RchiOBHm_Chr6g0303381

GDSL-like Lipase/Acylhydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0001158
Physical Location & Seq
Forward (+)
63117612 .. 63119786
2175 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27254

Sequence Viewer

Length: 1212 bp
ATGAGCTTAAAAACCAACATTTGTCACTTATCCTCTTGTATGCTTCCTCTTTGGACCGGAGGAAATTCCAAAAGAATGGGATTCCTCTTTATAGAAACCTTCTCTTCTTCTTCTTCCTCTGGTTCCATAATGTCGTCTTCGAAGGAGCTCTTGATATTTGTTTGTAGTCTTGCTGTGGTTTTAGGGCAGTACCCCAGAATCACGGAGGGTCGGGTGAATATACCACCAGGAAAGTCAGTGCCGGCGGTTATAGTGTTCGGTGATTCGATCATGGACACCGGCAACAATAACAATCTTAACTCTGCTGTTAAATGCAATTTCCCTCCATATGGTGAAAATTTCCCAGGAAAAGTTCCAACCGGTAGATTTGGCAATGGAAAAGTCCCCTCAGACTTCATAGCTCAAGAACTGGGAATCAAAGAGTTTGTGCCGGCATATGCGGATACTAGTCTAAAACCTGAAGATCTCAGCACTGGAGTCTGCTTCGCTTCAGGTGGCACCGGATATGACCCTCAGACCCCCGAATTAGTAGCAGTTATATCGCTATCGGAGCAGTTGAACATGTTCAAGGAGTATATAGGGAAGCTGAAAGCCAATTTTGGCGAGCAGAGAACAAGCTTCATCCTCGCCAACGGTCTCTTTCTGGTGGTCGCCGGTAGCGATGACATTGCCAACACCTATTTCACTATTGGCATCAGAAAGGCAGAATATGACGTCCCTGCTTACACTGACCTTATGGTCAACTCTGCCACTAGTTTCCTTCAGGATTTGTATGCACTTGGGGCTAGAAGGTTCGGTGTTTTCAGTGCACCACCGATAGGGTGTGTACCTTCACAAAGAACTCTAGGTGGAGGAATACAAAGACAGTGTGCAGAAGAGTACAACGACGCAGCGAAGCTATTCAACTCTAAGCTGTCGTCCAACATGCAGTCCTTGAATACAAAGCTGCCCGACAGTAGAATGGTTTATATCGATGTTTACACCCCTCTACTTGATGTCATTCTTCACCCTGCCAAATACGGGTTTAAAATGGCGGAGAAAGGTTGCTGTGGCACAGGGGCACTAGAGGTAGCGATATTATGCAACAAATTGTCTCCAACGTGTGAAAATCCGGCGGATCATGTATTTTGGGATAGTTATCACCCTACTGAACAAGCATACAGAGTACTCATTCCTCCAGTTCTCCAAAAATACGTAAACCAATTCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

403

Amino Acids

43.85

Weight (kDa)

6.14

Isoelectric Point (pI)

40.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 83 - 390 3.6e-31 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 737
AatII GACGTC 1 cut(s) 717
AccB1I GGYRCC 1 cut(s) 497
AciI CCGC 4 cut(s) 245, 440, 1034, 1115
AclWI GGATC 1 cut(s) 1125
AcsI RAATTY 2 cut(s) 64, 337
AcuI CTGAAG 3 cut(s) 474, 480, 746
AcyI GRCGYC 1 cut(s) 714
AfaI GTAC 4 cut(s) 191, 828, 881, 1167
AfiI CCNNNNNNNGG 3 cut(s) 329, 818, 1020
AflIII ACRYGT 2 cut(s) 561, 1100
AgeI ACCGGT 1 cut(s) 359
AgsI TTSAA 4 cut(s) 559, 568, 904, 937
AhlI ACTAGT 2 cut(s) 446, 752
AjnI CCWGG 2 cut(s) 226, 343
AjuI GAANNNNNNNTTGG 2 cut(s) 665, 697
AluBI AGCT 8 cut(s) 6, 148, 401, 586, 618, 898, 913, 946
AluI AGCT 8 cut(s) 6, 148, 401, 586, 618, 898, 913, 946
Alw21I GWGCWC 2 cut(s) 150, 811
Alw26I GTCTC 2 cut(s) 641, 1098
Alw44I GTGCAC 1 cut(s) 807
AlwI GGATC 1 cut(s) 1125
ApaLI GTGCAC 1 cut(s) 807
ApeKI GCWGC 2 cut(s) 890, 946
ApoI RAATTY 2 cut(s) 64, 337
AsiGI ACCGGT 1 cut(s) 359
Asp700I GAANNNNTTC 2 cut(s) 563, 899
AspS9I GGNCC 1 cut(s) 54
AsuHPI GGTGA 5 cut(s) 226, 272, 344, 998, 1133
AsuII TTCGAA 1 cut(s) 140
AvaII GGWCC 1 cut(s) 54
BaeGI GKGCMC 2 cut(s) 811, 1063
BanI GGYRCC 1 cut(s) 497
BanII GRGCYC 1 cut(s) 150
BbsI GAAGAC 1 cut(s) 129
Bbv12I GWGCWC 2 cut(s) 150, 811
BbvI GCAGC 2 cut(s) 902, 933
BcgI CGANNNNNNTGC 4 cut(s) 522, 556, 650, 684
BciT130I CCWGG 2 cut(s) 228, 345
BciVI GTATCC 1 cut(s) 436
BcoDI GTCTC 2 cut(s) 641, 1098
BcuI ACTAGT 2 cut(s) 446, 752
BfaI CTAG 5 cut(s) 447, 753, 786, 845, 1064
BfuI GTATCC 1 cut(s) 436
BglII AGATCT 1 cut(s) 463
BisI GCNGC 2 cut(s) 891, 947
BlsI GCNGC 2 cut(s) 892, 948
BmcAI AGTACT 1 cut(s) 1167
Bme1390I CCNGG 2 cut(s) 228, 345
Bme18I GGWCC 1 cut(s) 54
BmgT120I GGNCC 1 cut(s) 54
BmiI GGNNCC 2 cut(s) 124, 499
BmrFI CCNGG 2 cut(s) 228, 345
BmrI ACTGGG 1 cut(s) 419
BmsI GCATC 1 cut(s) 702
BmuI ACTGGG 1 cut(s) 419
BpiI GAAGAC 1 cut(s) 129
BpmI CTGGAG 2 cut(s) 495, 1161
Bpu14I TTCGAA 1 cut(s) 140
BpuEI CTTGAG 1 cut(s) 387
Bsa29I ATCGAT 1 cut(s) 972
BsaAI YACGTR 1 cut(s) 1195
BsaBI GATNNNNATC 1 cut(s) 1137
BsaHI GRCGYC 1 cut(s) 714
BsaI GGTCTC 1 cut(s) 641
BsaJI CCNNGG 1 cut(s) 343
BsaWI WCCGGW 3 cut(s) 56, 359, 500
Bsc4I CCNNNNNNNGG 3 cut(s) 329, 818, 1020
Bse118I RCCGGY 5 cut(s) 241, 278, 359, 430, 653
Bse1I ACTGG 3 cut(s) 414, 478, 1178
Bse3DI GCAATG 2 cut(s) 379, 666
Bse8I GATNNNNATC 1 cut(s) 1137
BseBI CCWGG 2 cut(s) 228, 345
BseCI ATCGAT 1 cut(s) 972
BseDI CCNNGG 1 cut(s) 343
BseGI GGATG 1 cut(s) 621
BseJI GATNNNNATC 1 cut(s) 1137
BseLI CCNNNNNNNGG 3 cut(s) 329, 818, 1020
BseMI GCAATG 2 cut(s) 379, 666
BseMII CTCAG 3 cut(s) 402, 481, 527
BseNI ACTGG 3 cut(s) 414, 478, 1178
BseSI GKGCMC 2 cut(s) 811, 1063
BseXI GCAGC 2 cut(s) 902, 933
BsgI GTGCAG 1 cut(s) 891
BshNI GGYRCC 1 cut(s) 497
BshTI ACCGGT 1 cut(s) 359
BshVI ATCGAT 1 cut(s) 972
BsiHKAI GWGCWC 2 cut(s) 150, 811
BsiSI CCGG 8 cut(s) 57, 242, 279, 360, 431, 501, 654, 1112
BslFI GGGAC 2 cut(s) 368, 701
BslI CCNNNNNNNGG 3 cut(s) 329, 818, 1020
BsmAI GTCTC 2 cut(s) 641, 1098
BsmFI GGGAC 2 cut(s) 368, 701
Bso31I GGTCTC 1 cut(s) 641
Bsp119I TTCGAA 1 cut(s) 140
Bsp1286I GDGCHC 3 cut(s) 150, 811, 1063
Bsp143I GATC 3 cut(s) 267, 463, 1117
BspACI CCGC 4 cut(s) 245, 440, 1034, 1115
BspCNI CTCAG 3 cut(s) 401, 480, 526
BspDI ATCGAT 1 cut(s) 972
BspLI GGNNCC 2 cut(s) 124, 499
BspPI GGATC 1 cut(s) 1125
BspT104I TTCGAA 1 cut(s) 140
BspT107I GGYRCC 1 cut(s) 497
BspTNI GGTCTC 1 cut(s) 641
BsrDI GCAATG 2 cut(s) 379, 666
BsrFI RCCGGY 5 cut(s) 241, 278, 359, 430, 653
BsrI ACTGG 3 cut(s) 414, 478, 1178
BssAI RCCGGY 5 cut(s) 241, 278, 359, 430, 653
BssECI CCNNGG 1 cut(s) 343
BssMI GATC 3 cut(s) 267, 463, 1117
BssNI GRCGYC 1 cut(s) 714
Bst2UI CCWGG 2 cut(s) 228, 345
Bst4CI ACNGT 3 cut(s) 635, 867, 956
Bst6I CTCTTC 2 cut(s) 109, 870
BstACI GRCGYC 1 cut(s) 714
BstBAI YACGTR 1 cut(s) 1195
BstBI TTCGAA 1 cut(s) 140
BstC8I GCNNGC 3 cut(s) 243, 432, 605
BstDEI CTNAG 4 cut(s) 388, 467, 513, 909
BstF5I GGATG 1 cut(s) 621
BstKTI GATC 3 cut(s) 270, 466, 1120
BstMAI GTCTC 2 cut(s) 641, 1098
BstMBI GATC 3 cut(s) 267, 463, 1117
BstMWI GCNNNNNNNGC 2 cut(s) 550, 782
BstNI CCWGG 2 cut(s) 228, 345
BstNSI RCATGY 2 cut(s) 565, 928
BstSCI CCNGG 2 cut(s) 226, 343
BstSLI GKGCMC 2 cut(s) 811, 1063
BstSNI TACGTA 1 cut(s) 1195
BstV1I GCAGC 2 cut(s) 902, 933
BstV2I GAAGAC 1 cut(s) 129
BstX2I RGATCY 1 cut(s) 463
BstXI CCANNNNNNTGG 1 cut(s) 76
BstYI RGATCY 1 cut(s) 463
Bsu15I ATCGAT 1 cut(s) 972
BsuI GTATCC 1 cut(s) 436
BsuTUI ATCGAT 1 cut(s) 972
BtgZI GCGATG 1 cut(s) 675
BtsCI GGATG 1 cut(s) 621
BtsIMutI CAGTG 5 cut(s) 243, 471, 726, 811, 872
Cac8I GCNNGC 3 cut(s) 243, 432, 605
Cfr10I RCCGGY 5 cut(s) 241, 278, 359, 430, 653
Cfr13I GGNCC 1 cut(s) 54
ClaI ATCGAT 1 cut(s) 972
CseI GACGC 1 cut(s) 896
Csp6I GTAC 4 cut(s) 190, 827, 880, 1166
CspAI ACCGGT 1 cut(s) 359
CviAII CATG 4 cut(s) 271, 562, 925, 1121
CviQI GTAC 4 cut(s) 190, 827, 880, 1166
DdeI CTNAG 4 cut(s) 388, 467, 513, 909
DpnI GATC 3 cut(s) 269, 465, 1119
DpnII GATC 3 cut(s) 267, 463, 1117
DraI TTTAAA 1 cut(s) 1027
DrdI GACNNNNNNGTC 1 cut(s) 737
DseDI GACNNNNNNGTC 1 cut(s) 737
Eam1104I CTCTTC 2 cut(s) 109, 870
EarI CTCTTC 2 cut(s) 109, 870
EciI GGCGGA 2 cut(s) 1049, 1130
Ecl136II GAGCTC 1 cut(s) 148
Eco105I TACGTA 1 cut(s) 1195
Eco24I GRGCYC 1 cut(s) 150
Eco31I GGTCTC 1 cut(s) 641
Eco47I GGWCC 1 cut(s) 54
Eco53kI GAGCTC 1 cut(s) 148
Eco57I CTGAAG 3 cut(s) 474, 480, 746
EcoICRI GAGCTC 1 cut(s) 148
EcoRII CCWGG 2 cut(s) 226, 343
EcoT38I GRGCYC 1 cut(s) 150
FaeI CATG 4 cut(s) 274, 565, 928, 1124
FalI AAGNNNNNCTT 2 cut(s) 134, 166
FaqI GGGAC 2 cut(s) 368, 701
FatI CATG 4 cut(s) 270, 561, 924, 1120
FauNDI CATATG 2 cut(s) 328, 436
Fnu4HI GCNGC 2 cut(s) 891, 947
FokI GGATG 1 cut(s) 608
FriOI GRGCYC 1 cut(s) 150
Fsp4HI GCNGC 2 cut(s) 891, 947
FspBI CTAG 5 cut(s) 447, 753, 786, 845, 1064
GluI GCNGC 2 cut(s) 891, 947
GsuI CTGGAG 2 cut(s) 495, 1161
HapII CCGG 8 cut(s) 57, 242, 279, 360, 431, 501, 654, 1112
HgaI GACGC 1 cut(s) 896
Hin1I GRCGYC 1 cut(s) 714
Hin1II CATG 4 cut(s) 274, 565, 928, 1124
HincII GTYRAC 1 cut(s) 742
HindII GTYRAC 1 cut(s) 742
HindIII AAGCTT 1 cut(s) 616
HinfI GANTC 5 cut(s) 81, 198, 263, 414, 477
HpaII CCGG 8 cut(s) 57, 242, 279, 360, 431, 501, 654, 1112
HphI GGTGA 5 cut(s) 226, 272, 344, 998, 1133
Hpy166II GTNNAC 5 cut(s) 742, 809, 827, 979, 1198
Hpy188I TCNGA 4 cut(s) 391, 516, 550, 698
Hpy188III TCNNGA 3 cut(s) 151, 404, 764
Hpy8I GTNNAC 5 cut(s) 742, 809, 827, 979, 1198
Hpy99I CGWCG 1 cut(s) 890
HpyAV CCTTC 5 cut(s) 109, 136, 770, 783, 840
HpyCH4III ACNGT 3 cut(s) 635, 867, 956
HpyCH4IV ACGT 3 cut(s) 714, 1100, 1194
HpyCH4V TGCA 6 cut(s) 315, 776, 809, 872, 928, 1083
HpyF10VI GCNNNNNNNGC 2 cut(s) 550, 782
HpyF3I CTNAG 4 cut(s) 388, 467, 513, 909
HpySE526I ACGT 3 cut(s) 714, 1100, 1194
Hsp92I GRCGYC 1 cut(s) 714
Hsp92II CATG 4 cut(s) 274, 565, 928, 1124
KroI GCCGGC 2 cut(s) 241, 430
KroNI GCCGGC 2 cut(s) 243, 432
Kzo9I GATC 3 cut(s) 267, 463, 1117
LmnI GCTCC 2 cut(s) 145, 550
Lsp1109I GCAGC 2 cut(s) 902, 933
LweI GCATC 1 cut(s) 702
MaeI CTAG 5 cut(s) 447, 753, 786, 845, 1064
MaeII ACGT 3 cut(s) 714, 1100, 1194
MaeIII GTNAC 1 cut(s) 23
MalI GATC 3 cut(s) 269, 465, 1119
MboI GATC 3 cut(s) 267, 463, 1117
MboII GAAGA 8 cut(s) 96, 99, 102, 105, 129, 473, 887, 995
MflI RGATCY 1 cut(s) 463
MhlI GDGCHC 3 cut(s) 150, 811, 1063
MluCI AATT 7 cut(s) 64, 316, 337, 524, 595, 1088, 1202
MlyI GAGTC 1 cut(s) 486
MmeI TCCRAC 3 cut(s) 380, 945, 1121
MroNI GCCGGC 2 cut(s) 241, 430
MroXI GAANNNNTTC 2 cut(s) 563, 899
MseI TTAA 4 cut(s) 8, 297, 309, 1026
MspI CCGG 8 cut(s) 57, 242, 279, 360, 431, 501, 654, 1112
MspR9I CCNGG 2 cut(s) 228, 345
MvaI CCWGG 2 cut(s) 228, 345
MwoI GCNNNNNNNGC 2 cut(s) 550, 782
NaeI GCCGGC 2 cut(s) 243, 432
NdeI CATATG 2 cut(s) 328, 436
NdeII GATC 3 cut(s) 267, 463, 1117
NgoMIV GCCGGC 2 cut(s) 241, 430
NlaIII CATG 4 cut(s) 274, 565, 928, 1124
NlaIV GGNNCC 2 cut(s) 124, 499
NmuCI GTSAC 1 cut(s) 23
NspI RCATGY 2 cut(s) 565, 928
NspV TTCGAA 1 cut(s) 140
PciI ACATGT 1 cut(s) 561
PcsI WCGNNNNNNNCGW 1 cut(s) 657
PdiI GCCGGC 2 cut(s) 243, 432
PdmI GAANNNNTTC 2 cut(s) 563, 899
PfeI GAWTC 4 cut(s) 81, 198, 263, 414
PinAI ACCGGT 1 cut(s) 359
PkrI GCNGC 2 cut(s) 892, 948
PleI GAGTC 1 cut(s) 485
PpsI GAGTC 1 cut(s) 485
Ppu21I YACGTR 1 cut(s) 1195
PscI ACATGT 1 cut(s) 561
Psp124BI GAGCTC 1 cut(s) 150
Psp6I CCWGG 2 cut(s) 226, 343
PspGI CCWGG 2 cut(s) 226, 343
PspN4I GGNNCC 2 cut(s) 124, 499
PspPI GGNCC 1 cut(s) 54
PsuI RGATCY 1 cut(s) 463
RsaI GTAC 4 cut(s) 191, 828, 881, 1167
RsaNI GTAC 4 cut(s) 190, 827, 880, 1166
SacI GAGCTC 1 cut(s) 150
SaqAI TTAA 4 cut(s) 8, 297, 309, 1026
SatI GCNGC 2 cut(s) 891, 947
Sau3AI GATC 3 cut(s) 267, 463, 1117
Sau96I GGNCC 1 cut(s) 54
ScaI AGTACT 1 cut(s) 1167
SchI GAGTC 1 cut(s) 486
ScrFI CCNGG 2 cut(s) 228, 345
SduI GDGCHC 3 cut(s) 150, 811, 1063
SfaNI GCATC 1 cut(s) 702
SfuI TTCGAA 1 cut(s) 140
SinI GGWCC 1 cut(s) 54
SmlI CTYRAG 1 cut(s) 402
SmoI CTYRAG 1 cut(s) 402
SnaBI TACGTA 1 cut(s) 1195
SpeI ACTAGT 2 cut(s) 446, 752
Sse9I AATT 7 cut(s) 64, 316, 337, 524, 595, 1088, 1202
SsiI CCGC 4 cut(s) 245, 440, 1034, 1115
SspMI CTAG 5 cut(s) 447, 753, 786, 845, 1064
SstI GAGCTC 1 cut(s) 150
StyD4I CCNGG 2 cut(s) 226, 343
TaaI ACNGT 3 cut(s) 635, 867, 956
TaiI ACGT 3 cut(s) 717, 1103, 1197
TaqI TCGA 3 cut(s) 140, 266, 972
TasI AATT 7 cut(s) 64, 316, 337, 524, 595, 1088, 1202
TatI WGTACW 2 cut(s) 879, 1165
TfiI GAWTC 4 cut(s) 81, 198, 263, 414
Tru1I TTAA 4 cut(s) 8, 297, 309, 1026
Tru9I TTAA 4 cut(s) 8, 297, 309, 1026
TscAI CASTG 5 cut(s) 243, 478, 733, 811, 872
TseFI GTSAC 1 cut(s) 23
TseI GCWGC 2 cut(s) 890, 946
Tsp45I GTSAC 1 cut(s) 23
TspDTI ATGAA 2 cut(s) 385, 610
TspGWI ACGGA 1 cut(s) 218
TspRI CASTG 5 cut(s) 243, 478, 733, 811, 872
VneI GTGCAC 1 cut(s) 807
VpaK11BI GGWCC 1 cut(s) 54
XapI RAATTY 2 cut(s) 64, 337
XceI RCATGY 2 cut(s) 565, 928
XmnI GAANNNNTTC 2 cut(s) 563, 899
XspI CTAG 5 cut(s) 447, 753, 786, 845, 1064
ZraI GACGTC 1 cut(s) 715
ZrmI AGTACT 1 cut(s) 1167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.