FvH4_2g38120

GDSL-like Lipase/Acylhydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001158
Physical Location & Seq
Reverse (-)
27618316 .. 27620651
2336 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g38120.t1

Sequence Viewer

Length: 1134 bp
ATGGGGTTCCTCTCTTCTTCTTCTTCATCATCTTCTTCCTCTGGTGCCTTGATGTCTTCTTCGAAGAAGCTCTTGATCTTTGTTTGTAGTCTTGCTATGGTTTTAGGGCACTACCCCAGAATCACAGAGTGTCGGGTGCAGATACCACCGGGAAAGACATTGCCGGCGATTATAATTTTCGGTGATTCGATCATGGACACCGGCAACAACAACAATCTTAAGTCAGTTATCAAATGCAATTTCCCTCCTTATGGTCAAAATTTCCCTGGAAAAGTTCCAACTGGTAGATTTGGCAATGGAAAGGTTCCCTCAGACTTCATAGCTCATGAATTGGGAGTCAAAGAGTTTGTGCCGCCATATGCGGATACTAGTCTAACTCCTGAAGAACTCAGCACCGGAGTCTGCTTTGCTTCAGGTGGCACCGGATATGACCCTCAGACACCCGAGTTAGTATCAGTTATATCGCTATCGGATCAGTTGAACAGCTTCAAAGAGTATATAGGGAAGCTGAAAGCTAATTTTGGCGAGGAGAGAACAAATTTCATCCTCGCCAACAGTCTCTTTCTGGTGGTCGCCGGTAGCGATGACATTGCCAACACCTATTTCACTATCGGCATCAGAAAGGCACAATACGACGTCCCTGCTTACACTGACCTTATGGTCAACTCCGCCACTAATTTCCTTCAGGATCTGTATGCACTTGGGGCTAGAAGGTTTGGTGTTTTCAGTGCACCACCAATAGGGTGTGTGCCTTCACAGAGAACTCTAGGTGGAGGAATTCAAAGACAGTGTGCAGAAGAATACAACGACGCAGCGAAGCTATTCAACTCTAAGCTGTCGGCAAACATGAAGTCCTTAAATTCAAAGCTGCCCGATAGTAGAATGGTTTATATCGATGTTTACACTCCTATACTTGATATCATTCTATACCCTACGAAATACGGGCTTAAAGTGGCGAATAAAGGTTGCTGCGGCACAGGGGTACTAGAGGTGGCGATATTATGCACCAAATTGTCTCCGACGTGTGAAAATCCAGGGGATCATGTGTTCTGGGATAGTTATCACCCTACTGAACAAGCATACAGTGTGCTCATTCCTCCACTTCTCCAGAAGTACGTAAACCACTTCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

378

Amino Acids

40.96

Weight (kDa)

6.93

Isoelectric Point (pI)

34.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 57 - 364 7.2e-32 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 173
AasI GACNNNNNNGTC 1 cut(s) 659
AatII GACGTC 1 cut(s) 639
AccB1I GGYRCC 2 cut(s) 44, 419
AciI CCGC 4 cut(s) 353, 362, 669, 972
AclWI GGATC 3 cut(s) 480, 696, 1047
AcsI RAATTY 4 cut(s) 259, 538, 777, 859
AcuI CTGAAG 3 cut(s) 396, 402, 668
AcyI GRCGYC 1 cut(s) 636
AdeI CACNNNGTG 1 cut(s) 129
AfaI GTAC 2 cut(s) 984, 1115
AfiI CCNNNNNNNGG 2 cut(s) 251, 740
AflII CTTAAG 1 cut(s) 218
AflIII ACRYGT 1 cut(s) 1022
AgsI TTSAA 5 cut(s) 481, 490, 782, 826, 864
AhlI ACTAGT 1 cut(s) 368
AjiI CACGTC 1 cut(s) 1023
AjnI CCWGG 2 cut(s) 265, 1033
AjuI GAANNNNNNNTTGG 2 cut(s) 587, 619
AloI GAACNNNNNNTCC 2 cut(s) 1031, 1063
AluBI AGCT 8 cut(s) 70, 323, 486, 508, 515, 820, 835, 868
AluI AGCT 8 cut(s) 70, 323, 486, 508, 515, 820, 835, 868
Alw21I GWGCWC 2 cut(s) 733, 1092
Alw26I GTCTC 2 cut(s) 563, 1020
Alw44I GTGCAC 1 cut(s) 729
AlwI GGATC 3 cut(s) 480, 696, 1047
AlwNI CAGNNNCTG 1 cut(s) 691
Ama87I CYCGRG 1 cut(s) 443
ApaLI GTGCAC 1 cut(s) 729
ApeKI GCWGC 3 cut(s) 812, 868, 969
ApoI RAATTY 4 cut(s) 259, 538, 777, 859
ArsI GACNNNNNNTTYG 2 cut(s) 836, 868
Asp700I GAANNNNTTC 2 cut(s) 485, 821
AsuC2I CCSGG 1 cut(s) 150
AsuHPI GGTGA 2 cut(s) 194, 1055
AsuII TTCGAA 1 cut(s) 62
AvaI CYCGRG 1 cut(s) 443
BaeGI GKGCMC 2 cut(s) 111, 733
BanI GGYRCC 2 cut(s) 44, 419
BbsI GAAGAC 1 cut(s) 48
Bbv12I GWGCWC 2 cut(s) 733, 1092
BbvI GCAGC 3 cut(s) 824, 855, 956
BcgI CGANNNNNNTGC 4 cut(s) 572, 606, 623, 657
BciT130I CCWGG 2 cut(s) 267, 1035
BciVI GTATCC 1 cut(s) 358
BcnI CCSGG 1 cut(s) 150
BcoDI GTCTC 2 cut(s) 563, 1020
BcuI ACTAGT 1 cut(s) 368
BfaI CTAG 4 cut(s) 369, 708, 767, 986
BfrI CTTAAG 1 cut(s) 218
BfuI GTATCC 1 cut(s) 358
BisI GCNGC 5 cut(s) 353, 813, 869, 970, 973
BlsI GCNGC 5 cut(s) 354, 814, 870, 971, 974
Bme1390I CCNGG 3 cut(s) 150, 267, 1035
BmeT110I CYCGRG 1 cut(s) 443
BmgBI CACGTC 1 cut(s) 1023
BmiI GGNNCC 4 cut(s) 8, 46, 306, 421
BmrFI CCNGG 3 cut(s) 150, 267, 1035
BmsI GCATC 1 cut(s) 624
BpiI GAAGAC 1 cut(s) 48
BpmI CTGGAG 1 cut(s) 1091
Bpu14I TTCGAA 1 cut(s) 62
BpuMI CCSGG 1 cut(s) 150
Bsa29I ATCGAT 1 cut(s) 894
BsaAI YACGTR 1 cut(s) 1117
BsaBI GATNNNNATC 1 cut(s) 1059
BsaHI GRCGYC 1 cut(s) 636
BsaJI CCNNGG 2 cut(s) 265, 1034
BsaWI WCCGGW 2 cut(s) 395, 422
Bsc4I CCNNNNNNNGG 2 cut(s) 251, 740
Bse118I RCCGGY 3 cut(s) 163, 200, 575
Bse1I ACTGG 1 cut(s) 286
Bse3DI GCAATG 3 cut(s) 158, 301, 588
Bse8I GATNNNNATC 1 cut(s) 1059
BseBI CCWGG 2 cut(s) 267, 1035
BseCI ATCGAT 1 cut(s) 894
BseDI CCNNGG 2 cut(s) 265, 1034
BseGI GGATG 1 cut(s) 543
BseJI GATNNNNATC 1 cut(s) 1059
BseLI CCNNNNNNNGG 2 cut(s) 251, 740
BseMI GCAATG 3 cut(s) 158, 301, 588
BseMII CTCAG 3 cut(s) 324, 403, 449
BseNI ACTGG 1 cut(s) 286
BseRI GAGGAG 1 cut(s) 542
BseSI GKGCMC 2 cut(s) 111, 733
BseXI GCAGC 3 cut(s) 824, 855, 956
BsgI GTGCAG 2 cut(s) 158, 813
BshNI GGYRCC 2 cut(s) 44, 419
BshVI ATCGAT 1 cut(s) 894
BsiHKAI GWGCWC 2 cut(s) 733, 1092
BsiHKCI CYCGRG 1 cut(s) 443
BsiSI CCGG 6 cut(s) 149, 164, 201, 396, 423, 576
BslFI GGGAC 1 cut(s) 623
BslI CCNNNNNNNGG 2 cut(s) 251, 740
BsmAI GTCTC 2 cut(s) 563, 1020
BsmFI GGGAC 1 cut(s) 623
BsoBI CYCGRG 1 cut(s) 443
Bsp119I TTCGAA 1 cut(s) 62
Bsp1286I GDGCHC 3 cut(s) 111, 733, 1092
Bsp143I GATC 5 cut(s) 75, 189, 472, 688, 1039
BspACI CCGC 4 cut(s) 353, 362, 669, 972
BspCNI CTCAG 3 cut(s) 323, 402, 448
BspDI ATCGAT 1 cut(s) 894
BspHI TCATGA 1 cut(s) 325
BspLI GGNNCC 4 cut(s) 8, 46, 306, 421
BspPI GGATC 3 cut(s) 480, 696, 1047
BspT104I TTCGAA 1 cut(s) 62
BspT107I GGYRCC 2 cut(s) 44, 419
BspTI CTTAAG 1 cut(s) 218
BsrDI GCAATG 3 cut(s) 158, 301, 588
BsrFI RCCGGY 3 cut(s) 163, 200, 575
BsrI ACTGG 1 cut(s) 286
BssAI RCCGGY 3 cut(s) 163, 200, 575
BssECI CCNNGG 2 cut(s) 265, 1034
BssMI GATC 5 cut(s) 75, 189, 472, 688, 1039
BssNI GRCGYC 1 cut(s) 636
Bst2UI CCWGG 2 cut(s) 267, 1035
Bst4CI ACNGT 3 cut(s) 557, 789, 1085
Bst6I CTCTTC 1 cut(s) 19
BstACI GRCGYC 1 cut(s) 636
BstAFI CTTAAG 1 cut(s) 218
BstBAI YACGTR 1 cut(s) 1117
BstBI TTCGAA 1 cut(s) 62
BstC8I GCNNGC 1 cut(s) 165
BstDEI CTNAG 4 cut(s) 310, 389, 435, 831
BstF5I GGATG 1 cut(s) 543
BstKTI GATC 5 cut(s) 78, 192, 475, 691, 1042
BstMAI GTCTC 2 cut(s) 563, 1020
BstMBI GATC 5 cut(s) 75, 189, 472, 688, 1039
BstMWI GCNNNNNNNGC 1 cut(s) 704
BstNI CCWGG 2 cut(s) 267, 1035
BstSCI CCNGG 3 cut(s) 148, 265, 1033
BstSLI GKGCMC 2 cut(s) 111, 733
BstSNI TACGTA 1 cut(s) 1117
BstV1I GCAGC 3 cut(s) 824, 855, 956
BstV2I GAAGAC 1 cut(s) 48
BstX2I RGATCY 1 cut(s) 688
BstYI RGATCY 1 cut(s) 688
Bsu15I ATCGAT 1 cut(s) 894
BsuI GTATCC 1 cut(s) 358
BsuTUI ATCGAT 1 cut(s) 894
BtgZI GCGATG 1 cut(s) 597
BtrI CACGTC 1 cut(s) 1023
BtsCI GGATG 1 cut(s) 543
BtsIMutI CAGTG 4 cut(s) 648, 733, 794, 1090
Cac8I GCNNGC 1 cut(s) 165
CaiI CAGNNNCTG 1 cut(s) 691
CciI TCATGA 1 cut(s) 325
Cfr10I RCCGGY 3 cut(s) 163, 200, 575
ClaI ATCGAT 1 cut(s) 894
CseI GACGC 1 cut(s) 818
Csp6I GTAC 2 cut(s) 983, 1114
CviAII CATG 4 cut(s) 193, 326, 847, 1043
CviQI GTAC 2 cut(s) 983, 1114
DdeI CTNAG 4 cut(s) 310, 389, 435, 831
DpnI GATC 5 cut(s) 77, 191, 474, 690, 1041
DpnII GATC 5 cut(s) 75, 189, 472, 688, 1039
DraIII CACNNNGTG 1 cut(s) 129
DrdI GACNNNNNNGTC 1 cut(s) 659
DseDI GACNNNNNNGTC 1 cut(s) 659
Eam1104I CTCTTC 1 cut(s) 19
EarI CTCTTC 1 cut(s) 19
EciI GGCGGA 1 cut(s) 658
Eco105I TACGTA 1 cut(s) 1117
Eco32I GATATC 1 cut(s) 919
Eco57I CTGAAG 3 cut(s) 396, 402, 668
Eco88I CYCGRG 1 cut(s) 443
EcoRI GAATTC 1 cut(s) 777
EcoRII CCWGG 2 cut(s) 265, 1033
EcoRV GATATC 1 cut(s) 919
FaeI CATG 4 cut(s) 196, 329, 850, 1046
FalI AAGNNNNNCTT 2 cut(s) 56, 88
FaqI GGGAC 1 cut(s) 623
FatI CATG 4 cut(s) 192, 325, 846, 1042
FauNDI CATATG 1 cut(s) 358
Fnu4HI GCNGC 5 cut(s) 353, 813, 869, 970, 973
FokI GGATG 1 cut(s) 530
Fsp4HI GCNGC 5 cut(s) 353, 813, 869, 970, 973
FspBI CTAG 4 cut(s) 369, 708, 767, 986
GluI GCNGC 5 cut(s) 353, 813, 869, 970, 973
GsuI CTGGAG 1 cut(s) 1091
HapII CCGG 6 cut(s) 149, 164, 201, 396, 423, 576
HgaI GACGC 1 cut(s) 818
Hin1I GRCGYC 1 cut(s) 636
Hin1II CATG 4 cut(s) 196, 329, 850, 1046
HincII GTYRAC 1 cut(s) 664
HindII GTYRAC 1 cut(s) 664
HinfI GANTC 4 cut(s) 120, 185, 336, 399
HpaII CCGG 6 cut(s) 149, 164, 201, 396, 423, 576
HphI GGTGA 2 cut(s) 194, 1055
Hpy166II GTNNAC 4 cut(s) 664, 731, 901, 1120
Hpy188I TCNGA 5 cut(s) 313, 438, 472, 620, 1020
Hpy188III TCNNGA 5 cut(s) 73, 326, 380, 686, 1108
Hpy8I GTNNAC 4 cut(s) 664, 731, 901, 1120
Hpy99I CGWCG 3 cut(s) 638, 812, 1024
HpyAV CCTTC 3 cut(s) 692, 705, 762
HpyCH4III ACNGT 3 cut(s) 557, 789, 1085
HpyCH4IV ACGT 3 cut(s) 636, 1022, 1116
HpyCH4V TGCA 6 cut(s) 139, 237, 698, 731, 794, 1005
HpyF10VI GCNNNNNNNGC 1 cut(s) 704
HpyF3I CTNAG 4 cut(s) 310, 389, 435, 831
HpySE526I ACGT 3 cut(s) 636, 1022, 1116
Hsp92I GRCGYC 1 cut(s) 636
Hsp92II CATG 4 cut(s) 196, 329, 850, 1046
KroI GCCGGC 1 cut(s) 163
KroNI GCCGGC 1 cut(s) 165
Kzo9I GATC 5 cut(s) 75, 189, 472, 688, 1039
Lsp1109I GCAGC 3 cut(s) 824, 855, 956
LweI GCATC 1 cut(s) 624
MaeI CTAG 4 cut(s) 369, 708, 767, 986
MaeII ACGT 3 cut(s) 636, 1022, 1116
MalI GATC 5 cut(s) 77, 191, 474, 690, 1041
MboI GATC 5 cut(s) 75, 189, 472, 688, 1039
MflI RGATCY 1 cut(s) 688
MhlI GDGCHC 3 cut(s) 111, 733, 1092
MlyI GAGTC 2 cut(s) 345, 408
MmeI TCCRAC 2 cut(s) 302, 1043
MroNI GCCGGC 1 cut(s) 163
MroXI GAANNNNTTC 2 cut(s) 485, 821
MseI TTAA 4 cut(s) 219, 857, 948, 1132
MspCI CTTAAG 1 cut(s) 218
MspI CCGG 6 cut(s) 149, 164, 201, 396, 423, 576
MspR9I CCNGG 3 cut(s) 150, 267, 1035
MvaI CCWGG 2 cut(s) 267, 1035
MwoI GCNNNNNNNGC 1 cut(s) 704
NaeI GCCGGC 1 cut(s) 165
NciI CCSGG 1 cut(s) 150
NdeI CATATG 1 cut(s) 358
NdeII GATC 5 cut(s) 75, 189, 472, 688, 1039
NgoMIV GCCGGC 1 cut(s) 163
NlaIII CATG 4 cut(s) 196, 329, 850, 1046
NlaIV GGNNCC 4 cut(s) 8, 46, 306, 421
NspV TTCGAA 1 cut(s) 62
PagI TCATGA 1 cut(s) 325
PcsI WCGNNNNNNNCGW 1 cut(s) 579
PdiI GCCGGC 1 cut(s) 165
PdmI GAANNNNTTC 2 cut(s) 485, 821
PfeI GAWTC 2 cut(s) 120, 185
PkrI GCNGC 5 cut(s) 354, 814, 870, 971, 974
PleI GAGTC 2 cut(s) 344, 407
PpsI GAGTC 2 cut(s) 344, 407
Ppu21I YACGTR 1 cut(s) 1117
PsiI TTATAA 1 cut(s) 173
Psp6I CCWGG 2 cut(s) 265, 1033
PspGI CCWGG 2 cut(s) 265, 1033
PspN4I GGNNCC 4 cut(s) 8, 46, 306, 421
PstNI CAGNNNCTG 1 cut(s) 691
PsuI RGATCY 1 cut(s) 688
RsaI GTAC 2 cut(s) 984, 1115
RsaNI GTAC 2 cut(s) 983, 1114
SaqAI TTAA 4 cut(s) 219, 857, 948, 1132
SatI GCNGC 5 cut(s) 353, 813, 869, 970, 973
Sau3AI GATC 5 cut(s) 75, 189, 472, 688, 1039
SchI GAGTC 2 cut(s) 345, 408
ScrFI CCNGG 3 cut(s) 150, 267, 1035
SduI GDGCHC 3 cut(s) 111, 733, 1092
SfaNI GCATC 1 cut(s) 624
SfuI TTCGAA 1 cut(s) 62
SmlI CTYRAG 1 cut(s) 218
SmoI CTYRAG 1 cut(s) 218
SnaBI TACGTA 1 cut(s) 1117
SpeI ACTAGT 1 cut(s) 368
SsiI CCGC 4 cut(s) 353, 362, 669, 972
SspMI CTAG 4 cut(s) 369, 708, 767, 986
StyD4I CCNGG 3 cut(s) 148, 265, 1033
TaaI ACNGT 3 cut(s) 557, 789, 1085
TaiI ACGT 3 cut(s) 639, 1025, 1119
TaqI TCGA 3 cut(s) 62, 188, 894
TauI GCSGC 2 cut(s) 355, 975
TfiI GAWTC 2 cut(s) 120, 185
Tru1I TTAA 4 cut(s) 219, 857, 948, 1132
Tru9I TTAA 4 cut(s) 219, 857, 948, 1132
TscAI CASTG 4 cut(s) 655, 733, 794, 1090
TseI GCWGC 3 cut(s) 812, 868, 969
TspDTI ATGAA 5 cut(s) 15, 307, 342, 532, 863
TspRI CASTG 4 cut(s) 655, 733, 794, 1090
Vha464I CTTAAG 1 cut(s) 218
VneI GTGCAC 1 cut(s) 729
XapI RAATTY 4 cut(s) 259, 538, 777, 859
XmnI GAANNNNTTC 2 cut(s) 485, 821
XspI CTAG 4 cut(s) 369, 708, 767, 986
ZraI GACGTC 1 cut(s) 637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.