26I GTCTC 1 cut(s) 882
Alw44I GTGCAC 1 cut(s) 744
AlwNI CAGNNNCTG 1 cut(s) 493
AoxI GGCC 3 cut(s) 675, 791, 1110
ApaLI GTGCAC 1 cut(s) 744
ApeKI GCWGC 4 cut(s) 115, 487, 854, 1031
ApoI RAATTY 4 cut(s) 249, 305, 346, 859
AsiGI ACCGGT 1 cut(s) 371
Asp700I GAANNNNTTC 1 cut(s) 500
AspS9I GGNCC 1 cut(s) 791
AsuHPI GGTGA 1 cut(s) 121
AsuII TTCGAA 1 cut(s) 504
BaeGI GKGCMC 1 cut(s) 748
BalI TGGCCA 1 cut(s) 1112
BauI CACGAG 1 cut(s) 74
BbsI GAAGAC 2 cut(s) 617, 1100
Bbv12I GWGCWC 1 cut(s) 748
BbvI GCAGC 4 cut(s) 127, 499, 841, 1043
BccI CCATC 2 cut(s) 775, 1089
BceAI ACGGC 2 cut(s) 244, 289
BcoDI GTCTC 1 cut(s) 882
BfaI CTAG 1 cut(s) 207
BglII AGATCT 1 cut(s) 361
BisI GCNGC 5 cut(s) 116, 119, 488, 855, 1032
BlsI GCNGC 5 cut(s) 117, 120, 489, 856, 1033
BmgT120I GGNCC 1 cut(s) 791
BmsI GCATC 4 cut(s) 160, 200, 240, 814
BpiI GAAGAC 2 cut(s) 617, 1100
Bpu10I CCTNAGC 1 cut(s) 781
Bpu14I TTCGAA 1 cut(s) 504
Bsa29I ATCGAT 1 cut(s) 234
BsaHI GRCGYC 2 cut(s) 92, 651
BsaWI WCCGGW 2 cut(s) 257, 371
Bsc4I CCNNNNNNNGG 2 cut(s) 227, 755
Bse118I RCCGGY 2 cut(s) 328, 371
Bse1I ACTGG 2 cut(s) 752, 997
BseCI ATCGAT 1 cut(s) 234
BseGI GGATG 3 cut(s) 764, 986, 1081
BseLI CCNNNNNNNGG 2 cut(s) 227, 755
BseMII CTCAG 1 cut(s) 1080
BseNI ACTGG 2 cut(s) 752, 997
BseRI GAGGAG 1 cut(s) 739
BseSI GKGCMC 1 cut(s) 748
BseXI GCAGC 4 cut(s) 127, 499, 841, 1043
BshFI GGCC 3 cut(s) 677, 793, 1112
BshTI ACCGGT 1 cut(s) 371
BshVI ATCGAT 1 cut(s) 234
BsiHKAI GWGCWC 1 cut(s) 748
BsiSI CCGG 5 cut(s) 137, 174, 258, 329, 372
BslFI GGGAC 1 cut(s) 749
BslI CCNNNNNNNGG 2 cut(s) 227, 755
BsmAI GTCTC 1 cut(s) 882
BsmFI GGGAC 1 cut(s) 749
BsnI GGCC 3 cut(s) 677, 793, 1112
Bsp119I TTCGAA 1 cut(s) 504
Bsp1286I GDGCHC 1 cut(s) 748
Bsp143I GATC 2 cut(s) 361, 689
BspACI CCGC 2 cut(s) 119, 299
BspANI GGCC 3 cut(s) 677, 793, 1112
BspCNI CTCAG 1 cut(s) 1081
BspDI ATCGAT 1 cut(s) 234
BspT104I TTCGAA 1 cut(s) 504
BsrFI RCCGGY 2 cut(s) 328, 371
BsrI ACTGG 2 cut(s) 752, 997
BssAI RCCGGY 2 cut(s) 328, 371
BssMI GATC 2 cut(s) 361, 689
BssNAI GTATAC 1 cut(s) 326
BssNI GRCGYC 2 cut(s) 92, 651
BssSI CACGAG 1 cut(s) 74
Bst1107I GTATAC 1 cut(s) 326
Bst2BI CACGAG 1 cut(s) 74
Bst4CI ACNGT 3 cut(s) 14, 245, 572
Bst6I CTCTTC 1 cut(s) 501
BstACI GRCGYC 2 cut(s) 92, 651
BstBI TTCGAA 1 cut(s) 504
BstC8I GCNNGC 1 cut(s) 852
BstDEI CTNAG 2 cut(s) 781, 1089
BstF5I GGATG 3 cut(s) 764, 986, 1081
BstKTI GATC 2 cut(s) 364, 692
BstMAI GTCTC 1 cut(s) 882
BstMBI GATC 2 cut(s) 361, 689
BstMWI GCNNNNNNNGC 1 cut(s) 683
BstSLI GKGCMC 1 cut(s) 748
BstV1I GCAGC 4 cut(s) 127, 499, 841, 1043
BstV2I GAAGAC 2 cut(s) 617, 1100
BstX2I RGATCY 1 cut(s) 361
BstXI CCANNNNNNTGG 2 cut(s) 106, 673
BstYI RGATCY 1 cut(s) 361
BstZ17I GTATAC 1 cut(s) 326
Bsu15I ATCGAT 1 cut(s) 234
BsuRI GGCC 3 cut(s) 677, 793, 1112
BsuTUI ATCGAT 1 cut(s) 234
BtsCI GGATG 3 cut(s) 764, 986, 1081
Cac8I GCNNGC 1 cut(s) 852
CaiI CAGNNNCTG 1 cut(s) 493
Cfr10I RCCGGY 2 cut(s) 328, 371
Cfr13I GGNCC 1 cut(s) 791
ClaI ATCGAT 1 cut(s) 234
CseI GACGC 1 cut(s) 375
Csp6I GTAC 1 cut(s) 433
CspAI ACCGGT 1 cut(s) 371
CviAII CATG 2 cut(s) 580, 710
CviQI GTAC 1 cut(s) 433
DdeI CTNAG 2 cut(s) 781, 1089
DpnI GATC 2 cut(s) 363, 691
DpnII GATC 2 cut(s) 361, 689
EaeI YGGCCR 2 cut(s) 675, 1110
Eam1104I CTCTTC 1 cut(s) 501
EarI CTCTTC 1 cut(s) 501
Eco32I GATATC 1 cut(s) 934
Eco57I CTGAAG 1 cut(s) 515
EcoRI GAATTC 1 cut(s) 249
EcoRV GATATC 1 cut(s) 934
FaeI CATG 2 cut(s) 583, 713
FalI AAGNNNNNCTT 2 cut(s) 996, 1028
FaqI GGGAC 1 cut(s) 749
FatI CATG 2 cut(s) 579, 709
FauI CCCGC 1 cut(s) 292
FblI GTMKAC 1 cut(s) 325
Fnu4HI GCNGC 5 cut(s) 116, 119, 488, 855, 1032
FokI GGATG 3 cut(s) 771, 993, 1068
Fsp4HI GCNGC 5 cut(s) 116, 119, 488, 855, 1032
FspBI CTAG 1 cut(s) 207
GluI GCNGC 5 cut(s) 116, 119, 488, 855, 1032
HaeIII GGCC 3 cut(s) 677, 793, 1112
HapII CCGG 5 cut(s) 137, 174, 258, 329, 372
HgaI GACGC 1 cut(s) 375
Hin1I GRCGYC 2 cut(s) 92, 651
Hin1II CATG 2 cut(s) 583, 713
HincII GTYRAC 1 cut(s) 910
HindII GTYRAC 1 cut(s) 910
HinfI GANTC 4 cut(s) 49, 133, 158, 920
HpaII CCGG 5 cut(s) 137, 174, 258, 329, 372
HphI GGTGA 1 cut(s) 121
Hpy166II GTNNAC 4 cut(s) 96, 326, 746, 910
Hpy188I TCNGA 3 cut(s) 155, 311, 1086
Hpy188III TCNNGA 2 cut(s) 876, 941
Hpy8I GTNNAC 4 cut(s) 96, 326, 746, 910
Hpy99I CGWCG 3 cut(s) 433, 653, 656
HpyAV CCTTC 2 cut(s) 421, 639
HpyCH4III ACNGT 3 cut(s) 14, 245, 572
HpyCH4IV ACGT 7 cut(s) 30, 92, 200, 405, 431, 651, 906
HpyCH4V TGCA 7 cut(s) 213, 270, 746, 827, 857, 1020, 1031
HpyF10VI GCNNNNNNNGC 1 cut(s) 683
HpyF3I CTNAG 2 cut(s) 781, 1089
HpySE526I ACGT 7 cut(s) 30, 92, 200, 405, 431, 651, 906
Hsp92I GRCGYC 2 cut(s) 92, 651
Hsp92II CATG 2 cut(s) 583, 713
Kzo9I GATC 2 cut(s) 361, 689
LmnI GCTCC 1 cut(s) 719
Lsp1109I GCAGC 4 cut(s) 127, 499, 841, 1043
LweI GCATC 4 cut(s) 160, 200, 240, 814
MaeI CTAG 1 cut(s) 207
MaeII ACGT 7 cut(s) 30, 92, 200, 405, 431, 651, 906
MaeIII GTNAC 3 cut(s) 31, 376, 401
MalI GATC 2 cut(s) 363, 691
MboI GATC 2 cut(s) 361, 689
MboII GAAGA 9 cut(s) 36, 273, 371, 518, 532, 538, 554, 617, 1105
MflI RGATCY 1 cut(s) 361
MhlI GDGCHC 1 cut(s) 748
MlsI TGGCCA 1 cut(s) 1112
MluNI TGGCCA 1 cut(s) 1112
MlyI GAGTC 2 cut(s) 58, 127
MmeI TCCRAC 1 cut(s) 1109
MnlI CCTC 5 cut(s) 231, 464, 717, 782, 957
Mox20I TGGCCA 1 cut(s) 1112
MroXI GAANNNNTTC 1 cut(s) 500
MscI TGGCCA 1 cut(s) 1112
MseI TTAA 6 cut(s) 315, 368, 464, 564, 846, 963
MslI CAYNNNNRTG 2 cut(s) 671, 714
Msp20I TGGCCA 1 cut(s) 1112
MspA1I CMGCKG 1 cut(s) 1034
MspI CCGG 5 cut(s) 137, 174, 258, 329, 372
MwoI GCNNNNNNNGC 1 cut(s) 683
NdeII GATC 2 cut(s) 361, 689
NlaIII CATG 2 cut(s) 583, 713
NspV TTCGAA 1 cut(s) 504
PcsI WCGNNNNNNNCGW 1 cut(s) 137
PdmI GAANNNNTTC 1 cut(s) 500
PfeI GAWTC 2 cut(s) 158, 920
PinAI ACCGGT 1 cut(s) 371
PkrI GCNGC 5 cut(s) 117, 120, 489, 856, 1033
PleI GAGTC 2 cut(s) 57, 127
PpsI GAGTC 2 cut(s) 57, 127
Psp1406I AACGTT 1 cut(s) 200
PspPI GGNCC 1 cut(s) 791
PstNI CAGNNNCTG 1 cut(s) 493
PsuI RGATCY 1 cut(s) 361
PvuII CAGCTG 1 cut(s) 1034
RsaI GTAC 1 cut(s) 434
RsaNI GTAC 1 cut(s) 433
RseI CAYNNNNRTG 2 cut(s) 671, 714
SaqAI TTAA 6 cut(s) 315, 368, 464, 564, 846, 963
SatI GCNGC 5 cut(s) 116, 119, 488, 855, 1032
Sau3AI GATC 2 cut(s) 361, 689
Sau96I GGNCC 1 cut(s) 791
SchI GAGTC 2 cut(s) 58, 127
SduI GDGCHC 1 cut(s) 748
SfaNI GCATC 4 cut(s) 160, 200, 240, 814
SfuI TTCGAA 1 cut(s) 504
SmiMI CAYNNNNRTG 2 cut(s) 671, 714
SsiI CCGC 2 cut(s) 119, 299
SspI AATATT 2 cut(s) 22, 1121
SspMI CTAG 1 cut(s) 207
TaaI ACNGT 3 cut(s) 14, 245, 572
TaiI ACGT 7 cut(s) 33, 95, 203, 408, 434, 654, 909
TaqI TCGA 7 cut(s) 47, 234, 337, 428, 504, 688, 949
TauI GCSGC 1 cut(s) 121
TfiI GAWTC 2 cut(s) 158, 920
Tru1I TTAA 6 cut(s) 315, 368, 464, 564, 846, 963
Tru9I TTAA 6 cut(s) 315, 368, 464, 564, 846, 963
TseI GCWGC 4 cut(s) 115, 487, 854, 1031
TspDTI ATGAA 6 cut(s) 14, 17, 539, 568, 726, 1071
TspGWI ACGGA 1 cut(s) 42
VneI GTGCAC 1 cut(s) 744
XapI RAATTY 4 cut(s) 249, 305, 346, 859
XcmI CCANNNNNNNNNTGG 1 cut(s) 103
XmiI GTMKAC 1 cut(s) 325
XmnI GAANNNNTTC 1 cut(s) 500
XspI CTAG 1 cut(s) 207
ZraI GACGTC 2 cut(s) 93, 652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.